CN117322419A - Application of jasmone in preparation of parasitic wasp attractant - Google Patents
Application of jasmone in preparation of parasitic wasp attractant Download PDFInfo
- Publication number
- CN117322419A CN117322419A CN202311133962.XA CN202311133962A CN117322419A CN 117322419 A CN117322419 A CN 117322419A CN 202311133962 A CN202311133962 A CN 202311133962A CN 117322419 A CN117322419 A CN 117322419A
- Authority
- CN
- China
- Prior art keywords
- jasmone
- parasitic
- parasitic wasp
- cis
- attractant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- XMLSXPIVAXONDL-PLNGDYQASA-N Jasmone Chemical compound CC\C=C/CC1=C(C)CCC1=O XMLSXPIVAXONDL-PLNGDYQASA-N 0.000 title claims abstract description 63
- XMLSXPIVAXONDL-UHFFFAOYSA-N trans-jasmone Natural products CCC=CCC1=C(C)CCC1=O XMLSXPIVAXONDL-UHFFFAOYSA-N 0.000 title claims abstract description 62
- 230000003071 parasitic effect Effects 0.000 title claims abstract description 51
- 239000005667 attractant Substances 0.000 title claims abstract description 29
- 230000031902 chemoattractant activity Effects 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title description 6
- 241000607479 Yersinia pestis Species 0.000 claims abstract description 24
- 241001481304 Vespoidea Species 0.000 claims abstract description 23
- 241000256259 Noctuidae Species 0.000 claims abstract description 12
- IVLCENBZDYVJPA-ARJAWSKDSA-N cis-Jasmone Natural products C\C=C/CC1=C(C)CCC1=O IVLCENBZDYVJPA-ARJAWSKDSA-N 0.000 claims description 24
- 230000001276 controlling effect Effects 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 7
- 235000010254 Jasminum officinale Nutrition 0.000 claims description 6
- 150000002576 ketones Chemical class 0.000 claims description 5
- 230000001105 regulatory effect Effects 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 4
- 230000024241 parasitism Effects 0.000 claims description 2
- 240000005385 Jasminum sambac Species 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 9
- 241000238631 Hexapoda Species 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 239000002418 insect attractant Substances 0.000 abstract 1
- 241000257303 Hymenoptera Species 0.000 description 14
- YCOZIPAWZNQLMR-UHFFFAOYSA-N pentadecane Chemical compound CCCCCCCCCCCCCCC YCOZIPAWZNQLMR-UHFFFAOYSA-N 0.000 description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 9
- 150000001875 compounds Chemical class 0.000 description 7
- WVDDGKGOMKODPV-UHFFFAOYSA-N Benzyl alcohol Chemical compound OCC1=CC=CC=C1 WVDDGKGOMKODPV-UHFFFAOYSA-N 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 239000003039 volatile agent Substances 0.000 description 6
- 241000207840 Jasminum Species 0.000 description 5
- 230000006399 behavior Effects 0.000 description 5
- 235000013311 vegetables Nutrition 0.000 description 5
- GLZPCOQZEFWAFX-UHFFFAOYSA-N Geraniol Chemical compound CC(C)=CCCC(C)=CCO GLZPCOQZEFWAFX-UHFFFAOYSA-N 0.000 description 4
- 239000005662 Paraffin oil Substances 0.000 description 4
- NDJKXXJCMXVBJW-UHFFFAOYSA-N heptadecane Chemical compound CCCCCCCCCCCCCCCCC NDJKXXJCMXVBJW-UHFFFAOYSA-N 0.000 description 4
- JARKCYVAAOWBJS-UHFFFAOYSA-N hexanal Chemical compound CCCCCC=O JARKCYVAAOWBJS-UHFFFAOYSA-N 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 239000003995 emulsifying agent Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000000422 nocturnal effect Effects 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- -1 synergist Substances 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- GLZPCOQZEFWAFX-YFHOEESVSA-N Geraniol Natural products CC(C)=CCC\C(C)=C/CO GLZPCOQZEFWAFX-YFHOEESVSA-N 0.000 description 2
- 239000005792 Geraniol Substances 0.000 description 2
- 239000004166 Lanolin Substances 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 244000269722 Thea sinensis Species 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 235000019445 benzyl alcohol Nutrition 0.000 description 2
- JHIVVAPYMSGYDF-UHFFFAOYSA-N cyclohexanone Chemical compound O=C1CCCCC1 JHIVVAPYMSGYDF-UHFFFAOYSA-N 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 229940113087 geraniol Drugs 0.000 description 2
- 229940039717 lanolin Drugs 0.000 description 2
