CN114276927A - Folding carrier column for bioreactor - Google Patents

Abstract

The invention discloses a folding carrier column for a bioreactor, which comprises a folding carrier column unit, wherein the folding carrier column unit comprises a first cylinder body and a second cylinder body which are sleeved with each other, a first window is arranged on the side wall of the first cylinder body and is used for enabling a culture solution filled from the lower end of an opening in the first cylinder body to flow in a single direction under the action of pressure and to be filled into a first cavity which is sealed between the first cylinder body and the second cylinder body, a second window is arranged on the side wall of the second cylinder body and is used for enabling the culture solution filled into the first cavity to further flow in the single direction under the action of pressure and to be filled into a second cavity between the second cylinder body and the inner wall of the bioreactor, the second cavity is filled into the first cylinder body again through the lower end of the first cylinder body to form circulation, and a folding carrier with a plurality of folding surfaces is arranged in the first cavity. The invention can take out the carrier from the reactor, and can prevent cell death caused by extracting cell culture solution, thereby obviously improving the surface area of the carrier and realizing large-scale culture of various animal cells.

Description

Folding carrier column for bioreactor

Technical Field

The invention relates to the technical field of biological pharmaceutical equipment, in particular to a folding carrier column for a bioreactor.

Background

The form of cell growth carriers is various, but roughly divided into two major categories, i.e., GE microcarriers and sheet carriers.

At present, both microcarriers and sheet carriers are distributed on the bottom of a bioreactor in an irregular manner when put into use. The capacity of the bioreactor is difficult to be enlarged due to the influence of cell metabolism and the distribution of cell culture solution.

In particular, in the fluidized bed reactor, when a cell culture medium is extracted, the cell culture medium is also extracted, which makes the operation difficult.

Meanwhile, after the cell culture is finished, the granular cell culture carrier is difficult to repeatedly treat, and the use efficiency of the bioreactor is influenced.

Disclosure of Invention

The invention aims to overcome the defects of the prior art and provides a folding carrier column for a bioreactor.

One technical solution of the present invention to achieve the above object is:

a folding carrier column for a bioreactor, comprising:

the foldable carrier column unit comprises a first cylinder and a second cylinder which are mutually sleeved and longitudinally suspended in the reactor, the upper end of the first cylinder is closed, a first window is arranged on the side wall of the first cylinder and used for enabling a culture solution filled from the lower end of an opening in the first cylinder to flow in a single direction under the action of pressure and to be filled into a first cavity sealed between the first cylinder and the second cylinder through the first window, a second window is arranged on the side wall of the second cylinder and used for enabling the culture solution filled into the first cavity to flow in a single direction under the action of pressure and to be filled into a second cavity between the second cylinder and the inner wall of the reactor through the second window and enabling the culture solution filled into the first cavity to flow in a second direction under the action of pressure and to be filled into the first cylinder again through the lower end of the first cylinder by the second cavity to form circulation, the first cavity is provided with a folding carrier with a plurality of folding surfaces.

Further, the number of the folding carrier column units is one to multiple, and when the number of the folding carrier column units is multiple, the folding carrier column units are suspended in the reactor in a surrounding manner.

Further, a plurality of the carrier column units are suspended in the reactor such that the upper and lower ends of the first tubular bodies of the respective carrier column units are sequentially abutted against each other, wherein the first tubular bodies of the respective carrier column units are communicated with each other, the upper end of the first tubular body of the uppermost one of the carrier column units is closed, and the lower end of the first tubular body of the lowermost one of the carrier column units is open so as to fill the culture medium into the respective first tubular bodies.

Further, the plurality of folding carrier column units are suspended in the reactor in such a manner that the upper ends and the lower ends of the respective first column bodies are in butt joint in sequence to form a folding carrier column unit group, in the folding carrier column unit group, the first column bodies of the folding carrier column units are communicated with each other, the upper end of the first column body of the uppermost one of the folding carrier column units is closed, the lower end of the first column body of the lowermost one of the folding carrier column units is open and is used for filling a culture solution into the respective first column bodies, the plurality of folding carrier column unit groups are suspended in the reactor in a surrounding manner.

