CN114736787A - Nucleic acid elution and detection device - Google Patents
Nucleic acid elution and detection device Download PDFInfo
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- CN114736787A CN114736787A CN202210649205.7A CN202210649205A CN114736787A CN 114736787 A CN114736787 A CN 114736787A CN 202210649205 A CN202210649205 A CN 202210649205A CN 114736787 A CN114736787 A CN 114736787A
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Abstract
The invention provides a nucleic acid elution and detection device, which relates to the technical field of nucleic acid detection and comprises the following components: the device comprises a sampling bottle, a centrifugal mechanism, a magnetic bead transfer mechanism and a heating mechanism, wherein the upper end of the sampling bottle is open, the sampling bottle is used for containing binding liquid, a sample and magnetic beads, and nucleic acid of the sample can be adsorbed by the magnetic beads in the binding liquid; the centrifugal mechanism is provided with a centrifugal station, the centrifugal station is used for arranging a chip, and the centrifugal mechanism is used for centrifuging the chip on the centrifugal station; the magnetic bead transferring mechanism is used for transferring the magnetic beads with the nucleic acids adsorbed in the sampling bottle to a sample adding port of the chip on the centrifugal station and eluting the nucleic acids on the magnetic beads at the sample adding port; the heating end of the heating mechanism can be contacted with and separated from the chip. The nucleic acid elution and detection device provided by the invention is simple to operate, needs few instruments and equipment, has low technical requirements, and meanwhile, has a simple structure and low production cost, so that the air microorganism detection is more convenient, rapid and easy to popularize.
Description
Technical Field
The invention relates to the technical field of nucleic acid detection, in particular to a nucleic acid elution and detection device.
Background
Microorganisms in human, animal and plant bodies and soil can be dispersed in the air by droplets, dust, and the like, so that the air contains a certain kind and number of microorganisms. There are generally no pathogenic microorganisms in the air theoretically, but aerosols of pathogenic microorganisms are often suspended in the air near hospitals, veterinary hospitals and livestock houses, and healthy people or animals are often infected by inhalation. Air contaminated by pathogenic microorganisms can often be a source or vehicle of contamination, causing epidemic disease. Therefore, the detection of airborne microorganisms is of great significance for the prevention and control of infectious diseases and the hygiene supervision and protection of the environment.
The DNA extraction technology is the basic technology of molecular biology research, is the first step of DNA inspection, and is also the most critical step. The magnetic bead method nucleic acid extraction technology adopts nanometer magnetic bead microbeads, and the surfaces of the magnetic bead microbeads are marked with a functional group which can perform adsorption reaction with nucleic acid. The magnetic bead method for extracting DNA can be widely applied to many fields of genome research, HPV detection, paternity test, archaeology and the like. Compared with the traditional method, the magnetic bead method for extracting DNA has the following advantages: the Chelex100 method, the organic method, the silicon dioxide method, the salting-out method and the like are simpler and more convenient, and the magnetic bead method is irreplaceable in the aspects of DNA purification, micro-detection, PCR amplification and the like.
The air microorganism sample collection is a preposed work of air microorganism detection, a cracking combination liquid is used as a sampling liquid, magnetic beads are added into the sampling liquid, air microorganisms can be cracked while the air microorganism sample is collected, nucleic acid of the air microorganisms is released and adsorbed onto the magnetic beads, the air microorganism sample collection and the nucleic acid extraction are synchronously carried out, and the whole process of the air microorganism detection is accelerated.
The microfluidic chip technology is also called as microfluidic chip technology, and can integrate basic operation units of sample preparation, reaction, separation, detection and the like in biological, chemical and medical analysis processes on a chip with a plurality of equally divided centimeters so that a controllable fluid can penetrate through the whole system to replace various functions of a conventional chemical or biological laboratory. At present, the microfluidic chip is widely applied in the field of microbial detection.
The existing air microorganism detection mainly depends on manual operation, extraction, elution, detection and the like of microorganism nucleic acid are completed by manually operating different instruments, such as a centrifugal machine, a heating device and the like, and the detection and elution are complex in operation, long in time consumption, many instruments and equipment are needed, the cost is high, and the technical requirement is high.
