CN116616172A - Full-automatic electron accelerator biological irradiation breeding mutagenesis device - Google Patents
Full-automatic electron accelerator biological irradiation breeding mutagenesis device Download PDFInfo
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- CN116616172A CN116616172A CN202310819848.6A CN202310819848A CN116616172A CN 116616172 A CN116616172 A CN 116616172A CN 202310819848 A CN202310819848 A CN 202310819848A CN 116616172 A CN116616172 A CN 116616172A
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- 230000001488 breeding effect Effects 0.000 title claims abstract description 35
- 238000009395 breeding Methods 0.000 title claims abstract description 34
- 238000002703 mutagenesis Methods 0.000 title claims abstract description 27
- 231100000350 mutagenesis Toxicity 0.000 title claims abstract description 27
- 230000007246 mechanism Effects 0.000 claims abstract description 65
- 238000001816 cooling Methods 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 238000012935 Averaging Methods 0.000 claims abstract description 6
- 230000005540 biological transmission Effects 0.000 claims description 31
- 210000001503 joint Anatomy 0.000 claims description 5
- 238000004378 air conditioning Methods 0.000 claims 1
- 230000035772 mutation Effects 0.000 abstract description 9
- 238000013461 design Methods 0.000 description 20
- 230000005855 radiation Effects 0.000 description 11
- 238000000034 method Methods 0.000 description 9
- 230000008569 process Effects 0.000 description 6
- 230000033001 locomotion Effects 0.000 description 5
- 230000036438 mutation frequency Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- 239000000941 radioactive substance Substances 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- 230000007723 transport mechanism Effects 0.000 description 2
- 230000001960 triggered effect Effects 0.000 description 2
- 230000005461 Bremsstrahlung Effects 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 229910017052 cobalt Inorganic materials 0.000 description 1
- 239000010941 cobalt Substances 0.000 description 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 238000010894 electron beam technology Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000010330 laser marking Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 238000009394 selective breeding Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/09—Apparatus for producing changes in chromosome number
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- G—PHYSICS
- G21—NUCLEAR PHYSICS; NUCLEAR ENGINEERING
- G21K—TECHNIQUES FOR HANDLING PARTICLES OR IONISING RADIATION NOT OTHERWISE PROVIDED FOR; IRRADIATION DEVICES; GAMMA RAY OR X-RAY MICROSCOPES
- G21K5/00—Irradiation devices
- G21K5/10—Irradiation devices with provision for relative movement of beam source and object to be irradiated
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- Life Sciences & Earth Sciences (AREA)
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- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
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- High Energy & Nuclear Physics (AREA)
- Radiation-Therapy Devices (AREA)
Abstract
The invention belongs to the technical field of irradiation breeding mutagenesis devices, and provides a full-automatic electron accelerator biological irradiation breeding mutagenesis device. The invention comprises the following steps: a fixed box; the electronic accelerator is arranged in the fixed box and comprises a power supply modulator, an accelerator machine head and a water cooling unit; the power modulator is electrically connected with the accelerator handpiece; the collimation-averaging device is arranged in the fixed box and is positioned below the accelerating tube; the collimation-equalizer is used for correcting the non-uniformity of the X-rays; the multi-leaf grating system is arranged below the collimation-averaging device; the multi-leaf grating system is used for conducting space division guiding on the irradiation dose corrected by the collimation-equalizer; the conveying mechanism comprises a conveying line and a tray; the device can solve the problem of adjustable and controllable mutation breeding energy.
Description
Technical Field
The invention relates to the technical field of irradiation breeding mutagenesis devices, in particular to a full-automatic electron accelerator biological irradiation breeding mutagenesis device.
Background
Mutation breeding refers to a breeding method which uses physical and chemical factors to induce genetic characteristics of animals and plants to change, then selects single plants/individuals meeting certain requirements of people from mutation groups, and further breeds new varieties or germplasm. It is a modern breeding technology developed after selective breeding and cross breeding.
At present, the problem of insufficient seed gene samples is solved in the field of agricultural breeding, and mutation breeding is the fastest technical means for solving the problem.
