CN115074409A - Micromolecule active peptide separation and purification system based on organic animal and plant raw materials - Google Patents
Micromolecule active peptide separation and purification system based on organic animal and plant raw materials Download PDFInfo
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- CN115074409A CN115074409A CN202210989647.6A CN202210989647A CN115074409A CN 115074409 A CN115074409 A CN 115074409A CN 202210989647 A CN202210989647 A CN 202210989647A CN 115074409 A CN115074409 A CN 115074409A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
Abstract
The invention provides a small molecule active peptide separation and purification system based on organic animal and plant raw materials, which comprises a detection module, a storage module, a purification module and a reflux module, wherein the storage module is used for storing the raw materials after enzymolysis, the detection module is used for detecting the purification state of the purification module, and the purification module is used for purifying the animal and plant raw materials after enzymolysis; the backflow module is used for returning the clarified liquid purified by the purification module at the previous time to the purification module, and purifying the clarified liquid again by the purification module to obtain clean clarified liquid. According to the invention, the purification detection unit and the purification module are mutually matched, so that the purification module dynamically adjusts the times of purification and filtration according to the data of the purification detection unit, and the obtained clarified liquid is ensured to be cleaner.
Description
Technical Field
The invention relates to the technical field of peptide or protein preparation equipment, in particular to a small molecule active peptide separation and purification system based on organic animal and plant raw materials.
Background
The small molecule active peptide from different organic animal and plant natural resources has multiple functions of pertinently supplementing organic tissue protein, enhancing the immunity of the organism, resisting tumor, reducing blood pressure, resisting oxidation and the like, has the advantages of good membrane permeability, strong pharmacodynamic activity, high oral bioavailability, rapid and sufficient digestion, transportation and absorption and synthesis of organism tissues and the like, and particularly has the following advantages of research and development and marketing of peptide drugs for resisting tumor, reducing blood pressure and the like, so that the small molecule active peptide, particularly the food active peptide, is more and more concerned by people, and the active small molecule peptide drug which is easy to absorb and has small toxic and side effects or functional food of reinforced nutritional supplements is researched and developed.
The small molecule active peptide of organic animal and plant raw material includes such as bovine bone collagen peptide, ovalbumin peptide or thymus protein peptide (calf thymus extract) etc., wherein the bovine bone collagen peptide is present in bovine bone, and these substances are extracted by physical method, chemical method because they cannot be extracted rapidly in bone; the majority of ovalbumin peptides are accomplished by the following steps: egg albumen → alkali degreasing → enzymolysis → filtration → decolorization → desalination → concentration → spray drying, which uses a large amount of alkali to degrease, not only brings serious environmental pollution, but also destroys the protein component; the extraction of the thymosin generally comprises the processes of histiocyte disruption, centrifugal separation, ultrafiltration, concentration and the like, and the intellectualization of the extraction and separation process is not high. The foregoing discussion of the background art is intended only to facilitate an understanding of the present invention. This discussion is not an acknowledgement or admission that any of the material referred to is part of the common general knowledge.
The invention aims to solve the problems of poor detection precision, poor purification means, low efficiency, low intelligence degree, incapability of continuous production and the like in the field.
Disclosure of Invention
The invention aims to provide a system for separating and purifying small molecule active peptide based on organic animal and plant raw materials aiming at the defects.
In order to overcome the defects of the prior art, the invention adopts the following technical scheme:
a separation and purification system of small molecule active peptide based on organic animal and plant raw materials comprises a detection module, a storage module, a purification module and a reflux module,
the storage module is used for storing the raw materials after enzymolysis, and the detection module is used for detecting the purification state of the purification module;
the purification module is used for purifying the animal and plant raw materials subjected to enzymolysis;
the backflow module is used for returning the clarified liquid purified by the purification module at the previous time to the purification module, and enabling the purification module to purify the clarified liquid at the previous time again so as to obtain clean clarified liquid;
the detection module comprises a purification detection unit and an evaluation unit, the purification detection unit is used for detecting the purification state of the purification module, the evaluation unit evaluates the purification state according to the data of the purification detection unit, and the purification detection unit is arranged on the periphery of the purification module so as to detect the clarified liquid in the purification process of the purification module;
the purification detection unit comprises a reference light sensor, a light source, a transmission light sensor, a first fixed seat, a second fixed seat and a rotating component, wherein the transmission light sensor and the reference light sensor are arranged on the first fixed seat at the same time, the light source is arranged on the second fixed seat, and the rotating component adjusts the positions of the first fixed seat and the second fixed seat;
the light source and the reference light sensor are respectively arranged in a first detection cavity, a second detection cavity and a third detection cavity of the purification module, and are positioned at two sides of the first detection cavity, the second detection cavity and the third detection cavity and are arranged opposite to each other;
the evaluation unit calculates the light intensity mean value T of the first detection cavity, the second detection cavity and the third detection cavity by scanning the reference light sensor and the transmission light sensor once according to the following formula n ：
In the formula, light i The light intensity value of the ith scanning point meets the following conditions:
in the formula, ML i When the light source at the ith scanning point irradiates the detection cavity, the transmitted light sensor receives the illumination intensity signal value JL i The illumination intensity signal value, KT, received by the reference light sensor when the light source at the ith scanning point irradiates the detection cavity 0 The value of the illumination intensity signal received by the transmitted light sensor when the light source is not illuminating the detection cavity, KB 0 When the light source does not irradiate the detection cavity, the Tran _ Zero is the maximum transmitted light intensity of the light source directly scattered on the detection cavity, and the value is determined by the adopted light source and the material of the detection cavity;
and according to the light intensity mean value T of each scanning n Calculate the overall average S of 20 scans T ：
The evaluation unit is used for evaluating the light intensity mean value T of each scanning n And the total average S of 20 scans T Calculating Clean index Clean n ：
In the formula, λ is an adjustment index whose value is related to the rotational deviation of the rotating member, and satisfies:
delta e is the maximum allowable position deviation value of the rotating member, and the value is directly determined according to the parameters of the rotating member;
if Clean index Clean n If the purification index is lower than the set cleaning monitoring threshold, the reflux module is triggered to carry out multiple times of rotary purification on the clarified liquid purified by the purification module for the previous time in the purification module until the purification index is Clean n After the cleanness monitoring threshold is met, the decolorization and purification process is carried out。
Optionally, the first detection chamber, the second detection chamber, and the third detection chamber are respectively configured with one rotating member, the rotating member includes a rotating track, a set of rotating seats, a limiting ring, and a rotating driving mechanism, the rotating track is disposed around the outer side of each detection chamber, the rotating track is coaxially disposed with the corresponding detection chamber, a set of rotating seats is slidably connected to one rotating track, and drives a set of rotating seats to slide along the rotating track based on the rotating driving mechanism, and the limiting ring is configured to limit a distance between the set of rotating seats;
wherein, a set of fixing base sets up respectively on a set of rotate the seat.
