CN115372615A

CN115372615A - Detection device

Info

Publication number: CN115372615A
Application number: CN202211308064.9A
Authority: CN
Inventors: 蔡政君; 商涛; 唐林勇
Original assignee: Acon Biotech Hangzhou Co Ltd
Current assignee: Acon Biotech Hangzhou Co Ltd
Priority date: 2022-10-25
Filing date: 2022-10-25
Publication date: 2022-11-22

Abstract

The invention provides a detection device, which comprises a sample collector and a detection box, wherein the sample collector comprises a sampling head, a handle and a connecting part between the sampling head and the handle; the detection box comprises a containing cavity and a buckle, and the connecting part of the sample collector comprises a wedge-shaped structure with a wide upper part and a narrow lower part. After putting into the chamber of accomodating of detecting box with the sampling head of sample collector, the wedge structure of sample collector extrudees the buckle downwards, makes the buckle outwards open, and after the wedge structure passed through the buckle, the buckle kick-backed and hooked the wedge structure. The detection device can be used in the detection fields of disease diagnosis, drunk driving test, drug sucking condition analysis, hormone condition analysis and the like, and can meet the field detection requirement of rapid sampling and rapid detection. The portable detector has compact and small structure, is very convenient to carry, and belongs to a portable detection device.

Description

Detection device

Technical Field

The invention relates to the technical field of rapid medical detection, in particular to a portable detection device.

Background

The carrier of a conventional disposable rapid detection product is basically in the form of a detection test paper or a detection kit, for example, a detection test paper of a detection product adopting a Lateral flow (lareal flow) mode generally includes a bottom card, on which a sample pad, a marker binding pad (also called a marker pad for short, usually glass fiber is used as a carrier), a test pad (usually an NC membrane is used as a carrier), and a water absorption pad (usually a filter paper and other water absorption materials) are sequentially and mutually overlapped from upstream to downstream, and the transmission of a sample on the test paper and the detection result are realized by using an immunochromatography principle. The label pad includes a label capable of binding to an analyte, such as latex, colloidal gold, fluorescent microspheres, etc. labeled with an antigen or an antibody. The test pad is generally provided with a detection line and a quality control line. As the sample flows over the strip, the label may be captured and accumulate or not captured on the detection line. The presence or concentration of the analyte is determined based on a signal from the label, such as a color signal or a fluorescent signal. The quality control line can be used for judging whether the test paper is effective or for positioning when an instrument reads a detection result and the like. The detection box is characterized in that the detection test paper is placed between the upper cover and the lower plate, the upper cover is provided with a sample adding hole corresponding to a sample pad of the detection test paper, and the upper cover is provided with an observation window corresponding to a test pad of the detection test paper.

Disposable rapid detection and diagnosis products for detecting diseases or other physical conditions by using body fluids such as urine, blood or other body fluids of human bodies are widely used all over the world, and the application places of the disposable rapid detection and diagnosis products can be laboratories operated by professional personnel, and also can be operated by non-professional personnel at home, schools, markets, road gates, customs and other places.

Such conventional detection products are only simple test strips or test cartridges, and when sampling and detecting are performed by using a sampling rod, the sampling rod needs to be placed in a collection bottle after sampling is completed, and a sample on the sampling rod is extruded to the collection bottle and then dropped into a sample pad or a sample adding hole, as shown in fig. 7 and 8 of US patent application US20040237674A1, such sample adding operation is complicated. The Chinese patent CN200420110153.3 and the Chinese patent CN201010164579.7 respectively improve the detection box, and simplify the sample adding operation steps. The sample adding hole of the Chinese patent CN200420110153.3 detection box protrudes out of the upper cover of the detection box to form a receiving cavity, the inner space of the receiving cavity can contain the water absorbing material on the sampling rod, and the water absorbing material is placed in the receiving cavity, and the liquid sample is extruded to the sample pad through extrusion. The sample adding hole of the Chinese patent CN201010164579.7 is provided with a channel, the channel allows a sampling head to pass through, a liquid sample is extruded by extrusion, and the sample is transferred to a sample pad through a drainage piece. These two chinese patents have some problems, such as: a user needs to press the sampling rod all the time to keep the extrusion state until the liquid sample is completely extruded, and if the sampling rod is loosened in the midway, the liquid sample remained on the sampling rod after the extrusion force of the sampling rod is lost cannot reach the sample pad, so that the risk of insufficient sample adding amount is increased; the sample rod may fall out of the sample adding hole or the sample adding channel after being loosened to pollute the surrounding environment; the sample adding hole or the sample adding channel arranged on the upper cover is blocked by the sampling rod when in sample adding, the liquid sample can not be conveyed to the test paper below in time and is conveyed away, but is accumulated in the sample adding area, and the extruded liquid sample can be extruded by the sampling rod and overflows or splashes out of the sample adding area, even splashes to the body of an operator, and causes danger to the operator. In addition, the existing sampling rod needs a large applied force when the sampling component is extruded, the stroke of the sampling rod is also large, large impact is easily caused to the extruded liquid sample, and the extruded liquid sample may be extruded by the sampling rod to overflow or splash out of a sample adding area, even splash onto the body of an operator, so that danger is caused to the operator.

