CN114149919B - Culture device for cassava genetic information transformation - Google Patents
Culture device for cassava genetic information transformation Download PDFInfo
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- CN114149919B CN114149919B CN202111461752.4A CN202111461752A CN114149919B CN 114149919 B CN114149919 B CN 114149919B CN 202111461752 A CN202111461752 A CN 202111461752A CN 114149919 B CN114149919 B CN 114149919B
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Abstract
The invention discloses a culture device for cassava genetic information transformation, which comprises the following components: the box body is provided with a first motor on the outer surface of the left panel; the U-shaped plate is connected with the first motor through a rotating shaft and is connected with the loading frame through a stand column; the loading frame is connected with mounting seats at two ends; the gear is sleeved on the transmission shaft, the gear is meshed with the external teeth, and one end of the transmission shaft is connected with the second motor; the sliding block is arranged on the sliding rail, and a side plate is fixed on the top surface of the sliding block; and the bottle mouth of the culture bottle stretches into the mounting seat. This a culture apparatus for cassava genetic information conversion utilizes mount pad intercommunication upper and lower two blake bottles, makes the position of two blake bottles reverse through upset loading frame, and the liquid in the upside blake bottle flows into the downside blake bottle, conveniently changes the solution of soaking the cassava, avoids taking out the cassava of blake bottle, reduces the risk that the cassava receives external interference.
Description
Technical Field
The invention relates to the technical field related to genetic engineering, in particular to a culture device for cassava genetic information transformation.
Background
Cassava is a plant of the genus cassava of the family Euphorbiaceae, drought-resistant and barren-resistant, is one of three tuber crops, and the sixth food crop worldwide, called "starch king", is a ration for nearly six hundred million people worldwide.
The plant genetic transformation technology is also called a plant transgenic technology, and mainly depends on a specific mode to introduce exogenous genes into a receptor plant, and can carry out stable inheritance, at present, common cassava genetic information transformation is that agrobacterium is adopted to infect cassava callus, the cassava callus needs to be soaked and shaken in invasive dye liquor for 20 minutes in the process, then the cassava callus is taken out to be subjected to darkroom culture for 48 hours, then the cassava callus is added into sterile water to be subjected to degerming treatment, and finally the cassava callus is added into a screening culture medium to be subjected to darkroom culture for one month.
Disclosure of Invention
The invention provides a culture device for cassava genetic information transformation in order to make up market blank.
The invention aims to provide a culture device for cassava genetic information transformation, which aims to solve the problems that when the cassava callus needs to be transferred back and forth in the prior art, the time required by the cassava genetic transformation is increased, and the risk that the cassava callus is interfered by external bacteria is increased.
In order to achieve the above purpose, the present invention provides the following technical solutions: a culture device for the transformation of genetic information of cassava, comprising:
the box body is characterized in that a first motor is arranged on the outer surface of a left panel of the box body, and a sliding rail is arranged on the inner wall of a bottom plate of the box body;
the U-shaped plate is connected with the first motor through a rotating shaft and is connected with the loading frame through a stand column;
the device comprises a loading frame, wherein both ends of the loading frame are connected with mounting seats, heating rings, partition plates and grid rods are arranged in the mounting seats, and screw holes are formed in the upper end and the lower end of the mounting seats;
the gear is sleeved on the transmission shaft, the gear is meshed with the external teeth, and one end of the transmission shaft is connected with the second motor;
the sliding block is arranged on the sliding rail, a side plate is fixed on the top surface of the sliding block, and a heating wire and a lamp bead are embedded on the side plate;
the bottle mouth of the culture bottle stretches into the mounting seat, and the side wall of the culture bottle is provided with a ventilation plug.
Further, the pivot runs through the left panel of box, the both ends of pivot connect respectively the U-shaped board with a motor, a motor is used for the drive U-shaped board rotates, the interval between two boards is greater than about the U-shaped board the thickness of loading frame.
