CN116178571A - Endoplasmic reticulum targeting artificial protein, recombinant saccharomyces cerevisiae, endoplasmic reticulum targeting vesicle, immunoadjuvant and vaccine - Google Patents
Endoplasmic reticulum targeting artificial protein, recombinant saccharomyces cerevisiae, endoplasmic reticulum targeting vesicle, immunoadjuvant and vaccine Download PDFInfo
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- CN116178571A CN116178571A CN202310144827.9A CN202310144827A CN116178571A CN 116178571 A CN116178571 A CN 116178571A CN 202310144827 A CN202310144827 A CN 202310144827A CN 116178571 A CN116178571 A CN 116178571A
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- endoplasmic reticulum
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Abstract
The invention provides endoplasmic reticulum targeting artificial protein, recombinant saccharomyces cerevisiae, endoplasmic reticulum targeting vesicle, immunoadjuvant and vaccine, and belongs to the technical field of biomedicine and drug delivery. The endoplasmic reticulum targeting artificial protein provided by the invention targets the endoplasmic reticulum of eukaryotic cells through Pardaxin peptide, is anchored on vesicles through Atg8 fragments and self-assembles, so that the endoplasmic reticulum targeting vesicles with the Pardaxin targeting polypeptides displayed on the surfaces are formed. The invention also provides an endoplasmic reticulum targeting magnetic vesicle, which is obtained by coating the endoplasmic reticulum targeting vesicle prepared by the preparation method according to the scheme with the magnetic mesoporous silicon nanoparticle. The invention provides a novel broad-spectrum vaccine adjuvant for different pathogenic infections and provides a new idea for the preparation and production of synthetic biological vaccines.
Description
Technical Field
The invention belongs to the technical field of biomedicine and drug delivery, and particularly relates to an endoplasmic reticulum targeting artificial protein, recombinant saccharomyces cerevisiae, endoplasmic reticulum targeting vesicle, an immunoadjuvant and a vaccine.
Background
Endoplasmic reticulum (Endoplasmic reticulum, ER), one of the most important membranous organelles in eukaryotic cells, is the primary site of protein folding and transport, lipid synthesis, vesicle transport, calcium ion storage, and is involved in the regulation of multiple signal transduction pathways. Furthermore, the endoplasmic reticulum also establishes a connecting site with other membranous structures such as plasma membrane, mitochondria, endocytosis, lysosome and the like through a developed extension structure, and plays an important role in the information, substance and energy exchange process of intracellular organelles and plasma membranes. Given the extreme importance of the endoplasmic reticulum to the metabolic processes of eukaryotic cell growth, disorders in structure and function will have a significant impact on eukaryotic cells and organisms. Numerous studies have found that endoplasmic reticulum dysfunction is closely linked to numerous major human diseases found to date, such as cancer, autoimmune diseases, pathogenic microbial infections, neurodegenerative diseases, diabetes, etc. Along with the continuous development of accurate medical theory and technology, the development of drugs with accurate endoplasmic reticulum targeting becomes an important trend for preventing and overcoming related diseases.
The endoplasmic reticulum is inseparable from the synthesis, processing and transport of intracellular proteins. Based on the search for intracellular transport of proteins, more signal peptides with endoplasmic reticulum tropism were found. These endoplasmic reticulum signal peptides are modified at the N-terminus of the protein and perform different functions depending on their sequence. Endoplasmic reticulum signal peptides include KDEL peptides, eriss peptides, pardaxin peptides, and the like. Among them, pardaxin peptide (GFFALIPKIISSPLFKTLLSAVGSALSSSGGQE, SEQ ID No. 1) is a natural antimicrobial peptide with endoplasmic reticulum localization ability, and its endoplasmic reticulum targeting mechanism may be related to efficient phosphatidylcholine vesicle pore-forming ability (phosphatidylcholine is the main glycerophospholipid of endoplasmic reticulum, 60% of the ratio).
At present, there is no description about the preparation of endoplasmic reticulum targeting vesicles using Pardaxin peptides.
Disclosure of Invention
In view of the above, the present invention aims to provide an endoplasmic reticulum targeting artificial protein, recombinant saccharomyces cerevisiae, endoplasmic reticulum targeting vesicle, immunoadjuvant and vaccine, and the endoplasmic reticulum targeting artificial protein can be used for preparing the endoplasmic reticulum targeting vesicle.
The invention provides endoplasmic reticulum targeting artificial protein, which has an amino acid sequence shown as SEQ ID NO.2 from N end to C end.
The invention also provides a coding gene of the endoplasmic reticulum targeting artificial protein, and the nucleotide sequence of the coding gene is shown as SEQ ID NO. 3.
The invention also provides a recombinant saccharomyces cerevisiae, which takes ura 3-defective saccharomyces cerevisiae as an original cell and comprises recombinant plasmids; the recombinant plasmid is inserted with the coding gene of the scheme.
The invention also provides application of the artificial protein or the coding gene or the recombinant saccharomyces cerevisiae in preparation of endoplasmic reticulum targeting vesicles.
The invention also provides a preparation method of the endoplasmic reticulum targeting vesicle, which comprises the following steps:
inoculating the recombinant saccharomyces cerevisiae in the scheme to an SC-Gal culture medium, and performing first induction culture;
inoculating the recombinant saccharomyces cerevisiae subjected to the first induction culture to an SC-Gal culture medium without a nitrogen source, and performing a second induction culture;
centrifuging the culture after the second induction culture, collecting precipitated yeast cells, and extracting protoplasts of the yeast cells to obtain yeast protoplasts;
crushing and ultracentrifugation are sequentially carried out on the yeast protoplast, and sediment is collected to obtain a crude product of the endoplasmic reticulum targeting vesicle;
purifying the crude vesicle product by adopting a nickel column to obtain an endoplasmic reticulum targeting vesicle;
the SC-Gal culture medium takes water as a solvent and comprises the following components in concentration: 6.7g/L of nitrogen source of the yeast without amino group, 20g/L of galactose and 2g/L of mixed amino acid;
the SC-Gal culture medium without the nitrogen source of the amino-free yeast takes water as a solvent and comprises the following components in concentration: galactose 20g/L and mixed amino acid 2g/L;
the mixed amino acid comprises the following components in parts by mass: 2 parts of L-proline, 10 parts of L-leucine, 2 parts of L-valine, 2 parts of L-alanine, 2 parts of L-serine, 2 parts of L-lysine, 2 parts of L-glutamic acid, 2 parts of L-methionine, 2 parts of L-threonine, 2 parts of L-glutamine, 2 parts of L-glycine, 2 parts of L-isoleucine, 2 parts of L-tryptophan, 2 parts of L-aspartic acid, 2 parts of L-tyrosine, 2 parts of L-phenylalanine, 2 parts of inositol, 2 parts of L-cysteine, 2 parts of p-aminobenzoic acid, 2 parts of L-asparagine and 0.5 part of adenine.
