CN113759142B - Blood sampling method and blood sampling device - Google Patents
Blood sampling method and blood sampling device Download PDFInfo
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- CN113759142B CN113759142B CN202010501406.3A CN202010501406A CN113759142B CN 113759142 B CN113759142 B CN 113759142B CN 202010501406 A CN202010501406 A CN 202010501406A CN 113759142 B CN113759142 B CN 113759142B
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- 238000000034 method Methods 0.000 title claims abstract description 31
- 238000010241 blood sampling Methods 0.000 title claims description 43
- 238000012360 testing method Methods 0.000 claims abstract description 171
- 238000000926 separation method Methods 0.000 claims abstract description 104
- 210000002966 serum Anatomy 0.000 claims abstract description 69
- 230000000149 penetrating effect Effects 0.000 claims abstract 3
- 238000004140 cleaning Methods 0.000 claims description 18
- 238000002955 isolation Methods 0.000 claims description 14
- 238000005070 sampling Methods 0.000 claims description 14
- 238000012546 transfer Methods 0.000 claims description 7
- 238000007664 blowing Methods 0.000 claims description 6
- 230000000087 stabilizing effect Effects 0.000 claims description 3
- 238000013022 venting Methods 0.000 claims description 2
- 210000004369 blood Anatomy 0.000 abstract description 31
- 239000008280 blood Substances 0.000 abstract description 31
- 239000007788 liquid Substances 0.000 description 5
- 238000011109 contamination Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 230000035515 penetration Effects 0.000 description 4
- 230000000712 assembly Effects 0.000 description 3
- 238000000429 assembly Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000011166 aliquoting Methods 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000004891 communication Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
Abstract
The blood sample separation method and the blood sample separation device comprise a sample separation needle, a puncture needle and a driving mechanism for connecting the sample separation needle and the puncture needle, wherein the puncture needle is provided with a hollow structure, and the sample separation needle is arranged in the hollow structure in a penetrating way; the method comprises the following steps: the driving mechanism drives the puncture needle to penetrate through the test tube cover of the target test tube and drives the sample separating needle to move downwards in the hollow structure of the puncture needle; the sample separating needle sucks serum in the target test tube; the drive mechanism drives the needle up in the hollow structure of the spike and drives the spike up to clear the tube cap of the target tube. The test tube cover penetrating the test tube is pierced through the puncture needle, then the sample separation needle moves downwards in the hollow structure of the puncture needle to suck serum in the test tube, so that the blood sample can be sucked without taking the test tube cover, the test tube cover is covered on the tube body of the test tube after sucking the blood sample, the cover or the film is not required, the biosafety risk can be effectively avoided, the workload is reduced, and the cost is saved.
Description
Technical Field
The application relates to the technical field of medical instruments, in particular to a blood sampling method and blood sampling equipment.
Background
For people of different types and different conditions, when the hospital takes blood, different strategies are adopted, such as infants, the elderly with weak constitution or critical patients, the hospital draws less blood to the people, the great negative influence of excessive blood drawing on the bodies of the people is avoided, the number of test items for patients is not small, and the policy adopted by hospitals is to sample their blood sampling samples to support parallel testing of different service items, instead of consuming one and more blood sampling samples for each service item, so that sample sampling is more common in hospitals.
The sample divides the appearance again and divide into artifical branch appearance and instrument automatic branch appearance, and artifical branch appearance inefficiency, branch appearance result are inaccurate, to high pathogenicity sample, and the operation mr still has the risk of being infected, to instrument automatic branch appearance, divide appearance efficiency higher, branch appearance result is accurate, because the operation mr does not carry out direct operation, at the risk greatly reduced that divides appearance link to be infected. However, for biochemical immunization business, the precondition for sample separation is that the sample needs to be uncapped so that the sample separation instrument can draw serum from the test tube. Therefore, at present, the sample needs to be removed in advance, the sample is volatilized to the air in the process of removing the cover and after removing the cover, and the sample is covered/coated later so as to prevent volatilization and dilution storage or prevent biological pollution caused by direct discarding, so that the working flow is increased, the workload is increased, the cost is increased, and the biological safety risk is increased.
Disclosure of Invention
Based on the above, the application provides a blood sample separation method and blood sample separation equipment, which aim at solving the technical problems that the existing sample separation needs to be uncapped and the like.
