CN115895854A - Multi-card compatible molecular diagnostic system - Google Patents

Abstract

The invention provides a multi-card compatible molecular diagnostic system, which comprises a nucleic acid extracting and liquid adding system, a sample introduction system, a nucleic acid amplification system and a hand grip and detection system, wherein the nucleic acid extracting and liquid adding system comprises a molecular detection plate; the sample introduction system comprises a molecular detection card, the molecular detection card comprises a single-step PCR detection card and a nested PCR detection card, the nested PCR detection card comprises a first liquid suction port, a second liquid suction port, a third liquid suction port and a molecular detection card cavity, the first liquid suction port, the second liquid suction port and the third liquid suction port are respectively connected with the molecular detection card cavity, the nested PCR detection card further comprises an undeformable card body, and the molecular detection card cavity is arranged in the undeformable card body. The function of testing both single-item and multi-item, single-step amplification and two-step amplification is realized in the same diagnostic system.

Description

Multi-card compatible molecular diagnostic system

Technical Field

The invention relates to the field of in-vitro diagnostic molecular detection instruments, in particular to a molecular detection system which is compatible with a single item and multiple items, single-step amplification and two-step amplification.

Background

The present in vitro diagnosis molecular detector is mainly used for molecular diagnosis, which is a technology for diagnosing by detecting the structure or expression level change of genetic material in a patient body by applying a molecular biology method, and the molecular diagnosis is a main method for predicting diagnosis, which can diagnose individual genetic diseases and antenatal diagnosis, and mainly refers to the detection of various structural proteins, enzymes, antigen antibodies and immune activity molecular genes related to the coding diseases.

The existing molecular diagnosis system is one single visual test, one is a multi-item visual test, and no system is an instrument and a system which can test a single item and also test multiple items (more than 6).

The existing molecular system is either single-step amplification or two-step amplification, and a detection system compatible with the two is not provided.

The existing automatic large-flux analysis instrument can not work in the common laboratory environment due to the pollution control problem, and the pollution problem needs to be solved so as to be used in the common laboratory environment.

The problem of randomness (unplanned property) of hospital projects is solved, one project can be detected on the same machine, and a plurality of projects can also be detected.

The method solves the problem of unplanned precision requirements, supports single-step amplification and secondary amplification of some projects with high precision requirements, and supports both nested PCR and common PCR.

Therefore, a new multi-card compatible molecular diagnostic system is needed to be provided, which is compatible with the above requirements.

Disclosure of Invention

The invention provides a multi-card compatible molecular diagnostic system, which at least solves the technical problem that the prior art can not realize the test of both single items and multiple items, and can realize single-step amplification and two-step amplification in the same diagnostic system.

The invention provides a multi-card compatible molecular diagnostic system, which comprises a nucleic acid extracting and liquid adding system, a sample feeding system, a nucleic acid amplification system, a hand grip and a detection system, wherein the sample adding system is positioned above the sample feeding system;

the sample introduction system comprises a molecular detection card, the molecular detection card comprises a single-step PCR detection card and a nested PCR detection card, the nested PCR detection card comprises a first liquid suction port, a second liquid suction port, a third liquid suction port and a molecular detection card cavity, the first liquid suction port, the second liquid suction port and the third liquid suction port are respectively connected with the molecular detection card cavity, the nested PCR detection card further comprises an undeformable card body, and the molecular detection card cavity is arranged in the undeformable card body.

Optionally, the single-step PCR detection card is a single-step single-item PCR detection card and/or a single-step multi-item PCR detection card, and the molecular detection plate includes a single-tube reagent card.

Optionally, the nested PCR detection card further includes an undeformable card body, which means that the nested PCR detection card has an undeformable outer profile card body, and the molecular detection card cavity is disposed in the undeformable card body, which means that the molecular detection card cavity is disposed in the undeformable outer profile card body, and when the gripper of the gripper and the detection system grips the nested PCR detection card for transportation, the molecular detection card cavity does not deform.

Optionally, when the molecular detection is required, the first liquid suction port and the second liquid suction port of the sample to be detected including the molecular detection card are evacuated to a negative pressure.

Optionally, the molecule detection plate is provided with a dovetail groove, the dovetail groove is located at the tail of the molecule detection plate, the molecule detection plate further comprises a cover plate, the cover plate is connected with the dovetail groove in a matched manner in two stages, the two stages of connection in the matched manner comprise an uncovering connection and a closing connection, the cover plate is changed from the uncovering connection to the closing connection under the action of external force, and the dovetail groove and the molecule detection plate are fixedly connected into an integrated structure.

