CN116891862B - Zoysia japonica salt tolerance gene ZmLA1, protein and application thereof - Google Patents

Zoysia japonica salt tolerance gene ZmLA1, protein and application thereof Download PDF

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CN116891862B
CN116891862B CN202311060158.3A CN202311060158A CN116891862B CN 116891862 B CN116891862 B CN 116891862B CN 202311060158 A CN202311060158 A CN 202311060158A CN 116891862 B CN116891862 B CN 116891862B
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李晓慧
徐京亚
王浩然
张苓
郭海林
李建建
姚祥
郝东利
孔维一
陈静波
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Abstract

The invention provides zoysia japonica salt-tolerant gene ZmLA1 and protein and application thereof, belonging to the technical field of genetic engineering. The invention clones a new zoysia japonica salt-tolerance related gene ZmLA1 for the first time, and the nucleotide sequence is shown as SEQ ID NO. 1. The zoysia japonica salt tolerance related gene ZmLA1 is introduced into plants, so that the salt tolerance of the plants can be improved, and the zoysia japonica salt tolerance related gene ZmLA1 can be applied to genetic improvement of the plants. The zoysia japonica salt-tolerance related gene ZmLA1 is constructed into a plant expression vector, and genetic transformation is carried out by an agrobacterium-mediated method, so that a new germplasm with salt-tolerance characteristic can be obtained, and the zoysia japonica salt-tolerance related gene ZmLA1 has important significance for cultivation of new varieties of good crops and wide application in production.

Description

Zoysia japonica salt tolerance gene ZmLA1, protein and application thereof
Technical Field
The invention belongs to the technical field of genetic engineering, and particularly relates to a zoysia japonica salt-tolerant gene ZmLA1, protein and application thereof.
Background
Zoysia matrella (Zoysia matrella), a perennial warm season turf grass, belongs to the genus Zoysia of the subfamily poaceae (Gramineae) and the subfamily penoxsulae. Zoysia has a specific double-cell salina gland structure, is one of the most salt-tolerant C4 ecological grasses, and has the characteristics of very strong stress resistance, drought resistance, salt and alkali resistance, high wear resistance, high cold resistance and the like.
According to incomplete statistics of the national science and education organizations and the world grain and agriculture organizations, the total land area of the world is about 133.9 hundred million hectares, wherein the saline-alkali land area exceeds 8 hundred million hectares, and the saline-alkali land area accounts for 5.9% of the total land area of the world. Soil salinization is a global problem, about 20% of available cultivated land worldwide has been affected by salinity, and about 10% increase per year, it being estimated that about half of the cultivated land area will be affected by soil salinization in year 2050. Soil salinization has become a key stress factor affecting plant growth and development and yield.
In the aspect of saline-alkali soil development and utilization, the improvement of soil adaptation plants is changed into the selection of plants adaptation soil. The cultivation and utilization of salt tolerant plants is the most advantageous way to solve the plant growth under salt stress. Along with the continuous perfection of molecular biology technology, the improvement of plant stress resistance by combining a genetic engineering means with a molecular design concept has become a hot spot direction of current research. Thus, it is important to excavate and use the salt tolerance gene.
Disclosure of Invention
In view of the above, the present invention aims to provide a zoysia japonica salt tolerance gene ZmLA1, protein and application thereof.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a zoysia japonica salt-tolerant gene ZmLA1, wherein the nucleotide sequence of the salt-tolerant gene ZmLA1 is shown as SEQ ID NO. 1.
The invention also provides a zoysia japonica salt-tolerant protein, and the amino acid sequence of the salt-tolerant protein is shown as SEQ ID NO. 2.
The invention also provides a recombinant expression vector which contains the salt-tolerant gene ZmLA1.
Preferably, the backbone of the vector comprises pCAMBIA1305.
The invention also provides an expression cassette, a transgenic cell line, recombinant bacteria or recombinant viruses containing the salt-tolerant gene ZmLA1.
The invention also provides application of the salt-tolerant gene ZmLA1, the salt-tolerant protein, the recombinant expression vector or the expression cassette, a transgenic cell line, recombinant bacteria or recombinant viruses in improving the salt tolerance of plants.
The invention also provides application of the salt-tolerant gene ZmLA1, the salt-tolerant protein, the recombinant expression vector or the expression cassette, a transgenic cell line, recombinant bacteria or recombinant viruses in salt-tolerant plant breeding.