Optionally, the storage module includes a storage unit and a support unit, the storage unit is used for storing the raw material subjected to enzymolysis, and the support unit is used for supporting the storage unit;
the storage unit comprises a transparent storage tank and a cleaning component, wherein the storage tank is used for storing raw materials subjected to enzymolysis, and the cleaning component is used for cleaning the used storage tank;
wherein, it includes a plurality of cleaning head, booster pump, support ring and connecting tube to wash the component, each the cleaning head is along the equidistant distribution of length direction of support ring, the booster pump is used for carrying out the pressure boost to the washing liquid of supply to transmit pressurized washing liquid to each through connecting tube on the cleaning head.
Optionally, the purification module includes a centrifugation unit, a secondary filtration unit, and a tertiary filtration unit, the centrifugation unit centrifugalizes raw materials after enzymolysis to filter out impurities to obtain a primary clarified liquid with less impurities, the secondary filtration unit is switched to when the primary clarified liquid does not reach a cleaning monitoring threshold value, the secondary filtration unit is used for carrying out secondary filtration on the primary clarified liquid after centrifugation by the centrifugation unit to obtain a secondary clarified liquid with higher cleanliness, the tertiary filtration unit is switched to when the secondary clarified liquid does not reach the cleaning monitoring threshold value, and the tertiary filtration unit is used for carrying out tertiary filtration on the secondary clarified liquid which is filtered by the secondary filtration unit and does not reach the cleaning monitoring threshold value to obtain a tertiary clarified liquid with higher cleanliness;
the centrifugal unit stores the centrifuged primary clarified liquid in the first detection cavity, the secondary filtering unit stores the filtered secondary clarified liquid in the second detection cavity, and the tertiary filtering unit stores the filtered tertiary clarified liquid in the third detection cavity;
the centrifugal unit comprises a centrifugal net, a supporting seat and a centrifugal throwing component, the supporting seat is inwards sunken to form a storage cavity for storing the enzymolyzed raw materials, the centrifugal net is arranged on the bottom wall of the storage cavity to carry out solid-liquid separation on the enzymolyzed raw materials, and the centrifugal throwing component is in driving connection with the supporting seat to realize solid-liquid separation on clarified liquid and impurities in the storage cavity under the action of centrifugal force;
wherein, the centrifugal net with deposit the chamber and can dismantle and be connected.
Optionally, the backflow module includes a transmission unit, a first backflow pipeline, a second backflow pipeline, a first electronic control valve, and a second electronic control valve, the transmission unit is used for transmitting the primary clarified liquid which does not reach the cleaning monitoring threshold value from the centrifugal unit to the secondary filtering unit and transmitting the secondary clarified liquid which does not reach the cleaning monitoring threshold value from the second detection cavity corresponding to the secondary filtering unit to the tertiary filtering unit, so as to obtain a third clarified liquid with less impurities, the first return pipeline is arranged between the first detection cavity and the second filtering unit, so as to transmit the primary clarified liquid in the first detection cavity to a second detection cavity of the secondary filtering unit, the second return pipeline is arranged between the second detection cavity and the third filtering unit so as to transmit the secondary clarified liquid in the second detection cavity to the third detection cavity;
the first electronic control valve is used for controlling the on-off of the first backflow pipeline, and the second electronic control valve is used for controlling the on-off of the second backflow pipeline.
Optionally, the secondary filtering unit includes a first pressurizing member, a first filtering membrane, a first accommodating tank for storing the primary clarified liquid by a user, and a second detection chamber, a bottom wall of the first accommodating tank is provided with a first passage slit for the primary clarified liquid to pass through, wherein the first filtering membrane is arranged on the bottom wall of the first accommodating tank to filter impurities in the primary clarified liquid, and the first pressurizing member pressurizes the first accommodating tank, so that the primary clarified liquid can be rapidly discharged to obtain the secondary clarified liquid;
wherein, the second detection chamber is used for storing the secondary clarified liquid obtained by filtering.
Optionally, if the secondary clarified liquid filtered by the secondary filtering unit does not reach the cleaning monitoring threshold, the secondary clarified liquid is returned to the tertiary filtering unit through the backflow module to be filtered, so as to obtain the tertiary clarified liquid.
Optionally, the secondary filtration unit is disposed directly below the centrifugal unit.
Optionally, the filterable mesh number of the centrifugal screen is selected according to the nature of the filtered enzymatic raw material.
Optionally, the first detection cavity, the second detection cavity and the third detection cavity are all of the same type.
The beneficial effects obtained by the invention are as follows:
1. the purification detection unit is matched with the purification module, so that the purification module dynamically adjusts the times of purification and filtration according to the data of the purification detection unit, and the obtained clarified liquid is ensured to be cleaner;
2. the backflow module is matched with the purification detection unit, so that the backflow module flows unqualified clarified liquid back to the purification module for multiple times of filtration according to the result of the purification detection unit, and the intelligence of the purification process is greatly improved;
3. detecting the clarified liquid through a detection module to obtain the purification state of the clarified liquid;
4. through the mutual matching of the secondary filtering unit and the tertiary filtering unit, the clarified liquid is promoted to be purified gradually, the finally formed clarified liquid is ensured to reach the set standard, and the high efficiency and the accuracy of the purification of the whole system are also improved;
5. the position of the vibration reduction part is adjusted through the adjusting component, so that the distance between the vibration reduction part and the supporting seat can be adjusted, and the supporting seat is prevented from overturning or overflowing due to overlarge shaking.
Drawings
The invention will be further understood from the following description in conjunction with the accompanying drawings. The components in the figures are not necessarily to scale, emphasis instead being placed upon illustrating the principles of the embodiments. Like reference numerals designate corresponding parts throughout the different views.