Disclosure of Invention
The invention aims to provide a nucleic acid elution and detection device, which aims to solve the problems in the prior art and can elute magnetic beads adsorbing air microorganism nucleic acid in a sampling bottle and detect the eluted nucleic acid; utilize this device can realize the semi-automatic operation of air microorganism detection, only need to put into this device with the sample of gathering and the required chip of detection, can accomplish the detection to air microorganism sample, and easy operation, required instrument and equipment are few, and technical requirement is low, and simultaneously, this device simple structure, and low in production cost, it is convenient quick more to make air microorganism detection, easily popularization, in addition, except air microorganism sample, this device also can be used to elute and detect the nucleic acid sample of this elution to other magnetic beads that are in the binding solution and adsorb that have nucleic acid sample, save the manual operation of nucleic acid elution and detection.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides a nucleic acid elution and detection device, which comprises:
the sampling bottle is open at the upper end and is used for containing a binding solution, a sample and magnetic beads, and nucleic acid of the sample can be adsorbed by the magnetic beads in the binding solution;
the centrifugal mechanism is provided with a centrifugal station, the centrifugal station is used for arranging a chip, and the centrifugal mechanism is used for centrifuging the chip on the centrifugal station;
the magnetic bead transferring mechanism is used for transferring the magnetic beads, which are adsorbed with the nucleic acids in the sampling bottle, to a sample adding opening of the chip on the centrifugal station and eluting the nucleic acids on the magnetic beads at the sample adding opening;
a heating mechanism, a heating end of the heating mechanism being capable of contacting and separating from the chip.
Preferably, the heating mechanism comprises a heating plate, a spring, a first electromagnet and a second electromagnet; the heating plate is arranged on one side of the chip on the centrifugal station, the spring is positioned on one side of the heating plate away from the chip, one end of the spring is fixedly connected with the heating plate, the other end of the spring is fixedly arranged, the first electromagnet is fixedly arranged on the surface of one side of the heating plate, which is provided with the spring, the second electromagnet is fixedly arranged on one side of the first electromagnet away from the heating plate, and a gap is formed between the second electromagnet and the first electromagnet;
when the second electromagnet and the first electromagnet are not electrified, the heating plate is contacted with the chip under the action of the elastic force of the spring; and under the state that the second electromagnet and the first electromagnet are electrified, the second electromagnet and the first electromagnet attract each other and the attraction can overcome the elasticity of the spring to attract the heating plate to be separated from the chip.
Preferably, the heating mechanism further comprises a base, the heating plate is arranged below the chip on the centrifugal station, the base is fixedly arranged below the heating plate, one end of the spring, far away from the heating plate, is fixedly arranged on the base, and the second electromagnet is also fixed on the base.
Preferably, the heating plate is annular, the heating mechanism comprises a plurality of springs, a plurality of first electromagnets and a plurality of second electromagnets, the springs are uniformly arranged on one side of the heating plate along the circumferential direction of the heating plate, the first electromagnets are uniformly arranged on one side of the heating plate along the circumferential direction of the heating plate, the second electromagnets and the first electromagnets are arranged in a one-to-one correspondence manner, a plurality of reaction chambers are arranged in the chip, orthographic projections of the reaction chambers of the chip on the centrifugal station on a horizontal plane are first projections, orthographic projections of the heating plate on the horizontal plane are second projections, and the first projections are completely located in the second projections;
the number of the first electromagnets is equal to that of the second electromagnets and that of the springs, and one first electromagnet and one second electromagnet are arranged in each spring.
Preferably, the magnetic bead transferring mechanism comprises a lifting driving mechanism, a horizontal linear driving mechanism, a fixing rod and a sleeve, the sleeve is a disposable article, the lifting driving mechanism is fixedly arranged, the horizontal linear driving mechanism is fixedly arranged at a lifting driving end of the lifting driving mechanism, the fixing rod is vertically arranged, the top end of the fixing rod is fixedly arranged at the horizontal driving end of the horizontal linear driving mechanism, a magnet for adsorbing magnetic beads is fixedly arranged at the bottom end of the fixing rod, one end of the sleeve is open, the other end of the sleeve is closed, the sleeve can be sleeved on the fixing rod from the bottom end of the fixing rod, the magnet can adsorb the magnetic beads through the sleeve, the horizontal linear driving mechanism can drive the fixing rod to move along a first straight line, the sampling bottles and the sample adding ports of the chips are arranged along a second straight line, the first line and the second line are parallel.
Preferably, the outer wall of the fixing rod is provided with a vertical sliding groove and a transverse sliding groove, the vertical sliding groove extends from the bottom end of the fixing rod to the top end of the fixing rod along the fixing rod, one end of the vertical sliding groove close to the top end of the fixing rod is a tail end, one end of the transverse sliding groove is communicated with the tail end of the vertical sliding groove, and the transverse sliding groove extends from the tail end of the vertical sliding groove along the circumferential direction of the fixing rod; the utility model discloses a slide assembly, including the casing pipe, the casing pipe is provided with the open-ended one end on the inner wall fixed be provided with the vertical spout with the slider of the width looks adaptation of horizontal spout, the casing pipe is followed when the bottom of dead lever is upwards established to the cover, the slider joint in the vertical spout and to the terminal of vertical spout removes, the slider removes to after the terminal of vertical spout, along the direction that horizontal spout extends rotates the casing pipe can make the slider slide to in the horizontal spout.
