CN114354602B - Egg food safety in production uses biochemical check out test set with early warning suggestion - Google Patents

Abstract

The invention discloses biochemical detection equipment with an early warning prompt for egg food safety production, which comprises a detection table and a placement frame, wherein the placement frame is installed at the top of the detection table, three groups of sample observation frames are placed at the top of the detection table, a transparent cover plate is installed on the inner wall of a groove through a shaft rod, a first liquid storage groove is formed in the bottom wall of the groove, a background lamp is installed in the sample observation frames in an embedded mode, a second liquid storage groove is formed in the top of the sample observation frames, and an alarm lamp is installed at the top of the sample observation frames; and a culture test tube is embedded in the limiting hole. According to the invention, the sample observation frame is arranged, the sample liquid in the first liquid storage tank is observed in form under the assistance of the background light by using the photoelectric microscope, the pH value of the sample liquid in the second liquid storage tank is detected by using the pH detector, and when the sample in the sample observation frame is detected to be acidic, the alarm lamp gives an alarm to prompt a detector to remind the detector that salmonella exists in the sample.

Description

Egg food safety in production uses biochemical check out test set with early warning suggestion

Technical Field

The invention relates to the technical field of biochemical detection, in particular to biochemical detection equipment with early warning prompt for egg food safety production.

Background

Eggs contain a large amount of vitamins, minerals and high-biological-value proteins, and are one of foods which are usually eaten by human beings, but because the eggs and chicken manure are discharged from the same outlet, salmonella is very easily stained on the surfaces of the eggs, and the eating safety of the eggs is damaged to a certain extent, so that when the eggs are used for food processing, a food sample needs to be subjected to biochemical detection to judge whether the salmonella is carried or not, and the food safety is ensured.

The existing biochemical detection equipment has the following defects:

1. patent document CN209043906U discloses a biochemical detection device, "including a detection table, on which a light source device, a reaction device and a detection device are arranged, the light source device, the reaction device and the detection device are sequentially arranged on the detection table from left to right, a light source and a light source channel are arranged in the light source device, an optical fiber collimating lens, a filter and a focusing lens are sequentially arranged in the light source channel, the light source device provides a light source, the reaction device and the detection device are sequentially located on an emergent light path behind the light source and are all placed perpendicular to an optical axis, light emitted by the light source is irradiated on the reaction device, the detection device is located behind the reaction device, the detection device detects light passing through the reaction device, and transmits collected data information to a data processing system for data processing; the utility model adopts the combination of the light source device and the detection device to detect the sample in the reaction cup, the automation degree is high, the efficiency is high, and the lifting adjusting device adjusts the height of the reaction cup to meet the detection of different reagents or samples, the detection device can not realize the synchronous detection in the aspects of optics and chemistry when in use, and further can not send out early warning prompt in time after the detection is finished;

2. patent document CN214374847U discloses a medical blood biochemical detection device, "including an operation panel, the operation panel outside from a left side to the right side connected in turn hemoglobin detection platform, leukocyte detection platform, platelet detection platform, neutrophil detection platform, lymphocyte detection platform, the operation panel inside is equipped with a conveyor belt, the right side inside the conveyor belt is connected with a right roller, the right roller outside is connected with a right support frame, and the right support frame bottom is fixedly connected to the inner wall of the operation panel, the right roller outside is connected with a belt, the belt left side is connected to the right roller, and the belt right side is connected with a motor, the medical blood biochemical detection device, blood biochemical detection becomes pipeline work, multiple working personnel respectively master the corresponding detection platform, finally gather and record in case, can save time and efficiently complete blood biochemical detection task, the problems that a plurality of medical workers run from east to west and are laboured hard for blood detection are solved, and the biochemical detection equipment cannot heat a sample to further remove the structure of a target flora when in use, so that the detection operation of the detection equipment is not convenient and fast;

3. patent document CN210894369U discloses a biochemical detection apparatus, "comprising a substrate and provided thereon: the liquid channel comprises a liquid inlet channel and a reaction channel which are communicated with each other; a calibration channel in communication with said reaction channel through which a calibration reagent can flow into said reaction channel; a gas channel which is communicated with the liquid inlet channel, wherein gas can drive liquid in the liquid inlet channel to flow to the reaction channel through the gas channel; and the reaction electrode on the detection test paper extends into the reaction channel. The utility model has the advantages that the mutually communicated gas channel and liquid channel are arranged, so that a sample can conveniently flow from the liquid inlet channel to the reaction channel under the pushing action of gas, the sample is driven to flow in a pneumatic mode, the operation is simple and convenient, meanwhile, the manufacturing process of biochemical detection equipment is simplified, the cost is reduced, the biochemical detection equipment cannot detect the internal oxygen environment when in use, and the result accuracy in the subsequent comparison detection is poor;

4. patent document CN108717113A discloses a biochemical detector, "which comprises a frame, a bottom plate, a test tube rack, a tube rack logistics board, a sample introduction driving device, a positioning driving device, an optical detection device, a sampling device, a cleaning device and a sample discharge driving device, wherein the bottom plate and the sampling device are fixedly arranged on the frame, the tube rack logistics board is arranged above the bottom plate through a connecting column, and the sample introduction driving device, the positioning driving device and the sample discharge driving device are fixedly arranged on the bottom plate; this detector has realized the automatic business turn over material of test-tube rack to the automated conveying detects to the detection station, has realized automatic, quick, convenient detection, can satisfy the demand that detects in batches, and this biochemical detector only improves to the storage of advancing of test-tube rack when using, fails to adjust to the test-tube rack internal culture environment, makes the result comprehensiveness that biochemical detection is not enough.