The invention also provides a method for cultivating salt-tolerant transgenic plants, which comprises the following steps: the salt-tolerant gene ZmLA1 is introduced into plants to obtain transgenic plants.
Preferably, the method of introducing comprises effecting the recombinant expression vector described above or the expression cassette described above, a transgenic cell line, a recombinant bacterium or a recombinant virus.
Preferably, the plant comprises tobacco, centella asiatica, arabidopsis thaliana, rice, wheat, maize, cucumber, tomato, poplar, turf grass or alfalfa.
The invention has the beneficial effects that:
the invention clones a new zoysia japonica salt-tolerance related gene ZmLA1 for the first time, and introduces the zoysia japonica salt-tolerance related gene ZmLA1 into plants, thereby improving the salt tolerance of the plants and being applicable to genetic improvement of the plants. The zoysia japonica salt-tolerance related gene ZmLA1 is constructed into a plant expression vector, and genetic transformation is carried out by an agrobacterium-mediated method, so that a new germplasm with salt-tolerance characteristic can be obtained, and the zoysia japonica salt-tolerance related gene ZmLA1 has important significance for cultivation of new varieties of good crops and wide application in production.
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FIG. 1 shows the result of ZmLA1 agarose gel electrophoresis analysis, wherein M is DL2000 DNA Marker, and 1 is ZmLA1;
FIG. 2 shows the results of double digestion of pCAMBIA1305 vector plasmids XbaI and BamHI, wherein M is DL5000 DNA Marker and 1 is a double enzyme tangential fragment of pCAMBIA1305 plasmid;
FIG. 3 shows the results of PCR identification of Wild Type (WT) and pCAMBIA1305-ZmLA1 transgenic Arabidopsis thaliana (ZmLA 1), where M is DL2000 DNA Marker, "+" is positive control, "-" is wild type negative control, OE1-OE4 is transgenic Arabidopsis thaliana positive plant;
FIG. 4 shows the salt tolerance evaluation results of wild-type and transgenic Arabidopsis thaliana, wherein A is the growth phenotype of the wild-type Col and the transgenic Arabidopsis thaliana root system before and after NaCl treatment, wherein OE1 and OE2 are independent transgenic Arabidopsis thaliana strains, B is the total length statistics of the wild-type and transgenic Arabidopsis thaliana root systems before and after NaCl treatment, and C is the surface area statistics of the wild-type and transgenic Arabidopsis thaliana root systems before and after NaCl treatment.
Detailed Description
The invention provides a zoysia japonica salt-tolerant gene ZmLA1, wherein the nucleotide sequence of the salt-tolerant gene ZmLA1 is shown as SEQ ID NO. 1. The invention also provides a zoysia japonica salt-tolerant protein, and the amino acid sequence of the salt-tolerant protein is shown as SEQ ID NO. 2.
The invention also provides a recombinant expression vector which contains the salt-tolerant gene ZmLA1.
In the present invention, the backbone of the vector preferably includes pCAMBIA1305, and the present invention is not particularly limited to the specific source of pCAMBIA1305, and any commercially available products are available in the art. In the present invention, the recombinant expression vector is preferably a recombinant plasmid obtained by inserting the salt-tolerant gene ZmLA1 into the recombinant site of the restriction enzyme XbaI and BamHI double restriction enzyme vector pCAMBIA1305, and in a specific example, pCAMBIA1305 containing ZmLA1 is named pCAMBIA1305-ZmLA1.
In the present invention, a recombinant expression vector containing the gene can be constructed using existing plant expression vectors. The plant expression vector preferably comprises a binary agrobacterium vector and a vector which can be used for plant microprojectile bombardment. The plant expression vector may also comprise the 3' -untranslated region of a foreign gene, i.e., comprising a polyadenylation signal and any other DNA segments involved in mRNA processing or gene expression. The polyadenylation signal directs the addition of polyadenylation to the 3 'end of the mRNA precursor, and may be similarly functional in the untranslated regions transcribed from the 3' end of, for example, agrobacterium tumefaciens induction (Ti) plasmid genes (e.g., nopaline synthase Nos genes) and plant genes (e.g., soybean storage protein genes). When the gene is used for constructing a recombinant plant expression vector, any one of an enhanced promoter or a constitutive promoter such as a cauliflower mosaic virus (CAMV) 35S promoter can be added before the transcription initiation nucleotide thereof, and the enhanced promoter or the constitutive promoter can be used alone or in combination with other plant promoters; in addition, when constructing a plant expression vector using the gene of the present invention, enhancers, including translational or transcriptional enhancers, may be used, and these enhancers may be ATG initiation codons or adjacent region initiation codons, but must be identical to the reading frame of the coding sequence to ensure proper translation of the entire sequence. The sources of the translational control signals and initiation codons are broad, and can be either natural or synthetic. The translation initiation region may be derived from a transcription initiation region or a structural gene.