Therefore, how to provide a detection device to realize rapid detection and avoid the sample from splashing out of the sample application region in the sample application process is a technical problem that needs to be solved urgently by those skilled in the art at present.

Disclosure of Invention

The invention provides a detection device, which comprises a sample collector and a detection box and aims to solve the technical problems that a liquid sample collected by the sample collector is not easy to be fully extruded out and the liquid sample is prevented from splashing in the extrusion process.

In order to achieve the purpose, the invention provides the following technical scheme.

The invention provides a detection device, which comprises a sample collector and a detection box, wherein the sample collector comprises a sampling head, a handle and a connecting part between the sampling head and the handle, the detection box comprises a containing cavity and a buckle, the connecting part of the sample collector comprises a wedge-shaped structure with a wide top and a narrow bottom, the buckle of the detection box comprises a pair of inverted hooks, hook tips of the pair of hooks face inwards, the hook tips of the pair of inverted hooks are opposite to each other, after the sampling head of the sample collector is placed into the containing cavity of the detection box, the wedge-shaped structure of the sample collector downwards extrudes the buckle to enable the pair of inverted hooks to be outwards opened, and after the wedge-shaped structure passes through the hook tips, the hooks rebound and hook the wedge-shaped structure.

Further, the wedge structure comprises at least one inclined surface or cambered surface, and when the wedge structure downwards presses the buckle, the inclined surface or the cambered surface guides the clamping hook to be outwards opened.

Further, at least the lower shape of the wedge-shaped structure cross section comprises: inverted trapezoids, inverted triangles, combinations of inverted trapezoids and rectangles, semi-circles or approximate semi-circles.

Furthermore, the cross section of the wedge-shaped structure is inverted in a convex shape.

Furthermore, the width of the bottom of the wedge-shaped structure is smaller than the distance between the two hook tips of the hook.

Further, the width of the bottom of the wedge-shaped structure is less than half of the width of the top of the wedge-shaped structure.

Further, the detection box comprises a second buckle, and the second buckle clamps the sampling head of the sample collector after the sampling head of the sample collector is placed into the containing cavity of the detection device.

Further, the second buckle is arranged in a containing cavity of the detection device.

Furthermore, the tail end of the second buckle is provided with a convex block, the sampling head of the sample collector is provided with a concave part, and after the sampling head of the sample collector is placed into the containing cavity of the detection box, the convex block of the second buckle is buckled in the concave part of the sampling head of the sample collector.

Advantageous effects

The detection device is provided with a wedge-shaped structure which is wide at the top and narrow at the bottom at the connection part of the sample collector, and preferably, the wedge-shaped structure also comprises a pair of inclined surfaces or cambered surfaces with guiding function, so that the wedge-shaped structure can more easily pass through the pair of clamping hooks on the detection box, and the pressing force applied to the sample collector is reduced. In addition, the bottom size of the wedge-shaped structure is smaller than the distance between the hook tips of a pair of hooks matched with the wedge-shaped structure on the detection box, so that the bottom of the wedge-shaped structure enters the lower part of the hook tips of the pair of hooks under the condition that the bottom of the wedge-shaped structure is not blocked, the sliding distance of the wedge-shaped structure along the hooks on the detection box is shortened in the extrusion process, the impact of the sample collector on the extruded liquid sample is effectively reduced, and the splashing of the liquid sample is effectively reduced. Therefore, the liquid sample splashing detection device can effectively reduce the liquid sample splashing out of the detection device in the detection process, prevent the liquid sample from polluting the detection surrounding environment, prevent the liquid sample from splashing on the body of an operator, and reduce the risk of harming the body of the operator. And, detection device passes through pothook and wedge structure block, makes the sample collector kept on detecting the box, and the sample pad can be fully extruded, and the sample is fully extruded, has avoided the not enough problem that leads to detecting the failure of application of sample volume to and avoided the sampling rod to fall out and pollute the surrounding environment from application of sample hole or application of sample passageway.

Drawings

FIG. 1 is a schematic view showing an initial use state of the detecting unit of the present invention, i.e., a state when a sample collector is just inserted into a cartridge.

FIG. 2 is a schematic view of the sample collector of the detecting unit of the present invention when it is fully inserted into the cartridge.

FIG. 3 is a perspective exploded view of the detecting device of the present invention.

FIG. 4 is a schematic view of a lateral flow format test strip.

Fig. 5 is a schematic perspective view of a sample collector.

FIG. 6 is a perspective view of the assembled cartridge of the present invention.

FIG. 7 is a top view of the cartridge of FIG. 6.

Fig. 8 isbase:Sub>A sectional view taken alongbase:Sub>A-base:Sub>A direction of fig. 7.

Fig. 9 is a cross-sectional view taken along the line B-B in fig. 7.

Fig. 10 is a top view of fig. 2.

FIG. 11 is an enlarged partial schematic view of the area within circle "A" of FIG. 5.

FIG. 12 is a schematic cross-sectional view of the beginning of the insertion of the sample collector into the test cassette.

FIG. 13 is a schematic cross-sectional view showing a process of inserting a sample collector into a cartridge.

FIG. 14 is a schematic cross-sectional view of the sample collector fully inserted into the test cassette.