FIG. 1 is an overall block diagram of the present invention.
Fig. 2 is a schematic structural diagram of a first detection chamber and a purification detection unit according to the present invention.
Fig. 3 is a schematic sectional view of fig. 3 at a-a.
Fig. 4 is a schematic structural diagram of a purification detection unit according to the present invention.
Fig. 5 is a schematic view of a detection scenario of the first detection chamber and the purification detection unit according to the present invention.
Fig. 6 is an enlarged schematic view of fig. 5 at B.
FIG. 7 is a schematic cross-sectional view of a storage module and centrifuge unit of the present invention.
Fig. 8 is an enlarged schematic view at C in fig. 7.
FIG. 9 is a schematic cross-sectional view of a secondary filtration unit of the present invention.
FIG. 10 is a schematic cross-sectional view of a triple filtration unit of the present invention.
Fig. 11 is a schematic structural view of the damping member, the adjustment member and the support seat of the present invention.
FIG. 12 is a schematic structural diagram of a storage module, a centrifugal unit, a secondary filtration unit and a tertiary filtration unit according to the present invention.
The reference numbers illustrate: 1. a first detection chamber; 2. a limiting ring; 3. a rotating seat; 4. rotating the rail; 5. a light source; 6. a transmitted light sensor; 7. a reference light sensor; 8. an adjusting lever; 9. a damping spring; 10. a damper rod; 11. connecting a pipeline; 12. raw materials for enzymolysis; 13. fixing the rod; 14. a swinging seat; 15. a first pressure applying pump; 16. a first pressure applying pipe; 17. a first holding tank; 18. a second detection chamber; 19. a first filter membrane; 20. a second holding tank; 21. a second filter membrane; 22. a third detection chamber; 23. a second pressure applying pump; 24. a second pressure applying pipe; 25. a supporting seat; 26. a support ring; 27. a cleaning head; 28. a drive shaft; 29. a centrifugal net; 30. a contact plate.
Detailed Description
The following embodiments are provided to illustrate the present invention by specific examples, and those skilled in the art will be able to understand the advantages and effects of the present invention from the disclosure of the present specification. The invention is capable of other and different embodiments and its several details are capable of modification in various other respects, all without departing from the spirit and scope of the present invention. The drawings of the present invention are for illustrative purposes only and are not intended to be drawn to scale. The following embodiments will further explain the related art of the present invention in detail, but the disclosure is not intended to limit the scope of the present invention.
The first embodiment.
According to the embodiments shown in fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, fig. 6, fig. 7, fig. 8, fig. 9, fig. 10, fig. 11 and fig. 12, the present embodiment provides a system for separating and purifying small molecule active peptide based on organic animal and plant raw materials, the system for separating and purifying active peptide comprises a detection module, a storage module, a purification module and a reflux module,
the storage module is used for storing the raw materials after enzymolysis, and the detection module is used for detecting the purification state of the purification module;
the purification module is used for purifying the animal and plant raw materials subjected to enzymolysis;
the backflow module is used for returning the clarified liquid purified by the purification module at the previous time to the purification module, and enabling the purification module to purify the clarified liquid at the previous time again so as to obtain clean clarified liquid;
the active peptide separation and purification system also comprises a processor, wherein the processor is respectively connected with the detection module, the storage module, the purification module and the reflux module in a control way and is used for carrying out centralized control on the detection module, the storage module, the purification module and the reflux module based on the processor;
after the raw materials are subjected to enzymolysis, the raw materials are stored in the storage module and transferred to the purification module to carry out purification treatment on the raw materials subjected to enzymolysis, so that clear liquid with higher purity is obtained;
optionally, the storage module includes a storage unit and a support unit, the storage unit is used for storing the raw material 12 subjected to enzymolysis, and the support unit is used for supporting the storage unit;
the storage unit comprises a transparent storage tank and a cleaning component, wherein the storage tank is used for storing raw materials subjected to enzymolysis, and the cleaning component is used for cleaning the used storage tank;
wherein the cleaning member comprises a plurality of cleaning heads 27, a booster pump, a support ring 26 and a connecting pipeline 11, each cleaning head 27 is distributed at equal intervals along the length direction of the support ring 26, the booster pump is used for boosting the supplied cleaning liquid and transmitting the boosted cleaning liquid to each cleaning head 27 through the connecting pipeline 11;
the storage tank is cleaned through the cleaning component, so that pollution caused by residual liquid left in the previous time can be effectively prevented;
after the raw material 12 subjected to enzymolysis in the storage module enters the purification module, impurities and liquid in the raw material are separated through the purification module;
optionally, the purification module includes a centrifugal unit, a secondary filtration unit and a tertiary filtration unit, the centrifugal unit centrifugalizes raw materials after enzymolysis to filter out impurities to obtain a primary clarified liquid with less impurities, the primary clarified liquid is transferred to the secondary filtration unit when the primary clarified liquid does not reach a cleaning monitoring threshold value, the secondary filtration unit is used for performing secondary filtration on the primary clarified liquid after the centrifugation by the centrifugal unit to obtain a secondary clarified liquid with higher cleanliness, the secondary clarified liquid is transferred to the tertiary filtration unit when the secondary clarified liquid does not reach the cleaning monitoring threshold value, and the tertiary filtration unit is used for performing tertiary filtration on the secondary clarified liquid which is filtered by the secondary filtration unit and does not reach the cleaning monitoring threshold value to obtain a tertiary clarified liquid with higher cleanliness;
the centrifugal unit stores the centrifuged primary clarified liquid in the first detection cavity, the secondary filtering unit stores the filtered secondary clarified liquid in the second detection cavity, and the tertiary filtering unit stores the filtered tertiary clarified liquid in the third detection cavity; the purification module further comprises a first detection cavity, and the first detection cavity is used for storing primary clarified liquid after centrifugal filtration of the centrifugal unit;
the centrifugal unit is arranged in a first detection cavity of the purification module to receive the raw materials which are subjected to enzymolysis in the storage tank, and the raw materials 12 subjected to enzymolysis are centrifuged to separate impurities from liquid, wherein in the embodiment, the raw materials can comprise animals and plants;
during the purification of the enzymolyzed raw material 12, introducing the raw material in the storage tank into the centrifugal unit, and centrifuging the enzymolyzed raw material by the centrifugal unit to extract a primary clarified liquid; wherein, the above-mentioned first clarified liquid contains impurities, so it needs to be filtered many times to obtain clarified liquid with higher purity;
the centrifugal unit comprises a centrifugal net 29, a supporting seat 25 and a centrifugal throwing component, wherein the supporting seat is inwards sunken to form a storage cavity for storing the enzymolyzed raw materials, the centrifugal net 29 is arranged on the bottom wall of the storage cavity to carry out solid-liquid separation on the enzymolyzed raw materials, and the centrifugal throwing component is in driving connection with the supporting seat to realize solid-liquid separation on clarified liquid and impurities in the storage cavity under the action of centrifugal force;
wherein the centrifugal net 29 is detachably connected with the storage cavity.
