CN114209888A - Preparation method and application of injectable hydrogel for skin repair - Google Patents
Preparation method and application of injectable hydrogel for skin repair Download PDFInfo
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- CN114209888A CN114209888A CN202111601521.9A CN202111601521A CN114209888A CN 114209888 A CN114209888 A CN 114209888A CN 202111601521 A CN202111601521 A CN 202111601521A CN 114209888 A CN114209888 A CN 114209888A
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Images
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- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/52—Hydrogels or hydrocolloids
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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- A—HUMAN NECESSITIES
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
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- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3687—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by the use of chemical agents in the treatment, e.g. specific enzymes, detergents, capping agents, crosslinkers, anticalcification agents
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- A61L27/3683—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
- A61L27/3691—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment characterised by physical conditions of the treatment, e.g. applying a compressive force to the composition, pressure cycles, ultrasonic/sonication or microwave treatment, lyophilisation
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Abstract
The invention belongs to the technical field of skin repair gel, and particularly relates to a preparation method and application of injectable hydrogel for skin repair. The method comprises the steps of taking animal fat, carrying out tissue disruption treatment, repeatedly freezing and thawing, then respectively placing the animal fat in a sodium chloride solution, water and an emulsifier for ultrasonic oscillation treatment, rinsing the fat tissue, placing the rinsed fat tissue in an octylphenol polyoxyethylene ether solution for extraction, centrifuging, cleaning and freeze-drying to obtain fat tissue epimatrix powder, then preparing a hydrogel product, effectively destroying cell structures in the fat, releasing biological factors to attach to the fat tissue, simultaneously effectively removing grease substances in the fat, retaining the fat epimatrix structure, presenting a fiber structure, combining with a gel material to form a hydrogel product, retaining the biological activity of the fat tissue, and simultaneously enabling the product form of the hydrogel to be more suitable for skin repair.
Description
Technical Field
The invention belongs to the technical field of skin repair gel, and particularly relates to a preparation method and application of injectable hydrogel for skin repair.
Background
Fat transplantation began to enter the human vision as early as the 20 th century, and the development of fat transplantation has gone from large fat blocks to granular fat and then to nano-fat, and has undergone a series of development processes. Adipose tissue contains a large number of active structures, such as adipose stem cells, exosomes, adipose extracellular matrix, and the like. Among them, adipose-derived stem cells are only suitable for patients, have a small amount of exosomes, and have certain application limitations. The fat extracellular matrix has great advantages in the autologous or allogenic filling process, and compared with the traditional filler such as hyaluronic acid, the fat extracellular matrix has the following advantages, such as serving as a tissue scaffold and starting tissue repair on a defect part; the fat cell extracellular matrix can release growth factors and promote the growth of autologous tissues, and besides, the fat cell extracellular matrix removes main immunogenic structures, can be used by allogeneic or xenogeneic sources, is suitable for large-scale application, and increases economic benefits. But the conventional method for treating the adipose extracellular matrix limits its application range. For example, the fat extracellular matrix is prepared into matrigel by an enzyme digestion method, which changes the internal structure of the fat extracellular matrix to a certain extent and possibly reduces the effect of the fat extracellular matrix, or the fat extracellular matrix is prepared into powder and injected and filled after being mixed uniformly, and the method well retains the tissue activity, but cannot achieve good application effect due to loose tissues.
Disclosure of Invention
In view of the above problems, the present invention is directed to a method for preparing an injectable hydrogel for skin repair, which retains the structure of the fat extracellular matrix and maintains biological activity, and a hydrogel product obtained thereby is suitable for skin repair.