In a first aspect, an embodiment of the present application provides a blood sampling method, for a blood sampling device, where the blood sampling device includes a sampling needle, a puncture needle, and a driving mechanism that connects the sampling needle and the puncture needle, where the puncture needle is provided with a hollow structure, and the sampling needle is penetratingly arranged in the hollow structure;
the method comprises the following steps:
the driving mechanism drives the puncture needle to penetrate through a test tube cover of a target test tube and drives the sample separation needle to move downwards in a hollow structure of the puncture needle;
the sample separation needle sucks serum in the target test tube;
the drive mechanism drives the sample separation needle to move upwards in the hollow structure of the puncture needle and drives the puncture needle to move upwards to leave the test tube cover of the target test tube.
In a second aspect, embodiments of the present application provide a blood sampling device comprising:
the puncture needle is provided with a hollow structure;
the sample separating needle penetrates through the hollow structure and sucks serum in the target test tube;
the driving mechanism is connected with the sample dividing needle and the puncture needle, and drives the puncture needle to penetrate through the test tube cover of the target test tube, drives the sample dividing needle to move downwards in the hollow structure of the puncture needle, drives the sample dividing needle to move upwards in the hollow structure of the puncture needle, and drives the puncture needle to move upwards to leave the test tube cover of the target test tube.
The embodiment of the application provides a blood sample separating method and blood sample separating equipment, passes the test tube lid of target test tube through the pjncture needle, then the sample separating needle is in the hollow structure of pjncture needle down move in order to draw the serum in the target test tube to need not to take off the test tube lid and just can draw the blood sample, in addition after drawing the blood sample the test tube lid still cover on the body of target test tube, need not to add lid or adding the membrane, can effectively avoid biosafety risk, reduce work load, save the cost.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the disclosure of embodiments of the present application.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings that are needed in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present application, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic flow chart of a blood sampling method according to an embodiment of the present disclosure;
FIG. 2 is a schematic view of a blood sampling device according to an embodiment of the present disclosure;
FIG. 3 is a schematic view of the lancet and the needle above the target tube;
FIG. 4 is a schematic view of the lancet beginning to pass through the test tube cap;
FIG. 5 is a schematic view of the penetration needle through the test tube cap;
FIG. 6 is a schematic view of the needle moving downward in the needle;
FIG. 7 is a schematic view of the lower end of the needle extending into the serum;
FIG. 8 is a schematic view of the sample separation needle and the lancet exiting the test tube cap;
fig. 9 is a schematic structural view of a blood sampling device according to another embodiment of the present application.
Reference numerals: 100. a blood sampling device; 110. a sample separating needle; 120. a puncture needle; 121. a hollow structure; 122. an isolation structure; 123. an electromagnetic valve; 130. a driving mechanism; 140. an air blowing mechanism;
10. a target test tube; 11. a test tube cover; 21. a serum layer; 201. the surface of serum; 22. blood cell layers.
Detailed Description
The following description of the embodiments of the present application will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are some, but not all, of the embodiments of the present application. All other embodiments, which can be made by one of ordinary skill in the art without undue burden from the present disclosure, are within the scope of the present disclosure.
The flow diagrams depicted in the figures are merely illustrative and not necessarily all of the elements and operations/steps are included or performed in the order described. For example, some operations/steps may be further divided, combined, or partially combined, so that the order of actual execution may be changed according to actual situations.
Some embodiments of the present application are described in detail below with reference to the accompanying drawings. The following embodiments and features of the embodiments may be combined with each other without conflict.
Referring to fig. 1, fig. 1 is a schematic flow chart of a blood sampling method according to an embodiment of the present application. The blood sampling method can be applied to blood sampling equipment for sucking serum from a test tube and the like.
Referring to fig. 2, fig. 2 is a schematic structural diagram of a blood sampling device 100 according to an embodiment of the present application.
As shown in fig. 2, the blood sampling device 100 includes a sampling needle 110, a puncture needle 120, and a driving mechanism 130 connecting the sampling needle 110 and the puncture needle 120.
In some embodiments, the sample separation needle 110 comprises a needle body and a needle head which are arranged along the direction of a length axis, wherein a fluid passage which is communicated with each other is arranged in the needle body and the needle head, the fluid passage extends along the direction of the length axis, and one end opening of the fluid passage is arranged on the needle head.
The needle 110 is used to aspirate serum from the target tube 10. Upon the creation of a negative pressure in the fluid path, the needle 110 is able to draw a blood sample, such as serum, from the opening.
Specifically, as shown in fig. 2 to 7, the puncture needle 120 is provided with a hollow structure 121, and the sample separation needle 110 is inserted into the hollow structure 121. For example, the needle 110 can move within the hollow structure 121 relative to the needle 120.
