CN114276389A - Method and system for purifying xylose by valve array type continuous chromatography - Google Patents
Method and system for purifying xylose by valve array type continuous chromatography Download PDFInfo
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- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 title claims abstract description 209
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 title claims abstract description 105
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 title claims abstract description 105
- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000004587 chromatography analysis Methods 0.000 title claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 27
- 239000011259 mixed solution Substances 0.000 claims abstract description 24
- 239000003480 eluent Substances 0.000 claims abstract description 21
- 239000000243 solution Substances 0.000 claims abstract description 15
- 239000002253 acid Substances 0.000 claims description 15
- 238000000926 separation method Methods 0.000 claims description 13
- 239000011347 resin Substances 0.000 claims description 12
- 229920005989 resin Polymers 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 8
- 238000001704 evaporation Methods 0.000 claims description 7
- 239000002245 particle Substances 0.000 claims description 7
- 150000001768 cations Chemical class 0.000 claims description 6
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 4
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 230000003301 hydrolyzing effect Effects 0.000 claims description 4
- 239000004571 lime Substances 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 239000008367 deionised water Substances 0.000 claims description 3
- 229910021641 deionized water Inorganic materials 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims 1
- 238000010828 elution Methods 0.000 abstract description 5
- 239000012535 impurity Substances 0.000 abstract description 4
- 230000005540 biological transmission Effects 0.000 abstract description 2
- 238000000746 purification Methods 0.000 abstract description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 8
- 238000013375 chromatographic separation Methods 0.000 description 8
- 238000000605 extraction Methods 0.000 description 6
- 150000003839 salts Chemical class 0.000 description 6
- 230000007062 hydrolysis Effects 0.000 description 5
- 238000006460 hydrolysis reaction Methods 0.000 description 5
- 150000002500 ions Chemical class 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 230000008020 evaporation Effects 0.000 description 4
- 238000005342 ion exchange Methods 0.000 description 4
- 239000000049 pigment Substances 0.000 description 4
- 239000003513 alkali Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 241000209149 Zea Species 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 238000002425 crystallisation Methods 0.000 description 2
- 230000008025 crystallization Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000000413 hydrolysate Substances 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 229920000297 Rayon Polymers 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 235000011114 ammonium hydroxide Nutrition 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 238000004042 decolorization Methods 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000909 electrodialysis Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L sulfate group Chemical group S(=O)(=O)([O-])[O-] QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 125000000969 xylosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)CO1)* 0.000 description 1
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Abstract
The invention provides a method and a system for purifying xylose by valve array type continuous chromatography, wherein the purification method comprises the following steps: the method comprises the steps of continuously adding a xylose mixed solution into a first set-position chromatographic column of a plurality of chromatographic columns connected in series, continuously extracting raffinate from a second set-position chromatographic column, continuously adding eluent into a third set-position chromatographic column, continuously extracting an extracting solution from the third set-position chromatographic column, allowing the rest of liquid to pass through the rest of chromatographic columns, adding the rest of liquid into the first set-position chromatographic column together with the xylose mixed solution, sequentially switching the chromatographic columns at set intervals according to the flow direction of the xylose mixed solution, wherein the distance between the third set-position chromatographic column and the first set-position chromatographic column is larger than the distance between the second set-position chromatographic column and the first set-position chromatographic column, and the switching direction is opposite to the flow direction of the xylose mixed solution. The method can well separate xylose from impurities, reduce the conductivity of the xylose solution by about 95 percent and improve the light transmission by more than 50 percent. The invention has continuous feeding, elution and collection and is easy to operate.
Description
Technical Field
The invention relates to a xylose processing technology, in particular to a method and a system for purifying xylose by valve array type continuous chromatography.
Background
Xylose is a pentose sugar with the molecular formula C5H10O5The crystal is fine needle-shaped, the appearance is white and fine crystal or powder, the taste is sweet, and the sweetness is equivalent to 0.7 of that of cane sugar; the melting point is 153-154 ℃. At present, the xylose is mainly prepared by using corncobs and viscose fiber squeezed alkali liquor as raw materials at home.
