CN116351490A - Single-electrode card micro-fluidic chip for multi-parameter biochemical molecule detection - Google Patents
Single-electrode card micro-fluidic chip for multi-parameter biochemical molecule detection Download PDFInfo
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Abstract
The invention provides a single-electrode card micro-fluidic chip for multi-parameter biochemical molecule detection, which particularly comprises a chip main body, a sample inlet arranged on the chip main body, a sample inlet pipeline system and a calibration liquid bag which are positioned on the front surface of the chip main body, and an electrode assembly area and a liquid guiding-out system which are positioned on the back surface of the chip main body. The sample injection pipeline system comprises a magnetic bead mixing area, a mixing pipeline and a miniature check valve which are sequentially communicated with a sample injection port, the electrode assembly area is connected with the sample injection pipeline system through a sample injection liquid channel buried in the chip main body, the sample injection liquid channel is communicated with the miniature check valve, at least one calibration liquid channel extends out through the miniature check valve to be connected with a calibration liquid package, and a flow guide valve is arranged between the calibration liquid channel and the calibration liquid package. The invention can reduce the cost and processing difficulty of the chip, improve the detection speed, sensitivity and accuracy while ensuring the simultaneous detection of multiple parameters, and realize the simultaneous detection of multiple indexes in the biochemical electrolyte.
Description
Technical Field
The invention belongs to the field of biosensors, and relates to a single-electrode card micro-fluidic chip for multi-parameter biochemical molecule detection.
Background
The current most novel POCT chemical sensing instrument product can realize that a plurality of samples are detected simultaneously, so that the detection time is greatly saved, and meanwhile, the instant detection is faster. POCT devices are mostly realized by using a biosensor, and the biological enzyme molecules are immobilized on a solid phase interface of a micro-analysis device, and after the analyte is specifically identified, the detection is performed by using an electrochemical or optical method, and the reading is immediately given. Typical biosensor-based commercial POCT devices are represented by blood glucose meters, blood gas analyzers, and are mainly used for blood glucose monitoring, blood gas and electrolyte analysis.
Aiming at a biochemical detection consumable device for simultaneously detecting a plurality of material indexes at one time, related technology exists at present, and specifically, microelectrodes for detecting specific biochemical molecules are integrated on an integral detection device and are applied to multi-parameter electrochemical detection. At present, the technology for combining a microfluidic system with POCT products exists at home and abroad. The microfluidic is a technology for controlling and operating a microfluidic on a chip with a micro-pipeline, and the conventional POCT related microfluidic system has defects such as solidification and blockage of liquid to be detected in the micro-fluidic pipeline, easy backflow and miscibility of the liquid to be detected, low liquid flow rate, low detection efficiency, influence of waste gas generated by reaction and the like.
In view of the above, the present invention aims to provide a single-electrode card micro-fluidic chip for multi-parameter biochemical molecular detection, which integrates the functions of preventing solidification and blockage of a liquid to be detected, mixing a calibration solution, detecting a sample solution, recovering waste liquid, discharging waste gas, etc. on one chip, reduces the cost and processing difficulty of the chip, ensures simultaneous detection of multiple parameters, improves the detection speed, sensitivity and accuracy, and realizes simultaneous detection of multiple indexes in biochemical electrolyte.
Disclosure of Invention
In summary, the invention provides a single-electrode card micro-fluidic chip for multi-parameter biochemical molecule detection.
The invention aims to provide a single-electrode card micro-fluidic chip for multi-parameter biochemical molecule detection, which specifically comprises a chip main body, a sample inlet, a sample pipeline system, a calibration liquid bag, an electrode card slot and a liquid guiding-out system, wherein the sample inlet, the sample pipeline system, the calibration liquid bag, the electrode card slot and the liquid guiding-out system are arranged on the chip main body; the sample injection pipeline system comprises a miniature check valve, the electrode clamping groove is connected with the sample injection pipeline system through a sample injection liquid channel buried in the chip main body, the sample injection liquid channel is communicated with the miniature check valve, and at least one calibration liquid channel extends out of the miniature check valve to be connected with the calibration liquid bag; one end of the electrode clamping groove is connected with the sample liquid channel, and the other end of the electrode clamping groove is communicated with the liquid guiding-out system through the sample liquid channel buried in the chip main body; the liquid guiding-out system is communicated with an exhaust hole.
