CN114602325B - Sampling method, control method and system of disk type environment DNA filter cake replacement device - Google Patents
Sampling method, control method and system of disk type environment DNA filter cake replacement device Download PDFInfo
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Abstract
The invention provides a sampling method of a wheel disc type environment DNA filter cake replacement device, which comprises the steps of executing N times of rotation actions through a first driving device, wherein N is greater than or equal to 4; when the first driving device executes the first rotation action, the first hole is rotated to a first position to load the filter cake; when the first driving device executes the second rotation action, the first hole is rotated to a second position for water sample collection, and the second hole is rotated to the first position; when the first driving device executes the third rotation action, the first hole is rotated to a third position to unload the filter cake, and the second hole is rotated to a second position and the third hole is rotated to a first position; when the first driving device executes the fourth rotation action, the first hole is rotated to a fourth position to perform the pre-loading action, and the second hole is rotated to a third position, the third hole is rotated to a second position and the fourth hole is rotated to a first position; to allow replacement and sampling of the filter membrane through a plurality of wells.
Description
Technical Field
The invention belongs to the technical field of agriculture, and particularly relates to a sampling method, a control method and a system of a disk type environment DNA filter cake replacement device.
Background
Along with the advancement of new strategies for global biodiversity management and the implementation of the united nations 'biodiversity convention', the importance of biodiversity investigation and research is becoming more and more prominent. With the development of biodiversity research, large-scale and high-frequency metagenomic analysis of environmental DNA and microorganisms in water is generally required, which requires large-scale filtration of water samples and high-frequency replacement of filter membranes. Different from the common filter membrane replacement scene, in the environment DNA sampling or microorganism sampling scene, special attention is required to avoid cross contamination between samples and avoid introducing new pollution in the operation process, thereby ensuring the independence of the samples and the accuracy of detection results. In the past, the method for simply and directly replacing the filter membrane is that after a water sample is filtered by a wafer filter membrane, an experimenter manually opens the filter head and takes out the filter membrane containing the sample by using clean tweezers, then the filter head is manually reinstalled after the filter membrane is replaced by a new wafer filter membrane by using the clean tweezers. Whether applied to long-term continuous monitoring at a fixed site or to sequential sampling of multiple sites following a survey vessel, this means a large-scale high-frequency disc filter replacement operation. The major problems and disadvantages of the prior art include: due to the current manual replacement of the disc filter membrane, the operation is complicated, and different experimenters may introduce errors due to different operation methods. And the operation flow of disassembling, film taking, film changing and reinstalling is excessively consumed, so that a great deal of time and labor are wasted, and the high-frequency sampling is not facilitated. Again, manual replacement of the filters also tends to introduce contamination, causing deviations in the sample survey results. Finally, the manual filter membrane replacement mode is not separated from the operation of people, is not beneficial to automatic sampling and filtering, and is also not beneficial to standardized long-term continuous sampling or online continuous monitoring activities. The scheme of replacing the filter membranes by pneumatic adsorption used in other fields is easy to cause cross contamination between the filter membranes. The scheme of rotating disc type filter membrane replacement used in other fields has the advantages that the number of the filter membranes which can be accommodated is small, and the large-scale high-frequency application of the scene of disc filter membrane replacement cannot be met.
That is, in the prior art, the filter membrane is replaced manually, so that there are drawbacks in that the operation is complicated, errors may be introduced by different experimenters due to different operation methods, and the replacement efficiency is low.
Therefore, how to improve the replacement speed of the filter membrane and reduce the error occurring in the replacement process, so as to improve the replacement efficiency of the filter membrane is a technical problem that needs to be solved by those skilled in the art.