The production process of xylose mainly adopts a chemical method, and comprises the steps of hydrolyzing pentosan by adding acid to obtain xylose mixed liquor, and then carrying out processes such as decolorization, ion exchange or electrodialysis, evaporation concentration, crystallization separation and the like to obtain a xylose finished product. Because a certain amount of acid is required to be added as a catalyst for xylose hydrolysis, the pH value of xylose mixed liquor obtained after hydrolysis is low, the conductivity is high, and the color is dark.
In the traditional process, the hydrolysis liquid is desalted and pigment is decolored by a large amount of activated carbon, then acid and salt are removed by an ion exchange process for many times, chemical acid and chemical alkali are needed during ion exchange regeneration, the consumption is high, a large amount of waste water is generated, and the pollution is serious.
Disclosure of Invention
In order to reduce the pollution problem in the xylose processing, the invention adopts a valve array type continuous chromatographic separation system to decolor and desalinate the xylose mixed solution, thereby reducing the discharge of a large amount of sewage.
The technical scheme of the invention is as follows: a method for purifying xylose by valve array type continuous chromatography comprises the steps of continuously adding a xylose mixed solution into a first set-position chromatographic column of a plurality of chromatographic columns connected in series, continuously extracting raffinate from a second set-position chromatographic column, continuously adding eluent into a third set-position chromatographic column, continuously extracting extract from the third set-position chromatographic column, allowing the rest of liquid to pass through the rest of chromatographic columns, adding the rest of liquid and the xylose mixed solution into the first set-position chromatographic column, sequentially switching the chromatographic columns at set intervals according to the flowing direction of the xylose mixed solution, wherein the distance between the third set-position chromatographic column and the first set-position chromatographic column is larger than that between the second set-position chromatographic column and the first set-position chromatographic column, and the switching direction is opposite to the flowing direction of the xylose mixed solution. And the rest of liquid passing through the third set chromatographic column passes through the rest chromatographic columns, is mixed with the xylose mixed solution and is added into the first set chromatographic column to form circulation. The chromatographic column is switched by opening and closing a valve. The flowing speed of the xylose and impurities in the xylose mixed solution in the chromatographic resin along with the liquid carrier is different, so that the extracting solution and the raffinate can be obtained at the outlet of a specific chromatographic column. The extract is xylose solution, and the raffinate is rich in salt and pigment.
The chromatographic column adopts macroporous strong acid cation separation resin.
The particle size of the resin is 0.20-0.35 mm.
The xylose mixed liquor is prepared by adding acid into corncobs for hydrolysis, adjusting the pH value with lime, filtering to obtain xylose filtrate, and evaporating and concentrating the xylose filtrate to obtain the xylose mixed liquor.
The eluent is water, preferably deionized water, more preferably evaporative or steam condensate.
The feed-liquid ratio is 1: 1.2-2. The feed-liquor ratio refers to the ratio of the added xylose mixed liquor to the eluent.
The flow rate of the eluent is 1m/h-3 m/h.
Hydrolyzing the corncobs with sulfuric acid.
The conductivity of the xylose mixture is preferably not more than 30000. mu./cm, more preferably not more than 20000. mu./cm, still more preferably not more than 10000. mu./cm.
The content of dry substances in the extracting solution is 1-40 wt%. The dry matter is predominantly xylose.
The content of dry solids (dry matter) in the xylose mixed liquor is 1-70 wt%. The dry solids (dry matter) include xylose and some impurities.
The extract is subjected to evaporation and/or membrane concentration to obtain xylose.
The raffinate is subjected to evaporation and/or membrane concentration to obtain xylose byproduct.
The temperature of the xylose mixed solution is 0-100 ℃, preferably 20-80 ℃.
The temperature of the eluent is 0-100 ℃, preferably 20-80 ℃.
The invention provides a system for purifying xylose by valve array type continuous chromatography, which comprises a plurality of chromatographic columns which are arranged in series end to end and are provided with
A first set-position chromatographic column, wherein the xylose mixed solution continuously enters from the first set-position chromatographic column;
a second set-position chromatographic column, wherein raffinate is continuously extracted from an outlet of the second set-position chromatographic column;
the eluent continuously enters the third set-position chromatographic column, and the extracting solution is continuously extracted from the outlet of the third set-position chromatographic column;
the third set-point column is positioned downstream of the second set-point column in the direction of flow of the xylose mixture.