Disclosure of Invention

The invention aims to provide biochemical detection equipment with an early warning prompt for egg food safety production, and aims to solve the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: the biochemical detection equipment with the early warning prompt function for egg food safety production comprises a detection table and a placement frame, wherein the placement frame is installed at the top of the detection table, three groups of sample observation frames which are arranged at equal intervals are placed at the top of the detection table, and the sample observation frames are positioned in front of the placement frame;

the liquid storage device comprises a groove at the top of a sample observation frame, wherein a transparent cover plate is installed on the inner wall of the groove through a shaft rod, a first liquid storage tank is arranged on the bottom wall of the groove, a background lamp is installed in the sample observation frame in an embedded mode and is positioned below the first liquid storage tank, a second liquid storage tank is arranged at the top of the sample observation frame and is positioned on one side of the first liquid storage tank, and an alarm lamp is installed at the top of the sample observation frame and is positioned in front of the second liquid storage tank;

the top of rack is equipped with spacing hole, the inside gomphosis in spacing hole is installed and is cultivateed the test tube.

Preferably, the diapire of rack is equipped with the heating groove, the diapire of heating groove is installed and is heated electric net piece, the asbestos gauge is installed at the top of heating electric net piece, and the top of asbestos gauge and the bottom contact of cultivateing the test tube, the time-recorder that the equidistance was arranged is installed to the diapire of rack, and time-recorder and heating groove interval arrangement, time-recorder and heating electric wire netting piece electric connection.

Preferably, the bottle plug is installed in the top gomphosis of cultivateing the test tube, the inside of bottle plug runs through and installs the intake pipe, the inner wall installation oxygen sensor of intake pipe, the surface mounting of intake pipe has the display screen, and display screen and oxygen sensor electric connection, the inside of bottle plug runs through and installs the sample straw, and the sample straw is located one side of intake pipe.

Preferably, the inside seal of intake pipe installs the cladding pipe, the internally mounted of cladding pipe has oxygen air feed pipe and nitrogen gas feed pipe, and the nitrogen gas feed pipe is located one side of oxygen gas feed pipe, the tail end of nitrogen gas feed pipe extends to cultivates the inside lower extreme of test tube, the internally mounted who detects the platform has the gas receiver, the internally mounted of gas receiver has the baffle, the baffle becomes nitrogen gas chamber and oxygen chamber with the inside of gas receiver, and the oxygen chamber is located one side of nitrogen gas chamber, the one end of nitrogen gas feed pipe is run through and is detected the inside that the platform extended to nitrogen gas chamber, one degree of eyes of oxygen gas feed pipe is run through and is detected the inside that the platform extended to oxygen gas chamber.

Preferably, the surfaces of the oxygen gas supply pipe and the nitrogen gas supply pipe are respectively provided with an electronic valve, the insides of the oxygen gas supply pipe and the nitrogen gas supply pipe are respectively provided with a one-way valve, and the direction of the one-way valve inside the oxygen gas supply pipe inside the middle group of the culture test tubes is opposite to that of the nitrogen gas supply pipe.

Preferably, the inside of detecting the platform is installed with clean cask, and clean cask is located one side of gas receiver, the suction pump is installed in the front of clean cask, the input and the clean cask of suction pump lead to pipe and be connected, it washs tap to install at the top of detecting the platform, and washs tap and be located the side the place ahead of sample observation frame, the top of detecting the platform is equipped with the wash basin, and washs the place ahead that the basin is located washing tap, the output and the washing tap of suction pump lead to pipe and be connected.

Preferably, the internally mounted who detects the platform has the photoelectric microscope case, and the photoelectric microscope case is located the place ahead of gas receiver, the slide rail is installed at the top of detecting the platform, and the slide rail is located the centre of rack and sample observation frame, the front of detecting the platform installs the dodge gate through the hinge.

Preferably, the back connection who examines the test table has the mounting bracket, the carousel is installed at the top of mounting bracket, the telescopic link is installed at the top of carousel, the extension platform is installed at the top of telescopic link, driving motor is installed at the top of extension platform, driving motor's output runs through the extension platform and installs the puddler, examine the top of test table and install the churn, and the churn is located one side of rack, the surface encirclement of puddler installs cutting blade.

Preferably, a storage box is installed at the top of the sample observation rack, an acid-base detector is placed in the storage box, and the acid-base detector is electrically connected with the alarm lamp.