Fig. 15 is a partially enlarged schematic view within circle "B" in fig. 14.

Fig. 16 is a sectional view taken along the direction C-C of fig. 11.

Fig. 17 to 23 are schematic views of modified designs of fig. 16.

Fig. 24-26 are illustrations of the design shown in fig. 23, showing the process of inserting the connection into the buckle.

Fig. 27-29 are illustrations of the design shown in fig. 16, showing the process of inserting the connection into the clasp.

Fig. 30 is a partially enlarged schematic view within circle "C" in fig. 24.

Fig. 31 is a partially enlarged schematic view within circle "D" in fig. 27.

Fig. 32 is a comparative schematic diagram of the two schemes shown in fig. 24 and 27 overlaid together (the dashed line is the scheme of fig. 24, and the solid line is the scheme of fig. 27).

Detailed Description

The present invention will be described in detail below with reference to the accompanying drawings. These specific examples are given by way of illustration only and are not intended to exclude other specific embodiments that would be produced by one of ordinary skill in the art in conjunction with the present invention without departing from the spirit thereof.

Defining: the "lower part" in this patent specification includes a "bottom" of a certain component and a part extending upward from the "bottom" of the component by an appropriate distance.

The test device shown in fig. 1 to 9 includes a test cartridge 300 and a sample collector 200 used in cooperation with the test cartridge. The test cassette includes an upper cover 1, a lower plate 2, and a test paper 100, and the test paper 100 is located between the upper cover and the lower plate of the test cassette. In one embodiment, the upper cover 1 and the lower plate 2 are fastened to each other and the test strip 100 is mounted in the test cassette 300.

As shown in fig. 4, the test strip 100 is a lateral flow type test strip, and includes a bottom card 101 on which a sample pad 102, a label-binding pad 103, a test pad 104, and an absorbent pad 105 are adhered in order from upstream to downstream (i.e., a liquid sample transport direction) on the bottom card 101. The test strip 100 may also be a vertical flow format test strip or other format test strip known to those skilled in the art.

The sample collector 200 shown in fig. 5 comprises a handle 201, a connection 202 and a sampling head 203, the connection 202 being interposed between the handle 201 and the sampling head 203. The sampling head 203 comprises a sampling pad 204, and the sampling pad 204 is fixed on the sampling head 203 by means of snap-fit or adhesion. The sampling pad 204 may be made of absorbent tampon, sponge, absorbent fiber or porous polymer material, or other absorbent materials known in the art, such as polyvinyl alcohol. The sampling head 203 can also be provided with a transparent indicating area 205, the indicating area is positioned above the sampling pad 204, and an indicating test paper is stored in the indicating area 205, when the sampling pad 204 is saturated with the liquid sample, the liquid sample can contact the indicating test paper, so that the indicating test paper is changed from a first color to a second color, and an operator can judge whether the sample is sufficiently collected according to the color change condition of the indicating test paper. The handle 201 may be designed to have a flat configuration with a certain curvature to facilitate grasping by an operator, and the connecting portion 202 is designed to have a narrow neck shape.

As shown in fig. 6, the upper cover 1 of the cartridge 300 is provided with a sample inlet 3 and an observation window 4, the sample inlet 3 is positioned above the sample pad 102 of the test strip, and the observation window 4 is positioned above the test pad 104 of the test strip. And a surrounding barrier 5 is arranged at the surrounding area of the sampling hole 3. The enclosure 5 can enclose the sampling hole 3 in the upper cover in a manner of standing on the upper surface of the upper cover and form a containing cavity 6; the enclosure 5 may also enclose the sample inlet 3 therein in a manner of sinking downward from the upper surface of the upper cover and extending into the test cassette to form a receiving chamber 6. The enclosure 5 may have a part standing on the upper surface of the upper cover 1, and a part extending toward the inside of the detection box to enclose the sample inlet 3 therein and form a receiving chamber 6. The enclosure 5 is a generally enclosing wall structure having a receiving cavity 6 therein. The enclosure 5 includes a front enclosure 51, a rear enclosure 52 and two side enclosures 53. When the sampling head 203 of the sample collector 200 is inserted into the receiving cavity 6, the front wall 51 is located in the top direction of the sample collector, and the rear wall 52 is located in the connecting portion 202 direction of the sample collector. The area of the receiving cavity 6 near the front rail 51 may be referred to as a front end of the receiving cavity, and the area of the receiving cavity near the rear rail 52 may be referred to as a rear end of the receiving cavity 6.

The bottom of the containing cavity 6 comprises a bottom plate 7, and the sampling hole 3 is arranged on the bottom plate 7. After the sampling head 203 is completely inserted into the receiving cavity 6, the bottom of the sampling pad 204 abuts against the bottom plate 7, the sampling head 203 presses the sampling pad 204 downwards, so as to press the liquid sample out of the sampling pad 204, the pressed liquid sample enters the sample pad 102 of the test paper through the sample inlet 3, and then the test is started. In one embodiment, the bottom plate 7 may be a part of the top cover plate, and the enclosure 5 is formed by a circle formed by the top cover plate protruding upward at a proper height, and the cavity in the circle forms the receiving cavity 6. The bottom plate 7 can be provided with a plurality of sample holes 3 above the test paper sample pad 102, for example, two sample holes are arranged at intervals, so that on one hand, an extruded sample can be quickly introduced into the test paper sample pad 102, and on the other hand, the strength of the bottom plate 7 is maintained, so that the bottom plate is not easy to deform in the extrusion process.