Disclosure of Invention
The sampling method of the disk type environment DNA filter cake replacement device provided by the invention is used for at least solving the technical problems;
in order to solve the above-mentioned problems, a first aspect of the present invention provides a sampling method of a disk-type environmental DNA filter cake replacement device, the filter cake being assembled as a filter cake of unitary structure by an annular housing, the replacement device comprising: the device comprises a magazine type mechanism, a sampling area and a rotary table, wherein the rotary table is disc-shaped and is driven to rotate by a first driving device arranged at the center of the rotary table, and a first hole, a second hole, a third hole and a fourth hole which are matched with the filter cake are formed in the rotary table; the magazine mechanism and the sampling area are correspondingly arranged on paths of the first hole, the second hole, the third hole and the fourth hole which rotate around the circle center; a plurality of filter cakes are arranged in the magazine mechanism in an up-down overlapping way, and a channel which just passes through one filter cake is reserved at the bottom of the magazine mechanism; the sampling area is used for collecting a water sample of the filter cake; the sampling method comprises the following steps: executing N times of rotation actions through the first driving device, wherein N is greater than or equal to 4; when the first driving device executes a first rotation action, the first hole is rotated to a first position to load a filter cake; when the first driving device executes a second rotation action, the first hole is rotated to a second position, water sample collection is performed, and the second hole is rotated to the first position; when the first driving device executes a third rotation action, the first hole is rotated to a third position, filter cake unloading is carried out, the second hole is rotated to the second position, and the third hole is rotated to the first position; when the first driving device executes a fourth rotation action, the first hole is rotated to a fourth position to perform a pre-loading action, and the second hole is rotated to the third position, the third hole is rotated to the second position, and the fourth hole is rotated to the first position; the first position is the through hole position of the magazine type mechanism, and the second position is the position of the sampling area.
In a first aspect, when the first driving device performs a second rotation, the first hole is rotated to a second position, and water sample collection includes: when the first driving device executes the second rotation action, the first hole is rotated to the second position, and water sample collection is performed for preset time.
In a first aspect, when the first driving device performs a second rotation, the first hole is rotated to a second position, and after water sample collection, the sampling method further includes: and detecting the water sample acquisition result, stopping the water sample acquisition if the result reaches a preset detection value, and continuing to acquire the water sample if the result does not reach the preset detection value.
In a first aspect, the performing the pre-load action comprises: and cleaning and sterilizing by using a sterilizing liquid.
In a second aspect, the present invention provides a control method of a disc-type environmental DNA filter cake replacement device, where the control method is applied to the sampling method described in any one of the above, and the replacement device further includes: the control device is correspondingly connected with the detection device and the first driving device, and the control method comprises the following steps: detecting positions of the first hole, the second hole, the third hole and the fourth hole by the detection device, and generating position information; and acquiring the position information through the control device, judging whether the first hole, the second hole, the third hole and the fourth hole reach preset positions according to the position information, if so, controlling the first driving device to stop executing the rotation action, and if not, controlling the first driving device to continue executing the rotation action.
In a second aspect, the detecting, by the detection device, the positions of the first hole, the second hole, the third hole, and the fourth hole includes: the positions of the first hole, the second hole, the third hole, and the fourth hole are detected by an electronic eye.
In a second aspect, the electronic grabbing position of the electronic eye is fixedly mapped on one of the orientations of the turntable, and before the positions of the first hole, the second hole, the third hole and the fourth hole are detected by the electronic eye, the control method further includes: a plurality of marks which are in one-to-one correspondence with the first hole, the second hole, the third hole and the fourth hole and can be identified by the electronic eyes are arranged on the turntable, and one of the marks is used as a starting mark for capturing by the electronic eyes; the detecting, by the electronic eye, the positions of the first hole, the second hole, the third hole, and the fourth hole includes: detecting whether the mark exists at the position corresponding to the mapping azimuth through the electronic eye, and generating mark information; the step of obtaining the position information through the control device, judging whether the first hole, the second hole, the third hole and the fourth hole reach a preset position according to the position information, if yes, controlling the first driving device to stop executing the rotation action, and if not, controlling the first driving device to continue executing the rotation action comprises the following steps: and acquiring the marking information through the control device, judging whether the first hole, the second hole, the third hole and the fourth hole reach preset positions according to the marking information, if so, controlling the first driving device to stop executing preset rotation actions, and if not, controlling the first driving device to continue executing the rotation actions.
In a second aspect, after the setting of the marks on the turntable, the marks are in one-to-one correspondence with the first hole, the second hole, the third hole and the fourth hole and can be recognized by an electronic eye, the control method further includes: numbering the marks, enabling the first holes to correspond to a first number, enabling the second holes to correspond to a second number, enabling the third holes to correspond to a third number and enabling the fourth holes to correspond to a fourth number; taking one of the marks as a starting mark for electronic eye capturing; the detecting, by the electronic eye, the positions of the first hole, the second hole, the third hole, and the fourth hole includes: detecting whether the mark exists at the position corresponding to the mapping azimuth through the electronic eye; if yes, the control device controls the first driving device to execute a preset rotation action, and if not, the control device controls the first driving device to stop rotating, wherein the control device comprises the following steps: taking the number one as a starting mark for the electronic eye capture; and detecting whether the mark of the position corresponding to the mapping azimuth is a preset number or not through the electronic eye, if so, controlling the first driving device to stop driving by the control device, and otherwise, controlling the first driving device to execute the rotating action by the control device so as to enable the hole position of the corresponding number to rotate to the mapping azimuth.
