CN113588969A

CN113588969A - Full-automatic fluorescence immunoassay analyzer and sample detection method

Info

Publication number: CN113588969A
Application number: CN202010368313.8A
Authority: CN
Inventors: 许行尚; 杰弗瑞·陈
Original assignee: Nanjing Lanyu Biological Technology Co Ltd
Current assignee: Nanjing Lanyu Biological Technology Co Ltd
Priority date: 2020-04-30
Filing date: 2020-04-30
Publication date: 2021-11-02

Abstract

The invention discloses a full-automatic fluorescence immunoassay analyzer and a sample detection method, wherein the analyzer comprises a bracket, the bracket is arranged on a base, a sample introduction and sample discharge device is arranged on the base, a sample tube blending assembly is arranged on the side surface of the sample introduction and sample discharge device, and the sample tube is used for introducing and discharging samples by the sample introduction and sample discharge device; the device also comprises a sampling needle and dilution needle module which is used for extracting a sample, adding the sample into the mixing reaction tank, adding a diluent into the mixing reaction tank for dilution and mixing, and adding the diluted and mixed sample into the reagent card by the sampling needle; a reagent card bin assembly is arranged in the support, a plurality of reagent cassettes are arranged in the reagent card bin assembly, and reagent cards are conveyed through the reagent card carrying assembly; a detection assembly for detecting a reagent card is also arranged in the bracket; the device also comprises a discarded reagent card discarding and shifting device which is used for shifting the reagent card after the detection is finished to the waste receiving barrel. The analyzer is reasonable in overall layout, complete in function and capable of achieving full-automatic operation.

Description

Full-automatic fluorescence immunoassay analyzer and sample detection method

Technical Field

The invention belongs to the field of fluorescence immunoassay detection, and particularly relates to a full-automatic fluorescence immunoassay analyzer and a sample detection method.

Background

The fluorescence immunoassay technology is a detection technology commonly used in biomedical detection at present, and the fluorescence immunoassay technology is characterized in that the analyte is qualitatively and quantitatively detected by utilizing the characteristic that the analyte generates fluorescence under the excitation of specific wavelength light. The fluorescence immunoassay technology has a series of advantages of high sensitivity, strong specificity, high detection speed, safety, stability and the like, so the fluorescence immunoassay technology is widely applied to clinical detection and has wide application prospect in the detection fields of endocrine disease detection, infectious disease detection, gynecological disease detection, tumor marker detection, genetic disease detection, blood and cytology detection and the like.

The traditional fluorescence immunoassay analyzer is usually large in size and inconvenient to use in a handheld manner, so that the development of the traditional fluorescence immunoassay analyzer in the field of emergency detection (such as emergency laboratories, ICUs, pediatrics, 120 emergency vehicles, outdoor emergency rescue and individual rescue), inconvenient traffic and economically undeveloped areas is limited. Even some commercial products have the defects of single detection wave band, complex structure, poor reliability, low precision and the like.

Chinese patent document CN106198480A discloses an automatic dry fluorescence detector, which includes: the sample in-and-out mechanism comprises a sample transmission device, a test tube rack capable of placing a plurality of sample test tubes and a test tube rack placing plate, wherein the test tube rack is arranged on the test tube rack placing plate, and can convey the sample test tubes from a to-be-detected position to a sampling position under the driving of the sample transmission device; the sampling mechanism comprises a sampling device, a liquid transfer device and a buffer area for storing a plurality of buffer liquid boxes, and the sampling device is driven by the liquid transfer device to switch back and forth among a sampling position, the buffer liquid boxes and a sample loading position; the rotary table mechanism comprises a rotary table and a rotary table driving device for driving the rotary table to rotate, wherein the rotary table is provided with a plurality of card carrying tongues for containing reagent cards along the radial direction, one end of each card carrying tongue extends to the edge of the rotary table, the rotary table is driven by the rotary table driving device to rotate so that the card carrying tongues reach different angles and are positioned at a sample loading position, a detection position, a lost card position and an advanced card position, and at least two advanced card positions are provided; the card feeding mechanism comprises a card feeding device, a swinging device and at least two card boxes, wherein each card box is arranged on the periphery of the rotary table along the circumferential direction, an outlet of each card box can be opposite to one card feeding position, so that a card feeding position is formed at the corresponding position of each card box, the card feeding device is arranged on the periphery of the rotary table and can rotate coaxially with the rotary table, the swinging device is connected with the card feeding device and drives the card feeding device to rotate to different card feeding positions, and the card feeding device also comprises a card poking component capable of poking reagent cards in the card boxes into the card feeding positions along the radial direction of the rotary table; the detection mechanism comprises a detection head for carrying out data acquisition on the reagent card at the detection position.

The automatic dry fluorescence detector in the technical scheme has the advantages that the effect is not ideal in the actual use process, the humanized design is poor, and the function is deficient, so that the existing fluorescence immunoassay device needs to be improved, a new full-automatic fluorescence immunoassay analyzer and a sample detection method are developed, the functions are complete, the full-automatic operation is realized, and the detection efficiency of the reagent card is greatly improved.

Disclosure of Invention

The invention aims to provide a full-automatic fluorescence immunoassay analyzer.

In order to solve the technical problems, the invention adopts the technical scheme that the full-automatic fluorescence immunoassay analyzer comprises a bracket, wherein the bracket is arranged on a base, a sample feeding device and a sample discharging device are arranged on the base, a sample tube blending assembly is arranged on the side surface of the sample feeding device and the sample discharging device and is used for blending samples in a sample tube, and the sample tube is pushed in by the sample feeding device and the sample discharging device to be fed in and pushed out to carry out sample discharging;

the device also comprises a sampling needle and dilution needle module which is used for extracting a sample in a sample tube, adding the sample into a mixing reaction tank, adding a diluent into the mixing reaction tank for dilution and mixing, wherein the sampling needle and dilution needle module comprises a sampling needle and a dilution needle, and the sampling needle adds the diluted and mixed sample into a sample adding port of the reagent card;

a reagent card bin assembly is arranged in the support, and a plurality of reagent cassettes are arranged in the reagent card bin assembly;

a reagent card carrying assembly is further arranged in the support and used for conveying reagent cards;

a detection assembly for detecting the reagent card is also arranged in the bracket;

the device also comprises a discarded reagent card discarding and shifting device which is used for shifting the reagent card after the detection is finished to the waste receiving barrel.

The full-automatic fluorescence immunoassay analyzer disclosed by the invention has the advantages that all parts are arranged in a centralized manner, so that the whole layout of the analyzer is reasonable and compact, the functions are complete, and the full-automatic operation is realized; the sample tube mixing component realizes the mixing function, a plurality of reagent cassettes are stacked in the reagent cassette bin component, a plurality of reagent cards are arranged in the reagent cassettes, and the cassette loading is performed once, so that the continuous detection can be performed for a plurality of times; the detection assembly is positioned in the support and used for detecting a fluorescence reaction signal of the reagent card, and the kit is comprehensive in function, humanized in design, efficient and rapid.

Preferably, the sample tube blending assembly comprises a sample tube up-and-down movement sliding block assembly, a sample tube up-and-down movement motor, a sample tube up-and-down movement sliding rail, a sample tube clamping jaw movement assembly, a sample tube clamping jaw movement motor, a sample tube swinging movement motor and a sample tube swinging fixing block, wherein the sample tube clamping jaw movement motor is connected with the sample tube clamping jaw movement assembly, and the sample tube clamping jaw movement assembly is connected with the sample tube clamping jaw; the sample tube clamping jaw moving assembly is connected with the sample tube swinging fixing block and the sample tube up-and-down moving sliding block assembly, and the sample tube swinging moving motor and the sample tube up-and-down moving sliding block assembly are connected with the sample tube swinging fixing block; the sample tube up-and-down movement motor and the sample tube up-and-down movement sliding rail are connected with the sample tube up-and-down movement sliding block component. The sample tube clamping jaws clamp the sample tube, the sample tube up-and-down movement motor drives the sample tube up-and-down movement sliding block assembly connected with the sample tube clamping jaw movement assembly to move up and down along the sample tube up-and-down movement sliding rail, so that the sample tube clamping jaws connected with the sample tube up-and-down movement sliding block assembly move up and down along the sample tube up-and-down movement sliding rail, and the sample tube clamping jaws are driven to clamp the sample tube to move up and down; the sample tube swinging motor drives the sample tube swinging fixing block to move and drives the sample tube clamping jaw moving assembly connected with the sample tube swinging fixing block to swing so as to drive the sample tube clamping jaw to clamp the sample tube for swinging and uniform mixing; and the sample tube clamping jaw movement motor drives the sample tube clamping jaw movement assembly to control the sample tube clamping jaw to clamp and loosen the sample tube. The sample tube up-and-down movement motor is a screw rod motor; the side surface of the sample tube up-and-down movement motor is provided with an up-and-down movement optical coupler, and correspondingly, the sample tube up-and-down movement sliding block component is provided with an up-and-down movement sensing chip; the moving position of the screw rod motor is controllable and accurate; the sensing chip is matched with the optocoupler to control the up-and-down moving distance of the swing arm; the opto-coupler cooperates with the sense piece, and when the sense piece moved to the opto-coupler department, the opto-coupler sensed the signal change, and the motion motor stopped working promptly. And the sample tube clamping jaw movement motor and the sample tube swinging movement motor are both stepping motors. The switching of level and upper and lower direction for sample pipe mixing subassembly can accurately snatch the promotion sample pipe rapidly, prepares for the swing mixing.

