CN114506930A - Application of mucor circinelloides FNZJ3-2-2 in removing hexavalent chromium in wastewater - Google Patents
Application of mucor circinelloides FNZJ3-2-2 in removing hexavalent chromium in wastewater Download PDFInfo
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Abstract
The invention discloses application of mucor circinelloides FNZJ3-2-2 in removing hexavalent chromium in wastewater, and belongs to the technical field of microorganisms. Through a single-factor test and a tolerance test, the suitable biological adsorption conditions of the strain on Cr (VI) wastewater are determined as follows: cr (VI) with the initial concentration of 30-40 mg/L, initial bacteria content of 2.0 x 10^ 8/mL-3.0 x 10^ 8/mL, initial pH of 7.0-8.0, adsorption time of 28-32 h, adsorption temperature of 33-34 ℃ and rotation speed of 160 r/min. When the optimized adsorption condition reaches the balance, the removal rate of the FNZJ3-2-2 strain to Cr (VI) and total chromium in Cr (VI) wastewater reaches 90.5 percent and 64.8 percent respectively.Mucor circinelloides The FNZJ3-2-2 strain has strong adsorption-reducibility, tolerance and resistance to Cr (VI) in Cr (VI) wastewater, and the action ranges of the FNZJ3-2-2 strain are respectively 0-60 mg/L, 60-100 mg/L and more than or equal to 100mg/L of initial Cr (VI).
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to an application of mucor circinelloides FNZJ3-2-2 in removing hexavalent chromium in wastewater.
Background
Heavy metal compounds are widely used in industries such as metal processing, electroplating, electronics, mining operations, fertilizer production, battery manufacturing, tanneries, and the like, and are responsible for the direct or indirect emission of heavy metals into the environment. Waste residues such As sewage (waste water) polluted by heavy metals (Cr, Cu, Ni, Co, Pb, Cd, Hg, Ag) and metalloid (As) and electroplating sludge rich in heavy metals have persistent biological toxicity and cause serious harm to human health and environment, and attract great attention, wherein Cr generally has two oxidation states of Cr (VI) (Cr (VI)) and trivalent chromium (Cr (III)), and in organisms, Cr (VI) generally has higher chemical toxicity than Cr (III). Therefore, the item takes Cr as a representative to research the hazard of heavy metal pollution.
The treatment of heavy metal pollution by microbial adsorption is one of the research hotspots in recent years, and the biological process of heavy metal pollution treatment can comprise adsorption, accumulation and enrichment mechanisms, such as bioadsorption and bioflocculation of microbial metabolites. Clostridium bacterial strain Lysi separated from chromium (Cr) polluted metal electroplating wastewater by ozzle and the like of Huazhong agriculture universityThe complete genome information of the nibacillus fusiformis ZC1 shows that the strain has the capability of reducing toxic Cr (VI) into trivalent chromium Cr (III) with lower toxicity. A Bacillus cereus (Bacillus cereus WTxJ1-4, CGMCC number 10053, Genbank No. KP150574, ZL 201410853541.9, 202010776420.4) is provided from the screening and application of Bacillus cereus, which is a typical bacterium in the paper-making wastewater sewage irrigation reed field, such as the Lichoxia of the salt city institute and the like, and can preferentially degrade COD in the paper-making wastewater sewage irrigation reed fieldCrAnd AOX, exhibiting good organochlorine contaminant removal capacity and strong cr (vi) adsorption-reduction capacity.
Mucor circinelloides FNZJ3-2-2(CGMCC No.9684) is 1 dominant nickel-rich patent strain (patent ZL 201510293835.5) screened from the waste liquid of a plating tank in the early stage of the laboratory. Mucor circinelloides FNZJ3-2-2 has Ni (II) adsorption (enrichment) function, but no research has shown that the strain has the possibility of treating Cr (VI) contamination or Cr (VI) and Ni (II) synergistic contamination, and how the strain is applied to remove Cr (VI) and the bioadsorption, tolerance and resistance thereof are unknown. Therefore, the present invention focuses on the study of the adsorption-reduction ability of Mucor circinelloides FNZJ3-2-2 to Cr (VI).
Disclosure of Invention
The technical problem to be solved is as follows: aiming at the technical problems, the invention provides an application of Mucor circinelloides FNZJ3-2-2 in removing hexavalent chromium in wastewater, the strain finds a new idea and a new method for treating Cr (VI) pollution or Cr (VI) and Ni (II) synergistic pollution in Cr (VI) wastewater, and the removal rates of Cr (VI) and total chromium in Cr (VI) wastewater by using a bacterial suspension respectively reach 90.5% and 64.8%.