The centrifugal throwing component comprises a fixed rod 13, a transmission shaft 28, a throwing seat 14 and a throwing driving mechanism, wherein the fixed rod 13 is used for supporting the throwing seat 14, one end of the fixed rod 13 is connected with the throwing seat 14, the other end of the fixed rod 13 is connected with the inner wall of the first detection cavity, one end of the transmission shaft 28 is coaxially arranged with the supporting seat 25, and the other end of the transmission shaft 28 is in driving connection with the throwing driving mechanism so as to drive the supporting seat 25 and a centrifugal net 29 arranged on the supporting seat 25 to rotate, so that impurities of raw materials are separated from the clarified liquid by the raw materials under the action of centrifugal force; the swinging driving mechanism is arranged on the swinging seat 14;
the first detection cavity is used for storing centrifuged primary clarified liquid;
when the centrifugal throwing component separates impurities and liquid in the raw material 12 subjected to enzymolysis to obtain primary clarified liquid, detecting the primary clarified liquid through the detection module to obtain a purification state of the primary clarified liquid, and if the purity requirement cannot be met (the purity monitoring threshold value is not reached), performing secondary filtration and purification operation on the primary clarified liquid through the secondary filtration unit to obtain secondary clarified liquid;
optionally, the filterable mesh number of the centrifugal net 29 is selected according to the property of the filtered enzymolysis raw material 12, and the operator replaces or selects the matched centrifugal net 29 according to different purified materials;
the secondary filtering unit comprises a second detection cavity 18, and the second detection cavity 18 is used for storing secondary clarified liquid filtered by the secondary filtering unit; the third filtering unit comprises a third detection cavity 22, and the third detection cavity 22 is used for storing the third clarified liquid filtered by the third filtering unit;
the detection module comprises a purification detection unit and an evaluation unit, the purification detection unit is used for detecting the purification state of the purification module, the evaluation unit evaluates the purification state according to the data of the purification detection unit, and the purification detection unit is respectively arranged on the periphery of a first detection cavity 1 corresponding to a centrifugal unit of the purification module, a second detection cavity 18 corresponding to a secondary filtering unit of the purification module and a third detection cavity corresponding to a tertiary filtering unit of the purification module;
the purification detection unit comprises a reference light sensor 7, a light source 5, a transmission light sensor 6, a first fixed seat, a second fixed seat and a rotating component, wherein the transmission light sensor 6 and the reference light sensor 7 are arranged on the first fixed seat, the light source 5 is arranged on the second fixed seat, and the rotating component adjusts the positions of the first fixed seat and the second fixed seat; the light source 5 and the reference light sensor 7 are respectively arranged in a first detection cavity, a second detection cavity and a third detection cavity of the purification module, and are positioned at two sides of each detection cavity and are arranged opposite to each other;
in addition, the purification detection unit further includes a sliding member for adjusting a distance between the reference light sensor 7 and the transmitted light sensor 6, wherein the sliding member includes a sliding rail, a sliding seat slidably connected to the sliding rail, and a sliding driving mechanism provided on the sliding seat and driving the sliding seat to slide along an orientation of the sliding rail;
the sliding track is arranged on the first fixed seat, and the track direction of the sliding track is parallel to the direction of the rotating track 4 of the rotating component;
wherein, the transmission light sensor 6 is fixed on the first fixed seat; the reference light sensor 7 is arranged on the sliding seat and is driven by the sliding driving mechanism to slide along the direction of the sliding track, so that the distance between the reference light sensor 7 and the transmission light sensor 6 is adjusted;
the evaluation unit calculates the light intensity mean value T of the first detection cavity, the second detection cavity and the third detection cavity by scanning the reference light sensor and the transmission light sensor once according to the following formula n ：
In the formula, light i The light intensity value of the ith scanning point meets the following conditions:
in the formula, ML i When the light source at the ith scanning point irradiates the first detection cavity, the second detection cavity and the third detection cavity, the transmitted light sensor receives an illumination intensity signal value JL i When the light source at the ith scanning point irradiates the first detection cavity, the second detection cavity and the third detection cavity, the reference light sensor receives an illumination intensity signal value KT 0 A value of an illumination intensity signal received by the transmitted light sensor when the light source is not irradiated in the first detection chamber, the second detection chamber and the third detection chamber, KB 0 When the light source does not irradiate the first detection cavity, the second detection cavity and the third detection cavity, the Tran _ Zero is the maximum transmission light intensity of the light source directly scattered on the detection cavity, and the value is determined by the adopted light source and the materials of the first detection cavity, the second detection cavity and the third detection cavity;
and according to the light intensity mean value T of each scanning n Calculate the Total average S of 20 scans T ：
The evaluation unit is used for evaluating the light intensity average value T of each scanning n And the total average S of 20 scans T Calculating Clean index Clean n ：
In the formula, λ is an adjustment index whose value is related to the rotational deviation of the rotating member, and satisfies:
delta e is the maximum allowable position deviation value of the rotating member, and the value is directly determined according to the inherent parameters of the rotating member;
if Clean index Clean n If the purification index is lower than the set cleaning monitoring threshold, the reflux module is triggered to carry out multiple times of rotary purification on the clarified liquid purified by the purification module for the previous time in the purification module until the purification index is Clean n And (4) after the cleanness monitoring threshold is met, entering a decoloring and purifying process.