In some embodiments, drive mechanism 130 includes a first drive assembly for driving movement of sample needle 110 and a second drive assembly for driving movement of spike 120; it will be appreciated that the drive mechanism 130 may independently drive the needle 110, may independently drive the needle 120, and that the drive mechanism 130 may also illustratively drive the needle 110 and the needle 120 to move simultaneously.
The first and second driving assemblies may drive the sample separation needle 110 and the puncture needle 120 to move through electromagnetic driving, or the first and second driving assemblies may drive the sample separation needle 110 and the puncture needle 120 to move through pneumatic driving, or the first and second driving assemblies may drive the sample separation needle 110 and the puncture needle 120 to move through motor rotation.
As shown in fig. 1, the blood sampling method according to the embodiment of the present application includes steps S110 to S130.
Step S110, the driving mechanism 130 drives the puncture needle 120 through the tube cap 11 of the target tube 10, and drives the sample separation needle 110 to move downward in the hollow structure 121 of the puncture needle 120.
In some embodiments, the method further comprises: moving the target test tube 10 to a preset test tube position, and righting and stabilizing the test tube; the drive mechanism 130 drives the puncture needle 120 and the sample separation needle 110 to move in the horizontal direction above the target test tube 10.
In some embodiments, the blood sampling device 100 further comprises a tube transfer device for moving the tube to a preset tube position.
Illustratively, the tube transfer device carries the target tube 10, moves the target tube 10 to a preset tube position, and straightens and stabilizes.
Illustratively, the blood sampling apparatus 100 receives a test tube put in by a user, grips the test tube into the test tube transfer device, or the blood sampling apparatus 100 directly receives the test tube from other apparatus into the test tube transfer device to move the test tube to a preset test tube position by the test tube transfer device.
Illustratively, the blood sampling device 100 further includes a scanning device (not shown in the figure) disposed at a barcode scanning position, and the test tube transferring device may transfer the test tube to the barcode scanning position, and the scanning device recognizes a sample identifier, such as a number ID, of the test tube, so that the blood sampling device 100 may obtain information of a current test tube, for example, may determine that a blood sample in the test tube needs to be sampled to at most a few sub-test tubes according to the information of the current test tube, and may determine a sample separating volume of each sub-test tube.
Illustratively, the blood sample separation apparatus 100 further includes a tube centering mechanism disposed at a predetermined tube position, the tube centering mechanism being capable of centering the tube after the tube reaches the tube position so as to separate a blood sample in the tube.
Illustratively, the test tube currently in need of the sample separation operation is the target test tube 10.
In some embodiments, drive mechanism 130 also drives spike 120 and aliquoting needle 110 to move in a horizontal direction above target tube 10.
Illustratively, the blood sampling device 100 has test tubes at one or more test tube locations, and the blood sampling device 100 can determine information about the test tubes at each test tube location and determine the current target test tube 10 based on that information.
Illustratively, the drive mechanism 130 is capable of driving the lancet 120 or the needle 110 up or down at the test tube location.
As shown in FIG. 3, the lancet 120 and the needle 110 are positioned above the target cuvette 10.
As shown in fig. 3-5, the drive mechanism 130 drives the spike 120 through the tube cap 11 of the target tube 10.
The test tube cap 11 may be also referred to as a test tube cap, and may be made of rubber, for example, to prevent contamination of the blood sample or contamination of the blood sample by release of the blood sample.
In some embodiments, drive mechanism 130 drives spike 120 downward, proximate to tube cap 11, into abutment with tube cap 11 and through tube cap 11.
Illustratively, the test tube cap 11 is provided with an insertion portion through which the puncture needle 120 passes through the test tube cap 11. Illustratively, the thickness of the penetration is smaller than the other areas of the cuvette lid 11 to facilitate penetration. The penetration portion is, for example, a circular region.
Illustratively, the drive mechanism 130 may simultaneously drive the needle 110 downward as the needle 120 is driven through the tube cap 11, e.g., to maintain a relative rest between the needle 120 and the needle 110; of course, the drive mechanism 130 may not drive the needle 110 downward when driving the puncture needle 120 through the test tube cap 11.
In some embodiments, the drive mechanism 130 drives the needle 110 downward in the hollow structure 121 of the needle 120 as the drive mechanism 130 drives the needle 120 through the tube cap 11 of the target tube 10, or after the drive mechanism 130 drives the needle 120 through the tube cap 11 of the target tube 10.