Optionally, the cavity of the molecular detection card comprises a liquid storage cavity, a mixing cavity, a first amplification cavity and a second amplification cavity, the first liquid suction port is communicated with the first amplification cavity, the second liquid suction port is communicated with the liquid storage cavity, the liquid storage cavity is connected with the mixing cavity, the mixing cavity is connected with the first amplification cavity, the mixing cavity is connected with the second amplification cavity, and the third liquid suction port is communicated with the second amplification cavity.

Optionally, the liquid storage chamber and/or the mixing chamber and/or the first amplification chamber are deformable chambers.

Optionally, a pressing shell is disposed on the non-deformable card body, the pressing shell is disposed outside the liquid storage cavity and/or the first amplification cavity, the pressing shell is stressed and then acts on the liquid storage cavity and/or the first amplification cavity to deform the liquid storage cavity and/or the first amplification cavity, the number of the mixing cavities is greater than 1, and the mixing cavities with the number greater than 1 are sequentially connected in series.

Optionally, the multi-card compatible molecular diagnostic system is in a negative pressure state.

Optionally, the molecular diagnostic system is a fully-automatic integrated multi-card compatible molecular diagnostic system that evacuates the molecular detection card when the molecular detection is required.

PCR is an abbreviation of English Polymerase Chain Reaction, which is explained in Chinese as Polymerase Chain Reaction, and is a molecular biology technique for amplifying and amplifying specific DNA fragments, which can be regarded as special DNA replication in vitro.

The multi-card compatible molecular diagnosis system provided by the invention solves the technical problem of randomness (unplanned) of hospital projects, can detect one project or a plurality of projects on the same machine, and can amplify in a single step or two steps.

The method solves the unplanned technical problem of precision requirement, supports single-step amplification and secondary amplification of some projects with high precision requirement, and supports both nested PCR and common PCR.

The nested PCR technology can eliminate the interference of other nonspecific pathogenic bacteria nucleic acid substances to the maximum extent, and meanwhile, the two-step PCR amplification can also ensure that the pathogenic bacteria can be quickly detected under the condition of extremely low content of the pathogenic bacteria, so that the detection sensitivity is improved.

For some samples with high abundance, the cost can be reduced and the detection time can be saved by adopting the common PCR.

The molecular diagnosis system provided by the technical scheme of the invention is a full-automatic integrated multi-card compatible molecular diagnosis system for vacuumizing the molecular detection card when the molecular detection is required, so that the pollution problem is solved, and the automatic molecular diagnosis instrument can be used in the common laboratory environment.

Consumable and sample can automatic introduction and withdraw a kind, and the machine can cascade on a large scale, realizes that big flux many varieties detect simultaneously.

Drawings

The above and other objects, features and advantages of exemplary embodiments of the present invention will become readily apparent from the following detailed description, which proceeds with reference to the accompanying drawings. Several embodiments of the invention are illustrated by way of example, and not by way of limitation, in the figures of the accompanying drawings and in which:

FIG. 1 is a schematic diagram of an alternative multi-card compatible molecular diagnostic system in accordance with embodiments of the present invention;

FIG. 2 is a schematic structural diagram of an alternative sample injection system according to an embodiment of the present invention;

FIG. 3 is a schematic diagram of an alternative single-step PCR detection card according to an embodiment of the present invention;

FIG. 4 is a schematic view of an alternative nested PCR detection card provided in an embodiment of the present invention;

FIG. 5 is a schematic view of an alternative molecular assay plate according to an embodiment of the present invention;

FIG. 6 is an enlarged schematic view of another alternative structure of a nested PCR detection card included in an alternative molecular detection card according to an embodiment of the present invention;

FIG. 7 is a perspective view of FIG. 6;

FIG. 7-1 is a schematic view of the partially exploded structure of FIG. 6;

FIG. 8 is a schematic diagram of an alternative nucleic acid extraction and priming system according to embodiments of the present invention;

fig. 9 is a schematic diagram of an alternative heat-sealing process of a sample injection system according to an embodiment of the present invention.

The following detailed description is intended to further illustrate but not limit the invention, the following example being only one preferred embodiment of the invention.