The current common mutagenesis method comprises X-ray irradiation and ray irradiation; generally, cobalt sources are used for generating rays, neutrons or other high-energy particle mutagenesis, space breeding, chemical mutagenesis and the like. However, these methods often have the defects of difficulty in precisely controlling the radiation dose, limited load, requirement of a special site for radioactive source substances, great environmental hazard, great implementation difficulty, high cost and the like.
Therefore, a device which can well solve the problem of adjustable and controllable energy of mutation breeding is needed. The problems of high seed mortality and insufficient mutation frequency caused by uncontrollable irradiation dose can be effectively avoided. And the radiation is generated without a radioactive substance source, so that the radiation is safe and reliable to use, and the influence on the environment and personnel is safe and controllable.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a full-automatic electron accelerator biological irradiation breeding mutagenesis device, which aims to solve the problem of adjustable and controllable mutagenesis breeding energy.
The invention provides a full-automatic electron accelerator biological irradiation breeding mutagenesis device, which comprises: a fixed box; the electronic accelerator is arranged in the fixed box and comprises a power supply modulator, an accelerator machine head and a water cooling unit; the power modulator is electrically connected with the accelerator handpiece; the water cooling unit is arranged on one side of the accelerator machine head; the power supply modulator is used for converting industrial alternating current into electrically controlled high-voltage pulse current; the accelerator handpiece comprises an accelerator and an accelerating tube; the accelerator is electrically connected with the power modulator; the water cooling unit comprises a water cooling sleeve, and the water cooling sleeve is communicated with an external water source; the accelerating tube is arranged in the water-cooling sleeve; the collimation-averaging device is arranged in the fixed box and is positioned below the accelerating tube; the collimation-equalizer is used for correcting the non-uniformity of the X-rays; the multi-leaf grating system is arranged below the collimation-averaging device; the multi-leaf grating system is used for conducting space division guiding on the irradiation dose corrected by the collimation-equalizer; the conveying mechanism comprises a conveying line and a tray; the trays are arranged on the conveying line at least at one position and are uniformly distributed along the direction of the conveying line; the conveying line is in a shape like a Chinese character 'Hui'; the tray is used for placing seeds to be bred and is positioned below the multi-leaf grating system; the fixed box is arranged on the transmission mechanism and is positioned above the conveying line.
Further, the conveying mechanism further comprises a lifting and transverse moving mechanism, and the lifting and transverse moving mechanism is arranged on the conveying line. In practical application, in the design, the lifting and transversely moving mechanism is used for turning the tray on the conveying line so as to realize the circulating rotation of the tray on the conveying line and realize multiple irradiation.
Further, the jacking and transversely moving mechanism comprises a base and a first cylinder; one end of the first cylinder is fixedly arranged in the conveying line; the other end of the first cylinder is fixedly arranged on the lower surface of the base. In practical application, the design can lift the base and the components mounted on the base integrally, so that the tray is convenient to turn.
Further, the jacking and transversely moving mechanism further comprises a transmission belt and a motor; the motor is installed on the base, the transmission belt is installed on two sides of the base, and the transmission belt is in transmission connection with the motor. In practical application, the transmission belt has two parts and is horizontally symmetrical in the design; unbalanced shaking of the tray during steering can be greatly reduced. Meanwhile, in the design, a motor is adopted to drive a transmission shaft, a transmission wheel and the like to drive, and transmission belts at two ends rotate; because the drive transmission belt is of conventional design, it will not be described in detail herein; in this way, the rotating drive pulley will cause the pallet to turn, since in design the drive direction of the drive pulley is perpendicular to the direction of movement of the conveyor line; thus, under the jacking of the first cylinder, the conveying belt is abutted with the tray; the conveyor belt then rotates the tray to be diverted.
Further, the transmission mechanism further comprises a stopping mechanism, and the stopping mechanism is arranged on the conveying line and is positioned at the rear of the jacking and transversely moving mechanism; the stop mechanism comprises a fixed plate, a fixed frame and a second cylinder; the fixing plate is arranged in the conveying line and is provided with a fixing through groove and a fixing hole; the fixing holes are formed in four places, and the second air cylinder is fixedly arranged at the fixing through groove through the fixing holes; the fixing frame is fixedly arranged on the fixing through groove. In practice, the purpose of this design is to achieve a stop for the pallet to continue moving on the conveyor line, and wherein the fixing plate can effectively fix the design.