The technical scheme is as follows: application of mucor circinelloides FNZJ3-2-2 in removing hexavalent chromium in wastewater.
Preferably, the application is: adding the FNZJ3-2-2 strain suspension growing in the logarithmic phase into the wastewater to ensure that the initial strain content of the wastewater is 0.5 x 10^ 8/mL-4.0 x 10^ 8/mL, and culturing for 24-36 h under the conditions of pH3.0-pH8.0, adsorption temperature of 30-34 ℃ and rotation speed of 160 r/min.
Preferably, the bacterial suspension comprises LB liquid culture medium, and the components of the LB liquid culture medium are 10g/L of peptone, 5g/L of yeast extract and 10g/L of sodium chloride.
Preferably, the initial bacteria content is 2.0 x 10^8 to 3.0 x 10^ 8/mL.
Preferably, the concentration of hexavalent chromium in the wastewater is not higher than 100 mg/L.
Preferably, the pH value of the application is 7.0-8.0.
Preferably, the adsorption temperature is 33-34 ℃.
Preferably, the culture time is 28-32 h.
Has the advantages that: single factor experiments prove that the Mucor circinelloides FNZJ3-2-2 bacterial suspension has extremely strong adsorption and reduction effects on Cr (VI) in Cr (VI) wastewater. Under the optimized condition, the removal rate of the Mucor circinelloides FNZJ3-2-2 bacterial suspension to Cr (VI) and total chromium in Cr (VI) wastewater respectively reaches 90.5 percent and 64.8 percent. The strain has extremely strong potential for treating Cr (VI) pollution or Cr (VI) and Ni (II) synergistic pollution. The mucor circinelloides also has strong adsorption reducibility, tolerance and resistance to Cr (VI) pollution, and is mainly expressed as bioadsorption-reducibility when the initial Cr (VI) concentration is 0-60 mg/L; when the initial Cr (VI) concentration is 60-100 mg/L, the Cr (VI) mainly shows tolerance; at initial Cr (VI) concentrations of 100mg/L or more, resistance or lethality is mainly manifested.
Drawings
FIG. 1 change of Cr (VI) removal rate of FNZJ3-2-2 strain with adsorption time at different initial Cr (VI) concentrations;
FIG. 2 Cr (VI) removal rate as a function of adsorption time for different initial FNZJ 3-2-bacteria contents in the wastewater;
FIG. 3 change of Cr (VI) removal rate of FNZJ3-2-2 strain at different adsorption pH with adsorption time;
FIG. 4 shows the Cr (VI) removal rate of FNZJ3-2-2 strain at different adsorption temperatures as a function of adsorption time;
FIG. 5 FNZJ3-2-2 strain in the presence of suitable adsorption conditions for Cr (VI) and total chromium removal rate and bacteria content;
FIG. 6 the kinetics of Cr (VI) and total chromium adsorption of the FNZJ3-2-2 strain under suitable adsorption conditions;
FIG. 7 FNZJ3-2-2 strain shows the change of Cr (VI) biological removal rate and bacteriostasis rate under proper adsorption conditions for different initial Cr (VI) concentrations.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to the specific contents of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without making any creative effort, shall fall within the protection scope of the present invention.
Preservation of strains
The bacillus cereus is preserved in the common microorganism center of China general microbiological culture Collection center at 19 th 9 th 2014, the preservation address is No. 3 of Xilu No. 1 of Beijing Korean district, and the preservation number is CGMCC No. 9684. The separation and identification method of the strain is described in patent ZL 201510293835.5.
II, bioadsorption, tolerance and resistance of the strain to Cr (VI) in Cr (VI) wastewater
Example 1 bioadsorption-reduction test of Bacillus cereus for Cr (VI)
1. Preparation of bacterial suspension
Taking a plurality of LB culture solutions of the bacillus cereus in the logarithmic phase, centrifuging for 10min at 4 ℃ and 8000r/min, adding sterile water, and repeatedly centrifuging for 3 times to prepare freeze-dried powder. Before the test, a proper amount of freeze-dried powder is taken and dispersed in sterile physiological saline to prepare bacterial suspension with the bacterial content of 4.0 x 10^ 10/mL, and the bacterial suspension is preserved at 4 ℃ for later use.
