CN115777526A - Breeding method of fine grain fragrance type high-quality temperature-sensitive rice genic male sterile line - Google Patents
Breeding method of fine grain fragrance type high-quality temperature-sensitive rice genic male sterile line Download PDFInfo
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Abstract
The invention provides a breeding method of a fine grain odor type high-quality temperature-sensitive rice genic male sterile line, belonging to the technical field of rice breeding, and the method comprises the following steps: selecting two-line sterile line deep 08S as female parent, hybridizing with self-bred strain B32028 to obtain seed, and transplanting early rice to form F 1 Late rice transplanting F 2 Generation, early rice single plant transplanting formation F 3 The generation line is formed by sowing seeds of individual plants in the late rice of the same year and transplanting the individual plants to form F 4 Generation line, planting early rice in the next year F 5 Generation sterile line, late rice planting in Nanning F 6 Generation line, planting early rice in the next year F 7 Generation line, planting late rice in the same year to form F 8 Generation line, early rice planting in the next year forms F 9 Generation line, planting late rice in the same year F 10 Generation line, harvest F 10 The generation seed is the research result, fine grain fragrance type high-quality temperature-sensitive genic male sterile line Yi Xiang S. The high-quality thermo-sensitive genic male sterile rice cultivated by the invention integrates main excellent genetic components such as good leaf morphology, strong tillering capacity, strong combining ability and the like of a deep 08S plant, and has the function of protectingThe holding material has the advantages of long and thin particle shape, fragrance, good taste and the like.
Description
Technical Field
The invention relates to the technical field of rice breeding, in particular to a breeding method of a fine grain fragrance type high-quality temperature-sensitive rice genic male sterile line.
Background
The southern China rice growing region in Guangxi province has the advantages of unique light-temperature ecological resources and rich high-quality conventional rice germplasm resources, and the achievement is remarkable in breeding of high-quality three-line hybrid rice varieties, such as the breeding of high-quality sterile line 'autumn A' and autumn-quality series varieties of rice, the development of late indica high-quality hybrid rice in southern China is powerfully promoted, and the quality of the three-line hybrid rice breeding is remarkably improved; however, there has been no major technical breakthrough in the field of exploring and breeding the fine grain odor type high-quality photo-thermo-sensitive genic male sterile line and the high-end high-quality two-line hybrid rice for a long time, and the fine grain, odor type, high-quality, lodging-resistant thermo-sensitive genic male sterile line and the hybrid rice thereof are relatively short. Therefore, a breeding method of the fine grain fragrance type high-quality thermo-sensitive rice nuclear sterile line rice needs to be researched.
Disclosure of Invention
The invention aims to provide a breeding method of a fine grain fragrance type high-quality thermo-sensitive rice genic male sterile line, and solves the technical problem that the high-quality photo-thermo-sensitive rice genic male sterile line cannot be cultured in the prior art.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
the breeding method of the fine grain odor type high-quality temperature-sensitive rice genic male sterile line comprises the following steps:
step 1: selecting a two-line sterile line deep 08S as a female parent, and hybridizing the female parent with a self-bred strain B32028 to obtain seeds;
and 2, step: carrying out early rice transplanting on the seeds obtained in the step 1 to form F 1 Mixed harvest at maturity F 1 Replacing seeds;
and step 3: late rice transplanting F 2 Generation and harvest F 2 Seed generation;
and 4, step 4: early rice single plant transplanting formation F 3 Generation line and harvest F 3 Seed generation, sowing seeds of individual plants in the late rice of the year, and transplanting the individual plants to form F 4 Generation line, harvest F 4 Seed generation;
and 5: planting early rice in the next year F 5 Generation sterile line, harvest F 5 Generation of seeds, late rice planting in Nanning F 6 Generation line, harvest F 6 Seed generation;
step 6: planting early rice in the next year F 7 Generation line, harvest F 7 Generation seed, planting late rice in the same year to form F 8 Generation line, harvest F 8 Seed generation;
and 7: early rice planting formation F in the next year 9 Generation line, harvest F 9 Generation seed, planting late rice in the same year F 10 Generation line, harvest F 10 The generation seeds are the fine grain fragrant high-quality thermo-sensitive rice genic male sterile line which is the result of the research.