In one embodiment, three channels 8 are provided at the junction of the bottom plate 7 and the back wall 52, and the channels 8 penetrate through the bottom plate 7, so that the channels 8 communicate with the inside of the test box, so that the squeezed liquid sample can be introduced into the test box or onto the sample pad 102 of the test paper and absorbed by the sample pad 102 of the test paper.

In another embodiment, an exhaust hole 16 is formed at the lower part of the rear wall 52 of the receiving cavity 6, the exhaust hole 16 penetrates through the bottom plate 7, and the exhaust hole 16 is communicated with the inside of the detection box, so that the liquid sample flowing to the rear wall 52 can enter the inside of the detection box 300 through the exhaust hole 16.

In yet another embodiment, channels 8 in the base plate correspond to vents 16 in the lower edge of rear barrier 52, and channels 8 extend to vents 16 in the lower portion of the rear barrier. More specifically, the flow guide groove 8 on the bottom plate is connected with the drain hole 16 on the rear fence to form a right-angle opening or an "L" opening. Such design can further increase the flux that liquid sample flows into in the test kit, avoids having more liquid sample to be detained in collecting cavity 6 in the in-process of extrusion sample collector, leads to liquid sample splash receiving cavity 6.

In still another embodiment, the rear end of the receiving cavity 6 of the detection box is provided with a diversion trench 8 on the bottom plate 7, an effusion hole 16 is provided on the lower part of the rear enclosure 52, a lateral effusion hole 24 is provided on the lower part of the side enclosure 53, the diversion trench 8 extends towards the effusion hole 16 and the lateral effusion hole 24 respectively and intersects with the effusion hole 16 and the lateral effusion hole 24, and the effusion hole 16 is connected with the lateral effusion hole 24, so that two corners of the rear part of the receiving cavity 6 are formed with an opening jointly formed by the diversion trench 8, the effusion hole 16 and the lateral effusion hole 24 to divert the liquid sample gathered at the rear part of the receiving cavity 6.

Fig. 12, 13, and 14 illustrate a process of inserting the sample collector 200 into the cartridge 300. FIG. 15 illustrates a schematic distribution of the expressed liquid sample 500 (shown as densely-distributed dots in FIG. 15) after the sample collector 200 is fully inserted into the test cartridge 300.

The size and height of the opening of the drain hole 16, the distance between the drain hole 16 and the bottom plate 7 (for example, the drain hole may penetrate the bottom plate, or may not penetrate the bottom plate), the number of the drain holes, and the like can be adjusted according to the sampling amount of the sample collector, the capacity of the receiving cavity, the sampling speed of the sampling hole, and the like.

As shown in fig. 1 and 2, the upper cover 1 is further provided with a fastening device of the sample collector. Preferably, the fastening device is integrally formed with the upper cover 1 from plastic. The fastening device comprises a clasp 10. Preferably, the catch 10 is formed on the top plate of the upper cover 1 and is located near or beside the receiving cavity 6; more preferably, the clasp 10 is located adjacent or alongside the back rail 52. The position of the buckle 10 may also be in other suitable positions as long as it can achieve the function of the present invention, and therefore, the position of the buckle 10 should not limit the scope of the claimed technical solution. In a preferred solution, the buckle 10 comprises a pair of inverted hooks 14 (the hooks 14 should have suitable elasticity) extending upwards from the top plate of the upper cover 1 by a suitable height, the tips 15 (as shown in fig. 30) of the pair of hooks facing each other inwards. Correspondingly, the connecting portion 202 of the sample collector 200 includes a wedge-shaped structure 202a (shown in fig. 5 and 11) with a wide top and a narrow bottom. Referring to fig. 12-14 and 27-29, after the sampling head 203 of the sample collector is placed in the receiving cavity 6 of the test cassette, the lower portion of the wedge-shaped structure 202a of the sample collector corresponds to the latch 10. When a proper amount of downward pressure is applied to the handle, the wedge-shaped structure 202a presses the buckle 10 downward, and the wedge-shaped structure 202a or the inclined surface 202b or the arc surface 202c of the wedge-shaped structure 202a props up the upper parts of the pair of hooks 14, even if the upper parts of the pair of inverted hooks 14 generate proper elastic deformation, the upper parts of the hooks 14 expand outward, and further the gap between the pair of hook tips 15 is enlarged, so that the wedge-shaped structure 202a passes through the pair of hook tips 15, and then the pair of hooks 14 rebound, the pair of hook tips 15 hook the wedge-shaped structure 202a, the sampling head 203 is kept in the receiving cavity 6, and the liquid sample in the sampling pad 204 is continuously pressed out. Preferably, the top of the pair of inverted hooks 14 is provided with a slope 15a (as shown in fig. 30) for guiding the wedge-shaped structure 202a to press the hook tip 15, so as to further reduce the resistance of the wedge-shaped structure 202a to pass through the pair of hook tips 15, i.e. reduce the force applied to the handle 201 of the sample collector, thereby reducing the impact on the squeezed liquid sample and preventing the liquid sample from splashing out of the receiving cavity 6.

