CN114651725B

CN114651725B - Orchid seedling group banks up device with earth based on intelligent regulation and control

Info

Publication number: CN114651725B
Application number: CN202210422981.3A
Authority: CN
Inventors: 蒋彧; 何俊蓉; 刘雅琴; 涂勋良
Original assignee: Horticulture Research Institute of Sichuan Academy of Agricultural Sciences
Current assignee: Horticulture Research Institute of Sichuan Academy of Agricultural Sciences
Priority date: 2022-04-21
Filing date: 2022-04-21
Publication date: 2023-01-10
Anticipated expiration: 2042-04-21
Also published as: CN114651725A

Abstract

The invention discloses an orchid seedling tissue culture device based on intelligent regulation and control, and relates to the technical field of orchid seedling tissue culture devices.

Description

Orchid seedling group banks up device with earth based on intelligent regulation and control

Technical Field

The invention relates to the technical field of orchid seedling tissue culture devices, in particular to an orchid seedling tissue culture device based on intelligent regulation and control.

Background

The tissue culture propagation of orchid seedlings is a process of obtaining a certain part of tissue of an orchid and carrying out subculture in an isolated state by adopting a tissue culture mode to generate a new plant, wherein the certain part of the tissue of the orchid is usually part of a root, a stem and a branch or leaves, but the traditional device has some defects, cannot adapt to different nutritional requirements of various tissues of the orchid, causes a narrow application range, and cannot continuously and efficiently generate the orchid seedlings with high quality by different tissues of the orchid in a tissue culture mode;

in view of the above technical drawbacks, a solution is proposed.

Disclosure of Invention

The invention aims to: on the basis of generating orchid seedlings through continuous tissue culture, efficient and high-quality tissue culture of the orchid seedlings is achieved, and micro-pressure control of micro-addition liquid, addition of the micro-addition liquid in quantitative control and selective control of the micro-addition liquid with different requirements and formulas are achieved through cooperation of the constant-pressure control assembly, the quantitative addition assembly and the switching driving assembly, so that the tissue culture device can be widely adapted to different nutritional requirements of tissue culture of various orchids, and the tissue culture range of the tissue culture device is wider;

in order to achieve the purpose, the invention adopts the following technical scheme:

the orchid seedling tissue culture device based on intelligent regulation comprises a tissue culture incubator, wherein a suspension centrifugal box and a tissue culture installation box are fixedly arranged in the tissue culture incubator, the suspension centrifugal box is arranged on the bottom wall of the tissue culture incubator, a centrifugal pushing assembly is arranged on the suspension centrifugal box, the centrifugal pushing assembly is arranged on the tissue culture incubator, a plurality of tissue culture installation boxes are arranged at equal intervals, a tissue culture screen plate is movably clamped on the top surface of the tissue culture installation box, a plurality of tissue placement openings used for placing orchid tissues are formed in the tissue culture screen plate, a seepage screen used for supporting the orchid tissues is arranged at the bottom end of each tissue placement opening, and the seepage screen is fixedly arranged at the bottom end of each tissue culture screen plate;

the utility model discloses a tissue culture device, including suspension centrifugation case, distributing liquid pump, honeycomb duct, constant voltage control assembly, switch drive assembly, constant voltage control assembly and micro-batch storage tube group, the top of suspension centrifugation case has the distributing liquid pump through connection through the pipeline, the inlet through connection of distributing liquid pump is in the top of suspension centrifugation case, the liquid outlet through connection of distributing liquid pump has the distributing pipe, the one end through connection that the distributing pipe keeps away from the distributing liquid pump is in the bottom center department of group's tissue culture install bin, the bottom both sides through connection of suspension centrifugation case has honeycomb duct and feed liquor pipe, install the feed liquor choke valve on the feed liquor pipe, the one end through connection of honeycomb duct has the drainage fill, the spacing case of top fixedly connected with spacing case of drainage fill, and spacing case and drainage fill through connection, spacing case is equipped with little interpolation control structure and micro-batch storage tube group, little interpolation control structure includes headstock, constant voltage control assembly, ration interpolation subassembly and switching drive assembly, the micro-batch storage tube group is fixed in the top of spacing case, little material storage tube group and is embedded in spacing incasement and rather than swing joint, little material storage tube group is equipped with a plurality ofly, and micro-batch storage tube group is central and is distributed according to the centre of circle of spacing case, and constant voltage control assembly adaptation setting with the constant voltage drive assembly.

Further, the centrifugation promotes the subassembly by centrifugal motor, centrifugation bull stick, centrifugation cover, centrifugal flabellum, promotion cover and catch bar and constitute, the fixed bottom of locating the group's culture of temperature cabinet of centrifugal motor, the centrifugation bull stick rotates and locates the suspension centrifugation incasement, and the one end of centrifugation bull stick runs through the inner wall of suspension centrifugation case and the inner wall of group's culture of temperature cabinet in proper order and extends to its outside and with the output shaft fixed connection of centrifugal motor, the outer end of centrifugation bull stick is equipped with centrifugation cover and promotion cover, and the fixed cover in outer end of centrifugation cover is equipped with centrifugal flabellum, the fixed cover in outer end of promotion cover is equipped with the catch bar, the top of centrifugal flabellum is located to the catch bar.