The invention also provides an endoplasmic reticulum targeting magnetic vesicle, which comprises magnetic mesoporous silicon nano particles and endoplasmic reticulum targeting vesicles coated in pore channels of the magnetic mesoporous silicon nano particles, wherein the endoplasmic reticulum targeting vesicles are prepared by the preparation method according to the scheme.
Preferably, the inner core of the magnetic mesoporous silicon nanoparticle is MnFe 2 O 4 A nanoparticle; the shell of the magnetic mesoporous silicon nanoparticle is mesoporous silicon dioxide; the preparation method of the magnetic mesoporous silicon nanoparticle comprises the following steps:
MnFe is mixed with 2 O 4 Suspending in an organic solvent to obtain a suspension;
firstly mixing the suspension, the pore-forming agent and water, and removing the organic solvent to obtain a first mixed solution;
mixing the first mixed solution, water, methanol and ethyl acetate for the second time to obtain a second mixed solution;
thirdly mixing the second mixed solution, an ammonium hydroxide solution, tetraethoxysilane and 3-aminopropyl triethoxysilane to obtain a third mixed solution;
and centrifuging the third mixed solution, collecting precipitate, washing the precipitate, and removing the pore-forming agent to obtain the magnetic mesoporous silicon nanoparticle.
The invention also provides application of the endoplasmic reticulum targeting magnetic vesicle in preparation of an immune adjuvant.
The invention also provides a vaccine, which comprises magnetic mesoporous silicon nano particles, and endoplasmic reticulum targeting vesicles and antigens coated in pore channels of the magnetic mesoporous silicon nano particles, wherein the endoplasmic reticulum targeting vesicles are prepared by the preparation method according to the scheme.
Preferably, the antigen comprises a pathogenic antigen or a tumor antigen.
The invention provides endoplasmic reticulum targeting artificial protein, which has an amino acid sequence shown as SEQ ID NO.2 from N end to C end. The endoplasmic reticulum targeting artificial protein contains an N-terminal 6 XHis tag, pardaxin peptide, green fluorescent protein and Saccharomyces cerevisiae Atg8 fragment. The endoplasmic reticulum targeting artificial protein provided by the invention targets the endoplasmic reticulum of eukaryotic cells through Pardaxin peptide, is anchored on vesicles through Atg8 fragments and self-assembles, so that the endoplasmic reticulum targeting vesicles with the Pardaxin targeting polypeptides displayed on the surfaces are formed.
The invention also provides an endoplasmic reticulum targeting magnetic vesicle, which comprises magnetic mesoporous silicon nano particles and endoplasmic reticulum targeting vesicles coated in pore channels of the magnetic mesoporous silicon nano particles, wherein the endoplasmic reticulum targeting vesicles are prepared by the preparation method according to the scheme.
The endoplasmic reticulum targeting magnetic vesicle is a broad-spectrum endoplasmic reticulum targeting magnetic vesicle adjuvant and can load antigens. The invention provides a novel broad-spectrum vaccine adjuvant for different pathogenic infections and provides a new idea for the preparation and production of synthetic biological vaccines.
Drawings
FIG. 1 shows MnFe 2 O 4 Characterization of the nanoparticles; wherein A is a transmission electron microscope image, and B is a magnetocaloric curve;
FIG. 2 is a representation of MagMSN and MagParV, where A is energy spectrum analysis, B is transmission electron microscopy, C is Zeta potential, D is antigen loading capacity, and E is antigen release capacity;
FIG. 3 is serum CaAg IgG levels in mice after immunization with free antigen or antigen-loaded adjuvant;
FIG. 4 is a mouse survival curve;
FIG. 5 is serum SaAg IgG levels in mice after immunization with free antigen or antigen-loaded adjuvant;
figure 6 shows the inhibition of tumor growth in mice by free antigen and antigen-loaded adjuvant immunization.
Detailed Description
The invention provides an endoplasmic reticulum targeting artificial protein ParGA protein, which has an amino acid sequence shown in SEQ ID NO.2 from N end to C end, and specifically comprises the following components:
MHHHHHHGFFALIPKIISSPLFKTLLSAVGSALSSSGGQEGGGGGGVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGGMKSTFKSEYPFEKRKAESERIADRFKNRIPVICEKAEKSDIPEIDKRKYLVPADLTVGQFVYVIRKRIMLPPEKAIFIFVNDTLPPTAALMSAIYQEHKDKDGFLYVTYSGENTFGR。
the endoplasmic reticulum targeting artificial protein contains an N-terminal 6 XHis tag, pardaxin peptide, green fluorescent protein and Saccharomyces cerevisiae Atg8 fragment. The artificial protein of the invention targets the endoplasmic reticulum of eukaryotic cells through Pardaxin peptide, anchors on vesicles through Atg8 fragments and self-assembles to form endoplasmic reticulum targeting vesicles with surface displaying Pardaxin targeting polypeptide. The 6 XHis tag and the green fluorescent protein respectively play roles of separating and purifying the tag and identifying.
