CN114308147A - Detection chip, nucleic acid detection box and nucleic acid detection equipment - Google Patents
Detection chip, nucleic acid detection box and nucleic acid detection equipment Download PDFInfo
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- CN114308147A CN114308147A CN202110604893.0A CN202110604893A CN114308147A CN 114308147 A CN114308147 A CN 114308147A CN 202110604893 A CN202110604893 A CN 202110604893A CN 114308147 A CN114308147 A CN 114308147A
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Abstract
A detection chip comprises a first cover plate, a second cover plate, a conductive part and two first driving electrodes, wherein the conductive part comprises a first surface and a second surface which are arranged oppositely, the first surface and the second surface are respectively adjacent to the first cover plate and the second cover plate and enclose a channel, the channel comprises a detection path, the channel is used for bearing detection liquid, and the detection liquid is provided with charges; the two first driving electrodes are electrically connected with the conductive part; after the conductive part is electrified, a driving force is generated between the conductive part and the detection liquid, and the driving force is used for driving the detection liquid to move towards a direction away from the conductive part. The detection chip can prevent detection liquid from being accidentally separated from a detection path and being adsorbed on the side wall of the conductive part, and ensure that the amplification reaction is normally carried out. The present invention also provides a nucleic acid detecting cassette and a nucleic acid detecting apparatus.
Description
Technical Field
The invention relates to a detection chip, a nucleic acid detection box and nucleic acid detection equipment.
Background
At present, detection aiming at molecular diagnosis, morphology, immunology and the like is mostly carried out by adopting a microfluidic detection chip.
The structure of the traditional detection chip comprises an upper cover plate, a lower cover plate and a spacing layer positioned between the upper cover plate and the lower cover plate, wherein the upper cover plate, the spacing layer and the lower cover plate jointly form a channel for bearing detection liquid. In the detection process, the detection liquid drops move in different areas in the channel to finish the nucleic acid amplification process, but when operation problems or other factors cause the liquid drops to be adsorbed on the side wall of the spacing layer due to electrostatic action, the experiment fails because the liquid drops cannot move in a specified path.
Disclosure of Invention
In view of the above, in order to overcome at least one of the above drawbacks, it is necessary to provide a detection chip.
In addition, the application also provides a nucleic acid detecting box containing the detecting chip and a nucleic acid detecting device.
The invention provides a detection chip, which comprises: the first cover plate, the second cover plate, the conductive part and the two first driving electrodes. The conductive part comprises a first surface and a second surface which are oppositely arranged, the first surface and the second surface are respectively abutted to the first cover plate and the second cover plate, the first cover plate, the conductive part and the second cover plate are enclosed to form a channel, the channel comprises a detection path, the channel is used for bearing detection liquid, and the detection liquid is charged. The two first driving electrodes are electrically connected with the conductive part and used for electrifying or powering off the conductive part. After the conductive part is electrified, a driving force is generated between the conductive part and the detection liquid, and the driving force is used for driving the detection liquid to move towards a direction away from the conductive part so as to enable the detection liquid to move to the detection path.
In an embodiment of the present invention, the first driving electrode is disposed between the first surface and the first cover plate or the second cover plate, and the first driving electrode is electrically connected to the first surface.
In an embodiment of the application, the first cover plate is provided with two first grooves corresponding to the first surface, each of the first grooves is provided with one of the first driving electrodes, the first surfaces corresponding to the two first grooves protrude in a direction away from the second surface to form two first bumps, each of the first bumps is accommodated in a corresponding one of the first grooves, and the first bumps are electrically connected to the first driving electrodes.
In the embodiment of the application, the first cover plate corresponds the first surface is provided with a first groove, the second cover plate corresponds the second surface is provided with a second groove, and the first groove and the second groove are internally provided with one first driving electrode. The first surface protrudes towards the direction far away from the second surface to form a first bump, the first bump is contained in the first groove, and the first bump is electrically connected with one first driving electrode. The second surface protrudes towards the direction far away from the first surface to form a second bump, the second bump is contained in the second groove, and the second bump is electrically connected with the other first driving electrode.