Further, the micro-material storage tube group comprises a liquid storage tube, a sliding block, a telescopic rod and an extrusion block, the sliding block is arranged in the liquid storage tube in a sliding mode, the top end of the sliding block is fixedly connected with the telescopic rod, the top end, far away from the sliding block, of the telescopic rod penetrates through the top wall of the liquid storage tube in a sliding mode and extends to the outside of the top wall and is fixedly connected with the extrusion block, and a liquid outlet is formed in the position, far away from the center, of the bottom end face of the liquid storage tube.

Further, the constant voltage control subassembly includes the lift panel, a plurality of clamping grooves have been seted up to the lift panel, the bottom outer loop side of lift panel is located to the clamping groove, and the clamping groove uses the centre of a circle of lift panel as the center and distributes according to annular array, the extrusion piece activity inlays and locates in the clamping groove, the lift panel cover is equipped with first spacing slide bar and screw thread bull stick, first spacing slide bar and lift panel sliding connection, first spacing slide bar is equipped with a plurality ofly, and just first spacing slide bar uses the centre of a circle of lift panel as the center and distributes according to annular array, the bottom of first spacing slide bar and the top face fixed connection of spacing case, screw thread bull stick and lift panel threaded connection, the top of screw thread bull stick and first spacing slide bar all is equipped with the anticreep piece, the bottom face and the lift panel activity butt of anticreep piece, and the bottom of screw thread bull stick rotate the outer wall that runs through spacing case and extend to its inside and fixed cover is equipped with first bevel gear, first bevel gear and switching drive subassembly activity meshing.

Further, the ration is added the subassembly and is included second bevel gear, second bevel gear and switching drive assembly active meshing, and the first bull stick of second bevel gear fixedly connected with, first bull stick rotates the setting, and first bull stick keeps away from the one end of second bevel gear and runs through the inside and fixed cover that the inner wall of headstock and the outer wall of spacing box extend to the spacing box in proper order and be equipped with first connecting sleeve pole, first connecting sleeve pole keeps away from the one end of first bull stick and installs micro-control motor, the fixed cover of output shaft of micro-control motor is equipped with the driving gear, driving gear active meshing is connected with quantitative gear, quantitative gear is equipped with a plurality ofly, and quantitative gear uses the axis of hollow bull stick to distribute as the centre of a circle and according to annular array, and quantitative gear and the setting of micro-material storage tube group one-to-for-axis, quantitative gear rotates the cover and is equipped with hollow bull stick, the fixed diapire of locating spacing case of hollow bull stick, the both ends butt of quantitative gear has spacing sealing wheel, spacing sealing wheel fixed cover is located the top of hollow bull stick is equipped with joint elastic sleeve, the outer end of joint elastic sleeve with the L of fixed sealing sleeve, the L is connected through-hole L, the L is seted up with the leakage hole of spacing arc-shaped leakage hole and the L is connected with the L of spacing box side.

Furthermore, the L-shaped leakage holes are arranged in a plurality of numbers, the L-shaped leakage holes are distributed in an annular array by taking the circle center of the quantitative gear as the center, the aperture of each L-shaped leakage hole is enlarged in an equal ratio, the maximum aperture of each L-shaped leakage hole is equal to the aperture of the liquid outlet, and the apertures of the liquid outlet and the side flow port are arranged in an equal size.

Further, the switching drive assembly comprises a lifting box, the lifting box is slidably arranged in the power box, a lifting air rod, a second connecting sleeve rod and a return spring are arranged on the lifting box, the lifting air rod is installed on the bottom wall of the power box, one end of the second connecting sleeve rod is fixedly connected with the power box, the other end of the second connecting sleeve rod is sleeved with a second limiting slide rod, the second limiting slide rod is fixedly arranged in the power box, the return spring is fixedly arranged at the bottom end of the power box, the other end of the return spring is fixedly arranged on the bottom wall of the power box, a servo motor is fixedly arranged in the power box, an output shaft of the servo motor is fixedly connected with a second rotating rod, the second rotating rod is rotatably arranged in the lifting box, one end of the second rotating rod penetrates through the inner wall of the lifting box and extends to the outside of the lifting box, a third bevel gear is fixedly sleeved on the other end of the return spring, the first bevel gear and the second bevel gear are arranged oppositely, and the symmetrical sides of the third bevel gear are respectively in clearance engagement with the first bevel gear and the second bevel gear.

Further, the base is installed to the bottom of group's thermostated container, a plurality of articulated arc doors are installed to the outer wall of group's thermostated container, every group banks up the installation box department with earth and all corresponds and set up an articulated arc door, install the door handle on the articulated arc door, control panel is installed to the outer end of group's thermostated container, last start button and the display screen of installing of control panel.

In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:

the tissue culture device realizes the high-efficiency and high-quality tissue culture of the orchid seedlings on the basis of the continuous tissue culture of the orchid seedlings, and realizes the micro-pressure control of the micro-addition liquid, the quantitative control of the addition of the micro-addition liquid and the selection control of the micro-addition liquid with different requirements and formulas through the cooperation of the constant-pressure control component, the quantitative addition component and the switching drive component, so that the tissue culture device can be widely adapted to different nutritional requirements of various tissues of the orchid, the tissue culture adaptation range of the tissue culture device is wider, the problems that the traditional device cannot be adapted to different nutritional requirements of various tissues of the orchid, the application range is narrow, and the orchid seedlings cannot be continuously generated with high efficiency and high quality through different tissues of the orchid in a tissue culture mode are solved.