Preferably, the outer wall of the fixing rod is provided with two vertical sliding grooves and two transverse sliding grooves, one vertical sliding groove and one transverse sliding groove are correspondingly arranged, the other vertical sliding groove and the other transverse sliding groove are correspondingly arranged, and the two vertical sliding grooves are uniformly distributed along the circumferential direction of the fixing rod; two sliding blocks are arranged on the inner wall of the sleeve, and the two sliding blocks are respectively arranged corresponding to the two vertical sliding grooves.
Preferably, the bottom surface of chip is provided with certainly the bottom surface center of chip along a plurality of draw-in grooves of bottom surface radial extension, centrifugal mechanism includes centrifugal drive device, pivot and a plurality of kelly, the vertical setting of pivot, centrifugal drive device can drive the pivot is rotatory, kelly one end fixed set up in the pivot, the other end is along the perpendicular to the direction of pivot axis and to keeping away from the direction of pivot extends, one the kelly is corresponding to one the draw-in groove, each the kelly all can the joint with this the kelly is corresponding in the draw-in groove.
Preferably, a fan-shaped groove extending along a first circumference is formed in the side wall of the clamping groove, the center of the first circumference is located on the central line of the chip, the clamping rod is clamped in the clamping groove and rotates along the first circumference when the clamping rod is clamped in the clamping groove, the clamping rod is located in the fan-shaped groove, and the four inner walls of the fan-shaped groove are in contact with the clamping rod and play a role in limiting.
Preferably, the two magnetic bead transfer mechanisms and the two sampling bottles are arranged, the chip is provided with two sample adding ports, one sampling bottle, the two sample adding ports and the other sampling bottle are sequentially arranged along the second straight line, and the two magnetic bead transfer mechanisms are respectively used for transferring the magnetic beads in the two sampling bottles to the two sample adding ports;
and a detector is fixedly arranged right above the centrifugal station and connected with the human-computer interaction terminal, and the detector is used for detecting a detection result in the chip and directly transmitting the detection result to the human-computer interaction terminal.
Compared with the prior art, the invention has the following technical effects:
the nucleic acid elution and detection device provided by the invention can elute the magnetic beads which adsorb air microorganism nucleic acid in the sampling bottle and detect the eluted nucleic acid; utilize this device can realize the semi-automatic operation of air microorganism detection, only need to put into this device with the sample of gathering and the required chip of detection, can accomplish the detection to air microorganism sample, and easy operation, required instrument and equipment are few, and technical requirement is low, and simultaneously, this device simple structure, and low in production cost, it is convenient quick more to make air microorganism detection, easily popularization, in addition, except air microorganism sample, this device also can be used to elute and detect the nucleic acid sample of this elution to other magnetic beads that are in the binding solution and adsorb that have nucleic acid sample, save the manual operation of nucleic acid elution and detection.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required in the embodiments will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a nucleic acid eluting and detecting device according to the present invention;
FIG. 2 is a schematic structural view of a nucleic acid eluting and detecting device provided with a housing according to the present invention;
FIG. 3 is a schematic view of the nucleic acid eluting and detecting device with a sample bottle and a sleeve;
FIG. 4 is a schematic view of the structure of the heater plate separated from the chip;
FIG. 5 is a schematic view of the structure of the heater plate in contact with the die;
FIG. 6 is an exploded view of the heating mechanism;
FIG. 7 is a schematic diagram of the construction of the base and the centrifugal mechanism;
FIG. 8 is a schematic diagram of a chip;
FIG. 9 is a schematic view of a fixing rod;
FIG. 10 is a schematic structural view of a bushing;
in the figure: 1. a magnetic bead transfer mechanism; 11. a horizontal linear drive mechanism; 12. a lifting drive mechanism; 2. a fixing rod; 21. a transverse chute; 22. a vertical chute; 23. a magnet; 3. a sampling bottle fixing seat; 31. a sampling bottle 4 and a centrifugal mechanism; 41. a rotating shaft; 42. a clamping rod; 5. a heating mechanism; 51. heating plates; 52. a spring; 53. a fixed block; 54. a second electromagnet; 55. a first electromagnet; 6. a housing; 7. a base; 8. a chip; 81. a reaction chamber; 82. a sample addition port; 83. a card slot; 84. a sector groove; 9. a sleeve; 91. a slider; 92. a magnet area.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention aims to provide a nucleic acid elution and detection device, which aims to solve the problems in the prior art.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
The invention provides a nucleic acid elution and detection device, as shown in figures 1-10, comprising:
the upper end of the sampling bottle 31 is open, the sampling bottle 31 is used for containing binding liquid, a sample and magnetic beads, and nucleic acid of the sample can be adsorbed by the magnetic beads in the binding liquid;
the centrifugal mechanism 4 is provided with a centrifugal station, the centrifugal station is used for arranging a chip 8, and the centrifugal mechanism 4 is used for centrifuging the chip 8 on the centrifugal station;
the magnetic bead transferring mechanism 1 is used for transferring the magnetic beads with nucleic acids adsorbed in the sampling bottle 31 to a sample adding port 82 of the chip 8 on the centrifugal station, and eluting the nucleic acids on the magnetic beads at the sample adding port 82;
The nucleic acid elution and detection device provided by the invention can elute the magnetic beads which adsorb the air microorganism nucleic acid in the sampling bottle 31 and detect the eluted nucleic acid; utilize this device can realize the semi-automatization operation that air microorganism detected, only need to put into this device with the sample of gathering and the required chip 8 of detection, can accomplish the detection to air microorganism sample, easy operation, required instrument and equipment is few, and technical requirement is low, simultaneously, this device simple structure, low in production cost makes air microorganism detect convenient more quick, easy popularization.
