CN114097802B - Emulsifiable concentrate containing epoxiconazole and isoprothiolane - Google Patents

Abstract

The invention provides an emulsifiable concentrate containing epoxiconazole and isoprothiolane, wherein the weight ratio of epoxiconazole to isoprothiolane is 1. The emulsifiable concentrate containing epoxiconazole and isoprothiolane is used for preventing and treating rice sheath blight disease, rice blast, false smut, corn northern leaf spot and corn rust disease.

Description

Emulsifiable concentrate containing epoxiconazole and isoprothiolane

The application is a divisional application with the application number of 201811290158.1 (the application date of the original application is 2018, 10 and 31 days, and the invention name is a bactericidal composition).

Technical Field

The invention relates to the field of pesticide chemistry, in particular to an emulsifiable concentrate containing epoxiconazole and isoprothiolane and application of the emulsifiable concentrate containing epoxiconazole and isoprothiolane in preventing and treating plant pathogenic fungi from infecting plants in the field of agriculture or horticulture.

Background

With respect to the activity of pesticides, in particular the protection of crops, one of the core problems of the research developed in this field is to improve the properties, in particular in terms of biological activity, and to maintain this activity over a certain period of time.

Epoxiconazole (Epoxiconazole), known by the chemical name (2RS, 3RS) -1- [3- (2-chlorophenyl) -2, 3-epoxy-2- (4-fluorophenyl) propyl ] -1-hydro-1, 2, 4-triazole, is known as a fungicide from EP 94564. The structural formula is as follows:

isoprothiolane (Isoprothiolane), which has a chemical name of 1, 3-dithiopentane-2-methylene malonic acid diisopropyl ester, belongs to a systemic fungicide and has a special effect on rice blast.

CN102273442A discloses an ultralow volume liquid containing epoxiconazole, which takes epoxiconazole or a compound of epoxiconazole and an active component II as an active component, and an auxiliary agent and a solvent are added to prepare the ultralow volume liquid; wherein the active component II can be selected from isoprothiolane. Although it discloses a combination of epoxiconazole and isoprothiolane, it discloses that a superior bactericidal effect to that of epoxiconazole emulsifiable concentrate single dose is achieved only when the ratio of the two is in the range of 1.

Therefore, how to expand the preparation forms of epoxiconazole and isoprothiolane and to make the epoxiconazole and isoprothiolane prepared in a certain proportion show more excellent effects in sterilization is a problem to be solved at present.

Disclosure of Invention

The object of the present invention is to provide a fungicidal composition having improved activity against harmful fungi at a reduced total amount of active compounds applied, and exerting a synergistic effect in combination, in terms of a reduced application rate and an improved spectrum of activity of known compounds epoxiconazole and isoprothiolane.

The present inventors have found that the simultaneous, i.e. joint or separate, application of epoxiconazole and isoprothiolane, or the sequential application of epoxiconazole and isoprothiolane, allows better control of harmful fungi than the individual compounds applied individually.

The invention provides a bactericidal composition, which is prepared by binary compounding of epoxiconazole and isoprothiolane, so that the obtained composition has a gain effect on the prevention and treatment effects, the bactericidal spectrum is expanded, the effect of one medicine for multiple purposes is achieved, and the generation of drug resistance of germs can be effectively slowed down or avoided. Surprisingly, the fungicidal activity of the fungicidal compositions according to the invention is significantly higher than the sum of the activities of the individual active compounds. In other words, there is an unpredictable, truly present synergistic effect of the combination of the two, not just a supplementation of the activity.

The inventor finds that the sterilization composition containing epoxiconazole and isoprothiolane can control the fungal and bacterial diseases of various vegetables, fruits, fruit trees and cereal crops through various intensive researches. The composition containing epoxiconazole and isoprothiolane is very effective not only against common plant pathogens but also against those pathogens which have developed resistance to drugs, and has excellent control effects even under conditions where diseases continue to develop, and thus the present invention has been completed.

The synergistic effect is particularly pronounced when the active compounds are present in the fungicidal compositions of the present invention in a particular weight ratio.

The bactericidal composition provided by the invention has a synergistic effect and is suitable for preventing and treating harmful fungi and bacteria. And the bactericidal composition is especially suitable for controlling fungal and bacterial diseases in cereals, vegetables, fruits, ornamental plants and grapevines.

The invention provides a sterilization composition which is realized by adopting the following technical scheme:

a sterilization composition comprises active ingredients of epoxiconazole and isoprothiolane, wherein the weight ratio of the epoxiconazole to the isoprothiolane is 1.

Epoxiconazole and isoprothiolane may be applied simultaneously, i.e. together or separately, or sequentially; the sequence in the case of separate application generally has no effect on the results of the control measures.

The weight ratio of the active ingredients of epoxiconazole and isoprothiolane can be, for example, 1.

The bactericidal composition comprises 12% -90%, preferably 12% -80%, more preferably 12% -60%, and more preferably 12% -50% of active ingredients, namely epoxiconazole and isoprothiolane.

The bactericidal composition can also comprise 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90% of the sum of the active ingredients, namely epoxiconazole and isoprothiolane.

In particular, in the bactericidal composition, the mass of the active ingredient epoxiconazole accounts for 2% -15% of the bactericidal composition, and can be, for example, 2%, 3%, 5%, 8%, 10%, 12%, 15%; the isoprothiolane accounts for 18-75% of the bactericidal composition by mass, such as 18%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%.