Further, the stop mechanism further comprises a rotary abutting joint; the fixing frame is provided with a rotating hole, the rotating butt joint is rotatably arranged on the rotating hole, and the rotating butt joint is connected with an output shaft of the second air cylinder. In practical application, rotate the butt joint and realize upwards overturning under the connection of second cylinder, so, after it upwards overturns, just can realize the butt with the tray, very big satisfying work needs.
Further, the device also comprises a marking machine, wherein the marking machine is used for marking; the method is mainly used for determining the batch of the irradiated samples and tracing the parameters of the irradiated samples.
According to the technical scheme, the full-automatic electron accelerator biological irradiation breeding mutagenesis device has the beneficial effects that:
(1) In practical application, the adopted electron accelerator is a linear electron accelerator, so that the problem of adjustable and controllable mutation breeding energy can be well solved, and the problems of over-high seed mortality and insufficient mutation frequency caused by uncontrollable irradiation dose can be avoided;
(2) The radiation is generated without a radioactive substance source, so that the radiation is safe and reliable to use, and the influence on the environment and personnel is safe and controllable;
(3) Meanwhile, an automatic transmission mechanism is adopted, so that manual operation is greatly reduced, and efficiency can be effectively improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention, the drawings that are used in the description of the embodiments or the prior art will be briefly described below. Throughout the drawings, the elements or portions are not necessarily drawn to actual scale.
FIG. 1 is a front view of a full-automatic electron accelerator biological irradiation breeding mutagenesis device according to an embodiment of the invention;
FIG. 2 is a schematic perspective view of the transport mechanism shown in FIG. 1;
FIG. 3 is an enlarged view of a portion of the transport mechanism shown in FIG. 2;
FIG. 4 is a schematic left view of a lifting and traversing mechanism in the present invention;
FIG. 5 is a schematic top view of a lifting and traversing mechanism according to the present invention;
FIG. 6 is a schematic diagram of a top stop mechanism in a front view of the present invention;
FIG. 7 is a schematic top view of a fixing plate in the lifting and traversing mechanism of the present invention;
fig. 8 is a schematic diagram of a marking machine in the present invention.
The drawing marks:
the electronic accelerator comprises a fixed box 1, an electronic accelerator 2, a power modulator 21, an accelerator head 22, a water cooling unit 23, an accelerator 221, an accelerating tube 222, a water cooling sleeve 231, a collimation-leveling device 3, a multi-blade grating system 4, a transmission mechanism 5, a conveying line 51, a tray 52, a jacking and traversing mechanism 53, a base 531, a first cylinder 532, a transmission belt 533, a motor 534, a stopping mechanism 54, a fixed plate 541, a fixed frame 542, a second cylinder 543, a rotary abutting joint 544, a fixed through groove 101, a fixed hole 102, a microwave power source 103, a waveguide device 104, an electronic gun 105 and a marking machine 6.
Detailed Description
Embodiments of the technical scheme of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for more clearly illustrating the technical aspects of the present invention, and thus are merely examples, and are not intended to limit the scope of the present invention.
An example is substantially as shown in figures 1 to 8:
example 1:
as shown in fig. 1-8, the full-automatic electron accelerator biological irradiation breeding mutagenesis device provided by the embodiment can solve the problem that the mutagenesis breeding energy is adjustable and controllable.