Furthermore, the binding solution is a lysis binding solution, and the lysis binding solution is used for lysing the microorganism sample in the sampling bottle 31 and releasing nucleic acid in the sampling process, so that the magnetic beads adsorb the nucleic acid bound to the microorganism sample in the sampling process, the effect of extracting nucleic acid while sampling is achieved, and the efficiency is improved.
Furthermore, the nucleic acid eluting and detecting device also comprises a control unit, and the control unit controls the centrifugal mechanism 4, the magnetic bead transferring mechanism 1 and the heating mechanism 5 to work in sequence.
Further, the heating mechanism 5 includes a heating plate 51, a spring 52, a first electromagnet 55, and a second electromagnet 54; the heating plate 51 is arranged on one side of the chip 8 on the centrifugal station, the spring 52 is positioned on one side of the heating plate 51 far away from the chip 8, one end of the spring 52 is fixedly connected with the heating plate 51, the other end of the spring is fixedly arranged, the first electromagnet 55 is fixedly arranged on the surface of one side of the heating plate 51, which is provided with the spring 52, the second electromagnet 54 is fixedly arranged on one side of the first electromagnet 55 far away from the heating plate 51, and a gap is arranged between the second electromagnet 54 and the first electromagnet 55; when the second electromagnet 54 and the first electromagnet 55 are not energized, the heating plate 51 is in contact with the chip 8 under the elastic force of the spring 52; when the second electromagnet 54 and the first electromagnet 55 are energized, the second electromagnet 54 and the first electromagnet 55 attract each other and the attraction force can overcome the elastic force of the spring 52 to attract the heating plate 51 to be separated from the chip 8; when the second electromagnet 54 and the first electromagnet 55 are electrified, the second electromagnet 54 and the first electromagnet attract each other, compress the spring 52 and are combined together, so that the heating plate 51 is at a lower position, and the heating plate 51 is not in direct contact with the chip 8 on the centrifugal station; when the second electromagnet 54 and the first electromagnet 55 are powered off, the second electromagnet 54 and the first electromagnet 55 lose magnetism, and the heating plate 51 can be lifted to the position below the chip 8 and contacted with the chip 8 under the action of the elastic force of the spring 52, at the moment, the heating plate 51 is positioned below the reaction chamber 81 of the chip 8, and the reaction chamber 81 of the chip 8 can be heated after the heating plate 51 is powered on; through the above structural arrangement, the centrifugation and heating operations of the chip 8 are not interfered with each other.
Further, heating mechanism 5 still includes base 7, and hot plate 51 sets up the below of the chip 8 on the centrifugal station, and base 7 is fixed to be set up in hot plate 51's below, and the spring 52 is kept away from the one end fixed setting of hot plate 51 on base 7, and second electro-magnet 54 is also fixed in on the base 7.
Further, the heating device comprises a chip 8, the heating plate 51 is annular, the heating mechanism 5 comprises a plurality of springs 52, a plurality of first electromagnets 55 and a plurality of second electromagnets 54, the plurality of springs 52 are uniformly distributed on one side of the heating plate 51 along the circumferential direction of the heating plate 51, the plurality of first electromagnets 55 are uniformly distributed on one side of the heating plate 51 along the circumferential direction of the heating plate 51, the second electromagnets 54 and the first electromagnets 55 are arranged in a one-to-one correspondence manner, a plurality of reaction chambers 81 are arranged in the chip 8, orthographic projections of the plurality of reaction chambers 81 of the chip 8 positioned on a centrifugal station on a horizontal plane are first projections, orthographic projections of the heating plate 51 on the horizontal plane are second projections, and the first projections are completely positioned in the second projections; the heating plate 51 is used for heating the reaction liquid in the reaction chamber 81, compared with the solid heating plate 51, the total area of the annular heating plate 51 adopted by the invention is smaller, the power required by the heating mechanism 5 is reduced by adopting the hollow-out annular heating plate 51 under the same heating temperature, and the heating efficiency is not reduced because the heating plate 51 is arranged below the reaction chamber 81 for heating, therefore, the structure is used for rapidly heating the heating chamber and reducing the use power of the heating mechanism 5, and the traditional energy-saving and environment-friendly concept is met.