In particular, in the bactericidal composition, the mass of the active ingredient epoxiconazole accounts for 5% -15% of the bactericidal composition; the isoprothiolane accounts for 20-50% of the bactericidal composition by mass.

The bactericidal composition can be prepared into various dosage forms, such as wettable powder, missible oil, suspending agent, suspending emulsion, microcapsule, seed coating agent, microemulsion, aqueous emulsion, water dispersible granule, foaming agent, paste, aerosol or ultra-low volume spray liquid, particularly preferably missible oil, aqueous emulsion and suspending agent, and most preferably missible oil.

The bactericidal composition may contain only the active ingredient, or may further contain a filler and/or a surfactant.

According to the invention, the term "filler" refers to a natural or synthetic organic or inorganic compound that can be combined or associated with an active compound to make it easier to apply to a subject (e.g. plants, crops or grasses). Thus, the filler is preferably inert, at least should be agriculturally acceptable. The filler may be solid or liquid.

Liquid fillers are typically: water, alcohols (e.g., methanol, ethanol, isopropanol, butanol, ethylene glycol, etc.), ketones (e.g., acetone, methyl ethyl ketone, diisobutyl ketone, cyclohexanone, etc.), ethers (e.g., diethyl ether, dioxane, methyl cellulose, tetrahydrofuran, etc.), aliphatic hydrocarbons (e.g., kerosene, mineral oil, etc.), aromatic hydrocarbons (e.g., benzene, toluene, xylene, mineral spirits, alkylnaphthalenes, chlorinated aromatic hydrocarbons, chlorinated aliphatic hydrocarbons, chlorobenzene, etc.), halogenated hydrocarbons, amides, sulfones, dimethyl sulfoxide, mineral and vegetable oils, animal oils, etc.

Solid fillers are typically: examples of the inorganic mineral powder include plant powders (for example, soybean powder, starch, cereal powder, wood powder, bark powder, saw dust, walnut shell powder, bran, cellulose powder, coconut shell, particles of corn cob and tobacco stalk, residues after extraction of plant essence, and the like), synthetic polymers such as paper, saw dust, ground synthetic resins, clays (for example, kaolin, bentonite, acid china clay, and the like), talc powders, silicas (for example, diatomaceous earth, silica sand, mica, hydrous silicic acid, and calcium silicate), activated carbon, natural minerals (pumice, attapulgite, zeolite, and the like), calcined diatomaceous earth, sand, plastic media (for example, polyethylene, polypropylene, polyvinylidene chloride, and the like), inorganic mineral powders such as potassium chloride, calcium carbonate, and calcium phosphate, chemical fertilizers such as ammonium sulfate, ammonium phosphate, urea, and ammonium chloride, and clay fertilizers, and these may be used alone or in combination of 2 or more.

Examples of the surfactant that can be used for emulsifying, dispersing, solubilizing, and/or wetting the active ingredient compound include polyacrylic acid salts such as fatty alcohol polyoxyethylene ether, polyoxyethylene alkylaryl ether, polyoxyethylene higher fatty acid ester, phosphoric acid ester of polyoxyethylene alcohol or phenol, fatty acid ester of polyhydric alcohol, alkylaryl sulfonic acid, naphthalenesulfonic acid polymer, lignosulfonate, branched polymer of high molecular comb, butylnaphthalenesulfonate, alkylaryl sulfonate, sodium alkylsulfosuccinate, fats and oils, condensates of fatty alcohol and ethylene oxide, and alkyltaurates, and protein hydrolysates. Suitable oligosaccharides or polymers are based, for example, on ethylene monomers, acrylic acid, polyoxyethylene and/or polyoxypropylene alone or in combination with, for example, (poly) alcohols or (poly) amines.

Tackifiers such as carboxymethylcellulose and natural and synthetic polymers in the form of powders, granules or latices, such as gum arabic, polyvinyl alcohol and polyvinyl acetate, or else natural phospholipids, such as cephalins and lecithins, and synthetic phospholipids, can also be used in the formulations. Other additives are mineral oils and vegetable oils.

Colorants which may be used are, for example, inorganic pigments, such as iron oxide, titanium oxide and Prussian blue, and organic dyes, such as alizarin dyes, azo dyes or metal phthalocyanine dyes, and trace nutrients, such as salts of iron, manganese, boron, ketones, cobalt, molybdenum and zinc.

Disintegrants which may be used are selected from: one or more of bentonite, urea, ammonium sulfate, aluminum chloride, citric acid, succinic acid and sodium bicarbonate.

Stabilizers which may be used are selected from: one of sodium citrate and resorcinol.

The antifreeze agents which may be used are selected from: one or more of ethylene glycol, propylene glycol, glycerol and urea.

The defoaming agent is selected from: silicone oil, silicone compound, C10-20 saturated fatty acid compound, and C8-10 fatty alcohol compound.

Optionally, further additional components, such as protective colloids, binders, thickeners, thixotropic agents, penetrating agents, stabilizers, masking agents, can also be included.

The formulations of the invention can be prepared by mixing the active ingredients with fillers and/or surfactants in a known manner, it being possible for further conventional additives, such as siccatives, colorants, stabilizers, pigments, defoamers, preservatives, thickeners, etc., to be added.

The fungicidal composition of the present invention has a strong activity against various plant pathogenic bacteria, and can exert a strong control effect on the prevention and treatment of plant diseases caused by plant pathogenic bacteria.

The bactericidal composition is used for preventing and controlling fungi and bacteria on cereals, vegetables, fruits, ornamental plants and grapevines.

