CN1164732C

CN1164732C - Multi-division centrifugal tube

Info

Publication number: CN1164732C
Application number: CNB011283572A
Authority: CN
Inventors: 张建国; 杰; 皇甫留杰; 付坚
Original assignee: SHENZHEN SANJIU GENE ENGINEERING Co Ltd
Current assignee: Shenzhen Sanjiu Gene Engineering Co., Ltd.
Priority date: 2001-08-20
Filing date: 2001-08-20
Publication date: 2004-09-01
Anticipated expiration: 2021-08-20
Also published as: CN1334338A

Abstract

The present invention discloses a centrifugal pipe for a biological engineering gene field, and separations are arranged in a pipe cavity. The centrifugal pipe is composed of a pipe body, a pipe cover, a seal plate and a protection film. The separations vertically communicated with the pipe cavity are arranged in the pipe cavity, the pipe cavity is divided into a plurality of areas which are not mutually communicated by the separations, and the inner part of the pipe cavity is divided into 2 to 6 areas. The centrifugal pipe has multiple functions and equal conditions, and a plurality of PCR amplification reactions can be completed under the same background in the same pipe. The centrifugal pipe is also suitable for the storage, the transportation and the reactions of a plurality of micro metering systems under the same condition. The centrifugal pipe with the multiple functions enhances efficiency, simplifies an amplification technology and effectively prevents pollution.

Description

Multi-division centrifugal tube

One, technical field:

The present invention relates to a kind of reaction vessel that are used for biotechnology gene field, especially a kind of reaction centrifuge tube.

Two, background technology:

In the prior art, subregion not in the employed common centrifuge tube tube chamber in the genetically engineered field, can only put into one group of sample in the every common centrifuge tube reacts or/and separate, many group samples must be put into a plurality of centrifuge tubes and react or/and separate, this is easy to generate especially and pollutes and pollution, mutual interference mutually mutually for the test of little metering of the molecular level in the gene field.And, when requiring many group samples under same background, to finish, for example when primer amplification reacted in a plurality of centrifuge tubes, its temperature of reaction, time, test conditions etc. all were difficult to control in full accordly many group PCR (Polymerase chain reaction, polymerase chain reaction).

Three, summary of the invention:

The objective of the invention is limitation and deficiency at common high core barrel in the prior art, the centrifuge tube that is provided with one or more separations in a kind of tube chamber is provided, make many group biochemical reactions can be in equality of temperature, simultaneously, finish with under the condition, and will pollute may be reduced to minimum.

For solving above-mentioned task; the solution that the present invention adopts is: be provided with the partition that tube chamber is divided into the vertical perforation tube chamber in several mutual disconnected zones in the tube chamber of common centrifuge tube body; sealing plate is round pie; be placed on the intraluminal partition; the protective membrane that be thin discs shape consistent with the sealing plate diameter is placed on the sealing plate; when body that the employing manner connects and Guan Gai mutually combined, boss of pipe lid or setting in it just in time compressed sealing plate.

Subregion is 2～6 in the tube chamber; have the corresponding to manhole of number of regions and position that several and tube chamber are separated on the sealing plate; sealing plate diameter consistent with body internal diameter suitable for reading; sealing plate thickness and body pipe lid in conjunction with the time Guan Gai lower edge or its boss lower edge and the distance cut off between the upper edge consistent, protective membrane and sealing plate also can adopt hot pressing or adhesive bond.

Body pipe lid can adopt multiple movable connection method to combine; Can with the lower end of managing cover cavity the screw thread that cooperatively interacts be set in body outer suitable for reading combines; Pipe can cover external diameter and body internal diameter suitable for reading is designed to same size, pipe covers direct pressing goes into body combination suitable for reading and chimeric; Can outside tube chamber top and pipe lid the screw thread that cooperatively interacts be set combines; Also can adopt elastic annular bayonet socket mode to combine.

Body, Guan Gai, sealing plate are made by the transparent polypropylene of white, and protective membrane is made by the transparent high-pressure polyethylene of white, also all can make of transparent, high pressure resistant, free of contamination other material.

The present invention is provided with partition in tube chamber, solved the limitation of common centrifuge tube dexterously, make one tube multipurpose, the condition equalization, can be in pipe with finishing many group pcr amplification reactions under the background, to multiple system can be in equality of temperature, simultaneously, finish biochemical reaction with under the condition, reduce multiple reacting phase mutual interference.Sealing plate setting, play and concentrate sealing, effective antipollution effect is applicable to that also little metering multi-primers is with preservation under the condition, transportation and pcr amplification.One tube multipurpose has improved efficient, easy amplification technology, and saved a large amount of common centrifuge tubes.

Four, description of drawings:

Accompanying drawing 1 is with outside screw bonded multi-division centrifugal tube assembling synoptic diagram;

Accompanying drawing 2 is with the chimeric bonded multi-division centrifugal tube of ring-type bayonet socket body pipe lid synoptic diagram;

Accompanying drawing 3 is vertical views of accompanying drawing 2;

Accompanying drawing 4 is triple division centrifugal tube body synoptic diagram;

Accompanying drawing 5 is vertical views of accompanying drawing 4;

Accompanying drawing 6 is outside screw connecting pipe lid synoptic diagram;

Accompanying drawing 7 is sealing plate schematic top plan view;

Accompanying drawing 8 is that internal thread is in conjunction with multi-division centrifugal tube body synoptic diagram;

Accompanying drawing 9 is internal thread connecting pipe lid synoptic diagram.