The invention provides a full-automatic electron accelerator biological irradiation breeding mutagenesis device, which comprises: a fixed box 1; an electron accelerator 2, the electron accelerator 2 is installed in the fixed box 1, and the electron accelerator 2 comprises a power supply modulator 21, an accelerator head 22 and a water cooling unit 23; the power modulator 21 is electrically connected with the accelerator handpiece 22; the water cooling unit 23 is installed at one side of the accelerator head 22; the power modulator 21 is used for converting industrial alternating current into electrically controlled high-voltage pulse current; the accelerator handpiece 22 includes an accelerator 221 and an acceleration tube 222; the accelerator 221 is electrically connected to the power modulator 21; the water cooling unit 23 comprises a water cooling sleeve 231, and the water cooling sleeve 231 is communicated with an external water source; the accelerating tube 222 is installed in the water-cooling jacket 231; a collimator-equalizer disposed within the stationary box 1, the collimator-equalizer being located below the accelerating tube 222; the collimation-equalizer is used for correcting the non-uniformity of the X-rays; a multi-leaf collimator system 4, the multi-leaf collimator system 4 being arranged below the collimator-equalizer; the multi-leaf grating system 4 is used for conducting space division guiding on the irradiation dose corrected by the collimation-equalizer; and a conveying mechanism 5, the conveying mechanism 5 comprising a conveying line 51 and a tray 52; the trays 52 are arranged on the conveying line 51 at least one place and are uniformly distributed along the direction of the conveying line 51; the conveying line 51 is in a shape of a Chinese character 'hui'; the tray 52 is used for being placed on seeds to be bred and is positioned below the multi-leaf grating system 4; the fixed box 1 is disposed on the conveying mechanism 5 and above the conveying line 51. In practical application, the adopted electron accelerator 2 is the linear electron accelerator 2, so that the problems of adjustable and controllable mutation breeding energy and over-high seed mortality and insufficient mutation frequency caused by uncontrollable irradiation dose can be well solved. And the radiation is generated without a radioactive substance source, so that the radiation is safe and reliable to use, and the influence on the environment and personnel is safe and controllable. Meanwhile, the automatic transmission mechanism 5 is adopted, so that manual operation is greatly reduced, and the efficiency can be effectively improved.
Meanwhile, the electron accelerator 2 mainly comprises a power supply modulator 21, an accelerator head 22 and a water cooling unit 23. The power modulator 21 provides high-voltage and control signals for the accelerator head 22, and the water cooling unit 23 provides cooling for an accelerating tube 222 of the accelerator head 22 to ensure that the accelerator head 22 works in a stable state.
The power modulator 21 converts the industrial alternating current into high voltage pulses that are electrically controlled by an internal ac-dc-ac rectification inversion process to power the microwave power source 103 in the accelerator handpiece 22. The microwave power source 103 generates a high-power microwave signal, the microwave power feeds electrons into the accelerating tube 222 through the waveguide device 104, and after the microwaves generated by the microwave power source are fed into the accelerating tube 222, a forward wave and a backward wave are formed in the accelerating tube 222, and the forward wave and the backward wave are overlapped to form a standing wave field. At the same time, the electron gun 105 controls the electron output, the electrons interact with the axial standing wave electric field in the accelerating tube 222, interact with the radio frequency accelerating electric field, and absorb energy therefrom, so that the energy of the electrons is increased. Electrons are continuously accelerated in the standing wave field and when the final energy reaches the target energy, the electrons generate continuous spectrum X-rays in the accelerator head 22 accelerating terminal accelerator tube 222.
Limited by the bremsstrahlung principle, the number and energy of X-ray photons in different directions of radiation after electron beam targeting are different, i.e. the dose rate exhibits directional non-uniformity. The radiation exiting the accelerator handpiece 22 has different dose rates over different angular ranges, and the irradiation dose can directly affect the breeding effect. The collimation-leveling device 3 is mainly used for correcting the non-uniformity of the X-rays and is used for improving the influence of inaccurate irradiation dose on the breeding effect. And the whole control operation is carried out by adopting an electric control mode, wherein the PLC is used as a central control unit, the PLC is remotely transmitted by upper computer set software to control the work of the PLC, and other auxiliary electric elements realize signal interaction with the PLC through a dry node. In order to determine the batch of the irradiation samples and trace the irradiation sample parameters, a marking recognition machine consisting of a marking machine and a code scanner is also adopted, and the marking machine is mainly used for marking the tray 52 and recording the irradiation sample information and the irradiation parameters. The code scanner is used for identifying marks established on the tray 52 by the marking machine; this design is conventional and will not be described in detail herein.
In this embodiment, the conveying mechanism 5 further includes a lifting and traversing mechanism 53, and the lifting and traversing mechanism 53 is mounted on the conveying line 51. In practical use, in this design, the lifting and traversing mechanism 53 is used to turn the tray 52 on the conveying line 51, so as to implement the cyclic rotation of the tray 52 on the conveying line 51, so that multiple irradiation can be performed.