Preferably, the sample tube up-and-down movement sliding block assembly comprises a sample tube up-and-down sliding block assembly and a sample tube up-and-down fixing block, the sample tube up-and-down sliding block assembly is connected with the sample tube up-and-down movement sliding rail and moves up and down along the sample tube up-and-down movement sliding rail, and the sample tube up-and-down fixing block is connected with the sample tube up-and-down movement motor through a sample tube up-and-down movement screw rod.

Preferably, the sample tube clamping jaw movement assembly comprises a clamping jaw fixing plate, a clamping jaw sliding groove, a clamping jaw sliding block, a clamping jaw connecting rod and a sample tube clamping jaw motor shaft fixing block, the sample tube clamping jaw motor shaft fixing block is connected with the sample tube clamping jaw movement motor, the sample tube clamping jaw is connected with the clamping jaw fixing plate, the clamping jaw fixing plate is connected with the sample tube clamping jaw motor shaft fixing block through the clamping jaw connecting rod, and the clamping jaw fixing plate is movably connected with the clamping jaw connecting rod; the clamping jaw sliding groove is connected with a sample tube clamping jaw motor shaft fixing block, and the clamping jaw fixing block is connected with the clamping jaw sliding groove through the clamping jaw sliding block and moves horizontally in the clamping jaw sliding groove, so that a sample tube is clamped and loosened by a sample tube clamping jaw.

Preferably, the sample tube clamping jaw comprises a first clamping jaw and a second clamping jaw, correspondingly, the clamping jaw connecting rod comprises a first clamping jaw connecting rod and a second clamping jaw connecting rod, the clamping jaw fixing plate comprises a first clamping jaw fixing plate and a second clamping jaw fixing plate, the clamping jaw sliding block comprises a first clamping jaw sliding block and a second clamping jaw sliding block, one side of the first clamping jaw is connected with the first clamping jaw connecting rod through the first clamping jaw fixing plate, and the first clamping jaw fixing plate is fixedly connected to the first clamping jaw sliding block; one side of the clamping jaw II is connected with the clamping jaw connecting rod II through the clamping jaw fixing piece II, and the clamping jaw fixing piece II is fixedly connected to the clamping jaw sliding block II; the first clamping jaw sliding block and the second clamping jaw sliding block are connected with the clamping jaw sliding groove and move horizontally in the clamping jaw sliding groove, so that the first clamping jaw and the second clamping jaw simultaneously move inwards or outwards horizontally, and a sample tube is clamped and loosened.

Preferably, the sampling needle and dilution needle module comprises a dilution needle up-and-down movement assembly, a sampling needle up-and-down movement assembly and a sampling needle and dilution needle horizontal movement assembly;

the dilution needle up-and-down movement assembly comprises a dilution needle up-and-down movement motor and a dilution needle up-and-down movement sliding block assembly, the dilution needle is arranged on the dilution needle up-and-down movement sliding block assembly, and the dilution needle up-and-down movement motor drives the dilution needle up-and-down movement sliding block assembly to reciprocate along a dilution needle up-and-down sliding rail;

the sampling needle up-and-down movement assembly comprises a sampling needle up-and-down movement motor and a sampling needle up-and-down movement sliding block assembly, the sampling needle is arranged on the sampling needle up-and-down movement sliding block assembly, and the sampling needle up-and-down movement motor drives the sampling needle up-and-down movement sliding block assembly to reciprocate along a sampling needle up-and-down sliding rail;

sampling needle and diluter horizontal motion subassembly are including sampling needle and diluter horizontal motion motor and sampling needle and diluter horizontal motion sliding block set spare, sampling needle up-and-down motion subassembly with diluter up-and-down motion subassembly is all installed on the fixed riser of sampling needle and diluter horizontal motion sliding block set spare, sampling needle and diluter horizontal motion motor drive sampling needle and diluter horizontal motion sliding block set spare reciprocating motion on sampling needle and diluter horizontal slide rail.

The sampling needle and the dilution needle can be accurately inserted into the sample tube to sample and take out the dilution liquid through the sampling needle up-and-down movement assembly, the dilution needle up-and-down movement assembly, the sampling needle and the dilution needle horizontal movement assembly to switch the levels and the up-and-down directions of the sampling needle and the dilution needle. The mode of uniformly mixing the sample and the diluent is that the sample and the diluent are respectively added into a uniformly mixing reaction tank through a sampling needle and a diluent needle, and then the uniformly mixed diluted sample is absorbed by the sampling needle and added into a reagent card. The reagent card detection device is simple in structure, saves cost, is easy to control in a programmed mode, is high in automation degree, and greatly improves the reagent card detection efficiency.

Preferably, the sampling needle motion assembly further comprises a guide bending belt, one end of the guide bending belt is fixed above the fixed vertical plate, the other end of the guide bending belt is arranged on the sampling needle motion assembly support frame, and the guide bending belt is arranged along the horizontal guide rail of the sampling needle in a longitudinal mode.

The guide bending belt further stabilizes a sampling needle movement assembly, so that the sampling needle does not shake transversely when moving horizontally, and sampling is accurate; in addition, the inside of the guide bending belt is provided with a circuit or a liquid pipeline, so that the circuit or the liquid pipeline is not exposed and is neat and attractive.

Preferably, the sampling needle and dilution needle module further comprises a diluent bin assembly, the diluent bin assembly comprises a diluent bin, a diluent bin movement motor, a diluent bin slider, a diluent bin slide rail and a diluent bin movement assembly, the diluent bin movement motor is connected with the diluent bin movement assembly, and the diluent bin slider is respectively connected with the diluent bin and the diluent bin slide rail, so that the diluent bin movement motor drives the diluent bin to reciprocate along the diluent bin slide rail; the diluent bin is positioned below the diluent needle and is used for placing a plurality of diluent bottles; and a blending reaction tank is arranged on the side surface of the diluent bin. The dilution needle can obtain the dilution from the dilution liquid bottle through a pipeline and inject the dilution into the blending reaction tank.

Preferably, the diluent bin movement assembly comprises a diluent bin driving wheel, a diluent bin driven wheel and a diluent bin belt, the diluent bin movement motor is a stepping motor, the diluent bin movement motor is connected with the diluent bin driving wheel, the diluent bin belt is sleeved on the diluent bin driving wheel and the diluent bin driven wheel, and the diluent bin sliding block is sleeved on the diluent bin belt.

Preferably, the sampling needle up-and-down movement motor and the dilution needle up-and-down movement motor are both screw rod motors, and the sampling needle up-and-down movement sliding block assembly is sleeved on a sampling needle up-and-down movement motor screw rod connected with the sampling needle up-and-down movement motor; the diluent needle up-and-down movement sliding block assembly is sleeved on a diluent needle up-and-down movement motor screw rod connected with the diluent needle up-and-down movement motor; the sampling needle is step motor with dilution needle horizontal motion motor, the sampling needle is connected with sampling needle and dilution needle horizontal motion action wheel with dilution needle horizontal motion motor, sampling needle and dilution needle horizontal motion action wheel are connected with sampling needle and dilution needle horizontal motion from the driving wheel through sampling needle and dilution needle horizontal motion belt, sampling needle and dilution needle horizontal motion sliding block assembly cup joint on sampling needle and the dilution needle horizontal motion belt, just sampling needle and dilution needle horizontal sliding block assembly with sampling needle and dilution needle horizontal slide rail sliding connection. The sampling needle and dilution needle horizontal movement motor drives the sampling needle and dilution needle horizontal movement driving wheel to rotate, and the sampling needle and dilution needle horizontal movement belt sleeved on the sampling needle and dilution needle horizontal movement driving wheel drives the sampling needle and dilution needle horizontal movement driven wheel to rotate, so that the sampling needle and dilution needle horizontal movement sliding block assembly horizontally moves along the sampling needle and dilution needle horizontal movement sliding rail; the fixed vertical plate is connected with the sampling needle and the diluting needle horizontal movement belt through the sampling needle and diluting needle horizontal movement sliding block component.

Preferably, a sampling needle up-and-down movement limiting optocoupler is arranged on the fixed vertical plate and positioned at the upper end of the sampling needle up-and-down sliding rail; on sampling needle and the dilution needle motion subassembly support frame, and be located and be close to sampling needle and dilution needle horizontal guide's end afterbody is provided with the spacing opto-coupler of sampling needle and dilution needle horizontal motion.

Preferably, the reagent card chamber assembly comprises a reagent card chamber, and a plurality of reagent card chamber partitions are arranged in the reagent card chamber to enable the reagent card chamber to have a plurality of mutually independent reagent card chamber grooves for placing the reagent card chambers; the reagent card bin partition is fixed through the bottom fixing block and the top fixing block. The reagent card storehouse cuts off and is the arc, and its inside reagent card casket of placing, its top, bottom are fixed spacing by top fixed block and bottom fixed block respectively, stable in structure.