Drawings

FIG. 1 shows a perspective view of the present invention;

FIG. 2 shows a schematic of the structure of the present invention;

FIG. 3 shows a schematic structural view of a centrifugal boost assembly;

FIG. 4 shows a top view of a tissue culture mesh plate;

FIG. 5 shows a schematic of the structure of a micro-addition control structure;

FIG. 6 shows a partial enlarged view at A of FIG. 5;

FIG. 7 shows a schematic of the structure at the dosing assembly;

fig. 8 shows a sectional view at the dosing gear;

illustration of the drawings: 1. tissue culture thermostat; 2. a suspension centrifuge box; 3. a centrifugal pushing assembly; 4. tissue culture installation box; 5. tissue culture net plate; 6. a flow guide hopper; 7. a limiting box; 8. a micro-addition control structure; 9. a micro-material storage tube group; 10. a dispensing pump; 11. a distribution pipe; 12. a flow guide pipe; 13. a liquid inlet pipe; 14. a liquid inlet throttle valve; 101. a base; 102. a control panel; 103. a start button; 104. a hinged arc door; 105. a door handle; 201. a centrifugal motor; 202. centrifuging the rotating rod; 203. a centrifugal sleeve; 204. centrifuging the fan blades; 205. a push sleeve; 206. a push rod; 501. a tissue placement port; 502. a liquid-permeable net; 801. a power box; 802. a constant voltage control assembly; 803. a quantitative addition component; 804. switching the driving assembly; 805. a lifting panel; 806. a first limit slide bar; 807. a threaded rotating rod; 808. a first bevel gear; 809. an anti-drop block; 810. an embedding groove; 811. a second bevel gear; 812. a first rotating lever; 813. a first connecting loop bar; 814. a micro-control motor; 815. a driving gear; 816. a dosing gear; 817. an L-shaped leak hole; 818. a limiting sealing wheel; 819. clamping the elastic sleeve; 820. fixing the arc sleeve; 821. a hollow rotating rod; 822. lifting the air rod; 823. a lifting box; 824. a servo motor; 825. a second rotating rod; 826. a third bevel gear; 827. a second connecting loop bar; 828. a second limit slide bar; 829. a return spring; 901. a liquid storage pipe; 902. a slider; 903. a telescopic rod; 904. extruding the block; 905. and a liquid outlet.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1:

as shown in fig. 1-8, the orchid seedling tissue culture device based on intelligent regulation comprises a tissue culture incubator 1, wherein a base 101 is installed at the bottom of the tissue culture incubator 1, a plurality of hinged arc doors 104 are installed on the outer wall of the tissue culture incubator 1, a hinged arc door 104 is correspondingly arranged at each 4 position of the tissue culture installation box, a door handle 105 is installed on each hinged arc door 104, a control panel 102 is installed at the outer end of the tissue culture incubator 1, and a starting button 103 and a display screen are installed on each control panel 102;

a suspension centrifugal box 2 and a tissue culture installation box 4 are fixedly arranged in the tissue culture incubator 1, the suspension centrifugal box 2 is arranged at the bottom wall of the tissue culture incubator 1, a centrifugal pushing assembly 3 is arranged on the suspension centrifugal box 2, the centrifugal pushing assemblies 3 are arranged on the tissue culture incubator 1, a plurality of tissue culture installation boxes 4 are arranged at equal intervals, a tissue culture screen plate 5 is movably clamped on the top surface of the tissue culture installation box 4, a plurality of tissue placement openings 501 for placing orchid tissues are formed in the tissue culture screen plate 5, a seepage net 502 for supporting the orchid tissues is arranged at the bottom end of each tissue placement opening 501, and the seepage net 502 is fixedly arranged at the bottom end of the tissue culture screen plate 5;

the top of the suspension centrifugal box 2 is connected with a distribution liquid pump 10 through a pipeline in a through way, the liquid inlet of the distribution liquid pump 10 is connected with the top of the suspension centrifugal box 2 through a through way, the liquid outlet of the distribution liquid pump 10 is connected with a distribution pipe 11 through a through way, one end of the distribution pipe 11 far away from the distribution liquid pump 10 is connected with the center of the bottom end of the tissue culture installation box 4 through a through way, two sides of the bottom of the suspension centrifugal box 2 are connected with a guide pipe 12 and a liquid inlet pipe 13 through a through way, a liquid inlet throttle valve 14 is installed on the liquid inlet pipe 13, one end of the guide pipe 12 is connected with a flow guide hopper 6 through a through way, the top end of the flow guide hopper 6 is fixedly connected with a limit box 7, and the limit box 7 is connected with the flow guide hopper 6 through a through way,