In an embodiment of the application, the conductive portion is provided with two third grooves, and the two first driving electrodes are respectively disposed in the two third grooves and electrically connected to the conductive portion.
In an embodiment of the application, the conductive portion includes a conductive portion body and a first conductive layer disposed on a surface of the conductive portion body on a side close to the channel, and the first conductive layer is electrically connected to the two first driving electrodes.
In the embodiment of the application, the detection chip further comprises a driving component, the driving component comprises a driving loop arranged on the first cover plate close to the surface of one side of the second cover plate, a second conducting layer arranged on one side of the first cover plate close to the second cover plate, a first dielectric layer arranged on one side of the second cover plate close to the driving loop, and a second dielectric layer arranged on one side of the first cover plate close to the second conducting layer, wherein the channel is formed between the first dielectric layer and the second dielectric layer, and the driving loop forms the detection path.
In the embodiment of the application, the driving circuit comprises a plurality of second driving electrodes arranged in an array and all control electrodes electrically connected with the second driving electrodes, and the second driving electrodes are powered on or powered off with the second conducting layer to drive the detection liquid to move between two adjacent second driving electrodes.
The invention also provides a nucleic acid detection box, which comprises a box body, a detection chip and a connector. The detection chip is arranged in the box body and is the detection chip. The connector is electrically connected with the driving assembly and the first driving electrode respectively. Wherein, the detection chip is used for carrying out nucleic acid amplification reaction on the detection solution to obtain a nucleic acid amplification product.
The invention also provides nucleic acid detection equipment which comprises a host and a detection box installation groove. The detection box mounting groove is arranged on the host machine, the detection box mounting groove is used for detachably mounting the nucleic acid detection box, and the nucleic acid detection box is the nucleic acid detection box.
Compared with the prior art, the detection chip provided by the invention has the advantages that the structural design is simple, the assembly is convenient, and the detection liquid adsorbed on the side wall of the conductive part can return to the detection path through the design of the conductive part and the first driving electrode, so that the normal operation of nucleic acid amplification reaction is ensured.
Drawings
Fig. 1 is a schematic structural diagram of a detection chip according to an embodiment of the present invention.
Fig. 2 is a schematic cross-sectional structure diagram of a detection chip according to an embodiment of the present invention.
Fig. 3 is a schematic structural diagram of a driving circuit in a detection chip according to an embodiment of the present invention.
Fig. 4 is a schematic cross-sectional structure diagram of a detection chip according to a second embodiment of the present invention.
Fig. 5 is a schematic cross-sectional view of a detection chip according to a third embodiment of the invention.
Fig. 6 is a schematic cross-sectional view of a detection chip according to a fourth embodiment of the invention.
Fig. 7 is a schematic cross-sectional view of a detection chip according to a fifth embodiment of the invention.
Fig. 8 is a schematic cross-sectional view of a detection chip according to a sixth embodiment of the invention.
Fig. 9 is a schematic structural diagram of a detection chip according to an embodiment of the invention, in which a detection liquid is adsorbed on a conductive portion.
Fig. 10 is a schematic structural diagram of a detection chip according to an embodiment of the present invention, in which a detection liquid is driven off a conductive portion.
FIG. 11 is a schematic structural view of a nucleic acid detecting cassette according to an embodiment of the present invention.
FIG. 12 is a schematic structural view of a nucleic acid detecting apparatus according to an embodiment of the present invention.
Description of the main elements
The following detailed description will further illustrate the invention in conjunction with the above-described figures.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When a component is referred to as being "connected" to another component, it can be directly connected to the other component or intervening components may also be present. When a component is referred to as being "disposed on" another component, it can be directly on the other component or intervening components may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.