Mark division in the accompanying drawing is as follows: the 1-----body; 2----manages lid; The 3-----sealing plate; The 4-----protective membrane; 5-----cuts off; The 6----boss; The 7-----circular hole; 8-----connects slice; The 9-----ring-shaped step; The 10----pipe covers pressing place.

Five, embodiment:

Embodiment 1, as shown in Figure 1, body 1 and pipe cover 2 by the screw thread combination, cut off 5 inner chambers and be divided into three mutual disconnected zones body 1, it is lower slightly than the upper edge of body 1 up and down to cut off 5 perforation tube chambers, sealing plate 3 diameters are consistent with body internal diameter suitable for reading, the thickness of sealing plate 3 and body 1 pipe cover 2 in conjunction with the time pipe cover boss 6 lower edges and the distance cut off between 5 upper edges consistent.When using multi-division centrifugal tube of the present invention; after placing the multiple reaction mixture of little metering (comprising the PCR reaction system) in a plurality of zones respectively; sealing plate 3 is pressed into body 1 top to be placed on the partition 5; protective membrane 4 diameters are consistent with the diameter of sealing plate 3; be positioned on the sealing plate 3; lid upper tube cap 2, threaded together, pipe builds the boss 6 that is provided with on 2 inner chambers and under the control of threaded adjusting structure sealing plate 3 is compressed.After reaction is finished, open pipe and cover 2, the rifle point of will sampling stabs out protective membrane 4 respectively and enters each zone, body inside and sample respectively.

Embodiment 2; as accompanying drawing 2; shown in 3; the body 1 of multi-division centrifugal tube; it is with the chimeric mode bonded of ring-type bayonet socket that pipe covers 2; body 1 pipe has a slice 8 to be connected between covering 2; cut off 5 body 1 inner chamber is divided into 2 zones; it is lower slightly than body 1 upper edge up and down to cut off 5 perforation tube chambers; with the tube chamber that cuts off on the same horizontal plane in 5 upper edges in be provided with a ring-shaped step 9; it is consistent that the lumen diameter of ring-shaped step more than 9 covers 2 pressing place 10 external diameters with pipe; to manage cover 2 direct pressings go into that body 1 is suitable for reading can chimeric combination; the diameter of sealing plate 3 is consistent with body 1 internal diameter suitable for reading; protective membrane 4 diameters are consistent with sealing plate 3; place on the sealing plate 3; the thickness of sealing plate 3 and pipe covers that the pipe of 2 pressings when going into body covers 2 lower edges and the distance cut off between 5 upper edges is consistent, and pipe cover 2 and manage during body 1 pressing and cover 2 lower edges and just in time sealing plate 3 is compressed.

Embodiment 3, as shown in accompanying drawing 8,9, the body 1 of multi-division centrifugal tube, pipe cover 2 modes with internal thread and combine, cut off 5 body 1 is divided into three zones, it is low than mouth of pipe internal thread step to cut off 5 upper edges, the boss 6 that cover on 2 of pipe stretches to below the screw thread, when body 1 manage cover 2 in conjunction with the time, boss 6 just in time compresses sealing plate 3.

Claims

1, a kind of multi-division centrifugal tube; mainly form by body, pipe lid; it is characterized in that: in the tube chamber of common centrifuge tube body (1), be provided with the partition (5) that tube chamber is divided into the vertical perforation tube chamber in several mutual disconnected zones; sealing plate (3) is round pie; be placed on the intraluminal partition; the protective membrane that be thin discs shape (4) consistent with the sealing plate diameter is placed on the sealing plate; the body (1) and the pipe that adopt manner to connect cover (2) when mutually combining, and pipe lid (2) or the boss (6) that is provided with in it just in time compress sealing plate (3).

2, multi-division centrifugal tube according to claim 1; it is characterized in that: subregion is 2～6 in the tube chamber; have the corresponding to manhole of number of regions and position (7) that several and tube chamber are separated on the sealing plate (3); the diameter of sealing plate (3) is consistent with body (1) internal diameter suitable for reading; sealing plate thickness and body, pipe lid in conjunction with the time Guan Gai lower edge or its boss lower edge and the distance cut off between the upper edge consistent, protective membrane (4) also can adopt hot pressing or adhesive bond with sealing plate (3).

3, multi-division centrifugal tube according to claim 1 and 2 is characterized in that: body (1), pipe lid (2) can adopt movable connection methods such as outside screw, internal thread, ring-type bayonet socket are chimeric, elastic annular bayonet socket to combine.

4, according to the described multi-division centrifugal tube of arbitrary claim in the claim 1～3; it is characterized in that: body, Guan Gai, sealing plate are made by the transparent polypropylene of white; protective membrane is made by the transparent high-pressure polyethylene of white, also all can make of transparent, high pressure resistant, free of contamination other material.