The technical content of the invention is as follows:
the invention provides a preparation method of injectable hydrogel for skin repair, which comprises the following steps:
1) preparing an adipose tissue extracellular matrix: taking animal fat, carrying out centrifugal dehydration, carrying out tissue disruption treatment on the fat, carrying out centrifugation again to remove water and grease, carrying out repeated freeze thawing on the treated fat, then respectively placing the fat in a sodium chloride solution, water and an emulsifier to carry out ultrasonic oscillation treatment, rinsing the fat tissue, then placing the rinsed fat tissue in an octylphenol polyoxyethylene ether solution to extract, centrifuging, cleaning and freeze-drying to obtain fat tissue epimatrix powder;
2) preparing alginate or chitosan into a solution with the concentration of 0.4-1 mg/mL, magnetically stirring for 3-5 hours, adding the adipose tissue extracellular matrix powder prepared in the step 1) to form adipose tissue extracellular matrix glue with the concentration of 2-10 mg/mL, uniformly stirring, and standing at 0-5 ℃ to obtain a tissue mixed solution;
3) adding calcium chloride solution into the tissue mixed solution, and uniformly stirring to obtain the hydrogel.
The specific operation of preparing the adipose tissue extracellular matrix in the step 1) is as follows: taking fat of a human body or a pig, centrifugally dewatering, performing tissue crushing treatment on the fat by using a low-temperature tissue grinder under the conditions of 65-80 Hz, 30-40 s and 3-5 cycles, centrifugally removing water and grease again, placing the treated fat in liquid nitrogen for 1.5-2 h, rewarming at 35-40 ℃, circulating for 3-4 times, then placing the fat in 1M sodium chloride solution, ultrasonically oscillating for 2h at 37 ℃, rinsing with distilled water, placing the fat in distilled water for ultrasonically oscillating for 1h, rinsing with distilled water, placing the fat in 4-5% of an emulsifier, ultrasonically oscillating for 1h at room temperature, rinsing with distilled water, then placing the fat in an octylphenol polyoxyethylene ether solution for 36h, replacing extract liquor every 12h to remove redundant lipid, repeatedly rinsing with distilled water for 3-5 times, centrifuging for 3 min at 2000g at 4 ℃, repeatedly rinsing the precipitate with distilled water, finally, freeze-drying the tissues by using a vacuum freeze dryer (the temperature is-65 ℃ and the freeze-drying time is 48 hours), preparing adipose tissue epimatrix powder by using a low-temperature grinding method, and directly freezing the powder at-80 ℃ for storage;
the frequency of the ultrasonic oscillation is 60-80 kHz, and the ultrasonic oscillation is processed for 1-3 hours;
the preparation of the emulsifier is as follows: in a vacuum environment, mixing fatty acid methyl ester and water, heating to 120-150 ℃, adding ethylene oxide, uniformly stirring, adding a sodium citrate and gamma-alumina composite catalyst, reacting for 1.5-2 h, cooling to 80-100 ℃, adding a gamma-alumina catalyst, continuing to react for 1.5-2 h, and cooling to normal temperature to obtain a fatty acid methyl ester ethoxylate product, which is beneficial to removing grease in adipose tissues, and meanwhile, the stability of the adipose tissues is kept, so that the tissues are prevented from loosening;
the mixing mass ratio of the fatty acid methyl ester and water in the step 1) is (6-8): 1;
the addition amount of the ethylene oxide is 3-5 times of the mass of the mixture of the fatty acid methyl ester and the water;
the addition amount of the sodium citrate and gamma-alumina composite catalyst is 2-3 wt%, wherein the composite mass ratio of the sodium citrate to the gamma-alumina is (3-5): (4-7);
the addition amount of the gamma-alumina catalyst is 1-2 wt%.
The octyl phenol polyoxyethylene ether solution in the step 1) is prepared by mixing octyl phenol polyoxyethylene ether with 8-10 g/L PBS solution, placing the mixture in a water bath at 35-40 ℃ and stirring for 1-2 hours, wherein the mixture can be fused with oil components in fat, so that phospholipid in the fat can be taken away more easily, and meanwhile, the fibrous structure of the fat is maintained;
the polymerization degree of the octyl phenol polyoxyethylene ether is 4 or 6;
the addition amount of the octyl phenol polyoxyethylene ether is 5-7 wt% of the PBS solution.
The alginate in the step 2) comprises sodium alginate, has good water absorption, is non-toxic to human bodies and can be absorbed by the human bodies;
and 3) the concentration of the calcium chloride is 2-3%.
The invention also provides the injectable hydrogel for skin repair prepared by the preparation method.
The invention also provides application of the injectable hydrogel in facial filling and wound treatment.