The system embodiments described below are merely illustrative, and the division of the modules or circuits is merely a logical division, and other divisions may be realized in practice. Furthermore, it is obvious that the word "comprising" does not exclude other elements or steps, and the singular does not exclude the plural. Several units or means recited in the system claims may also be implemented by one and the same unit or means in software or hardware. The terms first, second, etc. are used to denote names, but not any particular order.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1 to fig. 3, a detection chip 100 according to an embodiment of the invention includes a first cover plate 1, a second cover plate 2, a conductive portion 3, and two first driving electrodes 4. The conductive part 3 comprises a first surface 31 and a second surface 32 which are oppositely arranged, the first surface 31 and the second surface 32 are respectively arranged adjacent to the first cover plate 1 and the second cover plate 2, the first cover plate 1, the conductive part 3 and the second cover plate 2 are enclosed to form a channel 5, the channel 5 comprises a detection path 6, the channel 5 is used for bearing a detection liquid 7, and the detection liquid 7 is charged. The two first driving electrodes 4 are electrically connected to the conductive part 3, and are used for powering on or powering off the conductive part 3. After the conductive part 3 is electrified, a driving force is generated between the conductive part 3 and the detection liquid 7, and the driving force is used for driving the detection liquid 7 to move in a direction away from the conductive part 3, so that the detection liquid 7 moves to the detection path 6. The detection chip 100 is used for performing a nucleic acid amplification reaction, and a detection solution 7 containing a nucleic acid sample is introduced into the channel 5, and the detection solution 7 is present in the form of liquid beads in the channel 5.
Referring to fig. 2 and fig. 3, the detecting chip 100 further includes a driving component 9, the driving component 9 includes a driving circuit 91 disposed on a side of the first cover plate 1 close to the second cover plate 2, a first dielectric layer 92 disposed on a side of the driving circuit 91 close to the second cover plate 2, a second conductive layer 93 disposed on a side of the second cover plate 2 close to the first cover plate 1, and a second dielectric layer 94 disposed on a side of the second conductive layer 93 close to the first cover plate 1, wherein the channel 5 is formed between the first dielectric layer 92 and the second dielectric layer 94, and the driving circuit 91 forms the detecting path 6. The movement of the detection liquid 7 in the channel 5 according to the defined detection path 6 can be achieved by energizing or de-energizing the drive circuit 91 and the second electrically conductive layer 93.
In this embodiment, as shown in fig. 2 and fig. 3, the driving circuit 91 includes a plurality of second driving electrodes 911 arranged in an array and a control electrode 912 electrically connected to all the second driving electrodes 911. Specifically, the driving circuit 91 is a Thin Film Transistor (TFT) driving circuit, and since the detection liquid 7 has conductivity, the detection liquid 7 can move in the channel 5 along the predetermined detection path 6 by combining with the Dielectric wetting-On-Dielectric (EWOD) principle. By using the TFT principle, a circuit between one of the second driving electrodes 911 and the second conductive layer 93 can be selectively turned on or off, so that the voltage between the second driving electrode 911 and the second conductive layer 93 is changed to change the wetting characteristics between the detection liquid 7 and the first dielectric layer 92 and the second dielectric layer 92, thereby controlling the movement of the detection liquid 7 in the channel 5 according to the predetermined detection path 6. As shown in fig. 2, the detection liquid 7 moves on the electrode I, the electrode H and the electrode G, and when the detection liquid 7 is on the electrode H, a voltage is applied between the electrode G and the second conductive layer 93, a voltage Vd is applied to the electrode G, and the voltage between the electrode H and the second conductive layer 93 is disconnected, at which time the wetting characteristics between the detection liquid 7 and the first dielectric layer 92 and the second dielectric layer 94 are changed, so that the liquid-solid contact angle between the electrode H and the detection liquid 7 becomes larger, and the liquid-solid contact angle between the electrode G and the detection liquid 7 becomes smaller, thereby urging the detection liquid 7 to move from the electrode H to the electrode G.
In this embodiment, the first dielectric layer 92 and the second dielectric layer 94 are both insulating hydrophobic layers, and may be specifically teflon coatings, which can play a role of insulating hydrophobic, and on the other hand can make the detection liquid 7 move more smoothly in a specified path, thereby avoiding the liquid bead from breaking during the moving process.
In this embodiment, referring to fig. 2, the driving circuit 91 is disposed on the surface of the first cover plate 1 near the channel 5. The driving circuit 91 may be formed by a metal etching method or an electroplating method.
In this embodiment, referring to fig. 3, the control electrode 912 is integrated on the same edge of the first cover plate 1, so that the connection between the control electrode 912 and the connector can be facilitated.