2. Biosorption-reduction test Single factor test for Mucor circinelloides FNZJ3-2-2
0.1-1 mL of FNZJ3-2-2 bacterial suspension is respectively taken, added into 100mL of LB culture solution containing initial Cr (VI) with the concentration of 0-120 mg/L, cultured for 0-36 h under the conditions of pH4.0-pH8.0, adsorption temperature of 30-34 ℃ and rotation speed of 160r/min, and single-factor suitable conditions for removing Cr (VI) pollutants from Cr (VI) wastewater by FNZJ3-2-2 bacterial strain are explored. In the single-factor test, the preset fixed values are respectively as follows: cr (VI) wastewater contains FNZJ3-2-2 strain with initial bacteria content of 4.0 x 10^ 8/mL, initial Cr (VI) concentration of 40mg/L, adsorption pH of 7.0, adsorption temperature of 30 ℃ and adsorption speed of 160 r/min. The residual Cr (VI) and the total Cr concentration in the reaction liquid after centrifugation are respectively measured by a dibenzoyl dihydrazide spectrophotometry method, and the biological removal rate of the FNZJ3-2-2 strain to the Cr (VI) and the total Cr is calculated.
(1) Under other preset fixed values of single factors, the concentrations of the potassium dichromate are respectively changed to be 20mg/L, 30mg/L, 40mg/L, 50mg/L and 60mg/L, and the results are shown in figure 1;
(2) at a preset fixed value of the other single factor, change: cr (VI) wastewater containing FNZJ3-2-2 strain with initial bacteria content of 0.5X 10^ 8/mL, 1X 10^ 8/mL, 2X 10^ 8/mL, 3X 10^ 8/mL and 4X 10^ 8/mL, the results are shown in FIG. 2;
(3) changing the adsorption pH to pH4.0, pH5.0, pH6.0, pH7.0 and pH8.0 respectively at other single preset fixed values, the results are shown in FIG. 3;
(4) the adsorption temperatures were changed to 30 deg.C, 31 deg.C, 32 deg.C, 33 deg.C and 34 deg.C, respectively, at other single-factor preset fixed values, the results are shown in FIG. 4;
3. biological adsorption-reduction kinetics of FNZJ3-2-2 strain on Cr (VI) wastewater under optimal conditions
Under the conditions of optimal adsorption condition pH7.0, initial Cr (VI) concentration of 40mg/L, 33 ℃, 160r/min and adsorption time of 28h of the FNZJ3-2-2 strain to Cr (VI), sampling at different times to determine the total Cr (VI) concentration in the reaction solution without blank and before and after centrifugation, calculating corresponding removal rates and obtaining a corresponding adsorption kinetic equation, wherein the results are shown in figure 5, figure 6 and table 1.
TABLE 1 distribution of total Cr, Cr (VI) and Cr (III) in reaction solution and cells after Cr (VI) removal from wastewater under optimum conditions
FIGS. 5 and 6 show that under the optimal conditions, the FNZJ3-2-2 strain shows that the curves of the removal rate of Cr (VI) and total chromium in Cr (VI) wastewater show that the curves of the removal rate of Cr (VI) and total chromium in the wastewater rapidly rise and then slowly fall,consistent with the change of the bacteria content in the reaction. When the reaction time reaches 28h, the removal rates of Cr (VI) and total chromium of the FNZJ3-2-2 strain in Cr (VI) wastewater are respectively 90.5% and 64.8%. The kinetic equations of bioadsorption of Cr (VI) and total chromium are respectively 0.0354x2-2.0764x +36.936 (R)20.995) and y 0.0476x2-2.5784x +36.329 (R)20.9991), a two-stage kinetic model is followed. The change curves of Cr (VI) and total chromium in the cell-free centrifugate and Cr (VI) before centrifugation are in positive correlation, the difference is rapidly increased from about 8 percent to about 92 percent along with the prolonging of the adsorption time, the solution not only has Cr (VI) remained but also generates Cr (III) after the reaction, and the biological removal condition of Cr (VI) can be reacted by monitoring Cr (VI) before or after centrifugation.
Table 1 shows that FNZJ3-2-2 strain does have a strong ability to remove Cr (VI) and reduce a part of Cr (VI) to Cr (III). Among the total chromium adsorbed by the cells, 7.1-32.5% is Cr (III), and most is Cr (VI).
Example 2 tolerance and resistance characteristics of Bacillus cereus to Cr (VI)
Initial Cr (VI) with the concentrations of 0mg/L, 20mg/L, 40mg/L, 60mg/L, 80mg/L, 100mg/LL and 120mg/L are respectively added into 100mL of LB culture medium, optimal logarithmic phase FNZJ3-2-2 strain suspension is sequentially added into the initial Cr (VI), 3 parallel strains are arranged in each concentration, random sampling is carried out at regular time, the biomass of the thallus, the residual Cr (VI) and the total Cr in the solution are measured, and time relation curves of the biomass of the thallus, the Cr (VI) removal rate and the total Cr removal rate of the FNZJ3-2-2 strain are respectively established, as shown in figure 7.