Exemplary, fig. 5-7 are schematic diagrams of the drive mechanism 130 driving the sample separation needle 110 at various stages of downward movement in the hollow structure 121 of the piercing needle 120.
In some embodiments, as shown in fig. 3-7, the target tube 10 is a serum-loaded target tube 10. Illustratively, the blood contained in the target tube 10 is stratified into a blood cell layer 22 and a serum layer 21, and some or all of the serum is required to be drawn from the target tube 10.
Illustratively, the drive mechanism 130 drives the lancet 120 through the tube cap 11 of the target tube 10, including: the drive mechanism 130 drives the lancet 120 through the cuvette lid 11 of the target cuvette 10, and the lower end of the lancet 120 is above the surface 201 of the serum in the target cuvette 10.
As shown in fig. 5 to 7, the lower end of the puncture needle 120 is higher than the surface 201 of the serum in the target test tube 10, for example, the puncture needle 120 moves only a distance below the test tube cover 11 and is not in contact with the serum sample liquid, and thus the air pressure inside the target test tube 10 is vented to the atmosphere.
Illustratively, as shown in fig. 5-7, driving the sample separation needle 110 downward in the hollow structure 121 of the piercing needle 120 includes: the needle 110 is driven to move down in the hollow structure 121 of the needle 120 until the lower end of the needle 110 protrudes into the serum in the target test tube 10.
As shown in fig. 7, the lower end of the sample-dividing needle 110 extends into the serum in the target test tube 10.
For example, the blood sampling device 100 may receive the capacitance signal output from the sampling needle 110, convert the capacitance signal to output a corresponding voltage signal, amplify the voltage signal according to a set gain, output a gain voltage signal, and compare the gain voltage signal with a preset voltage threshold to determine whether the sampling needle 110 contacts the surface 201 of serum in the target test tube 10. After the needle 110 contacts the surface 201 of the serum in the target tube 10, the needle 110 is driven to continue to move downward by a predetermined distance so that the lower end of the needle 110 protrudes into the serum in the target tube 10. For the liquid level detection method, it will be appreciated by those skilled in the art that other methods of detecting the surface 201 of the serum, such as detecting the surface 201 of the serum using a change in resistance or inductance, may be used in addition to detecting the surface 201 of the serum using a change in capacitance. Correspondingly, a sample separation needle 110 adapted to the detection method of the surface 201 of the serum should be used. According to the electrical property change of the sample separation needle 110, a liquid level detection signal changing with the electrical property change of the sample separation needle 110 can be determined to the processor.
Illustratively, driving the needle 110 downward in the hollow structure 121 of the needle 120 until the lower end of the needle 110 protrudes into the serum in the target test tube 10 includes: detecting whether the lower end of the sample separating needle 110 touches the surface 201 of the serum in the target test tube 10 while driving the sample separating needle 110 to move downward; after the lower end of the sample separation needle 110 touches the surface, the sample separation needle 110 is driven to move downwards continuously for a first preset distance, and the first preset distance is determined according to a preset serum sucking amount.
For example, the blood sampling device 100 may determine the amount of blood currently drawn from the target test tube 10, that is, the amount of blood drawn from the target test tube 10 according to the information of the target test tube 10, determine a first preset distance for the lower end of the sampling needle 110 to move downward after touching the surface according to the amount of blood drawn, and control the driving mechanism 130 to drive the sampling needle 110 to move downward for the first preset distance.
Step S120, the sample separation needle 110 sucks the serum in the target test tube 10.
In some embodiments, after the lower end of the needle 110 extends into the serum in the target tube 10, the needle 110 aspirates the serum in the target tube 10.
Illustratively, when the needle 110 continues to move downward a first preset distance after the lower end touches the surface while moving downward, the needle 110 may draw a corresponding amount of serum, and quantitative drawing of the blood sample may be achieved.
In some embodiments, the sample separation needle 110 is driven to move downwards in the hollow structure 121 of the puncture needle 120 until the lower end of the sample separation needle 110 touches the surface 201 of the serum in the target test tube 10, and the sample separation needle 110 sucks the serum in the target test tube 10, so that the blood sample can be sucked more quickly. Illustratively, the blood sampling device 100 may detect the amount of serum drawn by the sampling needle 110, enabling quantitative drawing of a blood sample.
Step S130, the driving mechanism 130 drives the sample separation needle 110 to move upward in the hollow structure 121 of the puncture needle 120, and drives the puncture needle 120 to move upward to leave the cuvette lid 11 of the target cuvette 10.