The fungicidal composition of the present invention has excellent activity against a wide range of phytopathogenic fungi such as basidiomycetes, ascomycetes, oomycetes and deuteromycetes.

Oomycetes, including Phytophthora (Phytophthora), such as diseases of Phytophthora infestans (Phytophthora sojae), phytophthora sojae (Phytophthora sojae), phytophthora citri (Phytophorapapraparitica), phytophthora camphora (Phytophthora cinnnamimomici) and Phytophthora squamosa (Phytophthora capsici); pythium species (Pythium), such as Pythium turf blight (Pythium aphanidermatum); and diseases of the Peronosporaceae family (Peronosporaceae), such as peronospora viticola (plasmopara); peronospora species (Peronospora) (including Peronospora tabacum (peronosporaabacina) and Peronospora parasitica (Peronospora paralica)); pseudoperonospora (Pseudoperonospora) pathogens (including Pseudoperonospora cubensis (Pseudoperonospora cubensis) and Bremia lactucae (Bremia lactucae)); pythium species (Pythium) such as Pythium aphanidermatum (Pythium aphanidermatum); plasmopara.

Ascomycetes, including Alternaria (Alternaria) diseases, such as tomato early blight (Alternaria) and cabbage black spot (Alternaria), mycosphaeremopsis (Guignardia) diseases, such as grape black rot (Guignardia bidwelli), venturia (Venturia) diseases, such as apple scab (Venturia) diseases, septoria (Septoria) diseases, such as Septoria nodorum (Septorium) and leaf blight (Septoria), powdery mildew, such as Erysiphe (Erysiphe) (including wheat powdery mildew (Erysiphe) and gloiophyllum (Erysiphe)), grape powdery mildew (Uncinula nerium), powdery mildew (Sphaerothecium powdery mildew) and apple powdery mildew (Pouteria), and Sphaerophyllum fructicola (Pseudocera), botrytis (Botrytis) species diseases such as Botrytis cinerea (Botrytis cinerea), monilinia persica (monilini fructicola) diseases, sclerotinia (Sclerotinia) species diseases, such as Sclerotinia sclerotiorum (Sclerotinia sclerotiorum), pyricularia oryzae (magnaporthia), botrytis cinerea (phomopsis viticola) diseases, helminthosporium (Helminthosporium) species diseases such as northern leaf blight (Helminthosporium), pyricularia reticulata (pyrenophora) species diseases, anthrax diseases such as gliosis (glomeridia nigra) or Colletotrichum (Colletotrichum) diseases (such as Colletotrichum anthracnose (colletotricholicola) and citrullaria citrulli)); wheat take-all (gaeumannomyces graminis); sphaerotheca (Podosphaera), sclerotinia (Monilinia), uncinula (Uncinula), and Mycosphaerella (Mycosphaerella).

Basidiomycetes include rust diseases caused by the genus Ruscus (Puccinia) (e.g., pucciniarecondita (Pucciniarecondita), pucciniastriiformis (Pucciniastriiformis), puccinia (Pucciniahordei), puccinia (Pucciniagraminis), and Puccinia (Pucciniaarachidis), coffee rust (Hemilavastriotrix), camellia rust (Hemeia), soybean rust (Phakopsora hygizi); ustilago (Llstiarginalcs).

Deuteromycetes, including Rhizoctonia species (Rhizoctonia species) (e.g., rhizoctonia solani (Rhizoctonia) and Rhizoctonia rubescens (Rhizoctonia oryzae), fusarium (Fusarium) diseases such as Fusarium graminearum (Fusarium graminearum), fusarium candidum (Fusarium moniliforme), fusarium oxysporum (Fusarium oxysporum), fusarium moniliforme (Fusarium proliferatum), fusarium solani (Fusarium solani), verticillium dahle (Verticillium dahle), rhizoctonia sclerotiorum (Sclerotinfsii), phyllospora reticulata (Rhizoctonia cerealis), ceriporiospora nigra (Cercospora nigra), pyrophora (Pyrococcus), pyrococcus Alternaria and Pyrococcus (Fusarium), fusarium solani (Cercosporella), fusarium (Cercosporella) and Fusarium (Cercosporella).

The fungicidal compositions of the present invention are particularly effective against the following classes of phytopathogenic fungi: botrytis (Botrytis), pyricularia (Pyricularia), helminthosporium (Helminthosporium), sclerotinia (Sclerotinia), fusarium (Fusarium), septoria (Septoria), cercospora (Cercospora), alternaria (Alternaria), pyricularia (Pyricularia), pseudocercospora (Pseudocercospora), rhizoctonia (Rhizoctonia), puccinia (Hemileia), puccinia (Puccinia) Phakopsora (Phakopsora), ustilago (lstillagina), venturia (Venturia), erysiphe (Erysiphe), sphaerotheca (podosphaea), sclerotinia (Monilinia), leptospira (Uncinula), mycosphaerella (mycosphaeruella), phytophthora (Phytophthora), pythium (Pythium), plasmopara (Plasmopara), peronospora (Peronospora), pseudoperonospora (Pseudoperonospora), and stemphylium (bremia).