the limiting box 7 is provided with a micro-adding control structure 8 and a micro-material storage tube group 9, the micro-adding control structure 8 comprises a power box 801, a constant-pressure control component 802, a quantitative adding component 803 and a switching driving component 804, the power box 801 is fixedly arranged at the top end of the limiting box 7, the micro-material storage tube group 9 is embedded in the limiting box 7 and movably connected with the limiting box, a plurality of micro-material storage tube groups 9 are arranged, the micro-material storage tube groups 9 are distributed by taking the circle center of the limiting box 7 as the center and according to an annular array, different micro-adding liquids are stored in the micro-material storage tube group 9, the micro-material storage tube groups 9 are detachably arranged and can adapt to adjustment of more different culture liquids, the micro-material storage tube groups 9 and the quantitative adding component 803 are arranged in an adaptive mode, the constant-pressure control component 802 is abutted to the micro-material storage tube groups 9 in an adaptive mode, and the constant-pressure control component 802 and the quantitative adding component 803 are movably connected with the switching driving component 804 in a transmission mode;

the control panel 102 starts a centrifugal motor 201 fixedly installed at the bottom end of the tissue culture incubator 1 to work and controls an output shaft of the centrifugal motor to rotate, the output shaft of the centrifugal motor 201 drives a centrifugal rotating rod 202 fixed with the centrifugal motor to rotate, the centrifugal rotating rod 202 simultaneously drives a centrifugal sleeve 203 and a pushing sleeve 205 fixedly sleeved with the centrifugal rotating rod to rotate, the centrifugal sleeve 203 and the pushing sleeve 205 rotate to respectively drive a centrifugal fan blade 204 and a pushing rod 206 fixed with the centrifugal motor to rotate, the pushing rods 206 are provided with a plurality of groups, the length of the pushing rods is reduced in an equal ratio, the centrifugal fan blade 204 centrifugally accelerates the liquid material in the tissue culture incubator 1 from the bottom to form a suspension, the pushing rod 206 with the length of the pushing rods is reduced in an equal ratio pushes the suspension from the centrifugal rotating rod 202 to the periphery, and the absorption of the distribution liquid pump 10 is facilitated; wherein the centrifugal motor 201, the centrifugal rotating rod 202, the centrifugal sleeve 203, the centrifugal fan blade 204, the pushing sleeve 205 and the pushing rod 206 form a centrifugal pushing assembly 3;

the micro-material storage tube group 9 is composed of a liquid storage tube 901, a slider 902, an expansion rod 903 and an extrusion block 904, wherein the slider 902 is arranged in the liquid storage tube 901 in a sliding manner, the top end of the slider 902 is fixedly connected with the expansion rod 903, the top end of the expansion rod 903, which is far away from the slider 902, penetrates through the top wall of the liquid storage tube 901 in a sliding manner and extends to the outside of the liquid storage tube and is fixedly connected with the extrusion block 904, a liquid outlet 905 is formed in the bottom end face of the liquid storage tube 901, which is far away from the center, the extrusion block 904 is extruded downwards, the expansion rod 903 moves into the liquid storage tube 901 after the extrusion block 904 is extruded, the expansion rod 903 moves into the liquid storage tube 901 and drives the slider 902 fixed with the expansion rod to slide downwards along the inner wall of the liquid storage tube 901, the slider 902 extrudes the micro additive liquid in the liquid storage tube 901 after sliding downwards, when the liquid outlet 905 is blocked, the micro additive liquid is pressurized, and when the liquid outlet 905 is communicated, the liquid is pressurized to more easily circulated;

the control panel 102 starts a servo motor 824 arranged in a lifting box 823 to work and controls an output shaft thereof to rotate, the output shaft of the servo motor 824 drives a second rotating rod 825 fixed with the servo motor to rotate after rotating, the second rotating rod 825 drives a third bevel gear 826 fixedly sleeved with the second rotating rod to rotate after rotating, and the third bevel gear 826 is arranged between a first bevel gear 808 and a second bevel gear 811, so that when the control panel 102 controls the lifting air rod 822 to push the power box 801 to move downwards, the lifting air rod 822 pushes the power box 801 to move downwards so that the third bevel gear 826 is engaged with the second bevel gear 811, the rotating power of the third bevel gear 826 is transmitted to the second bevel gear 811, the second bevel gear 811 rotates in a single direction, the quantitative adding component 803 works and is positioned at different micro-material storage tube groups 9, meanwhile, the power box 801 moves downwards to press a return spring 829 to contract the return spring 829, after the transmission power is given to the second bevel gear 811, the control of the lifting air rod 822 stops controlling the lifting air rod 822, at this time, the third bevel gear 826 is engaged with the first bevel gear 811 engages with the first bevel gear under the action of the return spring 829, and then controls the output shaft to rotate in a positive direction or a constant pressure in a positive direction, and controls the first bevel gear 808 to rotate; wherein the lifting air rod 822, the lifting box 823, the servo motor 824, the second rotating rod 825, the third bevel gear 826, the second connecting loop bar 827, the second limit sliding rod 828 and the return spring 829 form the switching driving assembly 804;