Further, the width of the chip main body is 30-40 mm, and the height is 65-75 mm; the width of the sample inlet pipeline is 0.5-1.5 mm, the height is 0.5-1.5 mm, the width of the sample inlet liquid channel and the width of the calibration liquid channel are 0.5-1.5 mm, and the height is 0.5-1.5 mm; the width of the calibration liquid bag is 12.5-13.5 mm, and the height is 30.5-31.5 mm.
Further, the electrode clamping groove can be loaded with a single-chip electrode card, the contact surface of the electrode card and the chip main body is provided with an electrode detection site, the back surface of the electrode card is provided with a metal site, a sample test groove is arranged in the electrode clamping groove, the sample test groove is a part of a sample injection liquid channel and is connected with the sample discharge liquid channel, and the sample test groove is arranged at the position where the electrode detection site is located; the electrode clamping groove is internally provided with an electrode clamping positioning column, the surface of the electrode clamping is provided with a positioning hole, and the electrode clamping positioning column corresponds to the positioning hole in position and is consistent in size.
Further, the sample test slot is a fixed volume slot.
Further, the width of the sample test groove is 7.5-8.5 mm, and the height is 1.0-2.0 mm; the electrode clamp positioning column is cylindrical, and the radius of the bottom surface is 0.2mm; the width of the electrode clamping groove is 13.0-14.0 mm, and the height is 6.5-7.5 mm.
Further, the liquid delivery system includes a check valve, a waste tank, and an exhaust vent.
Further, the check valve structure includes a tesla valve structure.
Further, the chip main body is also provided with a chip positioning hole.
As shown in figure 1, the structure of the single-electrode card microfluidic chip for multi-parameter biochemical molecule detection is schematically shown.
The sample inlet can inject about 100 microliters of blood/test sample into the microfluidic pipeline through the needle tube of 1-3 milliliters; the miniature check valve has a miniature inverted truncated cone-shaped structure, when the chip is horizontally placed, the opening is large at the top and small at the bottom, and the backflow of a sample is prevented through the flowing resistance; the calibration liquid package is used for loading calibration liquid with standard concentration, and the calibration liquid can flow out through a mechanical device extrusion mode or a manual extrusion mode and the like.
The electrode clamping groove is used for detecting a sample by digging out a region on the chip main body, a piece of electrode card can be loaded for detecting the sample, a groove with a fixed volume is arranged in the electrode clamping groove and used as a sample test groove, so that the detection result is more accurate, and meanwhile, electrode card positioning columns are arranged at four corners or any two corners of the electrode clamping groove. Five microelectrodes are arranged on the front face of the electrode card, the positions of the microelectrodes are electrode card detection sites, and positioning holes are arranged at four corners or any two corners.
The positions of the sample test groove and the electrode card positioning column correspond to the microelectrode detection site and the positioning hole of the electrode card respectively, the electrode card can be installed and fixed in the electrode card groove, and meanwhile, the position of the microelectrode is positioned in the sample test groove; the sample liquid channel buried in the chip main body sequentially passes through the sample testing groove positions, is connected with the sample liquid channel at the outlet, and enters the waste liquid groove after passing through the check valve, wherein generated gas is discharged through the exhaust hole connected with the waste liquid groove. The check valve adopts a special structure to prevent the liquid in the waste liquid tank from flowing backwards; the waste liquid tank can be loaded with tested liquid, including calibration liquid and test sample, and the exhaust hole is used for exhausting gas in the flow channel, so that the liquid can flow in the micro flow channel conveniently, and the waste liquid tank has a simple anti-overflow structure.
The size of the electrode clamping groove and the size of the electrode card can be changed according to requirements, the number of the positioning columns can be changed according to the number and the size of the electrode card, and the number of microelectrodes of the electrode card can be adjusted according to detection requirements.
All the structures are contained in the chip main body and can be processed at one time by an injection molding method without additionally installing parts, so that the processing steps of the microfluidic system are greatly simplified, and the integration of the microfluidic and the sensing chip is realized.
Fig. 2 shows a schematic diagram of liquid sample injection of a single-electrode card microfluidic chip for multi-parameter biochemical molecule detection. When the sample is added, the microfluidic chip is horizontally placed, the front face is upward, the liquid to be measured is injected from the sample inlet and flows through the miniature check valve, meanwhile, a mechanical device or external force is used for extruding the calibration liquid bag, the calibration liquid in the calibration liquid bag is extruded and flows out, flows into the calibration liquid channel, and is mixed with the liquid to be measured through the miniature check valve; the mixed liquid sequentially passes through the sample liquid channel, the sample testing groove and the electrode detection site contacted with the electrode card, then passes through the sample liquid channel, flows through the simple check valve, enters the waste liquid groove, generates gas in the process and is discharged through the exhaust hole connected with the waste liquid groove, and the detection electric signal is transmitted through the metal site of the electrode card.