Further, the number of the first electromagnets 55 is equal to the number of the second electromagnets 54 and the springs 52, and one first electromagnet 55 and one second electromagnet 54 are disposed in each spring 52, so that the direction of the attraction force of the first electromagnet 55 and the second electromagnet 54 in each spring 52 is the same as the direction of the elastic force of the spring 52, thereby enhancing the stability of the movement of the heating plate 51.
Further, still include sampling bottle fixing base 3, sampling bottle 31 is placed on sampling bottle fixing base 3.
Further, the magnetic bead transferring mechanism 1 comprises a lifting driving mechanism 12, a horizontal linear driving mechanism 11, a fixing rod 2 and a sleeve 9, the sleeve 9 is a disposable article, so as to avoid cross contamination between detections, the lifting driving mechanism 12 is fixedly arranged, the horizontal linear driving mechanism 11 is fixedly arranged at the lifting driving end of the lifting driving mechanism 12, the fixing rod 2 is vertically arranged, the top end of the fixing rod 2 is fixedly arranged at the horizontal driving end of the horizontal linear driving mechanism 11, a magnet 23 for adsorbing magnetic beads is fixedly arranged at the bottom end of the fixing rod 2, one end of the sleeve 9 is open, the other end of the sleeve 9 is closed, the sleeve 9 can be sleeved on the fixing rod 2 from the bottom end of the fixing rod 2, the magnet 23 can adsorb the magnetic beads through the sleeve 9, the part of the sleeve 9 corresponding to the magnet 23 is a magnet area 92, and the magnet area 92 is located at one end of the sleeve 9 far away from the opening, the horizontal linear driving mechanism 11 can drive the fixing rod 2 to move along the first straight line, the sampling bottle 31, the sample adding port 82 of the chip 8 is arranged along the second straight line, the first straight line is parallel to the second straight line, the arrangement direction of the sample adding ports 82 of the sampling bottle 31 and the chip 8 is matched with the extension direction of the guide rail of the horizontal linear driving mechanism 11, the lifting driving mechanism 12 is used for driving the sleeve 9 to move up and down so as to realize the adsorption, transfer and elution processes of magnetic beads, the specific elution process is as follows, when the horizontal linear driving mechanism 11 drives the sleeve 9 to move right above the sample adding port 82, the horizontal driving is stopped, the lifting driving mechanism 12, namely the driving sleeve 9, descends to the sample adding port 82 and drives the sleeve 9 to move up and down repeatedly at the sample adding port 82, a reaction liquid is added in advance in the sample adding port 82 of the chip 8, the sleeve 9 moving up and down accelerates the oscillation of the reaction liquid, the nucleic acid on the magnetic bead of sleeve pipe 9 bottom can be eluted to the reaction solution of shaking, consequently, the adsorption, the transfer and the elution process of magnetic bead have been realized to above-mentioned structure, and simple structure, the good reliability.
Further, the outer wall of the fixing rod 2 is provided with a vertical sliding groove 22 and a horizontal sliding groove 21, the vertical sliding groove 22 extends from the bottom end of the fixing rod 2 to the top end of the fixing rod 2 along the fixing rod 2, one end of the vertical sliding groove 22 close to the top end of the fixing rod 2 is a tail end, one end of the horizontal sliding groove 21 is communicated with the tail end of the vertical sliding groove 22, and the horizontal sliding groove 21 extends from the tail end of the vertical sliding groove 22 along the circumferential direction of the fixing rod 2; the inner wall of one end, provided with an opening, of the sleeve 9 is fixedly provided with a sliding block 91 matched with the widths of the vertical sliding groove 22 and the horizontal sliding groove 21, when the sleeve 9 is sleeved upwards from the bottom end of the fixing rod 2, the sliding block 91 is clamped in the vertical sliding groove 22 and moves towards the tail end of the vertical sliding groove 22, after the sliding block 91 moves to the tail end of the vertical sliding groove 22, the sliding block 91 can slide into the horizontal sliding groove 21 by rotating the sleeve 9 along the extending direction of the horizontal sliding groove 21, preferably, the outer wall of the fixing rod 2 is provided with two vertical sliding grooves 22 and two horizontal sliding grooves 21, one vertical sliding groove 22 and one horizontal sliding groove 21 are correspondingly arranged, the other vertical sliding groove 22 and the other horizontal sliding groove 21 are correspondingly arranged, and the two vertical sliding grooves 22 are uniformly distributed along the circumferential direction of the fixing rod 2; be provided with two sliders 91 on the inner wall of sleeve pipe 9, two sliders 91 correspond the setting with two vertical spouts 22 respectively, and above-mentioned structure has realized installing sleeve pipe 9 fast and has dismantled the purpose of sleeve pipe 9.