Referring to FIG. 3, the driving circuit 91 can be divided into a plurality of regions, namely, a sample application region A, a reagent storage region B, a plurality of nucleic acid amplification regions C, and a liquid discharge region D, according to different applications. The second cover plate 2 is further provided with a sample adding slot 22 corresponding to the sample adding region A, the sample adding slot 22 is communicated with the sample adding region A, and the detection liquid 7 is added to the sample adding region A from the sample adding slot 22. The reagent storage region B is used to store a fluorescent reagent (e.g., a fluorescent dye or a fluorescent probe). The detection solution 7 performs a nucleic acid amplification reaction in the nucleic acid amplification region C, which may include a plurality of regions, and the number of the specific regions may be determined according to the actual detection requirement.
Referring to fig. 2 and fig. 3, the specific moving path of the detection liquid 7 in the detection chip 100 is: after entering the sample addition region A, the detection solution 7 moves to the nucleic acid amplification region C according to a predetermined path under the drive of the second drive electrode 911 to perform an amplification reaction; when the amplification reaction is completed, the amplified product moves to the reagent storage area B to be mixed with the fluorescent reagent, so that a nucleic acid amplification product combined with the fluorescent reagent is obtained; the uniformly mixed nucleic acid amplification product moves to the liquid outlet area D under the drive of the second drive electrode 911 for the next detection. In the process that the detection liquid 7 moves on the detection path 6, if the detection liquid 7 is adsorbed on the side wall of the conductive part 3 due to operation problems or electrostatic attraction, the negative charges of the conductive part 3 neutralize the positive charges of the detection liquid 7, so that the detection liquid 7 is completely negatively charged, a repulsive driving force is formed between the detection liquid 7 and the conductive part 3, and the detection liquid 7 is driven away from the side wall of the conductive part 3 under the action of the repulsive force and returns to the detection path 6 again, thereby ensuring the normal operation of the nucleic acid amplification reaction. The design of the conductive part 3 and the first driving electrode 4 skillfully utilizes the principle that like charges repel each other, and solves the problem that the detection solution can be accidentally separated from the detection path and cannot normally perform amplification reaction.
The first driving electrode 4 and the conductive part 3 can be electrically connected in various ways. Referring to fig. 2, in the present embodiment, the first driving electrode 4 is disposed between the first cover plate 1 and the first surface 31 of the conductive portion 3, and the first driving electrode 4 is in contact with and electrically connected to the conductive portion 3, and in order to ensure the sealing property of the channel 5, a sealing material may be filled between the first surface 31 and the surface of the first cover plate 1 close to the second cover plate 2. In the present embodiment, both the mounting of the first drive electrode 4 and the connection to the external power supply are relatively easy to implement.
In this embodiment, an opening 95 is formed in the first dielectric layer 92, the first driving electrode 4 is embedded in the opening 95, two opposite surfaces of the first driving electrode 4 are respectively in contact with the first surface 31 and the surface of the first cover plate 1 close to the second cover plate 2, and the first dielectric layer 92 fills a gap between the first surface 31 and the first cover plate 1, so as to seal the channel 5.
In this embodiment, in order to make the first driving electrode 4 sufficiently contact with the first surface 31 and ensure the stability of the electrical connection, a protrusion 33 may be disposed on the first surface 31, and the protrusion 33 is inserted into the opening 95 to make the first driving electrode 4 contact and electrically connect.
In the present embodiment, the first drive electrode 4 may be an electrode sheet.
Referring to fig. 4, in the second embodiment, two first grooves 11 are formed in a contact area of the first cover plate 1 and the first surface 31, one first driving electrode 4 is disposed in each first groove 11, two first bumps 34 are formed by protruding the first surface 31 of each first groove 11 in a direction away from the second surface 32, each first bump 34 is accommodated in a corresponding first groove 11, and the first bumps 34 are in contact with and electrically connected to the first driving electrode 4 located in the first groove 11. That is, two first grooves 11 for accommodating the first driving electrodes 4 are disposed on the first cover plate 1, and the first bumps 34 on the conductive part 3 are used to realize the contact and electrical connection between the conductive part 3 and the first driving electrodes 4. In this embodiment, the first driving electrode 4 is accommodated in the first recess 11 of the first cover plate 1, so that the sealing performance of the channel 5 is not affected, and the connection stability between the first driving electrode 4 and the conductive part 3 is improved.