Preferably, the working steps of the detection device are as follows:

s1, before biochemical detection operation is carried out on related samples of egg food safety production by using the detection equipment, the samples are placed into a mixing drum, then a driving motor is started, then a mixing rod is driven to cut and stir the samples in the mixing drum, then the cut samples are added into three groups of culture test tubes filled with agar culture media in equal amount, then a bottle stopper is covered, and the three groups of culture test tubes are numbered according to the left-to-right direction and are respectively a No. 1 bottle, a No. 2 bottle and a No. 3 bottle;

s2, starting the electronic valve on the surface of the oxygen gas feed pipe in the No. 1 bottle, injecting high-content oxygen into the bottle, closing the electronic valve on the surface of the nitrogen gas feed pipe to ensure that the No. 1 bottle contains oxygen with the concentration of more than 10 percent, simultaneously opening an oxygen gas supply pipe in the No. 2 bottle and an electronic valve on the surface of the nitrogen gas supply pipe, conveying nitrogen gas to the interior of the No. 2 bottle through the nitrogen gas supply pipe so as to extrude and float oxygen in the No. 2 bottle, so that the oxygen gas is transferred to the interior of an oxygen gas cavity through the oxygen gas supply pipe, so that the interior of the No. 2 bottle is in an oxygen-free environment, and the electronic valve on the surface of the oxygen supply pipe is opened for the No. 3 bottle to supply oxygen with the oxygen concentration of 4% -8% to the interior of the No. 3 bottle, further making the oxygen concentration in the No. 1 bottle, the No. 2 bottle and the No. 3 bottle in a high oxygen, oxygen-free and proper range to form a control group for subsequent culture and detection operation;

s3, after 24-36 h of culture, equivalently sampling samples in the No. 1 bottle, the No. 2 bottle and the No. 3 bottle by using a sampling suction pipe, putting the samples into a first liquid storage tank and a second liquid storage tank, immediately turning over a transparent cover plate to cover the top of the first liquid storage tank, taking out a photoelectric microscope in a photoelectric microscope box, embedding and installing the photoelectric microscope on the surface of a slide rail, observing the sample liquid in the first liquid storage tank with a background lamp by using the photoelectric microscope in a form, judging whether salmonella exists or not, detecting the pH value of the sample liquid in the second liquid storage tank by using an acid-base detector, and judging whether salmonella exists or not by combining optical observation of the photoelectric microscope if the pH value of the sample liquid in the second liquid storage tank is acidic;

s4, in order to reduce the interference of morphologically less-different-appearance pathogenic bacteria on the optical identification result of the salmonella, starting a heating electric mesh, heating the bottle No. 1, the bottle No. 2 and the bottle No. 3 placed in the placing rack, setting the heating time for 4 minutes through a timer, repeating S3, comparing the heating time with the pH value after the first S3 detection, judging that the salmonella is contained if the pH value is unchanged, otherwise, detecting other high-temperature-resistant pathogenic bacteria and determining the strains;

s5, after the detection is finished, the photoelectric microscope is placed back into the photoelectric microscope box, then the sample liquid in the first liquid storage tank and the second liquid storage tank on the surface of the sample observation frame is poured, then the sample liquid is placed into the cleaning basin, the suction pump is started, and the clean water in the cleaning water bucket is pumped to the cleaning water faucet for cleaning.

Compared with the prior art, the invention has the beneficial effects that:

1. the invention is provided with a sample observation frame, sample liquid is respectively sampled and put into the first liquid storage tank and the second liquid storage tank, a photoelectric microscope moves along the surface of a slide rail and is used for observing the shape of the sample liquid in the first liquid storage tank under the assistance of a background lamp, so as to preliminarily judge whether salmonella exists, then an acid-base detector is used for detecting the acid-base value of the sample liquid in the second liquid storage tank, and when the sample in the sample observation frame is detected to be acidic, an alarm lamp is started to send an alarm prompt to remind a detector that salmonella exists in the sample.

2. The salmonella detection device is provided with a placing frame, a timer, a heating groove and a heating electric net piece, in order to reduce the interference of germs with small shape difference on the optical identification result of salmonella morphologically, the heating electric net piece is started, then a number bottle, a number bottle and a number bottle placed in the placing frame are heated, the heating time is set for minutes by the timer, S is repeated, the S is compared with the pH value after the first S detection, if the pH value is not changed, the salmonella is judged to be contained, otherwise, other high-temperature resistant germs are required to be detected additionally to determine the strains.

3. The oxygen concentration in the culture test tube can be detected through the oxygen sensor by installing the culture test tube and the oxygen sensor, so that a necessary monitoring basis is provided for the subsequent oxygen supply and discharge operation, and the oxygen concentration is visually displayed through the display screen so as to be conveniently checked by a detection person.

4. The oxygen gas supply pipe and the nitrogen gas supply pipe are arranged, and the oxygen content in the three groups of culture test tubes is adjusted, so that the oxygen concentration in the number bottle, the number bottle and the number bottle is in a high oxygen, anaerobic and appropriate range, and a control group is formed for subsequent culture detection operation.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic view of the structure at A in FIG. 1 according to the present invention;

fig. 3 is a schematic view of the mounting structure of the rack of the present invention;

FIG. 4 is a schematic view of the installation structure of the asbestos gauge and the heating grid sheet of the present invention;

FIG. 5 is a schematic view of the sample viewing rack and storage box mounting arrangement of the present invention;

FIG. 6 is a schematic view of the bottle stopper and oxygen sensor mounting structure of the present invention;

FIG. 7 is a schematic view of the installation structure of the bottle stopper, the air inlet pipe and the cladding pipe of the present invention;

fig. 8 is a schematic view of the internal structure of the air cylinder of the present invention.