Further, the bottom surface of chip 8 is provided with a plurality of draw-in grooves 83 radially extending from the bottom surface center of chip 8, centrifugal mechanism 4 includes centrifugal drive device, pivot 41 and a plurality of kelly 42, the vertical setting of pivot 41, centrifugal drive device can drive pivot 41 rotatory, kelly 42 one end is fixed to be set up in pivot 41, the other end just extends to the direction of keeping away from pivot 41 along the direction of perpendicular to pivot 41 axis, a kelly 42 is corresponding to a draw-in groove 83, each kelly 42 homoenergetic joint is in the draw-in groove 83 corresponding with this kelly 42, so that set up chip 8 on centrifugal drive device, and pivot 41 passes through kelly 42 and drives chip 8 and rotate.
Further, offer the fan-shaped groove 84 that extends along first circumference on the lateral wall of draw-in groove 83, the centre of a circle of first circumference is located the central line of chip 8, when kelly 42 joint is in draw-in groove 83, rotate chip 8 along first circumference and can make kelly 42 be in fan-shaped groove 84, and four inner walls of fan-shaped groove 84 all contact with kelly 42 and play limiting displacement, above-mentioned structure can prevent that chip 8 from droing from kelly 42 when being centrifugal motion, when pivot 41 rotates, make chip 8 rotate through the wall that promotes fan-shaped groove 84 innermost.
Furthermore, two magnetic bead transfer mechanisms 1 and two sampling bottles 31 are arranged, two sampling ports 82 are arranged on the chip 8, one sampling bottle 31, the two sampling ports 82 and the other sampling bottle 31 are sequentially arranged along a second straight line, and the two magnetic bead transfer mechanisms 1 are respectively used for transferring magnetic beads in the two sampling bottles 31 to the two sampling ports 82 and can be used for simultaneously detecting two samples to be detected;
the fixed detector that is provided with directly over centrifugal station, the detector links to each other with human-computer interaction terminal, and the detector is used for detecting the testing result in the chip 8 and can directly transmit the testing result to human-computer interaction terminal, and the chip 8 structure of reaction chamber 81 top is made for transparent material to the reaction result of detector in to reaction chamber 81 detects.
Further, the solar water heater also comprises a shell 6, preferably in a cuboid or square shape, one side of the shell 6 is open, and the shell 6 comprises a top plate, a bottom plate and three side plates; sampling bottle 31, centrifugal mechanism 4, magnetic bead transfer mechanism 1 and heating mechanism 5 all set up in shell 6, and the staff puts into chip 8, sampling bottle 31 and sleeve pipe 9 on each subassembly through the side opening of shell 6.
The specific working flow of the device is as follows:
1. placing the two sampling bottles 31 into the two sampling bottle fixing seats 3 respectively, sleeving the sleeve 9 on the fixing rod 2, fixing the chip 8 on a centrifugal station, and adding a reaction solution into the sample adding port 82 of the chip 8;
wherein, the sample in the sampling bottle 31 is a sample obtained by taking the lysis binding solution as a sampling solution, adding the nucleic acid extraction magnetic beads into the sampling solution and collecting the air microorganisms.
2. The fixing rod 2 drives the sleeve 9 to move into the sampling bottle 31, and magnetic beads with nucleic acid adsorbed in the sampling bottle 31 are adsorbed to the outer side of a magnet area 92 of the sleeve 9;
3. the fixing rod 2 drives the sleeve 9 to move out of the sampling bottle 31 and move to the sample adding port 82 of the chip 8 on the centrifugal station, the fixing rod 2 drives the sleeve 9 to move up and down in a small amplitude to and fro, so that the magnet region 92 of the sleeve 9 repeatedly enters and exits in the reaction liquid of the sample adding port 82, and the nucleic acid adsorbed by the magnetic beads is eluted into the reaction liquid (the default positions of the centers of the two sample adding ports 82 of the chip 8 are on the same surface with the centers of the bases of the two sampling bottles 31 and the centers of the magnets on the two fixing rods 2);
4. the fixing rod 2 drives the sleeve 9 to move to the position above the sampling bottle 31 (the position is the default position), and the centrifugal mechanism 4 starts centrifugation to enable the solution of the chip sample adding port 82 to enter the reaction chamber 81;
5. the electromagnet of the heating structure is powered off (in a default state, the electromagnet of the heating structure is in a power-on state, the heating plate 51 is not in contact with the chip 8 and is in a closed state), and the heating plate 51 is in contact with the lower bottom surface of the chip 8;
6. the heating plate 51 is started to heat, so that the chip 8 performs detection reaction, after the reaction is finished, the heating plate 51 stops heating, the electromagnet is electrified, and the heating plate 51 descends;
7. and taking the chip 8 out of the device and observing the detection result or automatically photographing the detection result through the detector and transmitting the picture to the man-machine interaction terminal, so that an experimenter can directly check the detection result through the terminal.