In some embodiments, as shown in fig. 8, after the sample separation needle 110 draws a fixed amount of serum, the drive mechanism 130 first drives the sample separation needle 110 upward in the hollow structure 121 of the puncture needle 120, and then drives the puncture needle 120 upward to move away from the cuvette lid 11 of the target cuvette 10.
Illustratively, as shown in FIG. 8, the drive mechanism 130 may drive the needle 110 and the needle 120 to move upward simultaneously after driving the needle 110 to move upward a distance in the hollow structure 121 of the needle 120.
Illustratively, after the drive mechanism 130 drives the needle 110 up to a predetermined height, the needle 120 is driven up to move away from the tube cap 11 of the target tube 10. After the puncture needle 120 leaves the tube cap 11 of the target tube 10, the tube cap 11 also covers the tube body of the target tube 10.
In some embodiments, the separation needle 110 draws in isolation air when the separation needle 110 is driven to move upwards in the hollow structure 121 of the piercing needle 120 and when the distance between the lower end of the separation needle 110 and the surface 201 of the serum in the target test tube 10 reaches a second preset distance.
Illustratively, after the sample is sucked, the sample separating needle 110 is lifted, and the sample separating needle 110 is lifted a certain distance away from the liquid level in the lifting process to suck isolation air, so that the sample liquid can be prevented from being thrown out when the sample separating needle 110 moves upwards and/or horizontally, the outer wall of the needle tip can be ensured to have as few residual sample as possible, and the sample is prevented from being stained on the inner wall of the puncture needle 120.
In some embodiments, the needle 110 is vented quantitatively when the needle 110 is driven to move up the hollow structure 121 of the needle 120 and when the distance between the lower end of the needle 110 and the surface 201 of the serum in the target test tube 10 reaches a second predetermined distance.
Illustratively, during the lifting process of the sample separating needle 110, the inner wall of the sample separating needle 110 is subjected to quantitative exhaust, so that the residue of the outer wall of the sample separating needle 110 can be reduced, and meanwhile, the effective air section of the inner wall of the sample separating needle 110 is ensured to realize isolation.
In some embodiments, a space is provided between the outer wall of the needle 110 and the inner wall of the needle 120 that ensures that the air pressure inside the tube is vented to atmosphere.
In other embodiments, as shown in FIG. 9, a separation structure 122 is provided between the outer wall of the sample separation needle 110 and the inner wall of the piercing needle 120.
By way of example, the isolation structure 122 may include a piston, rubber pad, or the like. The isolation structure 122 enables the hollow structure 121 of the lancet 120 to form a closed space with respect to the atmosphere, so that the sample separation needle 110 can move up or down in the hollow structure 121 of the lancet 120 while ensuring that the hollow structure 121 of the lancet 120 can be sealed. Contamination released through the hollow structure 121 of the needle 120 can be reduced.
Illustratively, as shown in FIG. 9, the isolation mechanism or needle 120 has a solenoid valve 123 disposed on a sidewall thereof, the solenoid valve 123 being capable of venting or isolating the hollow structure 121 of the needle 120 from the atmosphere.
The method further comprises: when the driving mechanism 130 drives the puncture needle 120 to pass through the test tube cover 11 of the target test tube 10, the electromagnetic valve 123 is opened, so that the hollow structure 121 of the puncture needle 120 is communicated with the atmosphere, and the air pressure in the target test tube 10 is communicated with the atmosphere through the hollow structure 121, so that the suction back during the suction of the sample by the sample separation needle 110 is prevented, the cleaning water in the sample separation needle 110 is sucked back into the test tube, the sample is diluted, and the sample suction is inaccurate.
The method further comprises: the solenoid valve 123 is closed after the aliquoting needle 110 sucks the serum in the target test tube 10. For example, controlling the solenoid valve 123 to close to isolate the hollow structure 121 of the lancet 120 from the atmosphere after the sample is sucked by the sample separating needle 110, i.e., closing the communication between the inside of the test tube and the atmosphere, can reduce the contamination released through the hollow structure 121 of the lancet 120.
In some embodiments, as shown in fig. 9, the blood sampling device 100 further comprises: and an air blowing mechanism 140, wherein the air blowing mechanism 140 is connected with the puncture needle 120. Illustratively, the insufflation mechanism 140 comprises a syringe or air pump capable of providing positive pressure to the hollow structure 121 of the needle 120.