Further, the specific process of step 1 is: two-line sterile line deep 08S with good plant leaf morphology, strong tillering capacity and low transformation critical temperature is selected as a female parent and hybridized and improved with a high-quality maintaining intermediate material B32028 with excellent self-bred plant leaf morphology, and 130-150 seeds are harvested.
Further, the specific process of step 2 is: early rice transplanting formation F 1 Generation 100, hybrid progeny shows good leaf morphology, big ear, slender grain, strong heterosis, mixed harvest F when mature 1 500 g of seed substitute.
Further, the specific process of step 3 is: same year late rice transplanting F 2 Separating 5000 plants in generation, heading in autumn, selecting 63 plants with iodine failure as main component and thorough abortion according to plant leaf morphology, tillering power, ear grain structure, flowering habit, stigma development degree and target character performance of grain type by microscopic examination of pollen grain abortion performanceSterile single plants are cut and regenerated, selfing is carried out under the condition of short days in late autumn, and F is obtained 2 And (5) seed generation.
Further, the specific process of step 4 is: early rice single plant is transplanted to form 63F 3 Selecting 10 plants of 8 plants in the stage of young ear differentiation 4-5, transferring into a cold water irrigation treatment pool, performing circulation irrigation treatment for 12 days by using 22 ℃ cold water, transferring, selfing and fructifying, selecting and harvesting 35 single plants with low fructification rate and long and thin grains, sowing 35 single plants of late rice in the same year, and transplanting the single plants to form F 4 And (3) 35 lines of generation, optimally selecting 20 target single plants of 6 lines in the young ear differentiation period of 4-5, transferring into a cold irrigation pool for cold water treatment and transformation, bagging, selfing and fructifying, and selecting and harvesting 23 long and thin single plant seeds with low selfing fructification rate and long grains.
Further, the specific process of step 5 is: early rice planting in Nanning F 5 Selecting 40 single plants of 5 target plants in 4-5 stages of young ear differentiation for cold water irrigation transformation, bagging, selfing and fructifying, selecting 19 long and thin single plants of grain type for harvesting seeds when the seeds are mature, selecting L61345 and L61347 plant strains and a plurality of high-quality restorer lines for test cross test when the sterile plant strains are ear-pulled, and planting F in late rice 6 Selecting 4 target plants from 19 plants, removing impurities and inferior plants, transplanting half of the plants to carry out cold water irrigation treatment and transformation, isolating, selfing and fructification, selecting 17 target single plants to singly harvest when the plants are mature, mixing the rest plants according to the plants, selecting the other half of the plants to carry out wide test cross with a self-selected high-quality restorer line, planting test cross tests in the same period, and preferably selecting a combined retest test with obvious hybrid vigor.
Further, the specific process of step 6 is: planting early rice in the next year F 7 Selecting two lines with the serial numbers of L71636 and L71638 with stable and consistent agronomic characters in the young ear differentiation 4-5 period, removing impurities and inferior, transferring the parts into a cold irrigation pool for cold water irrigation transformation, isolating, selfing and fructifying, selectively collecting 21 target individual seeds when the seeds are mature, mixing the rest according to the lines, performing plant row comparison and yield comparison tests when testing and matching contracts, selecting test cross combinations with obvious hybrid advantages for small test seed production, and forming F test seed production when the late rice is planted in the same year 8 Generating 21 strains, and selectingAnd (3) removing impurities and inferior parts of the strain with the medium number of L72679, transferring the removed part into a cold irrigation pool for cold water irrigation treatment transformation, isolating self-fertilization and fructification, selectively harvesting 25 target individual plant seeds during maturation, mixing the rest seeds, arranging seedling heads of the late rice in the same year to be combined at the same time, testing a plurality of points, and selecting strong and excellent combination to test seed production according to test performances.