Further, the bottom of stand is fixed on the bottom plate of U-shaped board, the stand wears out the roof connection roof of U-shaped board, the roof with the transmission shaft links to each other, the bottom of transmission shaft runs through gear connection the second motor, the second motor is fixed on the bottom plate of U-shaped board, the second motor is used for the drive gear rotation.
Further, the external teeth are arranged on the outer arc surface of the loading frame, a protruding block is arranged in the middle of the inner arc surface of the loading frame, a jack is formed in the protruding block, and the upright post is inserted into the jack.
Further, a partition plate is horizontally fixed in the middle of the inner wall of the mounting seat, the center of the partition plate is provided with a grid rod, the upper end and the lower end of the grid rod respectively extend into the corresponding screw holes, and heating rings are symmetrically arranged in the mounting seat on the upper side and the lower side of the partition plate.
Further, the radius of the inner ring of the heating ring is larger than that of the mounting seat, an electrode column is mounted on the heating ring, the electrode column stretches into the limiting groove, an electrode plate is mounted in the limiting groove, and the electrode column is matched with the electrode plate.
Further, the bottom of slider has seted up the spout, the through-hole has been seted up on the roof of slider, install the fan in the through-hole, both sides around the through-hole symmetrical install on the slider the curb plate, the interval between the curb plate opposite surface is greater than the diameter of blake bottle.
Further, the heating wire is used for providing culture temperature, the lamp beads are used for providing culture illumination, and the heating wire and the lamp beads are uniformly distributed on the side plates.
Further, the ventilation plug is close to the bottle opening, and the bottle opening is matched with the screw hole.
Compared with the prior art, the invention has the beneficial effects that: the culture device for cassava genetic information conversion is reasonable in structural arrangement, the upper culture flask and the lower culture flask are communicated by the mounting seat, the positions of the two culture flasks are reversed by overturning the loading frame, liquid in the upper culture flask flows into the lower culture flask, the solution for soaking the cassava is convenient to replace, the cassava of the culture flask is prevented from being taken out, the risk of the cassava being interfered by the outside is reduced, the bottleneck of the culture flask loaded each time is disinfected by the heating ring, the burden of operators is reduced, the slide block is used for driving the side plate to move, and a proper culture environment is provided for the culture flask;
1. screw holes which are communicated up and down are formed in the mounting seat, so that two culture bottles on the same mounting seat are conveniently communicated with each other, and a partition plate and a grid rod are arranged in the mounting seat, so that liquid in the culture bottles can be transferred through the mounting seat, solids are reserved in the culture bottles, and the exposure times of cassava are reduced;
2. the gear is meshed with the external teeth, the second motor drives the gear to rotate so that the loading frame shakes back and forth, the shaking effect is realized, and the transfer of the culture bottle into shaking table equipment is avoided;
3. the side plate and the fan on the slide block move along the slide rail along with the slide block, so that the side plate and the fan move around the culture bottle, the temperature and the illumination condition around the culture bottle are adjusted, and the transfer of the culture bottle into darkroom equipment is avoided;
4. the upper side and the lower side of the partition plate are provided with heating rings, when the culture flask is not installed in the screw hole on the lower side, the heating rings on the lower side leave the partition plate under the action of gravity, electrode columns on the heating rings penetrate into the limit grooves to be connected with the electrode plates, the heating rings are powered on to heat, the bottle mouth is filled into the screw hole at the moment, the bottle mouth contacts with the high-temperature heating rings to achieve a sterilization effect, and meanwhile, the bottle mouth jacks up the heating rings upwards to separate the electrode columns from the electrode plates, so that the power-off effect is achieved.
Drawings
FIG. 1 is a schematic top view of a structure of the present invention;
FIG. 2 is a schematic side cross-sectional view of the structure of the present invention;
FIG. 3 is an enlarged schematic view of the structure of the present invention in section A of FIG. 2;
FIG. 4 is a schematic elevational cross-section of the structure of the present invention;
FIG. 5 is a schematic view of a carrier of the present invention;
FIG. 6 is a schematic view of a slider of the present invention;
FIG. 7 is a schematic view of a heating ring of the present invention.