The invention also provides a coding gene PARGA of the endoplasmic reticulum targeting artificial protein, which has the nucleotide sequence shown in SEQ ID NO.3, and specifically comprises the following steps:
atgcatcatc accaccatca cggtttcttt gctttgatcc caaagattat ctcttctccattgtttaagacattgttgtc agctgtcggt tccgctttgt cctcttccgg tggtcaagaaggtggcgggg gtggtggtgtgtccaaaggt gaagaattat tcactggtgt tgttccaatcttggttgaat tggatggtga tgttaacggtcacaagttct ccgtttctgg tgaaggtgaaggtgatgcta cctacggtaa gttgaccttg aaattcatctgtaccaccgg taagctgcccgtcccttggc caaccttggt caccactttg acctacggtg ttcaatgtttctctagatacccagaccaca tgaagcaaca cgacttcttc aagtccgcta tgccagaaggttacgtccaagaaagaacca ttttcttcaa ggatgacgga aactataaga ccagagctgaagtcaagtttgaaggtgaca ctttggtcaa ccgtatcgaa ttgaagggta ttgatttcaaggaagacggtaacatcttag gtcacaaatt ggaatacaac tacaactctc acaacgtctacattatggctgacaagcaaa agaacggtat caaggtcaat ttcaagatca gacacaacattgaggatggttctgtccaat tggctgatca ttaccaacaa aacactccaa ttggtgacggtccagtcttactacctgaca accactactt atccactcaa tctgctctct ccaaggacccaaacgaaaagagagatcaca tggttttgtt ggaatttgtt actgctgctg gtatcactttgggtatggacgaattgtaca agggtggtgg tatgaaatct actttcaaga gtgaatatccattcgaaaagagaaaggccg aatctgaacg tattgctgac agattcaaga acagaatcccagttatctgtgaaaaggccg aaaagtctga cattccagaa attgacaaaa gaaagtacttagttccagctgatttgactg ttggtcaatt cgtttacgtt attcgtaaga gaatcatgttgccaccagaaaaagctatat tcatttttgt taatgacacc ctacctccaa ctgccgccttgatgtctgccatctaccaag aacacaagga caaggatggt ttcttgtacg ttacttactctggtgaaaacaccttcggta gataa。
the coding gene is obtained based on optimization of the optimal codon of saccharomyces cerevisiae.
The invention also provides a recombinant saccharomyces cerevisiae, which takes ura 3-defective saccharomyces cerevisiae as an original cell and comprises recombinant plasmids; the recombinant plasmid is inserted with the coding gene of the scheme.
In the invention, the original plasmid of the recombinant plasmid is an expression plasmid of saccharomyces cerevisiae, preferably a plasmid pESC-URA of an inducible promoter PGAL1, and when the pESC-URA is taken as the original plasmid, the insertion site of the coding gene is BamH1/Xho1.
In the present invention, the ura 3-deficient Saccharomyces cerevisiae is preferably Saccharomyces cerevisiae Sc0, a chassis cell, genotype: MATahis 3.DELTA.1leu2 trp1-289 ura3-52.
The method for constructing the recombinant plasmid and the recombinant saccharomyces cerevisiae is not particularly limited, and conventional methods in the field can be adopted.
The invention also provides application of the artificial protein or the coding gene or the recombinant saccharomyces cerevisiae in preparation of endoplasmic reticulum targeting vesicles.
The invention also provides a preparation method of the endoplasmic reticulum targeting vesicle, which comprises the following steps:
inoculating the recombinant saccharomyces cerevisiae in the scheme to an SC-Gal culture medium, and performing first induction culture;
inoculating the recombinant saccharomyces cerevisiae subjected to the first induction culture to an SC-Gal culture medium without an amino-free yeast nitrogen source, and performing a second induction culture;
centrifuging the culture after the second induction culture, collecting precipitated yeast cells, and extracting protoplasts of the yeast cells to obtain yeast protoplasts;
crushing and ultracentrifugation are sequentially carried out on the yeast protoplast, and sediment is collected to obtain a crude product of the endoplasmic reticulum targeting vesicle;
purifying the crude vesicle product by adopting a nickel column to obtain an endoplasmic reticulum targeting vesicle;
the recombinant saccharomyces cerevisiae disclosed by the scheme is inoculated to an SC-Gal culture medium for first induction culture.
Before inoculating the recombinant saccharomyces cerevisiae into the SC-Gal medium, preferably further comprising inoculating a strain of the recombinant saccharomyces cerevisiae into the YPD liquid medium for expansion culture to obtain a large amount of recombinant saccharomyces cerevisiae; the temperature of the expansion culture is preferably 30 ℃; the mode of the expansion culture is preferably shake culture; the time for the expansion culture is preferably 9 to 16 hours, more preferably 12 hours.
In the invention, the SC-Gal culture medium takes water as a solvent and comprises the following components in concentration: 6.7g/L of nitrogen source of the yeast without amino group, 20g/L of galactose and 2g/L of mixed amino acid; the water is preferably distilled water; the pH value of the SC-Gal culture medium is preferably 6.0-6.5.
In the present invention, the amino-free yeast nitrogen source is preferably yeast nitrogen base (without amino acids), available from Solarbio under the specification of 500 g and model Y8040.
In the present invention, the temperature of the first induction culture is preferably 30 ℃; the first induction culture mode is preferably shake culture; the time of the first induction culture is preferably 24 hours; the first induction culture functions to induce the expression of ParGA under the control of GAL10 promoter.
After the first induction culture, the recombinant saccharomyces cerevisiae after the first induction culture is inoculated into an SC-Gal culture medium without a nitrogen source for the second induction culture.
In the invention, the SC-Gal culture medium which does not contain an amino-free yeast nitrogen source takes water as a solvent and comprises the following components in concentration: galactose 20g/L and mixed amino acid 2g/L; the water is preferably distilled water; the pH value of the SC-Gal culture medium without the nitrogen source of the amino-free yeast is preferably 6.0-6.5;
in the invention, the mixed amino acid comprises the following components in parts by mass: 2 parts of L-proline, 10 parts of L-leucine, 2 parts of L-valine, 2 parts of L-alanine, 2 parts of L-serine, 2 parts of L-lysine, 2 parts of L-glutamic acid, 2 parts of L-methionine, 2 parts of L-threonine, 2 parts of L-glutamine, 2 parts of L-glycine, 2 parts of L-isoleucine, 2 parts of L-tryptophan, 2 parts of L-aspartic acid, 2 parts of L-tyrosine, 2 parts of L-phenylalanine, 2 parts of inositol, 2 parts of L-cysteine, 2 parts of p-aminobenzoic acid, 2 parts of L-asparagine and 0.5 part of adenine.
In the present invention, the temperature of the second induction culture is preferably 30 ℃; the second induction culture mode is preferably shake culture, and the rotation speed of the shake culture is preferably 100-140 rpm; the time of the second induction culture is preferably 2 hours; the second induction culture is used for inducing the ParGA protein containing the Atg8 fragment to form autophagosome vesicles through the autophagy pathway by rapid self-assembly.
