CN114712567A - Degradable biological membrane for guiding bone regeneration and preparation method and application thereof - Google Patents
Degradable biological membrane for guiding bone regeneration and preparation method and application thereof Download PDFInfo
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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- A61C8/00—Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereon; Dental implants; Implanting tools
- A61C8/0012—Means to be fixed to the jaw-bone for consolidating natural teeth or for fixing dental prostheses thereon; Dental implants; Implanting tools characterised by the material or composition, e.g. ceramics, surface layer, metal alloy
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
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- A—HUMAN NECESSITIES
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
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- A61F2002/2835—Bone graft implants for filling a bony defect or an endoprosthesis cavity, e.g. by synthetic material or biological material
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
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- A—HUMAN NECESSITIES
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/12—Materials or treatment for tissue regeneration for dental implants or prostheses
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Abstract
The invention discloses a degradable guided bone regeneration biomembrane and a preparation method and application thereof, relates to the field of orthopedic treatment, and aims to solve the problem that the existing orthopedic treatment needs to be performed with two or even multiple times of excision and operation operations. The following scheme is proposed, which includes: biomembrane skeleton, biomembrane and promoter, the biomembrane skeleton is made by absorbable metal material, the biomembrane is made by freeze-drying xenogeneic periosteum and polyhydroxybutyrate mixture, the biomembrane skeleton set up in the biomembrane is inside and extend biomembrane one end, the biomembrane skeleton extends the position and is used for fixing and installing. The degradable metal framework is arranged to support the biological membrane, the growth promoter is filled between the degradable metal framework and the biological membrane, the metal framework is selected according to the condition of the patient and the condition of elements receivable by the body, the metal framework can be degraded and absorbed in the body of the patient, and the biological membrane is combined with the growth promoter, so that the physicochemical property and the biocompatibility of the biological membrane are improved.
Description
Technical Field
The invention relates to the field of orthopedic treatment, in particular to a degradable guided bone regeneration biomembrane and a preparation method and application thereof.
Background
Osteomyelitis is the continuation of acute suppurative osteomyelitis, usually the general symptoms mostly disappear, only when local drainage is not smooth, the general symptoms are limited to the local part, and are usually stubborn and difficult to treat, even the general symptoms can not be cured for years or decades, when the general symptoms enter the disease period of chronic osteomyelitis clinically, local swelling, sclerotin thickening, surface roughness and tenderness exist, if sinus tracts exist, wounds can not be cured for a long time, small dead bones are occasionally discharged, sometimes the wounds can be temporarily cured, but due to infection focuses and inflammation diffusion, acute attack can be caused; the whole body is cold and hot, the local part is red and swollen, and the wound is cut for drainage or is punctured by the self; at present, general symptoms disappear and local inflammation gradually subsides after drug control, but the symptoms are easy to recur, the drug treatment is difficult to cure, and the infected bone part needs to be cut off when the operation treatment is carried out, so that the operation treatment is usually carried out for a plurality of times for a long time, the time is long, and the operation difficulty is increased.
In the treatment of human orthopedics field, the healing of bone tissues is usually promoted, the repair and regeneration of bone defects are promoted, for example, the repair and treatment of bone defects of osteomyelitis patients, accidents or other conditions, for example, pathological fracture, the treatment and operation of limb shortening or angulation deformity are required, the operation is usually performed by cutting off the affected part of the patient, bone marrow mud is adopted for repair after occupying space, the affected part is opened again after recovering for 1-2 months, bone is implanted for recovery after bone marrow mud is taken out, a fixing frame is required for fixing, the removal and operation are required for two or even more times, risks are caused, and the treatment period is long, so that the invention is necessary for the degradable guided bone regeneration biomembrane and the preparation method and the application thereof.
Disclosure of Invention
Objects of the invention
In view of the above, the present invention aims to provide a degradable guided bone regeneration biofilm, a preparation method and an application thereof, so as to improve the overall supporting strength of the biofilm, ensure that a metal framework can be degraded and absorbed in a pathological part of a patient, and improve the physicochemical property and the biocompatibility of the biofilm by combining the biofilm with a growth promoter, so that the biofilm has the transferring and inducing capabilities.