In the figure: 1. a case; 2. a U-shaped plate; 3. a loading rack; 4. a gear; 5. a slide block; 6. a culture bottle; 21. A rotating shaft; 22. a first motor; 23. a column; 31. external teeth; 32. a mounting base; 33. a bump; 331. A jack; 321. a screw hole; 322. a heating ring; 323. a partition plate; 324. grid rods; 325. a limit groove; 326. an electrode sheet; 327. an electrode column; 41. a top plate; 42. a second motor; 43. a transmission shaft; 51. A side plate; 52. a blower; 53. a slide rail; 54. a through hole; 55. a heating wire; 56. a lamp bead; 57. a chute; 61. a vent plug; 62. and (3) a bottle opening.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The first embodiment is as follows: referring to fig. 1-7, the present invention provides a technical solution: a culture device for the transformation of genetic information of cassava, comprising:
the box body 1, the first motor 22 is arranged on the outer surface of the left panel of the box body 1, and the sliding rail 53 is arranged on the inner wall of the bottom plate of the box body 1;
the U-shaped plate 2 is connected with the first motor 22 through a rotating shaft 21, and the U-shaped plate 2 is connected with the loading frame 3 through a stand column 23;
the loading frame 3, both ends of the loading frame 3 are connected with mounting seats 32, heating rings 322, partition plates 323 and grid rods 324 are arranged in the mounting seats 32, and screw holes 321 are formed in the upper end and the lower end of the mounting seats 32;
the gear 4 is sleeved on the transmission shaft 43, the gear 4 is meshed with the external teeth 31, and one end of the transmission shaft 43 is connected with the second motor 42;
the sliding block 5 is arranged on the sliding rail 53, a side plate 51 is fixed on the top surface of the sliding block 5, and a heating wire 55 and a lamp bead 56 are embedded on the side plate 51;
the bottle mouth 62 of the culture bottle 6 stretches into the mounting seat 32, and the side wall of the culture bottle 6 is provided with a ventilation plug 61.
When the culture device for cassava genetic information conversion is used, cassava callus is added into a culture flask 6, a box body 1 is opened to enable a bottleneck 62 of the culture flask 6 to be connected to an installation seat 32 in a screwed mode, a first motor 22 is started to drive a rotating shaft 21 to rotate, a U-shaped plate 2 and a loading frame 3 are enabled to be turned over 180 degrees, the culture flask 6 filled with the invasion solution is connected to the same installation seat 32 in a screwed mode, the U-shaped plate 2 and the loading frame 3 are turned over again, the invasion solution flows into the cassava culture flask 6 through a grid rod 324, a second motor 42 is started to drive a transmission shaft 43 to rotate, the transmission shaft 43 drives a gear 4 to rotate, the gear 4 drives the loading frame 3 to rotate around a stand 23, the effect of shaking the culture flask 6 is achieved, after infection is completed, the invasion solution is separated from the cassava, the invasion solution culture flask 6 is taken down, the culture flask 6 filled with sterile water is turned over after a period of time, the sterile water is enabled to enter the culture flask 6, the operation is repeated, the sterilization treatment is carried out, the culture flask 6 filled with the culture solution after the sterile water is taken down, the culture flask 6 is enabled to flow into the cassava culture flask 6, the culture flask 6 is enabled to move along a slide rail 5, and the slide rails are enabled to move around the cassava flask 6.
The second embodiment is as follows: in this embodiment, as shown in fig. 1 and fig. 4, the rotating shaft 21 penetrates through the left panel of the box body 1, two ends of the rotating shaft 21 are respectively connected with the U-shaped plate 2 and the first motor 22, the first motor 22 is used for driving the U-shaped plate 2 to rotate, the distance between the upper plate and the lower plate of the U-shaped plate 2 is larger than the thickness of the loading frame 3, the U-shaped plate 2 is hinged with the loading frame 3, and when the first motor 22 drives the U-shaped plate 2 to turn over, the loading frame 3 also turns over.