The fungicidal compositions of the present invention are suitable for crop plants consisting essentially of: cereal crops such as wheat, barley, oats, rye, triticale, rice, maize, sorghum and millet; vine crops such as fresh grapes and wine grapes; field crops, such as rape (canola), sunflower; sugar beet, sugar cane, soybean, peanut (groundnut), tobacco, alfalfa, clover, lespedeza, clover, and vetch; pome fruits such as apple, pear, wild apple, loquat, hawthorn and quince; stone fruit, such as peach, cherry, plum, apricot, nectarine; citrus fruits such as lemon, lime, orange, grapefruit, mandarin orange (tangerine), and kumquat; rhizome plants and field crops (and their leaves), such as artichoke, beet and sugar beet, carrot, cassava, ginger, ginseng, horseradish, parsnip, potato, radish, turnip cabbage, sweet potato, turnip and yam; bulb plants such as garlic, leek, onion and shallot; leafy vegetable plants, such as mustard (sesamol), celery, cress, chicory (cogongrass), fennel, lettuce head and lettuce loose leaves, parsley, red chicory, rhubarb, spinach and swiss chard; brassica (Korean) leafy vegetables such as broccoli, cauliflower (broccoli), brussels sprouts, cabbage, chinese cabbage, cauliflower, cabbage, kale, kohlrabi, mustard and green vegetables; legume plants (succulent or juicless) such as lupins, beans) (including broad beans, kidney beans, flower beans, safflower beans, pod beans, broadleaf beans), beans (including adzuki beans, asparagus beans, brow beans, black cowpeas, vigna beans, vigna radiata, cowpeas, black beans, and ultralong cowpeas), broad beans, chickpeas, guar ears, jack beans, lentils, and peas (including kidney beans, pod peas, purple peas, green peas, snow beans, sweet beans, pigeon peas, and soybeans); fruit vegetables such as eggplant, cherries, cantaloupe, and capsicum (including bell pepper, capsicum, culinary capsicum, capsicum annuum, pimento; tomato, and tomato); cucurbitaceous vegetables such as chayote (fruit), wax gourd, citrullus lanatus, cucumber, young cucumber, edible cucurbits (including cucurbits, calabash), luffa, okra, gummy balsam pear, charantin, balsam pear, and chinese cucumber, muskmelon, zucchini, squash and watermelon; berries, such as blackberry, red berry, dewberry, purple blueberry, cranberry, blackcurrant, wild berry, loganberry, raspberry and strawberry; tree nuts such as almonds, beech nuts, brazil nuts, walnuts, cashews, chestnuts, hazelnuts (hazelnuts), pecans, macadamia nuts, pecans, and walnuts; tropical fruits and other crops, such as bananas, plantains, mangos, coconut, papaya, avocados, lychees, agave, coffee, cocoa, sugar cane, oil palm, sesame, gums and spices; fiber crops, such as cotton, flax and hemp; turf grass (including warm-season and cool-season turf grass).

Preferably, crop plants to which the fungicidal compositions of the present invention are suitable generally include plants of the following genera: cereals (wheat, barley, rye, oats, rice, maize, sorghum and related varieties); sugar beet (sugar beet and fodder beet); pomes, stone fruits and berries (apples, pears, plums, peaches, apricots, cherries, strawberries, raspberries and blackberries); leguminous plants (beans, squash beans, peas, soybeans); plants of the oil family (rape, mustard, poppy, olive, sunflower, coconut, castor, cocoa beans, groundnut); cucurbits (squash, cucumber, melon); fiber plants (cotton, flax, hemp, jute); citrus fruit (orange, lemon, grape, citrus); vegetables (spinach, lettuce, asparagus, cabbage, carrot, onion, tomato, potato, red pepper); lauraceae (avocado, cinnamon, camphor) or plants such as tobacco, nuts, coffee, eggplant, sugar cane, tea, pepper, grapes, bananas and natural rubber plants, and ornamental plants.

Crop plants to which the fungicidal compositions of the present invention are particularly suitable include rice, cucumber, melon, cabbage, grape, red pepper, green pepper, watermelon, pumpkin, tobacco, citrus, apple, tomato, banana, corn, asparagus, lettuce, calla, cauliflower, cabbage, konjac, oilseed rape, celery, tobacco, onion, green stem vegetable, tomato, eggplant, carrot, green onion, chinese cabbage, potato, lettuce, oilseed rape.

The bactericidal composition is particularly suitable for preventing and treating rice sheath blight disease, rice blast, false smut, corn big spot, corn small spot, corn rust, wheat scab, soybean rust, wheat rust, apple alternaria leaf spot, tomato early blight, tomato late blight, peanut rust and pear scab.

The use of the fungicidal compositions according to the invention for the protection of plants, plant propagation material and plant organs that grow at a later time.

The application of the bactericidal composition to the site needing control to control pathogenic or saprophytic harmful fungi in soil or culture medium.

The use of the fungicidal composition according to the invention for the treatment of seeds to protect the seeds from infestation by phytopathogens carried.

A method for controlling or preventing infestation of cultivated plants by phytopathogenic fungi, which method comprises applying the fungicidal composition of the invention to the phytopathogenic fungi and/or their environment or to the plants, to plant propagation material and to plant organs, to the soil or to cultivation media, to materials or to spaces which grow subsequently.

A method of controlling or preventing infestation of cultivated plants by phytopathogenic fungi which comprises applying the fungicidal composition of the present invention to foliage of the plants.

A method of controlling or preventing infestation of cultivated plants by phytopathogenic fungi which comprises applying a fungicidal composition of the present invention to plant propagation material and to plant organs which grow thereafter.

A method of controlling or preventing infestation of cultivated plants by phytopathogenic fungi, which method comprises applying a fungicidal composition according to the invention to the soil or to a cultivation medium.