The set cleaning monitoring threshold is set by an operator according to the purification degree of the processed raw material, which is well known to those skilled in the art, and thus, in this embodiment, the details are not repeated;
it should be noted that the decolorization and purification process is realized by ultrafiltration membrane technology, which is a technical means well known to those skilled in the art, and those skilled in the art can query related technical manuals to know the technology, and thus details are not repeated in this embodiment;
optionally, the first detection cavity, the second detection cavity and the third detection cavity are respectively configured with one rotating member, each rotating member includes a rotating track 4, a set of rotating seats 3, a limiting ring 2 and a rotating driving mechanism, the outer side of each detection cavity is surrounded by the rotating track, the rotating track is coaxially arranged with the corresponding detection cavity, a set of rotating seats 3 is slidably connected with one rotating track 4, the rotating seats are driven to slide along one rotating track 4 based on the rotating driving mechanism, and the limiting ring 2 is used for limiting the distance between the rotating seats 3; wherein, a group of the fixed seats (a first fixed seat and a second fixed seat) is respectively arranged on a group of the rotating seats 3;
the limiting ring 2 is connected with the outer walls of the rotating seats 3, so that the rotating seats 3 are always arranged at opposite positions, and light rays emitted by the light source to the first detection cavity, the second detection cavity and the third detection cavity can be sensed by the reference light sensor 7 and the transmission light sensor 6;
optionally, the secondary filtering unit is configured to receive the primary clarified liquid separated by the centrifuging unit, and perform secondary filtering on the primary clarified liquid to obtain a secondary clarified liquid;
it is worth noting that when the secondary clarified liquid obtained after being filtered by the secondary filtering unit is subjected to clean index clearn detection by a purity detection unit, if the clean monitoring threshold value does not reach the clean monitoring threshold value, the secondary clarified liquid filtered by the secondary filtering unit is subjected to backflow transportation to the tertiary filtering unit by a backflow module to obtain a tertiary clarified liquid, wherein the tertiary filtering unit is filtered by a filtering membrane with a better filtering effect;
the secondary filtering unit comprises a first pressurizing component, a first filtering membrane 19, a first accommodating tank 17 for storing the primary clarified liquid by a user, and a second detecting cavity 18, wherein a first passing seam for the primary clarified liquid to pass is arranged on the bottom wall of the first accommodating tank 17, the first filtering membrane 19 is arranged on the bottom wall of the first accommodating tank 17 to filter impurities in the primary clarified liquid, the first pressurizing component pressurizes the first accommodating tank 17 to enable the primary clarified liquid to be rapidly discharged to obtain secondary clarified liquid, and the second detecting cavity 18 is used for storing the secondary clarified liquid obtained by filtering;
the second detection cavity 18 is used for receiving the secondary clarified liquid filtered by the first holding tank 17, and the purity detection module is arranged on the periphery of the second detection cavity 18;
the first pressurizing means comprises a first pressurizing pump 15, the first pressurizing pump 15 is used for pressurizing the first accommodating tank 17 to form a micro pressure in the first accommodating tank 17, and the primary clarified liquid can rapidly pass through the first filtering membrane 19 of the first accommodating tank 17;
optionally, if the secondary clarified liquid filtered by the secondary filtering unit does not reach the cleaning monitoring threshold, returning the secondary clarified liquid to the tertiary filtering unit through the backflow module for filtering to obtain a tertiary clarified liquid;
optionally, the secondary filtering unit is arranged right below the centrifugal unit to receive the primary clarified liquid centrifuged by the centrifugal unit and perform secondary filtering on the primary clarified liquid;
in addition, the tertiary filtration unit comprises a second pressurizing member, a second filtration membrane 21, a second containing tank 20 for storing the secondary clarified liquid by a user, and the third detection chamber 22, wherein a second through seam for the secondary clarified liquid to pass through is arranged on the bottom wall of the second containing tank 20, the second filtration membrane 21 is arranged on the bottom wall of the second containing tank 20 to filter impurities in the secondary clarified liquid, and the second pressurizing member pressurizes the second containing tank 20, so that the secondary clarified liquid can be rapidly discharged from the second containing tank 20 to obtain the tertiary clarified liquid;
the third detection cavity 22 is used for receiving the filtered tertiary clarified liquid from the second holding tank 20, and the purity detection module is arranged on the periphery of the third detection cavity 22;
wherein the second pressurizing means comprises a second pressurizing pump 23 and a second pressurizing pipeline 24, the second pressurizing pump 23 pressurizes the second holding tank 20 through the second pressurizing pipeline 24 to form a micro-pressure in the second holding tank 20, so that the secondary clarified liquid can rapidly pass through the second filtering membrane 21;
through the mutual matching of the secondary filtering unit and the tertiary filtering unit, the clarified liquid is promoted to be purified gradually, the finally formed clarified liquid is ensured to reach the set standard, namely the cleaning monitoring threshold value is reached, and the purification efficiency and accuracy of the whole system are also improved;
optionally, the backflow module includes a transmission unit, a first backflow pipeline, a second backflow pipeline, a first electronic control valve, and a second electronic control valve, the transmission unit is used for transmitting the primary clarified liquid which does not reach the cleaning monitoring threshold value from the centrifugal unit to the secondary filtering unit and transmitting the secondary clarified liquid which does not reach the cleaning monitoring threshold value from the second detection cavity corresponding to the secondary filtering unit to the tertiary filtering unit, so as to obtain a third clarified liquid with less impurities, the first return pipeline is arranged between the first detection cavity and the second filtering unit, so as to transmit the primary clarified liquid in the first detection cavity to a second detection cavity of the secondary filtering unit, the second return pipeline is arranged between the second detection cavity and the third filtering unit so as to transmit the secondary clarified liquid in the second detection cavity to the third detection cavity;
the first electronic control valve is used for controlling the on-off of the first backflow pipeline, and the second electronic control valve is used for controlling the on-off of the second backflow pipeline.