In a second aspect, controlling the first driving device to perform the rotation action, and rotating the hole sites with corresponding numbers to the mapping directions includes: acquiring the number information of the last hole site through the control device, and calculating the number information of the next hole site according to the number information of the last hole site; and controlling the first driving device to execute the rotating action through the control device according to the calculated serial number information of the next hole site, so that the hole site with the serial number corresponding to the serial number information of the next hole site rotates to the mapping azimuth.
In a third aspect, the invention provides a sampling system of a disk-type environmental DNA filter cake replacement device, and the sampling system is applied to the sampling method of the disk-type environmental DNA filter cake replacement device described in any one of the above.
The beneficial effects are that: the invention provides a sampling method of a disk type environment DNA filter cake replacement device, which comprises the following steps that a first driving device executes N times of rotation actions, wherein N is greater than or equal to 4, and when the first driving device executes the action of 4, the sampling method comprises the following steps: when the first driving device executes the first rotation action, the first hole is rotated to a first position to load the filter cake; when the first driving device executes the second rotation action, the first hole is rotated to a second position for water sample collection, and the second hole is rotated to the first position; when the first driving device executes the third rotation action, the first hole is rotated to a third position to unload the filter cake, and the second hole is rotated to a second position and the third hole is rotated to a first position; when the first driving device executes the fourth rotation action, the first hole is rotated to a fourth position to perform the pre-loading action, and the second hole is rotated to a third position, the third hole is rotated to a second position and the fourth hole is rotated to a first position; the first position is the through hole position of the magazine type mechanism, the second position is the position of the sampling area, and in sum, the first hole, the second hole, the third hole and the fourth hole are sequentially subjected to filter cake loading, water sample collection, filter cake unloading and preparation working procedures through the rotation action of the first driving device, so that the effect of filter cake alternate replacement is achieved, and the technical problem of replacement errors caused by different methods in the manual filter membrane replacement in the prior art is solved.
Drawings
In order to more clearly illustrate the embodiments of the present description or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a block flow diagram of a sampling method of a disc-type environmental DNA filter cake replacement device according to an embodiment of the invention;
FIG. 2 is a flow chart of a method for controlling a disc-type environmental DNA filter cake replacement device according to an embodiment of the invention.
Detailed Description
The following description of the embodiments of the present invention will be made more apparent and fully hereinafter with reference to the accompanying drawings, in which some, but not all embodiments of the invention are shown. Embodiments of the present invention are intended to be within the scope of the present invention as defined by the appended claims.
Embodiment one:
as shown in fig. 1, in a first embodiment, a sampling method of a disk-type environmental DNA filter cake replacement device is provided, in which a filter cake is assembled into a filter cake of an integral structure by an annular housing, the replacement device includes: the device comprises a magazine type mechanism, a sampling area and a rotary table;
the rotary table is disc-shaped and is driven to rotate by a first driving device arranged at the center of the rotary table, and a first hole, a second hole, a third hole and a fourth hole which are matched with the filter cake are formed in the rotary table;
the magazine mechanism and the sampling area are correspondingly arranged on paths of the first hole, the second hole, the third hole and the fourth hole which rotate around the circle center;
a plurality of filter cakes are overlapped up and down in the magazine mechanism, and a channel which just passes through one filter cake is reserved at the bottom of the magazine mechanism; the sampling area is used for collecting a water sample of the filter cake;
the control method comprises the following steps:
executing N times of rotation actions through the first driving device, wherein N is greater than or equal to 4;
when the first driving device executes the first rotation action, the first hole is rotated to a first position to load the filter cake;
when the first driving device executes the second rotation action, the first hole is rotated to a second position for water sample collection, and the second hole is rotated to the first position;
when the first driving device executes the third rotation action, the first hole is rotated to a third position to unload the filter cake, and the second hole is rotated to a second position and the third hole is rotated to a first position;
when the first driving device executes the fourth rotation action, the first hole is rotated to a fourth position to perform the pre-loading action, and the second hole is rotated to a third position, the third hole is rotated to a second position and the fourth hole is rotated to a first position;
the first position is the through hole position of the magazine type mechanism, and the second position is the position of the sampling area.