(II) technical scheme
In order to achieve the above technical objects, the present invention provides a degradable guided bone regeneration biofilm, comprising: biomembrane skeleton, biomembrane and promoter, the biomembrane skeleton is made by absorbable metal material, the biomembrane is made by freeze-drying xenogeneic periosteum and polyhydroxybutyrate mixture, the biomembrane skeleton set up in the biomembrane is inside and extend biomembrane one end, the biomembrane skeleton extends the position and is used for fixing and installing.
Preferably, the anabolic agent comprises a bone morphogenetic protein and an inducing agent.
The specific biological membrane is combined with the growth-promoting agent, so that the physical and chemical properties of the biological membrane are improved, the biocompatibility of the biological membrane is improved, and the biological membrane has the transferring and inducing capabilities, such as guiding the regeneration of defective bone tissues after being compounded with bone morphogenetic proteins and the biological membrane, and improving the effect of the biological membrane.
Preferably, the biological membrane skeleton comprises a magnesium skeleton, a sodium hyaluronate skeleton, a titanium mesh skeleton or a polypropylene skeleton.
The metal framework is selected according to the disease state of the patient and the receivable element condition of the body, so that the metal framework can be degraded and absorbed in the body of the patient.
Preferably, the biofilm skeleton is shaped like a net.
The specific reticular biomembrane framework can ensure the overall stability and cannot be easily deformed.
A preparation method of a degradable biological membrane for guiding bone regeneration comprises the following preparation steps:
the method comprises the following steps: selecting raw materials of a biomembrane framework and a biomembrane, and storing the two raw materials in a classified manner;
step two: processing and processing raw materials, sequentially adding the raw materials of the biomembrane and the raw materials of the biomembrane framework into different organic solvents, fully stirring and dissolving the raw materials by stirring equipment, and gradually adding the loaded antibiotics in the stirring process to form a mixed solution;
step three: adding the mixed solution into a mold, heating and drying, volatilizing water in the mixed solution to remove the organic solvent, and forming a biological membrane;
step four: adding the mixed liquid of the biological membrane skeleton into a mold, limiting the position of the mixed liquid in the mold to ensure that the mixed liquid forms a net shape after being molded, and then heating and drying the mold to mold the biological membrane skeleton;
step five: and (3) installing the formed biomembrane framework in the biomembrane, and adding a growth promoting agent in the installation gap part to complete the whole preparation process of the degradable guided bone regeneration biomembrane.
Preferably, the preparation of the biofilm and the biofilm matrix needs to be adjusted and fine-tuned according to the size of the model required by the patient.
The metal framework can be degraded and absorbed in the body of a patient.
In the fourth step of the third step, the heating temperature is kept at 80-120 ℃, and the heating is continued for 16-20 h.
Specifically, the heating is carried out for a long time in a low temperature state, so that the performance reduction of the biomembrane and the biomembrane framework is avoided, and the water in the biomembrane framework is slowly volatilized.
The application of the degradable guided bone regeneration biomembrane is applied to the field of orthopedic treatment and the field of stomatological treatment.
Specifically, the degradable guided bone regeneration biomembrane is applied to the fields of orthopedics and stomatology, and can assist bone tissue healing and promote repair and regeneration of bone defects.
Preferably, the orthopedic treatment fields include chronic osteomyelitis treatment, bone defect treatment and orthopedic repair and correction, and the stomatological treatment fields include immediate implantation after tooth extraction, dental implantation of alveolar ridge bone mass, remedy treatment of tooth implantation failure and combined application of a membrane and a bone.
Specifically, the degradable guided bone regeneration biomembrane can be used for medical treatment in the field of dental implantation, can make up for respective technical defects, and is beneficial to wide development of clinical treatment.
According to the technical scheme, the method has the following beneficial effects:
1: the degradable metal framework is arranged as the biological membrane support, the growth promoter is filled between the degradable metal framework and the biological membrane support, the metal framework is selected according to the disease state of a patient and the condition of elements which can be received by the body of the patient, the metal framework can be degraded and absorbed in the body of the patient, the integral support strength of the biological membrane can be improved, the biological membrane is combined with the growth promoter, the physicochemical property and the biocompatibility of the biological membrane are improved, and the biological membrane has the transferring and inducing capability, for example, the bone morphogenetic protein and the biological membrane are compounded to guide the regeneration of defective bone tissues and improve the effect of the biological membrane;
2: the degradable guide bone regeneration biomembrane is applied to the field of orthopedic treatment and the field of dental implantation, a relatively closed space can be manufactured by utilizing the biomembrane for healing bone tissues, epithelial cells or connective tissues with higher growth speed are prevented from entering a bone defect area, and further the repair and regeneration of bone defects are facilitated, particularly the treatment of the part of osteomyelitis, the treatment and recovery of the orthopedic fields such as the repair and treatment of bone defects and the like are facilitated.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a flow chart of the preparation of a degradable guided bone regeneration biomembrane provided by the invention.