The chromatographic columns are switched sequentially at set intervals, and the switching direction is opposite to the flowing direction of the xylose mixed solution. The chromatographic column is switched to open and close by a valve.
The number of the chromatographic columns is below 36, preferably 30, 24, 20, 16, 12 or 8 chromatographic columns.
The chromatographic column adopts macroporous strong acid cation separation resin.
The particle size of the resin is 0.20-0.35 mm. The particle size of the resin has a critical influence on the separation, the particle size is large, the separation and uniform distribution of the feed liquid in the column can be not facilitated, the separation purity is not high, the particle size is small, the density of the stationary phase of the chromatographic separation column can be increased, the feed liquid cannot penetrate through the column easily, the separation speed and the separation effect are influenced, and the column pressure can be increased to cause the resin to be broken.
The system for separating xylose by valve array type continuous chromatography automatically controls continuous feeding, continuous elution and continuous extraction through a PLC.
Since the salts contained in the xylose solution are mainly generated by the processes of hydrolysis, acid addition and pH value adjustment, and mainly consist of cations and anions, the ions can be singly-charged ions or multiply-charged ions. The invention relates to the different retention times or elution volumes of xylose on a chromatographic column compared to salts, pigments. Xylose is mainly enriched in the extraction liquid phase, and salt and pigment are enriched in the raffinate phase.
The method and the system adopt the valve array type continuous chromatographic separation method and system to separate and purify the xylose mixed solution, the eluent can be recycled, the chromatographic column can be regenerated through the eluent at the same time, other regeneration solutions are not required to be added, the cost can be reduced, the use of acid and alkali is reduced, and the method is energy-saving and environment-friendly.
Valve array continuous chromatographic separation methods and systems are an industrial process for separating two compounds or ions in a liquid phase in a continuous manner, which takes advantage of different molecular properties (e.g., ion repulsion, hydrogen bonding, or molecular size) to provide a wide range of applications. Compared with the existing purification and desalination methods (such as distillation, crystallization or membrane separation, ion exchange), the method does not need an organic solvent and does not need acid-base or ammonia water solution, thereby obviously reducing the level of chemical additives and energy consumption. The chromatographic method can well separate xylose from impurities, reduce the conductivity of the xylose solution by about 95 percent and improve the light transmission by more than 50 percent. In particular, since the continuous chromatography system of the present invention is a continuous process of continuous feeding, elution and collection, it is easy to operate.
Drawings
FIG. 1 is a schematic flow diagram of a system for continuous chromatographic extraction of xylose in a valve array of example 1.
FIG. 2 is a schematic diagram of the structure of the system for continuous chromatographic extraction of xylose in a valve array of example 1.
Detailed Description
The present invention will be described in detail with reference to examples.
A method for separating and purifying xylose by valve array type continuous chromatography includes adding mixed xylose liquid to the first set-position chromatographic column of multiple series-connected chromatographic columns, extracting raffinate from the second set-position chromatographic column in flowing direction of mixed xylose liquid, adding eluent into the third set-position chromatographic column in flowing direction of mixed xylose liquid and extracting extract from the third set-position chromatographic column, adding the rest liquid into the first set-position chromatographic column together with mixed xylose liquid after the rest liquid passes through the rest chromatographic columns to form circulation, sequentially switching the chromatographic columns at set intervals according to flowing direction of mixed xylose liquid, and switching direction being opposite to flowing direction of mixed xylose liquid.
The xylose mixed liquor is prepared by hydrolyzing corn cob with acid, adjusting pH value with lime, filtering to obtain xylose filtrate, and evaporating and concentrating the xylose filtrate to obtain xylose mixed liquor. The content of dry solids in the xylose mixed liquor is 1-70 wt%. The corn cob is hydrolyzed with acid, which can be sulfuric acid, hydrochloric acid, nitric acid, phosphoric acid, etc., preferably sulfuric acid. If sulfuric acid is used as the hydrolysate in the xylose mixture, the main salt in the xylose mixture is sulfate. The conductivity of the xylose mixed solution is very high and is usually in the range of 30000-10000 mu/cm.