After the second induction culture is carried out, the culture after the second induction culture is centrifuged, precipitated yeast cells are collected, protoplasts of the yeast cells are extracted, and the yeast protoplasts are obtained. In the present invention, the rotational speed of the centrifugation is preferably 4200rpm, and the time of the centrifugation is preferably 3min. The invention preferably further comprises washing the collected yeast cells prior to extracting the protoplasts; the reagent used for the washing is preferably protoplast preparationA buffer; the protoplast preparation buffer uses water as a solvent, and preferably comprises the following components in concentration: sorbitol 1M, trisodium citrate 0.02. 0.02M, EDTANa 2 0.1 M and Na 2 HPO 4 ·12H 2 O 0.02M。
In the invention, the extracting protoplast preferably comprises mixing yeast cells after the second induction culture with enzyme for enzymolysis to obtain protoplast suspension, centrifuging the protoplast suspension, and collecting precipitated yeast protoplast; the enzyme preferably comprises yeast muramidase or helicase; the working concentration of the yeast muramidase is preferably 100U/mL; the working mass concentration of the snailase is preferably 2% (W/V); the enzymolysis time is preferably 10-30 min, more preferably 20min; the rotational speed of the centrifugation is preferably 4200rpm; the time of the centrifugation is preferably 5min.
After the yeast protoplast is obtained, the yeast protoplast is crushed and ultracentrifuged in sequence, and sediment is collected to obtain a crude product of the endoplasmic reticulum targeting vesicle.
In the present invention, the crushing mode is preferably homogenizing and crushing by using a Dounce homogenizer. In the present invention, the rotational speed of the ultracentrifugation is preferably 35000rpm; the time of the ultracentrifugation is preferably 30 to 120min, more preferably 60 to 90min.
After obtaining the crude product of the endoplasmic reticulum targeting vesicle, the invention adopts a nickel column to purify the crude product of the vesicle to obtain the endoplasmic reticulum targeting vesicle.
In the present invention, the purification of the crude vesicle product using a nickel column preferably comprises: suspending the crude endoplasmic reticulum targeting vesicle in 10mM imidazole buffer, adding into a nickel column for vesicle adsorption, washing with 10mM imidazole buffer, and eluting with 100mM imidazole; the number of times of washing is preferably 3.
The invention also provides an endoplasmic reticulum targeting magnetic vesicle, which comprises magnetic mesoporous silicon nano particles and endoplasmic reticulum targeting vesicles coated in pore channels of the magnetic mesoporous silicon nano particles, wherein the endoplasmic reticulum targeting vesicles are prepared by the preparation method according to the scheme.
In the invention, the mass ratio of the endoplasmic reticulum targeting vesicle to the magnetic mesoporous silicon nanoparticle is preferably (0.1-1): (1 to 10), more preferably 0.5:2.
in the invention, the endoplasmic reticulum targeting magnetic vesicle is circular; the particle size of the endoplasmic reticulum targeting magnetic vesicle is 100-200 nm; the aperture of the endoplasmic reticulum targeting magnetic vesicle is 10-20 nm, and each MagMSN center is provided with a plurality of MnFe 2 O 4 A cluster of nanoparticles; the Zeta potential of the MagMSN was positive (+26 mV).
In the invention, the inner core of the magnetic mesoporous silicon nanoparticle is MnFe 2 O 4 A nanoparticle; the shell of the magnetic mesoporous silicon nanoparticle is mesoporous silicon dioxide; the magnetic mesoporous silicon nanoparticle is preferably prepared by the following method:
MnFe is mixed with 2 O 4 Suspending in an organic solvent to obtain a suspension;
firstly mixing the suspension, the pore-forming agent and water, and removing the organic solvent to obtain a first mixed solution;
mixing the first mixed solution, water, methanol and ethyl acetate for the second time to obtain a second mixed solution;
thirdly mixing the second mixed solution, an ammonium hydroxide solution, tetraethoxysilane and 3-aminopropyl triethoxysilane to obtain a third mixed solution;
and centrifuging the third mixed solution, collecting precipitate, washing the precipitate, and removing the pore-forming agent to obtain the magnetic mesoporous silicon nanoparticle.
The invention firstly prepares ferromanganese (MnFe 2 O 4 ) Suspending in organic solvent to obtain suspension.
In the present invention, the MnFe 2 O 4 Preferably, trivalent organic iron and divalent organic manganese are respectively used as an iron source and a manganese source to carry out thermal decomposition reaction to obtain the iron-based alloy; the MnFe 2 O 4 Is a magnetic nanoparticle, the MnFe 2 O 4 The diameter of the nano particles is 9-11 nm, and the MnFe 2 O 4 The nano particles have good magnetocaloric conversion capability.
In the present invention, the iron (III) acetylacetonate is preferably iron (III) acetylacetonate; the divalent organic manganese is preferably manganese (II) acetylacetonate; the thermal decomposition reaction comprises a first thermal decomposition reaction and a second thermal decomposition reaction which are sequentially carried out; the temperature of the first thermal decomposition reaction is preferably 200 ℃; the time of the first thermal decomposition is preferably 1h; the temperature of the second thermal decomposition reaction is preferably 298 ℃; the time of the second thermal decomposition reaction is preferably 1h.
In the present invention, the organic solvent is preferably chloroform, and the MnFe 2 O 4 The ratio of the mass of (c) to the volume of the organic solvent is preferably 5mg:2mL.
After the suspension is obtained, the suspension, the pore-forming agent and water are mixed for the first time, and the organic solvent is removed to obtain a first mixed solution. In the present invention, the pore-forming agent is preferably cetyl trimethylammonium bromide (CTAB); the water is preferably distilled water; by MnFe 2 O 4 The amount of the pore-forming agent is preferably 200mg, and the amount of the water is preferably 10mL, based on 5 mg; the first mixing mode is preferably ultrasonic mixing; the time of the first mixing is preferably 20min; the organic solvent is preferably removed by evaporation of the organic solvent by heating to 75 ℃.
After the first mixed solution is obtained, the first mixed solution, water, methanol and ethyl acetate are mixed for the second time to obtain a second mixed solution. In the present invention, the water is preferably distilled water; the volume of the first mixed solution, water, methanol and ethyl acetate is preferably 10:95:5:20, a step of; the second mixing mode is preferably magnetic stirring mixing; the time of the second mixing is preferably 10 minutes.