In the technical solution of the first embodiment, the first driving device executes N rotation actions, where N is greater than or equal to 4, and when the first driving device executes the action 4, the method includes the following steps: when the first driving device executes the first rotation action, the first hole is rotated to a first position to load the filter cake; when the first driving device executes the second rotation action, the first hole is rotated to a second position for water sample collection, and the second hole is rotated to the first position; when the first driving device executes the third rotation action, the first hole is rotated to a third position to unload the filter cake, and the second hole is rotated to a second position and the third hole is rotated to a first position; when the first driving device executes the fourth rotation action, the first hole is rotated to a fourth position to perform the pre-loading action, and the second hole is rotated to a third position, the third hole is rotated to a second position and the fourth hole is rotated to a first position; the first position is the through hole position of the magazine type mechanism, the second position is the position of the sampling area, and in sum, the first hole, the second hole, the third hole and the fourth hole are sequentially subjected to filter cake loading, water sample collection, filter cake unloading and preparation working procedures through the rotation action of the first driving device, so that the effect of filter cake alternate replacement is achieved, and the technical problem of replacement errors caused by different methods in the manual filter membrane replacement in the prior art is solved.
Further, for the step of rotating the first hole to the second position when the first driving device performs the second rotation, the first embodiment further provides an embodiment, which includes: when the first driving device executes the second rotation action, the first hole is rotated to a second position, and water sample collection is performed for preset time, so that the sampling is ensured to be sufficient;
in a further aspect, the present embodiment provides an embodiment of the water sample collecting method, when the first driving device performs the second rotation, the first hole is rotated to the second position, and the embodiment includes: and detecting a water sample acquisition result, stopping water sample acquisition if the result reaches a preset detection value, and continuing water sample acquisition if the result does not reach the preset detection value.
Specifically, for the step of performing the pre-loading operation in the first embodiment, the first embodiment proposes an implementation manner, which includes: and cleaning and sterilizing by using a sterilizing liquid.
Embodiment two:
the second embodiment of the invention provides a control method of a disc-type environment DNA filter cake replacement device, the control method is applied to the sampling method of the disc-type environment DNA filter cake replacement device of any one of the above, and the replacement device further comprises: the control device is correspondingly connected with the detection device and the first driving device; the control method comprises the following steps:
detecting positions of the first hole, the second hole, the third hole and the fourth hole by a detection device, and generating position information;
and acquiring position information through the control device, judging whether the first hole, the second hole, the third hole and the fourth hole reach preset positions according to the position information, if so, controlling the first driving device to stop executing the rotation action, and if not, controlling the first driving device to continue executing the rotation action.
Further, for the step of detecting the positions of the first hole, the second hole, the third hole and the fourth hole by the detection device in the second embodiment, the second embodiment further proposes an implementation manner, which includes: the positions of the first hole, the second hole, the third hole, and the fourth hole are detected by the electronic eye.
Further, before the step of detecting the positions of the first hole, the second hole, the third hole and the fourth hole by the electronic eye in the second embodiment, the second embodiment further proposes an implementation manner, which includes: a plurality of marks which are in one-to-one correspondence with the first hole, the second hole, the third hole and the fourth hole and can be identified by the electronic eyes are arranged on the turntable; taking one of the marks as a starting mark for electronic eye capture; detecting the positions of the first hole, the second hole, the third hole, and the fourth hole by the electronic eye includes: detecting whether a mark exists at a position corresponding to the mapping azimuth through an electronic eye, and generating mark information; and in the second embodiment, the position information is obtained by the control device, whether the first hole, the second hole, the third hole and the fourth hole reach the preset position is determined according to the position information, if yes, the first driving device is controlled to stop executing the rotation action, if not, the first driving device is controlled to continue executing the rotation action, and in this embodiment, an implementation manner is further provided: the control device acquires the marking information, judges whether the first hole, the second hole, the third hole and the fourth hole reach preset positions according to the marking information, if so, controls the first driving device to stop executing preset rotation motions, and if not, controls the first driving device to continue executing rotation motions.