the constant pressure control assembly 802 comprises a lifting panel 805, a plurality of embedding grooves 810 are formed in the lifting panel 805, the embedding grooves 810 are formed in the outer ring side of the bottom of the lifting panel 805, the embedding grooves 810 are distributed in an annular array by taking the circle center of the lifting panel 805 as the center, the extrusion blocks 904 are movably embedded in the embedding grooves 810, the lifting panel 805 is sleeved with a first limiting slide bar 806 and a threaded rotating bar 807, the first limiting slide bar 806 is in sliding connection with the lifting panel 805, a plurality of first limiting slide bars 806 are arranged, the first limiting slide bars 806 are distributed in an annular array by taking the circle center of the lifting panel 805 as the center, the bottom end of the first limiting slide bar 806 is fixedly connected with the top end face of the limiting box 7, the threaded rotating bar 807 is in threaded connection with the lifting panel 805, anti-disengaging blocks 809 are arranged at the top ends of the threaded rotating bar 807 and the first limiting slide bars 806, the bottom end faces of the threaded rotating bar 809 are movably abutted against the lifting panel 805, the lifting panel 805 are connected with the bottom end faces of the threaded rotating bar 809, the bottom end of the threaded rotating bar 809, the threaded rotating bar 808 is movably abutted against the lifting panel 805, the outer wall of the lifting panel 805, the threaded rotating bar 808 extends to the outer wall of the limiting box 7 and is fixedly sleeved with a first bevel gear 808, and the first bevel gear 808 is movably engaged with the switching drive assembly 804;

when the micro material storage tube group 9 is installed, the extrusion block 904 is embedded into an embedding groove 810 formed in the lifting panel 805 from the side, the forward rotation of the first bevel gear 808 is indirectly controlled to drive the extrusion block 904 in threaded sleeve connection with the first bevel gear to slide downwards along the first limiting slide bar 806, the downward sliding of the extrusion block 904 extrudes the extrusion block 904 embedded in the extrusion block to move downwards, the downward movement of the extrusion block 904 enables the pressure in the micro material storage tube group 9 to be gradually increased until the pressure in the micro material storage tube group 9 is constant to a preset value, meanwhile, the pressure is applied to the micro material storage tube group 9 to enable the micro material storage tube group to be installed more stably, the forward rotation of the first bevel gear 808 is indirectly controlled to achieve the purpose, the reverse rotation of the first bevel gear 808 is indirectly controlled to enable the lifting panel to move upwards until the lifting panel 805 is abutted to the anti-disengaging block 809, the lifting panel 805 cannot be disengaged, and the detachability of the micro material storage tube group 9 is achieved;

the quantitative adding assembly 803 comprises a second bevel gear 811, the second bevel gear 811 is movably engaged with the switching driving assembly 804, the second bevel gear 811 is fixedly connected with a first rotating rod 812, the first rotating rod 812 is rotatably arranged, one end of the first rotating rod 812, which is far away from the second bevel gear 811, sequentially penetrates through the inner wall of the power box 801 and the outer wall of the limiting box 7, extends into the limiting box 7 and is fixedly sleeved with a first connecting sleeve rod 813, one end of the first connecting sleeve rod 813, which is far away from the first rotating rod 812, is provided with a micro-control motor 814, an output shaft of the micro-control motor 814 is fixedly sleeved with a driving gear 815, the driving gear 815 is movably engaged and connected with a quantitative gear 816, a plurality of quantitative gears 816 are arranged, the quantitative gears 816 are distributed according to an annular array by taking the central axis of the hollow rotating rod 821, and the quantitative gears 816 are arranged in one-to-one correspondence with the micro-material storage tube group 9;

a hollow rotating rod 821 is rotatably sleeved on the quantitative gear 816, the hollow rotating rod 821 is fixedly arranged on the bottom wall of the limiting box 7, two ends of the quantitative gear 816 are abutted with limiting sealing wheels 818, the limiting sealing wheels 818 are fixedly sleeved on the outer end of the hollow rotating rod 821, a clamping elastic sleeve 819 is arranged at the top end of one of the limiting sealing wheels 818, the clamping elastic sleeve 819 is clamped with the bottom of the liquid storage tube 901, a fixed arc sleeve 820 is fixedly connected with the outer end of the limiting sealing wheel 818, one end, far away from the limiting sealing wheel 818, of the fixed arc sleeve 820 is fixedly connected with the inner wall of the limiting box 7, the quantitative gear 816 is provided with L-shaped leakage holes 817 which are movably communicated with the liquid outlet 905, the L-shaped leakage holes 817 are distributed in a circular array by centering on the circle center of the quantitative gear 816, the hole diameters of the L-shaped leakage holes 817 are increased in an equal ratio, the maximum hole diameter of the L-shaped leakage holes 817 is equal to the hole diameter of the liquid outlet 905, and the large hole 817 of the side of the liquid outlet 905 are arranged;

when different trace additive liquid is required to be quantitatively added, the second bevel gear 811 is indirectly controlled to rotate in a single direction to drive the first rotating rod 812 fixed with the second bevel gear to rotate, the first rotating rod 812 drives the first connecting sleeve rod 813 fixed with the first rotating rod to rotate, the micro-control motor 814 arranged on the first connecting sleeve rod 813 after rotating deflects, the micro-control motor 814 drives the driving gear 815 fixedly sleeved with the output shaft of the micro-control motor to deflect after being deflected, so that the driving gear 815 is meshed with the quantitative gear 816 and drives the quantitative gear to rotate, the different quantitative gears 816 correspondingly store micro-material storage pipe groups 9 with different trace additive liquid for target ingredients, then the control panel 102 selects a target L-shaped leakage hole 817 with a proper aperture according to the required ingredient, then the micro-control motor 814 is started to work and controls the output shaft of the micro-control motor 814 to rotate to a fixed number of turns, after the output shaft of the micro-control motor 814 rotates to a certain number of turns, the quantitative gear 816 engaged with the output shaft is driven to rotate for a certain angle, after the quantitative gear 816 rotates for a certain angle, at this time, the target L-shaped leak 817 of the quantitative gear 816 is just aligned with the liquid outlet 905, so that the liquid outlet 905, the L-shaped leak 817 and the side port formed on the hollow rotating rod 821 are sequentially communicated, the target micro-additive liquid is added into the hollow rotating rod 821 through the liquid outlet 905, the L-shaped leak 817 and the side port, and then the target micro-additive liquid flows into the diversion hopper 6 through the hollow rotating rod 821, after the target micro-additive liquid is added, the steps are repeated, different target micro-additive liquids required to be prepared are sequentially added into the diversion hopper 6, and then the diversion hopper 6 gathers the target micro-additive liquid into the suspension centrifugal box 2 through the diversion pipe 12, thereby completing the quantitative control adding work of the different micro-additive liquids, and enabling the aperture of the L-shaped leak 817 to be increased in an equal ratio, the quantitative addition of the trace additive solution is easy to control;