In the figure: 1. a detection table; 101. cleaning a faucet; 102. a wash basin; 2. a movable door; 3. a mounting frame; 301. a telescopic rod; 302. an extension platform; 303. a drive motor; 304. a stirring rod; 305. a mixing drum; 4. placing a rack; 401. a limiting hole; 402. a timer; 403. an asbestos web; 404. a heating tank; 405. heating the mesh sheet; 5. culturing the test tube; 501. a bottle stopper; 502. a display screen; 503. a sampling straw; 504. an air inlet pipe; 505. an oxygen sensor; 6. a slide rail; 7. a sample observation rack; 701. a background light; 702. a first liquid storage tank; 703. a transparent cover plate; 704. an alarm lamp; 705. a second liquid storage tank; 8. a cladding tube; 801. an oxygen supply pipe; 802. a nitrogen gas feed pipe; 803. a nitrogen chamber; 804. an oxygen chamber; 805. an air cylinder; 9. cleaning the water bucket; 10. a suction pump; 11. a photoelectric microscope box; 12. a storage box; 1201. and an acid-base detector.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front", "rear", "both ends", "one end", "the other end", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, such as "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Example one

Referring to fig. 1 and 5, an embodiment of the present invention: a biochemical detection device with early warning prompt for egg food safety production comprises a detection table 1 and sample observation frames 7, wherein three groups of sample observation frames 7 which are arranged at equal intervals are placed at the top of the detection table 1, and the sample observation frames 7 are positioned in front of a placing frame 4;

the top of the sample observation frame 7 is provided with a groove, the inner wall of the groove is provided with a transparent cover plate 703 through a shaft rod, the bottom wall of the groove is provided with a first liquid storage tank 702, the inside of the sample observation frame 7 is embedded with a background lamp 701, the background lamp 701 is positioned below the first liquid storage tank 702, the top of the sample observation frame 7 is provided with a second liquid storage tank 705, the second liquid storage tank 705 is positioned at one side of the first liquid storage tank 702, the top of the sample observation frame 7 is provided with an alarm lamp 704, and the alarm lamp 704 is positioned in front of the second liquid storage tank 705;

the top of the sample observation frame 7 is provided with a storage box 12, the acid-base detector 1201 is placed in the storage box 12, and the acid-base detector 1201 is electrically connected with the alarm lamp 704.

The inside of examining test table 1 installs the photoelectric microscope case 11, and the photoelectric microscope case 11 is located the place ahead of gas receiver 805, examines the top of test table 1 and installs slide rail 6, and slide rail 6 is located the centre of rack 4 and sample observation frame 7, examines the front of test table 1 and installs dodge gate 2 through the hinge.

Specifically, after a period of culture, the sample liquid in the three groups of culture test tubes 5 is respectively sampled and put into the first liquid storage tank 702 and the second liquid storage tank 705, and then the transparent cover plate 703 is turned over, and the top of the first liquid storage tank 702 is covered, so that the sample liquid in the first liquid storage tank 702 is isolated from being polluted by external oxygen and other foreign matters;

then the movable door 2 is opened, the photoelectric microscope in the photoelectric microscope box 11 is taken out and embedded on the surface of the slide rail 6, the sample liquid in the three first liquid storage tanks 702 can be respectively and optically checked by moving along the surface of the slide rail 6, under the color contrast difference of the background lamp 701, the visual convenience is provided for the detection personnel to better check the optical form of the flora in the sample liquid, so that the sample liquid in the first liquid storage tank 702 is subjected to form observation, whether salmonella exists or not is preliminarily judged, then the acid-base value of the sample liquid in the second liquid storage tank 705 is detected by using the acid-base detector 1201, and as the salmonella produces acid and gas in the survival process, if the acid-base value of the sample liquid in the second liquid storage tank is acidic, the acid-base value of the sample liquid in the second liquid storage tank is combined with the optical observation of the photoelectric microscope, whether the salmonella exists in the sample liquid 705 can be preliminarily judged, and if the salmonella is non-acidic, the possibility of the existence of salmonella can be preliminarily eliminated;

when the sample in the sample observation rack 7 is detected to be acidic, the alarm lamp 704 is started, and then an alarm prompt is sent out to remind a detector that salmonella exists in the sample.

Example two

Referring to fig. 1, fig. 3 and fig. 4, an embodiment of the present invention: the utility model provides an egg food safety in production uses biochemical check out test set with early warning suggestion, including examining test table 1 and rack 4, examine the top of test table 1 and install rack 4, the diapire of rack 4 is equipped with heating bath 404, heating electric wire net piece 405 is installed to the diapire of heating bath 404, asbestos gauge 403 is installed at the top of heating electric wire net piece 405, and the top of asbestos gauge 403 and the bottom contact of cultivateing test tube 5, the timer 402 that the equidistance was arranged is installed to the diapire of rack 4, and timer 402 and heating bath 404 interval arrangement, timer 402 and heating electric wire net piece 405 electric connection.

Specifically, because the heat resistance of the salmonella is poor, after the salmonella is heated for a period of time, the salmonella dies and does not have reproductive activity, so that after the reproductive activity of the salmonella is lost in the sample liquid in the three groups of culture test tubes 5, the pH value in the sample liquid does not change, if the pH value still changes, the existence of high-temperature resistant strains in the sample is represented, and further detection is needed to determine the type of the flora;

the heating time of the electric net piece 405 is timed and heated by the timer 402, and the asbestos net 403 can uniformly transfer the heat of the electric net piece 405 to the bottom of the culture test tube 5 in the process, so that the culture test tube 5 is prevented from being cracked due to uneven heating in the heating process

A support location is provided for the mounting of the heating grid plate 405 and the asbestos gauge 403 by the heating bath 404.