A method of controlling or preventing infestation of a cultivated plant by phytopathogenic fungi, which method comprises applying said fungicidal composition to the phytopathogenic fungi of the plant and/or to its environment, or to the plant, to plant propagation material and to plant organs, soil or cultivation media, materials or spaces which grow at a time before or after the cultivated plant is infested.

A method for controlling or preventing infestation of cultivated plants by phytopathogenic fungi, which comprises applying the fungicidal compositions of the invention in seed treatment, foliar application, stem application, drenching, instillation, pouring, spraying, dusting, scattering or smoking, or the like, to the phytopathogenic fungi and/or to the environment thereof, or to the plants, to plant propagation material and to the organs, the soil or to cultivation media, materials or spaces to be grown subsequently, at an agronomically effective and substantially non-phytotoxic application rate.

The bactericidal composition can be used as a foliar bactericide in crop protection, and also can be used as a bactericide for seed dressing and as a soil bactericide.

The bactericidal composition of the present invention can treat all plants. "plant" means all plants and plant populations such as desirable and undesirable wild plants, cultivars, and plant varieties (whether or not protected by the plant variety or plant cultivar rights). Cultivated plants and plant varieties may be plants obtained by conventional propagation and cultivation methods, which may be supplemented or supplemented by one or more biotechnological methods, for example using dihaploids, protoplast fusion, random and directed mutations, molecular or genetic markers, or using bioengineering and genetic engineering methods. Plant parts are understood to mean all parts and organs of plants above and below the ground, such as shoots, leaves, flowers and roots, such as leaves, needles, stems, branches, flowers, fruit bodies, fruits and seeds, and also roots, bulbs and rhizomes. Also plants and vegetative and generative propagation material, for example cuttings, bulbs, rhizomes, runners and seeds, belong to the plant part.

The term "plant propagation material" is understood to mean all reproductively competent plant parts, such as seeds, which can be used for the propagation of the latter, and also vegetative materials, such as cuttings or tubers (e.g. potatoes). Thus, plant parts as used herein include plant propagation material. Mention may be made, for example, of seeds, roots, fruits, tubers, bulbs, rhizomes and plant parts. Germinated plants and useful plants to be inhibited after germination or after emergence from the soil. The young plants can be protected prior to transplantation by a total or partial treatment by dipping.

The fungicidal compositions according to the invention are suitable for protecting the seed of any plant variety applied in agriculture, in greenhouses, in forestry or in horticulture-or grape cultivars. In particular, it takes the form of seeds of cereals (such as wheat, barley, rye, triticale, millet, oats), maize, cotton, soybeans, rice, potatoes, sunflowers, beans, coffee, sugar beets, peanuts, oilseed rape, olives, cocoa, sugar cane, tobacco, vegetables (such as tomatoes, cucumbers, onions and lettuce), turfgrass and ornamental plants. The treatment of cereal and vegetable seeds is of vital importance.

The control of phytopathogenic fungi which damage the post-emergent plants is effected primarily by treating the soil and the aerial parts of the plants with crop protection agents.

The fungicidal compositions of the present invention may also be used to prevent or control a variety of pathogenic or saprophytic fungi and bacteria in soils or cultivation media. Examples of methods for applying a chemical to soil include a method in which a liquid chemical is applied directly to the roots of a plant or a seedling bed for raising seedlings, with or without dilution, a method in which granules are spread to the roots of a plant or a seedling bed for raising seedlings, a method in which powder, water dispersible granules, or the like are sprayed to soil and mixed with the entire soil before sowing, and a method in which powder, water dispersible granules, or the like are sprayed to planting holes or planting furrows after dilution before sowing or before planting a plant, followed by sowing.

The germicidal compositions of the present invention may also be used to protect stored materials from fungal and bacterial infestation.

According to the invention, the term "stock" is understood to mean natural substances and processed forms thereof of plant or animal origin which have been derived from the natural life cycle and are intended to be preserved for a long period of time. Storage products of plant origin, for example plants or parts thereof, such as stems, leaves, tubers, seeds, fruits or grains, can be protected in the freshly harvested state or in processed form, such as (pre) drying, wetting, comminuting, grinding, pressing or baking. Or wood, coarse wood forms such as construction timber, utility poles and fences; or in finished form, such as furniture or articles made of wood. The animal-derived stock is hide, leather, hair, etc. The compositions according to the invention can prevent fungal or bacterial attack such as corrosion, discoloration or mildew on storage. "stock" is preferably understood to mean natural substances of plant origin and processed forms thereof, more preferably fruits or vegetables, processed forms thereof.

Therefore, the bactericidal composition can be used as a foliar fungicide in crop protection, can be used as a fungicide for seed dressing and as a soil fungicide, and can be used as a preservative for postharvest storage.

The germicidal compositions of the present invention may be applied by various treatment methods, such as:

-spraying a liquid comprising the fungicidal composition onto the aerial parts of the plants;

-dusting, incorporating granules or powders in the soil, spraying around said plants and, in the case of tree injection or painting;

-coating or film coating the seeds of the plants;

when the preservative is used for preserving and refreshing picked fruits or vegetables, the liquid is diluted by 200 to 2000 times by using water, and the fruits are drained after being soaked.

The present invention provides a method of controlling plant pathogenic bacteria, either therapeutically, prophylactically or eradicated.

The fungicidal composition of the present invention can be used in the form of a conventional chemical preparation, for example, emulsifiable concentrate, wettable powder, suspension, liquid, granule, seed coating, and the like, and the application amount thereof varies depending on the mixing ratio of the active ingredients, weather conditions, the chemical preparation, the application time, the application method, the application site, the control of target pests, the target crop, and the like.