In this embodiment, the lifting and traversing mechanism 53 includes a base 531 and a first cylinder 532; one end of the first cylinder 532 is fixedly installed in the conveying line 51; the other end of the first cylinder 532 is fixedly installed on the lower surface of the base 531. In practice, the design can lift the base 531 and the components mounted on the base 531, thereby facilitating the turning of the tray 52.
In this embodiment, the lifting and traversing mechanism 53 further includes a transmission belt 533 and a motor 534; the motor 534 is mounted on the base 531, the transmission belt 533 is mounted on two sides of the base 531, and the transmission belt 533 is in transmission connection with the motor 534. In practical application, the transmission belt 533 in the design has two positions and is horizontally symmetrical; unbalanced rocking of the tray 52 at the time of steering is greatly reduced. Meanwhile, in the design, a motor 534 drives a transmission shaft, a transmission wheel and the like to drive, and the transmission belts 533 at the two ends rotate; because the drive transmission belt 533 in this design is a conventional design, it will not be described in detail here; the rotating drive pulley will cause the pallet 52 to turn, because in design the drive direction of the drive pulley is perpendicular to the direction of movement of the conveyor line 51; thus, the conveyor belt 533 will contact the tray 52 by the pushing up of the first cylinder 532; the transfer belt 533 then rotates the tray 52 to be turned.
In this embodiment, the conveying mechanism 5 further includes a stopping mechanism 54, and the stopping mechanism 54 is mounted on the conveying line 51 and is located at the rear of the lifting and traversing mechanism 53; the stop mechanism 54 comprises a fixed plate 541, a fixed frame 542 and a second cylinder 543; the fixing plate 541 is installed in the conveying line 51, and the fixing plate 541 has a fixing through slot 101 and a fixing hole 102; the fixing holes 102 are arranged everywhere, and the second air cylinders 543 are fixedly arranged at the fixing through grooves 101 through the fixing holes 102; the fixing frame 542 is fixedly installed on the fixing through groove 101. In practice, the purpose of this design is to achieve a blocking of the further movement of the pallet 52 on the conveyor line 51, and wherein the fixing plate 541 is able to effectively fix the design.
In this embodiment, the stop mechanism 54 further includes a rotational abutment 544; the fixing frame 542 has a rotation hole, the rotation abutting joint 544 is rotatably mounted on the rotation hole, and the rotation abutting joint 544 is connected with the output shaft of the second cylinder 543. In practical application, the rotary abutting joint 544 is connected with the second air cylinder 543 to realize upward overturning, so that the rotary abutting joint can abut against the tray 52 after upward overturning, and the requirement of work is greatly met.
In this embodiment, the device further comprises a marking machine 6, wherein the marking machine 6 is used for marking.
And the microwave power source 103 may employ other devices or mechanisms including, but not limited to, magnetrons, klystrons, etc. that implement the embodiments of the invention, while remaining within the scope of the invention.
The electron accelerator 2 can adopt other devices or mechanisms which can realize the embodiment of the invention, such as a linear accelerator, a cyclotron and the like, and the devices or mechanisms are all within the protection scope of the invention.
The microwave power source 103 can be any other device or mechanism capable of realizing the embodiments of the present invention, including but not limited to a magnetron, klystron, etc., and is within the scope of the present invention. The related conveyor line 51 can adopt other devices or mechanisms including but not limited to a double-speed chain conveyor line 51, a belt conveyor line 51 and the like which can realize the embodiment of the invention, and the devices and the mechanisms are all within the protection scope of the invention.
The marking machine 6 may employ other devices or mechanisms including, but not limited to, a laser marking machine, a code jet machine, an optical fiber marking machine, an ink jet marking machine 6, etc. that implement the embodiments of the present invention, and are within the scope of the present invention.
In actual operation;
the automatic irradiation flow is as follows:
s101: the user sets irradiation parameters through upper computer set software.