The invention has the following beneficial effects:
the prepared injectable hydrogel effectively destroys the cell structure in fat by crushing the adipose tissue and adopting the treatment of sodium chloride, emulsifier and extraction solution, releases biological factors to be attached to the adipose tissue, simultaneously effectively removes grease substances in the fat, retains the outer matrix structure of the fat, presents a fiber structure, is combined with a gel material to form a hydrogel product, retains the biological activity of the adipose tissue, simultaneously enables the product form of the hydrogel to be more suitable for skin repair, coats the hydrogel on the wounded skin, can promote tissue repair, can also be used for coating of the skin grafting, promotes the survival of the skin grafting, reduces the pain caused by friction generated by frequent drug change, can improve the photoaging degree of the skin when used on the skin, and thus maintains the healthy state of the skin.
Drawings
FIG. 1 is an electron micrograph of adipose tissue;
FIG. 2 is an electron micrograph of adipose tissue sonicated with sodium chloride, water and emulsifier;
FIG. 3 is an electron micrograph of the adipose tissue extracellular matrix after solution extraction;
FIG. 4 is a graph showing the change in the thickness of the epidermis of the skin of a rat under ultraviolet irradiation in the test example;
fig. 5 shows the change in dermal thickness of the skin of a rat under ultraviolet irradiation in the test example.
Detailed Description
The present invention is described in further detail in the following description of specific embodiments and the accompanying drawings, it is to be understood that these embodiments are merely illustrative of the present invention and are not intended to limit the scope of the invention, which is defined by the appended claims, and modifications thereof by those skilled in the art after reading this disclosure that are equivalent to the above described embodiments.
All the raw materials and reagents of the invention are conventional market raw materials and reagents unless otherwise specified.
Example 1
Preparation of an injectable hydrogel for skin repair:
1) preparing an adipose tissue extracellular matrix: taking fat of human body or pig, centrifuging to dewater, crushing the fat by using a low-temperature tissue grinder under the conditions of 70Hz, 35s and 4 cycles, centrifuging again to remove water and grease, and displaying that the cell structure is damaged but other impurities of the grease exist as shown in figure 1;
placing the treated fat in liquid nitrogen for 2h, rewarming at 37 ℃, circulating for 3 times, then placing in 1M sodium chloride solution, ultrasonically oscillating at 70 kHz for 2h at 37 ℃, rinsing with distilled water, then placing in distilled water for ultrasonically oscillating at 70 kHz for 1h, rinsing with distilled water, placing in 4% emulsifier, ultrasonically oscillating at 70 kHz for 1h at room temperature, rinsing with distilled water, and performing electron microscope scanning on the treated fat tissue, wherein as shown in figure 2, the fat and other impurities in the fat are much less;
then placing the mixture in an octylphenol polyoxyethylene ether solution for extraction for 36h, replacing extract liquor every 12h to remove redundant lipid, repeatedly rinsing the mixture for 4 times by distilled water, centrifuging the mixture for 3 min at 2000g at 4 ℃, repeatedly rinsing the precipitate by distilled water, and carrying out electron microscope scanning on the treated adipose tissues, wherein as shown in figure 3, the fibrous structure of the adipose tissues is shown, and compared with the prior art, the fibrous structures of the adipose tissues are obviously reduced by much grease, and cell structures are not seen;
finally, freeze-drying the tissues by using a vacuum freeze dryer (the temperature is-65 ℃ and the freeze-drying time is 48 hours), preparing adipose tissue epimatrix powder by using a low-temperature grinding method, and directly freezing the powder at-80 ℃ for storage;
the preparation of the emulsifier is as follows: under a vacuum environment, mixing fatty acid methyl ester and water in a mass ratio of 7: 1, heating the mixture to 135 ℃, adding ethylene oxide which is 4 times of the mass of the mixture, uniformly stirring, adding 2 wt% of sodium citrate and gamma-alumina composite catalyst with the mass ratio of 4:6, reacting for 2 hours, cooling to 90 ℃, adding 1 wt% of gamma-alumina catalyst, continuing to react for 1.5 hours, and cooling to normal temperature to obtain a fatty acid methyl ester ethoxylate product;
the octyl phenol polyoxyethylene ether solution is prepared by mixing octyl phenol polyoxyethylene ether with the polymerization degree of 6 and 9 g/L PBS solution, placing the mixture in a water bath at 37 ℃ and stirring for 1.5h, wherein the addition amount of the octyl phenol polyoxyethylene ether is 6 wt% of the PBS solution;
2) preparing sodium alginate into a solution with the concentration of 0.8 mg/mL, magnetically stirring for 4h, adding the adipose tissue extracellular matrix powder prepared in the step 1) to form an adipose extracellular matrix gel with the concentration of 8 mg/mL, uniformly stirring, and standing at 4 ℃ to obtain a tissue mixed solution;
3) adding 2.5% calcium chloride solution into the tissue mixed solution, and stirring to obtain hydrogel.