The invention also provides an expression cassette, a transgenic cell line, recombinant bacteria or recombinant viruses containing the salt-tolerant gene ZmLA1. The specific type of the expression cassette, transgenic cell line, recombinant bacterium or recombinant virus is not particularly limited in the present invention.
The invention also provides application of the salt-tolerant gene ZmLA1, the salt-tolerant protein, the recombinant expression vector or the expression cassette, a transgenic cell line, recombinant bacteria or recombinant virus in improving the salt tolerance of plants or in breeding salt-tolerant plants.
The invention also provides a method for cultivating salt-tolerant transgenic plants, which comprises the following steps: the salt-tolerant gene ZmLA1 is introduced into plants to obtain transgenic plants.
In the present invention, the method of introducing preferably comprises the implementation by the above recombinant expression vector or the above expression cassette, transgenic cell line, recombinant bacterium or recombinant virus. After introduction into plants, the plant expression vectors used are preferably processed, for example by adding genes which code for enzymes or luminescent compounds which produce a color change (GUS genes, luciferase genes), antibiotic markers which are resistant (gentamicin markers, kanamycin markers) or marker genes which are resistant to chemicals (e.g.herbicide genes) which are expressed in plants, in order to facilitate the identification and selection of transgenic plants. From the safety of transgenic plants, transformed plants can be screened directly in stress without adding any selectable marker gene. In the present invention, the plant preferably includes monocotyledonous plants and dicotyledonous plants, more preferably includes tobacco, baccarat, arabidopsis, rice, wheat, maize, cucumber, tomato, poplar, turf grass or alfalfa.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
In the following examples, conventional methods are used unless otherwise specified.
Materials, reagents and the like used in the examples described below are commercially available unless otherwise specified.
Example 1
Cloning of zoysia japonica ZmLA1
Selecting Zoysia matrella stolons as a material, placing the Zoysia matrella stolons in a 1/2Hoagland nutrient solution for water culture for 30 days, transferring the Zoysia matrella stolons into the 1/2Hoagland nutrient solution containing 250mM NaCl for treatment for 7d, taking 0.1g of tender leaves, extracting total RNA of the leaves according to a Trizol RNA extraction kit (TaKaRa) instruction method, taking 1 mug of total RNA according to an M-MLV reverse transcription kit (TaKaRa) for reverse transcription into cDNA, digesting the cDNA product by RNase, designing primers, and the sequence is as follows:
Primer1：5'ATGATGATGAAGAAGGCTTCGT 3'(SEQ ID NO.3)；
Primer2：5'TCAGAGTCCAAGGAGCTGGC 3'(SEQ ID NO.4)。
PCR reaction was performed using Primer1 and Primer2 as primers and Hamamelis virgata leaf cDNA as a template, and a 20. Mu.L reaction system: 2 XLA RCRMix 10. Mu.L, primer1, primer2 primers 1.0. Mu.L each (10. Mu. Mol.L) -1 ) cDNA template 1. Mu.L, ddH 2 O7 μl; the reaction procedure: 94 ℃ for 3min;94℃30sec,55℃1min,72℃2min,35 cycles; and at 72℃for 5min. The result of agarose gel electrophoresis analysis of the PCR product is shown in FIG. 1, and the PCR product was recovered and purified using a gel recovery kit (AXYGEN). After recovery and purification, the cells were connected to pMD18-T (Japanese Takara Co., ltd.) and E.coli DH 5. Alpha. Competent cells (Beijing Tiangen Co., ltd. CB 101) were transformed, and positive clones were selected and sequenced.
The sequence determination result shows that the nucleotide sequence of the fragment obtained by the PCR reaction is shown as SEQ ID NO.1, and the protein consisting of 357 amino acid residues is encoded (the amino acid sequence of the protein is shown as SEQ ID NO. 2). The protein shown in SEQ ID No.2 is named ZmLA1, and the coding gene for the protein is named ZmLA1.