The beneficial effects of the invention are as follows:
(1) According to the invention, through the design of a microfluidic structure, the functions of preventing solidification and blockage of liquid to be detected, mixing of calibration liquid, detection of sample liquid, recovery of waste liquid, exhaust emission and the like are concentrated on one chip, so that the cost and processing difficulty of the chip are reduced, the simultaneous detection of multiple parameters is ensured, the detection speed, sensitivity and accuracy are improved, and the simultaneous detection of multiple indexes in biochemical electrolyte is realized.
(2) According to the microfluidic chip, the sample test groove is designed, the measurement volume is fixed, the measurement result is more accurate, meanwhile, the fixed electrode card positioning column is designed, so that the microfluidic chip and the electrode card can be separated, the structure of the microfluidic chip is simplified, the production process difficulty and the production efficiency of the microfluidic chip are greatly reduced, the electrode card can be installed according to a detection target, and the microfluidic chip is suitable for blood gas detection and multi-parameter electrochemical detection.
Drawings
The invention will be further described with reference to the accompanying drawings, in which embodiments do not constitute any limitation of the invention, and other drawings can be obtained by one of ordinary skill in the art without inventive effort from the following drawings.
FIG. 1 is a schematic structural diagram of a single-electrode card microfluidic chip for multi-parameter biochemical molecule detection;
FIG. 2 is a schematic diagram of liquid sample injection of a single-electrode card microfluidic chip for multi-parameter biochemical molecule detection.
The attached drawings are identified:
1. a sample inlet; 2. a miniature check valve; 3. a calibration liquid bag; 4. an electrode clamping groove; 5. a sample test slot; 6. an electrode card positioning column; 7. a non-return valve; 8. a waste liquid tank; 9. an exhaust hole; 10. and positioning holes.
Detailed Description
The invention will be further described in detail with reference to the following specific examples, with reference to the accompanying drawings, in order to make the objects, technical solutions and advantages of the invention more apparent.
Example 1
Referring to fig. 1, the single-electrode card micro-fluidic chip for multi-parameter biochemical molecule detection provided by the invention specifically comprises a chip main body, wherein the width of the chip main body is 35mm, the height of the chip main body is 70mm, and the thickness of the chip main body is 3.0mm.
In this embodiment, the sample inlet 1 on the chip main body is provided with a sample inlet pipe inside, and the width is 1.0mm, and the height is 1.0mm.
In the embodiment, a sample injection pipeline system and a calibration liquid bag 3 are arranged inside the chip main body, and the width of the calibration liquid bag 3 is 13mm and the height is 31mm.
In this embodiment, the sample injection pipeline system includes a micro check valve 2 and an electrode clamping groove 4, which are connected to the sample injection pipeline system through a sample injection liquid channel buried in the chip main body, the sample injection liquid channel is connected to the micro check valve 4, and a calibration liquid channel is extended from the micro check valve 2 to be connected to the calibration liquid bag 3.
The width of the sample injection liquid channel and the calibration liquid channel is 1.0mm, and the height is 1.0mm.
In this embodiment, a liquid guiding system connected to the sample liquid channel via the electrode slot 4 is further disposed inside the chip body.
In the embodiment, one end of the electrode clamping groove 4 is connected with the sample liquid channel, and the other end of the electrode clamping groove is communicated with the liquid guiding-out system through the sample liquid channel buried in the chip main body; the liquid delivery system is in communication with an exhaust vent 10.
In this embodiment, a piece of electrode card can be loaded in the electrode card slot 4, the contact surface of the electrode card and the chip main body is provided with an electrode detection site, namely a microelectrode, the back surface of the electrode card is provided with a metal site, the electrode card slot 4 is internally provided with a sample test slot 5, a sample liquid channel sequentially passes through the sample test slot 5 and contacts with the microelectrode of the electrode card, so as to realize a sample detection function, and is connected with a sample outlet liquid channel, and the sample test slot 5 is arranged at the position of the electrode detection site; the electrode clamping groove 4 is internally provided with an electrode clamping positioning column 6, the surface of the electrode clamping is provided with a positioning hole, and the electrode clamping positioning column 6 corresponds to the positioning hole in position and is consistent in size.