After the second mixed solution is obtained, the second mixed solution, ammonium hydroxide solution, tetraethoxysilane (TEOS) and 3-aminopropyl triethoxysilane (APTES) are mixed in a third mode to obtain a third mixed solution. In the invention, the ammonium hydroxide solution is ammonia water, and the dosage of the ammonium hydroxide solution is preferably 3mL according to the volume of the first mixed solution being 10mL; the TEOS is preferably used in an amount of 300. Mu.L; the dosage of APTES is preferably 30 mu L; the third mixing mode is preferably magnetic stirring mixing, and mesoporous silicon grows on the surfaces of the magnetic nano particles in the magnetic stirring mixing process; the time of the third mixing is preferably 12 hours.
After the third mixed solution is obtained, the invention carries out centrifugation on the third mixed solution, collects sediment, washes the sediment and removes pore-forming agent to obtain the magnetic mesoporous silicon nano particles. In the invention, the rotation speed of the centrifugation is preferably 8000-10000 rpm, and the centrifugation time is preferably 10min; the reagent used for the washing is preferably ethanol; the removal of the pore-forming agent preferably comprises heating with an ammonium nitrate solution having a concentration of 200mg/100mL to remove the pore-forming agent from the pores; the solvent of the ammonium nitrate solution is preferably ethanol.
In the present invention, mnFe 2 O 4 Is an initial magnetic nanoparticle, after ammonium hydroxide solution, tetraethoxysilane and 3-aminopropyl triethoxysilane are added, the surface oxygen atom of the nanoparticle reacts with tetraethoxysilane, mesoporous silica is derived on the basis, and finally the magnetic mesoporous silicon nanoparticle with a core-shell structure is obtained, and the inner core of the magnetic mesoporous silicon nanoparticle is MnFe 2 O 4 The shell of the nanoparticle is mesoporous silica.
In the invention, the magnetic mesoporous silicon nanoparticle is MnFe wrapped by MSN with large aperture 2 O 4 Is a magnetic mesoporous silicon nanoparticle MagMSN.
The invention also provides application of the endoplasmic reticulum targeted magnetic vesicle in preparation of an immunoadjuvant and/or an anti-tumor drug.
The immune adjuvant is a broad-spectrum endoplasmic reticulum targeting magnetic vesicle adjuvant and can load antigen. The immunoadjuvant of the invention has higher antigen loading capacity, obviously induces the generation of antigen specific antibodies of fungi, bacteria and viruses under the treatment of alternating magnetic field, and can effectively protect organisms from serious pathogenic bacteria systemic infection. The invention provides a novel broad-spectrum vaccine adjuvant for different pathogenic infections and provides a new idea for the preparation and production of synthetic biological vaccines.
In the present invention, the antitumor drug achieves an antitumor effect by inhibiting tumor growth.
The invention also provides a vaccine, which comprises magnetic mesoporous silicon nano particles, and endoplasmic reticulum targeting vesicles and antigens coated in pore channels of the magnetic mesoporous silicon nano particles, wherein the endoplasmic reticulum targeting vesicles are prepared by the preparation method according to the scheme.
In the invention, both the endoplasmic reticulum targeting vesicle and the antigen are adsorbed into the pores of the magnetic mesoporous silicon nanoparticle.
In the present invention, the preparation method of the magnetic mesoporous silicon nanoparticle is the same as the above scheme, and will not be described here again.
In the present invention, the antigen preferably includes a pathogenic bacteria antigen or a tumor antigen; the pathogen antigen preferably comprises a pathogen protein antigen and or an inactivated virus antigen, more preferably comprises a pathogen protein; the pathogen proteins preferably include candida albicans antigen (CaAg), staphylococcus aureus antigen (SaAg); one or more of the avian influenza virus antigen (AIVAg) and SARS-CoV-2s protein receptor binding domain (SRBD); the tumor antigen preferably comprises a brain glioma antigen; the glioma antigen preferably comprises a glioma GL261 antigen.
In the present invention, the mass part of the endoplasmic reticulum targeting vesicle is preferably (0.1 to 1 part, more preferably 0.5 part) based on 1 part by mass of the antigen; the mass part of the magnetic mesoporous silicon nanoparticle is preferably 1-10 parts, more preferably 2 parts.
The invention also provides a preparation method of the vaccine according to the scheme, which comprises the following steps:
and mixing the magnetic mesoporous silicon nanoparticle, the antigen and the endoplasmic reticulum targeting vesicle to obtain the vaccine.
In the present invention, the mixing preferably mixes the antigen and the endoplasmic reticulum-targeted magnetic vesicle in PBS.
The invention also provides a using method of the vaccine, which comprises the following steps:
the vaccine is administered to the subject.
In the present invention, the amount of vaccination is preferably 20 to 100. Mu.g, more preferably 35 to 50. Mu.g, per mouse based on the total mass of antigen and endoplasmic reticulum targeting magnetic vesicles.
After inoculation, it is preferable to further include treating the vaccinated subject in an alternating magnetic field for 8 to 12 minutes, preferably 10 minutes. In the present invention, the frequency of the alternating magnetic field is preferably 50 to 500000Hz, more preferably 400 to 10000Hz; the power of the alternating magnetic field is preferably 10 to 5000W, more preferably 100 to 1000W.
In the present invention, since the vaccine contains superparamagnetic MnFe 2 O 4 The nano particles can generate heat under the alternating magnetic field treatment condition, so that the heat promotes the release of antigens, activates the immunogenicity of the antigens, obviously induces the generation of specific antibodies of fungi, bacteria, viruses and tumor antigens, effectively prevents pathogenic bacteria infection and inhibits tumor growth.
The technical solutions of the present invention will be clearly and completely described in the following in connection with the embodiments of the present invention.