Further, the specific process of step 7 is: early rice planting formation F in the next year 9 Selecting 25 strains in generation, selecting the strain with the number of L81690 in the stage of young ear differentiation of 4-5, removing inferior parts, partially transferring into a cold irrigation pool for cold water irrigation treatment and transformation, isolating, selfing and fructifying, selectively harvesting 17 target individual seeds when the strain is mature, mixing the rest, arranging the combination of seedling heads at a plurality of tests, and testing and preparing seeds in spring season, wherein F is planted in late rice in the same year 10 Generating 17 strains, wherein the 17 sterile strains formed have no obvious difference among field expression strains, stable intra-strain population and consistent agronomic performance, comprehensively evaluating and selecting the serial number L82555 as a selected strain, and then carrying out roguing, cold irrigation treatment transformation, isolation selfing and fructification on the selected strain, after selecting and collecting a plurality of single plant seeds, mixing and harvesting the single plant seeds to obtain the core seed of the fine grain fragrance type high-quality thermosensitive rice nuclear sterile line, simultaneously arranging a seedling head combination in a plurality of dry-spot test tests in different areas, and simultaneously carrying out generative propagation and new combination test seed production.
Due to the adoption of the technical scheme, the invention has the following beneficial effects:
the fine grain fragrance type high-quality temperature-sensitive rice sterile line cultivated by the invention integrates main excellent genetic components such as good leaf shape, strong tillering force, strong combining ability and the like of deep 08S plants, has the advantages of keeping the grain shape of the material slender and fragrant, strong lodging resistance of a series of hybrid rice matched with a self-cultivating high-quality restorer line, good rice taste quality and the like, has obvious differences with the original parent in 7 characters such as green degree of the leaf with two leaves with one plant, the longest length of the ear, thousand grain weight, grain width, brown rice fragrance and the like, belongs to different varieties, and is compared with similar varieties of deep 08S: the green degree of the leaves with two leaves, the number of ears of a single plant, the longest length of the ears, the thousand grain weight of grains, the width of grains, the shape of grains, the width of brown rice, the shape of brown rice, the fragrance of brown rice and other 9 shapes have obvious differences.
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FIG. 1 is a graph of the cultivation system of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail below with reference to the accompanying drawings by way of examples of preferred embodiments. However, it should be noted that the numerous details set forth in the description are merely intended to provide a thorough understanding of one or more aspects of the present invention, even though such aspects of the invention may be practiced without these specific details.
As shown in figure 1, the breeding method of the rice genic male sterile line with the fine grain odor type and high quality temperature-sensitive rice comprises the following steps:
step 1: guangxi Hengmao agricultural science and technology Limited, hengmao species science research institute in Nanning City, 20 years late rice was bred by Shenzhen research institute from Qinghua university, two-line sterile line deep 08S with good leaf morphology, strong tillering capacity and low transformation critical temperature as female parent, and high-quality maintenance intermediate material B32028[ (92B// Yinfeng B/blue B) F 4 Substitute for Chinese traditional medicine]Performing hybridization improvement, and harvesting about 150 seeds; early rice transplanting formation in 2014F 1 Generation 100, hybrid progeny shows good leaf morphology, big ear, slender grain, strong heterosis, mixed harvest F when mature 1 About 500 grams of kernel substitute; same year late rice transplanting F 2 Separating about 5000 plants in generation, heading at high temperature in autumn, selecting 63 sterile individuals with iodine loss as main part and thorough abortion according to target character performances such as plant leaf morphology, tillering force, ear grain structure, flowering habit, stigma development degree and grain type, combining microscopic pollen abortion performance, cutting stump for regeneration, selfing and fructifying at low temperature in short days in late autumn of Nanning, and harvesting F 2 And (5) seed generation.
Step 2: in 2015, 63F plants were formed in single-plant transplanting of early Oryza sativa 3 And (3) generation strains, preferably selecting 10 plants of 8 strains in the 4-5 stage of young ear differentiation, transferring into a cold water irrigation treatment pool, performing circulating irrigation treatment for 12 days by using 22 ℃ cold water, transferring, selfing and fructifying, and selecting the plants with low harvest fructification rate and grainSeeds of 35 individual plants with long and thin grains; sowing 35 individual seeds of late rice in the same year in Nanning, and transplanting the individual seeds to form F 4 And (3) generating 35 strains, optimally selecting 20 target single plants of 6 strains in the 4-5 period of young ear differentiation, transferring into a cold irrigation pool for cold water treatment and transformation, bagging, selfing and fructifying, and selecting and harvesting 23 single plant seeds with low selfing fructification rate and long and thin grains.