Preferably, a reagent card detector is arranged on the inner side wall of the reagent card bin and used for detecting the number of the reagent cards; the reagent card detector is arranged on the side wall of the reagent card bin, can detect the number of the reagent cards and remind users of adding the reagent cards in time; 20-30 reagent cards can be placed in one reagent cassette, can be placed according to detection items and can be configured according to the required quantity.

Preferably, the reagent card bin assembly further comprises a plurality of reagent card hand-shifting devices, each reagent card hand-shifting device is provided with a reagent card hand-shifting motor, and the reagent card hand-shifting motor drives a reagent card hand-shifting connecting block fixedly connected with a reagent card hand-shifting belt to reciprocate along a reagent card hand-shifting sliding rail through a reagent card hand-shifting driving wheel, a reagent card hand-shifting driven wheel and the reagent card hand-shifting belt; the reagent card hand-shifting device comprises a reagent card hand-shifting device and a reagent card hand-shifting device, wherein a reagent card hand-shifting connecting block is arranged on the reagent card hand-shifting device, the reagent card hand-shifting device also comprises a reagent card hand-shifting motion plate, the reagent card hand-shifting motion plate is positioned at the bottom of a reagent card bin, reagent card hand-shifting motion tracks with the number corresponding to that of the reagent card slot penetrate through the reagent card hand-shifting motion plate, and the reagent card hand-shifting device shifts reagent cards in the reagent card box to move to a reagent card carrying component along the reagent card hand-shifting motion tracks after penetrating through the reagent card hand-shifting motion tracks; reagent card motion is set up the spacing wall of reagent card motion on the reagent card thumb wheel motion track, reagent card motion is spacing to be located the below of the top fixed block riser in reagent card storehouse.

After the reagent card shifting hand moves to the reagent card carrying track, the reagent card shifting hand moves backwards to an initial position immediately, the head of the shifting hand is pressed down under the action of a spring (elastic piece) and an upper pressing plate, and when the shifting hand continues to shift the reagent card to the carrying track, the head of the shifting hand bounces to penetrate through the reagent card moving track to push the reagent card to move to the carrying track along the reagent card moving track.

The reagent cassette push-in-place sensor detects whether the reagent cassette is pushed in place or not so as to be matched with other components, particularly a reagent card shifting hand device, which needs to shift a reagent card in a reagent card bin onto a reagent card carrying track in a support, so that the position requirement of the reagent cassette is strict.

Preferably, the reagent card carrying assembly comprises a reagent card transfer mechanism and a carrying track, wherein a reagent card in a reagent cassette in the reagent card bin is firstly dialed to the carrying track by a reagent card hand, then dialed to the reagent card transfer mechanism by a carrying hand dialing device, and then dialed to the detection assembly of the reagent card by a carrying hand dialing device. After the reagent card pusher pushes the reagent card to the carrying track, the head of the reagent card pusher is pushed down to move backwards under the action of the gravity of the reagent card in the reagent card box when the reagent card pusher moves backwards, and then the next reagent card is pushed to the carrying track.

Preferably, the reagent card transferring mechanism comprises a transferring motor and a reagent card transferring bin, the reagent card transferring bin is connected with the transferring motor, and the reagent card transferring bin is driven by the transferring motor to horizontally rotate; the carrying shifting hand device comprises a carrying shifting hand I, a carrying shifting hand I motor, a carrying shifting hand I motion assembly, a carrying shifting hand I slide block assembly and a carrying shifting hand I slide rail, wherein the carrying shifting hand I is connected with the carrying shifting hand I slide block assembly, the carrying shifting hand I slide block assembly is sleeved on the carrying shifting hand I slide rail and the carrying shifting hand I motion assembly, the carrying shifting hand I motor is connected with the carrying shifting hand I motion assembly, and the carrying shifting hand I motion assembly is driven by the carrying shifting hand I motor to drive the carrying shifting hand I slide block assembly to move so as to drive the carrying shifting hand I connected with the carrying shifting hand I slide block assembly to reciprocate along the carrying shifting hand I slide rail; the second carrying shifting hand device comprises a second carrying shifting hand, a second motor for the carrying shifting hand, a second moving assembly for the carrying shifting hand, a second sliding block assembly for the carrying shifting hand and a second sliding rail for the carrying shifting hand, the second carrying shifting hand is connected with the second sliding block assembly for the carrying shifting hand, the second sliding block assembly for the carrying shifting hand is sleeved on the second sliding rail for the carrying shifting hand and the second moving assembly for the carrying shifting hand, the second motor for the carrying shifting hand is connected with the second moving assembly for the carrying shifting hand, and the second moving assembly for the carrying shifting hand is driven by the second motor for the carrying shifting hand to drive the second carrying shifting hand connected with the second sliding block assembly for the carrying shifting hand to reciprocate along the second sliding rail for the carrying shifting hand. The first carrying shifting handle moving assembly comprises a first carrying shifting handle driving wheel, a first carrying shifting handle driven wheel and a first carrying shifting handle belt, the first carrying shifting handle belt is sleeved between the first carrying shifting handle driving wheel and the first carrying shifting handle driven wheel, the first carrying shifting handle driving wheel is connected with a first carrying shifting handle motor, and the first carrying shifting handle sliding block assembly is sleeved on the first carrying shifting handle belt; the second conveying shifting handle movement assembly comprises a second conveying shifting handle driving wheel, a second conveying shifting handle driven wheel and a second conveying shifting handle belt, the second conveying shifting handle belt is sleeved between the second conveying shifting handle driving wheel and the second conveying shifting handle driven wheel, the second conveying shifting handle driving wheel is connected with a second conveying shifting handle motor, and the second conveying shifting handle sliding block assembly is sleeved on the second conveying shifting handle belt.

Preferably, the reagent card hand and the carrying hand both comprise hand-shifting heads, and elastic parts are arranged below the hand-shifting heads; the reagent card shifting hand is also provided with a shifting hand tail part, the shifting hand tail part is movably connected with a shifting hand sliding block component (a reagent card shifting hand connecting block and a carrying shifting hand two-sliding block component), and the shifting hand head part is connected with the shifting hand tail part through an inclined part. The first carrying finger is preferably in a strip shape similar to the shape of the reagent card, but is not limited to the shape.

Preferably, the detection assembly comprises a reagent card detection disc and an optical detection module, the optical detection module is mounted on a support plate through a detection support, and a lighting head of the optical detection module is connected with the detection support through a connecting arm, so that the lighting head is arranged above the reagent card detection disc and corresponds to the position of a reagent card detection window on the reagent card detection disc, and therefore the lighting head collects optical signals for the reagent card detection window positioned below the lighting head; and the top of the light collection head is also provided with a shielding cover. The bottom of the reagent card detection disc is provided with a constant-temperature heating module for heating the reagent card and keeping the reagent card at a constant temperature. The structure arrangement can directly collect and process fluorescence signals of the reagent card detection window without moving, the structure of the originally designed optical detection module assembly is complex, and the movement is controlled by two motors to detect the result of the reagent card, so that the detection efficiency of the reagent card is greatly improved.

Preferably, the reagent card detection tray is connected with a reagent card detection tray rotating motor for driving the reagent card detection tray to rotate; a plurality of reagent screens are uniformly distributed on the reagent card detection plate and used for placing reagent cards. The reagent card detection disc rotating motor is connected with the reagent card detection disc driving wheel, the reagent card detection disc is connected with the reagent card detection disc driven wheel, and the reagent card detection disc belt is sleeved between the reagent card detection disc driving wheel and the reagent card detection disc driven wheel, so that the reagent card detection disc is driven to rotate by the reagent card detection disc rotating motor.

Preferably, the reagent card discarding hand-dialing device is arranged at the outer side of the reagent card detection disc and at a position corresponding to the lighting head, and comprises a reagent card discarding hand-dialing, a reagent card discarding hand-dialing motor, a reagent card discarding hand-dialing sliding block component, a reagent card discarding hand-dialing sliding rail and a reagent card discarding hand-dialing motion component, wherein the reagent card discarding hand-dialing is connected with the reagent card discarding hand-dialing sliding block component, the reagent card discarding hand-dialing motor is connected with the reagent card discarding hand-dialing motion component, the reagent card discarding hand-dialing sliding block component is sleeved on the reagent card discarding hand-dialing motion component and the reagent card discarding hand-dialing sliding rail, and the reagent card discarding hand-dialing motor drives the reagent card discarding hand-dialing motion component to drive the reagent card discarding hand-dialing component to move, so as to drive the reagent card discarding hand-dialing connected with the reagent card discarding hand-dialing component to discard along the reagent card discarding hand-dialing The slide rail reciprocates. The reagent card after the detection is finished is abandoned, and the hand is dialed to the waste receiving barrel, so that the detection efficiency of the reagent card is greatly improved. The reagent card abandoning hand shifting motion assembly comprises a reagent card abandoning hand shifting driving wheel, a reagent card abandoning hand shifting driven wheel and a reagent card abandoning hand shifting belt, the reagent card abandoning hand shifting belt is sleeved between the reagent card abandoning hand shifting driving wheel and the reagent card abandoning hand shifting driven wheel, the reagent card abandoning hand shifting driving wheel is connected with a reagent card abandoning hand shifting motor, and the reagent card abandoning hand shifting sliding block assembly is sleeved on the reagent card abandoning hand shifting belt. The structure of the reagent card disposal hand is the same as that of the reagent card hand.