Further, for the second embodiment, after the plurality of marks, which are in one-to-one correspondence with the first hole, the second hole, the third hole and the fourth hole and can be identified by the electronic eye, are provided on the turntable, the control method further includes: numbering the marks, enabling the first holes to correspond to the first number, enabling the second holes to correspond to the second number, enabling the third holes to correspond to the third number and enabling the fourth holes to correspond to the fourth number; taking one of the marks as a starting mark for electronic eye capture; detecting the positions of the first hole, the second hole, the third hole, and the fourth hole by the electronic eye includes: detecting whether a mark exists at a position corresponding to the mapping azimuth through an electronic eye; if yes, the control device controls the first driving device to execute a preset rotation action, and if not, the control device controls the first driving device to stop rotating, wherein the control device comprises the following steps: taking the number one as a starting mark for electronic eye capture; the electronic eye detects whether the mark of the position corresponding to the mapping azimuth is a preset number, if yes, the control device controls the first driving device to stop driving, and if not, the control device controls the first driving device to execute rotating action so that the hole position corresponding to the number rotates to the mapping azimuth.
Further, for the step of controlling the first driving device to perform the rotation action and rotating the hole site with the corresponding number to the mapping direction in the second embodiment, the second embodiment provides an implementation manner, where the implementation manner includes: acquiring the number information of the last hole site through a control device, and calculating the number information of the next hole site according to the number information of the last hole site; according to the calculated serial number information of the next hole site, the control device controls the first driving device to execute rotation action, so that the hole site with the serial number corresponding to the serial number information of the next hole site rotates to the mapping azimuth.
Embodiment III:
the third embodiment provides a sampling system of a disc-type environment DNA filter cake replacement device, the sampling system comprises the sampling method of any one of the disc-type environment DNA filter cake replacement device, the method achieves the effect of filter cake alternate replacement, and the technical problem of replacement errors caused by different methods in the prior art by manually replacing a filter membrane is solved.
Since the second embodiment, the third embodiment and the first embodiment are one embodiment of the same inventive concept and have the same partial structures, the structures of the second embodiment, the third embodiment and the first embodiment that are substantially the same are not described in detail, and the detailed description of the second embodiment is omitted.
Finally, it should be noted that: the above examples are only specific embodiments of the present invention, and are not intended to limit the scope of the present invention, but it should be understood by those skilled in the art that the present invention is not limited thereto, and that the present invention is described in detail with reference to the above examples: any person skilled in the art may modify or easily conceive of the technical solution described in the foregoing embodiments, or perform equivalent substitution of some of the technical features, while remaining within the technical scope of the present disclosure; such modifications, changes or substitutions do not depart from the spirit of the corresponding technical solutions. Are intended to be encompassed within the scope of the present invention. Therefore, the protection scope of the invention is subject to the protection scope of the claims.
Although embodiments of the present invention have been disclosed above, it is not limited to the details and embodiments shown and described, it is well suited to various fields of use for which the invention would be readily apparent to those skilled in the art, and accordingly, the invention is not limited to the specific details and illustrations shown and described herein, without departing from the general concepts defined in the claims and their equivalents.
Claims (6)
1. A sampling method of a disk-type environmental DNA filter cake replacement device, the filter cake being assembled as an integrally structured filter cake by an annular housing, the replacement device comprising: the device comprises a magazine mechanism, a sampling area, a rotary table, a detection device and a control device, wherein the rotary table is disc-shaped and is driven to rotate by a first driving device arranged at the center of the rotary table, and a first hole, a second hole, a third hole and a fourth hole which are matched with the filter cake are formed in the rotary table; the control device is correspondingly connected with the detection device and the first driving device; the magazine mechanism and the sampling area are correspondingly arranged on paths of the first hole, the second hole, the third hole and the fourth hole which rotate around the circle center; a plurality of filter cakes are arranged in the magazine mechanism in an up-down overlapping way, and a channel which just passes through one filter cake is reserved at the bottom of the magazine mechanism; the sampling area is used for collecting a water sample of the filter cake; the sampling method is characterized by comprising the following steps:
executing N times of rotation actions through the first driving device, wherein N is greater than or equal to 4;
when the first driving device executes a first rotation action, the first hole is rotated to a first position to load a filter cake;
when the first driving device executes a second rotation action, the first hole is rotated to a second position, water sample collection is performed, and the second hole is rotated to the first position;
when the first driving device executes a third rotation action, the first hole is rotated to a third position, filter cake unloading is carried out, the second hole is rotated to the second position, and the third hole is rotated to the first position;
when the first driving device executes a fourth rotation action, the first hole is rotated to a fourth position to perform a pre-loading action, and the second hole is rotated to the third position, the third hole is rotated to the second position, and the fourth hole is rotated to the first position; the performing the preload action includes: cleaning and sterilizing by using a sterilizing liquid;
the first position is a through hole position of the magazine type mechanism, and the second position is a position of the sampling area;
the sampling method further comprises the following steps:
detecting positions of the first hole, the second hole, the third hole and the fourth hole by the detection device, and generating position information; the detecting, by the detection device, the positions of the first hole, the second hole, the third hole, and the fourth hole includes: detecting the positions of the first hole, the second hole, the third hole and the fourth hole through an electronic eye;
and acquiring the position information through the control device, judging whether the first hole, the second hole, the third hole and the fourth hole reach preset positions according to the position information, if so, controlling the first driving device to stop executing the rotation action, and if not, controlling the first driving device to continue executing the rotation action.