Five microelectrodes are integrated in the front detection area of the electrode card, wherein the microelectrode comprises a reference electrode, a counter electrode and three working electrodes, one working electrode can be used as a calibration electrode, and the electrode card can realize simultaneous detection of 2-3 indexes.
The sample test groove 5 is a groove with a fixed volume, the width of the groove is 8.1mm, and the height of the groove is 1.5mm; the positioning column is cylindrical, and the radius is 0.2mm; the electrode clamping groove 4 has a width of 13.4mm and a height of 7.0mm.
In this embodiment, the liquid delivery system includes a check valve 7, a waste liquid tank 8, and an exhaust hole 9.
The check valve 7 is of a tesla valve structure.
In this embodiment, the chip main body is further provided with a chip positioning hole 10, and the aperture is 1.0mm.
Example 2
The embodiment provides a single-electrode card micro-fluidic chip for multi-parameter biochemical molecule detection, which specifically comprises a chip main body, wherein the width of the chip main body is 35mm, the height of the chip main body is 70mm, and the thickness of the chip main body is 3.5mm.
In this embodiment, the sample inlet 1 on the chip main body is provided with a sample inlet pipe inside, the width is 1.5mm, and the height is 1.5mm.
In the embodiment, a sample injection pipeline system and a calibration liquid bag 3 are arranged inside the chip main body, and the width of the calibration liquid bag 3 is 12mm and the height is 30mm.
In this embodiment, the sample injection pipeline system includes a micro check valve 2 and an electrode clamping groove 4, which are connected to the sample injection pipeline system through a sample injection liquid channel buried in the chip main body, the sample injection liquid channel is connected to the micro check valve 4, and a calibration liquid channel is extended from the micro check valve 2 to be connected to the calibration liquid bag 3.
The width of the sample injection liquid channel and the calibration liquid channel is 1.0mm, and the height is 1.0mm.
In this embodiment, a liquid guiding system connected to the sample liquid channel via the electrode slot 4 is further disposed inside the chip body.
In the embodiment, one end of the electrode clamping groove 4 is connected with the sample liquid channel, and the other end of the electrode clamping groove is communicated with the liquid guiding-out system through the sample liquid channel buried in the chip main body; the liquid delivery system is in communication with an exhaust vent 10.
In this embodiment, a piece of electrode card can be loaded in the electrode card slot 4, the contact surface of the electrode card and the chip main body is provided with an electrode detection site, namely a microelectrode, the back surface of the electrode card is provided with a metal site, the electrode card slot 4 is internally provided with a sample test slot 5, a sample liquid channel sequentially passes through the sample test slot 5 and contacts with the microelectrode of the electrode card, so as to realize a sample detection function, and is connected with a sample outlet liquid channel, and the sample test slot 5 is arranged at the position of the electrode detection site; the electrode clamping groove 4 is internally provided with an electrode clamping positioning column 6, the surface of the electrode clamping is provided with a positioning hole, and the electrode clamping positioning column 6 corresponds to the positioning hole in position and is consistent in size.
Seven microelectrodes are integrated in the detection area on the front face of the electrode card, wherein the microelectrode comprises two reference electrodes, one counter electrode and four working electrodes, one working electrode can be used as a calibration electrode, and the electrode card can realize simultaneous detection of 3-4 indexes.
The sample test groove 5 is a groove with a fixed volume, the width of the groove is 8.0mm, and the height of the groove is 1.5mm; the positioning column is cylindrical, and the radius is 0.2mm; the electrode clamping groove 4 has a width of 14.5mm and a height of 7.5mm.
In this embodiment, the liquid delivery system includes a check valve 7, a waste liquid tank 8, and an exhaust hole 9.
The check valve 7 is of a tesla valve structure.
In this embodiment, the chip main body is further provided with a chip positioning hole 10, and the aperture is 1.0mm.
Example 3
The embodiment provides a single-electrode card micro-fluidic chip for multi-parameter biochemical molecule detection, which specifically comprises a chip main body, wherein the width of the chip main body is 35mm, the height of the chip main body is 70mm, and the thickness of the chip main body is 2.5mm.
In this embodiment, the sample inlet 1 on the chip main body is provided with a sample inlet pipe inside, the width is 1.2mm, and the height is 1.2mm.
In the embodiment, a sample injection pipeline system and a calibration liquid bag 3 are arranged inside the chip main body, and the width of the calibration liquid bag 3 is 12mm and the height is 30mm.