Example 2
Construction of recombinant expression vectors
Designing a primer, and carrying out PCR amplification to obtain a target sequence with a recombinant adaptor, wherein the sequence of the PCR primer is as follows:
Primer3：5'CGGAGCTAGCTCTAGAATGATGATGAAGAAGGCTTCGT 3'(SEQ ID NO.5)；
Primer4：5'TGCTCACCATGGATCCTCAGAGTCCAAGGAGCTGGC 3'(SEQ ID NO.6)。
the positive sequencing plasmid extracted in example 1 was used as template with high fidelity enzyme (PrimeSTAR TM HSDNA Polymerase, taKaRa), 25 μl reaction system: 10 XHS RCR Buffer 2.5. Mu.L, primer3, primer4 primers 1.0. Mu.L each (10. Mu. Mol.L) -1 )，dNTP mix 2.0μL(2.5mmol·L -1 )，PrimeSTAR TM HS DNA Polymerase 0.2.2. Mu.L, cDNA template 1. Mu.L, ddH 2 O17.3 μl; the reaction procedure: pre-denaturation at 95℃for 3min, then melting at 94℃for 30sec, annealing at 60℃for 30sec, extension at 72℃for 2min, reaction for 35 cycles, extension at 72℃for 10min.
The vector pCAMBIA1305 was digested with XbaI and BamHI, and the results are shown in FIG. 2. The vector cleavage products were recovered using a gel recovery kit (AXYGEN, USA).
The PCR product was cloned into the vector pCAMBIA1305 using INFUSION recombination kit (Japanese Takara). INFUSION recombinant reaction System (10. Mu.L): PCR product 2.0. Mu.L, pCAMBIA 13055.0. Mu.L, 5 Xinfusionbuffer 2.0. Mu.L, infusion enzyme mix. Mu.L. After brief centrifugation, the mixture was subjected to a water bath at 37℃for 15 minutes and then a water bath at 50℃for 15 minutes, and 2.5. Mu.L of the reaction system was used to transform E.coli DH 5. Alpha. Competent cells (Tiangen, beijing; CB 101) by heat shock. All the transformed cells were uniformly plated on LB solid medium containing 50mg/L kanamycin. After 16h incubation at 37℃the clone positive clones were picked and sequenced. As a result of sequencing, it was revealed that a recombinant expression vector containing the gene shown in SEQ ID NO.1 was obtained, and the pCAMBIA1305 containing ZmLA1 was named pCAMBIA1305-ZmLA1, and the ZmLA1 gene fragment was inserted between the XbaI and BamHI cleavage sites of the vector using INFUSION recombination kit (Japanese Takara).
Example 3
Arabidopsis genetic transformation and PCR identification
(1) Agrobacterium strain EHA105 freeze thawing transformation: mu.L of EHA105 competent cells (purchased from the company Jongjun, U.S.A.) were added to 5. Mu.L of the vector plasmid pCAMBIA1305-ZmLA1, the mixture was ice-incubated for 30min, frozen in liquid nitrogen for 5min at 37℃and then pre-incubated in 800. Mu.L LYEB liquid medium at 28℃and 200rpm for 3h, the liquid was plated on YEB (50. Mu.g/mL rifampin+50. Mu.g/mL kanamycin) solid medium and cultured in dark at 28℃for 2 days, and positive clones were selected for transformation of the Arabidopsis inflorescence.
(2) And (5) carrying out dip dyeing and seed harvesting on the arabidopsis inflorescences: positive monoclonal was inoculated into 50mLYEB (50. Mu.g/mL rifampicin+50. Mu.g/mL kanamycin) liquid medium, cultured for 36 hours, centrifuged at 5000rpm for 20 minutes, and then colonies were suspended with the infection solution (500 uL/L Silwet L-77+5% sucrose) until they were completely suspended. Directly soaking aerial parts of Arabidopsis thaliana in the suspension for 1min, then completely wrapping plants with a preservative film to preserve moisture, placing the plants back into a culture room for dark culture for 24 h, opening the preservative film, culturing under normal illumination, and harvesting when seeds are mature.