Further, the carrier comprises a folding carrier which takes the first cylinder body as the center and is distributed in a radial shape towards the second cylinder body and is provided with a plurality of folding surfaces.

Further, the carrier comprises a first carrier and a second carrier which are stacked and arranged in the first cavity in an up-and-down mode; wherein the number of folds of the radially folded surfaces on the first carrier is greater than the number of folds of the radially folded surfaces on the second carrier, and/or the height of the first carrier is less than the height of the second carrier.

Further, the carrier material comprises a nonwoven.

Furthermore, a grid and a mesh are arranged on the side wall of the second cylinder in a surrounding mode, and the mesh serves as the second window.

Further, the folding carrier column unit is configured to rotate and revolve in the reactor, the culture liquid is pumped up into the first cylinder from the open lower end of the first cylinder, flows unidirectionally through the first window and is filled into the first cavity under the combined action of pressure and centrifugal force, and further flows unidirectionally through the second window and is filled into the second cavity under the combined action of pressure and centrifugal force.

Furthermore, the upper end of the first cavity is sealed by an end cover, the carrier is fixed in the first cavity by a filter screen arranged between the upper end of the carrier and the end cover, and the upper end and the lower end of the first cylinder are respectively provided with a rotary interface.

Compared with the prior art, the invention has the following advantages:

(1) the traditional small granular carrier is replaced by the integral flaky folding carrier, the traditional technical mode is broken through, the labor cost is greatly reduced, and the loss of the carrier is reduced.

(2) The foldable carrier is arranged in the foldable carrier column unit and can be taken out along with the whole carrier column, the defect that the traditional manual net bag is fished is overcome, and the problem that the cell culture carrier is extracted together to cause cell death when cell culture solution is extracted in the prior art is perfectly solved.

(3) By designing the folding carrier with a plurality of folding surfaces which are distributed in a radial shape, not only a good supporting surface for cell growth is provided, but also the surface area of the cell culture carrier is obviously improved, and various animal cells can be effectively cultured in a large scale.

(4) Through scientific design of the carrier column structure, a one-way circulation passage of the culture solution is successfully established between the interior (the first cavity) of the carrier column and the interior (the second cavity and the third cavity) of the reactor, and the circulation effect of the culture solution is improved.

(5) By arranging a plurality of carrier column units and different combination forms among the carrier column units, the capacity of the bioreactor can be enlarged, and the productivity is obviously improved.

(6) The carriers are arranged in layers in the carrier column unit in an up-and-down stacking mode, so that the structural strength of the carriers in the carrier column is improved, and the problem of small bearing capacity of the carriers is successfully solved.

(7) Through set up the culture solution flow window on the carrier column unit side, can utilize rotation and the revolution of carrier column in the reactor, make the culture solution can carry out the circulation of flowing under the combined action of pressure and centrifugal force, obviously improved the proportion of dissolved oxygen, effectively promoted cell growth.

Drawings

Fig. 1-3 are schematic structural views of a folding carrier column for a bioreactor according to a preferred embodiment of the present invention.

Fig. 4 is a schematic diagram of a transverse arrangement of a carrier in a foldable carrier column unit according to a preferred embodiment of the present invention.

Fig. 5 is a schematic diagram of a longitudinal arrangement structure of a carrier in a foldable carrier column unit according to a preferred embodiment of the present invention.

Fig. 6 is a schematic view of an assembly structure of a foldable carrier column unit according to a preferred embodiment of the invention.

FIG. 7 is a schematic diagram of a folding carrier column for a bioreactor according to a preferred embodiment of the present invention applied to a bioreactor.

Detailed Description

In order that the technical solution of the invention may be better understood, the invention will now be described in detail by means of specific embodiments.