Fig. 2 is a flow chart for preparing a degradable guided bone regeneration biomembrane provided by the invention.
Detailed Description
The following description is merely exemplary in nature and is not intended to limit the present disclosure, application, or uses. It should be understood that throughout the drawings, identical or similar reference numerals indicate identical or similar parts and features. The drawings are only schematic representations of the concepts and principles of the embodiments of the disclosure, and do not necessarily show specific dimensions or proportions of the various embodiments of the disclosure. Certain features that are part of a particular figure may be exaggerated in order to illustrate relevant details or structures of embodiments of the present disclosure.
Referring to FIGS. 1-2:
example one
A degradable guided bone regeneration biofilm comprising: biomembrane skeleton, biomembrane and promoter, biomembrane skeleton are made by absorbable metal material, and the biomembrane is made by freeze-drying allogeneic periosteum and polyhydroxybutyrate mixture, and the biomembrane skeleton sets up in the biomembrane inside and extend biomembrane one end, and the biomembrane skeleton extends the position and is used for fixing and installing.
In this embodiment, the anabolic agent includes a bone morphogenetic protein and an inducing agent. The biomembrane is combined with the growth promoting agent to improve the physicochemical property and the biocompatibility of the biomembrane, so that the biomembrane has the transferring and inducing capability, and what needs to be explained is that the biomembrane is compounded with bone morphogenetic protein to guide the regeneration of defective bone tissues and improve the effect of the biomembrane.
In this embodiment, the biofilm skeleton includes a magnesium skeleton, a sodium hyaluronate skeleton, a titanium mesh skeleton, or a polypropylene skeleton. In particular, the metal framework is selected according to the self-pathology and the receivable element condition of the body of the patient, and the requirement to be explained is to ensure that the metal framework can be degraded and absorbed in the body of the patient.
In the present embodiment, the biofilm skeleton is formed in a net shape. The specific reticular biomembrane framework can ensure the overall stability of the biomembrane framework, and the requirement is that the biomembrane framework cannot be easily deformed.
Example two
A preparation method of a degradable biological membrane for guiding bone regeneration is based on the first embodiment and comprises the following preparation steps:
the method comprises the following steps: selecting raw materials of a biomembrane framework and a biomembrane, and storing the two raw materials in a classified manner;
step two: processing and processing raw materials, sequentially adding the raw materials of the biomembrane and the raw materials of the biomembrane framework into different organic solvents, fully stirring and dissolving the raw materials by stirring equipment, and gradually adding the loaded antibiotics in the stirring process to form a mixed solution;
step three: adding the mixed solution into a mold, heating and drying, volatilizing water in the mixed solution to remove the organic solvent, and forming a biological membrane;
step four: adding the mixed liquid of the biological membrane skeleton into a mold, limiting the position of the mixed liquid in the mold to ensure that the mixed liquid forms a net shape after being molded, and then heating and drying the mold to mold the biological membrane skeleton;
step five: and (3) installing the formed biomembrane framework in the biomembrane, and adding a growth promoting agent in the installation gap part to complete the whole preparation process of the degradable guided bone regeneration biomembrane.
In this embodiment, the preparation of the biofilm and biofilm matrix needs to be adjusted and fine-tuned according to the size of the model required by the patient. In particular to ensure that the metal framework can be degraded and absorbed in the body of a patient.
In the fourth step of the third step in the embodiment, the heating temperature is kept at 80-120 ℃, and the heating is continued for 16-20 h. Specifically, the heating is carried out for a long time in a low temperature state, so as to avoid the performance reduction of the biological membrane and the biological membrane skeleton, and what needs to be explained is that the water in the biological membrane is slowly volatilized.