2. The method of claim 1, wherein rotating the first hole to a second position when the first driving device performs a second rotation, and performing water sample collection comprises:
when the first driving device executes the second rotation action, the first hole is rotated to the second position, and water sample collection is performed for preset time.
3. The method of claim 1, wherein when the first driving device performs a second rotation, the first hole is rotated to a second position, and after water sample collection, the method further comprises:
and detecting the water sample acquisition result, stopping the water sample acquisition if the result reaches a preset detection value, and continuing to acquire the water sample if the result does not reach the preset detection value.
4. The sampling method of the disc-type environmental DNA filter cake replacement device according to claim 1, wherein:
the electronic grabbing position of the electronic eye is fixedly mapped on one of the positions of the turntable, and before the positions of the first hole, the second hole, the third hole and the fourth hole are detected by the electronic eye, the sampling method further comprises:
a plurality of marks which are in one-to-one correspondence with the first hole, the second hole, the third hole and the fourth hole and can be identified by the electronic eyes are arranged on the turntable, and one of the marks is used as a starting mark for capturing by the electronic eyes;
the detecting, by the electronic eye, the positions of the first hole, the second hole, the third hole, and the fourth hole includes:
detecting whether the mark exists at the position corresponding to the mapping azimuth through the electronic eye, and generating mark information;
the step of obtaining the position information through the control device, judging whether the first hole, the second hole, the third hole and the fourth hole reach a preset position according to the position information, if yes, controlling the first driving device to stop executing the rotation action, and if not, controlling the first driving device to continue executing the rotation action comprises the following steps:
and acquiring the marking information by the control device, judging whether the first hole, the second hole, the third hole and the fourth hole reach preset positions according to the marking information, if so, controlling the first driving device to stop executing preset rotation actions, and if not, controlling the first driving device to continue executing the rotation actions.
5. The method for sampling a disc-type environmental DNA cake replacement device according to claim 4, wherein after the plurality of marks which are one-to-one corresponding to the first hole, the second hole, the third hole, and the fourth hole and are recognized by an electronic eye are provided on the turntable, the method for sampling further comprises:
numbering the marks, enabling the first holes to correspond to a first number, enabling the second holes to correspond to a second number, enabling the third holes to correspond to a third number and enabling the fourth holes to correspond to a fourth number;
taking one of the marks as a starting mark for electronic eye capturing;
the detecting, by the electronic eye, the positions of the first hole, the second hole, the third hole, and the fourth hole includes:
detecting whether the mark exists at the position corresponding to the mapping azimuth through the electronic eye; if yes, the control device controls the first driving device to execute a preset rotation action, and if not, the control device controls the first driving device to stop rotating, wherein the control device comprises the following steps:
taking the number one as a starting mark for the electronic eye capture;
and detecting whether the mark of the position corresponding to the mapping azimuth is a preset number or not through the electronic eye, if so, controlling the first driving device to stop driving by the control device, and otherwise, controlling the first driving device to execute the rotating action by the control device so as to enable the hole position of the corresponding number to rotate to the mapping azimuth.
6. The method for sampling a disc-type environmental DNA filter cake changer of claim 5, wherein,
controlling the first driving device to execute the rotation action, and enabling the hole positions with corresponding numbers to rotate to the mapping azimuth comprises the following steps:
acquiring the number information of the last hole site through the control device, and calculating the number information of the next hole site according to the number information of the last hole site;
and controlling the first driving device to execute the rotating action through the control device according to the calculated serial number information of the next hole site, so that the hole site with the serial number corresponding to the serial number information of the next hole site rotates to the mapping azimuth.
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