EXAMPLE III

Referring to fig. 1 and fig. 6, an embodiment of the present invention: the utility model provides an egg food safety in production uses biochemical check out test set with early warning suggestion, including examining test table 1 and culture test tube 5, the top of rack 4 is equipped with spacing hole 401, culture test tube 5 is installed to spacing hole 401's inside gomphosis, bottle plug 501 is installed to culture test tube 5's top gomphosis, the inside of bottle plug 501 runs through and installs intake pipe 504, intake pipe 504's inner wall installation has oxygen sensor 505, the surface mounting of intake pipe 504 has display screen 502, and display screen 502 and oxygen sensor 505 electric connection, the inside of bottle plug 501 runs through and installs sample straw 503, and sample straw 503 is located one side of intake pipe 504.

Specifically, after the culture test tubes 5 installed through the limiting holes 401 are numbered in sequence, three groups of control groups are formed for culture tests, the oxygen sensor 505 can detect the oxygen concentration inside the culture test tubes 5, necessary monitoring basis is further provided for subsequent oxygen supply and discharge operations, and the monitoring basis is visually displayed through the display screen 502 so as to be conveniently checked by a detection person;

the installation of the air inlet pipe 504 can provide pipeline support for the subsequent oxygen supply and nitrogen supply, thereby conveniently adjusting the oxygen environment inside the three groups of culture test tubes 5;

the arrangement of the bottle plug 501 can enable the culture environment inside the culture test tube 5 to be in a closed state, thereby avoiding the pollution of external foreign matters and ensuring the accuracy of detection results;

the subsequent transfer of the sample inside the culture tube 5 may be provided with instrument support by the sampling pipette 503.

Example four

Referring to fig. 1, fig. 2, fig. 7 and fig. 8, an embodiment of the present invention: the utility model provides an egg food safety in production uses biochemical detection equipment with early warning suggestion, including detecting platform 1 and cladding pipe 8, the inside seal installation of intake pipe 504 has cladding pipe 8, the internally mounted of cladding pipe 8 has oxygen gas supply pipe 801 and nitrogen gas supply pipe 802, and nitrogen gas supply pipe 802 is located one side of oxygen gas supply pipe 801, the tail end of nitrogen gas supply pipe 802 extends to cultivates the inside lower extreme of test tube 5, the internally mounted of detecting platform 1 has gas receiver 805, the internally mounted of gas receiver 805 has the baffle, the baffle becomes nitrogen gas chamber 803 and oxygen chamber 804 with the inside of gas receiver 805, and oxygen chamber 804 is located one side of nitrogen gas chamber 803, the one end of nitrogen gas supply pipe 802 runs through detecting platform 1 and extends to the inside of nitrogen gas chamber 803, one degree eye of oxygen gas supply pipe 801 runs through detecting platform 1 and extends to the inside of oxygen gas chamber 804.

Electronic valves are installed on the surfaces of the oxygen gas feed pipe 801 and the nitrogen gas feed pipe 802, and check valves are installed inside the oxygen gas feed pipe 801 and the nitrogen gas feed pipe 802, wherein the direction of the check valves inside the oxygen gas feed pipe 801 inside the middle set of culture test tubes 5 is opposite to the direction of the nitrogen gas feed pipe 802.

Specifically, an electronic valve on the surface of an oxygen gas feed pipe 801 in the No. 1 bottle is started, high-content oxygen is injected into the bottle, and an electronic valve on the surface of a nitrogen gas feed pipe 802 is closed, so that the No. 1 bottle contains oxygen with the concentration of more than 10%, a high-oxygen environment is formed, and whether the growth inhibition effect of the high-oxygen content on salmonella exists is judged;

opening electronic valves on the surfaces of an oxygen gas feeding pipe 801 and a nitrogen gas feeding pipe 802 in the No. 2 bottle, feeding nitrogen gas into the No. 2 bottle through the nitrogen gas feeding pipe 802, further extruding and floating the oxygen gas in the No. 2 bottle, and transferring the oxygen gas into an oxygen gas cavity 804 through the oxygen gas feeding pipe 801 to enable the interior of the No. 2 bottle to be in an anaerobic environment so as to judge whether salmonella exists in the interior;

aiming at the No. 3 bottle, an electronic valve on the surface of an oxygen gas supply pipe 801 is opened, oxygen with the oxygen concentration within the range of 4% -8% is supplied to the No. 3 bottle, and an oxygen environment with proper concentration is formed, so that the culture of salmonella and other possible germs is facilitated;

so that the oxygen concentration in the No. 1 bottle, the No. 2 bottle and the No. 3 bottle is in a high oxygen, oxygen-free and proper range, and a control group is formed for subsequent culture and detection operation.

Oxygen is stored in the oxygen cavity 804 in the air cylinder 805, and nitrogen is stored in the nitrogen cavity 803, so that the detection equipment can provide gas support conveniently.

The inside of the detection platform 1 is provided with a cleaning water barrel 9, the cleaning water barrel 9 is positioned on one side of the air storage barrel 805, the front side of the cleaning water barrel 9 is provided with a suction pump 10, the input end of the suction pump 10 is connected with the cleaning water barrel 9 through a water pipe, the top of the detection platform 1 is provided with a cleaning water faucet 101, the cleaning water faucet 101 is positioned in the front side of the sample observation frame 7, the top of the detection platform 1 is provided with a cleaning basin 102, the cleaning basin 102 is positioned in the front of the cleaning water faucet 101, and the output end of the suction pump 10 is connected with the cleaning water faucet 101 through a water pipe.