Example 2
Preparation of an injectable hydrogel for skin repair:
1) preparing an adipose tissue extracellular matrix: taking fat of a human body or a pig, centrifugally dewatering, carrying out tissue crushing treatment on the fat by using a low-temperature tissue grinder under the conditions of 80Hz, 30s and 3 cycles, centrifugally removing water and grease again, carrying out electron microscope scanning on the treated fat tissue, placing the treated fat in liquid nitrogen for 1.5h, rewarming at 35 ℃, circulating for 3 times, then placing the fat in 1M sodium chloride solution, carrying out 80kHz ultrasonic oscillation for 1h at 37 ℃, placing the fat in distilled water for 80kHz ultrasonic oscillation for 1h after rinsing, placing the fat in 5% emulsifier for 1h at room temperature and 80kHz ultrasonic oscillation for 1h after rinsing with distilled water, then rinsing with distilled water, placing the fat in octylphenol polyoxyethylene ether solution for extraction for 28h, replacing extract liquor every 12h to remove redundant lipid, repeatedly rinsing with distilled water for 3 times, centrifuging for 3 min at 2000g at 4 ℃, repeatedly rinsing precipitate with distilled water, finally, freeze-drying the tissues by using a vacuum freeze dryer (the temperature is-70 ℃ and the freeze-drying time is 40 hours), preparing adipose tissue epimatrix powder by using a low-temperature grinding method, and directly freezing the powder at-80 ℃ for storage;
the preparation of the emulsifier is as follows: in a vacuum environment, mixing fatty acid methyl ester and water according to a mass ratio of 8:1, heating to 150 ℃, adding ethylene oxide which is 5 times of the mass of the mixture, uniformly stirring, then adding 3 wt% of sodium citrate and gamma-alumina composite catalyst according to a mass ratio of 5:7, reacting for 2 hours, cooling to 100 ℃, adding 2 wt% of gamma-alumina catalyst, continuing to react for 2 hours, and cooling to the normal temperature to obtain a fatty acid methyl ester ethoxylate product;
the octyl phenol polyoxyethylene ether solution is prepared by mixing octyl phenol polyoxyethylene ether with the polymerization degree of 6 and 10 g/L PBS solution, placing the mixture in a water bath at 40 ℃ and stirring for 1 hour, wherein the addition amount of the octyl phenol polyoxyethylene ether is 7 wt% of the PBS solution;
2) preparing chitosan into a solution with the concentration of 1 mg/mL, magnetically stirring for 5 hours, adding the adipose tissue extracellular matrix powder prepared in the step 1) to form an adipose extracellular matrix gel with the concentration of 10 mg/mL, uniformly stirring, and standing at the temperature of 5 ℃ to obtain a tissue mixed solution;
3) adding 3% calcium chloride solution into the tissue mixed solution, and stirring to obtain hydrogel.