the working principle is as follows:

step one, inserting a micro-material storage tube group 9 from a top opening formed in a suspension centrifugal box 2 according to a formula of tissue culture requirements, then embedding a pressing block 904 side of the micro-material storage tube group 9 into a clamping groove 810 of a lifting panel 805 of a constant pressure control assembly 802, then sequentially installing the micro-material storage tube group 9 which is required to store target micro additive liquid according to the formula of the requirements, then placing tissue blocks of orchid into a tissue placing opening 501 formed in a tissue culture installation box 5, supporting the tissue blocks of the orchid by a liquid seepage net 502 at the bottom of the tissue placing opening 501, then opening a hinged arc door 104, placing the tissue culture installation box 4 into the top of the tissue culture installation box 5, supporting the tissue culture installation box 4 up the tissue culture net 5, and closing the hinged arc door 104 after the tissue culture installation box 5 is placed;

step two, after the micro-material storage pipe is installed, a liquid inlet throttle valve 14 is opened to enable main liquid of the formula to flow into the suspension centrifugal box 2 from the outside through a liquid inlet pipe 13, and meanwhile, a switching driving assembly 804 and a constant pressure control assembly 802 are controlled to conduct transmission work, so that micro-addition liquid pressure in an extrusion micro-material storage pipe group 9 is constant, the micro-material storage pipe group 9 is further embedded into a quantitative addition assembly 803, and the stability of the micro-material storage pipe group 9 is improved;

when the pressure of the trace additive in the trace additive storage pipe group 9 reaches a preset value, the switching drive component 804 is controlled to be separated from the constant pressure control component 802 and is in transmission connection with the quantitative addition component 803, then after the quantitative addition component 803 works, the diversion hopper 6 is in through connection with the trace additive storage pipe group 9, so that different corresponding trace additive solutions in different trace additive storage pipe groups 9 sequentially and quantitatively flow into the diversion hopper 6 according to the formula requirements, and then the trace additive solutions are guided into the suspension centrifugal box 2 through the diversion pipe 12 in through connection with the lowest point of the diversion hopper 6;

thirdly, after the mixed liquid in the suspension centrifugal box 2 reaches a preset height, starting the centrifugal pushing assembly 3 to work, further mixing and scattering the mixed liquid, fully contacting the mixed liquid and generating suspension, wherein the suspension is suspended on the top surface of the mixed liquid and then gradually pushed to the periphery from the middle part of the mixed liquid, and at the moment, starting the distribution liquid pump 10 to continuously suck the suspension through a conduit and equally distribute the suspension to the tissue culture installation boxes 4 which are equidistantly arranged through the distribution pipe 11, and continuously pushing the suspension to the connection part of the distribution liquid pump 10 under the action of the centrifugal pushing assembly 3, so that the suspension is more conveniently sucked; wherein all install the check valve that prevents the suspension backward flow on the group banks up the install bin 4 with earth, prevent liquid intercommunication effect, the check valve is installed in the tip of the distribution pipe 11 of far away distribution liquid pump 10, the quantity of check valve and the quantity adaptation that banks up the install bin 4 with earth, the check valve is not drawn.

Step four, after the suspension flows into the tissue culture installation box 4 and is gradually increased, the suspension gradually rises and flows into the tissue placement opening 501 through the liquid seepage net 502, when the suspension covers the local part of the tissue block of the orchid, the distribution liquid pump 10 is closed, the suspension in the tissue block of the orchid gradually becomes less in the process of rooting, sprouting and growing gradually, so that the distribution liquid pump 10 needs to be opened in real time to convey the suspension, the suspension is quantified, the suspension consumed in the process of rooting, sprouting and growing of the tissue block of the orchid is different, the suspension needs to be dynamically sucked according to the growth period of the tissue culture of the orchid seedling, the liquid in the tissue culture installation box 4 is kept in a quantified state, and the full and rapid growth of the orchid seedling in the tissue culture process is ensured;

step five, after the tissue culture of the orchid seedlings is finished, opening the hinged arc door 104, then taking the tissue culture screen plate 5 and replacing the tissue culture screen plate 5 with a new tissue culture screen plate 5 in which orchid tissue blocks are placed, and thus continuously collecting the orchid seedlings which are subjected to tissue culture growth;

by integrating the technical scheme, the tissue culture device realizes the efficient generation of the tissue culture of the orchid seedlings on the basis of the continuous tissue culture of the orchid seedlings, and realizes the micro-pressure control of the micro-addition liquid, the quantitative control of the addition of the micro-addition liquid and the selection control of the micro-addition liquid with different required formulas through the cooperation of the constant-pressure control component 802, the quantitative addition component 803 and the switching drive component 804, so that the tissue culture device can be widely adapted to different nutritional requirements of various tissue tissues of the orchid, and the tissue culture adaptability of the device is wider.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered as the technical solutions and the inventive concepts of the present invention within the technical scope of the present invention.