Typically for leaf treatment: 0.1 to 10000g/ha, preferably 10 to 1000g/ha, more preferably 50 to 500g/ha; for dipping or instillation administration, the dosage may even be reduced, particularly when an inert substrate such as asbestos or perlite is applied;

-for seed treatment: 2-5000g/100kg of seeds, preferably 3-1000g/100kg of seeds;

-applying a treatment to the soil or water surface: 0.1 to 10000g/ha, preferably 1 to 1000g/ha.

For the postharvest preservation of fruits and vegetables, the solution can be diluted by 200-2000 times, and the fruits are drained after fruit soaking.

The above-mentioned dosages are only typical exemplary dosages, and the person skilled in the art will adjust the application rate in the actual application according to the actual circumstances and needs, in particular according to the nature of the plants or crops to be treated and the severity of the germs.

The bactericidal composition can be mainly prepared in the form of preparations, namely, all the substances in the composition are mixed; the ingredients of the composition may also be provided in a single dose, mixed in a tub or tank prior to use, and then diluted to the desired concentration. The preparation form provided by the invention is preferably the main form.

The sterilization composition containing epoxiconazole and isoprothiolane can prevent and treat fungal and bacterial diseases of various vegetables, fruits, fruit trees and cereal crops. The composition containing epoxiconazole and isoprothiolane is very effective not only against common plant pathogens but also against pathogens which have developed resistance to drugs, and has excellent control effects even under conditions where diseases continue to develop.

The bactericidal composition containing epoxiconazole and isoprothiolane shows unexpected synergistic effect when active compounds are present in the bactericidal composition of the invention in a specific weight ratio.

The fungicidal compositions of the invention can also be used in combination with other agents having herbicidal, insecticidal or fungicidal properties, in particular with protective fungicides, and also with insecticides, protectants, growth regulators, plant nutrients or soil conditioners.

Compared with the prior art, the invention has at least the following beneficial effects:

the epoxiconazole and the isoprothiolane which are active ingredients in the bactericidal composition have a synergistic effect, and can be used for preventing and treating rice sheath blight, rice blast, false smut of rice, big leaf spot of corn, small leaf spot of corn, rust disease of corn, scab of wheat, soybean rust disease, wheat rust disease, alternaria leaf spot of apple, early blight of tomato, late blight of tomato, peanut rust disease or pear scab.

Detailed Description

For the purpose of facilitating an understanding of the present invention, the present invention will now be described by way of examples. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.

Formulation examples:

example 1 wettable powder of 5% epoxiconazole and 30% isoprothiolane

Active compounds, various auxiliary agents, fillers and the like are mixed according to the proportion of the formula, and the mixture is crushed by a superfine crusher to obtain the wettable powder of 5 percent epoxiconazole and 30 percent isoprothiolane.

Example 2 Epoxiconazole 5% + isoprothiolane 30% emulsifiable concentrate

Mixing the active compound, various auxiliary agents, solvents and the like according to the proportion of the formula, and uniformly stirring to obtain a uniform and transparent phase, thereby obtaining the emulsifiable solution of 5 percent epoxiconazole and 30 percent isoprothiolane.

Example 3 Epoxiconazole + isoprothiolane 40% emulsifiable concentrate

Mixing the active compound, various auxiliary agents, solvents and the like according to the proportion of the formula, and uniformly stirring to obtain a uniform and transparent phase, thereby obtaining the emulsifiable solution of 8 percent epoxiconazole and 40 percent isoprothiolane.

Example 4 wettable powder of 2% epoxiconazole +18% isoprothiolane

The components are mixed according to a proportion, ground and crushed to prepare wettable powder of 2 percent epoxiconazole and 18 percent isoprothiolane.

Example 5% Epoxiconazole +35% isoprothiolane Water dispersible granules

Epoxiconazole, isoprothiolane, a dispersant, a wetting agent, a disintegrating agent and a filler are uniformly mixed according to the proportion of a formula, and are crushed into wettable powder through airflow; and adding a certain amount of water, mixing, extruding and forming materials, and drying and screening to obtain the water dispersible granule containing 5% epoxiconazole and 35% isoprothiolane.

Example 6 Epoxiconazole +30% isoprothiolane Suspoemulsion 5%

Oil phase:

30 percent of isoprothiolane

Oleic acid methyl ester 20%

Ethoxylated castor oil 5%

Water phase:

5 percent of epoxiconazole

Sodium salt of sulfonated naphthalene sulfonic acid-formaldehyde condensation product 1%

The water is complemented to 100 percent

Dissolving isoprothiolane in methyl oleate, and adding ethoxylated castor oil to obtain an oil phase; grinding and/or shearing epoxiconazole, sodium salt of a sulfonated naphthalene sulfonic acid-formaldehyde condensation product and water at a high speed according to a formula to obtain an epoxiconazole suspending agent; the oil phase was added to the aqueous phase under stirring to give a suspoemulsion of 5% epoxiconazole +30% isoprothiolane.

Example 7 wettable powder of epoxiconazole 15% and isoprothiolane 75%

The components are mixed according to a proportion, ground and crushed to prepare wettable powder of 15 percent epoxiconazole and 75 percent isoprothiolane.

Example 8 coated granules of 2% epoxiconazole +18% isoprothiolane

The finely ground fungicidal active compound is homogeneously spread on the carrier moistened with polyethylene glycol in a mixer. In this way dust-free coated granules of 2% epoxiconazole +18% isoprothiolane are obtained.