In the present embodiment, the first drive electrode 4 is an electrode sheet.
Referring to fig. 5, in the third embodiment, a first groove 11 is formed in a contact region of the first cover plate 1 and the first surface 31, a second groove 21 is formed in a contact region of the second cover plate 2 and the second surface 32, and a first driving electrode 4 is disposed in each of the first groove 11 and the second groove 21. In addition, the first surface 31 protrudes in a direction away from the second surface 32 to form the first bump 34, the first bump 34 is accommodated in the first groove 11, and the first bump 34 is in contact with and electrically connected to a first driving electrode 4. The second surface 32 protrudes in a direction away from the first surface 31 to form a second bump 35, the second bump 35 is accommodated in the second groove 21, and the second bump 35 is in contact with and electrically connected to another first driving electrode 4. In the present embodiment, the first recess 11 and the second recess 21 are provided in the first cover plate 1 and the second cover plate 2, respectively, and the two first drive electrodes 4 are accommodated in the recesses, respectively, whereby the upper and lower surfaces of the conductive portion 3 can be connected to a power supply, and a voltage can be applied to the conductive portion 3. In this embodiment, the first driving electrode 4 is accommodated in the first recess 11 and the second recess 21, so that the sealing property of the channel 5 is not affected and the connection stability between the first driving electrode 4 and the conductive part 3 is improved.
In the present embodiment, the first drive electrode 4 is an electrode sheet.
Referring to fig. 6, in the fourth embodiment, the conductive portion 3 is provided with two third grooves 36, and two first driving electrodes 4 are respectively disposed in the two third grooves 36 and are in contact with and electrically connected to the conductive portion 3. In the present embodiment, two third grooves 36 are formed on the conductive portion 3 for accommodating the first driving electrode 4, so as to avoid forming grooves on the first cover plate 1 and the second cover plate 2, thereby facilitating the formation of the third grooves 36 and the assembly of the detection chip 100. In addition, the position of the third groove 36 disposed on the conductive portion 3 may be specifically designed according to actual requirements, and specifically, the opening of the third groove 36 may be disposed on the first surface 31 and/or the second surface 32, and may also be disposed on the side wall of the conductive portion 3 away from the channel 5.
In the present embodiment, the opening of the third groove 36 is disposed on the sidewall of the conductive portion 3 away from the channel 5, which is more convenient for the first driving electrode 4 to be led out to be electrically connected with the power supply, and the sealing performance of the channel 5 is not affected.
In the present embodiment, the first drive electrode 4 is an electrode sheet.
Referring to fig. 7, in a fifth embodiment, the conductive part 3 includes a conductive part body 37 and a first conductive layer 38 disposed on a surface of the conductive part body 37 near one side of the channel 5, and the first conductive layer 38 is electrically connected to two first driving electrodes 4. In the present embodiment, the conductive portion 3 does not need to be entirely conductive, and only the first conductive layer 38 needs to be coated or bonded on the side wall of the conductive portion main body 37 near the channel 5. In addition, at this time, the first driving electrode 4 may be electrically connected to the first conductive layer 38 in any form, as long as the first conductive layer 38 is electrically connected to the power supply, so as to ensure the sealing property of the channel 5.
Referring to fig. 8, in the sixth embodiment, the first driving electrode 8 may also be a long electrode with a certain aspect ratio, such as a needle-shaped or rod-shaped electrode, one end of the first driving electrode 8 is fixed on the conductive part 3, and the other end is connected to a power supply. By adopting the first driving electrode 8 of the embodiment, no groove needs to be formed, the forming difficulty is reduced, the cost is reduced, the assembly is convenient, and the circuit leading-out of the conductive part 3 is more convenient.