Please refer to fig. 1. The folding carrier column for the bioreactor comprises one to a plurality of folding carrier column units 14. Wherein the folding carrier column unit 14 includes a first cylinder 141 and a second cylinder 142; the first and second cylinders 141 and 142 are nested in parallel with each other, and can be suspended in the reactor in a longitudinal direction.

In a preferred embodiment, the first cylinder 141 and the second cylinder 142 comprise cylindrical cylinders, and the cylindrical cylinders of the first cylinder 141 and the second cylinder 142 are coaxially engaged with each other.

Please refer to fig. 1. When in use, the upper end of the first cylinder 141 is a closed end, and the lower end of the first cylinder 141 is an open end. Further, a seal is formed between the upper end of the first cylinder 141 and the upper end of the second cylinder 142, and a seal is also formed between the lower end of the first cylinder 141 and the lower end of the second cylinder 142, so that a first closed cavity 146 (refer to fig. 4) is formed between the side wall of the first cylinder 141 and the side wall of the second cylinder 142.

The interior of first cavity 146 is used to position carrier 143 which provides a cell growth support surface. The invention adopts the folding carrier 143 with a plurality of folding surfaces arranged in the first cavity 146, so as to provide the carrier 143 with larger surface area for cell culture, thereby improving the cell culture productivity and efficiency.

Please refer to fig. 2. A first window 1411 is disposed on the sidewall of the first cylinder 141, and a second window 1421 is disposed on the sidewall of the second cylinder 142. The first window 1411 and the second window 1421 communicate through the first cavity 146, so that the carrier 143 disposed in the first cavity 146 communicates with the inside of the first cylinder 141 through the first window 1411, while communicating with the outside of the column unit 14 of the folding carrier 143 through the second window 1421.

Thus, the culture liquid 13 can be filled into the first cylinder 141 from the open lower end of the first cylinder 141 by the action of the pressure applied to the culture liquid 13. Since the upper end of the first cylinder 141 is in a closed state, the culture solution 13 entering the inside of the first cylinder 141 flows into the first cavity 146 in one direction through the first window 1411 under pressure, and fills the first cavity 146, so that the carriers 143 in the first cavity 146 are immersed in the culture solution 13, and cells attached to the surfaces of the carriers 143 are cultured.

Meanwhile, because the sidewall of the second cylinder 142 is further provided with the second window 1421, the culture solution 13 in the first cavity 146 will continue to flow through the second window 1421 through the carrier 143 under pressure, and further flow into the second cavity 11 between the second cylinder 142 and the inner wall of the reactor in one direction, i.e. flow into the chamber of the reactor 10 between the folding carrier column unit 14 and the inner wall of the reactor 10, and can flow back from the second cavity 11, and under pressure, the culture solution is refilled into the first cylinder 141 through the lower end of the first cylinder 141, so as to form a dynamic circulation of the culture solution 13, as shown in fig. 7.

In a preferred embodiment, the first window 1411 and the second window 1421 may be bar-shaped windows arranged in a horizontal direction, that is, the horizontal length of the first window 1411 and the second window 1421 is greater than the vertical height of the first window 1411 and the second window 1421.

Also, the first window 1411 and the second window 1421 may be respectively provided in plural numbers on the side walls of the first cylinder 141 and the second cylinder 142, so that the folded surfaces of the carrier 143 can be exposed through each of the first window 1411 and the second window 1421.

In a preferred embodiment, in the vertical direction, any two adjacent layers of the first windows 1411 and any two adjacent layers of the second windows 1421 may be arranged in a staggered manner, for example, a distribution pattern between the delta-shaped first windows 1411 and a distribution pattern between the delta-shaped second windows 1421 may be formed, as shown in fig. 2.

Further, in the horizontal direction, each (each layer of) first windows 1411 may be located at the same vertical height as one (one layer of) second windows 1421 of the opposite side. Alternatively, the first windows 1411 and the second windows 1421 on the opposite side may be staggered in the vertical direction. Still alternatively, the number of the first windows 1411 disposed on the first cylinder 141 and the number of the second windows 1421 disposed on the second cylinder 142 may be the same or different.