S102: the user places the irradiation samples into trays 52 on the conveyor line 51 according to the irradiation parameter setting sequence;
s103: the user clicks a software start button to send a start command to start the motion of the conveying line 51, and the control flow starts;
s104: after the irradiation sample reaches the stop mechanism 54, the sensor is triggered and feeds back an in-place signal to the electric control subsystem, the PLC controls the stop mechanism 54 to act, and the irradiation sample is accurately parked to an irradiation position;
s105: after the irradiation sample is in place, the PLC automatically executes the irradiation process according to the irradiation parameters which are set by a user through upper computer set software, and the system enters an irradiation state;
s106: the irradiation state is started, the electric control subsystem controls the power modulator 21 to output high-voltage pulse, the microwave power source 103 in the machine head 22 of the accelerator 221 is powered on, the microwave power source 103 generates high-power microwave signals, electrons are fed into the accelerating tube 222 to accelerate the electrons, and when the energy reaches the target energy set by a user in the upper computer state software, the electrons generate continuous spectrum X rays at the accelerating terminal of the machine head 22 of the accelerator 221. The collimation-leveling device subsystem corrects the non-uniformity of the X-rays, the irradiation dose corrected by the collimation-leveling device subsystem is fed into the multi-leaf grating subsystem, and the multi-leaf grating subsystem automatically controls each leaf in the multi-leaf grating subsystem to independently move according to irradiation parameters set by a user through upper computer unit software, so that an irradiation result is converted into a batch result of an irradiation mutagenesis experiment with multiple radiation doses in parallel.
S107: after the irradiation is finished, the electric control subsystem controls the stop mechanism 54 to stop moving, the conveying line 51 conveys the tray 52 to a finishing station, the marking machine 6 marks the tray 52, the single automatic irradiation process is finished, and the electric control subsystem continues to execute the next automatic irradiation process until the number of irradiation samples set by a user is reached.
The manual irradiation mode is mainly used for debugging devices or single-piece small-batch experiments, specifically, a user only places one irradiation sample on the conveying line 51, irradiation parameters are set through upper computer set software, and the device is operated after the setting is completed. After the device is started to run, the electric control subsystem executes the irradiation flow according to the user instruction single thread.
And meanwhile, the manual irradiation process comprises the following steps:
s201: the user sets irradiation parameters through upper computer set software.
S202: the user places the irradiated sample into a tray 52 on the conveyor line 51;
s203: the user clicks a software start button to send a manual command to start the movement of the conveying line 51, and the control flow starts;
s204: after the irradiation sample reaches the stop mechanism 54, the sensor is triggered and feeds back an in-place signal to the electric control subsystem, the PLC controls the stop mechanism 54 to act, and the irradiation sample is accurately parked to an irradiation position;
s205: after the irradiation sample is in place, a user clicks a software irradiation button, an irradiation command is sent, the PLC executes an irradiation process according to irradiation parameters which are set by the user through upper computer set software, and the system enters an irradiation state;
s206: the irradiation state is started, the electric control subsystem controls the power modulator 21 to output high-voltage pulse, the microwave power source 103 in the machine head 22 of the accelerator 221 is powered on, the microwave power source 103 generates high-power microwave signals, electrons are fed into the accelerating tube 222 to accelerate the electrons, and when the energy reaches the target energy set by a user in the upper computer state software, the electrons generate continuous spectrum X rays at the accelerating terminal of the machine head 22 of the accelerator 221. The collimation-leveling device subsystem corrects the non-uniformity of the X-rays, the irradiation dose corrected by the collimation-leveling device subsystem is fed into the multi-leaf grating subsystem, and the multi-leaf grating subsystem automatically controls each leaf in the multi-leaf grating subsystem to independently move according to irradiation parameters set by a user through upper computer unit software, so that an irradiation result is converted into a batch result of an irradiation mutagenesis experiment with multiple radiation doses in parallel.
S207: after the irradiation is finished, the user clicks a software end button to send an end command, the electric control subsystem controls the stop mechanism 54 to stop acting, the conveying line 51 conveys the tray 52 to a finishing station, the marking machine 6 marks the tray 52, and the manual irradiation flow is finished.
In conclusion, the full-automatic electron accelerator biological irradiation breeding mutagenesis device is reasonable in design, simple to operate and capable of greatly reducing manual operation; meanwhile, in the design, the adopted linear electron accelerator 2 can well solve the problem of adjustable and controllable mutation breeding energy, and can avoid the problems of too high seed mortality and insufficient mutation frequency caused by uncontrollable irradiation dose; the device can effectively solve the problem of adjustable and controllable mutation breeding energy, so the device is suitable for industrial popularization.