EXAMPLE 1 preparation of recombinant Saccharomyces cerevisiae cells
Endoplasmic reticulum targeting artificial protein ParGA (the amino acid sequence is shown as SEQ ID NO. 2) is designed, and the protein contains an N-terminal 6 XHis tag, a pardaxin, green fluorescent protein and a Saccharomyces cerevisiae Atg8 fragment. Based on the optimal codon of Saccharomyces cerevisiae, an artificial gene PARGA (nucleotide sequence shown as SEQ ID NO. 3) for encoding the ParGA protein is designed. The artificial gene ParGA is cloned on a plasmid pESC-URA consisting of an inducible promoter PGAL1 to obtain the plasmid pESC-ParGA, and the plasmid pESC-ParGA is transformed into a chassis cell Saccharomyces cerevisiae Sc0 (MATA his3 delta 1leu2 trp1-289 URA 3-52) to obtain a recombinant Saccharomyces cerevisiae cell Sc0+ParV.
Example 2 preparation of biosynthetic vesicles
Reagent:
SC-Gal medium formulation: 0.67g of non-amino yeast nitrogen source, 2g of galactose, 0.2g of mixed amino acid powder and 100mL of sterile water;
the culture formula of the SC-Gal culture medium without nitrogen source comprises the following steps: galactose 2g, mixed amino acid powder 0.2g and sterile water 100mL;
protoplasm systemThe preparation buffer solution formula comprises the following steps: with water as solvent, sorbitol 1M, trisodium citrate 0.02. 0.02M, EDTANa 2 0.1 M and Na 2 HPO 4 ·12H 2 O 0.02M。
The method comprises the following steps:
the synthetic yeast cells Sc0+ParV were shake-cultured in YPD medium at 30℃overnight, transferred to SC-Gal medium, and shake-cultured at 30℃for 24 hours. And transferring to SC-Gal culture medium without nitrogen source, and shake culturing for 2 hr to form autophagosome vesicle. ParV-containing cells were collected, washed with protoplast preparation buffer, and then treated with yeast muramidase (100U/mL, 10 min) to obtain yeast protoplasts.
Breaking up yeast protoplasts with a Dounce homogenizer, ultracentrifugating at 35000rpm for 30min to separate ParV vesicles, finally suspending ParV vesicles in 10mM imidazole buffer, adding into a nickel column for vesicle adsorption, and then washing with 10mM imidazole buffer for 3 times; finally, 100mM imidazole was used to obtain purified ParV vesicles.
Example 3 preparation of biosynthetic vesicles
Example 2 was repeated except that the yeast muramidase (100U/mL, 10 min) treatment was replaced with the snailase (2%, 10 min) treatment.
Example 4 preparation of endoplasmic reticulum targeting magnetic vesicle-based anti-candida albicans vaccine
1. Preparation process
(1) Preparation of magnetic mesoporous silicon nano particles
Iron (III) acetylacetonate and manganese (II) acetylacetonate are used as iron sources and manganese sources, and a thermal decomposition method (200 ℃ C., 1h, then 298 ℃ C., 1 h) is adopted to synthesize MnFe 2 O 4 Magnetic nanoparticles.
5mg of MnFe 2 O 4 The nanoparticles were suspended in 2mL of chloroform, added to 10mL of distilled water containing 200mg CTAB, sonicated for 20min, and heated to 75℃to evaporate the chloroform. This solution was added to a mixture of 95mL distilled water, 5mL methanol and 20mL ethyl acetate, magnetically stirred for 10min, followed by addition of 3mL ammonium hydroxide, 300 μLTEOS and 30 μLAPTES. And magnetically stirring for 12h, centrifuging, and washing the precipitate with ethanol. 100m with 200mg of ammonium nitrateHeating the L-ethanol to remove CTAB in the pores. Finally obtaining the MnFe wrapped by the large-aperture MSN 2 O 4 Is a magnetic mesoporous silicon nanoparticle MagMSN.
(2) Preparation and characterization of anti-candida albicans vaccine
Centrifuging Candida albicans cells cultured overnight at 37deg.C, and re-suspending in sterile water to give a bacterial concentration of 5×10 8 cells/mL. Ultrasonic crushing, wherein ultrasonic conditions are as follows: the sample was ice-bathed, 100W, sonicated for 3s, stopped for 3s, and sonicated 100 times. Centrifuging at 12000rpm for 5min, and collecting supernatant to obtain Candida albicans antigen CaAg.
CaAg, magMSN and ParV vesicles prepared in example 2 were mixed in PBS at a mass ratio of 1:2: mixing thoroughly at a ratio of 0.5 to obtain the anti-candida albicans vaccine CaAg+MagParV. Simultaneously, the mass ratio is set to be 1:1: 1. 1:2:1 ratio control.
The candida albicans vaccine is characterized by adopting transmission electron microscope observation, zeta potential analysis, antigen release analysis and other methods.
(3) Performance evaluation of anti-candida albicans vaccine
Balb/c female mice were vaccinated with 35 μg CaAg+MagParV on day-14 (14 days before the date of vaccination with Candida albicans) and day-7 (7 days before the date of vaccination with Candida albicans), and treated with alternating magnetic fields (100-400 Hz, 100W) for 10min to complete immunization. Culturing Candida albicans SC5314 cells in YPD medium with 30 ° shaking overnight, and adjusting bacterial solution concentration to 5×10 in physiological saline 7 cells/mL. The candida albicans suspension was injected intravenously into non-immunized and immunized mice (100 μl/mouse) on day 0 and survival was recorded initially. Mouse serum was collected on day 0, day 2, day 4, day 8 and day 20, and infected mice were assayed for anti-CaAg IgG levels by ELISA.
2. Experimental results
(1) Characterization of broad-spectrum endoplasmic reticulum targeting magnetic vesicle adjuvants
The transmission electron microscope observation result shows that MnFe 2 O 4 The diameter of the nano particle is about 9-11 nm, and the magnetic core has good magnetocaloric conversion capability, and the solution temperature can reach 25-48 ℃ after the treatment of an alternating magnetic field for 10minFig. 1).
Transmission electron microscope observation and energy spectrum analysis show that the MagMSN is round, the grain diameter is 100-200 nm, the aperture is 10-20 nm, and each MagMSN center is provided with a plurality of MnFe 2 O 4 A cluster of nanoparticles; magParV is round, with a distinct coating on the surface, slightly increased in particle size compared to MagMSN, between 110 and 220nm (FIG. 2).
The Zeta potential analysis results showed that the Zeta potential of MagMSN was positive (+26 mV) and the potential of MagParV was negative (-10 mV) (fig. 2).