It is worth noting that if the secondary clarified liquid after the filtration of the secondary filtration unit meets the cleaning monitoring threshold, the process is directly returned to the next decolorization and purification process; if the secondary clarified liquid after being filtered by the secondary filtering unit does not meet the cleaning monitoring threshold, turning to the tertiary filtering unit for secondary purification, and directly turning to the next decoloring and purifying process when the tertiary clarified liquid after being filtered by the tertiary filtering unit meets the cleaning monitoring threshold;
in addition, the number of purification is not limited to three, and the technicians in the field can adjust the purification in a self-adaptive manner according to actual needs or production requirements, so that the details are not repeated;
whether the secondary clarified liquid filtered by the secondary filtering unit meets a cleaning monitoring threshold value is determined according to the evaluation result of the purification detection unit;
it should be noted that, in the purification process, the process of preparing the small molecule active peptide from the raw material 12 subjected to enzymolysis, the filtration purification process may be more than three times, and those skilled in the art can select or adjust the filtration times by analogy, which is well known to those skilled in the art, and thus, in this embodiment, the details are not repeated.
The second embodiment.
This embodiment should be understood to include at least all the features of any one of the embodiments described above and further improve on the same, as shown in fig. 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11 and 12, and further characterized in that the centrifugal unit further includes a liquid level detector for detecting the liquid level carried in the support base 25 to obtain the liquid level data in the support base 25, a vibration damping member and an adjusting member;
the vibration reduction component is used for reducing vibration of the vibration quantity of the centrifugal swinging component so as to prevent the centrifugal swinging component from swinging too much to cause overturning and overflowing; the adjusting component is used for adjusting the position of the vibration damping component;
the vibration reduction component and the adjusting component perform vibration reduction on the supporting seat 25 according to the liquid level height data detected by the liquid level detecting piece, so as to ensure that raw materials subjected to enzymolysis in the supporting seat 25 do not overflow due to the action of centrifugal force;
the vibration damping component comprises a fixing ring, a plurality of vibration damping springs 9, a plurality of vibration damping seats and a plurality of contact plates, wherein each vibration damping spring 9 is respectively embedded on the vibration damping seat, one end of each vibration damping spring 9 is connected with a rod body of the vibration damping seat, and the other end of each vibration damping spring 9 is suspended and extends out towards one side far away from the vibration damping seat and forms a vibration damping part with one side end face of the contact plate;
the vibration reduction parts are distributed at equal intervals along the periphery of the supporting seat 25 so as to reduce vibration and limit the supporting seat 25;
in the process of vibration damping and limiting of the support seat 25 by the vibration damping member, the contact plate is in contact with the outer wall of the support seat 25, so that the support seat 25 cannot excessively deviate to cause overturning in the rotating process;
the outer wall of the supporting seat 25 is provided with a plurality of ribs, and the ribs are distributed at equal intervals along the periphery of the supporting seat 25;
in addition, the contact plate is made of silicon rubber and is connected with ribs on the outer wall of the supporting seat 25 so as to buffer the vibration of the supporting seat 25;
the adjusting component comprises an adjusting rod 8, an adjusting driving mechanism and an extension detecting piece, the extension detecting piece is used for detecting the extension length of the adjusting rod 8, one end of the adjusting rod 8 is connected with the damping rod 10 to adjust the position of the damping rod 10, and the other end of the adjusting rod 8 is in driving connection with the adjusting driving mechanism to drive the adjusting rod 8 to extend and retract;
in this embodiment, the adjusting rod 8 is arranged to be telescopic, and is driven by the adjusting driving mechanism to realize telescopic action;
the position of the vibration damping part is adjusted by the adjusting member, so that the distance between the vibration damping part and the support seat 25 can be adjusted, and the support seat 25 is prevented from overturning or overflowing due to excessive shaking.
The disclosure is only a preferred embodiment of the invention, and is not intended to limit the scope of the invention, so that all equivalent technical changes made by using the contents of the specification and the drawings are included in the scope of the invention, and further, the elements thereof can be updated as the technology develops.
Claims (10)
1. A separation and purification system of small molecule active peptide based on organic animal and plant raw materials is characterized in that the separation and purification system of active peptide comprises a detection module, a storage module, a purification module and a reflux module,
the storage module is used for storing the raw materials subjected to enzymolysis, the detection module is used for detecting the purification state of the purification module, and the purification module is used for purifying the animal and plant raw materials subjected to enzymolysis;
the backflow module is used for returning the clarified liquid purified by the purification module at the previous time to the purification module, and enabling the purification module to purify the clarified liquid at the previous time again so as to obtain clean clarified liquid;
the detection module comprises a purification detection unit and an evaluation unit, the purification detection unit is used for detecting the purification state of the purification module, the evaluation unit evaluates the purification state according to the data of the purification detection unit, and the purification detection unit is arranged on the periphery of the purification module so as to detect the clarified liquid in the purification process of the purification module;
the purification detection unit comprises a reference light sensor, a light source, a transmission light sensor, a first fixed seat, a second fixed seat and a rotating component, wherein the transmission light sensor and the reference light sensor are arranged on the first fixed seat at the same time, the light source is arranged on the second fixed seat, and the rotating component adjusts the positions of the first fixed seat and the second fixed seat;
the light source and the reference light sensor are respectively arranged in a first detection cavity, a second detection cavity and a third detection cavity of the purification module, and are positioned at two sides of the first detection cavity, the second detection cavity and the third detection cavity and are arranged opposite to each other;
the evaluation unit calculates the light intensity mean value T of the first detection cavity, the second detection cavity and the third detection cavity by scanning the reference light sensor and the transmission light sensor once according to the following formula n ：
In the formula, light i The light intensity value of the ith scanning point meets the following conditions:
in the formula, ML i When the light source at the ith scanning point irradiates the first detection cavity, the second detection cavity and the third detection cavity, the transmitted light sensor receives an illumination intensity signal value JL i When the light source at the ith scanning point irradiates the first detection cavity, the second detection cavity and the third detection cavity, the reference light sensor receives an illumination intensity signal value KT 0 A value of an illumination intensity signal received by the transmitted light sensor when the light source is not irradiated in the first detection chamber, the second detection chamber and the third detection chamber, KB 0 The light received by the reference light sensor is not irradiated by the light source in the first detection cavity, the second detection cavity and the third detection cavityAccording to the intensity signal value, Tran _ Zero is the maximum transmission light intensity of the light source directly scattered on the detection cavity, and the value is determined by the adopted light source and the materials of the first detection cavity, the second detection cavity and the third detection cavity;
and according to the light intensity mean value T of each scanning n Calculate the Total average S of 20 scans T ：
The evaluation unit is used for evaluating the light intensity mean value T of each scanning n And the total average S of 20 scans T Calculating Clean index Clean n ：
Wherein λ is an adjustment index whose value is related to the rotational deviation of the rotating member, and satisfies:
delta e is the maximum allowable position deviation value of the rotating member, and the value is directly determined according to the parameters of the rotating member;
if Clean index Clean n If the purification index is lower than the set cleaning monitoring threshold, the reflux module is triggered to carry out multiple times of rotary purification on the clarified liquid purified by the purification module for the previous time in the purification module until the purification index is Clean n And (4) after the cleanness monitoring threshold is met, entering a decoloring and purifying process.