The above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention, and are intended to be included within the scope of the appended claims and description.
Claims (7)
1. A full-automatic electron accelerator biological irradiation breeding mutagenesis device is characterized in that the device comprises:
a fixed box;
the electronic accelerator is arranged in the fixed box and comprises a power supply modulator, an accelerator machine head and a water cooling unit; the power modulator is electrically connected with the accelerator handpiece; the water cooling unit is arranged on one side of the accelerator machine head; the power supply modulator is used for converting industrial alternating current into high-voltage pulse current which is electrically controlled; the accelerator handpiece comprises an accelerator and an accelerating tube; the accelerator is electrically connected with the power modulator; the water cooling unit comprises a water cooling sleeve, and the water cooling sleeve is communicated with an external water source; the accelerating tube is arranged in the water-cooling sleeve;
the collimation-averaging device is arranged in the fixed box and is positioned below the accelerating tube; the collimation-equalizer is used for correcting the non-uniformity of the X-rays;
the multi-leaf grating system is arranged below the collimation-averaging device; the multi-leaf grating system is used for conducting space division guiding on the irradiation dose corrected by the collimation-equalizer; a kind of electronic device with high-pressure air-conditioning system
The conveying mechanism comprises a conveying line and a tray; the trays are arranged on the conveying line at least at one position and are uniformly distributed along the direction of the conveying line; the conveying line is in a shape like a Chinese character 'Hui';
the tray is used for placing seeds to be bred and is positioned below the multi-leaf grating system;
the fixed box is arranged on the transmission mechanism and is positioned above the conveying line.
2. The full-automatic electron accelerator biological irradiation breeding mutagenesis device according to claim 1, wherein the transmission mechanism further comprises a lifting and traversing mechanism, and the lifting and traversing mechanism is mounted on the conveying line.
3. The full-automatic electron accelerator biological irradiation breeding mutagenesis device according to claim 2, wherein the jacking and traversing mechanism comprises a base and a first cylinder; one end of the first cylinder is fixedly arranged in the conveying line; the other end of the first cylinder is fixedly arranged on the lower surface of the base.
4. The full-automatic electron accelerator biological irradiation breeding mutagenesis device according to claim 3, wherein the lifting and traversing mechanism further comprises a transmission belt and a motor; the motor is installed on the base, the transmission belt is installed on two sides of the base, and the transmission belt is in transmission connection with the motor.
5. The full-automatic electron accelerator biological irradiation breeding mutagenesis device according to claim 4, wherein the transmission mechanism further comprises a stop mechanism, and the stop mechanism is installed on the conveying line and is positioned behind the jacking and traversing mechanism; the stop mechanism comprises a fixed plate, a fixed frame and a second cylinder; the fixing plate is arranged in the conveying line and is provided with a fixing through groove and a fixing hole; the fixing holes are formed in four places, and the second air cylinder is fixedly arranged at the fixing through groove through the fixing holes; the fixing frame is fixedly arranged on the fixing through groove.
6. The full-automatic electron accelerator biological irradiation breeding mutagenesis device according to claim 5, wherein the stopping mechanism further comprises a rotary abutting joint; the fixing frame is provided with a rotating hole, the rotating butt joint is rotatably arranged on the rotating hole, and the rotating butt joint is connected with an output shaft of the second air cylinder.
7. The full-automatic electron accelerator biological irradiation breeding mutagenesis device according to claim 1, further comprising a marking machine for marking.
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CN202310819848.6A CN116616172A (en) | 2023-07-05 | 2023-07-05 | Full-automatic electron accelerator biological irradiation breeding mutagenesis device |
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CN202310819848.6A CN116616172A (en) | 2023-07-05 | 2023-07-05 | Full-automatic electron accelerator biological irradiation breeding mutagenesis device |
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CN116762693A (en) * | 2023-08-24 | 2023-09-19 | 三亚鸣遥种质创新科技有限公司 | Germplasm resource irradiation mutagenesis equipment |
CN116762693B (en) * | 2023-08-24 | 2023-10-27 | 三亚鸣遥种质创新科技有限公司 | Germplasm resource irradiation mutagenesis equipment |
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