The mass ratio of the papain to CaAg, magMSN and ParV vesicles is 1:2: vaccine group 0.5, 1:1: 1. 1:2: in the control group with the ratio of 1, the nanoparticles have obvious antigen or vesicle distribution, which indicates that the antigen or vesicle in the two control groups is excessive, thereby determining that the optimal mass ratio of the three components is 1:2:0.5.
to investigate the antigen loading capacity of the broad spectrum endoplasmic reticulum-targeted magnetic vesicle adjuvants, magMSN and MagParV were incubated in different antigen solutions for 24h, including Ovalbumin (OVA), bovine Serum Albumin (BSA), candida albicans antigen (CaAg), staphylococcus aureus antigen (SaAg), avian influenza virus antigen (AIVAg) and SARS-CoV-2s protein receptor binding domain (SRBD), and as a result, magParV was shown to be loaded with higher levels of antigen than MagMSN (fig. 2), indicating that MagParV has higher antigen loading capacity. To investigate the antigen release capacity of the broad-spectrum endoplasmic reticulum-targeted magnetic vesicle adjuvant, magParV loaded with antigen was stimulated with alternating magnetic field (400 hz,100 w), after 30min, the antigen release rate of MagParV reached 45%, while MagMSN was only 15% (fig. 2), indicating that MagParV has a higher alternating magnetic field response antigen release capacity. Since MagMSN and MagParV both contain superparamagnetic MnFe 2 O 4 The nanoparticle is therefore capable of generating heat under alternating magnetic field treatment conditions, whereby the heat promotes the release of antigen.
(2) Immunoprotection of infection by anti-candida albicans vaccine
ELISA method detection of serum specific antibody shows that, inoculation of free CaAg antigen only produces lower level IgG, and dilution titer is 50000; the IgG level increases after CaAg antigen binds to CFA or MagMSN, the dilution titer is 100000 ~ 200000, the IgG level produced by binding to MagParV is higher, the dilution titer is >200000, the IgG level is significantly up-regulated after alternating magnetic field treatment (50-500000 hz, 10-5000 w, 1-60 min; optimum parameters are 400hz,100w,10 min), the dilution titer is >800000 (fig. 3). The above results indicate that MagParV under alternating magnetic field treatment can cause the highest level CaAg IgG production.
After systemic infection with candida albicans, all unvaccinated mice died within 4 days, caAg, caag+cfa, caag+magmsn, caag+magmsn+ alternating field vaccinated mice died within 7 days, and caag+magparv vaccinated mice died within 3-10 days, in contrast to which caag+magparv+ alternating field had a strong inhibitory effect on the death of mice, with 70% of mice still surviving on day 20 post-infection (fig. 4). The above results indicate that: the anti-candida albicans vaccine has remarkable protection effect on candida albicans infection and can effectively protect mice from serious candida albicans systemic infection.
Example 5 preparation of endoplasmic reticulum-targeting magnetic vesicle anti-Staphylococcus aureus vaccine
1 test method
(1) Synthesis and characterization of anti-staphylococcus aureus vaccine:
centrifuging Staphylococcus aureus cells cultured overnight at 37deg.C, and re-suspending in sterile water to give a bacterial concentration of 2×10 8 cells/mL. Ultrasonic crushing, wherein ultrasonic conditions are as follows: the sample was ice-bathed, 100W, sonicated for 3s, stopped for 3s, and sonicated 100 times. Centrifuging at 12000rpm for 5min, and obtaining the supernatant as staphylococcus aureus antigen SaAg.
SaAg, magMSN prepared as described in example 4, parV vesicles prepared in example 2 were combined in PBS according to 1:2:1 to obtain the anti-staphylococcus aureus vaccine SaAg+MagParV. The method adopts transmission electron microscope observation, zeta potential analysis, antigen release analysis and other methods to characterize the anti-staphylococcus aureus vaccine.
(2) Anti-infective performance evaluation of anti-staphylococcus aureus vaccine:
balb/c female mice were vaccinated with SaAg+MagParV+ alternating magnetic field (10 μg+25 μg, alternating magnetic field treatment for 10 min) on day-14 and day-7 to complete immunization. Mouse serum was collected on day 0, day 2, day 4, day 8 and day 20, and infected mice were assayed for anti-SaAg IgG levels by ELISA.
2 test results
(1) Characterization of broad-spectrum endoplasmic reticulum targeting magnetic vesicle adjuvants
Same as in example 4.
(2) Immunoprotection of infection by anti-staphylococcus aureus vaccine
ELISA method detection of serum specific antibody shows that, inoculation of free SaAg antigen only produces lower level serum IgG, and dilution titer is less than 100000; the IgG level increased after SaAg bound to CFA or MagMSN, the dilution titer was 100000 ~ 200000, the IgG level produced by binding to MagParV was higher, the dilution titer was >200000, the IgG level was significantly up-regulated after alternating magnetic field treatment, and the dilution titer was >650000 (fig. 5). The above results indicate that MagParV under alternating magnetic field treatment can cause the highest level of SaAg IgG production.
Example 6 preparation of endoplasmic reticulum-targeting magnetic vesicle anti-tumor vaccine
1 test method
(1) Synthesis and characterization of antitumor vaccine:
brain glioma GL261 cells cultured at 37 ℃ for 48 hours were centrifuged, resuspended in sterile water and sonicated. Centrifuging at 5000rpm for 1min, and collecting supernatant as GLAg antigen GL261 of brain glioma. GLAg, magMSN, parV vesicles were taken up in PBS according to 1:2:1 to obtain the glioma vaccine GLAg+MagParV. The anti-tumor vaccine is characterized by adopting methods such as transmission electron microscope observation, zeta potential analysis, antigen release analysis and the like.
(2) Anti-infective performance evaluation of anti-tumor vaccine:
c57 female mice were vaccinated with GLAg+MagParV+ alternating magnetic field (10 μg+25 μg, alternating magnetic field treatment for 10 min) on day-14 and day-7 to complete immunization. Mouse serum was collected on day 0, day 10 and day 20, and infected mice were assayed for anti-GLAg IgG levels by ELISA. On day 30, GL261 cells were inoculated in an amount of 1X 10 in the right armpit of immunized mice 6 Cells/cells. On day 50And taking out the tumor, weighing the tumor, and analyzing the inhibition effect of the anti-tumor vaccine on the tumorigenesis.
2 test results
(1) Characterization of broad-spectrum endoplasmic reticulum targeting magnetic vesicle adjuvants
As in the first embodiment.