Example 3
Preparation of an injectable hydrogel for skin repair:
1) preparing an adipose tissue extracellular matrix: taking fat of a human body or a pig, centrifugally dewatering, carrying out tissue crushing treatment on the fat by using a low-temperature tissue grinder under the conditions of 65Hz, 40s and 5 cycles, centrifugally removing water and grease again, placing the treated fat in liquid nitrogen for 2 hours, re-warming at 40 ℃, circulating for 4 times, then placing the fat in a 1M sodium chloride solution, carrying out ultrasonic oscillation at 60 kHz for 3 hours at 37 ℃, rinsing with distilled water, placing the fat in distilled water for ultrasonic oscillation at 60 kHz for 1 hour, rinsing with distilled water, placing the fat in a 4% emulsifying agent, carrying out ultrasonic oscillation at 60 kHz for 1 hour at room temperature, rinsing with distilled water, then placing the fat in an octylphenol polyoxyethylene ether solution for extraction for 48 hours, replacing extract liquor every 12 hours to remove redundant lipid, repeatedly rinsing with distilled water for 5 times, centrifuging for 3 minutes at 2000g at 4 ℃, repeatedly rinsing precipitates with distilled water, finally adopting a vacuum freeze dryer (the temperature is-80 ℃, freeze-drying for 36 h), freeze-drying the tissue, preparing the adipose tissue extracellular matrix powder by adopting a low-temperature grinding method, and directly freezing and storing the powder at-80 ℃;
the preparation of the emulsifier is as follows: in a vacuum environment, mixing fatty acid methyl ester and water according to a mass ratio of 6:1, heating to 120 ℃, adding ethylene oxide with the mass being 3 times that of the mixture, uniformly stirring, adding 2 wt% of sodium citrate and gamma-alumina composite catalyst with the mass ratio of 3:4, reacting for 1.5h, cooling to 80 ℃, adding 1 wt% of gamma-alumina catalyst, continuing to react for 1.5h, and cooling to normal temperature to obtain a fatty acid methyl ester ethoxylate product;
the octyl phenol polyoxyethylene ether solution is prepared by mixing octyl phenol polyoxyethylene ether with the polymerization degree of 4 and 8 g/L PBS solution, placing the mixture in a water bath at 35 ℃ and stirring for 2 hours, wherein the addition amount of the octyl phenol polyoxyethylene ether is 5 wt% of the PBS solution;
2) preparing sodium alginate into a solution with the concentration of 0.4 mg/mL, magnetically stirring for 3h, adding the adipose tissue extracellular matrix powder prepared in the step 1) to form an adipose extracellular matrix gel with the concentration of 2 mg/mL, uniformly stirring, and standing at 0 ℃ to obtain a tissue mixed solution;
3) adding a calcium chloride solution with the concentration of 2% into the tissue mixed solution, and uniformly stirring to obtain the hydrogel.
Test examples
8 SD rats (90 g +/-8 g) are divided into two groups, 2 normal growth groups and 6 groups for ultraviolet light aging treatment, and the treatment method comprises the following steps: unhairing the back of rat, treating two square areas of 1cm x 1cm respectively at left and right sides, 2 treating without ultraviolet irradiation, the rest 6 treating with wavelength of 300nm, power of 15W, irradiation height of 80cm, and irradiation energy of 80 mj/cm in the first week2And 160 mj/cm in the second week2The third and fourth sides are 240 mj/cm2. The experiment was started after waiting for another week. The experiment is divided into four groups, the first group is normal rat skin without any intervention, the second group is photoaging rat skin without any intervention, the fourth group is that 100 mu l of the injection gel product is injected into the skin of the rat photoaging skin, the third group is that the other side of the fourth group is injected with physiological saline with the same amount, and after 1 week, 2 weeks and 4 weeks, the thickness of the dermis and the epidermis of the skin is observed by a skin mirror for observation and statistics.
The main cause of the aging behavior of the skin derives from photoaging, while UVB in sunlight plays a crucial role in skin aging-induced wrinkles, mainly manifested by skin roughness, increased wrinkles, formation of age spots and thickening of the irregularities of the stratum corneum. Histologically, uneven thickening of the epidermis and thinning of the dermis are indicated.
As shown in fig. 4 and 5, the skin of the rat was significantly thinned and thickened after 4 weeks of uv irradiation, and gradually recovered after 1 week, 2 weeks, and 4 weeks, but the recovery of the rat was different among different intervention groups. The group that recovered the slowest was the group without any intervention, followed by the physiological saline group, and the fastest was the gel-injected group. It is worth mentioning that there is no difference between the saline injection group and the group without any intervention. Therefore, the injectable gel obtained by combining the fat epimatrix and the hydrogel can greatly improve the photoaging degree of the skin, so that the healthy state of the skin is maintained, and the injectable gel has a repairing effect on the skin.