- 235000019388 lanolin Nutrition 0.000 description 2
- 150000007823 ocimene derivatives Chemical class 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 239000003208 petroleum Substances 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 229940051841 polyoxyethylene ether Drugs 0.000 description 2
- 229920000056 polyoxyethylene ether Polymers 0.000 description 2
- 229920000136 polysorbate Polymers 0.000 description 2
- 238000007619 statistical method Methods 0.000 description 2
- 239000000375 suspending agent Substances 0.000 description 2
- XJPBRODHZKDRCB-UHFFFAOYSA-N trans-alpha-ocimene Natural products CC(=C)CCC=C(C)C=C XJPBRODHZKDRCB-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- OGLDWXZKYODSOB-UHFFFAOYSA-N α-phellandrene Chemical compound CC(C)C1CC=C(C)C=C1 OGLDWXZKYODSOB-UHFFFAOYSA-N 0.000 description 2
- 239000001169 1-methyl-4-propan-2-ylcyclohexa-1,4-diene Substances 0.000 description 1
- KJYPHTKJXTUHCJ-UHFFFAOYSA-N 3,7,11-trimethyldodeca-1,3,6,10-tetraene Chemical compound CC(C=C)=CCC=C(CCC=C(C)C)C.CC(C)=CCCC(C)=CCC=C(C)C=C KJYPHTKJXTUHCJ-UHFFFAOYSA-N 0.000 description 1
- GWYFCOCPABKNJV-UHFFFAOYSA-M 3-Methylbutanoic acid Natural products CC(C)CC([O-])=O GWYFCOCPABKNJV-UHFFFAOYSA-M 0.000 description 1
- 241001124076 Aphididae Species 0.000 description 1
- 241001305034 Aphidius gifuensis Species 0.000 description 1
- QJCLSABFTQBZCG-UHFFFAOYSA-N C=CC(CC)O.C=CC(CC)O Chemical compound C=CC(CC)O.C=CC(CC)O QJCLSABFTQBZCG-UHFFFAOYSA-N 0.000 description 1
- OLFQLEJXIHEDEJ-UHFFFAOYSA-N CCCCC(CC)CO.CC(C)CCCCCO Chemical compound CCCCC(CC)CO.CC(C)CCCCCO OLFQLEJXIHEDEJ-UHFFFAOYSA-N 0.000 description 1
- 241000426497 Chilo suppressalis Species 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 229920001732 Lignosulfonate Polymers 0.000 description 1
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000222351 Pleurotus cornucopiae Species 0.000 description 1
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical class CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 1
- 241000256618 Trichogramma Species 0.000 description 1
- 241000256856 Vespidae Species 0.000 description 1
- OGLDWXZKYODSOB-SNVBAGLBSA-N alpha-phellandrene Natural products CC(C)[C@H]1CC=C(C)C=C1 OGLDWXZKYODSOB-SNVBAGLBSA-N 0.000 description 1
- 239000001387 apium graveolens Substances 0.000 description 1
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- GWYFCOCPABKNJV-UHFFFAOYSA-N beta-methyl-butyric acid Natural products CC(C)CC(O)=O GWYFCOCPABKNJV-UHFFFAOYSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- RGACQXBDYBCJCY-ALCCZGGFSA-N cis-3-Hexenyl hexanoate Chemical compound CCCCCC(=O)OCC\C=C/CC RGACQXBDYBCJCY-ALCCZGGFSA-N 0.000 description 1
- RGACQXBDYBCJCY-UHFFFAOYSA-N cis-3-Hexenyl-n-caproat Natural products CCCCCC(=O)OCCC=CCC RGACQXBDYBCJCY-UHFFFAOYSA-N 0.000 description 1
- AIQLNKITFBJPFO-UHFFFAOYSA-N cis-3-Hexenylisovalerat Natural products CCC=CCCOC(=O)CC(C)C AIQLNKITFBJPFO-UHFFFAOYSA-N 0.000 description 1
- AIQLNKITFBJPFO-WAYWQWQTSA-N cis-3-hexenyl isovalerate Chemical compound CC\C=C/CCOC(=O)CC(C)C AIQLNKITFBJPFO-WAYWQWQTSA-N 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- GVGUFUZHNYFZLC-UHFFFAOYSA-N dodecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 GVGUFUZHNYFZLC-UHFFFAOYSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000004495 emulsifiable concentrate Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000002334 glycols Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- FUZZWVXGSFPDMH-UHFFFAOYSA-N n-hexanoic acid Natural products CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- 230000034267 olfactory behavior Effects 0.000 description 1
- 150000007875 phellandrene derivatives Chemical class 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 229950008882 polysorbate Drugs 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- HSJXWMZKBLUOLQ-UHFFFAOYSA-M potassium;2-dodecylbenzenesulfonate Chemical compound [K+].CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O HSJXWMZKBLUOLQ-UHFFFAOYSA-M 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 230000009329 sexual behaviour Effects 0.000 description 1