As shown in fig. 16-18 and 20, the wedge-shaped structure 202a includes at least one inclined surface 202b (preferably including a pair of inclined surfaces 202 b), or as shown in fig. 21 and 22, the wedge-shaped structure 202a includes at least one curved surface 202c (preferably including a pair of curved surfaces 202 c), and when the wedge-shaped structure 202a presses the hook 14 downward, the inclined surface 202b or the curved surface 202c guides the upper portion of the hook 14 to expand outward, i.e., the inclined surface 202b or the curved surface 202c presses the pair of hook tips 15 or the inclined surfaces 15a of the hook tips outward, pushing the upper portion of the hook 14 to expand outward, making the gap between the pair of hook tips 15 large, so that the wedge-shaped structure 202a passes through the pair of hook tips 15 more easily, and then the upper portion of the hook 14 resiliently rebounds to make the pair of hook tips 15 hook the top of the wedge-shaped structure 202a hook.

Fig. 16-22 illustrate several specific configurations of the wedge-shaped structure 202a, respectively. In general, the wedge-shaped structure 202a has a cross section that is generally wider at the top and narrower at the bottom, thereby facilitating insertion of the wedge-shaped structure into the hook 14. Specifically, in some aspects, the shape of the entire cross-section of the wedge-shaped structure 202a or the lower shape of the cross-section of the wedge-shaped structure includes: an inverted trapezoid or isosceles trapezoid (as shown in fig. 16), an inverted triangle or isosceles triangle (as shown in fig. 17 and 18), a combination of an inverted trapezoid and rectangle (as shown in fig. 20), a semi-circle (as shown in fig. 21), or an approximate semi-circle (as shown in fig. 22). In the embodiment shown in FIG. 19, the wedge-shaped structure 202a has an inverted "dogbone" shape in cross-section. In the embodiment shown in fig. 20, the cross-section of the wedge-shaped structure 202a may also be considered as an approximately inverted "convex" shape. In the embodiment shown in fig. 23, the connecting portion 202 does not include a wedge-shaped structure, and has a rectangular cross-section including a square and a rectangle.

Fig. 24-26 demonstrate the process of inserting the connecting portion 202 into the pair of hooks 14 when the connecting portion 202 does not include a wedge-shaped structure, i.e., it is rectangular in cross-section (as in the embodiment shown in fig. 23). Fig. 27-29 demonstrate the process of inserting the wedge-shaped structure 202a into the pair of hooks 14 when the connecting portion 202 includes the wedge-shaped structure 202 a. Comparing the two, it is clear that the solution in which the connection portion comprises the wedge-shaped structure 202a is superior to the solution in which the connection portion does not comprise the wedge-shaped structure. As will be further discussed in detail below.

As shown in fig. 30, when the cross section of the connection portion 202 is rectangular (i.e., the connection portion 202 does not include a wedge structure, and the upper and lower portions thereof have the same size), the width dimension d3 of the cross section thereof must be larger than the distance d2 between the pair of hooking tips 15, otherwise, the pair of hooking tips 15 cannot hook the connection portion 202 when the connection portion 202 enters the lower side of the pair of hooking tips 15. As shown in fig. 31, when the connection portion 202 includes the wedge-shaped structure 202a, the top dimension d3 of the wedge-shaped structure 202a is larger than the distance d2 between the pair of hook tips 15, and the bottom dimension d1 of the wedge-shaped structure 202a is smaller than the distance d2 between the pair of hook tips 15. Taking the specific structure of the wedge-shaped structure 202a shown in fig. 16 as an example, when the wedge-shaped structure 202a is pressed downward through the pair of hook tips 15, the bottom 202d of the wedge-shaped structure 202a first passes through the pair of hook tips 15 in a non-pressed state until the pair of inclined surfaces 202b of the wedge-shaped structure 202a (or the arc surfaces 202c shown in fig. 21 and 22) press the pair of hook tips 15 or the inclined surfaces 15a of the pair of hook tips. Referring to fig. 32, the wedge-shaped structure is designed to be lowered by a greater height "h" when the wedge-shaped structure 202a is in a non-compressed state, compared to the design of the connecting portion 202 having a rectangular cross-section, i.e., the wedge-shaped structure 202a is lowered by a shorter distance "h" during compression through the pair of hook tips 15 than the design of the connecting portion 202 having a rectangular cross-section. In addition, the pair of inclined surfaces 202b or the cambered surfaces 202c of the wedge-shaped structure 202a has a guiding function in the process, so that the pressing force required to be applied to the handle 201 of the sample collector is greatly reduced, the wedge-shaped structure 202a of the sample collector can more easily enter the buckle 10, and when the sampling head 203 extrudes the liquid sample collected by the sampling pad 204, the impact on the extruded liquid sample is reduced, so that the splashing of the liquid sample is reduced, and the defects in the prior art and the technical scheme shown in fig. 23 are well overcome.

As shown in FIG. 31, in a preferred embodiment, the width d1 of the wedge-shaped structure bottom 202d is less than one-half the width d3 of its top 202 e. The relationship between the width d1 of the base 202d of the wedge-shaped structure, the spacing d2 between the pair of hook tips 15 when the hook is not compressed, and the width d3 of the top 202e of the wedge-shaped structure is: d1 is less than d2 and d2 is less than d3.