The principle and the implementation mode of the invention are explained by applying a specific example, and the description of the embodiment is only used for helping to understand the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In view of the above, the present disclosure should not be construed as limiting the invention.
Claims (10)
1. A nucleic acid eluting and detecting device, characterized in that: the method comprises the following steps:
the sampling bottle is open at the upper end and is used for containing a binding solution, a sample and magnetic beads, and nucleic acid of the sample can be adsorbed by the magnetic beads in the binding solution;
the centrifugal mechanism is provided with a centrifugal station, the centrifugal station is used for arranging a chip, and the centrifugal mechanism is used for centrifuging the chip on the centrifugal station;
the magnetic bead transferring mechanism is used for transferring the magnetic beads, which are adsorbed with the nucleic acids in the sampling bottle, to a sample adding opening of the chip on the centrifugal station and eluting the nucleic acids on the magnetic beads at the sample adding opening;
a heating mechanism, a heating end of the heating mechanism being capable of contacting and separating from the chip.
2. The nucleic acid eluting and detecting device according to claim 1, wherein: the heating mechanism comprises a heating plate, a spring, a first electromagnet and a second electromagnet; the heating plate is arranged on one side of the chip on the centrifugal station, the spring is positioned on one side of the heating plate away from the chip, one end of the spring is fixedly connected with the heating plate, the other end of the spring is fixedly arranged, the first electromagnet is fixedly arranged on the surface of one side of the heating plate, which is provided with the spring, the second electromagnet is fixedly arranged on one side of the first electromagnet away from the heating plate, and a gap is formed between the second electromagnet and the first electromagnet;
when the second electromagnet and the first electromagnet are not electrified, the heating plate is contacted with the chip under the action of the elastic force of the spring; and under the state that the second electromagnet and the first electromagnet are electrified, the second electromagnet and the first electromagnet attract each other and the attraction can overcome the elasticity of the spring to attract the heating plate to be separated from the chip.
3. The nucleic acid eluting and detecting device according to claim 2, wherein: the heating mechanism further comprises a base, the heating plate is arranged below the chip on the centrifugal station, the base is fixedly arranged below the heating plate, one end, far away from the heating plate, of the spring is fixedly arranged on the base, and the second electromagnet is also fixed on the base.
4. The nucleic acid eluting and detecting device according to claim 3, wherein: the heating mechanism comprises a plurality of springs, a plurality of first electromagnets and a plurality of second electromagnets, the springs are uniformly distributed on one side of the heating plate along the circumferential direction of the heating plate, the first electromagnets are uniformly distributed on one side of the heating plate along the circumferential direction of the heating plate, the second electromagnets and the first electromagnets are arranged in a one-to-one correspondence manner, a plurality of reaction chambers are arranged in the chip, orthographic projections of the reaction chambers of the chip on the centrifugal station on a horizontal plane are first projections, orthographic projections of the heating plate on the horizontal plane are second projections, and the first projections are completely positioned in the second projections;
the number of the first electromagnets is equal to that of the second electromagnets and that of the springs, and one first electromagnet and one second electromagnet are arranged in each spring.
5. The nucleic acid eluting and detecting device according to claim 1, wherein: the magnetic bead transferring mechanism comprises a lifting driving mechanism, a horizontal linear driving mechanism, a fixed rod and a sleeve, the sleeve is a disposable article, the lifting driving mechanism is fixedly arranged, the horizontal linear driving mechanism is fixedly arranged at the lifting driving end of the lifting driving mechanism, the fixed rod is vertically arranged, the top end of the fixed rod is fixedly arranged at the horizontal driving end of the horizontal linear driving mechanism, the bottom end of the fixed rod is fixedly provided with a magnet for adsorbing magnetic beads, one end of the sleeve is open, the other end of the sleeve is closed, the sleeve can be sleeved on the fixed rod from the bottom end of the fixed rod, the magnet can adsorb the magnetic beads through the sleeve, the horizontal linear driving mechanism can drive the fixed rod to move along a first straight line, the sampling bottles and the sample adding ports of the chips are arranged along a second straight line, the first line and the second line are parallel.
6. The nucleic acid eluting and detecting device according to claim 5, wherein: the outer wall of the fixed rod is provided with a vertical sliding groove and a transverse sliding groove, the vertical sliding groove extends from the bottom end of the fixed rod to the top end of the fixed rod along the fixed rod, one end, close to the top end of the fixed rod, of the vertical sliding groove is a tail end, one end of the transverse sliding groove is communicated with the tail end of the vertical sliding groove, and the transverse sliding groove extends from the tail end of the vertical sliding groove along the circumferential direction of the fixed rod; the utility model discloses a slide assembly, including the casing pipe, the casing pipe is provided with the open-ended one end on the inner wall fixed be provided with the vertical spout with the slider of the width looks adaptation of horizontal spout, the casing pipe is followed when the bottom of dead lever is upwards established to the cover, the slider joint in the vertical spout and to the terminal of vertical spout removes, the slider removes to after the terminal of vertical spout, along the direction that horizontal spout extends rotates the casing pipe can make the slider slide to in the horizontal spout.