In this embodiment, the sample injection pipeline system includes a micro check valve 2 and an electrode clamping groove 4, which are connected to the sample injection pipeline system through a sample injection liquid channel buried in the chip main body, the sample injection liquid channel is connected to the micro check valve 4, and a calibration liquid channel is extended from the micro check valve 2 to be connected to the calibration liquid bag 3.
The width of the sample injection liquid channel and the calibration liquid channel is 1.2mm, and the height is 1.2mm.
In this embodiment, a liquid guiding system connected to the sample liquid channel via the electrode slot 4 is further disposed inside the chip body.
In the embodiment, one end of the electrode clamping groove 4 is connected with the sample liquid channel, and the other end of the electrode clamping groove is communicated with the liquid guiding-out system through the sample liquid channel buried in the chip main body; the liquid delivery system is in communication with an exhaust vent 10.
In this embodiment, a piece of electrode card can be loaded in the electrode card slot 4, the contact surface of the electrode card and the chip main body is provided with an electrode detection site, namely a microelectrode, the back surface of the electrode card is provided with a metal site, the electrode card slot 4 is internally provided with a sample test slot 5, a sample liquid channel sequentially passes through the sample test slot 5 and contacts with the microelectrode of the electrode card, so as to realize a sample detection function, and is connected with a sample outlet liquid channel, and the sample test slot 5 is arranged at the position of the electrode detection site; the electrode clamping groove 4 is internally provided with an electrode clamping positioning column 6, the surface of the electrode clamping is provided with a positioning hole, and the electrode clamping positioning column 6 corresponds to the positioning hole in position and is consistent in size.
Nineteen microelectrodes are integrated in the detection area on the front face of the electrode card, wherein the detection area comprises four reference electrodes, three counter electrodes and twelve working electrodes, wherein a module is formed by taking one auxiliary electrode as the center, the four working electrodes and the two reference electrodes, and the microelectrodes are integrated by integrating the three modules, so that the simultaneous detection of 12 indexes can be realized by the electrode card.
The sample test groove 5 is a groove with a fixed volume, the width of the groove is 8.5mm, and the height of the groove is 2.5mm; the positioning column is cylindrical, and the radius is 0.2mm; the electrode clamping groove 4 has a width of 14.0mm and a height of 7.0mm.
In this embodiment, the liquid delivery system includes a check valve 7, a waste liquid tank 8, and an exhaust hole 9.
The check valve 7 is of a tesla valve structure.
In this embodiment, the chip main body is further provided with a chip positioning hole 10, and the aperture is 1.0mm.
Example 4
The single-electrode card microfluidic chip for multi-parameter biochemical molecule detection provided in example 1 is shown in fig. 2, and is a schematic diagram of liquid sample injection of the single-electrode card microfluidic chip for multi-parameter biochemical molecule detection.
When in sample adding, the microfluidic chip is horizontally placed, the front face is upward, the liquid to be measured is injected from the sample inlet 1 and flows through the miniature check valve 2, meanwhile, the calibration liquid in the calibration liquid bag 3 is extruded and flows out by using a mechanical device or external force to extrude the calibration liquid bag 3, flows into a calibration liquid channel, and is mixed with the liquid to be measured through the miniature check valve 2; the mixed liquid sequentially passes through the sample liquid channel, the sample test groove 5 and the electrode detection site contacted with the electrode card, then passes through the sample liquid channel, flows through the simple check valve 7, enters the waste liquid groove 8, gas generated in the process is discharged through the exhaust hole 9 connected with the waste liquid groove 8, and the detection electric signal is transmitted through the metal site of the electrode card.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined appropriately to form other embodiments that will be understood by those skilled in the art. Technical details not described in detail in the present invention may be implemented by any prior art in the field. In particular, all technical features not described in detail in this invention can be realized by any prior art technique.
Claims (9)
1. The single-electrode card micro-fluidic chip for multi-parameter biochemical molecule detection is characterized by comprising a chip main body, a sample inlet (1), a sample pipeline system, a calibration liquid bag (3), an electrode clamping groove (4) and a liquid guiding-out system, wherein the sample inlet (1) is arranged on the chip main body; the sample injection pipeline system comprises a miniature check valve (2), the electrode clamping groove (4) is connected with the sample injection pipeline system through a sample injection liquid channel buried in the chip main body, the sample injection liquid channel is communicated with the miniature check valve (2), and at least one calibration liquid channel extends out of the miniature check valve (2) to be connected with the calibration liquid bag (3); one end of the electrode clamping groove (4) is connected with the sample injection liquid channel, and the other end of the electrode clamping groove is communicated with the liquid guiding-out system through a sample outlet liquid channel buried in the chip main body; the liquid guiding-out system is communicated with an exhaust hole (10).