The design of the connection portion 202 of the sample collector to include the wedge-shaped structure 202a is advantageous in that the connection portion 202 can be more easily pressed and pass through the pair of hook tips 15, and the size of the connection portion 202 in the vertical direction can be ensured to be large enough, so that the connection portion 202 has enough strength and rigidity, and thus the connection portion 202 is not easily deformed or broken during the pressing process. The pair of hooks 14 engage with the wedge-shaped structures 202a, so that the sample collector 200 is held on the test cassette 300, the sample pad 204 is sufficiently pressed, and the sample is sufficiently pressed out.

Referring to fig. 1, 2, 6-8 and 12-14, the testing cassette 300 further includes a second latch 9, and after the sampling head 203 of the sample collector is placed in the receiving cavity 6 of the testing device, the second latch 9 latches the front end of the sampling head 203 of the sample collector. Preferably, the

second fasteners

9 and 10 are respectively located at two ends or at the periphery of two ends of the sampling head 203 of the sample collector, so that the design further facilitates applying a proper pressure to the sampling head 203, and further facilitates rapidly extruding the liquid sample collected by the sampling head 203 without generating liquid splash. As shown in fig. 12-14, during the pressing process, the second latch 9 hooks the front end of the sampling head 203, and the sample collector 200 performs a lever movement with the contact portion of the second latch 9 and the sampling head 203 as a fulcrum until the wedge-shaped structure 202a of the connection portion 202 of the sample collector presses and passes through the pair of hook tips 15 and is held under the pair of hook tips 15. The liquid sample within the sampling head 203 is sufficiently squeezed out (as shown in fig. 15).

Preferably, the second catch 9 is arranged inside the housing chamber 6 of the detection device.

In order to further improve the fastening effect of the second fastener 9, the end of the second fastener 9 is provided with a projection 91 (as shown in fig. 8), the sampling head 203 of the sample collector is provided with a recess 206 (as shown in fig. 1), and after the sampling head 203 of the sample collector is placed in the receiving cavity 6 of the detection box, the projection 91 of the second fastener is fastened in the recess 206 of the sampling head of the sample collector, so as to hook the top end of the sampling head 203.

As shown in fig. 6, the clip 10 is disposed behind the rear rail 52. The gap between a pair of hooks 14 of the buckle 10 is a clamping groove 13, and the clamping groove 13 and the rear surrounding baffle groove 12 are on the same axis. When the sample collector 200 presses the sampling pad 204 on the sampling head completely on the bottom plate 7, the connection part 202 of the sample collector is clamped into the buckle 10 and limited in the clamping groove 13, and after the operator releases the handle 201 of the sample collector, the sampling pad 204 is still kept in a pressed state. The pair of hooks 14 is engaged with the wedge-shaped structure 202a, so that the wedge-shaped structure 202a is confined in the slot 13, the sample collector 200 is held on the detection box 300, the sampling pad 204 is fully pressed, and the sample is fully pressed.

Referring to fig. 1 and 2, the sampling head 203 of the sample collector placed in the receiving cavity 6 presses down the sampling pad 204 with the second buckle 9 as a fulcrum, so that the sampling pad 204 is pressed by the bottom plate 7 to squeeze out the liquid sample in the sampling pad 204. In the example shown in fig. 1 and 2, and fig. 5 to 9, the upper end of the second latch 9 is provided with a projection 91 (as shown in fig. 8), the top end of the sample collector is provided with a recess 206 (as shown in fig. 5), when the sampling head 203 of the sample collector is inserted into the receiving cavity 6, the top end of the sampling head 203 is placed under the second latch 9, the projection 91 of the second latch 9 is latched in the recess 206 of the sample collector (as shown in fig. 1), and then the sample collector handle 201 is rotated with the latched position as a fulcrum to press down the sampling pad 204 to be pressed by the bottom plate 7. The manner in which the fulcrum engagement between the second catch 9 and the sampling head 203 is formed may also be other, such as, but not limited to, the manner in which the upper end of the second catch 9 is provided as a recess and the top end of the sampling head includes a projection that engages with the recess. Or, an opening is formed on the second buckle 9, and a protrusion is arranged at the top end of the sampling head 203 and can be inserted into the opening to form a downward pressing fulcrum. These modifications are not shown.

The second catch 9 preferably has a certain elasticity. The second catch 9 is provided in the receiving chamber 6 at a position close to the front end of the receiving chamber. For example, the second catch 9 may be arranged on the inner wall of the front rail 51. For another example, in the embodiment shown in fig. 7, the second buckle 9 is disposed on the floor region between the sample hole 3 and the cowl 51, and a certain spacing space 11 (shown in fig. 7) is left between the second buckle 9 and the cowl 51. This space 11 leaves a space for the second catch 9 to retract. Specifically, when the sampling head 203 is inserted into the receiving cavity 6, the front end of the sampling head abuts against the latch arm 92 of the second buckle 9 (as shown in fig. 8), the latch arm 92 is pressed and then elastically tilts into the space 11 until the protrusion 91 of the second buckle is latched into the recess 206 of the sampling head, and the latch arm 92 elastically restores to the original position.