And step 3:2016 early rice planting in Nanning 5 Selecting 40 single plants of 5 target plants in the 4-5 stage of young ear differentiation, performing cold water irrigation treatment and transformation, bagging, selfing and maturing, and preferably selecting 19 long and thin single plants in grain type during maturation to harvest seeds; meanwhile, the strains L61345 and L61347 are preferably selected to carry out the test cross test with a plurality of high-quality restorer lines during the ear emergence of the sterile line. 2016 year old late rice planted in Nanning area F 6 Carrying out cold water irrigation treatment and transformation on half of the selected 4 target plants after roguing of the 4 generation plants, isolating, selfing and fructifying, preferably singly harvesting 17 target single plants during maturation, and mixing the rest plants according to the plant strains; the other half of the plants of the preferable line are subjected to extensive test crossing with a self-selected high-quality restorer line, and a test of test crossing is planted at the same period, and a combined retest test with obvious hybrid vigor is preferable.
And 4, step 4:2017 planting in Nanning F 7 Selecting two strains with the numbers of L71636 and L71638 with stable and consistent agronomic characters in the young ear differentiation period of 4-5, removing impurities and inferior, transferring the inferior parts into a cold irrigation pool to perform cold water irrigation transformation, isolating, selfing and fructifying, selectively harvesting 21 target individual plant seeds when the seeds are mature, and mixing the rest strains; plant and row comparison and yield comparison tests are carried out when a combination contract is measured and matched; preferably, the test cross combination with obvious heterosis is used for small trial seed production. Late rice planting formation in 2017F 8 Carrying out roguing and roguing on 21 generations of strains, preferably, transferring part of the strains with the serial number of L72679 into a cold irrigation pool for cold water irrigation treatment and transformation, isolating, selfing and fructifying, selectively harvesting 25 target individual plant seeds when the seeds are mature, and mixing the rest; in 2017, the late rice is simultaneously combined with seedlings in Guangxi and Jiangxi to carry out multi-point test; and preferably selecting strong and excellent combination trial seed production according to the test performance.
And 5:2018 early rice planted in Nanning form F 9 Generation 25 strainsPartially transferring the strain with the number of L81690 after the young ear is differentiated to 4-5 into a cold irrigation pool for cold water irrigation treatment and transformation, isolating, selfing and maturing, selectively harvesting 17 target individual seeds when mature, and mixing the rest; meanwhile, seedling head combination is arranged to be tested at multiple points in Guangxi and Jiangxi and seed production is tested in spring seasons in Hainan. Late rice planting F in 2018 10 And 17 strains are generated, so that the field expression of the preferably formed 17 sterile strains is realized, no obvious difference exists among the strains, the population in the strains is stable, the agronomic performance is consistent, the comprehensive evaluation and selection number L82555 is used as an entry strain, and the tentative name is as follows: yi Xiang S. Roguing, cold irrigation, transformation, isolation, selfing, and harvesting several seeds of each plant to obtain core seeds of Yixiang S. Meanwhile, seedling head combination is arranged to carry out multi-point test seed production in a south China rice growing area and middle and lower Yangtze river rice growing areas, hainan generation-adding propagation and new combination test seed production.
The Guangxi Hengmao agricultural science and technology Limited company of the Guangxi Hengmao S line, nanning institute of Hengmao science and technology, from Qinghua university Shenzhen institute of institute, selected the two-line sterile line deep 08S (good plant leaf morphology) with good leaf morphology, strong tillering ability and low transformation critical temperature as the female parent, and self-bred two-line sterile line intermediate material (92B// Yinfeng B/blue B) F 4 The generation is used as a male parent for sexual hybridization, and the male parent is obtained by multi-generation directional selection pedigree breeding.
Approximate breed name selected: one of the S parents of Yi Xiang, jiang 08S is Jiang 08S, which has a blood margin of one-half of Jiang 08S. The blood relationship between the two is similar, and the suitable planting areas are the same or similar.