Claims

1. Orchid seedling group banks up device with earth based on intelligent regulation and control, including group banks up thermostated container (1) with earth, group banks up thermostated container (1) internal fixation with earth and is equipped with suspension centrifugation case (2) and group banks up install bin (4) with earth, its characterized in that, the diapire of group banks up thermostated container (1) with earth is located in suspension centrifugation case (2), and installs centrifugation promotion subassembly (3) on suspension centrifugation case (2), centrifugation promotion subassembly (3) are installed on thermostated container (1), group banks up install bin (4) with earth and is equipped with a plurality ofly, and the top surface activity card of group banks up install bin (4) with earth is equipped with group and banks up otter board (5) with earth, a plurality of groups that are used for placing orchid tissue place mouth (501) are seted up to otter board (5), and the bottom department of tissue place mouth (501) is equipped with the sepage net (502) that are used for holding up orchid tissue with earth, ooze the bottom of group culture otter board (5) with earth and fix the bottom of locating group culture otter board (5) with earth;

the top of the suspension centrifugal box (2) is connected with a distribution liquid pump (10) through a pipeline in a through manner, a liquid inlet of the distribution liquid pump (10) is connected with the top of the suspension centrifugal box (2) through manner, a liquid outlet of the distribution liquid pump (10) is connected with a distribution pipe (11) through manner, one end of the distribution pipe (11) far away from the distribution liquid pump (10) is connected with the bottom center of the tissue culture installation box (4) through manner, two sides of the bottom of the suspension centrifugal box (2) are connected with a flow guide pipe (12) and a liquid inlet pipe (13) through manner, a liquid inlet throttle valve (14) is installed on the liquid inlet pipe (13), one end of the flow guide pipe (12) is connected with a flow guide hopper (6) through manner, the top end of the flow guide hopper (6) is fixedly connected with a limit box (7), the limit box (7) is connected with the flow guide hopper (6) through manner, the limit box (7) is provided with a micro-adding control structure (8) and a micro-material storage pipe group (9), the micro-adding control structure (8) comprises a power box (801), a constant-pressure control component (802), a quantitative adding component (803 and a power switching drive component (804), a plurality of micro-storage pipes (9) which are embedded in the micro-storage box (7) and a plurality of micro-material storage pipe group (7) which is arranged in the micro-storage box (801), the micro-material storage tube group (9) is distributed according to an annular array by taking the circle center of the limiting box (7) as the center, the micro-material storage tube group (9) is matched with the quantitative adding assembly (803), the constant pressure control assembly (802) is matched and abutted with the micro-material storage tube group (9), and the constant pressure control assembly (802) and the quantitative adding assembly (803) are movably connected with the switching driving assembly (804) in a transmission manner;

the centrifugal pushing assembly (3) is composed of a centrifugal motor (201), a centrifugal rotating rod (202), a centrifugal sleeve (203), centrifugal fan blades (204), a pushing sleeve (205) and a pushing rod (206), the centrifugal motor (201) is fixedly arranged at the bottom end of the tissue culture incubator (1), the centrifugal rotating rod (202) is rotatably arranged in the suspension centrifugal incubator (2), one end of the centrifugal rotating rod (202) sequentially penetrates through the inner wall of the suspension centrifugal incubator (2) and the inner wall of the tissue culture incubator (1) to extend to the outside of the tissue culture incubator and is fixedly connected with an output shaft of the centrifugal motor (201), the centrifugal sleeve (203) and the pushing sleeve (205) are arranged at the outer end of the centrifugal rotating rod (202), the centrifugal fan blades (204) are fixedly sleeved at the outer end of the centrifugal sleeve (203), the pushing rod (206) is arranged at the outer end of the pushing sleeve (205), and the pushing rod (206) is arranged above the centrifugal fan blades (204);

the micro-material storage tube group (9) is composed of a liquid storage tube (901), a sliding block (902), an expansion rod (903) and an extrusion block (904), the sliding block (902) is arranged in the liquid storage tube (901) in a sliding mode, the top end of the sliding block (902) is fixedly connected with the expansion rod (903), the top end, far away from the sliding block (902), of the expansion rod (903) penetrates through the top wall of the liquid storage tube (901) in a sliding mode to extend to the outside of the liquid storage tube and is fixedly connected with the extrusion block (904), and a liquid outlet (905) is formed in the position, far away from the center, of the bottom end face of the liquid storage tube (901);