Detailed Description

The principles and spirit of the present invention will be described with reference to several exemplary embodiments. It is understood that these embodiments are given solely for the purpose of enabling those skilled in the art to better understand and to practice the invention, and are not intended to limit the scope of the invention in any way. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.

As shown in fig. 1, the present invention provides a multi-card compatible molecular diagnostic system, which comprises a nucleic acid extraction and liquid adding system 1, a sample adding system 2, a sample introduction system 3, a nucleic acid amplification system 4, and a gripper and detection system 5, wherein the sample introduction system 2 is located above the sample introduction system 3, the gripper and detection system 5 is located above the nucleic acid amplification system 4, the nucleic acid extraction and liquid adding system 1, the sample introduction system 3, and the nucleic acid amplification system 4 are adjacent in sequence, the nucleic acid extraction and liquid adding system 1 comprises a molecular detection plate, such as a single-tube reagent card, for rapid detection of a detection item requiring only a single tube;

as shown in fig. 2, fig. 2 is an enlarged schematic view of the sample feeding system 3, the sample feeding system 3 includes a molecular detection card, the molecular detection card includes a single-step PCR detection card as shown in fig. 3 and a nested PCR detection card as shown in fig. 4, the nested PCR detection card 34 includes a first liquid suction port 341, a second liquid suction port 342, a third liquid suction port 347 and a molecular detection card cavity, the first liquid suction port 341, the second liquid suction port 342 and the third liquid suction port 347 are respectively connected to the molecular detection card cavity, the nested PCR detection card 34 further includes a non-deformable card body, and the molecular detection card cavity is disposed in the non-deformable card body.

Further, the single-step PCR detection card is a single-step single-item PCR detection card and/or a single-step multi-item PCR detection card, and the single-step multi-item PCR detection card is shown in fig. 3.

Further, the nested PCR detection card 34 further includes an undeformable card body, which means that the nested PCR detection card 34 has an undeformable outer profile card body 348, the molecular detection card cavity is disposed in the undeformable card body, which means that the molecular detection card cavity is disposed in the undeformable outer profile card body, and when the gripper of the gripper and detection system 5 grips the nested PCR detection card 34 for transportation, the molecular detection card cavity does not deform, for example, the detection card plate of the nested PCR detection card shown in fig. 4 is made of a hard material, and the circumference of the detection card can be gripped by the gripper and the gripper of the detection system 5 to complete the full-automatic mechanical transportation.

Further, when the molecular detection is required, the first and second pipetting holes 341 and 342 of the sample to be detected including the molecular detection card are evacuated to negative pressure, and at this time, the negative pressure is evacuated in real time for real-time detection, so that almost zero pollution and low cost are achieved.

Further, as shown in fig. 5, fig. 5 is an enlarged schematic view of the molecule detecting plate 11, the molecule detecting plate 11 is provided with a dovetail groove 112, the dovetail groove 112 is located at the tail of the molecule detecting plate 11, the molecule detecting plate 11 further comprises a cover plate 111, the cover plate 111 is in fit connection with the dovetail groove 112 in two stages, the two stages of fit connection comprise an open cover connection and a close cover connection, the cover plate 111 is changed from the open cover connection to the close cover connection under the action of an external force, the dovetail groove 112 is fixedly connected with the molecule detecting plate 11 into an integrated structure, the integrated structure provides a possibility of full-automatic integration, because the operations of opening and closing the cover do not need to be manually performed, for a disposable molecule detecting plate, the initial state can be an open cover state, and after sample application, the system, such as a mechanical baffle, applies an external force to the cover plate 111 to change from the open cover connection to the close cover connection, which can replace manual sample application and lid operation.

Further, as shown in FIG. 6, FIG. 6 is an enlarged schematic view of another structure of the nested PCR detection card included in the molecular detection card, the cavity of the molecular detection card includes a liquid storage cavity 343, a mixing cavity 344, a first amplification cavity 345 and a second amplification cavity 346, the first pipette port 341 communicates with the first amplification cavity 345, the second pipette port 342 communicates with the liquid storage cavity 343, the liquid storage cavity 343 communicates with the mixing cavity 344, the mixing cavity 344 communicates with the first amplification cavity 345, the mixing cavity 344 communicates with the second amplification cavity 346, and the third pipette port 347 communicates with the second amplification cavity 346.