Water is used as eluent, preferably deionized water, more preferably evaporated or steam condensed water. The flow rate of the eluent is 1m/h-3 m/h.
The ratio of the material to the liquid is 1: 1.2-2. The feed-liquor ratio refers to the ratio of the added xylose mixed liquor to the eluent.
The method for separating and purifying xylose adopts a valve array type continuous chromatographic xylose separation system, comprises a plurality of chromatographic columns which are connected in series and are connected end to end, and the chromatographic columns are provided with
A first set-position chromatographic column, wherein the xylose mixed solution enters from the first set-position chromatographic column;
a second set-position chromatographic column, wherein raffinate is extracted from an outlet of the second set-position chromatographic column;
a third setting position chromatographic column, wherein eluent enters from the third setting position chromatographic column, and extracting solution is extracted from an outlet of the third setting position chromatographic column;
in the flowing direction of the xylose mixed solution, the second set-position chromatographic column is arranged at the downstream of the first set-position chromatographic column, and the third set-position chromatographic column is arranged at the downstream of the second set-position chromatographic column;
the chromatographic columns are switched sequentially at set intervals, and the switching direction is opposite to the flowing direction of the xylose mixed solution.
The number of the chromatographic columns is below 36, preferably 30, 24, 20, 16, 12 or 8 chromatographic columns.
The chromatographic column adopts macroporous strong acid cation separation resin.
The particle size of the resin is 0.20-0.35 mm.
The system for separating xylose by valve array type continuous chromatography automatically controls continuous feeding, continuous elution and continuous extraction through a PLC.
Example 1
As shown in the figure 1-2, the system for continuous chromatographic separation of xylose in a valve array comprises 8 chromatographic columns Z1-Z8 which are arranged in series end to end, wherein the chromatographic column Z5 is a first set-position chromatographic column, the chromatographic column Z7 is a second set-position chromatographic column, and the chromatographic column Z1 is a third set-position chromatographic column.
The xylose mixed liquid (material) enters from a chromatographic column Z5, flows through chromatographic columns Z6 and Z7 in sequence, raffinate is extracted at the outlet of the chromatographic column Z7, the rest of liquid continuously flows through the chromatographic columns Z8 and Z9, as the chromatographic column Z9 is connected with the chromatographic column Z1 in series, eluent-water is added into the chromatographic column Z1, extract is adopted at the outlet of the chromatographic column Z1, and the rest of liquid continuously flows through the chromatographic columns Z2, Z3 and Z4 and then enters into the chromatographic column Z5 together with the xylose mixed liquid to form circulation. In this process, the xylose feed mixture (feed) valve of column Z5 was opened, the raffinate valve of column Z7 was opened, the eluent (feed) valve and the extract valve of column Z1 were opened, and the remaining valves were closed.
At set intervals, the chromatographic columns are switched sequentially by opening and closing valves, and the switching direction is opposite to the flowing direction of the xylose mixed solution. Specifically, at set time intervals, such as 15 minutes or 18 minutes or 20 minutes, the xylose feed mixture (feed) valve of the column Z5 is closed, the raffinate valve of the column Z7 is closed, the eluent (feed) valve and the extract valve of the column Z1 are closed, the xylose feed mixture (feed) valve of the column Z4 is opened, the raffinate valve of the column Z6 is opened, and the eluent (feed) valve and the extract valve of the column Z8 are opened, and each time one column is switched in the direction opposite to the flowing direction of the xylose mixture, the distances between the first set-position column, the second set-position column, and the third set-position column are kept unchanged.
As shown in FIG. 2, the upper opening of each chromatographic column is provided with a feed valve and a water inlet valve, and the lower outlet is provided with a raffinate valve and an extract valve.
Example 2
The system of example 2 is similar to the structure of example 1, but the number of chromatography columns may be 30, 24, 20, 16 or 12 columns.