the constant pressure control assembly (802) includes a riser panel (805), the riser panel (805) defining a plurality of retention slots (810), the retention groove (810) is provided on the bottom outer circumferential side of the riser panel (805), and the retention grooves (810) are distributed in a circular array centered on the center of the riser panel (805), the extrusion block (904) is movably embedded in the embedding groove (810), the lifting panel (805) is sleeved with a first limiting slide rod (806) and a threaded rotating rod (807), the first limit slide bar (806) is connected with the lifting panel (805) in a sliding way, a plurality of first limit slide bars (806) are arranged, and the first stop bars (806) are centered on the center of the lifting panel (805) and distributed in an annular array, the bottom end of the first limit slide bar (806) is fixedly connected with the top end surface of the limit box (7), the threaded rotating rod (807) is in threaded connection with the lifting panel (805), the top ends of the threaded rotating rod (807) and the first limiting sliding rod (806) are provided with anti-falling blocks (809), the bottom end surface of the anti-falling block (809) is movably abutted with the lifting panel (805), the bottom end of the screw thread rotating rod (807) rotatably penetrates through the outer wall of the limit box (7) to extend to the inside of the limit box and is fixedly sleeved with a first bevel gear (808), the first bevel gear (808) is movably meshed with a switching driving component (804);

the quantitative adding assembly (803) comprises a second bevel gear (811), the second bevel gear (811) is movably meshed with the switching driving assembly (804), a first rotating rod (812) is fixedly connected with the second bevel gear (811), the first rotating rod (812) is rotatably arranged, one end, far away from the second bevel gear (811), of the first rotating rod (812) sequentially penetrates through the inner wall of the power box (801) and the outer wall of the limiting box (7) to extend into the limiting box (7) and is fixedly sleeved with a first connecting sleeve rod (813), one end, far away from the first rotating rod (812), of the first connecting sleeve rod (813) is provided with a micro-control motor (814), an output shaft of the micro-control motor (814) is fixedly sleeved with a driving gear (815), the driving gear (815) is movably meshed with a quantitative gear (816), the quantitative gear (816) is provided with a plurality of quantitative gears, the quantitative gears (821) are distributed according to an annular array by taking the central axis of the hollow rotating rod (821) as the outer end of the central axis of the hollow rotating rod (821), the quantitative gears (816) are arranged in one-to-one correspondence with the micro-material storage tube groups (9), the quantitative gears (821) are arranged, the two fixed rotating rod (821) are sleeved with the sealing ends (816), the limiting rod (821) of the limiting box (821), the hollow rotating rod (818), the sealing rod (821) and are abutted to be abutted to the limiting box (818), the top end of one of the limiting sealing wheels (818) is provided with a clamping elastic sleeve (819), the clamping elastic sleeve (819) is clamped with the bottom of the liquid storage pipe (901), the outer end of the limiting sealing wheel (818) is fixedly connected with a fixed arc sleeve (820), one end, far away from the limiting sealing wheel (818), of the fixed arc sleeve (820) is fixedly connected with the inner wall of the limiting box (7), the quantifying gear (816) is provided with an L-shaped leakage hole (817), the L-shaped leakage hole (817) is movably connected with the liquid outlet (905) in a penetrating manner, the L-shaped leakage hole (817) is provided with a side flow port with the hollow rotating rod (821), and the side flow port is movably connected with the liquid outlet (905) in a penetrating manner through the L-shaped leakage hole (817);

the L-shaped leakage holes (817) are arranged in a plurality of numbers, the L-shaped leakage holes (817) are distributed in an annular array by taking the circle center of the quantitative gear (816) as the center, the aperture of the L-shaped leakage holes (817) is increased in an equal ratio, the maximum aperture of the L-shaped leakage holes (817) is equal to the aperture of the liquid outlet (905), and the apertures of the liquid outlet (905) and the side flow port are arranged in an equal size;

the switching driving assembly (804) comprises a lifting box (823), the lifting box (823) is arranged in the power box (801) in a sliding mode, the lifting box (823) is provided with a lifting air rod (822), a second connecting loop bar (827) and a return spring (829), the lifting air rod (822) is arranged on the bottom wall of the power box (801), one end of the second connecting loop bar (827) is fixedly connected with the power box (801), and the other part of the second connecting loop bar (827) is sleeved with a second limit slide bar (828), the second limit slide bar (828) is fixedly arranged in the power box (801), the return spring (829) is fixedly arranged at the bottom end of the power box (801), and the other end of the return spring (829) is fixedly arranged on the bottom wall of the power box (801), a servo motor (824) is fixedly arranged in the power box (801), the output shaft of the servo motor (824) is fixedly connected with a second rotating rod (825), the second rotating rod (825) is rotatably arranged in the lifting box (823), one end of the second rotating rod (825) penetrates through the inner wall of the lifting box (823) to extend to the outside of the lifting box and is fixedly sleeved with a third bevel gear (826), the first bevel gear (808) and the second bevel gear (811) are arranged oppositely, and the symmetrical sides of the third bevel gear (826) are in clearance engagement with the first bevel gear (808) and the second bevel gear (811), respectively.

2. The orchid seedling tissue culture device based on intelligent regulation and control as claimed in claim 1, wherein a base (101) is installed at the bottom of the tissue culture incubator (1), a plurality of hinged arc doors (104) are installed on the outer wall of the tissue culture incubator (1), a hinged arc door (104) is correspondingly arranged at each tissue culture installation box (4), a door handle (105) is installed on each hinged arc door (104), a control panel (102) is installed at the outer end of the tissue culture incubator (1), and a starting button (103) and a display screen are installed on each control panel (102).