Further, the liquid storage chamber 343, the mixing chamber 344, and the first amplification chamber 345 shown in fig. 6 and 7-1 are deformable chambers, that is, the liquid storage chamber 343, the mixing chamber 344, and the first amplification chamber 345 may deform to squeeze out the liquid in the chambers when receiving an external force, so that the liquid flows into the next chamber, and the external force may be generated from an applied force generated by a hard object such as a robot arm or a baffle of the system.

Further, as shown in fig. 6, 7 and 7-1, fig. 7 is a schematic perspective view of fig. 6, fig. 7-1 is a schematic partial explosion structure of fig. 6, the non-deformable card body is provided with a first pressing shell 3431 and a second pressing shell 3451, the first pressing shell 3431 and the second pressing shell 3451 are disposed outside the liquid storage chamber 343 and the first amplification chamber 345 shown in fig. 6, the first pressing shell 3431 and the second pressing shell 3451 are stressed and act on the liquid storage chamber 343 and the first amplification chamber 345 shown in fig. 6, so that the liquid storage chamber 343 and the first amplification chamber 345 are deformed, and thus the fluid in the chambers flows to generate an impulsive force to break the state that the two films are adhered to each other to block the communication between the two chambers, the number of the mixing chambers 344 is greater than 1, for example, may be 8 or more, the mixing chambers 344 are sequentially connected in series, which may function as a fully mixing, a second preparation for nested PCR is made, and the origin of adhesion is shown in fig. 7-349.

Furthermore, the multi-card compatible molecular diagnosis system is in a negative pressure state and is provided with a negative pressure anti-pollution system.

Furthermore, the molecular diagnosis system is a fully-automatic integrated multi-card compatible molecular diagnosis system which vacuumizes the molecular detection card when the molecular detection is needed.

Furthermore, the whole multi-card compatible molecular diagnosis system has the functions of nucleic acid extraction, reagent system addition, sample suction to consumables, nucleic acid amplification and fluorescence detection.

Further, the liquid storage chamber 343 and/or the mixing chamber 344 and/or the first amplification chamber 345 are soft air chamber bodies, and the molecular detection card is a molecular detection card for nested PCR multi-item detection.

The working principle of the whole machine is as follows:

FIG. 8 is an enlarged view of the nucleic acid extracting and feeding system 1, and FIG. 9 is a schematic view of an alternative heat-sealing process of a sample injection system according to an embodiment of the present invention;

adding a reagent-integrated consumable material, such as the molecular detection plate 11, into the nucleic acid extraction and filling system 1, placing the molecular detection card, such as the nested PCR detection card 34, into the sample holder of the sample injection system 3, starting the machine, adding the sample into the nucleic acid extraction hole by the machine, extracting the nucleic acid, adding the system liquid into the cuvette by the multi-head filling system, adding the nucleic acid extract and the system liquid mixture into the sample tube 33 by the sample injection system 2, adding the PCR-II reaction liquid into the other sample tube, transporting the sample holder 32 to the vacuum cavity 35 by the transport ship 31 of the sample injection system 3, lowering the vacuum cavity 35 to the sealing ring of the transport ship 31 to form a sealed cavity vacuum chamber, pumping the vacuum system to generate negative pressure, lowering the first heat gun 36 to the third liquid suction port 347 of the molecular detection card, heat-sealing the third liquid suction port 347, raising the vacuum cavity 35, communicating the transport ship 31 with the atmosphere, and lowering the liquid in the sample tube 33 to the second liquid suction port 341 and heat-sealing the second liquid suction port 341 and the second liquid suction port 342. The transport ship 31 transports the sample rack 32 to the amplification site, the gripper of the gripper and detection system 5 places the molecular detection card 34 to the nucleic acid amplification site of the nucleic acid amplification system 4, the nucleic acid amplification supports two-step amplification, adapting to nested PCR, and the gripper is provided with a detection system for detecting fluorescent signals on each consumable.

In the above-mentioned nucleic acid extraction and liquid feeding system 1, the improvement point of the core lies in that the above-mentioned molecular detection board 11 of reagent integration consumptive material has been adopted, above-mentioned molecular detection board 11 is by the reaction cup, the reaction storehouse, the magnetic bead position, the reagent position, the magnetic sleeve, the sample tube position, tip head position and apron are constituteed, wherein the biggest improvement point is just as above-mentioned, dovetail groove and apron complex structure has been adopted, for disposable molecular detection board, initial condition can be for uncapping the state, add the back by the system, for example mechanical baffle or similar motion for above-mentioned apron 111 exert exogenic action by the above-mentioned uncapping connection becomes the above-mentioned connection of closing, can replace artifical application of sample closing lid operation, thereby bring the detection of single-barreled reagent card into entire system and realized the full-automatic possibility.