2. The system for separating and purifying small molecule active peptide based on organic animal and plant raw materials as claimed in claim 1, wherein the first detection chamber, the second detection chamber and the third detection chamber are respectively configured with one of the rotating members, the rotating member comprises a rotating track, a set of rotating seats, a limiting ring and a rotating driving mechanism, the rotating track is disposed around the outside of each detection chamber, the rotating track is disposed coaxially with the corresponding detection chamber, a set of the rotating seats is slidably connected with one of the rotating tracks, and drives a set of the rotating seats to slide along the rotating track based on the rotating driving mechanism, and the limiting ring is used for limiting the distance between a set of the rotating seats;
wherein, a set of fixing base sets up respectively on a set of rotate the seat.
3. The system for separating and purifying the small molecule active peptide based on the organic animal and plant raw materials as claimed in claim 2, wherein the storage module comprises a storage unit and a support unit, the storage unit is used for storing the enzymolytic raw materials, and the support unit is used for supporting the storage unit;
the storage unit comprises a transparent storage tank and a cleaning component, wherein the storage tank is used for storing raw materials subjected to enzymolysis, and the cleaning component is used for cleaning the used storage tank;
wherein, it includes a plurality of cleaning head, booster pump, support ring and connecting tube to wash the component, each the cleaning head is along the equidistant distribution of length direction of support ring, the booster pump is used for carrying out the pressure boost to the washing liquid of supply to transmit pressurized washing liquid to each through connecting tube on the cleaning head.
4. The system for separating and purifying small molecule active peptide based on organic animal and plant raw material as claimed in claim 3, it is characterized in that the purification module comprises a centrifugal unit, a secondary filtering unit and a tertiary filtering unit, the centrifugal unit centrifugalizes the raw materials after enzymolysis to filter out impurities to obtain primary clarified liquid with less impurities, when the primary clarified liquid does not reach the cleaning monitoring threshold value, the secondary clarified liquid is transferred to the secondary filtering unit, the secondary filtering unit is used for carrying out secondary filtering on the primary clarified liquid after being centrifuged by the centrifuging unit so as to obtain secondary clarified liquid with higher cleanliness, when the secondary clarified liquid does not reach the cleaning monitoring threshold, the secondary clarified liquid is transferred to the tertiary filtering unit, and the tertiary filtering unit is used for carrying out tertiary filtering on the secondary clarified liquid which is filtered by the secondary filtering unit and does not reach the cleaning monitoring threshold so as to obtain tertiary clarified liquid with higher cleanliness;
the centrifugal unit stores the centrifuged primary clarified liquid in the first detection cavity, the secondary filtering unit stores the filtered secondary clarified liquid in the second detection cavity, and the tertiary filtering unit stores the filtered tertiary clarified liquid in the third detection cavity; the centrifugal unit comprises a centrifugal net, a supporting seat and a centrifugal throwing component, the supporting seat is inwards sunken to form a storage cavity for storing the enzymolyzed raw materials, the centrifugal net is arranged on the bottom wall of the storage cavity to carry out solid-liquid separation on the enzymolyzed raw materials, and the centrifugal throwing component is in driving connection with the supporting seat to realize solid-liquid separation on clarified liquid and impurities in the storage cavity under the action of centrifugal force;
wherein, the centrifugal net is detachably connected with the storage cavity.
5. The system of claim 4, wherein the backflow module comprises a transmission unit, a first backflow pipeline, a second backflow pipeline, a first electronic control valve, and a second electronic control valve, the transmission unit is used for transmitting the primary clarified liquid which does not reach the cleanness monitoring threshold from a first detection cavity corresponding to the centrifugal unit to the secondary filtration unit, and transmitting the secondary clarified liquid which does not reach the cleanness monitoring threshold from a second detection cavity corresponding to the secondary filtration unit to the tertiary filtration unit, so as to obtain the tertiary clarified liquid with fewer impurities, the first backflow pipeline is arranged between the first detection cavity and the secondary filtration unit, so as to transmit the primary clarified liquid in the first detection cavity to a second detection cavity of the secondary filtration unit, the second return pipeline is arranged between the second detection cavity and the third filtering unit so as to transmit the secondary clarified liquid in the second detection cavity to the third detection cavity;
the first electronic control valve is used for controlling the on-off of the first backflow pipeline, and the second electronic control valve is used for controlling the on-off of the second backflow pipeline.
6. The system for separating and purifying small molecule active peptide based on organic animal and plant raw materials, as claimed in claim 5, wherein the secondary filtering unit comprises a first pressurizing member, a first filtering membrane, and a first holding tank for storing the primary clarified liquid by a user, a second detecting chamber, the bottom wall of the first holding tank is provided with a first passing slit for passing the primary clarified liquid, wherein the first filtering membrane is disposed on the bottom wall of the first holding tank for filtering impurities in the primary clarified liquid, the first pressurizing member pressurizes the first holding tank, so that the primary clarified liquid can be rapidly discharged to obtain the secondary clarified liquid;
wherein, the second detection chamber is used for storing the secondary clarified liquid obtained by filtering.
7. The system for separating and purifying the small-molecule active peptide based on the organic animal and plant raw materials as claimed in claim 6, wherein if the secondary clarified liquid filtered by the secondary filtering unit does not reach the cleanliness monitoring threshold, the secondary clarified liquid is returned to the tertiary filtering unit through the return module for filtering to obtain the tertiary clarified liquid.
8. The system for separating and purifying small molecule active peptide based on organic animal and plant raw materials as claimed in claim 7, characterized in that said secondary filtration unit is disposed right below said centrifugal unit.
9. The system for separating and purifying small molecule active peptide based on organic animal and plant raw materials as claimed in claim 8, wherein the filterable mesh number of the centrifugal net is selected according to the nature of the filtered raw materials for enzymolysis.