Preferably, reagent card clamping pieces are arranged on two sides of the reagent card position, and are used for clamping two sides of the reagent card to fix the reagent card in the reagent card detection tray.

Preferably, a reagent card code scanner is arranged above the reagent card transfer bin; and a cleaning assembly is arranged above the first carrying shifting hand, and a plurality of cleaning liquid bottles are placed on the cleaning assembly through a cleaning liquid bottle support. The reagent card code scanner is used for scanning the reagent card positioned on the reagent card transfer bin, acquiring the information of the reagent card and confirming that the sample detection item is correct; the cleaning liquid is used for cleaning the sampling needle and the dilution needle.

Preferably, the sample feeding and discharging device comprises a sample feeding pushing handle structure, a sample feeding transverse pushing and poking handle structure and a sample discharging pushing handle structure; the sample pushing handle structure is used for pushing a sample tube placed on a sample platform of the sample inlet and outlet platform into a sample along the longitudinal direction; the sample introduction transverse pushing and poking hand structure is used for pushing the sample tube into a sample introduction along the transverse direction; the sample outlet pushing hand structure is used for pushing the detected sample tube out of the sample outlet platform of the sample inlet and outlet platform to carry out sample outlet. The sample inlet and outlet platform comprises a sample inlet platform and a sample outlet platform, and a plurality of test tube rack positioner mounting ports are formed in the side surface of the sample inlet and outlet platform; a sample tube transverse pushing and poking hand outlet is arranged on the sample introduction platform; and a sample outlet pushing hand outlet is arranged on the side surface of the sample outlet platform.

Preferably, the sample injection pushing handle structure is provided with a sample injection pushing handle, and the sample injection pushing handle is arranged on the first guide rail through a first sliding block assembly; the first sliding block assembly is fixed on the first belt, the first motor drives the first sliding block assembly to longitudinally reciprocate along the first guide rail through the first driving wheel, the first driven wheel and the first belt, and then the sample tube is longitudinally pushed into a sample.

The sample injection pushing hand structure is used for pushing a sample tube placed on the sample injection platform in the longitudinal direction, and is matched with the sample injection transverse pushing hand structure to work so as to further push the sample tube in the transverse direction for sample injection; by adopting the structure, the first sliding block assembly is fixed on the first belt, and the first sliding block assembly is driven to longitudinally reciprocate along the first guide rail by the movement of the first belt, so that the structure is simple, and the response is rapid; and the guide rail I is used for guiding, the moving direction of the sample tube is stable, and the error rate is low.

Preferably, the sample outlet pushing hand structure is provided with a sample outlet pushing hand, and the sample outlet pushing hand is arranged on the third guide rail through a third sliding block assembly; and the third sliding block assembly is fixed on a third belt, and a third motor drives the third sliding block assembly to longitudinally reciprocate along the third guide rail through a third driving wheel, a third driven wheel and the third belt, so that the detected sample tube is pushed out to the sample outlet platform for sample outlet.

Preferably, the sample introduction transverse pushing and poking hand structure is provided with a plurality of transverse poking hands, and the transverse poking hands are arranged on the second guide rail through the second sliding block assembly; the second sliding block assembly is fixed on a second belt, and a second motor drives the second sliding block assembly to transversely reciprocate along the second guide rail through a second driving wheel, a second driven wheel and the second belt, so that the sample tube is transversely pushed into a sample injection way; the sample tube is placed on a test tube rack with a plurality of test tube rack positioning grooves, and the distance between two adjacent transverse shifting hands is not more than the length of the test tube rack; the bottom of test-tube rack is equipped with a plurality of bottoms and indulges the spacer block, every two the bottom is indulged the spacer block and is separated by the position of a test tube.

In the invention, the structures of the sample injection transverse pushing hand driver and the mixing reaction tank hand driver are the same and are both provided with hand driver heads, an elastic part (generally a spring) is arranged below the hand driver heads, the hand driver tails are movably connected with the sliding block components, and the hand driver heads are connected with the hand driver tails through inclined parts; the transverse shifting hand movement drives the sliding block component through the starting of the motor, then drives the transverse shifting hand to move, one sample tube position of the test tube rack is advanced once, then the transverse shifting hand moves backwards, the head part of the shifting hand is pressed downwards and moves backwards under the action of the bottom longitudinal partition block of the test tube rack, then the test tube rack is advanced by one sample tube position under the action of the motor, and the test tube rack is pushed to advance through the repeated movement; the distance between two adjacent transverse shifting hands is not larger than the length of the test tube rack, and the transverse shifting hands are guaranteed to push the bottom of the test tube rack all the time.

Preferably, the sample feeding and discharging device further comprises a transverse movement track, and the test tube rack feeds and discharges samples along the transverse movement track.

Set up the lateral motion track for the test-tube rack advances kind and goes out the appearance and all orderly regular, has optimized overall structure.

Preferably, the full-automatic fluorescence immunoassay analyzer further comprises an emergency treatment sample placing position, wherein the emergency treatment sample placing position is used for placing the sample tube and is provided with an emergency treatment detection position and an emergency treatment sample tube detection sensor; the emergency sample placing position is located in the horizontal movement direction of the sampling needle.

The emergency detection position is used for starting an emergency detection process; the emergency treatment sample tube detection sensor is used for detecting whether the emergency treatment sample tube is placed in place or not and then continuing the detection process; place the setting with emergency call sample on the horizontal motion direction of sampling needle, like this, the sampling needle that horizontal migration came can be accurate stretch into emergency call sample pipe and take a sample.

Preferably, a cleaning pool is arranged on one side of the emergency detection position and used for cleaning the sampling needle; the sample introduction platform and the emergency detection position are provided with a sample tube code scanner assembly for scanning the information of the sample tube for sample introduction.

Preferably, the full-automatic fluorescence immunoassay analyzer is provided with a test tube rack detection sensor, a test tube rack positioner and a sample tube detection sensor at the rear side of the sample introduction and discharge device; the test tube rack detection sensor is installed on a vertical plate of the support, and the test tube rack positioner and the sample tube detection sensor sense through corresponding windows of the vertical plate of the support.

The test tube rack detection sensor is used for detecting whether the test tube rack is pushed to a preset position or not; the test tube rack positioner is used for positioning; the sample tube detection sensor is used for detecting whether the sample tube is in a preset position.

Preferably, a sample code scanner is arranged above the sample tube detection sensor; a sample code scanning rotating mechanism is arranged close to the sample code scanner and comprises a sample tube rotating motor, and the sample tube rotating motor drives a rotating wheel to rotate; sweep a yard rotary mechanism still including runner one and runner two, runner one and runner two all are connected with the tensioning spring, runner one and runner two with have the clearance between the swiveling wheel.

The reagent rack positioner and the positioning groove are arranged because the test tube rack is light, and the reagent rack positioner and the positioning groove are matched to enable the test tube rack to be pressed and moved on the transverse movement track by being stirred by a stirring handle without being suspended; sample pipe rotating electrical machines drives swiveling wheel rotary motion, and the sample pipe is blocked between swiveling wheel and two runners (respectively connect a spring), because the rotation of swiveling wheel and two runners are blocked under the pressure combined action under the spring action and slowly rotate, when the sample pipe is rotatory to the bar code when facing sample bar code scanner, sample bar code scanner sweeps the sign indicating number back, sweeps a sign indicating number rotating electrical machines stop work, and the sample pipe continues to be dialled the hand and drives the motion.

Gaps are reserved between the first rotating wheel and the second rotating wheel and the rotating wheel, and after the rotating wheel acts, the sample tube is screwed into the gaps between the first rotating wheel, the second rotating wheel and the rotating wheel and is clamped by the tensioning spring to rotate until the sample code scanner scans codes successfully.

Preferably, the support is further covered with a shell outside, and a display screen is arranged on the shell; the side surface of the shell is also provided with a cleaning liquid bottle inlet, a reagent cassette, a diluting liquid bottle inlet and a waste receiving barrel, the full-automatic fluorescence immunoassay analyzer is also provided with a built-in printer, and a paper outlet of the built-in printer is arranged on the shell; the shell is also provided with an observation window and an item code scanner.

The display screen is used for displaying content related to detection, and the analyzer can be operated through the display screen (touch); the cleaning liquid bottle is conveyed to the cleaning liquid bottle bracket through the cleaning liquid bottle storage inlet, the waste receiving barrel is used for collecting the detected reagent card, and the reagent card box and the diluent liquid bottle storage inlet are used for placing the reagent card box and the diluent liquid bottle; the built-in printer is used for printing the detection result out of paper; the item scanner functions as: inputting the code scanning of a new detection item, so that the code scanning information of a reagent card at the later stage is checked conveniently; updating reagent batch numbers, inputting information details including production validity periods, monitoring reagent use validity and the like.