The application variety Yixiang S integrates main excellent genetic components such as good leaf shape, strong tillering force, strong combining ability and the like of a deep 08S plant, has the advantages of keeping the grain shape of the material slender, fragrant and good taste and the like, has obvious difference with the original parent in 7 characters such as the green degree of an inverted two-leaf blade, the number of single plant ears, the longest length of the ears, the thousand kernel weight, the kernel width, the brown rice fragrance and the like, and belongs to different varieties; yi Xiang S has never been sold or authorized for use by anyone else.
Application for product Yi Xiang S compared with similar product deep 08S: the green degree of the leaves with two leaves, the number of ears of a single plant, the longest length of the ears, the thousand grain weight of grains, the width of grains, the shape of grains, the width of brown rice, the shape of brown rice, the fragrance of brown rice and other 9 shapes have obvious differences. Has specificity.
Green degree of the inverted two-leaf blade: the applied variety is medium, the similar variety is deep, the code difference is two, and the applied variety belongs to quality characters and has obvious difference;
the number of ears of each plant is as follows: the applied varieties are many, the similar varieties are medium, the code difference is two, the varieties belong to quantitative characters, and the difference is obvious;
the longest length of the spike: the applied variety is extremely short, the similar variety is short, the code difference is two, belongs to quantitative characters, and has obvious difference;
thousand grain weight of grains: the applied variety is extremely low, the similar variety is low, the code difference is two, belongs to quantitative characters, and has obvious difference;
width of grain: the applied variety is narrow, the similar variety is medium, the code difference is two, and the applied variety belongs to quantitative characters and has obvious difference;
grain shape: the applied variety is slender, the similar variety is oval, the code difference is one, belongs to the quality character, and has obvious difference;
brown rice width: the applied variety is narrow, the similar variety is medium, the code difference is two, the application belongs to quantitative characters, and the application has obvious difference;
brown rice shape: the applied variety is spindle-shaped, the similar variety is half spindle-shaped, the difference of the codes is one, and the applied variety belongs to quality characters and has obvious difference;
brown rice flavor: the applied variety is strong, the similar variety is none, the code difference is two, and the method belongs to quality characters and has obvious difference.
The Yixiang S of the applied variety is planted and observed in the same season for 2018-2019, more than 1000 plants are observed in each crop, the total leaf number of main stems, the growth characteristics, the flowering habit, the morphological characteristics of plants, leaves, ears and grains and the like of the applied variety are consistent, no difference exists among individual plants, no atypical plant is found, and the consistency is realized.
The main economic characters of Yixiang S of the application variety, such as plant height, ear length, total grain per ear, seed setting rate and thousand grain weight, may have certain differences due to different conditions of sowing season, temperature, light, water, fertilizer and the like, and other characters represent the same code in the same growing season and have stability.
Suitable for growing areas or environments, applies for the Yixiang S variety suitable for early and late rice in double cropping rice of south China; planting and cultivating middle rice in one season in middle and lower Yangtze river rice growing areas.
Description of the cultivation techniques
Applying enough base fertilizer and scientifically topdressing: the organic fertilizer is mainly used, nitrogen, phosphorus and potassium are matched, the cultivation is suitable for medium and high fertility horizontal cultivation, the total fertilizing amount per mu is 11-15 kg of pure nitrogen, and N: p: k is as follows 1: 0.5; reasonably close planting, inserting (throwing) 18000-20000 stumps per mu, and properly drying the fields and controlling seedlings when 12 seedlings are planted in each hole; in the later stage, dry and wet management is carried out, and water supply is not cut off too early; comprehensively preventing and treating plant diseases and insect pests.
TABLE 1 Yi Xiang S and deep 08S (control) Properties comparison Table
Comparison of traits shows that Yi Xiang S has 55 traits among 58 shapes in the table, 9 traits: the green degree of the leaves with two leaves, the number of ears of a single plant, the longest length of the ears, the thousand grain weight of grains, the width of grains, the shape of grains, the width of brown rice, the shape of brown rice and the fragrance of brown rice are in different grades with the expression of control characters, and the differences are obvious and have specificity. Other traits were not significantly or not different.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be construed as the protection scope of the present invention.