Example 9 Epoxiconazole +30% isoprothiolane granules 5%

The active compound is mixed with the adjuvant and ground, moistened with water, granulated and then dried in an air stream to give granules of 5% epoxiconazole +30% isoprothiolane.

Example 10% Epoxiconazole +25% isoprothiolane seed coating agent

The seed coating agent of 5 percent epoxiconazole and 25 percent isoprothiolane is obtained by mixing the components in proportion and grinding and/or shearing at high speed.

Example 11 suspension of 5% epoxiconazole +35% isoprothiolane

Dissolving isoprothiolane in SOLVESSO ^TM 200, adding ethoxylated castor oil to obtain the oil phase of isoprothiolane.

Mixing the fatty alcohol-polyoxyethylene ether sulfosuccinic acid monoester disodium, the modified calcium lignosulfonate, the epoxiconazole and the water in proportion, and grinding and/or shearing at a high speed to obtain the epoxiconazole suspending agent.

Adding the oil phase containing isoprothiolane into the water suspending agent containing epoxiconazole to obtain a suspending agent of 5% epoxiconazole and 35% isoprothiolane.

Example 12 Epoxiconazole +36% isoprothiolane emulsifiable concentrate

The components are prepared according to the proportion, and are uniformly stirred to obtain a uniform phase, so that the missible oil containing 6 percent of epoxiconazole and 36 percent of isoprothiolane is obtained.

Example 13 Epoxiconazole +50% isoprothiolane Water dispersible granules 5%

Epoxiconazole, isoprothiolane, a dispersant, a wetting agent, a disintegrating agent and a filler are uniformly mixed according to the proportion of a formula, and are crushed into wettable powder through airflow; adding a certain amount of water, mixing and extruding to obtain the finished product. And drying and screening to obtain the water dispersible granule of 5 percent epoxiconazole and 50 percent isoprothiolane.

Example 14% Epoxiconazole +30% isoprothiolane suspension

The active components, the dispersant, the wetting agent, the water and the like are uniformly mixed according to the proportion of the formula, and the suspending agent of 5 percent epoxiconazole and 30 percent isoprothiolane is obtained by grinding and/or high-speed shearing and controlling the particle size to be below 2 mu m.

Example 15 aqueous emulsion of 5% Epoxiconazole +30% isoprothiolane

Oil phase:

water phase:

dissolving epoxiconazole and isoprothiolane in methyl oleate, and adding polystyrene to obtain an oil phase; uniformly mixing the components in the formula to obtain a water phase; adding the oil phase into the water phase under stirring to obtain the 5% epoxiconazole and 30% isoprothiolane aqueous emulsion.

Example 16% epoxiconazole +90% isoprothiolane

Epoxiconazole is 10%

90 percent of isoprothiolane

Comparative example 1 Epoxiconazole +12% isoprothiolane emulsifiable concentrate

/>

Mixing the active compound, various auxiliary agents, solvents and the like according to the proportion of the formula, and uniformly stirring to obtain a uniform and transparent phase, thereby obtaining the emulsifiable solution of 6 percent epoxiconazole and 12 percent isoprothiolane.

Comparative example 2 Epoxiconazole 12% and isoprothiolane 12% emulsifiable concentrate

Mixing the active compound, various additives, solvents and the like according to the proportion of the formula, and uniformly stirring to obtain a uniform and transparent phase, thereby obtaining the emulsifiable concentrate of 12 percent epoxiconazole and 12 percent isoprothiolane.

Comparative example 3% epoxiconazole +12% isoprothiolane emulsifiable concentrate

Mixing the active compound, various auxiliary agents, solvents and the like according to the proportion of the formula, and uniformly stirring to obtain a uniform and transparent phase, thereby obtaining the emulsifiable solution of 3 percent epoxiconazole and 12 percent isoprothiolane.

The proportions in the above examples and comparative examples are in weight percent.

Biological test example

Test of drug efficacy

Experimental example 1: test for preventing rice blast

Leaves of potted rice seedlings were sprayed with an aqueous dilution having the active compound concentration specified below. The next day, the treated rice seedlings were inoculated with a spore suspension of Pyricularia oryzae. The pots were then placed in a chamber with atmospheric humidity (90% -95%) at 20-22 ℃ for 24 hours. During this time, the spores germinate and the germ tubes infiltrate into the leaf tissue. The next day, the test plants were returned to the greenhouse and incubated at 20-22 ℃ and 65% -70% relative atmospheric humidity for an additional 7 days. The degree of rice blast development on the leaves was then visually observed.

TABLE 1 control Effect on Rice blast

As can be seen from the data in Table 1, when the composition of epoxiconazole and isoprothiolane is used for controlling rice blast, the epoxiconazole serving as an active ingredient accounts for 2% -15% of the bactericidal composition, and the isoprothiolane accounts for 18% -75% of the bactericidal composition, better control effect is obtained.

Experimental example 2: test for preventing rice false smut

Rice plants at the end of the rice panicle were sprayed with an aqueous dilution having the active compound concentration specified below. The following day the treated rice plants were inoculated with a suspension of rice false smut spores. The pots were then placed in a chamber with atmospheric humidity (90% -95%) at 20-22 ℃ for 24 hours. The next day, the test plants are returned to the greenhouse and incubated at 20-22 ℃ and 65% -70% relative atmospheric humidity for an additional 45 days. Then, the degree of development of false smut of rice was visually observed.