And a third specific embodiment: this embodiment is further limited in the first embodiment, as shown in fig. 1, fig. 4 and fig. 5, the bottom end of the upright 23 is fixed on the bottom plate of the U-shaped plate 2, the upright 23 penetrates out of the top plate 41 of the U-shaped plate 2 to connect with the top plate 41, the top plate 41 is connected with the transmission shaft 43, the bottom end of the transmission shaft 43 penetrates through the gear 4 to connect with the second motor 42, the second motor 42 is fixed on the bottom plate of the U-shaped plate 2, the second motor 42 is used for driving the gear 4 to rotate, when the second motor 42 drives the gear 4 to rotate, the loading frame 3 rotates around the upright 23, so as to realize shaking effect, and the substances of the culture flask 6 are convenient to mix uniformly.
The specific embodiment IV is as follows: in this embodiment, as shown in fig. 1 and 5, external teeth 31 are disposed on the outer arc surface of the loading frame 3, a bump 33 is disposed in the middle of the inner arc surface of the loading frame 3, a jack 331 is disposed on the bump 33, and a column 23 is inserted into the jack 331.
Fifth embodiment: in this embodiment, as shown in fig. 2 and 3, a partition plate 323 is horizontally fixed in the middle of the inner wall of the mounting seat 32, a grid rod 324 is installed at the center of the partition plate 323, the upper and lower ends of the grid rod 324 respectively extend into corresponding screw holes 321, heating rings 322 are symmetrically arranged in the mounting seat 32 on the upper and lower sides of the partition plate 323, the grid rod 324 has a filtering effect, can accelerate the flow of liquid, has a guiding function, and prevents the liquid from flowing away.
Specific embodiment six: in this embodiment, as the fifth embodiment is further defined, the radius of the inner ring of the heating ring 322 is larger than the radius of the mounting seat 32, the electrode column 327 is mounted on the heating ring 322, the electrode column 327 extends into the limit groove 325, the electrode plate 326 is mounted in the limit groove 325, the electrode column 327 is matched with the electrode plate 326, the heating ring 322 falls down under the action of gravity, the electrode column 327 on the heating ring 322 is contacted with the electrode plate 326, so that the heating ring 322 is electrified for sterilization, when the flask 6 is mounted, the electrode column 327 is separated from the electrode plate 326 when the flask mouth 62 jacks up the heating ring 322, and the heating ring 322 stops heating.
Seventh embodiment: in this embodiment, for further limitation of the first embodiment, a chute 57 is formed at the bottom of the slider 5, a through hole 54 is formed in the top plate 41 of the slider 5, a fan 52 is installed in the through hole 54, side plates 51 are symmetrically installed on the sliders 5 on the front side and the rear side of the through hole 54, the distance between opposite surfaces of the side plates 51 is larger than the diameter of the culture flask 6, and the sliders 5 and the sliding rails 53 are arranged to prevent the culture flask 6 from being turned over and only move around the culture flask 6 when needed.
Eighth embodiment: in this embodiment, as shown in fig. 1 and fig. 6, the heating wire 55 is used for providing a culture temperature, the lamp beads 56 are used for providing a culture light, the heating wire 55 and the lamp beads 56 are uniformly distributed on the side plate 51, the requirements of different culture stages on the environment are different, the environmental conditions are flexibly adjusted through the heating wire 55 and the lamp beads 56, and the requirement of cassava growth is met.