Claims (8)
1. The breeding method of the fine grain fragrant high-quality thermo-sensitive rice genic male sterile line is characterized in that: the method comprises the following steps:
step 1: selecting a two-line sterile line deep 08S as a female parent, and hybridizing the female parent with a self-bred strain B32028 to obtain seeds;
step 2: carrying out early rice transplanting on the seeds obtained in the step 1 to form F 1 Mixed harvest at maturity F 1 Replacing seeds;
and step 3: late rice transplanting F 2 Generation and harvest F 2 Seed generation:
and 4, step 4: early rice single plant transplanting formation F 3 Generation line and harvest F 3 Seed generation, sowing seeds of individual plants in the late rice of the year, and transplanting the individual plants to form F 4 Generation line, harvest F 4 Seed generation;
and 5: planting early rice in the next year F 5 Generation sterile line, harvest F 5 Generation of seeds, late rice planting in Nanning F 6 Generation line, harvest F 6 Seed generation;
step 6: planting early rice in the next year F 7 Generation line, harvest F 7 Generation seed, planting late rice in the same year to form F 8 Generation line, harvest F 8 Seed generation;
and 7: early rice planting formation F in the next year 9 Generation line, harvest F 9 Generation seed, planting late rice in the same year F 10 Generation line, harvest F 10 The generation seeds are high-quality thermo-sensitive genic male sterile rice.
2. The breeding method of the fine grain odor type high-quality temperature-sensitive rice genic male sterile line according to claim 1, which is characterized in that: the specific process of the step 1 is as follows: two-line sterile line deep 08S with good plant leaf morphology, strong tillering force and low transformation critical temperature is selected as a female parent to be hybridized and improved with a high-quality maintenance intermediate material B32028 with excellent self-bred plant leaf morphology, and 130-150 seeds are harvested.
3. The breeding method of the fine grain flavor type high-quality thermo-sensitive genic male sterile line rice as claimed in claim 1, wherein the breeding method comprises the following steps: the specific process of the step 2 is as follows: the early rice is transplanted to form 100F 1 generation plants, the filial generation shows that the plants have good leaf shape, large ears, slender grains and strong heterosis, and 500 g of F1 generation grains are harvested in a mixed mode during maturation.
4. The breeding method of the fine grain odor type high-quality temperature-sensitive rice genic male sterile line according to claim 1, which is characterized in that: the specific process of the step 3 is as follows: transplanting late rice in the same year into F2 generation, separating 5000 plants of the group, heading in autumn, selecting 63 sterile single plants which are mainly iodine-abortive and have thorough abortive in combination with microscopic pollen abortion performance according to plant leaf morphology, tillering power, ear grain structure, flowering habit, developed degree of stigma and target character performance of grain type, cutting roots for regeneration, selfing and fructifying in short days in late autumn, and harvesting F2 generation seeds.
5. The breeding method of the fine grain flavor type high-quality thermo-sensitive genic male sterile line rice as claimed in claim 1, wherein the breeding method comprises the following steps: the specific process of the step 4 is as follows: early rice single plant is transplanted to form 63F 3 Selecting 10 plants of 8 plants in the stage of young ear differentiation 4-5, transferring into a cold water irrigation treatment pool, performing circulation irrigation treatment for 12 days by using 22 ℃ cold water, transferring, selfing and fructifying, selecting and harvesting 35 single plants with low fructification rate and long and thin grains, sowing 35 single plants of late rice in the same year, and transplanting the single plants to form F 4 And (3) 35 lines of generation, optimally selecting 20 target single plants of 6 lines in the young ear differentiation stage of 4-5, transferring the plants into a cold irrigation pool for cold water treatment, deleting transformation, bagging, selfing and fructifying, and selecting and harvesting 23 single plant seeds with low selfing fructification rate and long and thin grains.