Inserting a reagent integrated consumable, placing a sample tube into a sample position of the consumable, starting detection, extracting nucleic acid by an instrument according to a nucleic acid extraction flow, and adding a reagent system into a reaction tube by a multi-head liquid adding system.

The sample introduction system 3 comprises a sample rack, a sample tube, a molecule detection consumable, a transportation system, a heat gun, a vacuum cavity and a vacuum pumping system, wherein a spout-shaped avoiding structure is arranged on the sample tube, collision with a sample suction tube of the consumable is avoided during sample addition, a pipette on the molecule detection consumable is inserted into the sample tube, liquid is always higher than a pipette port, the transportation ship transports the sample rack, the sample tube and the molecule detection consumable on the sample rack and the sample tube and the molecule detection consumable on the sample rack to a vacuum pumping vacancy, the vacuum cavity is tightly pressed by a sealing ring with the transportation ship under the action of a movement mechanism, the vacuum system vacuumizes the cavity, after all cavities in the molecule consumable are vacuumized, the third pipette port 347 of a PCR-II reaction cavity (the second amplification cavity 346) is thermally sealed by the first heat gun 36, the vacuum cavity is opened, the sample and the PCR-II reaction liquid are sucked into the corresponding cavities, the transportation system transports the sample rack to a thermal sealing position, and the second heat gun 37 transports the first pipette port 341 and the second pipette port 342 to be thermally sealed, so that the transportation ship 31 continuously moves to the PCR amplification position.

The sampling tube is inserted into the sample tube 33, the consumables and the sample tube are transported to the vacuum chamber for vacuum pumping, the third liquid suction port 347 is heat sealed by the first heat gun 36, then the vacuum chamber is opened to be communicated with the atmosphere, the sample flows into each PCR-I reaction chamber (the first amplification chamber 345) from the first liquid suction port 341, and the PCR-II reaction solution flows into the PCR-II liquid storage chamber (the liquid storage chamber 343) from the second liquid suction port 342. The second heat gun 37 heat-seals the first liquid suction port 341 and the second liquid suction port 342. The PCR-I reaction cavity is provided with freeze-dried probes, primers, taq polymerase and 4 DNTPs. Then PCR amplification is performed. After the first-step amplification is completed, a PCR-II reaction liquid valve V1 (a first reaction liquid valve V1) and a valve V2 (a second amplification valve V2) for the first PCR-I amplification in the consumable are opened, the two liquids are mixed in the mixing cavity, the two valves are closed, a third valve V3 (a third valve V3) is opened, the mixture flows into a PCR-II reaction chamber (a second amplification chamber 346), a freeze-dried probe, a nested primer, taq polymerase and 4 DNTPs are arranged in the reaction chamber, and after the third valve V3 is closed, PCR amplification and fluorescence detection are carried out.

The detection position can be compatible with a single-item detection tube and a multi-item detection tube, the item detection supports single-step amplification and two-step amplification, the nested PCR technology and the common PCR technology are compatible, a complete anti-pollution system is embodied in that nucleic acid extraction and reagents are sucked into a reaction consumable material under negative pressure, a nucleic acid extraction bin and a sample bin are provided with closed covers, a nucleic acid transfer cup is added with a reagent for dissolving nucleic acid, the whole machine is in a negative pressure state, a filter membrane is used for filtering, and an ultraviolet sterilization system is used.

An anti-pollution system: the integrated reagent strip is provided with a mechanism for sealing a sample and a nucleic acid extraction cavity, a sample tube can be timely thrown into the solid-liquid garbage can, PCR amplification consumables adopt heat sealing and negative pressure sample introduction, the top of the whole machine is provided with a negative pressure system and a filter membrane, and the top of the whole machine is also provided with an ultraviolet sterilization system.

The core improvement of the technical scheme of the embodiment of the invention is that negative pressure sample suction in sample adding is realized, the consumable part is less, the pollution is reduced, the automation and less pollution can be better realized by the molecular detection plate with the dovetail groove cover structure, the vacuum-pumping sample adding detection can be fully automatically realized when molecular diagnosis is needed, and the full-automatic partial sharing function of micro-fluidic technology and nucleic acid extraction is realized through multiple projects, so that the full automation of nested PCR is realized for the first time.