The sample detection method comprises a normal detection process, wherein the normal detection process comprises the following steps:

s1: turning on a power switch, and waiting for the instrument to be initialized and ready;

s2: after the project is imported, a test tube rack with a sample tube is placed, a reagent card is placed in a reagent card box and is placed in a reagent card bin, a diluent bottle is placed, and patient information, sample information and the project to be detected are recorded;

s3: clicking a detection button, pushing the test tube rack to a transverse motion track, pushing the test tube rack to a sample tube code scanning position after detecting that the test tube rack is in place, starting a sample code scanner after detecting that a sample tube exists at the hole position of the test tube rack, and slowly rotating the sample tube to enable a bar code on the sample tube to face the sample code scanner, and uploading data after the sample code scanner scans the code;

s4: after the sample information is checked to be correct, the reagent card is pulled out from the reagent card bin corresponding to the detection item, when the reagent card reaches the carrying track, the reagent card is pulled to the reagent card transfer bin by the carrying pulling hand, and the two-dimensional code of the reagent card is scanned by the reagent card scanner, and then data is uploaded;

s5: after the information of the reagent card is checked to be correct, the transfer bin of the reagent card horizontally rotates to the direction of the reagent card detection plate, and the reagent card is transferred into the reagent card detection plate by the second carrying transfer hand to be added with sample;

s6: the test tube rack is shifted to a sample tube mixing position, the sample tube is clamped by the sample tube clamping jaws and is subjected to up-and-down movement and swinging movement to uniformly mix the sample in the sample tube;

s7: after the mixing is finished, inserting the sample tube back into the test tube rack, and shifting the test tube rack to a sampling position;

s8: moving the sampling needle to the position right above the sample tube, descending the sampling needle to enable the sampling needle to be inserted into the sample tube, extracting a sample in the sample tube through the sampling needle, lifting the sampling needle after sampling the sample, moving the sampling needle to a mixing reaction tank, and descending the sampling needle to add the sample into the mixing reaction tank;

s9: moving the diluent needle above the diluent bottle, descending the diluent needle, sucking the diluent, lifting the diluent needle, moving the diluent needle to the mixing reaction tank, descending the diluent needle, and adding the diluent into the mixing reaction tank;

s10: after dilution is completed, the sampling needle is moved to the position right above the blending reaction tank, the end part of the sampling needle is lowered to the blending reaction tank, and a diluted sample is extracted into the sampling needle;

s11: lifting the sampling needle, moving the sampling needle to a sample adding position of the reagent card detection disc, lowering the sampling needle to a position above a sample adding port of the reagent card on the sample adding position, and driving a diluted sample in the sampling needle into the sample adding port of the reagent card;

s12: starting the heating module, and keeping the reagent card at a set temperature for incubation; after the incubation reaction is finished, rotating the reagent card to be detected to the lower part of a lighting head of the optical detection module through the rotation of the reagent card detection disc, and collecting optical signals;

s13: after the detection is finished, the test tube rack is pushed to a sample outlet position, the test tube rack is pushed to a sample outlet platform, and the detected reagent card is pushed to a waste receiving barrel;

s14: and (5) finishing the detection program, and viewing the detection result on the instrument display screen and printing the result by using the built-in printer.

23. The method for testing a specimen according to claim 22, further comprising an emergency testing procedure, wherein the emergency testing procedure comprises the steps of:

(1) turning on a power switch, and waiting for the instrument to be initialized and ready;

(2) after the project is imported, a reagent card is put in, a diluent bottle is put in, and the patient information, the sample information and the project to be detected are recorded; (3) opening an emergency sample placing position, inserting an emergency sample tube into an emergency sample detection position, and starting a detection process after detecting that the emergency sample is inserted; the reagent card is pulled out from the reagent card bin and carried to a reagent card detection plate, a sampling needle dilution needle module is started to sample the sample to a reagent card sample adding port, a heating module is started to keep the reagent card in a set temperature for incubation; after the incubation reaction is finished, rotating the reagent card to be detected to the lower part of a lighting head of the optical detection module through the rotation of the reagent card detection disc, and collecting optical signals;

or in the process of normal detection process, executing the step (3) in the emergency detection process.

Compared with the prior art, the invention has the beneficial effects that: through designing reagent card test tray, fixed optical detection module and having increased abandonment reagent card and having abandoned the motion subassembly, make reagent card detection efficiency improve greatly.

Drawings

The following further detailed description of embodiments of the invention is made with reference to the accompanying drawings:

FIG. 1 is a schematic diagram of the overall structure of a fully automatic fluorescence immunoassay analyzer according to the present invention;

FIG. 2 is a schematic view of the internal structure of the fully automatic fluoroimmunoassay analyzer of FIG. 1;

FIG. 3 is a schematic view of the internal structure of the fully automatic fluoroimmunoassay analyzer of FIG. 2 including a holder;

FIG. 4 is a schematic view of a sample inlet and outlet device partially shown in FIG. 2;

FIG. 5 is a schematic view of another perspective of the sample inlet and outlet device shown partially in FIG. 2;

FIG. 6 is a schematic view of the sample pushing hand of the sample feeding and discharging device shown in FIG. 4;

FIG. 7 is a schematic view of a sample introduction lateral pushing hand of the sample introduction and discharge device in FIG. 4;

FIG. 8 is a schematic diagram of the structure of the sample inlet and outlet platform of the sample inlet and outlet device partially shown in FIG. 4;

fig. 9 is a view of the structure of the test tube rack;

fig. 10 is a bottom view of the bottom of the test tube rack;

FIG. 11 is a schematic diagram of a sample tube mixing assembly of the present invention;

FIG. 12 is a schematic diagram of a portion of the sample tube homogenizing assembly of FIG. 11;

FIG. 13 is a schematic view of the sampling needle and diluent needle movement assembly of the present invention;

FIG. 14 is a schematic diagram of the reagent card magazine assembly, detection assembly and reagent card carrier assembly shown in part in FIG. 2;

FIG. 15 is a schematic view of a reagent cartridge assembly of the present invention;

FIG. 16 is a schematic view of a back side partial structure of the reagent cartridge assembly of the present invention;

FIG. 17 is a schematic view of the reagent card transfer mechanism of the reagent card magazine assembly, the detection assembly and the reagent card carrier assembly of FIG. 2 in a configuration in which they are positioned in the carrier track;

FIG. 18 is a partial enlarged structural view of the second carrying paddle and the reagent card transfer mechanism in FIG. 17;

FIG. 19 is an enlarged partial block diagram of the sensing assembly of FIG. 2;

FIG. 20 is an enlarged partial view of the reagent card test tray of FIG. 19;

FIG. 21 is a schematic diagram of the kinematic assembly of the reagent card test tray of FIG. 19;

FIG. 22 is a plan view of the internal structure of the fully automatic fluoroimmunoassay analyzer of the present invention;

fig. 23 is a partially enlarged structural view of fig. 22;

FIG. 24 is a schematic view of the configuration of a reagent card finger in the reagent card magazine assembly;

FIG. 25 is a schematic view of the reagent card finger of FIG. 24 in an initial position;

FIG. 26 is a structural diagram illustrating a movement state of the lateral pushing and poking hand;

FIG. 27 is a schematic view of a sample pushing handle of the sample feeding and discharging device in FIG. 4;

FIG. 28 is an enlarged fragmentary view of the reagent truck handle motion plate of FIG. 15;

FIG. 29 is an enlarged partial view of the first carrier paddle and the second carrier paddle of FIG. 17;