EXAMPLE III
The application of the degradable guided bone regeneration biomembrane is based on the second embodiment, and the degradable guided bone regeneration biomembrane is applied to the field of orthopedic treatment and the field of stomatological treatment.
In this embodiment, the orthopedic treatment fields include chronic osteomyelitis treatment, bone defect treatment, and orthopedic repair and correction, and the stomatological treatment fields include immediate implantation after tooth extraction, dental implantation of alveolar ridge bone mass, remedy treatment of tooth implantation failure, and combined use of a membrane and a bone. Specifically, a relatively closed space can be manufactured by utilizing a biomembrane for healing bone tissues, epithelial cells or connective tissues with higher growth speed are prevented from entering a bone defect area, and further, the repair and regeneration of bone defects are facilitated, particularly, the treatment and the recovery of the treatment of osteomyelitis parts, the repair and the treatment of bone defects and other orthopedic fields are facilitated.
Exemplary embodiments of the proposed solution of the present disclosure have been described in detail above with reference to preferred embodiments, however, it will be understood by those skilled in the art that many variations and modifications may be made to the specific embodiments described above, and that many combinations of the various technical features and structures presented in the present disclosure may be made without departing from the concept of the present disclosure, without departing from the scope of the present disclosure, which is defined by the appended claims.
Claims (9)
1. A degradable guided bone regeneration biofilm, comprising: biomembrane skeleton, biomembrane and promoter, the biomembrane skeleton is made by absorbable metal material, the biomembrane is made by freeze-drying xenogeneic periosteum and polyhydroxybutyrate mixture, the biomembrane skeleton set up in the biomembrane is inside and extend biomembrane one end, the biomembrane skeleton extends the position and is used for fixing and installing.
2. The degradable guided bone regeneration biofilm according to claim 1, wherein said anabolic agents comprise bone morphogenic proteins and inducing agents.
3. The degradable guided bone regeneration biofilm according to claim 1, wherein the biofilm matrix comprises a magnesium matrix, a sodium hyaluronate matrix, a titanium mesh matrix or a polypropylene matrix.
4. The degradable guided bone regeneration biofilm according to claim 1, wherein said biofilm skeleton is configured in a mesh shape.
5. A method for preparing a degradable guided bone regeneration biofilm, which is used for preparing the biofilm according to any one of claims 1 to 4, and comprises the following preparation steps:
the method comprises the following steps: selecting raw materials of a biomembrane framework and a biomembrane, and storing the two raw materials in a classified manner;
step two: processing and processing raw materials, sequentially adding the raw materials of the biomembrane and the raw materials of the biomembrane framework into different organic solvents, fully stirring and dissolving the raw materials by stirring equipment, and gradually adding the loaded antibiotics in the stirring process to form a mixed solution;
step three: adding the mixed solution into a mold, heating and drying, volatilizing water in the mixed solution to remove the organic solvent, and forming a biological membrane;
step four: adding the mixed liquid of the biological membrane skeleton into a mold, limiting the position of the mixed liquid in the mold to ensure that the mixed liquid forms a net shape after being molded, and then heating and drying the mold to mold the biological membrane skeleton;
step five: and (3) installing the formed biomembrane framework in the biomembrane, and adding a growth promoting agent in the installation gap part to complete the whole preparation process of the degradable guided bone regeneration biomembrane.
6. The method for preparing a degradable guided bone regeneration biofilm according to claim 5, wherein the preparation of the biofilm and the biofilm skeleton is adjusted and fine-tuned according to the size of a patient.
7. The method for preparing a degradable guided bone regeneration biofilm according to claim 5, wherein in the fourth step of the third step, the heating temperature is kept between 80 ℃ and 120 ℃, and the heating is continued for 16 to 20 hours.
8. Use of a degradable guided bone regeneration biofilm according to any one of claims 1 to 4 in the field of orthopedic treatment and in the field of oral treatment.
9. The use of a degradable guided bone regeneration biofilm according to claim 8, wherein said orthopedic treatment fields include chronic osteomyelitis treatment, bone defect treatment and orthopedic repair and correction, and said oral treatment fields include immediate implantation after tooth extraction, dental implantation of alveolar ridge bone mass, remedy for tooth implantation failure and combined use of a film and a bone.
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