(3) Seed disinfection and sowing: the seeds are placed into a 5mL centrifuge tube, 50% Bass disinfectant is added, 0.1% Triton X-100 is added to shake for 15 minutes, then the seeds are washed with sterile water for 5 times in a super clean bench, then the seeds are directly poured onto sterilized filter paper, the seeds are dried (placed for 1 hour), the filter paper is lightly knocked to uniformly broadcast the dried seeds into a screening culture medium (1/2MS+20mg/L glufosinate) for screening and culturing for two weeks, positive seedlings are moved into soil, and the positive seedlings are covered with a transparent film for about 3 days to preserve moisture. And so on, screening to obtain T 2 The seeds of the generation are sown on a screening plate, positive seedlings with the survival rate of 100% are reserved, 7-8 leaves of the resistant seedlings are reserved, the arabidopsis leaf DNA is extracted by using a Norvezan DNA extraction kit (FastPure Plant DNA Isolation Mini Kit, DC 104), and genome DNA is used as a template, and Primer1 and Primer2 in the embodiment 1 are used as primers for amplification.
PCR reaction system: DNA (20 ng/. Mu.L) 2. Mu.L, primer1 (10. Mu. Mol. L) -1 )2μL，Primer2(10μmol·L -1 )2μL，10×Buffer(MgCl 2 free)2μL，dNTP(10mM)0.4μL，MgCl 2 (25mM)1.2μL，rTaq(5U/μL)0.4μL，ddH 2 O10. Mu.L, total volume 20. Mu.L.
Amplification reaction: 94 ℃ for 3min;94℃30s,55℃1min,72℃2min,35 cycles; and at 72℃for 5min.
The PCR products were electrophoresed on a 1% agarose gel to determine transgenic positive plants, and the results were shown in FIG. 3, using wild-type Arabidopsis thaliana (WT) as a negative control, and using the pCAMBIA1305-ZmLA1 positive plasmid obtained in example 2 as a positive control.
And (5) seed collection is carried out on the positive homozygous transgenic line obtained through identification, and the positive homozygous transgenic line is used for subsequent salt tolerance phenotype evaluation.
Example 4
Evaluation of salt tolerance of wild type and transgenic Arabidopsis plants
For wild type arabidopsis and pCAMBIA1305-ZmLA1 transgenic positive T2 generation transgenic seeds identified by PCR, respectively placing the transgenic seeds in a control (MS culture medium) and a salt treatment (100 mM NaCl+MS culture medium) to grow for 14 days, and the result is shown in figure 4, which shows that the growth of the transgenic arabidopsis for transforming the ZmLA1 gene is obviously superior to that of the wild type arabidopsis, and the salt tolerance is obviously improved.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.

Claims (9)

1. The zoysia japonica salt-tolerant gene ZmLA1 is characterized in that the nucleotide sequence of the salt-tolerant gene ZmLA1 is shown as SEQ ID NO. 1.
2. The zoysia japonica salt-tolerant protein is characterized in that the amino acid sequence of the salt-tolerant protein is shown as SEQ ID NO. 2.
3. A recombinant expression vector comprising the salt tolerance gene ZmLA1 of claim 1.
4. The recombinant expression vector of claim 3, wherein the backbone of the vector comprises pCAMBIA1305.
5. An expression cassette, transgenic cell line, recombinant bacterium or recombinant virus comprising the salt-tolerant gene ZmLA1 of claim 1.
6. Use of the salt tolerance gene ZmLA1 of claim 1, the salt tolerance protein of claim 2, the recombinant expression vector of any one of claims 3-4 or the expression cassette, transgenic cell line, recombinant bacterium or recombinant virus of claim 5 for increasing salt tolerance of a plant; the plant is Arabidopsis thaliana.
7. Use of the salt tolerant gene ZmLA1 of claim 1, the salt tolerant protein of claim 2, the recombinant expression vector of any one of claims 3-4 or the expression cassette, transgenic cell line, recombinant bacterium or recombinant virus of claim 5 in salt tolerant plant breeding; the plant is Arabidopsis thaliana.
8. A method of growing a salt tolerant transgenic plant comprising the steps of: introducing the salt-tolerant gene ZmLA1 of claim 1 into a plant to obtain a transgenic plant; the plant is Arabidopsis thaliana.
9. The method according to claim 8, wherein the method of introducing comprises the recombinant expression vector according to any one of claims 3-4 or the expression cassette, transgenic cell line, recombinant bacterium or recombinant virus according to claim 5.
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