- 229940080264 sodium dodecylbenzenesulfonate Drugs 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000013268 sustained release Methods 0.000 description 1
- 239000012730 sustained-release form Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000004506 ultrasonic cleaning Methods 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P19/00—Pest attractants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Plant Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Insects & Arthropods (AREA)
- Catching Or Destruction (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Compared with the existing insect attractants in the market at present, the jasmone attractant provided by the invention is an attractive biological parasitic bee which is trapped, so that the worry that a planter is concerned about trapping more insects in the field can be eliminated, and the field control effect is better as more beneficial insects are trapped. Besides the light foot side ditch cocoons, the parasitic wasp attractant provided by the invention not only has an trapping effect on the parasitic wasps of noctuidae pests, but also can trap other parasitic wasps, so that the number of the parasitic wasps in the field is increased, the field parasitic rate of the parasitic wasps is improved, and the effect of controlling the field pests by the parasitic wasps is achieved.
Description
Technical Field
The invention relates to the technical field of biological pesticides, such as natural enemy attractants, in particular to application of jasmone in preparation of parasitic wasp attractants.
Background
Parasitic wasps are important parasitic natural enemy insects and play an important role in green prevention and control of crop pests. The parasitic wasp and its offspring usually regulate the physiological state of the host through parasitic behavior, ensure the parasitic wasp offspring develop normally in the host body surface or body, make its population reproduce, and the host pest is controlled effectively. At present, various parasitic bees are successfully commercialized and applied to biological control of rice and vegetable pests, such as trichogramma for controlling rice stem borers, aphidius gifuensis for controlling vegetable aphids and the like. Micropplitispa llipes on the side ditch of the light foot is taken as a dominant parasitic wasp which is the low-age larva in vegetable noctuid, and 2-3-age noctuid larva with clustering habit is parasitic, so that the parasitic wasp is an important regulating factor for the natural population quantity of noctuid insect pests in fields.
However, for parasitic wasps, whether the host can be found and identified accurately and parasitic successfully, directly determines the survival and reproduction of its offspring population. In the past, it has been found that parasitic wasps search for hosts by identifying, primarily through smell, chemical information substances associated with the host, and that such information substances have a variety of effective sources, such as pest-induced plant volatiles, the host itself, and faeces thereof. Therefore, the efficiency of parasitic wasps searching hosts is effectively regulated and controlled by exploring volatile odor substances related to the trending behaviors of the light foot side hornet so as to achieve the aim of controlling target pests, and a novel green means and technology are provided for green prevention and control of the pests. Compared with other pest control technologies, the novel natural enemy olfactory behavior regulation technology has the advantages of environment friendliness, no public hazard, safety, high efficiency, high selectivity and the like, and has a wide application prospect in pest comprehensive control.
Disclosure of Invention
The invention aims to provide a preparation method and application of a jasmone parasitic wasp attractant, which can attract parasitic wasps, improve the searching and positioning capability of the parasitic wasps on target pests, further improve the parasitic efficiency of the parasitic wasps, realize effective biological prevention and control of the pests, reduce the application of chemical pesticides and ensure the safety of vegetable products.