7. The nucleic acid eluting and detecting device according to claim 6, wherein: the outer wall of the fixed rod is provided with two vertical sliding grooves and two transverse sliding grooves, one vertical sliding groove and one transverse sliding groove are correspondingly arranged, the other vertical sliding groove and the other transverse sliding groove are correspondingly arranged, and the two vertical sliding grooves are uniformly distributed along the circumferential direction of the fixed rod; two sliding blocks are arranged on the inner wall of the sleeve, and the two sliding blocks are respectively arranged corresponding to the two vertical sliding grooves.
8. The nucleic acid eluting and detecting device according to claim 1, wherein: the bottom surface of chip is provided with certainly the bottom surface center of chip along a plurality of draw-in grooves of bottom surface radial extension, centrifugal mechanism includes centrifugal drive device, pivot and a plurality of kelly, the vertical setting of pivot, centrifugal drive device can drive the pivot is rotatory, kelly one end is fixed set up in the pivot, the other end along the perpendicular to the direction of pivot axis just to keeping away from the direction of pivot extends, one the kelly is corresponding to one the draw-in groove, each the kelly homoenergetic joint in with this the kelly is corresponding in the draw-in groove.
9. The nucleic acid eluting and detecting device according to claim 8, wherein: the side wall of the clamping groove is provided with a fan-shaped groove extending along a first circumference, the circle center of the first circumference is located on the central line of the chip, the clamping rod is clamped in the clamping groove and rotates along the first circumference when the clamping rod is in the clamping groove, the clamping rod can be located in the fan-shaped groove, and four inner walls of the fan-shaped groove are in contact with the clamping rod and play a limiting role.
10. The nucleic acid eluting and detecting device according to claim 6, wherein: the two magnetic bead transferring mechanisms and the two sampling bottles are arranged, the chip is provided with two sample adding ports, one sampling bottle, the two sample adding ports and the other sampling bottle are sequentially arranged along the second straight line, and the two magnetic bead transferring mechanisms are respectively used for transferring the magnetic beads in the two sampling bottles to the two sample adding ports;
and a detector is fixedly arranged right above the centrifugal station and connected with the human-computer interaction terminal, and the detector is used for detecting a detection result in the chip and directly transmitting the detection result to the human-computer interaction terminal.
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| CN202210649205.7A CN114736787A (en) | 2022-06-10 | 2022-06-10 | Nucleic acid elution and detection device |
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| CN202210649205.7A CN114736787A (en) | 2022-06-10 | 2022-06-10 | Nucleic acid elution and detection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115161188A (en) * | 2022-09-07 | 2022-10-11 | 至美时代生物智能科技(北京)有限公司 | Air microorganism detection system and method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103403521A (en) * | 2011-03-08 | 2013-11-20 | 拉瓦勒大学 | Fluidic centripetal device |
| CN104204229A (en) * | 2012-02-10 | 2014-12-10 | 株式会社百奥尼 | Apparatus and method for automatically analyzing biological samples |
| CN111909841A (en) * | 2019-05-08 | 2020-11-10 | 中科欧蒙未一(北京)医学技术有限公司 | Variable-temperature centrifugal device |
| CN113201529A (en) * | 2021-04-30 | 2021-08-03 | 杭州米欧仪器有限公司 | Nucleic acid extraction method |
| CN114364811A (en) * | 2019-09-20 | 2022-04-15 | 美国西门子医学诊断股份有限公司 | Sample preparation apparatus with PCR chip and multi-well plate |
-
2022
- 2022-06-10 CN CN202210649205.7A patent/CN114736787A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103403521A (en) * | 2011-03-08 | 2013-11-20 | 拉瓦勒大学 | Fluidic centripetal device |
| CN104204229A (en) * | 2012-02-10 | 2014-12-10 | 株式会社百奥尼 | Apparatus and method for automatically analyzing biological samples |
| CN111909841A (en) * | 2019-05-08 | 2020-11-10 | 中科欧蒙未一(北京)医学技术有限公司 | Variable-temperature centrifugal device |
| CN114364811A (en) * | 2019-09-20 | 2022-04-15 | 美国西门子医学诊断股份有限公司 | Sample preparation apparatus with PCR chip and multi-well plate |
| CN113201529A (en) * | 2021-04-30 | 2021-08-03 | 杭州米欧仪器有限公司 | Nucleic acid extraction method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115161188A (en) * | 2022-09-07 | 2022-10-11 | 至美时代生物智能科技(北京)有限公司 | Air microorganism detection system and method |
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Application publication date: 20220712 |