According to the number of chromatographic columns and the material separation condition, setting a first set-position chromatographic column, a second set-position chromatographic column and a third set-position chromatographic column, namely determining the distance among the first set-position chromatographic column, the second set-position chromatographic column and the third set-position chromatographic column, and carrying out corresponding feeding and discharging.
And then the chromatographic columns are switched according to the set interval time.
Example 3
The system for separating xylose by adopting valve array type continuous chromatography in the embodiment 1 is used for separating and purifying xylose mixed liquor, and comprises the following specific steps: adjusting the pH value of the corncob hydrolysate with lime, and performing flocculation filtration to obtain xylose filtrate, wherein various parameter indexes of the filtrate after evaporation concentration are that the dry matter content is 50-60 wt%, and the xylose accounts for 57-67% of the dry matter content; the xylose content is more than or equal to 90 percent after chromatographic separation; the temperature is 50-60 ℃, the conductivity is 10000-30000us/cm, the pH value is 5.0-6.0, the light transmittance is 10-30%, the xylose mixed solution enters from the upper part of a chromatographic column Z5, then raffinate is extracted from a chromatographic column Z7, eluent-water enters from the upper part of a chromatographic column Z1, and extract is extracted from an outlet of the chromatographic column Z1. Continuously feeding, eluting and extracting by a circulating pump, wherein the indexes of the extracting solution and the raffinate are shown as the following table:
the xylose solution passes through macroporous strong acid cation chromatographic separation resin in a valve array type chromatographic separation device, and the operating parameters in the system are as follows: the material-liquid ratio is 1:1.2-2, extracting solution: residual liquid extraction ratio 5: 4, switching time 15 min.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A method for purifying xylose by valve array type continuous chromatography is characterized in that xylose mixed liquor is continuously added into a first set-position chromatographic column of a plurality of chromatographic columns connected in series, raffinate is continuously extracted from a second set-position chromatographic column, eluent is continuously added into a third set-position chromatographic column, extract is continuously extracted from the third set-position chromatographic column, other liquid passes through the other chromatographic columns and then is added into the first set-position chromatographic column together with the xylose mixed liquor, according to the flowing direction of the xylose mixed liquor, the distance between the third set-position chromatographic column and the first set-position chromatographic column is larger than that between the second set-position chromatographic column and the first set-position chromatographic column, the chromatographic columns are sequentially switched at set intervals, and the switching direction is opposite to the flowing direction of the xylose mixed liquor.
2. The method of claim 1, wherein the xylose mixture is obtained by hydrolyzing corn cobs with acid, adjusting the pH with lime, filtering to obtain a xylose filtrate, and evaporating and concentrating the xylose filtrate to obtain the xylose mixture.
3. The method according to claim 1, wherein the eluent is water, preferably deionized water, more preferably evaporated or steam condensate.
4. The method of claim 1, wherein the eluent flow rate is between 1m/h and 3 m/h.
5. The method according to claim 1, wherein the feed-to-liquid ratio is 1: 1.2-2.
6. The method of claim 1, wherein the dry solids content of the xylose mixture is 1-70 wt.%.
7. A system for purifying xylose by valve array type continuous chromatography comprises a plurality of chromatographic columns which are arranged in series end to end and are provided with
A first set-position chromatographic column, wherein the xylose mixed solution enters from the first set-position chromatographic column;
a second set-position chromatographic column, wherein raffinate is extracted from an outlet of the second set-position chromatographic column;
a third setting position chromatographic column, wherein eluent enters from the third setting position chromatographic column, and extracting solution is extracted from an outlet of the third setting position chromatographic column;
in the flowing direction of the xylose mixed solution, a second set-position chromatographic column is arranged at the downstream of the first set-position chromatographic column, and a third set-position chromatographic column is arranged at the downstream of the second set-position chromatographic column;
the chromatographic columns are switched sequentially at set intervals, and the switching direction is opposite to the flowing direction of the xylose mixed solution.
8. The system according to claim 7, wherein the number of chromatography columns is below 36 columns, preferably 30, 24, 20, 16, 12 or 8 columns.
9. The system of claim 7, wherein the chromatographic column employs a macroporous strong acid cation separation resin.
10. The method of claim 9, wherein the resin has a particle size of 0.20 to 0.35 mm.
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