wherein: 1-support, 101-vertical plate, 2-base, 3-sample introduction and sample discharge device, 301-sample introduction push handle structure, 3011-sample introduction push handle, 3012-first slider component, 3013-first slide rail, 3014-first belt, 3015-first motor, 3016-first driving wheel, 3017-first driven wheel, 302-sample introduction horizontal push handle structure, 3021-horizontal push handle, 3022-second slider component, 3023-second slide rail, 3024-second belt, 3025-second motor, 3026-second driving wheel, 3027-second driven wheel, 303-sample discharge push handle structure, 3031-sample discharge push handle, 3032-third slider component, 3033-third slide rail, 3034-third belt, 3035-third motor, 3036-third driving wheel, 3037-third driven wheel, 304-sample introduction platform, 305-a transverse movement track, 306-a sample-out platform, 307-a test tube rack positioner mounting opening, 308-a sample tube transverse pushing hand outlet, 309-a sample-out pushing hand outlet, 4-a sample tube, 5-a sample tube mixing component, 501-a sample tube gripper jaw movement component, 5011-a gripper fixing piece, 50111-a gripper fixing piece I, 50112-a gripper fixing piece II, 5012-a gripper sliding groove, 5013-a gripper sliding block, 50131-a gripper sliding block I, 50132-a gripper sliding block II, 5014-a gripper connecting rod, 50141-a gripper connecting rod I, 50142-a gripper connecting rod II, 5015-a sample tube gripper motor shaft fixing block, 502-a sample tube swinging movement motor, 503-a sample tube swinging fixing block, 504-a sample tube gripper movement motor, 505-a sample tube gripper jaw, 5051-clamping jaw I, 5052-clamping jaw II and 506-sample tube upper and lower fixing blocks; 507-sample tube up-down slider assembly, 508-sample tube up-down movement slide rail, 509-sample tube up-down movement motor, 5010-sample tube up-down movement screw rod, 6-sampling needle and dilution needle movement assembly, 601-sampling needle, 602-sampling needle up-down movement assembly, 6021-sampling needle up-down movement motor, 6022-sampling needle up-down movement slider assembly, 6023-sampling needle up-down slide rail, 6024-sampling needle up-down movement motor screw rod, 603-sampling needle and dilution needle horizontal movement assembly, 6031-sampling needle and dilution needle horizontal movement motor, 6032-sampling needle and dilution needle horizontal movement slider assembly, 6033-sampling needle and dilution needle horizontal slide rail, 6034-sampling needle and dilution needle horizontal movement drive wheel, 6035-sampling needle and dilution needle horizontal movement belt, 6036-sampling needle and diluent needle horizontal movement driven wheel, 6037-sampling needle and diluent needle horizontal movement limit optical coupler, 604-fixed vertical plate, 605-guide bending belt, 606-sampling needle and diluent needle movement component support frame, 607-diluent needle, 608-diluent needle up-and-down movement component, 6081-diluent needle up-and-down movement motor, 6082-diluent needle up-and-down movement slide block component, 6083-diluent needle up-and-down slide rail, 609-diluent bin component, 6091-diluent bin, 6092-diluent bin movement motor, 6093-diluent bin slide block, 6094-diluent bin slide rail, 6095-diluent bin driving wheel, 6096-diluent bin driven wheel, 6097-diluent bin belt, 6010-diluent bottle, 6011-blending reaction tank, 7-reagent card bin component, 701-reagent cartridge, 7011-reagent card, 702-reagent card holder, 703-reagent card holder, 7031-reagent card holder partition, 704-reagent card hand-setting device, 7041-reagent card hand-setting motor, 7042-reagent card hand-setting driving wheel, 7043-reagent card hand-setting driven wheel, 7044-reagent card hand-setting belt, 7045-reagent card hand-setting connecting block, 7046-reagent card hand-setting slide rail, 7047-reagent card hand-setting, 7048-reagent card hand-setting head, 7049-reagent card hand-setting elastic member, 70410-reagent card hand-setting tail, 70411-reagent card hand-setting inclined portion, 705-bottom fixing block, 706-top fixing block, 707-top fixing block riser, 708-reagent card hand-setting motion block, 7081-reagent card hand-setting motion rail, 709-reagent card motion limit partition, 8-detection component, 801-reagent card detection disc, 8011-reagent card position, 8012-reagent card detection disc rotating motor, 8013-reagent card detection disc driving wheel, 8014-reagent card detection disc driven wheel, 8015-reagent card detection disc belt, 8016-reagent card clamping piece, 802-optical detection module, 8021-light collecting head, 8022-connecting arm, 8023-shielding case, 803-detection support, 804-support plate, 805-reagent card disposal hand-shifting device, 8051-reagent card disposal hand-shifting, 8052-reagent card disposal hand-shifting motor, 8053-reagent card disposal hand-shifting block component, 8054-reagent card disposal hand-shifting slide rail, 8055-reagent card disposal hand driving wheel, 8056-reagent card disposal hand-shifting driven wheel, 8057-reagent card disposal hand-shifting belt, 9-reagent card carrying assembly, 901-carrying track, 902-reagent card transferring mechanism, 9021-reagent card transferring bin, 9022-transferring motor, 903-carrying first hand shifting device, 9031-carrying first hand shifting motor, 9032-carrying first hand shifting driving wheel, 9033-carrying first hand shifting driven wheel, 9034-carrying first hand shifting belt, 9035-carrying first hand shifting, 9036-carrying first hand shifting block assembly, 9037-carrying first hand shifting rail and 904-carrying second hand shifting device; 9041-a second motor for carrying a second hand, 9042-a second driving wheel for carrying a second hand, 9043-a second driven wheel for carrying a second hand, 9044-a second belt for carrying a second hand, and 9045-a second hand; 9046-a carrying dialer two-slide block assembly, 9047-a carrying dialer two-slide rail, 10-an emergency sample placing position, 11-an emergency detection position, 12-a test tube rack, 1201-a test tube rack positioning groove, 1202-a bottom longitudinal partition block, 13-a reagent card code scanner, 14-a cleaning assembly, 1401-a cleaning liquid bottle, 1402-a cleaning liquid bottle support, 1403-a cleaning pool, 15-a shell, 16-a display screen, 17-a waste receiving barrel, 18-a cleaning liquid bottle storage inlet, 19-a built-in printer, 1901-a paper outlet, 20-an observation window, 21-an item code scanner, 22-a reagent cassette and diluent bottle storage inlet, and 23-a sample tube code scanner assembly.

Detailed Description

As shown in fig. 1 to 3 and 22, the full-automatic fluoroimmunoassay analyzer of the present embodiment includes a support 1, the support 1 is disposed on a base 2, a sample introduction and discharge device 3 is disposed on the base 2, and the sample introduction and discharge device 3 is configured to push a sample tube 4 into a sample introduction and push the sample tube 4 out of the sample introduction and discharge device for sample discharge; a sample tube blending assembly 5 is arranged on the side surface of the sample feeding and discharging device 3, the sample tube blending assembly 5 is used for blending samples in a sample tube 4, wherein the sample tube 4 is pushed in and out by the sample feeding and discharging device 3 to perform sample discharging; the device also comprises a sampling needle and dilution needle module 6, which is used for extracting a sample in the sample tube 4, adding the sample into the blending reaction tank 6011 (as shown in fig. 23), adding a diluent into the blending reaction tank 6011, diluting and blending, wherein the sampling needle and dilution needle module 6 comprises a sampling needle 601 and a dilution needle 607; as shown in fig. 15; a reagent card bin assembly 7 is arranged in the support 1, a plurality of reagent cassettes 701 are stacked in the reagent card bin assembly 7, and as shown in fig. 14, the sampling needle 601 adds the diluted and uniformly mixed sample to a sample adding port of the reagent card 7011; a reagent card carrying assembly is also arranged in the bracket and used for conveying a reagent card 7011; the support 1 is also internally provided with a detection component 8 for detecting the reagent card 7011.

The outer part of the bracket 1 is also covered with a shell 15, a display screen 16 is arranged on the shell 15, and the content related to detection can be viewed on the display screen 16; a waste receiving barrel 17, a cleaning solution bottle inlet 18, a reagent cassette and diluent bottle inlet 22 are arranged on the side surface of the shell 15; the full-automatic fluorescence immunoassay analyzer is also provided with a built-in printer 19, and a paper outlet 1901 of the built-in printer 19 is arranged on the shell 15; the shell 15 is also provided with an observation window 20 and an item code scanner 21;

as shown in fig. 4 to 8, the sample feeding and discharging device 3 includes a sample feeding pushing handle structure 301, a sample feeding transverse pushing and poking handle structure 302, a sample discharging pushing handle structure 303, and a transverse moving track 305 (as shown in fig. 8); the sample pushing handle structure 301 is used for pushing the sample tube 4 placed on the sample platform 304 of the sample inlet and outlet platform into the sample along the longitudinal direction; the sample feeding transverse pushing and poking hand structure 302 is used for pushing the sample tube 4 into a sample feeding along the transverse direction; the sample outlet pushing hand structure 303 is configured to push the detected sample tube 4 out to a sample outlet platform 306 in a sample outlet platform for sample outlet, as shown in fig. 8, the sample outlet platform includes a sample inlet platform 304 and a sample outlet platform 306, and a plurality of sample tube holder positioner mounting ports 307 are arranged on the side surface of the sample outlet platform; a sample tube transverse pushing and poking hand outlet 308 is arranged on the sample feeding platform; the side of the sample outlet platform 306 is provided with a sample outlet pusher 309.

Specifically, as shown in fig. 6, the sample push handle structure 301 has a sample push handle 3011, and the sample push handle 3011 is disposed on the first slide rail 3013 through the first slide block component 3012; the first slider component 3012 is fixed on the first belt 3014, the first motor 3015 is driven by the first driving wheel 3016, the first driven wheel 3017 and the first belt 3014, the first slider component 3012 moves longitudinally along the first slide rail 3013 in a reciprocating mode and is switched, and then the sample tube 4 (located on the test tube rack 12) is pushed into and sample introduction along the longitudinal direction.

The sample tube 4 (located on the sample tube rack 12) pushed by the sample pushing hand 3011 enters the transverse movement track 305, and the sample tube rack 12 performs sample injection and sample discharge along the transverse movement track 305, as shown in fig. 7, the sample transverse pushing hand structure 302 has a plurality of transverse shifting hands 3021, three in this embodiment, it should be noted that the structures of the sample transverse pushing hand and the mix reaction pool hand involved in this embodiment are the same, and include a shifting hand head, a shifting hand tail, and an inclined portion, when the transverse shifting hand 3021 transversely pushes the sample tube rack 12, the shifting hand head 7048 faces the pushing direction, so that when the transverse shifting hand 3021 moves back, the tube rack head 7048 moves back due to the effect of the bottom longitudinal partition block 1202 (shown in fig. 10) of the sample tube rack 12, and then moves forward by the effect of the transverse pushing hand motor, so that the shifting hand head 7048 can bounce to push the sample tube rack 12 to move again after pressing down, as shown in fig. 24, 26; the transverse shifting handle 3021 is arranged on the second sliding rail 3023 through the second sliding block assembly 3022; the second sliding block assembly 3022 is fixed on the second belt 3024, the second motor 3025 drives the second sliding block assembly 3022 to transversely reciprocate along the second sliding rail 3023 through the second driving wheel 3026, the second driven wheel 3027 and the second belt 3024, so that the sample tube 4 on the sample tube rack 12 is transversely pushed into the sample injection port; the sample tubes 4 are placed on a sample tube rack 12 having a plurality of sample tube rack positioning slots 1201, as shown in fig. 9, the sample tube rack 12 illustrating 10 sample tube positions, a preferred number; the distance between two adjacent lateral picks 3021 is no greater than the length of the sample tube rack 12.