2. The single-electrode card micro-fluidic chip for multi-parameter biochemical molecular detection according to claim 1, wherein the width of the chip main body is 30-40 mm, and the height is 65-75 mm; the width of the sample inlet (1) pipeline is 0.5-1.5 mm, the height of the sample inlet pipeline is 0.5-1.5 mm, the width of the sample liquid channel and the width of the calibration liquid channel are 0.5-1.5 mm, and the height of the sample liquid channel and the calibration liquid channel are 0.5-1.5 mm; the width of the calibration liquid bag (3) is 12.5-13.5 mm, and the height is 30.5-31.5 mm.
3. The single-electrode card micro-fluidic chip for multi-parameter biochemical molecular detection according to claim 1, wherein the electrode card slot (4) can be loaded with a single-sheet electrode card, the contact surface of the electrode card and the chip main body is provided with an electrode detection site, the back surface of the electrode card is provided with a metal site, a sample test slot (5) is arranged in the electrode card slot (4), the sample test slot (5) is a part of the sample introduction liquid channel and is connected with the sample discharge liquid channel, and the sample test slot (5) is arranged at the position of the electrode detection site; an electrode card positioning column (6) is arranged in the electrode card groove (4), a positioning hole is formed in the surface of the electrode card, and the electrode card positioning column (6) corresponds to the positioning hole in position and is consistent in size.
4. A single-electrode card microfluidic chip for multi-parameter biochemical molecular detection according to claim 3, wherein the sample test groove (5) is a groove of fixed volume.
5. The single-electrode card micro-fluidic chip for multi-parameter biochemical molecular detection according to claim 3, wherein the width of the sample test groove (5) is 7.5-8.5 mm, and the height is 1.0-2.0 mm; the electrode clamp positioning column (6) is cylindrical, and the radius of the bottom surface is 0.2mm; the width of the electrode clamping groove (4) is 13.0-14.0 mm, and the height is 6.5-7.5 mm.
6. The single-electrode card microfluidic chip for multi-parameter biochemical molecular detection according to claim 1, wherein the liquid guiding-out system comprises a check valve (7), a waste liquid tank (8) and an exhaust hole (9).
7. The single-electrode card microfluidic chip for multi-parameter biochemical molecular detection according to claim 6, wherein the check valve (7) structure comprises a tesla valve structure.
8. The single-electrode card microfluidic chip for multi-parameter biochemical molecular detection according to claim 1, wherein the chip main body is further provided with a chip positioning hole (10).
9. The application method of the single-electrode card micro-fluidic chip for multi-parameter biochemical molecule detection is characterized by comprising the following steps of: when in sample adding, the microfluidic chip is horizontally placed, the front face is upward, liquid to be measured is injected from the sample inlet (1) and flows through the miniature check valve (2) along the sample liquid channel, meanwhile, a mechanical device or manual extrusion calibration liquid bag (3) is used for extruding and flowing out the calibration liquid in the calibration liquid bag (3), flows into the calibration liquid channel, and is mixed with the liquid to be measured through the miniature check valve (2); the mixed liquid passes through the sample liquid channel, passes through the sample test groove (5) and the electrode detection site contacted with the electrode card, passes through the sample liquid channel, flows through the check valve (7) and enters the waste liquid groove (8), gas generated in the process is discharged through the exhaust hole (9) connected with the waste liquid groove (8), and the detection electric signal is transmitted through the metal site of the electrode card.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310428705.2A CN116351490A (en) | 2023-04-20 | 2023-04-20 | Single-electrode card micro-fluidic chip for multi-parameter biochemical molecule detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202310428705.2A CN116351490A (en) | 2023-04-20 | 2023-04-20 | Single-electrode card micro-fluidic chip for multi-parameter biochemical molecule detection |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116539701A (en) * | 2023-07-07 | 2023-08-04 | 北京几何科技有限公司 | Reagent card for urine detection and detection method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116539701A (en) * | 2023-07-07 | 2023-08-04 | 北京几何科技有限公司 | Reagent card for urine detection and detection method |
| CN116539701B (en) * | 2023-07-07 | 2023-09-15 | 北京几何科技有限公司 | Reagent card for urine detection and detection method |
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