Detailed description nine: in this embodiment, as shown in fig. 2 and 3, the ventilation plug 61 is close to the bottle mouth 62, the bottle mouth 62 is matched with the screw hole 321, the ventilation plug 61 is convenient to release gas or provide oxygen according to requirements in the culturing process, a proper culturing environment is constructed, and the screw hole 321 is arranged to facilitate loading and unloading of the culture flask 6.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A culture device for the transformation of genetic information of cassava, comprising:
the box body (1), the first motor (22) is arranged on the outer surface of the left panel of the box body (1), and the sliding rail (53) is arranged on the inner wall of the bottom plate of the box body (1);
the U-shaped plate (2), U-shaped plate (2) are connected with first motor (22) through pivot (21), U-shaped plate (2) are connected with loading frame (3) through stand (23), pivot (21) run through the left panel of box (1), the both ends of pivot (21) are connected respectively U-shaped plate (2) with first motor (22), first motor (22) are used for driving U-shaped plate (2) rotate, the interval between two upper and lower plates of U-shaped plate (2) is greater than loading frame (3) thickness;
the device comprises a loading frame (3), wherein external teeth (31) are arranged on an outer cambered surface of the loading frame (3), mounting seats (32) are respectively connected to two ends of the loading frame (3), heating rings (322), partition plates (323) and grid rods (324) are arranged in the mounting seats (32), screw holes (321) are respectively formed in the upper end and the lower end of each mounting seat (32), the partition plates (323) are horizontally fixed to the middle part of the inner wall of each mounting seat (32), grid rods (324) are arranged at the center of each partition plate (323), the upper end and the lower end of each grid rod (324) respectively extend into the corresponding screw holes (321), and the heating rings (322) are symmetrically arranged in the mounting seats (32) on the upper side and the lower side of each partition plate (323);
the gear (4) is sleeved on the transmission shaft (43), the gear (4) is meshed with the external teeth (31), and one end of the transmission shaft (43) is connected with the second motor (42);
the sliding block (5) is arranged on the sliding rail (53), a side plate (51) is fixed on the top surface of the sliding block (5), and a heating wire (55) and a lamp bead (56) are embedded on the side plate (51);
a bottle mouth (62) of the culture bottle (6) stretches into the mounting seat (32), and a ventilation plug (61) is arranged on the side wall of the culture bottle (6);
the inner ring radius of the heating ring (322) is larger than the radius of the mounting seat (32), an electrode column (327) is mounted on the heating ring (322), the electrode column (327) stretches into the limiting groove (325), an electrode plate (326) is mounted in the limiting groove (325), and the electrode column (327) is matched with the electrode plate (326).
2. The culture device for cassava genetic information transformation according to claim 1, in which: the bottom of stand (23) is fixed on the bottom plate of U-shaped board (2), stand (23) wear out roof connection roof (41) of U-shaped board (2), roof (41) with transmission shaft (43) link to each other, the bottom of transmission shaft (43) runs through gear (4) are connected second motor (42), second motor (42) are fixed on the bottom plate of U-shaped board (2), second motor (42) are used for the drive gear (4) rotate.
3. The culture device for cassava genetic information transformation according to claim 1, in which: the middle part of the intrados of the loading frame (3) is provided with a lug (33), the lug (33) is provided with a jack (331), and the upright post (23) is inserted into the jack (331).
4. The culture device for cassava genetic information transformation according to claim 1, in which: the bottom of slider (5) has seted up spout (57), through-hole (54) have been seted up on the roof of slider (5), install fan (52) in through-hole (54), both sides around through-hole (54) on slider (5) symmetry install curb plate (51), the interval between the opposite face of curb plate (51) is greater than the diameter of blake bottle (6).
5. The culture device for cassava genetic information transformation according to claim 1, in which: the heating wire (55) is used for providing culture temperature, the lamp beads (56) are used for providing culture illumination, and the heating wire (55) and the lamp beads (56) are uniformly distributed on the side plate (51).
6. The culture device for cassava genetic information transformation according to claim 1, in which: the vent plug (61) is close to the bottle opening (62), and the bottle opening (62) is matched with the screw hole (321).
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