Illustratively, when the driving mechanism 130 drives the sample separation needle 110 to move upwards in the hollow structure 121 of the puncture needle 120, the air blowing mechanism 140 blows air to the outer wall of the sample separation needle 110, so as to blow the serum on the outer wall of the sample separation needle 110 back to the target test tube 10, reduce the residual of the serum on the outer wall of the sample separation needle 110, and can avoid the residual of the sample to the inner wall of the puncture needle 120. The sample separation needle 110 can quantitatively exhaust when the sample separation needle 110 moves upwards, so that the residue on the outer wall of the sample separation needle 110 can be reduced, and meanwhile, the effective air section on the inner wall of the sample separation needle 110 can be ensured to realize isolation. In addition, the sealed space formed by the inner wall of the puncture needle 120 can also perform better inner wall cleaning, and cross contamination between samples can be better avoided.
In some embodiments, the blood sampling apparatus 100 further includes a cleaning device (not shown) disposed at a predetermined cleaning position adjacent to the test tube position.
The method further comprises: the driving mechanism 130 drives the puncture needle 120 and the sample distributing needle 110 to move in the horizontal direction to the cleaning position, and the cleaning device cleans the puncture needle 120 and the sample distributing needle 110.
Illustratively, the drive mechanism 130 drives the lancet 120 and the needle 110 in a horizontal direction to a cleaning position. The cleaning device cleans the puncture needle 120 and the sample separation needle 110 when the drive mechanism 130 drives the puncture needle 120 and the sample separation needle 110 to move in the horizontal direction to the cleaning position.
Illustratively, cleaning the lancet 120 and the needle 110 includes: the inner and outer walls of the needle 120 are cleaned and the outer and inner walls of the needle 110 are cleaned.
Illustratively, the inner and outer walls of the needle 120 may be purged through a circular ring, the outer wall of the needle 110 may be purged through a circular ring, and the inner wall of the needle 110 may be purged using syringe suction. In some embodiments, the swab may also be moved axially relative to the needle 110 or 120 to clean the outer wall of the needle 110 or 120.
In some embodiments, the blood sampling device 100 can also be used to prepare satisfactory test tubes, for example, a user can randomly place test tubes with the test tube caps 11 into the blood sampling device 100, and the blood sampling device 100 can automatically meter test tubes. Illustratively, the blood sampling apparatus 100 is further capable of automatically printing a predetermined number of sub-tube bar codes according to the tube information of the identified main tube, adhering the sub-tube bar codes to the corresponding sub-tubes, and then righting the sub-tubes by the upright tilting mechanism and then transferring the sub-tubes to the sub-tube dispensing position. Of course, the test tube may also be manually placed at a corresponding location, such as at a sub-tube dispensing location.
Illustratively, after the sample separation needle 110 sucks serum in the target test tube 10, the driving mechanism 130 drives the sample separation needle 110 to move upward in the hollow structure 121 of the puncture needle 120 and drives the puncture needle 120 to move upward to leave the test tube cap 11 of the target test tube 10, and then the driving mechanism 130 drives the sample separation needle 110 and the puncture needle 120 to move in the horizontal direction to the sub-test tube dispensing position so that the sample separation needle 110 and the puncture needle 120 are located above the sub-test tubes.
Illustratively, the drive mechanism 130 may drive the needle 110 downward into the sub-tube, and the needle 110 may expel the aspirated serum into the sub-tube.
Illustratively, the sub-test tubes may not be provided with the test tube cover 11, and may be provided with the test tube cover 11. When the sub-tube is provided with the tube cap 11, the driving mechanism 130 drives the puncture needle 120 to pass through the tube cap 11 of the sub-tube and drives the sample separation needle 110 to move downward in the hollow structure 121 of the puncture needle 120, after which the sample separation needle 110 can discharge the sucked serum into the sub-tube, and after the completion of the discharge, the driving mechanism 130 drives the sample separation needle 110 to move upward in the hollow structure 121 of the puncture needle 120 and drives the puncture needle 120 to move upward to leave the tube cap 11 of the sub-tube.
For example, the suctioned serum sample may be dispensed into each sub-tube in a predetermined amount, and the number of sub-tubes may be one or may be plural, and may be specifically predetermined. Depending on the sample separation volume, the sample separation needle 110 may aspirate the sample from the main tube once, and possibly several times, to ensure that each sub-tube is separated to a sufficient serum sample.