In a preferred embodiment, the sidewalls of the first window 1411 and the second window 1421 have an inclination angle towards the outside, respectively, so that the first window 1411 and the second window 1421 become wide-angle windows opened towards the outside.

Please refer to fig. 3. In a preferred embodiment, the side wall of the second cylinder 142 is uniformly provided with a grid and a mesh around the side wall of the second cylinder 142, wherein the mesh can be used as the second window 1421, which can maximally promote the circulation of the culture solution 13. The folding carrier column unit 14 can be supported by the first cylinder 141 to keep the stability of the whole structure.

The first window 1411 and the second window 1421 function as an inlet and an outlet, respectively, for the flow renewal of the culture liquid 13 disposed on the closed first cavity 146.

Please refer to fig. 4. In a preferred embodiment, the carrier 143 is a foldable carrier 143 which is radially arranged from the axis of the first cylinder 141 to the side wall of the second cylinder 142 when viewed from the vertical direction, the foldable carrier 143 has a plurality of folded surfaces formed by repeated folding, and each folded surface is connected end to end and surrounds the first cylinder 141 to form a vertical cylindrical structure having a radial folded surface profile, which has a relatively large surface area capable of providing a cell culture support surface.

Please refer to fig. 5. In a preferred embodiment, carrier 143 may include, viewed in a horizontal orientation, a first carrier 1431 and a second carrier 1432; also, a first carrier 1431 and a second carrier 1432 are disposed in the first cavity 146 in a stacked manner one on top of the other.

In a preferred embodiment, the number of folds of the radial folds in the upper first carrier 1431 may be configured to be greater than the number of folds of the radial folds in the lower second carrier 1432.

In a preferred embodiment, the vertical height of first carrier 1431 may be set to be less than the vertical height of second carrier 1432.

Thus, the second carriers 1432 having a relatively small number of folds and a relatively high height can be used as a support structure for the first carriers 1431 having a relatively large number of folds and a relatively low height, which can eliminate the problem of structural collapse caused by the increase in weight after the carriers 143 are soft and fully loaded with growing cells, and the convection capability of the culture solution 13 in the first cavity 146 can be promoted by the structural independence between the first carriers 1431 and the second carriers 1432.

In a preferred embodiment, the carrier 143 material may comprise a non-woven fabric. But the invention is not limited thereto.

Please refer to fig. 6-7. In a preferred embodiment, when the folding carrier column units 14 are plural, the folding carrier column units 14 may be suspended in the reactor 10 (the second cavity 11) in a surrounding manner. For example, the six foldable carrier column units 14 are uniformly suspended in the reactor 10 in such a manner as to surround the center (guide cylinder 12) of the reactor 10, so that the volume of the reactor 10 can be enlarged by correspondingly enlarging the area of the reactor 10, the yield of cell culture can be significantly increased, and mass-efficient culture of various animal cells can be realized.

In a preferred embodiment, when there are a plurality of the folding carrier column units 14, each folding carrier column unit 14 may be suspended in the reactor 10 in such a manner that the upper and lower ends of the first cylinder 141 are in butt joint with each other. The first cylinders 141 of the respective foldable carrier column units 14 are connected to each other by abutting the upper ends and the lower ends thereof, the upper end of the first cylinder 141 of the uppermost foldable carrier column unit 14 is closed, and the lower end of the first cylinder 141 of the lowermost foldable carrier column unit 14 is opened to fill the culture solution 13 into the upper first cylinders 141. Accordingly, the volume of the reactor 10 can be enlarged by correspondingly increasing the height of the reactor 10, and the yield of cell culture can be remarkably increased, thereby realizing large-scale and effective culture of various animal cells.