The invention aims at realizing the following specific technical scheme:
the application of jasmone in preparing parasitic wasp attractant.
Optionally, the content of the jasmone is 0.5% -10%.
Optionally, the content of the jasmone is 1.0%.
Optionally, the content of the jasmone is 5.0%.
Optionally, the parasitic wasp is a light foot side cocoon bee.
Optionally, the jasmone is cis-jasmone.
A jasmine ketone parasitic wasp attractant comprises the following components in percentage by mass: 0.5 to 99 percent of cis-jasmone; 1 to 99.5 percent of conventional auxiliary materials.
Optionally, the content of the cis-jasmone is 1.0% -10%.
Optionally, the content of cis-jasmone is 5.0%.
The invention discloses a method for preventing and controlling noctuidae pests, which utilizes any jasmine ketone parasitic wasp attractant to induce and enrich parasitic wasps, thereby carrying out the parasitism of 2-3-year-old noctuidae larvae and regulating and controlling the number of the noctuidae pests.
Compared with the prior art, the invention has the following beneficial effects:
compared with the existing pest attractants in the current market, the jasmine ketone attractant provided by the invention is an attractive parasitic wasp, can eliminate the worry that a planter worries about attracting more pests to the field of the planter, and has better field control effect as more beneficial insects are. Besides the light foot side ditch cocoons, the parasitic wasp attractant provided by the invention not only has an trapping effect on the parasitic wasps of noctuidae pests, but also can trap other parasitic wasps, so that the number of the parasitic wasps in the field is increased, the field parasitic rate of the parasitic wasps is improved, and the effect of controlling the field pests by the parasitic wasps is achieved.
The cis-jasmone adopted by the invention is used as an trapping volatile matter, is a natural plant product, has the characteristics of low toxicity, easy degradation and the like, and is an attractant friendly and safe to human and livestock and ecology.
In the present invention, cis-jasmone can be obtained by various methods such as chemical synthesis, direct extraction from plants, etc., and can be industrially produced on a large scale.
Drawings
The accompanying drawings are included to provide a further understanding of the disclosure, and are incorporated in and constitute a part of this specification, illustrate the disclosure and together with the description serve to explain, but do not limit the disclosure. In the drawings:
fig. 1 is a structural diagram of a "Y" type olfactometer.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the present invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications could be made by those skilled in the art without departing from the inventive concept. These are all within the scope of the present invention.
The application of jasmone in preparing parasitic wasp attractant. For example, the content of jasmone may be 0.5% -10%. For example, the content of jasmone may be 1.0%. For example, the content of jasmone may be 5.0%.
Specifically, the parasitic wasp is a light foot side ditch cocoon bee; the jasmone is cis-jasmone.
Cis-jasmone ((Z) -3-methyl-2- (2-pentenyl) -2-cyclopenten-1-one, molecular formula is C 11 H 16 O, molecular weight: 164.25; CAS number: 488-10-8; the structural formula of the light yellow liquid is shown as follows, is an essence with elegant jasmine fragrance and celery seed fragrance, and is widely used in food and daily chemical high-grade essence. At present, no report on the application of cis-jasmone in attracting side ditch cocoon bees to control vegetable noctuid pests is known.
The invention provides a jasmone parasitic wasp attractant, which comprises the following components in percentage by mass:
0.5 to 99 percent of cis-jasmone;
1 to 99.5 percent of conventional auxiliary materials.
In the jasmone parasitic wasp attractant, the jasmone is used as an active substance, the jasmone is cis-jasmone, and the parasitic wasp is the foot-less side sulcus vesiculosus.
The conventional auxiliary materials can be one or more of solubilizer, synergist, emulsifier and the like; the solubilizer is one or more of sodium dodecyl benzene sulfonate, potassium dodecyl benzene sulfonate, polysorbate, polyethylene glycol, sorbitol polyoxyethylene ether, lignosulfonate, tea seed cake, fructus Gleditsiae Abnormalis, etc.; the emulsifier is span, tween, glycol esters, propylene glycol esters and/or polyethylene glycol nonionic surfactant; the synergist is tea saponin, polyoxyethylene ether, etc.