10. The system for separating and purifying the small molecule active peptide based on the organic animal and plant raw material as claimed in claim 9, wherein the first detection chamber, the second detection chamber and the third detection chamber are all of the same type.
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Citations (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012115783A (en) * | 2010-12-02 | 2012-06-21 | Toray Ind Inc | Method for washing separation membrane module for continuous fermentation and device for separating membrane for continuous fermentation |
| CN203613198U (en) * | 2013-11-18 | 2014-05-28 | 中国农业科学院农产品加工研究所 | Reaction system for preparing high molecular weight vegetable protein peptide |
| CN105628644A (en) * | 2015-12-21 | 2016-06-01 | 江苏大学 | Device and method for on-line monitoring of protein enzymolysis process based on in-situ real-time spectrum |
| CN106967785A (en) * | 2017-02-27 | 2017-07-21 | 江苏科技大学 | A kind of enzymatic isolation method produces the method for real-time monitoring of blood sugar reducing peptide process |
| CN207047133U (en) * | 2017-06-09 | 2018-02-27 | 河南工程学院 | A kind of membrane separation device of polypeptide classification separation |
| CN108107018A (en) * | 2017-11-13 | 2018-06-01 | 江苏大学 | A kind of macromolecular polypeptides based on gastro-intestinal digestion prepare INSITU REAL TIME |
| CN208454858U (en) * | 2018-06-19 | 2019-02-01 | 武汉天天好生物制品有限公司 | A kind of polypeptide preparing mechanism and polypeptide preparation system |
| CN109313419A (en) * | 2016-04-04 | 2019-02-05 | 贝林格尔·英格海姆Rcv两合公司 | The real-time monitoring of purifying products |
| CN208500816U (en) * | 2018-06-27 | 2019-02-15 | 德兰梅勒（北京）分离技术股份有限公司 | A kind of animal polypeptide refinement treatment system |
| CN208883897U (en) * | 2018-09-29 | 2019-05-21 | 河南柏裕植物免疫科技有限公司 | Novel intelligent plant immune protein peptides process units |
| CN209768840U (en) * | 2019-02-21 | 2019-12-13 | 山东大树达孚特膳食品有限公司 | Steam jet device for peptide process |
| CN112041663A (en) * | 2018-03-02 | 2020-12-04 | 建新公司 | Multivariate spectral analysis and monitoring for bio-manufacturing |
| CN113136331A (en) * | 2021-05-19 | 2021-07-20 | 王强强 | Processing equipment for extracting collagen and collagen extraction method |
| CN214142148U (en) * | 2020-11-25 | 2021-09-07 | 广西芊河源生物科技有限公司 | Technological platform for extracting polypeptide by enzyme method |
| WO2022036739A1 (en) * | 2020-08-19 | 2022-02-24 | 上海心光生物医药有限责任公司 | Fluid treatment method, and cycle treatment device and system |
| CN114106147A (en) * | 2020-11-19 | 2022-03-01 | 烟台朗迪生物科技有限公司 | Fish glue protein extraction device and extraction method thereof |
-
2022
- 2022-08-18 CN CN202210989647.6A patent/CN115074409B/en active Active
Patent Citations (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012115783A (en) * | 2010-12-02 | 2012-06-21 | Toray Ind Inc | Method for washing separation membrane module for continuous fermentation and device for separating membrane for continuous fermentation |
| CN203613198U (en) * | 2013-11-18 | 2014-05-28 | 中国农业科学院农产品加工研究所 | Reaction system for preparing high molecular weight vegetable protein peptide |
| CN105628644A (en) * | 2015-12-21 | 2016-06-01 | 江苏大学 | Device and method for on-line monitoring of protein enzymolysis process based on in-situ real-time spectrum |
| CN109313419A (en) * | 2016-04-04 | 2019-02-05 | 贝林格尔·英格海姆Rcv两合公司 | The real-time monitoring of purifying products |
| CN106967785A (en) * | 2017-02-27 | 2017-07-21 | 江苏科技大学 | A kind of enzymatic isolation method produces the method for real-time monitoring of blood sugar reducing peptide process |
| CN207047133U (en) * | 2017-06-09 | 2018-02-27 | 河南工程学院 | A kind of membrane separation device of polypeptide classification separation |
| CN108107018A (en) * | 2017-11-13 | 2018-06-01 | 江苏大学 | A kind of macromolecular polypeptides based on gastro-intestinal digestion prepare INSITU REAL TIME |
| CN112041663A (en) * | 2018-03-02 | 2020-12-04 | 建新公司 | Multivariate spectral analysis and monitoring for bio-manufacturing |
| CN208454858U (en) * | 2018-06-19 | 2019-02-01 | 武汉天天好生物制品有限公司 | A kind of polypeptide preparing mechanism and polypeptide preparation system |
| CN208500816U (en) * | 2018-06-27 | 2019-02-15 | 德兰梅勒（北京）分离技术股份有限公司 | A kind of animal polypeptide refinement treatment system |
| CN208883897U (en) * | 2018-09-29 | 2019-05-21 | 河南柏裕植物免疫科技有限公司 | Novel intelligent plant immune protein peptides process units |
| CN209768840U (en) * | 2019-02-21 | 2019-12-13 | 山东大树达孚特膳食品有限公司 | Steam jet device for peptide process |
| WO2022036739A1 (en) * | 2020-08-19 | 2022-02-24 | 上海心光生物医药有限责任公司 | Fluid treatment method, and cycle treatment device and system |
| CN114106147A (en) * | 2020-11-19 | 2022-03-01 | 烟台朗迪生物科技有限公司 | Fish glue protein extraction device and extraction method thereof |
| CN214142148U (en) * | 2020-11-25 | 2021-09-07 | 广西芊河源生物科技有限公司 | Technological platform for extracting polypeptide by enzyme method |
| CN113136331A (en) * | 2021-05-19 | 2021-07-20 | 王强强 | Processing equipment for extracting collagen and collagen extraction method |
Non-Patent Citations (2)
| Title |
|---|
| 徐玉兰等: "大豆分离蛋白的酶水解", 《应用化学》 * |
| 许学勤等: "《食品工厂机械与设备》", 30 November 2018, 中国轻工业出版社 * |
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