Claims (10)

1. The multi-card compatible molecular diagnosis system is characterized by comprising a nucleic acid extracting and liquid adding system (1), a sample adding system (2), a sample introduction system (3), a nucleic acid amplification system (4) and a gripper and detection system (5), wherein the sample adding system (2) is positioned above the sample introduction system (3), the gripper and detection system (5) is positioned above the nucleic acid amplification system (4), the nucleic acid extracting and liquid adding system (1), the sample introduction system (3) and the nucleic acid amplification system (4) are sequentially adjacent, and the nucleic acid extracting and liquid adding system (1) comprises a molecular detection plate (11);

the sample introduction system (3) comprises a molecular detection card, the molecular detection card comprises a single-step PCR detection card and a nested PCR detection card, the nested PCR detection card (34) comprises a first liquid suction port (341), a second liquid suction port (342), a third liquid suction port (347) and a molecular detection card cavity, the first liquid suction port (341), the second liquid suction port (342) and the third liquid suction port (347) are respectively connected with the molecular detection card cavity, the nested PCR detection card (34) further comprises a non-deformable card body, and the molecular detection card cavity is arranged in the non-deformable card body.

2. The multi-card compatible molecular diagnostic system according to claim 1, wherein the single-step PCR test card is a single-step single-item PCR test card and/or a single-step multi-item PCR test card, and the molecular test board (11) comprises a single-tube reagent card.

3. The multi-card compatible molecular diagnostic system of claim 1, wherein the nested PCR detection card (34) further comprises a non-deformable card body, which means that the nested PCR detection card (34) has a non-deformable outer profile card body, and the molecular detection card cavity is disposed in the non-deformable card body, which means that the molecular detection card cavity is disposed in the non-deformable outer profile card body, and when the gripper of the gripper and detection system (5) grips the nested PCR detection card (34) for transportation, the molecular detection card cavity is not deformed.

4. The multi-card compatible molecular diagnostic system according to claim 1, wherein when the molecular test is required, the first (341) and second (342) ports containing the sample to be tested of the molecular test card are evacuated to a negative pressure.

5. The multi-card compatible molecular diagnostic system according to claim 1, wherein a dovetail groove (112) is provided on the molecular detection plate (11), the dovetail groove (112) is located at the tail of the molecular detection plate (11), the molecular detection plate (11) further comprises a cover plate (111), the cover plate (111) is in fit connection with the dovetail groove (112), the cover plate (111) and the dovetail groove (112) are in fit connection in two stages, the two stages of fit connection comprises an uncovering connection and a closing connection, the cover plate (111) is changed from the uncovering connection to the closing connection under the action of external force, and the dovetail groove (112) and the molecular detection plate (11) are fixedly connected into an integral structure.

6. The system of claim 1, wherein the molecular test card chamber comprises a liquid storage chamber (343), a mixing chamber (344), a first amplification chamber (345), and a second amplification chamber (346), the first fluid suction port (341) is in communication with the first amplification chamber (345), the second fluid suction port (342) is in communication with the liquid storage chamber (343), the liquid storage chamber (343) is connected to the mixing chamber (344), the mixing chamber (344) is connected to the first amplification chamber (345), the mixing chamber (344) is connected to the second amplification chamber (346), and the third fluid suction port (347) is in communication with the second amplification chamber (346).

7. The multi-card compatible molecular diagnostic system of claim 6, wherein the liquid storage chamber (343) and/or the mixing chamber (344) and/or the first amplification chamber (345) are deformable chambers.

8. The multi-card compatible molecular diagnostic system according to claim 6, wherein a pressing shell is disposed on the non-deformable card body, the pressing shell is disposed outside the liquid storage chamber (343) and/or the first amplification chamber (345), the pressing shell acts on the liquid storage chamber (343) and/or the first amplification chamber (345) after being stressed to deform the liquid storage chamber (343) and/or the first amplification chamber (345), the number of the mixing chambers (344) is greater than 1, and the mixing chambers (344) with the number greater than 1 are connected in series in sequence.

9. The multi-card compatible molecular diagnostic system of claim 1, wherein the multi-card compatible molecular diagnostic system is in a negative pressure state.

10. The multi-card compatible molecular diagnostic system according to any one of claims 1 to 9, wherein the molecular diagnostic system is a fully automated integrated multi-card compatible molecular diagnostic system that evacuates the molecular test card when a molecular test is required.

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