As shown in fig. 27, the sample ejection push handle structure 303 has a sample ejection push handle 3031, and the sample ejection push handle 3031 is arranged on a sliding rail 3033 through a sliding block assembly 3032; the sliding block assembly three 3032 is fixed on the belt three 3034, the motor three 3035 drives the sliding block assembly three 3032 to longitudinally reciprocate along the sliding rail three 3033 through the driving wheel three 3036, the driven wheel three 3037 and the belt three 3034, so as to push the detected sample tube 4 out of the sample outlet platform 306 for sample outlet, as shown in fig. 8.

As shown in fig. 11, the sample tube blending assembly 5 includes a sample tube up-down movement slider assembly, a sample tube up-down movement motor 509, a sample tube up-down movement slide rail 508, a sample tube gripper movement assembly 501, a sample tube gripper movement motor 504, a sample tube swinging movement motor 502, and a sample tube swinging fixing block 503, wherein the sample tube gripper movement motor 504 is connected with the sample tube gripper movement assembly 501, and the sample tube gripper movement assembly 501 is connected with a sample tube gripper 505; the sample tube clamping jaw moving assembly 501 is connected with the sample tube swinging fixing block 503 and the sample tube up-and-down moving slide block assembly, and the sample tube swinging motor 502 and the sample tube up-and-down moving slide block assembly are connected with the sample tube swinging fixing block 503; the sample tube up-down movement motor 509 and the sample tube up-down movement slide rail 508 are both connected to the sample tube up-down movement slide assembly. The sample tube 4 is clamped by the sample tube clamping jaw 505, the sample tube up-and-down movement motor 509 drives the sample tube up-and-down movement slider assembly connected with the sample tube clamping jaw movement assembly 501 to move along the sample tube up-and-down movement slide rail 508, so that the sample tube clamping jaw 505 connected with the sample tube up-and-down movement slider assembly moves along the sample tube up-and-down movement slide rail 508, and the sample tube clamping jaw 505 is driven to clamp the sample tube 4 to move up and down; the sample tube swinging motor 502 drives the sample tube swinging fixed block 503 to move, and drives the sample tube clamping jaw moving component 501 connected with the sample tube swinging fixed block 503 to swing, so as to drive the sample tube clamping jaw 505 to clamp the sample tube 4 for swinging and uniform mixing; the sample tube jaw movement motor 504 drives the sample tube jaw movement assembly 501 to control the sample tube jaw 505 to clamp and unclamp the sample tube 4; the sample tube up-and-down movement motor 509 is a screw motor; the side surface of the sample tube up-and-down movement motor 509 is provided with an up-and-down movement optical coupler, and correspondingly, the sample tube up-and-down movement sliding block component is provided with an up-and-down movement sensing chip; the sample tube jaw movement motor 504 and the sample tube swing movement motor 502 are both stepper motors; the sample tube up-and-down movement slider assembly comprises a sample tube up-and-down slider assembly 507 and a sample tube up-and-down fixing block 506, the sample tube up-and-down slider assembly 507 is connected with the sample tube up-and-down movement slide rail 508 and moves up and down along the sample tube up-and-down movement slide rail 508, and the sample tube up-and-down fixing block 506 is connected with the sample tube up-and-down movement motor 509 through a sample tube up-and-down movement lead screw 5010.

As shown in fig. 12, the sample tube jaw movement assembly 501 includes a jaw fixing plate 5011, a jaw sliding slot 5012, a jaw sliding block 5013, a jaw connecting rod 5014, and a sample tube jaw motor shaft fixing block 5015, the sample tube jaw motor shaft fixing block 5015 is connected to the sample tube jaw movement motor 504, the sample tube jaw 505 is connected to the jaw fixing plate 5011, the jaw fixing plate 5011 is connected to the sample tube jaw motor shaft fixing block 5015 through the jaw connecting rod 5014, and the jaw fixing plate 5011 is movably connected to the jaw connecting rod 5014; the jaw slide 5012 is connected to the sample tube clamp jaw motor shaft fixing block 5015, and the jaw fixing piece 5011 is connected to the jaw slide 5012 through the jaw slider 5013 and moves horizontally in the jaw slide 5012, so that the sample tube 4 is clamped and released by the sample tube clamp jaw 505.

As shown in fig. 12, the sample tube jaw 505 includes a first jaw 5051 and a second jaw 5052, and correspondingly, the jaw link 5014 includes a first jaw link 50141 and a second jaw link 50142, the jaw fixing piece 5011 includes a first jaw fixing piece 50111 and a second jaw fixing piece 50112, the jaw slider 5013 includes a first jaw slider 50131 and a second jaw slider 50132, one side of the first jaw 5051 is connected to the first jaw link 50141 through the first jaw fixing piece 50111, and the first jaw fixing piece 50111 is fixedly connected to the first jaw slider 50131; one side of the second clamping jaw 5052 is connected with the second clamping jaw connecting rod 50142 through a second clamping jaw fixing sheet 50112, and the second clamping jaw fixing sheet 50112 is fixedly connected to the second clamping jaw sliding block 50132; the first jaw slide 50131 and the second jaw slide 50132 are both connected to the jaw slot 5012 and move horizontally in the jaw slot 5012 to move the first jaw 5051 and the second jaw 5052 horizontally inward or outward at the same time to clamp and unclamp the sample tube.

As shown in fig. 13, the sampling needle and dilution needle module comprises a dilution needle up-and-down movement assembly 608, a sampling needle up-and-down movement assembly 602, and a sampling needle and dilution needle horizontal movement assembly 603;

the diluent needle up-and-down movement assembly 608 comprises a diluent needle up-and-down movement motor 6081 and a diluent needle up-and-down movement slider assembly 6082, the diluent needle 607 is arranged on the diluent needle up-and-down movement slider assembly 6082, and the diluent needle up-and-down movement motor 6081 drives the diluent needle up-and-down movement slider assembly 6082 to reciprocate along a diluent needle up-and-down sliding rail 6083;

the sampling needle up-and-down movement assembly 602 comprises a sampling needle up-and-down movement motor 6021 and a sampling needle up-and-down movement slide block assembly 6022, the sampling needle 601 is arranged on the sampling needle up-and-down movement slide block assembly 6022, and the sampling needle up-and-down movement motor 6021 drives the sampling needle up-and-down movement slide block assembly 6022 to reciprocate along a sampling needle up-and-down sliding rail 6023;

the sampling needle and dilution needle horizontal movement assembly 603 comprises a sampling needle and dilution needle horizontal movement motor 6031 and a sampling needle and dilution needle horizontal movement sliding block assembly 6032, the sampling needle up-and-down movement assembly 602 and the dilution needle up-and-down movement assembly 608 are all installed on a fixed vertical plate 604 of the sampling needle and dilution needle horizontal movement sliding block assembly 6032, the sampling needle and dilution needle horizontal movement motor 6031 drives the sampling needle and dilution needle horizontal movement sliding block assembly 6032 to reciprocate on a sampling needle and dilution needle horizontal sliding rail 6033.

In this embodiment, the sampling needle up-and-down movement motor 6021 and the dilution needle up-and-down movement motor 6081 are both screw rod motors, and the sampling needle up-and-down movement slide block assembly 6022 is sleeved on a sampling needle up-and-down movement motor screw rod 6024 connected to the sampling needle up-and-down movement motor 6021; the diluent needle up-and-down movement sliding block assembly 6082 is sleeved on a diluent needle up-and-down movement motor screw rod connected with the diluent needle up-and-down movement motor 6081; the sampling needle is step motor with dilution needle horizontal movement motor 6031, the sampling needle is connected with dilution needle horizontal movement action wheel 6034 with the sampling needle with dilution needle horizontal movement motor 6031, the sampling needle is connected with dilution needle horizontal movement action wheel 6036 with sampling needle and dilution needle horizontal movement from the driving wheel 6035 through sampling needle and dilution needle horizontal movement belt 6035 with the sampling needle, the sampling needle cup joints with dilution needle horizontal movement sliding block assembly 6032 on the sampling needle and dilution needle horizontal movement belt 6035, just the sampling needle with dilution needle horizontal movement sliding block assembly 6032 with the sampling needle with dilution needle horizontal sliding rail 6033 sliding connection. The sampling needle and dilution needle horizontal movement motor 6031 drives the sampling needle and dilution needle horizontal movement driving wheel 6034 to rotate, and the sampling needle and dilution needle horizontal movement driven wheel 6036 is driven to rotate by a sampling needle and dilution needle horizontal movement belt 6035 sleeved on the sampling needle and dilution needle horizontal movement driving wheel 6034, so that the sampling needle and dilution needle horizontal movement sliding block assembly 6032 horizontally moves along a sampling needle and dilution needle horizontal movement sliding rail 6033; the fixed riser 604 is connected to the sampling needle and diluent needle horizontal movement belt 6035 through the sampling needle and diluent needle horizontal movement slider assembly 6032.