In a preferred embodiment, a plurality of folding carrier column units 14 may be suspended in the reactor 10 in such a manner that the upper and lower ends of the respective first cylinder 141 are in butt joint with each other in sequence to form a folding carrier column unit group. In each folding carrier column unit group, the first cylinders 141 of the folding carrier column units 14 are communicated with each other, the upper end of the first cylinder 141 of the uppermost folding carrier column unit 14 is closed, and the lower end of the first cylinder 141 of the lowermost folding carrier column unit 14 is opened for filling the culture solution 13 into the upper first cylinders 141. And, a plurality of folding carrier column unit groups are provided so that the folding carrier column unit groups are suspended in the reactor 10 in a surrounding manner. Thus, the volume of the reactor 10 can be further increased by increasing the area and height of the reactor 10 at the same time, and a larger scale of cell culture can be realized.

Please refer to fig. 7. In a preferred embodiment, the folded carrier column units 14 may be configured to rotate within the reactor 10 and revolve around the center of the reactor 10. This arrangement has an advantage that when the culture liquid 13 is pumped up from the open lower end of the first cylinder 141 into the first cylinder 141 by the agitation pump 16, it can smoothly flow in one direction through the first window 1411 and be filled into the first cavity 146 under the combined action of the pumping pressure and the rotational centrifugal force, and further smoothly flow in one direction through the second window 1421 and be filled into the second cavity 11 under the combined action of the pressure and the centrifugal force. Meanwhile, the rotation and revolution arrangement of the folding carrier column unit 14 can agitate the culture solution 13 in the reactor 10 (second cavity 11) to a certain extent, so as to further increase the Dissolved Oxygen (DO) content, thereby improving the cell culture efficiency.

Please refer to fig. 1. In a preferred embodiment, the lower end of the first cavity 146 is closed by a bottom plate 145.

In one example, the lower end of the first cylinder 141 may be exposed to the lower end surface of the second cylinder 142, and the bottom plate 145 may have a mounting hole corresponding to the outer diameter of the first cylinder 141. The bottom plate 145 is sleeved on the first cylinder 141 from the lower end of the first cylinder 141 through a mounting hole, and the inner edge and the outer edge of the bottom plate 145 are respectively fixed with the lower end side wall of the first cylinder 141 and the lower end face of the second cylinder 142, so as to seal the lower end of the first cavity 146.

In a preferred embodiment, an external rotation port may be provided on the lower end surface of the first barrel 141 exposed out of the bottom plate 145 for rotatably connecting with the bottom surface of the second cavity 11 of the reactor 10, or rotatably connecting with an internal rotation port at the upper end of the first barrel 141 of another foldable carrier column unit 14 to be stacked therebelow. Thus, when the cultivation is completed, the carrier column unit 14 of the folding type can be easily lifted out of the reactor 10 by rotating the carrier column unit 14 of the folding type to detach the carrier column unit 14 of the folding type from the bottom surface of the second cavity 11 of the reactor 10.

The plurality of contiguous carrier column units 14 may also be conveniently separated.

In a preferred embodiment, the upper end of the first cavity 146 is closed by a removable end cap 144.

In one example, the upper end of the first cylinder 141 may be disposed flush with or close to the upper end of the second cylinder 142, and the end cap 144 may be disposed in a flange shape such that the flange of the end cap 144 corresponds to the upper end of the first cylinder 141. An inner rotary interface is arranged on the inner wall of the flange opening of the end cover 144, and is used for matching with a sealing cover to seal the upper end of the first cylinder 141 in a rotary manner, or is communicated with an outer rotary interface at the lower end of the first cylinder 141 of another folding carrier column unit 14 which needs to be stacked above in a rotary manner.

Further, the outer circumference of the end cover 144 is provided with an external thread, and the inner wall of the upper end of the second cylinder 142 is correspondingly provided with an internal thread. After the carrier 143 is placed in the first cavity 146, when the first cavity 146 is sealed, the flange opening of the end cover 144 is aligned with the upper end of the first cylinder 141, the internal rotation port on the inner wall of the flange opening of the end cover 144 is matched with the external thread on the outer side of the upper end of the first cylinder 141, meanwhile, the external thread on the periphery of the end cover 144 is matched with the internal thread on the inner wall of the upper end of the second cylinder 142, the end cover 144 is simultaneously screwed on the upper ends of the first cylinder 141 and the second cylinder 142, the first cavity 146 is sealed, and the structure of the folding carrier column unit 14 is stable.