Claims (9)
1. A method for preparing an injectable hydrogel for skin rejuvenation comprising the steps of:
1) preparing an adipose tissue extracellular matrix: taking animal fat, carrying out centrifugal dehydration, carrying out tissue disruption treatment on the fat, carrying out centrifugation again to remove water and grease, carrying out repeated freeze thawing on the treated fat, then respectively placing the fat in a sodium chloride solution, water and an emulsifier to carry out ultrasonic oscillation treatment, rinsing the fat tissue, then placing the rinsed fat tissue in an octylphenol polyoxyethylene ether solution to extract, centrifuging, cleaning and freeze-drying to obtain fat tissue epimatrix powder;
2) preparing alginate or chitosan into a solution with the concentration of 0.4-1 mg/mL, magnetically stirring for 3-5 hours, adding the adipose tissue extracellular matrix powder prepared in the step 1) to form adipose tissue extracellular matrix glue with the concentration of 2-10 mg/mL, uniformly stirring, and standing at 0-5 ℃ to obtain a tissue mixed solution;
3) adding calcium chloride solution into the tissue mixed solution, and uniformly stirring to obtain the hydrogel.
2. The method for preparing an injectable hydrogel according to claim 1, wherein the step 1) of preparing the adipose tissue extracellular matrix is specifically performed by: taking fat of a human body or a pig, carrying out centrifugal dehydration, carrying out tissue crushing treatment on the fat by using a low-temperature tissue grinder under the conditions of 65-80 Hz, 30-40 s and 3-5 cycles, centrifuging again to remove water and grease, placing the treated fat in liquid nitrogen for 1.5-2 h, rewarming at 35-40 ℃, circulating for 3-4 times, then respectively placing the fat in a sodium chloride solution, water and an emulsifier for ultrasonic oscillation treatment at the frequency of 60-80 kHz for 1-3 h, rinsing the fat tissue, then placing the rinsed fat tissue in an octylphenol polyoxyethylene ether solution for extraction, centrifuging, cleaning and freeze-drying to obtain fat tissue epimatrix powder.
3. The method for preparing an injectable hydrogel according to claim 1, wherein the emulsifier of step 1) is prepared by: in a vacuum environment, mixing fatty acid methyl ester and water, heating to 120-150 ℃, adding ethylene oxide, uniformly stirring, adding a sodium citrate and gamma-alumina composite catalyst, reacting for 1.5-2 h, cooling to 80-100 ℃, adding a gamma-alumina catalyst, continuing to react for 1.5-2 h, and cooling to normal temperature to obtain a fatty acid methyl ester ethoxylate product.
4. The method for preparing an injectable hydrogel according to claim 3, wherein the mixing mass ratio of the fatty acid methyl ester and water in the step 1) is (6-8): 1;
the addition amount of the ethylene oxide is 3-5 times of the mass of the mixture of the fatty acid methyl ester and the water;
the addition amount of the sodium citrate and gamma-alumina composite catalyst is 2-3 wt%, wherein the composite mass ratio of the sodium citrate to the gamma-alumina is (3-5): (4-7);
the addition amount of the gamma-alumina catalyst is 1-2 wt%.
5. The preparation method of the injectable hydrogel of claim 1, wherein the octylphenol polyoxyethylene ether solution in the step 1) is prepared by mixing octylphenol polyoxyethylene ether with 8-10 g/L PBS solution, and stirring in a water bath at 35-40 ℃ for 1-2 h;
the polymerization degree of the octyl phenol polyoxyethylene ether is 4 or 6;
the addition amount of the octyl phenol polyoxyethylene ether is 5-7 wt% of the PBS solution.
6. The method of preparing an injectable hydrogel of claim 1 wherein said alginate of step 2) comprises sodium alginate.
7. The method for preparing an injectable hydrogel according to claim 1, wherein the concentration of calcium chloride in the step 3) is 2 to 3%.
8. An injectable hydrogel prepared by the preparation method of any one of claims 1 to 7 and used for skin repair.
9. Use of the injectable hydrogel of claim 8 for facial filling, wound healing.
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