Specifically, the sample liquid in the first liquid storage tank 702 and the second liquid storage tank 705 on the surface of the sample observation rack 7 is poured, and then is put into the cleaning basin 102, the suction pump 10 is started, and the clean water in the cleaning water tank 9 is pumped to the cleaning water faucet 101 for cleaning.

It has mounting bracket 3 to examine the back connection of platform 1, the carousel is installed at the top of mounting bracket 3, telescopic link 301 is installed at the top of carousel, extension platform 302 is installed at the top of telescopic link 301, driving motor 303 is installed at the top of extension platform 302, driving motor 303's output runs through extension platform 302 and installs puddler 304, examine the top of platform 1 and install churn 305, and churn 305 is located one side of rack 4, the surface encirclement of puddler 304 installs cutting blade.

Specifically, put into the inside of churn 305 with the sample before the operation, rotate telescopic link 301 afterwards and make extension platform 302 be in the top of churn 305, later tensile telescopic link 301 makes puddler 304 be located the inside of churn 305, start driving motor 303, drive puddler 304 then and cut the stirring with the inside sample of churn 305 and handle, and the garrulous equivalent volume of sample after will cutting adds to three groups of culture test tubes 5 that are equipped with the agar culture medium afterwards.

The working steps of the detection device are as follows:

s1, before biochemical detection operation is carried out on related samples of egg food safety production by using the detection equipment, the samples are placed in a mixing drum 305, then a driving motor 303 is started, a mixing rod 304 is driven to cut and stir the samples in the mixing drum 305, then the cut samples are added into three groups of culture test tubes 5 filled with agar culture medium in equal amount, then a bottle stopper 501 is covered, and the three groups of culture test tubes 5 are numbered from left to right, namely a No. 1 bottle, a No. 2 bottle and a No. 3 bottle;

s2, starting an electronic valve on the surface of an oxygen gas supply pipe 801 in the No. 1 bottle, injecting high-content oxygen into the bottle, closing the electronic valve on the surface of a nitrogen gas supply pipe 802, so that the interior of the No. 1 bottle contains oxygen with the concentration of more than 10%, simultaneously opening the electronic valves on the surfaces of the oxygen gas supply pipe 801 in the No. 2 bottle and the nitrogen gas supply pipe 802, conveying nitrogen gas to the interior of the No. 2 bottle through the nitrogen gas supply pipe 802, further extruding and floating the oxygen in the No. 2 bottle, transferring the oxygen gas to the interior of an oxygen gas chamber 804 through the oxygen gas supply pipe 801, so that the interior of the No. 2 bottle is in an anaerobic environment, similarly aiming at the No. 3 bottle, opening the electronic valve on the surface of the oxygen gas supply pipe 801, conveying oxygen with the oxygen concentration of 4-8% to the interior of the No. 3 bottle, further ensuring that the oxygen concentration in the interior of the No. 1 bottle, the No. 2 bottle and the No. 3 bottle is in a high oxygen, anaerobic and proper range, forming a control group for subsequent culture detection operation;

s3, after 24-36 h of culture, equivalently sampling samples in the No. 1 bottle, the No. 2 bottle and the No. 3 bottle by using a sampling suction pipe 503 and placing the samples into the first liquid storage tank 702 and the second liquid storage tank 705, immediately turning over a transparent cover plate 703 to cover the top of the first liquid storage tank 702, taking out a photoelectric microscope in a photoelectric microscope box 11 to be embedded and installed on the surface of a slide rail 6, observing the shape of the sample liquid in the first liquid storage tank 702 with a background lamp 701 turned on by using the photoelectric microscope to judge whether salmonella exists, detecting the pH value of the sample liquid in the second liquid storage tank 705 by using an acid-base detector 1201, and judging whether salmonella exists in the sample liquid by combining optical observation of the photoelectric microscope if the pH value of the sample liquid in the second liquid storage tank 705 is acidic;

s4, in order to reduce the interference of morphologically less-different-appearance pathogenic bacteria on the optical identification result of the salmonella, starting a heating electric net 405, then heating the bottle No. 1, the bottle No. 2 and the bottle No. 3 placed in the placing rack 4, setting the heating time for 4 minutes through a timer 402, repeating S3, comparing the heating time with the pH value after the first S3 detection, if the pH value is not changed, determining that the salmonella is contained, otherwise, determining that the salmonella is other high-temperature resistant pathogenic bacteria, and needing to detect additionally to determine the strains;

s5, after the detection, the photoelectric microscope is placed back into the photoelectric microscope box 11, then the sample liquid in the first liquid storage tank 702 and the second liquid storage tank 705 on the surface of the sample observation frame 7 is poured, and then the sample liquid is placed into the cleaning basin 102, the suction pump 10 is started, and the clean water in the cleaning water bucket 9 is pumped to the cleaning water tap 101 for cleaning.