The preparation method of the jasmone parasitic wasp attractant comprises the following steps:
(1) Preparing a jasmone parasitic wasp attractant by taking the jasmone as an active substance; the jasmone is prepared by diluting with paraffin oil, lanolin or ethanol;
(2) Weighing cis-jasmone according to the proportion, dissolving the cis-jasmone by using a solvent, adding various auxiliary agents, and preparing various forms of attractants according to various processes, for example: powder, emulsifiable concentrate, suspending agent, dry suspending agent, concentrated emulsion, sustained release agent, nanometer preparation, aqueous emulsion, spray and the like. The solvent can be one or more of paraffin oil, lanolin, water, ethanol, methanol, acetone, cyclohexanone, petroleum ether, petroleum aromatic hydrocarbon, pure benzene, ethyl acetate, toluene, xylene, etc.
The invention discloses a method for preventing and controlling nocturnal moth pests, which utilizes any jasmine ketone parasitic wasp attractant to attract and enrich parasitic wasps, thereby carrying out the parasitic wasp of 2-3-year-old nocturnal moth larvae and regulating and controlling the number of nocturnal moth pests.
In the invention, a Y-type olfactometer method is adopted to test the trend reaction of the light foot side cocoon bees in a laboratory.
The specific method comprises the following steps: as shown in FIG. 1, 250mL jar with frosted mouth was used as the flavor source jar for placing different treatment groups. The two arms and the release arm of the olfactory instrument are 15cm long, the inner diameter is 1.8cm, and the included angle between the two arms is 60 degrees. In order to avoid the influence of uneven surrounding light intensity on the sexual behavior of parasitic bees, an olfactometer is placed in a square box made of metal wires, the periphery of the square box is covered by waxed paper (80 cm multiplied by 50 cm), and a 40W incandescent lamp is placed right above the box to make the light intensity of two test arms consistent. The two arms of the Y-shaped olfactory instrument are respectively connected with the odor source bottle through a silicone tube. Before the air flow enters the flavor source bottle, the air flow is filtered by the activated carbon and the distilled water bottle to purify the air and increase the air humidity. The air-time under test was 200mL/min.
The test was arranged at 8:30 am to 5:00 pm daily, based on the biological properties of the light foot side sulcus. Introducing female bees of the light foot side ditch bees from the tail end of a base tube of the Y-shaped olfactometer head by head, observing and recording the behavioral response of the parasitic bees within 5 minutes. The decision criteria are as follows: when the parasitic wasp climbs to the exit or exceeds 1/3 of a certain arm and stays for more than 5s, the wasp is recorded as selecting the smell source of the arm; if the bee is introduced for 5min, the selection is still not made, and the behavior observation of the bee is finished. Every 5 heads are measured, the orientation of the olfactometer is exchanged once so as to eliminate the influence of the geometrical position on parasitic wasp behaviors. For each 10 heads, the olfactometer was cleaned with 95% ethanol and blow-dried with a blower and cooled. 60 bees were measured per treatment. When the treatment is replaced, the ultrasonic cleaning device is used, and 95% ethanol is used for cleaning and drying for standby.
Parasitic wasp selectivity = number of parasitic wasp selective volatile compounds/number of total parasitic wasp tested 100
Example 1: attraction of different compounds to light foot side ditch cocoon bees
Weighing 1g of volatile compound, adding 1ml of Tween 80 emulsifier, finally dripping paraffin oil, dissolving and diluting to 100ml, uniformly mixing to prepare the attractant, adjusting the content of the volatile compound to 0.1g/ml (10%), and then selecting a Y-type olfactometer selection method for selectivity determination, wherein the selection effect of parasitic wasps on each volatile is shown in table 1. The source of volatile compounds in this test was the standard purchased by the reagent company. Volatile compounds include: alpha-Farnesene (alpha-Farnesene), phellandrene (alpha-phellandrene), ocimene (Ocimene), geraniol (Geraniol), cis-Jasmone (cis-Jasmone), isooctanol (2-Ethyl hexanol), caproic acid leaf alcohol ester (cis-3-Hexenyl hexanoate), isovaleric acid leaf alcohol ester (cis-3-Hexenyl isovalerate), heptadecane (Heptadecane), 1-Penten-3-ol (1-Penten-3-ol), hexanal (Caproaldehyde), benzyl alcohol (Benzyl alcohol), pentadecane (Pentadecane).