In the embodiment in which the spacing space 11 is provided between the second buckle 9 and the cowl 51, the spacing space 11 may also play a role of buffering. In the scheme that the second buckle 9 is arranged on the inner wall of the front wall, the top end of the sampling head 203 inserted into the accommodating cavity 6 is very close to the front wall 51, if the amount of the liquid sample extruded from the sampling pad 204 is larger than the gap between the top end of the sampling head 203 and the front wall 51, and the liquid sample cannot flow into the detection box, the liquid sample in the gap between the top end of the sampling head 203 and the front wall 51 may be extruded out of the front wall 5, so that the space 11 is enlarged to be a buffer area.

Also shown in figures 1, 2 and 6 is a recess 12 in the back rail 52 for receiving a connector 202 of a sample collector. When the sample collector 200 is pressed downwards with the second buckle 9 as a fulcrum, the connecting portion 202 of the sample collector can be inserted into the groove 12 of the back fence, so that the sampling pad 204 of the sample collector 200 can be completely pressed on the bottom plate 7, and each position of the sampling pad 204 can be sufficiently pressed.

EXAMPLE 1 preparation of a Portable novel coronavirus detection device

For example, a portable novel coronavirus (SARS-CoV-2) detection device is prepared.

As shown in fig. 4, a novel coronavirus (SARS-CoV-2) test strip 100 includes a base card 101 on which a sample pad 102, a marker binding pad 103, a test pad 104, and a water absorption pad 105 are adhered, which are sequentially stacked one on another from upstream to downstream, on the base card 101. Wherein the marker binding pad 103 is coated with anti-novel coronavirus (anti-SARS-CoV-2) antibody-latex marker, the detection line (T line) of the test pad is coated with anti-SARS-CoV-2 antibody, and the quality control line (C line) is coated with goat anti-mouse IgG.

The prepared test paper for detecting the novel coronavirus (SARS-CoV-2) is put into the test box 300, so that the sample inlet 3 of the upper cover 1 of the test box is positioned above the sample pad 102 of the test paper, and the observation window 4 is positioned above the test pad 104 of the test paper.

The sample collector 200 is packaged in a sealed bag, and forms a complete detection device with the prepared detection box. The sampling pad 204 of the sample collector 200 will absorb water and swell when contacted by saliva.

EXAMPLE 2 use of the detection device

A portable novel coronavirus (SARS-CoV-2) detection device is used as an example.

The sample collector 200 is removed from the sealed bag and placed in the mouth of the test subject to aspirate saliva to a prescribed sample volume. If the indicating test paper exists, whether the sufficient quantity is collected can be judged according to the color change condition of the indicating test paper. If the indicating test paper is not available, whether the sampling pad has collected enough quantity can be judged according to the expansion degree of the sampling pad.

The sampling head 203 which has collected enough quantity is inserted into the containing cavity 6 of the detection box, the second buckle 9 in the containing cavity is buckled with the concave part 206 at the front end of the sampling head, the sample collector 200 is pressed downwards by taking the second buckle 9 as a fulcrum, the connecting part 202 of the collector is embedded into the groove 12 of the rear enclosure, after the sampling pad 204 of the sampling head is completely pressed on the bottom plate 7 flatly, the connecting part 202 is clamped into the buckle 10 and limited in the clamping groove 13, at the moment, an operator can release the collector, and the sampling pad 204 can be always kept in a pressed state.

The liquid sample that is expressed from the collector sample pad passes through the inlet hole 3 to the sample pad 102 of the test strip. The liquid sample then passes through sample pad 102, label conjugate pad 103, test pad 104 to absorbent pad 105. If the test pad only displays the color at the position of the C line, the sample is negative, if the positions of the C line and the T line both display the color, the sample is positive, further nucleic acid analysis is needed, and if the C line does not display the color, the detection is invalid.

EXAMPLE 3 Portable drug detection device and use

Drug test strip 100 is also known as a drug abuse test strip, "drug abuse" (DOA) is a drug used for non-medical purposes (usually for hallucinogenic effects). Abuse of such drugs can lead to physical and mental harm and, in some cases, dependence, addiction, and even death. Examples of DOAs include cocaine, amphetamines (e.g., black beauties, white bennies, amphetamine tablets, dextroamphetamine drugs, dexes, beans), methamphetamines (crank, methamphetamine, crystal, speed), barbiturates (diazepam, valium, roche Pharmaceuticals, nutley, new Jersey), sedatives (i.e., hypnotics), lysergic acid diethylamide (LSD), sedatives (downs, gooflabs, barbbas, blue devils, yelljarks, lues), tricyclic antidepressants (TCAs, e.g., imipramine, amitriptyline, and doxepin), phencyclidine (PCP), tetrahydrocannabinol (THC, pot, dope, hash, ed, etc.), and tablets (e.g., morphine, opium, herotherapy, heroodene, etc.).

This embodiment takes a portable amphetamine (urine) colloidal gold method detection device as an example.