According to the invention, two first driving electrodes (4 and 8) are respectively connected with the positive electrode and the negative electrode of a power supply, and a conductive part 3 is powered on or powered off through the two first driving electrodes (4 and 8), specifically, as shown in fig. 2, the two first driving electrodes (4 and 8) are used for supplying negative voltage to the conductive part 3, so that the conductive part 3 is charged with negative charge after being powered on, and the conductive part 3 with negative charge adsorbs detection liquid 7 to the side wall of the conductive part 3 close to a channel 5 under the action of electrostatic force. As shown in fig. 9, the negative charges on the conductive part 3 neutralize the positive charges of the detection liquid 7, and the detection liquid 7 is charged only with negative charges. As shown in FIG. 10, in conjunction with FIG. 3, at this time, the same kind of charges repel each other, and the detection liquid 7 is pushed away from the side wall of the conductive part 3 by the repulsive force between the conductive part 3 and the detection liquid 7, and enters the detection path 6, whereby the nucleic acid amplification reaction proceeds. The conductive part 3 and the first driving electrode 4 are arranged, so that the problem that the detection liquid 7 is adsorbed on the side wall of the conductive part 3, cannot enter a detection path and cannot realize subsequent nucleic acid amplification reaction can be avoided.
Referring to FIG. 11, the present invention also provides a nucleic acid detecting cassette 200 including the detecting chip 100, wherein the nucleic acid detecting cassette 200 includes a cassette body 201 and a connector 202. The detecting chip 100 is disposed in the box 201, and the connector 202 is electrically connected to the first driving electrode 4 and the driving component 9 in the detecting chip 100 respectively.
Referring to FIG. 12, the present invention further provides a nucleic acid detecting apparatus 300, wherein the nucleic acid detecting apparatus 300 comprises a host 301 and the nucleic acid detecting cassette 200 as described above, the host 301 is provided with a cassette mounting groove 302, and the nucleic acid detecting cassette 200 is mounted in the cassette mounting groove 302.
Compared with the prior art, the detection chip provided by the invention has the advantages that the structural design is simple, the assembly is convenient, and the detection liquid adsorbed on the side wall of the conductive part can return to the detection path through the design of the conductive part and the first driving electrode, so that the normal operation of nucleic acid amplification reaction is ensured.
Claims (10)
1. A detection chip, comprising:
a first cover plate;
a second cover plate;
the conductive part comprises a first surface and a second surface which are oppositely arranged, the first surface and the second surface are respectively adjacent to the first cover plate and the second cover plate, the first cover plate, the conductive part and the second cover plate are enclosed to form a channel, the channel comprises a detection path, the channel is used for bearing detection liquid, and the detection liquid is charged; and
the two first driving electrodes are electrically connected with the conductive part and used for electrifying or powering off the conductive part;
after the conductive part is electrified, a driving force is generated between the conductive part and the detection liquid, and the driving force is used for driving the detection liquid to move towards a direction away from the conductive part so as to enable the detection liquid to move to the detection path.
2. The detecting chip of claim 1, wherein the first driving electrode is disposed between the first surface and the first cover plate or the second cover plate, and the first driving electrode is electrically connected to the first surface.
3. The detecting chip of claim 1, wherein the first cover plate has two first recesses corresponding to the first surfaces, each of the first recesses has one of the first driving electrodes therein, the first surfaces corresponding to the two first recesses are protruded in a direction away from the second surface to form two first bumps, each of the first bumps is received in a corresponding one of the first recesses, and the first bumps are electrically connected to the first driving electrodes.
4. The detecting chip of claim 1, wherein the first cover plate has a first recess corresponding to the first surface, the second cover plate has a second recess corresponding to the second surface, and the first recess and the second recess have a first driving electrode therein;
the first surface protrudes towards the direction far away from the second surface to form a first bump, the first bump is contained in the first groove, and the first bump is electrically connected with one first driving electrode;
the second surface protrudes towards the direction far away from the first surface to form a second bump, the second bump is contained in the second groove, and the second bump is electrically connected with the other first driving electrode.
5. The detecting chip of claim 1, wherein the conductive portion has two third recesses, and two of the first driving electrodes are respectively disposed in the two third recesses and electrically connected to the conductive portion.
6. The detecting chip according to claim 1, wherein the conductive portion includes a conductive portion body and a first conductive layer disposed on a surface of the conductive portion body on a side thereof adjacent to the channel, the first conductive layer being electrically connected to the two first driving electrodes.