According to the blood sample separation method and the blood sample separation device, the puncture needle can penetrate through the test tube cover of the target test tube, and then the sample separation needle moves downwards in the hollow structure of the puncture needle to suck serum in the target test tube, so that a blood sample can be sucked without taking the test tube cover, the test tube cover is covered on the tube body of the target test tube after sucking the blood sample, a cover or a film is not required, the biosafety risk can be effectively avoided, the workload is reduced, and the cost is saved. The problems that biological safety risks exist in the sample separation solutions provided by the current factories, the operation teacher is possibly infected, and the sample separation needs to be uncapped and then capped/filmed, so that the workload is increased and the cost is increased are solved.
In addition, the puncture needle can enable the air pressure in the test tube to be the same as the external atmospheric pressure, keep consistent, prevent the sample separation needle from sucking reversely when sucking samples, and enable the cleaning water in the sample separation needle to be sucked reversely into the test tube to dilute the samples, so that the samples are not sucked accurately; in addition, the puncture needle cover still plays the protection branch appearance needle in the outside of dividing the appearance needle, avoids dividing the askew problem of sample needle individual puncture insertion, and the puncture needle cover can avoid dividing the remaining sample of appearance needle outer wall to detain on the test tube lid in the branch appearance needle outside, prevents that the test tube lid outer wall from remaining the risk that has the sample to lead to biological pollution.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.
It should also be understood that the term "and/or" as used in this application and the appended claims refers to any and all possible combinations of one or more of the associated listed items, and includes such combinations.
The foregoing is merely a specific embodiment of the present application, but the protection scope of the present application is not limited thereto, and any equivalent modifications or substitutions will be apparent to those skilled in the art within the scope of the present application, and these modifications or substitutions should be covered in the protection scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.
Claims (13)
1. A blood sampling method, which is used for blood sampling equipment, and is characterized in that the blood sampling equipment comprises a sampling needle, a puncture needle and a driving mechanism for connecting the sampling needle and the puncture needle, wherein the puncture needle is provided with a hollow structure, and the sampling needle is arranged in the hollow structure in a penetrating way;
the method comprises the following steps:
the driving mechanism drives the puncture needle to penetrate through a test tube cover of a target test tube and drives the sample separation needle to move downwards in a hollow structure of the puncture needle;
the sample separation needle sucks serum in the target test tube;
the driving mechanism drives the sample separating needle to move upwards in the hollow structure of the puncture needle and drives the puncture needle to move upwards so as to leave the test tube cover of the target test tube;
wherein, a space is arranged between the outer wall of the sample separating needle and the inner wall of the puncture needle, and
an isolation mechanism is arranged between the outer wall of the sample separation needle and the inner wall of the puncture needle, and the isolation mechanism is configured to: the driving mechanism drives the puncture needle to pass through a test tube cover of a target test tube so as to enable the internal air pressure of the target test tube to be communicated with the atmosphere through the hollow structure when the sample separation needle sucks the serum in the target test tube, and enable the hollow structure to be isolated from the atmosphere after the sample separation needle sucks the serum in the target test tube; or alternatively
The sidewall of the puncture needle is configured to: the driving mechanism drives the puncture needle to pass through a test tube cover of a target test tube so that the internal air pressure of the target test tube is communicated with the atmosphere through the hollow structure when the sample separation needle sucks the serum in the target test tube, and the hollow structure is isolated from the atmosphere after the sample separation needle sucks the serum in the target test tube.
2. The method of claim 1, wherein the driving mechanism drives the lancet through a tube cap of a target tube, comprising:
the driving mechanism drives the puncture needle to pass through the test tube cover of the target test tube, and the lower end of the puncture needle is higher than the surface of serum in the target test tube;
the driving sample separating needle moves downwards in the hollow structure of the puncture needle, and the driving sample separating needle comprises:
the sample separation needle is driven to move downwards in the hollow structure of the puncture needle until the lower end of the sample separation needle stretches into serum in the target test tube.
3. The method of claim 2, wherein said driving the needle down in the hollow structure of the needle until the lower end of the needle protrudes into the serum in the target tube comprises:
detecting whether the lower end of the sample separation needle touches the surface of serum in the target test tube or not when the sample separation needle is driven to move downwards;
after the lower end of the sample separating needle touches the surface, the sample separating needle is driven to continuously move downwards for a first preset distance, and the first preset distance is determined according to a preset serum sucking amount.
4. The method of claim 1, wherein upon driving the sample divider upward in the hollow structure of the puncture needle, the method further comprises:
when the distance between the lower end of the sample separation needle and the surface of serum in the target test tube reaches a second preset distance, the sample separation needle sucks in isolation air or performs quantitative exhaust.