TABLE 1 Selectivity of light lateral sulcus vesiculosus to different volatile compounds
As can be seen from Table 1, the light foot side cocoon bees exhibit different degrees of selectivity for 13 different types of volatile compounds measured in the present invention. Wherein the selectivity of the parasitic wasp to cis-jasmone and pentadecane is relatively high, namely 64.81% and 64.29%, respectively. At the same time, the statistical analysis results showed that only cis-jasmone and pentadecane showed a significant difference in selectivity to the parasitic wasp (P value < 0.05) compared to the blank control.
Example 2: attraction of jasmone with different concentrations to light foot side ditch cocoon bees
This example is different from example 1 in that the volatile compound is cis-jasmone, cis-jasmone is measured, and the volatile compound is added dropwise into paraffin oil to be dissolved, so as to prepare attractants with mass concentrations of 0.005 (0.5%), 0.01 (1.0%), 0.05 (5.0%), 0.1 (10%), 0.5 (50%), and 0.98g/mL (98%), respectively; the effect of parasitic wasps on each volatiles selection is shown in table 2:
TABLE 2 influence of different concentrations of jasmone on the selectivity of Pleurotus cornucopiae
The results in table 2 illustrate: the selectivity of the light foot side cocoon bees to the jasmone is higher in the mass concentration range of 1.0-10%, and the selectivity is more than 64%. However, when the concentration of jasmone exceeds 10%, the trapping ability of the milpa thereto is not remarkably enhanced but is reduced to various degrees with the increase of the concentration of jasmone. In practical application, the two aspects of trapping effect and cost are comprehensively considered, and the proper dosage is selected.
The experimental results of the embodiment 1 and the embodiment 2 show that the selectivity of parasitic wasps to cis-jasmone and pentadecane is higher in 13 different types of volatile compounds tested by the invention, the statistical analysis results also show obvious differences, and the attraction rate reaches more than 64% when the mass concentration is 10%; in addition, through the selective experiments of different concentrations of jasmone, the selectivity of parasitic bees can reach more than 64% at the mass concentration of 1% -10%, and the parasitic bees can be used as a parasitic bee attractant.
The foregoing describes specific embodiments of the present invention. It is to be understood that the present invention is not limited to the specific embodiments described above, and that other examples may be obtained by varying the component content in the examples described above. Various changes or modifications may be made by one skilled in the art within the scope of the claims without affecting the spirit of the invention.
The preferred embodiments of the present disclosure have been described in detail above with reference to the accompanying drawings, but the present disclosure is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solutions of the present disclosure within the scope of the technical concept of the present disclosure, and all the simple modifications belong to the protection scope of the present disclosure.
In addition, the specific features described in the foregoing embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, the present disclosure does not further describe various possible combinations.
Claims (10)
1. The application of jasmone in preparing parasitic wasp attractant.
2. The use according to claim 1, wherein the jasmone content is 0.5% to 10%.
3. The use according to claim 1, wherein the jasmone content is 1.0%.
4. The use according to claim 1, wherein the jasmone content is 5.0%.
5. The use according to claim 1, 2 or 3, wherein the parasitic wasp is a light foot side cocoon bee.
6. The use according to claim 1, 2 or 3, wherein the jasmone is cis-jasmone.
7. The jasmine ketone parasitic wasp attractant is characterized by comprising the following components in percentage by mass:
0.5 to 99 percent of cis-jasmone;
1 to 99.5 percent of conventional auxiliary materials.
8. The jasmone parasitic wasp attractant of claim 7, wherein the cis-jasmone is present in an amount of 1.0% to 10%.
9. The jasmone parasitic wasp attractant of claim 7, wherein said cis-jasmone is present in an amount of 5.0%.
10. A method for controlling noctuidae pests, characterized in that the number of noctuidae pests is regulated and controlled by using the jasmone parasitic wasp attractant as defined in any one of claims 7-9 to induce and enrich parasitic wasps, thereby carrying out the parasitism of 2-3-year-old noctuidae larvae.
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