7. The detection chip of claim 1, further comprising a driving component, wherein the driving component comprises a driving loop disposed on a surface of one side of the first cover plate close to the second cover plate, a second conductive layer disposed on one side of the second cover plate close to the first cover plate, a first dielectric layer disposed on one side of the driving loop close to the second cover plate, and a second dielectric layer disposed on one side of the second conductive layer close to the first cover plate, the first dielectric layer and the second dielectric layer forming the channel therebetween, and the driving loop forms the detection path.
8. The detection chip of claim 7, wherein the driving circuit comprises a plurality of second driving electrodes arranged in an array and a control electrode electrically connected to all of the second driving electrodes, and the second driving electrodes and the second conductive layer are electrically connected or disconnected for driving the detection solution to move between two adjacent second driving electrodes.
9. A nucleic acid detecting cassette characterized by comprising:
a box body;
the detection chip is arranged in the box body, and the detection chip is the detection chip according to any one of claims 1 to 8; and
the connector is electrically connected with the driving assembly and the first driving electrode respectively;
wherein, the detection chip is used for carrying out nucleic acid amplification reaction on the detection solution to obtain a nucleic acid amplification product.
10. A nucleic acid detecting apparatus characterized by comprising:
a host;
a cartridge mounting groove provided in the main body, the cartridge mounting groove being for detachably mounting a nucleic acid detecting cartridge according to claim 9.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17/477,982 US20220097052A1 (en) | 2020-09-30 | 2021-09-17 | Detection chip, nucleic acid detection kit, and nucleic acid detection device |
| EP21199486.8A EP3978122A1 (en) | 2020-09-30 | 2021-09-28 | Detection chip, nucleic acid detection kit, and nucleic acid detection device |
| PCT/CN2021/122431 WO2022068937A1 (en) | 2020-09-30 | 2021-09-30 | Nucleic acid testing device |
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063085368P | 2020-09-30 | 2020-09-30 | |
| US202063085385P | 2020-09-30 | 2020-09-30 | |
| US63/085368 | 2020-09-30 | ||
| US63/085385 | 2020-09-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114308147A true CN114308147A (en) | 2022-04-12 |
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Family Applications (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110604893.0A Pending CN114308147A (en) | 2020-09-30 | 2021-05-31 | Detection chip, nucleic acid detection box and nucleic acid detection equipment |
| CN202110602285.6A Pending CN114317220A (en) | 2020-09-30 | 2021-05-31 | Nucleic acid detecting cassette and nucleic acid detecting apparatus |
| CN202110604898.3A Pending CN114317250A (en) | 2020-09-30 | 2021-05-31 | Heating structure, detection chip, nucleic acid detection box and nucleic acid detection equipment |
| CN202110735245.9A Pending CN114317238A (en) | 2020-09-30 | 2021-06-30 | Nucleic acid detecting cassette and nucleic acid detecting apparatus |
| CN202110735243.XA Pending CN114317224A (en) | 2020-09-30 | 2021-06-30 | Nucleic acid detection host and nucleic acid detection equipment |
Family Applications After (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110602285.6A Pending CN114317220A (en) | 2020-09-30 | 2021-05-31 | Nucleic acid detecting cassette and nucleic acid detecting apparatus |
| CN202110604898.3A Pending CN114317250A (en) | 2020-09-30 | 2021-05-31 | Heating structure, detection chip, nucleic acid detection box and nucleic acid detection equipment |
| CN202110735245.9A Pending CN114317238A (en) | 2020-09-30 | 2021-06-30 | Nucleic acid detecting cassette and nucleic acid detecting apparatus |
| CN202110735243.XA Pending CN114317224A (en) | 2020-09-30 | 2021-06-30 | Nucleic acid detection host and nucleic acid detection equipment |
Country Status (3)
| Country | Link |
|---|---|
| JP (3) | JP2022058149A (en) |
| CN (5) | CN114308147A (en) |
| TW (4) | TW202214866A (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20220099621A1 (en) * | 2020-09-30 | 2022-03-31 | Icare Diagnostics International Co. Ltd. | Nucleic acid detection kit and nucleic acid detection device |
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| JP2022058149A (en) | 2022-04-11 |
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