The portable amphetamine (urine) colloidal gold method detection device comprises detection test paper, a detection box and a sample collector. The detection test paper is portable amphetamine (urine) colloidal gold detection test paper and comprises a bottom card, wherein a sample pad, a marker combination pad, a test pad and a water absorption pad are adhered to the bottom card and are sequentially and mutually overlapped from upstream to downstream. The principle of competing the binding of amphetamine conjugates and possibly amphetamine contained in the urine by monoclonal antibodies. The label conjugate pad contained anti-amphetamine monoclonal antibody labeled with colloidal gold (colloid Jin Kangti) and the test pad T line contained amphetamine conjugate.

In the test, the sample collector enters the urine of the tester, then the urine sample on the collector is pressed into the test box, and the urine sample flows upwards on the test paper under the capillary effect. For example, if the concentration of amphetamine in the urine sample is less than 1000ng/ml, the colloidal gold antibody will not fully bind amphetamine. Thus, colloid Jin Kangti is bound by the amphetamine conjugate immobilized on the test pad during chromatography, and a reddish-purple band appears on the T-line. If the concentration of amphetamine in the urine samples is higher than 1000ng/ml, the colloidal gold antibody binds to all amphetamines, so that no purple-red band appears in the T-line region due to no binding of amphetamine conjugate for a competitive reaction. A negative urine sample will show a purple-red band on the T line due to lack of antibody-antigen competition reaction during the detection process. A purple-red band appeared at line C, regardless of whether amphetamine was present in the urine sample. The mauve band appearing in the quality control zone (C) is a standard for judging whether sufficient urine sample exists or not and whether the chromatography process is normal or not, and is also used as an internal control standard of the reagent.

In the embodiment of using one detection device for detecting multiple drugs of abuse, a plurality of test paper storage grooves are arranged on the lower plate of the detection box and used for storing the test paper of different detection items.

Example 4 Portable immunofluorescence assay (FIA) detection device and use

The test paper of this embodiment is exemplified by time-resolved immunofluorescence assay based on phosphorescence.

The detection test paper is used for detecting IgG antibodies generated after pathogen microorganism infection, and the detection mode is an indirect method mode. The preparation method of the test paper comprises the following steps: labeling anti-human immunoglobulin IgG antibody with phosphorescent material, and fixing the phosphorescent material on the label conjugate pad; antigens of pathogenic microorganisms to be analyzed are immobilized on the detection line of the test pad, and IgG is immobilized on the quality control line of the test pad.

During detection, a liquid sample on the collector is extruded into the detection box, then the collector is pulled out, the detection box provided with the detection test paper is inserted into a fluorescence analyzer, and the detection result is analyzed through the phosphorescent signal intensity values of the T line and the C line of the detection test paper. When the detection line and the quality control line simultaneously generate phosphorescent signals, a positive reaction result indicates that the sample contains a target substance to be detected; when the detection line does not generate a phosphorescent signal and the quality control line generates a phosphorescent signal, a negative reaction result is obtained, and the result indicates that the sample does not contain the target object to be detected.

The detection device can be used in the detection fields of disease diagnosis, drunk driving test, drug sucking condition, hormone condition analysis and the like, and meets the requirement of rapid sampling and rapid detection. The sample type detectable by the detection device can be urine, saliva, sample lysate and the like.

Claims

1. A detection device, comprising a sample collector and a detection box, characterized in that: sample collector includes the sampling probe, the handle and the connecting portion between sampling probe and handle, detect the box including accomodating chamber and buckle, connecting portion of sample collector are including the narrow wedge structure down of width, the buckle that detects the box includes a pair of pothook of inversion, should be relative each other inwards to the hook point of pothook, after putting into the taking in chamber of detecting the box with the sampling probe of sample collector, the wedge structure of sample collector extrudees the buckle downwards, make this pothook to invert outwards open, after the wedge structure passes through the hook point, the pothook kick-backs up and hooks the wedge structure.

2. The detecting device for detecting the rotation of a motor rotor as claimed in claim 1, wherein the wedge-shaped structure comprises at least one inclined surface or an arc surface, and the inclined surface or the arc surface guides the clamping hook to be outwards opened when the wedge-shaped structure downwards presses the clamping hook.

3. The detection apparatus according to claim 2, wherein at least a lower shape of the cross-section of the wedge-shaped structure comprises: inverted trapezoids, inverted triangles, combinations of inverted trapezoids and rectangles, semi-circles or approximate semi-circles.

4. The detecting device for detecting the rotation of a motor rotor as claimed in claim 1, wherein the cross section of the wedge-shaped structure is in an inverted convex shape.

5. The device as claimed in any one of claims 1 to 4, wherein the width of the base of the wedge-shaped structure is less than the distance between the two tips of the hooks.

6. The probe apparatus of claim 5, wherein the width of the base of the wedge structure is less than one-half of the width of the top thereof.

7. The testing device of claim 1, wherein the testing cassette comprises a second latch for latching the sampling tip of the sample collector after the sampling tip of the sample collector is placed in the receiving cavity of the testing device.

8. The testing device of claim 7, wherein the second catch is disposed within a receiving cavity of the testing device.

9. The testing device according to claim 8, wherein the end of the second latch has a protrusion, the sampling head of the sample collector has a recess, and the protrusion of the second latch is engaged with the recess of the sampling head of the sample collector when the sampling head of the sample collector is placed in the receiving cavity of the testing cassette.