5. The method of claim 1, wherein the isolation mechanism or the side wall of the puncture needle is provided with a solenoid valve,
the solenoid valve is capable of venting or isolating the hollow structure of the spike from the atmosphere, the method further comprising:
opening the solenoid valve when the drive mechanism drives the puncture needle to pass through a test tube cover of a target test tube;
the solenoid valve is closed after the sample separation needle sucks the serum in the target test tube.
6. The method according to any one of claims 1-5, further comprising:
when the sample separation needle is driven to move upwards in the hollow structure of the puncture needle, air is blown to the outer wall of the sample separation needle, so that serum on the outer wall of the sample separation needle is blown back to the target test tube.
7. The method according to any one of claims 1-5, further comprising:
moving the target test tube to a preset test tube position, and righting and stabilizing the target test tube;
the driving mechanism drives the puncture needle and the sample dividing needle to move above the target test tube in the horizontal direction;
wherein the driving mechanism drives the puncture needle or the sample separation needle to move upwards or downwards at the test tube position.
8. The method of claim 7, wherein the method further comprises:
the driving mechanism drives the puncture needle and the sample dividing needle to move to a cleaning position in the horizontal direction;
and cleaning the puncture needle and the sample separation needle.
9. A blood sampling device, characterized in that the blood sampling device comprises:
the puncture needle is provided with a hollow structure;
the sample separating needle penetrates through the hollow structure and sucks serum in the target test tube;
the driving mechanism is connected with the sample dividing needle and the puncture needle, drives the puncture needle to penetrate through the test tube cover of the target test tube, drives the sample dividing needle to move downwards in the hollow structure of the puncture needle, drives the sample dividing needle to move upwards in the hollow structure of the puncture needle, and drives the puncture needle to move upwards to leave the test tube cover of the target test tube;
wherein, a space is arranged between the outer wall of the sample separating needle and the inner wall of the puncture needle, and
an isolation mechanism is arranged between the outer wall of the sample separation needle and the inner wall of the puncture needle, and the isolation mechanism is configured to: the driving mechanism drives the puncture needle to pass through a test tube cover of a target test tube so as to enable the internal air pressure of the target test tube to be communicated with the atmosphere through the hollow structure when the sample separation needle sucks the serum in the target test tube, and the hollow structure is isolated from the atmosphere after the sample separation needle sucks the serum in the target test tube; or alternatively
The sidewall of the puncture needle is configured to: the driving mechanism drives the puncture needle to pass through a test tube cover of a target test tube so that the internal air pressure of the target test tube is communicated with the atmosphere through the hollow structure when the sample separation needle sucks the serum in the target test tube, and the hollow structure is isolated from the atmosphere after the sample separation needle sucks the serum in the target test tube.
10. The apparatus according to claim 9, wherein a solenoid valve is provided on a side wall of the isolating mechanism or the puncture needle, the solenoid valve being capable of communicating or isolating the hollow structure of the puncture needle from the atmosphere;
the electromagnetic valve is opened when the driving mechanism drives the puncture needle to pass through the test tube cover of the target test tube; and closing the electromagnetic valve after the sample separation needle sucks serum in the target test tube.
11. The apparatus according to any one of claims 9-10, characterized in that the apparatus further comprises:
the air blowing mechanism is connected with the puncture needle;
and when the driving mechanism drives the sample separation needle to move upwards in the hollow structure of the puncture needle, the air blowing mechanism blows air to the outer wall of the sample separation needle so as to blow the serum on the outer wall of the sample separation needle back to the target test tube.
12. The apparatus according to any one of claims 9-10, characterized in that the apparatus further comprises:
the test tube transfer device is used for bearing the target test tube, moving the target test tube to a preset test tube position and righting and stabilizing the test tube;
the driving mechanism also drives the puncture needle and the sample separation needle to move above the target test tube in the horizontal direction;
wherein the driving mechanism drives the puncture needle or the sample separation needle to move upwards or downwards at the test tube position.
13. The apparatus of claim 12, wherein the apparatus further comprises:
the cleaning device is arranged at a cleaning position, and the cleaning position is adjacent to the test tube position;
the cleaning device cleans the puncture needle and the sample separation needle when the driving mechanism drives the puncture needle and the sample separation needle to move to the cleaning position in the horizontal direction.
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| CN202010501406.3A CN113759142B (en) | 2020-06-04 | 2020-06-04 | Blood sampling method and blood sampling device |
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| CN113759142A (en) | 2021-12-07 |
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