The working principle is as follows: before biochemical detection operation is carried out on samples related to egg food safety production by using the detection equipment, the samples are placed into a stirring cylinder 305 for cutting and stirring, then the cut samples are equivalently added into three groups of culture test tubes 5 filled with agar culture medium, then a bottle stopper 501 is covered, and the three groups of culture test tubes are numbered from left to right, namely a No. 1 bottle, a No. 2 bottle and a No. 3 bottle;

adjusting the oxygen content in the three groups of culture test tubes 5 to ensure that the oxygen concentration in the No. 1 bottle, the No. 2 bottle and the No. 3 bottle is in a high oxygen, oxygen-free and proper range to form a control group for subsequent culture and detection operations, after 24-36 h culture, equivalently sampling and placing samples in the No. 1 bottle, the No. 2 bottle and the No. 3 bottle into the first liquid storage tank 702 and the second liquid storage tank 705 by using a sampling suction pipe 503, performing morphological observation on the sample liquid in the first liquid storage tank 702 by turning on a background lamp 701 by using a photoelectric microscope to judge whether salmonella exists, detecting the pH value of the sample liquid in the second liquid storage tank 705 by using an acid-base detector 1201, and judging whether salmonella exists in the sample liquid by combining optical observation of the photoelectric microscope if the pH value of the sample liquid in the second liquid storage tank 705 is acidic;

in order to reduce the interference of morphologically less-different-appearance pathogenic bacteria on the optical identification result of salmonella, the heating electric net 405 is started, then the bottle No. 1, the bottle No. 2 and the bottle No. 3 which are placed in the placing rack 4 are heated, the heating time is set for 4 minutes through the timer 402, then the steps are repeated, the steps are compared with the pH value after primary detection, if the pH value is not changed, the salmonella is judged to be contained, otherwise, other high-temperature resistant species of pathogenic bacteria are detected, and the strains are determined to be detected additionally;

after the detection is finished, the sample liquid in the first liquid storage tank 702 and the second liquid storage tank 705 on the surface of the sample observation frame 7 is poured, then the sample liquid is placed in the cleaning basin 102, the suction pump 10 is started, and the clean water in the cleaning water barrel 9 is pumped to the cleaning water faucet 101 for cleaning.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims (2)

1. The utility model provides an egg food safety in production uses biochemical check out test set with early warning suggestion, is including examining test table (1) and rack (4), its characterized in that: a placing frame (4) is installed at the top of the detection table (1), three groups of sample observation frames (7) which are arranged at equal intervals are placed at the top of the detection table (1), and the sample observation frames (7) are positioned in front of the placing frame (4);

the liquid level indicator is characterized by comprising a groove at the top of the sample observation frame (7), a transparent cover plate (703) is installed on the inner wall of the groove through a shaft rod, a first liquid storage groove (702) is formed in the bottom wall of the groove, a background lamp (701) is installed in the sample observation frame (7) in an embedded mode, the background lamp (701) is located below the first liquid storage groove (702), a second liquid storage groove (705) is formed in the top of the sample observation frame (7), the second liquid storage groove (705) is located on one side of the first liquid storage groove (702), an alarm lamp (704) is installed at the top of the sample observation frame (7), and the alarm lamp (704) is located in front of the second liquid storage groove (705);

a limiting hole (401) is formed in the top of the placing frame (4), and a culture test tube (5) is embedded in the limiting hole (401);

the bottom wall of the placing frame (4) is provided with a heating groove (404), the bottom wall of the heating groove (404) is provided with a heating electric mesh sheet (405), the top of the heating electric mesh sheet (405) is provided with an asbestos net (403), the top of the asbestos net (403) is contacted with the bottom of the culture test tube (5), the bottom wall of the placing frame (4) is provided with timers (402) which are arranged at equal intervals, the timers (402) and the heating groove (404) are arranged at intervals, and the timers (402) are electrically connected with the heating electric mesh sheet (405);

the top of the culture test tube (5) is provided with a bottle stopper (501) in a embedding manner, an air inlet pipe (504) is installed inside the bottle stopper (501) in a penetrating manner, an oxygen sensor (505) is installed on the inner wall of the air inlet pipe (504), a display screen (502) is installed on the surface of the air inlet pipe (504), the display screen (502) is electrically connected with the oxygen sensor (505), a sampling suction pipe (503) is installed inside the bottle stopper (501) in a penetrating manner, and the sampling suction pipe (503) is located on one side of the air inlet pipe (504);

a cladding pipe (8) is hermetically arranged in the air inlet pipe (504), an oxygen air feed pipe (801) and a nitrogen air feed pipe (802) are arranged in the cladding pipe (8), and the nitrogen gas feeding pipe (802) is positioned at one side of the oxygen gas feeding pipe (801), the tail end of the nitrogen gas feeding pipe (802) extends to the lower end of the interior of the culture test tube (5), an air cylinder (805) is arranged in the detection table (1), a partition plate is arranged in the air cylinder (805), the partition plate divides the interior of the air storage cylinder (805) into a nitrogen chamber (803) and an oxygen chamber (804), and the oxygen chamber (804) is positioned at one side of the nitrogen chamber (803), one end of the nitrogen gas feeding pipe (802) penetrates through the detection platform (1) and extends to the interior of the nitrogen chamber (803), one-degree eye of the oxygen gas supply pipe (801) penetrates through the detection table (1) and extends to the inside of the oxygen gas cavity (804);

electronic valves are arranged on the surfaces of the oxygen gas feeding pipe (801) and the nitrogen gas feeding pipe (802), one-way valves are arranged inside the oxygen gas feeding pipe (801) and the nitrogen gas feeding pipe (802), and the direction of the one-way valves inside the oxygen gas feeding pipe (801) in the middle group of the culture test tubes (5) is opposite to that of the nitrogen gas feeding pipe (802);