(2) Inhibition of tumorigenesis by anti-tumor vaccine
After 20 days of tumor inoculation of each immunized mouse, tumors are weighed, the weights of the free GLAg group and the GLAg+CFA group are respectively 0.89 times and 0.51 times that of the non-immunized control group, and the weights of the GLAg+MagParV+ alternating magnetic field treatment group tumors are only 0.18 times that of the non-immunized control group (figure 6), which shows that the anti-tumor vaccine GLAg+MagParV has a remarkable inhibition effect on tumorigenesis after being combined with an alternating magnetic field.
Although the foregoing embodiments have been described in some, but not all, embodiments of the invention, according to which one can obtain other embodiments without inventiveness, these embodiments are all within the scope of the invention.
Claims (10)
1. An endoplasmic reticulum targeting artificial protein is characterized in that the amino acid sequence is shown as SEQ ID NO.2 from the N end to the C end.
2. The endoplasmic reticulum targeting artificial protein coding gene of claim 1, wherein the nucleotide sequence is shown in SEQ ID NO. 3.
3. A recombinant saccharomyces cerevisiae, which is characterized by taking ura 3-defective saccharomyces cerevisiae as an original cell and comprising a recombinant plasmid; the recombinant plasmid has the coding gene of claim 2 inserted therein.
4. Use of the artificial protein of claim 1 or the coding gene of claim 2 or the recombinant saccharomyces cerevisiae of claim 3 for the preparation of endoplasmic reticulum targeting vesicles.
5. The preparation method of the endoplasmic reticulum targeting vesicle is characterized by comprising the following steps of:
inoculating the recombinant saccharomyces cerevisiae of claim 3 into an SC-Gal medium for a first induction culture;
inoculating the recombinant saccharomyces cerevisiae subjected to the first induction culture to an SC-Gal culture medium without an amino-free yeast nitrogen source, and performing a second induction culture;
centrifuging the culture after the second induction culture, collecting precipitated yeast cells, and extracting protoplasts of the yeast cells to obtain yeast protoplasts;
crushing and ultracentrifugation are sequentially carried out on the yeast protoplast, and sediment is collected to obtain a crude product of the endoplasmic reticulum targeting vesicle;
purifying the crude vesicle product by adopting a nickel column to obtain an endoplasmic reticulum targeting vesicle;
the SC-Gal culture medium takes water as a solvent and comprises the following components in concentration: 6.7g/L of nitrogen source of the yeast without amino group, 20g/L of galactose and 2g/L of mixed amino acid;
the SC-Gal culture medium without the nitrogen source of the amino-free yeast takes water as a solvent and comprises the following components in concentration: galactose 20g/L and mixed amino acid 2g/L;
the mixed amino acid comprises the following components in parts by mass: 2 parts of L-proline, 10 parts of L-leucine, 2 parts of L-valine, 2 parts of L-alanine, 2 parts of L-serine, 2 parts of L-lysine, 2 parts of L-glutamic acid, 2 parts of L-methionine, 2 parts of L-threonine, 2 parts of L-glutamine, 2 parts of L-glycine, 2 parts of L-isoleucine, 2 parts of L-tryptophan, 2 parts of L-aspartic acid, 2 parts of L-tyrosine, 2 parts of L-phenylalanine, 2 parts of inositol, 2 parts of L-cysteine, 2 parts of p-aminobenzoic acid, 2 parts of L-asparagine and 0.5 part of adenine.
6. An endoplasmic reticulum targeting magnetic vesicle, which is characterized by comprising magnetic mesoporous silicon nano-particles and endoplasmic reticulum targeting vesicles coated in pore channels of the magnetic mesoporous silicon nano-particles, wherein the endoplasmic reticulum targeting vesicles are prepared by the preparation method of claim 5.
7. The endoplasmic reticulum-targeted magnetic vesicle of claim 6, wherein the inner core of the magnetic mesoporous silicon nanoparticle is MnFe 2 O 4 A nanoparticle; the shell of the magnetic mesoporous silicon nanoparticle is mesoporous silicon dioxide;
the preparation method of the magnetic mesoporous silicon nanoparticle comprises the following steps:
MnFe is mixed with 2 O 4 Suspending in an organic solvent to obtain a suspension;
firstly mixing the suspension, the pore-forming agent and water, and removing the organic solvent to obtain a first mixed solution;
mixing the first mixed solution, water, methanol and ethyl acetate for the second time to obtain a second mixed solution;
thirdly mixing the second mixed solution, an ammonium hydroxide solution, tetraethoxysilane and 3-aminopropyl triethoxysilane to obtain a third mixed solution;
and centrifuging the third mixed solution, collecting precipitate, washing the precipitate, and removing the pore-forming agent to obtain the magnetic mesoporous silicon nanoparticle.
8. Use of an endoplasmic reticulum-targeted magnetic vesicle according to claim 6 or 7 for the preparation of an immunoadjuvant.
9. A vaccine comprising magnetic mesoporous silicon nanoparticles, and endoplasmic reticulum targeting vesicles and antigens coated in the pores of the magnetic mesoporous silicon nanoparticles, wherein the endoplasmic reticulum targeting vesicles are prepared by the preparation method of claim 5.
10. The vaccine of claim 9, wherein the antigen comprises a pathogenic antigen or a tumor antigen.
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CN109136245A (en) * | 2010-09-28 | 2019-01-04 | 圣母大学 | Chimeric spider silk and application thereof |
US20160331686A1 (en) * | 2015-05-12 | 2016-11-17 | Clsn Laboratories, Inc. | Compositions and Methods for Yeast Extracellular Vesicles as Delivery Systems |
CN112074298A (en) * | 2018-01-18 | 2020-12-11 | 马克斯·普朗克科学促进协会 | Langerin+Cell targeting |
CN112773901A (en) * | 2021-01-29 | 2021-05-11 | 国家纳米科学中心 | Bacterial outer membrane vesicle carrier and preparation method and application thereof |
WO2023015213A1 (en) * | 2021-08-03 | 2023-02-09 | Spotlight Therapeutics | Ptpn2 specific guide rnas and uses thereof |
CN114939159A (en) * | 2022-05-13 | 2022-08-26 | 浙江大学 | Construction and application of virus antigen and adjuvant-loaded multistage targeting vector |
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