As can be seen from FIG. 7, the bacteriostatic rate of the microorganism tends to be flat after rapidly increasing with the increase of the initial Cr (VI) concentration, which indicates that Cr (VI) has a significant inhibitory effect on the growth of the microorganism, and the strain has good adsorption, tolerance and resistance to potassium dichromate due to the growth of the microorganism in a certain amount compared with the initial inoculation amount. When the initial Cr (VI) concentration is within 0-60 mg/L, the total chromium biological removal rate and the Cr (VI) biological removal rate are not changed greatly, and the FNZJ3-2-2 strain shows good adsorbability. When the initial Cr (VI) concentration is 60-100 mg/L, the biological removal rate of the total chromium and the Cr (VI) is in a gentle descending trend, and the FNZJ3-2-2 strain shows good tolerance. When the initial Cr (VI) concentration is 100-120 mg/L, the high total chromium and Cr (VI) biological removal rate is still maintained, but the bacterium content of the FNZJ3-2-2 strain is obviously reduced, so that the FNZJ3-2-2 strain shows strong resistance.
The above examples are only for illustrating the technical idea and features of the present invention, and the purpose of the present invention is to enable those skilled in the art to understand the content of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims (8)
1. Application of Mucor circinelloides FNZJ3-2-2 in removing hexavalent chromium in wastewater.
2. The application according to claim 1, wherein the application is: adding the FNZJ3-2-2 strain suspension growing in the logarithmic phase into the wastewater to ensure that the initial strain content of the wastewater is 0.5 x 10^ 8/mL-4.0 x 10^ 8/mL, and culturing for 24-36 h under the conditions of pH4.0-pH8.0, adsorption temperature of 30-34 ℃ and rotation speed of 160 r/min.
3. The use according to claim 2, wherein the bacterial suspension comprises LB liquid medium, the composition of which is 10g/L peptone, 5g/L yeast extract and 10g/L sodium chloride.
4. The use of claim 2, wherein the initial germ content is 2.0 x 10^8 to 3.0 x 10^ 8/mL.
5. Use according to claim 2, characterized in that the concentration of hexavalent chromium in said effluents is not higher than 100 mg/L.
6. The use according to claim 2, wherein the pH of the use is 7.0 to 8.0.
7. Use according to claim 2, wherein the adsorption temperature is 33 to 34 ℃.
8. The use according to claim 2, wherein the incubation time is 28-32 h.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116355763A (en) * | 2023-05-26 | 2023-06-30 | 云南省林业和草原科学院 | Symbiotic fungus for oil wheat and spruce and application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103525870A (en) * | 2013-10-23 | 2014-01-22 | 嘉兴学院 | Microbial flocculant as well as preparation method and application thereof |
| JP2015120119A (en) * | 2013-12-24 | 2015-07-02 | 株式会社トーワ建設 | Environment improvement method using microorganisms reducing hexavalent chromium |
| CN104830702A (en) * | 2015-06-01 | 2015-08-12 | 盐城工学院 | Mucor circinelloides FNZJ3-2-2 resistant to aluminum profile electronickelling bath solution and application of mucor circinelloides FNZJ3-2-2 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103525870A (en) * | 2013-10-23 | 2014-01-22 | 嘉兴学院 | Microbial flocculant as well as preparation method and application thereof |
| JP2015120119A (en) * | 2013-12-24 | 2015-07-02 | 株式会社トーワ建設 | Environment improvement method using microorganisms reducing hexavalent chromium |
| CN104830702A (en) * | 2015-06-01 | 2015-08-12 | 盐城工学院 | Mucor circinelloides FNZJ3-2-2 resistant to aluminum profile electronickelling bath solution and application of mucor circinelloides FNZJ3-2-2 |
Non-Patent Citations (1)
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| J.M.TOBIN ET AL.: "Mucor biosorbent for chromium removal from tanning effluent", 《WATER RESEARCH》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116355763A (en) * | 2023-05-26 | 2023-06-30 | 云南省林业和草原科学院 | Symbiotic fungus for oil wheat and spruce and application thereof |
| CN116355763B (en) * | 2023-05-26 | 2023-08-29 | 云南省林业和草原科学院 | Symbiotic fungus for oil wheat and spruce and application thereof |
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