the sample detection device is characterized in that a cleaning bucket (9) is arranged inside the detection platform (1), the cleaning bucket (9) is located on one side of the air storage cylinder (805), a suction pump (10) is arranged on the front face of the cleaning bucket (9), the input end of the suction pump (10) is connected with the cleaning bucket (9) through a water pipe, a cleaning faucet (101) is arranged at the top of the detection platform (1), the cleaning faucet (101) is located in front of the side of the sample observation frame (7), a cleaning basin (102) is arranged at the top of the detection platform (1), the cleaning basin (102) is located in front of the cleaning faucet (101), and the output end of the suction pump (10) is connected with the cleaning faucet (101) through a water pipe;

a photoelectric microscope box (11) is arranged inside the detection table (1), the photoelectric microscope box (11) is positioned in front of the air storage cylinder (805), a slide rail (6) is arranged at the top of the detection table (1), the slide rail (6) is positioned between the placing frame (4) and the sample observation frame (7), and a movable door (2) is arranged on the front side of the detection table (1) through a hinge;

the back of the detection platform (1) is connected with an installation rack (3), a rotary table is installed at the top of the installation rack (3), an expansion rod (301) is installed at the top of the rotary table, an extension platform (302) is installed at the top of the expansion rod (301), a driving motor (303) is installed at the top of the extension platform (302), an output end of the driving motor (303) penetrates through the extension platform (302) and is provided with a stirring rod (304), a stirring drum (305) is installed at the top of the detection platform (1) and is located on one side of the placement rack (4), and cutting blades are installed on the surface of the stirring rod (304) in a surrounding mode;

a storage box (12) is installed at the top of the sample observation frame (7), an acid-base detector (1201) is placed in the storage box (12), and the acid-base detector (1201) is electrically connected with an alarm lamp (704).

2. The biochemical detection equipment with the early warning and prompting function for the egg food safety production as claimed in claim 1, wherein the detection equipment comprises the following working steps:

s1, before biochemical detection operation is carried out on related samples of egg food safety production by using the detection equipment, the samples are placed in a mixing drum (305), then a driving motor (303) is started, a mixing rod (304) is driven to cut and stir the samples in the mixing drum (305), then the cut samples are added into three groups of culture test tubes (5) filled with agar culture medium in equal amount, then a bottle stopper (501) is covered, and the three groups of culture test tubes are numbered from left to right and are respectively a No. 1 bottle, a No. 2 bottle and a No. 3 bottle;

s2, starting an electronic valve on the surface of an oxygen supply pipe (801) in the No. 1 bottle, injecting high-content oxygen into the bottle, closing the electronic valve on the surface of a nitrogen supply pipe (802), so that the inside of the No. 1 bottle contains oxygen with the concentration of more than 10%, simultaneously starting the electronic valves on the surfaces of the oxygen supply pipe (801) in the No. 2 bottle and the nitrogen supply pipe (802), conveying nitrogen into the inside of the No. 2 bottle through the nitrogen supply pipe (802), extruding and floating the oxygen in the No. 2 bottle, transferring the oxygen into an oxygen chamber (804) through the oxygen supply pipe (801), so that the inside of the No. 2 bottle is in an oxygen-free environment, similarly aiming at the No. 3 bottle, starting the electronic valve on the surface of the oxygen supply pipe (801), conveying oxygen with the oxygen concentration of 4% -8% into the inside of the No. 3 bottle, and further ensuring that the oxygen concentrations in the No. 1 bottle, the No. 2 bottle and the No. 3 bottle are in a high-oxygen state, Anaerobic and appropriate range, forming a control group for subsequent culture detection operation;

s3, after 24h-36h of culture, equally sampling the samples in the No. 1 bottle, the No. 2 bottle and the No. 3 bottle by using a sampling suction pipe (503) and putting the samples into the first liquid storage tank (702) and the second liquid storage tank (705), immediately turning over a transparent cover plate (703) to cover the top of the first liquid storage tank (702), taking out a photoelectric microscope in a photoelectric microscope box (11) to be embedded and installed on the surface of a slide rail (6), observing the sample liquid form in the first liquid storage tank (702) of an opening background lamp (701) by using the photoelectric microscope, judging whether salmonella exists or not by using the method, then detecting the pH value of the sample liquid in the second liquid storage tank (705) by using an acid-base detector (1201), and judging whether salmonella exists in the sample liquid by combining optical observation of a photoelectric microscope if the pH value of the sample liquid in the second liquid storage tank (705) is acidic;

s4, in order to reduce the interference of morphologically less-different-appearance pathogenic bacteria on the optical identification result of the salmonella, starting a heating electric net (405), then heating the bottle No. 1, the bottle No. 2 and the bottle No. 3 placed in a placing rack (4), setting the heating time for 4 minutes through a timer (402), repeating S3, comparing the heating time with the pH value after the first S3 detection, judging that the salmonella is contained if the pH value is unchanged, otherwise, detecting other high-temperature-resistant pathogenic bacteria, and determining the strains additionally;

s5, after the detection is finished, the photoelectric microscope is placed back into the photoelectric microscope box (11), then the sample liquid in the first liquid storage tank (702) and the second liquid storage tank (705) on the surface of the sample observation frame (7) is poured, then the sample liquid is placed into the cleaning basin (102), the suction pump (10) is started, and the clean water in the cleaning water bucket (9) is pumped to the cleaning water faucet (101) for cleaning.

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