In an alternative embodiment, the end cap 144 and the flap may be integral.

In a preferred embodiment, a filter screen may also be disposed in the first cavity 146 below the end cap 144, and the carrier 143 may be secured in the first cavity 146 by the filter screen disposed between the upper end thereof and the end cap 144. The screen may serve to prevent cells on the carrier 143 from adsorbing on the inner surface of the end cap 144.

Please refer to fig. 7. A folding carrier column for a bioreactor of the present invention can be applied to a bioreactor such as that of FIG. 7. The interior of the reactor 10 is partitioned into a second cavity 11 located above and a third cavity 15 located below the second cavity 11.

The guide shell 12 is arranged in the second cavity 11. The upper end of the guide shell 12 is an opening, and the lower end of the guide shell 12 is communicated with a third cavity 15.

An agitator pump 16 is provided in the third cavity 15 below the open lower end of the draft tube 12. The blades 161 of the mixer pump 16 may be disposed toward the open lower end of the guide shell 12.

The upper end of the reactor 10 can be provided with a cavity cover 111; the chamber cover 111 may be provided with a plurality of introduction valves 112. The aeration tube 121 provided in the draft tube 12 can be aerated by the introduction valve 112, and the culture solution 13 can be added to the reactor 10. A support bracket 17 may also be provided on the lower end of the reactor 10. A lead-out valve communicating with the second chamber 15 may be further provided on the lower end of the reactor 10 for replacing the culture liquid 13 and the like.

The second cavity 11 and the third cavity 15 can be separated by a partition 18, and the partition 18 can be horizontally and fixedly arranged on the inner wall of the reactor 10. Wherein, the partition plate 18 is provided with a first diversion port and a second diversion port; the lower end of the guide cylinder 12 can be communicated with the third cavity 15 through a first guide opening on the partition plate 18, and the lower end of the first cylinder 141 on each folding carrier column unit 14 can be communicated with the third cavity 15 through being screwed with a corresponding second guide opening on the partition plate 18.

By turning on the stirring pump 16, the culture solution 13 flowing downward from the second cavity 11 into the third cavity 15 through the open upper end of the guide cylinder 12 is stirred and pressurized, and the culture solution 13 is pressure-fed from the open lower end of the first cylinder 141 into the first cylinder 141. Since the upper end of the first cylinder 141 is in a closed state, the culture solution 13 entering the inside of the first cylinder 141 flows into the first cavity 146 in one direction through the first window 1411 under pressure, and fills the first cavity 146, so that the carriers 143 in the first cavity 146 are immersed in the culture solution 13, and cells attached to the surfaces of the carriers 143 are cultured.

Meanwhile, because the sidewall of the second cylinder 142 is further provided with the second window 1421, the culture solution 13 in the first cavity 146 continues to flow through the second window 1421 through the carrier 143 under the pressure, and is further filled into the second cavity 11 in a unidirectional flow manner. By setting the liquid level of the culture solution 13 in the second cavity 11 to be higher than the open upper end of the guide shell 12, the culture solution 13 can flow back downwards into the third cavity 15 from the open upper end of the guide shell 12 through overflow of the culture solution 13, and the culture solution 13 is pressure-fed and filled into the first cylinder 141 again through the lower end of the first cylinder 141 under the pressure applied by the stirring pump 16, so that dynamic circulation of the culture solution 13 is formed. Wherein, under the stable dynamic circulation state, the liquid level of the culture solution 13 in the second cavity 11 is higher than the upper end of the opening of the guide shell 12, so that the guide shell 12, the third cavity 15 and the folding carrier column unit 14 are all filled with the culture solution 13.