6. The breeding method of the fine grain odor type high-quality thermo-sensitive genic male sterile rice according to claim 1, which is characterized in that: the specific process of the step 5 is as follows: early rice planting in Nanning F 5 Generating 23 sterile lines, selecting 40 single plants of 5 target lines in 4-5 stages of young ear differentiation, performing cold water irrigation, transformation, bagging, selfing, maturing, selecting 19 long and thin single plants of grain type, and harvesting seedsSimultaneously, selecting lines L61345 and L61347 and carrying out test crossing tests with a plurality of high-quality restorer lines when the sterile lines are ear-pulled, and planting the late rice F 6 Selecting 4 target plants from 19 plants, removing impurities and inferior plants, transplanting half of the plants to carry out cold water irrigation treatment and transformation, isolating, selfing and fructification, selecting 17 target single plants to singly harvest when the plants are mature, mixing the rest plants according to the plants, selecting the other half of the plants to carry out wide test cross with a self-selected high-quality restorer line, planting test cross tests in the same period, and preferably selecting a combined retest test with obvious hybrid vigor.
7. The breeding method of the fine grain flavor type high-quality thermo-sensitive genic male sterile line rice as claimed in claim 1, wherein the breeding method comprises the following steps: the specific process of the step 6 is as follows: planting early rice in the next year F 7 Selecting two lines with the serial numbers of L71636 and L71638 and the agronomic characters of which tend to be stable and consistent in young ear differentiation 4-5 period, removing impurities and inferior, transferring the inferior parts into a cold irrigation pool for cold water irrigation transformation, isolating, selfing and fructifying, selectively collecting 21 target single-plant seeds in maturity, mixing the rest according to the lines, carrying out plant row comparison and yield comparison tests in testing and matching group contracts, selecting test cross combinations with obvious hybrid advantages for small test seed production, and forming F test seed production in late rice planting in the year 8 And (3) generating 21 strains, selecting the strains with the number of L72679, removing impurities and inferior, transferring the removed parts into a cold irrigation pool for cold water irrigation treatment and transformation, isolating, selfing and fructifying, selectively harvesting 25 target individual plant seeds during maturity, mixing the rest seeds, simultaneously arranging seedling heads of the late rice in the same year for combination, testing a plurality of points, and selecting strong and excellent combination for trial seed production according to test performance.
8. The breeding method of the fine grain flavor type high-quality thermo-sensitive genic male sterile line rice as claimed in claim 1, wherein the breeding method comprises the following steps: the specific process of the step 7 is as follows: early rice planting formation F in the next year 9 Selecting 25 strains in generation, selecting the strain with the number of L81690 in the stage of young ear differentiation of 4-5, removing inferior parts, partially transferring into a cold irrigation pool for cold water irrigation treatment and transformation, isolating, selfing and fructifying, selectively harvesting 17 target individual seeds when the strain is mature, mixing the rest, arranging the combination of seedling heads at a plurality of tests, and testing and preparing seeds in spring season, wherein F is planted in late rice in the same year 10 Generation 17 plantsThe method comprises the following steps of selecting 17 sterile lines to form field performance, having no obvious difference among the lines, having stable population in the lines and consistent agronomic performance, comprehensively evaluating and selecting a serial number L82555 as a selected line, obtaining the rice of the fine grain odor type high-quality temperature-sensitive genic male sterile line, removing impurities and inferior quality of the selected line, carrying out cold irrigation treatment transformation, carrying out isolated selfing and fructification, after selecting and collecting a plurality of single plant seeds, mixing and collecting the seeds as core seeds of the fine grain odor type high-quality temperature-sensitive genic male sterile line, arranging a plurality of point test tests of seedling head combination in different regions, simultaneously carrying out generative propagation and new combination test seed production.
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| CN116686701A (en) * | 2023-07-24 | 2023-09-05 | 江西兴安种业有限公司 | Method for testing rice dual-purpose genic male sterile line mating force in batches in low generation |
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| Title |
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| 黄春毓等: "籼型香稻温敏核不育系昱香S的选育与应用", 杂交水稻, pages 1 - 4 * |
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| CN116686701A (en) * | 2023-07-24 | 2023-09-05 | 江西兴安种业有限公司 | Method for testing rice dual-purpose genic male sterile line mating force in batches in low generation |
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