TABLE 2 prevention and treatment effects on false smut of rice

As can be seen from the data in Table 2, when the composition of epoxiconazole and isoprothiolane is used for preventing and treating false smut of rice, the epoxiconazole serving as an active ingredient accounts for 2% -15% of the bactericidal composition, and the isoprothiolane accounting for 18% -75% of the bactericidal composition, better prevention and treatment effects can be obtained.

Experimental example 3: test for preventing and controlling rice sheath blight disease

Leaves of potted rice seedlings were sprayed with an aqueous dilution having the active compound concentration specified below. The next day, the treated rice seedlings were inoculated with a spore suspension of rice sheath blight disease. The pots were then placed in a chamber with atmospheric humidity (90% -95%) at 20-22 ℃ for 24 hours. During this time, the spores germinate and the germ tubes infiltrate into the leaf tissue. The following day, the test plants were returned to the greenhouse and incubated at 20-22 ℃ and 65% -70% relative atmospheric humidity for an additional 7 days. The degree of rice sheath blight development on the leaves was then visually assessed.

TABLE 3 preventive and therapeutic effects on sheath blight of rice

As can be seen from the data in Table 3, when the composition of epoxiconazole and isoprothiolane is used for controlling rice sheath blight disease, the epoxiconazole serving as an active ingredient accounts for 2% -15% of the bactericidal composition, and the isoprothiolane accounts for 18% -75% of the bactericidal composition, better control effect is obtained.

Experimental example 4: prevention and effect test for northern leaf blight of corn

Leaves of potted maize plants are sprayed with an aqueous dilution having the active compound concentration specified below. The following day, the treated maize plants were inoculated with a spore suspension of northern leaf blight. The pots were then placed in a chamber with atmospheric humidity (90% -95%) at 20-22 ℃ for 24 hours. During this time, the spores germinate and the germ tubes infiltrate into the leaf tissue. The next day, the test plants were returned to the greenhouse and incubated at 20-22 ℃ and 65% -70% relative atmospheric humidity for an additional 7 days. The leaves were then visually inspected for the extent of development of northern leaf blight.

TABLE 4 controlling effect on northern leaf blight of corn

As can be seen from the data in Table 4, when the composition of epoxiconazole and isoprothiolane is used for controlling the northern leaf blight of corn, the epoxiconazole serving as an active ingredient accounts for 2% -15% of the bactericidal composition, and the isoprothiolane accounts for 18% -75% of the bactericidal composition, better control effect is obtained.

Experimental example 5: test for preventing corn rust

Leaves of potted maize plants were sprayed with an aqueous dilution having the active compound concentration specified below. The following day the treated maize plants were inoculated with a spore suspension of maize rust. The pots were then placed in a chamber with atmospheric humidity (90% -95%) at 20-22 ℃ for 24 hours. During this time, the spores germinate and the germ tubes infiltrate into the leaf tissue. The next day, the test plants were returned to the greenhouse and incubated at 20-22 ℃ and 65% -70% relative atmospheric humidity for an additional 7 days. The leaves were then visually inspected for the extent of development of corn rust.

TABLE 5 control Effect on corn rust

As can be seen from the data in Table 5, when the composition of epoxiconazole and isoprothiolane is used for controlling the corn rust, the epoxiconazole serving as an active ingredient accounts for 2% -15% of the bactericidal composition, and the isoprothiolane accounts for 18% -75% of the bactericidal composition, better control effect is obtained.

The following two points can also be seen from the data in tables 1-5 above:

as can be seen from comparison of examples 1 to 15 with comparative examples 1 to 3, examples 1 to 15 can achieve more excellent control effects by controlling the ratio of epoxiconazole to isoprothiolane within the range of 1 to 1; compared with a single epoxiconazole emulsifiable solution or a single isoprothiolane emulsifiable solution, the composition can obtain better control effect, so that the invention can further show that the synergistic effect can be exerted by controlling the proportion of the epoxiconazole to the isoprothiolane within the range of 1-5-1.

When the mass ratio of the epoxiconazole to the isoprothiolane is controlled to be 1; the control effect is best when the formulation is missible oil, and therefore, the best control effect can be obtained when the mass ratio of the epoxiconazole to the isoprothiolane is controlled to be 1.

Claims (5)

1. The composition containing epoxiconazole and isoprothiolane is characterized in that active ingredients comprise epoxiconazole and isoprothiolane, the weight ratio of the epoxiconazole to the isoprothiolane is 1 to 6, the mass of the epoxiconazole accounts for 5 to 15 percent of the composition, the mass of the isoprothiolane accounts for 20 to 50 percent of the composition, and the dosage form of the composition is missible oil.

2. Use of a composition according to claim 1 for controlling fungi on cereals, vegetables, fruits, ornamentals or grapevines.

3. Use of the composition according to claim 1 for controlling rice sheath blight disease, rice blast, rice false smut, corn northern leaf blight, corn rust.

4. A method of controlling phytopathogenic fungi, comprising the action of a composition according to claim 1 on the pathogenic fungi and/or their environment or on plants, seeds, soils, materials or spaces, storage.

5. A method for controlling phytopathogenic fungi, characterized in that a composition according to claim 1 is applied to the phytopathogenic fungi and/or their environment, or to the plants, plant propagation material and plant organs, soil or cultivation media, materials or spaces which grow at a later time, by seed treatment, foliar application, stem application, drenching, dripping, pouring, spraying, dusting, scattering or fuming.