It should be understood by those skilled in the art that the above embodiments are only for illustrating the present invention and are not to be used as a limitation of the present invention, and that the changes and modifications of the above embodiments are within the scope of the claims of the present invention as long as they are within the spirit and scope of the present invention.

Claims (10)

1. A folding carrier column for a bioreactor, comprising:

the foldable carrier column unit comprises a first cylinder and a second cylinder which are mutually sleeved and longitudinally suspended in the reactor, the upper end of the first cylinder is closed, a first window is arranged on the side wall of the first cylinder and used for enabling a culture solution filled from the lower end of an opening in the first cylinder to flow in a single direction under the action of pressure and to be filled into a first cavity sealed between the first cylinder and the second cylinder through the first window, a second window is arranged on the side wall of the second cylinder and used for enabling the culture solution filled into the first cavity to flow in a single direction under the action of pressure and to be filled into a second cavity between the second cylinder and the inner wall of the reactor through the second window and enabling the culture solution filled into the first cavity to flow in a second direction under the action of pressure and to be filled into the first cylinder again through the lower end of the first cylinder by the second cavity to form circulation, the first cavity is provided with a folding carrier with a plurality of folding surfaces.

2. The folding carrier column for bioreactors according to claim 1, wherein said folding carrier column unit is one to a plurality, and when said folding carrier column unit is a plurality, each of said folding carrier column units is suspended in a surrounding manner in said bioreactor.

3. The folding carrier column for bioreactors according to claim 1, wherein the folding carrier column units are provided in plurality, and are suspended in the bioreactor so that the upper and lower ends of the first cylinders of the folding carrier column units are in sequential contact with each other, wherein the first cylinders of the folding carrier column units communicate with each other, the upper end of the first cylinder of the uppermost folding carrier column unit is closed, and the lower end of the first cylinder of the lowermost folding carrier column unit is open, and is used for filling culture medium into the first cylinders.

4. The folding carrier column according to claim 1, wherein a plurality of the folding carrier column units are suspended in the reactor so that the upper and lower ends of the respective first tubular bodies are in sequential contact with each other to form a folding carrier column unit group, the first tubular bodies of the folding carrier column units are in communication with each other in the folding carrier column unit group, the upper end of the first tubular body of the uppermost one of the folding carrier column units is closed, the lower end of the first tubular body of the lowermost one of the folding carrier column units is open, and the first tubular bodies are filled with a culture medium therein, and the folding carrier column unit group is provided in plurality, and the folding carrier column unit groups are suspended in the reactor so as to surround each other.

5. The folding carrier column for bioreactors of claim 1, wherein said carrier comprises a folding carrier having a plurality of folding surfaces centered on said first cylinder and radiating toward said second cylinder.

6. The folding carrier column for a bioreactor of claim 5, wherein the carrier comprises a first carrier and a second carrier disposed in the first cavity in a top-to-bottom stack; wherein the number of folds of the radially folded surfaces on the first carrier is greater than the number of folds of the radially folded surfaces on the second carrier, and/or the height of the first carrier is less than the height of the second carrier.

7. The folded carrier column for a bioreactor according to claim 1, 5 or 6, wherein the carrier material comprises a non-woven fabric.

8. The folding carrier column for bioreactors of claim 1, wherein said second cylinder has a side wall with a mesh and a mesh, said mesh serving as said second window.

9. The folding carrier column for a bioreactor according to claim 1, wherein the folding carrier column unit is configured to rotate and revolve in the reactor, the culture solution is pumped up into the first cylinder from the open lower end of the first cylinder, flows unidirectionally through the first window and fills into the first cavity under a combined action of pressure and centrifugal force, and further flows unidirectionally through the second window and fills into the second cavity under a combined action of pressure and centrifugal force.

10. The folding carrier column for bioreactors of claim 1, wherein said first cavity is closed at its upper end by an end cap, said carrier is secured in said first cavity by a screen disposed between its upper end and said end cap, and said first cylinder is provided with rotary joints at its upper and lower ends.

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