The sampling needle and dilution needle movement assembly 6 further comprises a guide bending belt 605, one end of the guide bending belt 605 is fixed above the fixed vertical plate 604, the other end of the guide bending belt 605 is arranged on a support frame 606 of the sampling needle and dilution needle movement assembly, and the guide bending belt 605 is longitudinally arranged along a horizontal slide rail 6033 of the sampling needle and the dilution needle.

A sampling needle up-and-down movement limiting optocoupler is arranged on the fixed vertical plate 604 and at the upper end of the sampling needle up-and-down sliding rail 6023; on sampling needle and the dilution needle motion subassembly support frame, and be located and be close to sampling needle and dilution needle horizontal guide's end afterbody is provided with the spacing opto-coupler 6037 of sampling needle and dilution needle horizontal motion.

The sampling needle and diluent needle module further comprises a diluent bin assembly 609, the diluent bin assembly 609 comprises a diluent bin 6091, a diluent bin moving motor 6092, a diluent bin sliding block 6093, a diluent bin sliding rail 6094 and a diluent bin moving assembly, the diluent bin moving motor 6092 is connected with the diluent bin moving assembly, and the diluent bin sliding block 6093 is respectively connected with the diluent bin 6091 and the diluent bin sliding rail 6094, so that the diluent bin moving motor 6092 drives the diluent bin 6091 to reciprocate along the diluent bin sliding rail 6094; the diluent bin 6091 is positioned below the diluent needle 607 and is used for placing a plurality of diluent bottles 6010; and a blending reaction tank 6011 is arranged on the side surface of the diluent bin 6091. The dilution needle 607 can obtain the dilution solution from the dilution solution bottle 6010 through a pipeline and inject the dilution solution into the homogenizing reaction tank 6012.

Diluent storehouse motion subassembly includes diluent storehouse action wheel 6095, diluent storehouse from driving wheel 6096 and diluent storehouse belt 6097, diluent storehouse motion motor 6092 is step motor, diluent storehouse motion motor 6092 with diluent storehouse action wheel 6095 is connected, diluent storehouse belt 6097 cup joints diluent storehouse action wheel 6095 and diluent storehouse are from driving wheel 6096 on, diluent storehouse slider 6093 cup joints on the diluent storehouse belt 6097.

As shown in fig. 14 to 16, the reagent cartridge bay assembly 7 includes a reagent cartridge bay 703, and a plurality of reagent cartridge bay partitions 7031 are disposed in the reagent cartridge bay 703, so that the reagent cartridge bay 703 has a plurality of mutually independent reagent cartridge bay slots for placing the reagent cartridge 701; the reagent card bin partition 7031 is fixed by a bottom fixing block 705 and a top fixing block 706; the reagent card chamber partition 7031 is arc-shaped, the reagent card box 701 is placed in the reagent card chamber partition 7031, the top and the bottom of the reagent card chamber partition are fixed and limited by the top fixing block 706 and the bottom fixing block 705 respectively, and the reagent card chamber partition is stable in structure.

The inner side wall of the reagent card bin 703 is provided with reagent card detectors for detecting the number of reagent cards; the reagent card detector is arranged on the side wall of the reagent card bin 703, can detect the number of the reagent cards 7011 and remind users to add the reagent cards 7011 in time; 20-30 reagent cards can be placed in one reagent cassette 701, and can be placed according to detection items and configured according to the required quantity.

The reagent card bin assembly 7 further comprises a plurality of reagent card hand-dialing devices 704, the reagent card hand-dialing devices 704 are provided with reagent card hand-dialing motors 7041, and the reagent card hand-dialing motors 7041 drive reagent card hand-dialing connecting blocks 7045 fixedly connected with the reagent card hand-dialing belts 7044 to reciprocate along reagent card hand-dialing sliding rails 7046 through reagent card hand-dialing driving wheels 7042, reagent card hand-dialing driven wheels 7043 and reagent card hand-dialing belts 7044; a reagent card shifting handle 7047 is arranged on the reagent card shifting handle connecting block 7045; the reagent card bin 703 is arranged above the reagent card hand-shifting device 704 through a reagent card bin bracket 702; as shown in fig. 28, the reagent card hand-dialing device 704 further includes a reagent card hand-dialing motion plate 708, the reagent card hand-dialing motion plate 708 is located at the bottom of the reagent card compartment 703, a reagent card hand-dialing motion track 7081 corresponding to the number of the reagent cassette slots is arranged on the reagent card hand-dialing motion plate 708 in a penetrating manner, and the reagent card hand-dialing 7047 dials the reagent cards 7011 in the reagent cassette 701 along the reagent card hand-dialing motion track 7081 to the carrying track 901 through the reagent card hand-dialing motion track 7081; the reagent card hand-pulling movement track 7081 is provided with a reagent card movement limiting partition 709, and the reagent card movement limiting partition 709 is positioned below a vertical plate 707 of a top fixing block of the reagent card cabin 703 and is used for ensuring a linear track for reagent card movement and enabling reagent card carrying to be carried smoothly. The number of reagent card casket groove in this embodiment is 5, and reagent card thumb wheel device is 5, and correspondingly, the orbital quantity of reagent card thumb wheel motion on the reagent card thumb wheel motion plate is 5.

The reagent card shifting handle 7047 comprises a reagent card shifting handle head 7048, as shown in fig. 24 and 25, a reagent card shifting handle elastic part 7049 is arranged below the reagent card shifting handle head 7048, and a common spring is adopted in the embodiment; the reagent card shifting handle 7047 is further provided with a reagent card shifting handle tail 70410 movably connected with the reagent card shifting handle connecting block 7045, and the reagent card shifting handle head 7048 is connected with the reagent card shifting handle tail 70410 through a reagent card shifting handle inclined part 70411; an upper pressure plate is arranged on the reagent cartridge holder 702; the reagent card hand 7047 pushes the reagent card 7011 out and then moves backward, the reagent card hand 7048 is pushed down due to the pressing of the reagent card 7011, the reagent card hand 7047 is at the initial position in fig. 25 when being reset, the reagent card cabin 703 is not affected to be pushed and pulled, the reagent card hand 7047 moves forward when needing to work, and the reagent card hand 7048 is popped out to push the reagent card 7011 to move when moving out of the upper pressing plate 7021.

As shown in fig. 17, the reagent card carrying assembly 9 includes a reagent card transfer mechanism 902 and a carrying track 901, wherein a reagent card 7011 in a reagent cassette 701 in the reagent card compartment 703 is firstly moved to the carrying track 901 by a reagent card moving hand 7047, then moved to the reagent card transfer mechanism 902 by a first carrying moving hand 903, and then moved to the detection assembly 8 of the reagent card by a second carrying moving hand 904; after the reagent card hand-dialing 7047 moves to the reagent card carrying track 901, it moves backward to the initial position, and under the action of the spring (reagent card hand-dialing elastic part 7049) and the upper pressing plate, the reagent card hand-dialing head 7048 is pressed down, and when the reagent card hand-dialing 7047 continues to dial the reagent card to the carrying track 901, the reagent card hand-dialing head 7048 bounces to push the reagent card 7011 to the reagent card carrying track 901.

As shown in fig. 17 to 18, the reagent card transferring mechanism 902 includes a transferring motor 9022 and a reagent card transferring chamber 9021, the reagent card transferring chamber 9021 is connected to the transferring motor 9022, and the transferring motor 9022 drives the reagent card transferring chamber 9021 to rotate horizontally; as shown in fig. 29, the first carrying lever device 903 includes a first carrying lever 9035, a first motor 9031 for carrying lever, a first moving component for carrying lever, a first sliding block component 9036 for carrying lever, and a first sliding rail 9037 for carrying lever, the first carrying lever 9035 is connected to the first sliding block component 9036 for carrying lever, the first sliding block component 9036 for carrying lever is sleeved on the first sliding rail 9037 for carrying lever and the first moving component for carrying lever, the first motor 9031 for carrying lever is connected to the first moving component for carrying lever, the first motor 9031 for carrying lever drives the first moving component for carrying lever to drive the first sliding block component 9036 for carrying lever to move, so as to drive the first carrying lever 9035 connected to the first sliding block component 9036 for carrying lever to reciprocate along the first sliding rail 9037 for carrying lever; the second carrying and shifting device 904 comprises a second carrying and shifting hand 9045, a second carrying and shifting hand motor 9041, a second carrying and shifting hand moving component, a second carrying and shifting hand sliding block component 9046 and a second carrying and shifting hand sliding rail 9047, the second carrying and shifting hand 9045 is connected with the second carrying and shifting hand sliding block component 9046, the second carrying and shifting hand sliding block component 9046 is sleeved on the second carrying and shifting hand sliding rail 9047 and the second carrying and shifting hand moving component, the second carrying and shifting hand motor 9041 is connected with the second carrying and shifting hand moving component, the second carrying and shifting hand moving component is driven by the second carrying and shifting hand motor 9042 to drive the second carrying and shifting hand sliding block component 9046 to move, and therefore the second carrying and shifting hand 9045 connected with the second carrying and shifting hand sliding block component 9046 is driven to reciprocate along the second carrying and shifting hand sliding rail 9047. The first carrying shifting handle movement component comprises a first carrying shifting handle driving wheel 9032, a first carrying shifting handle driven wheel 9033 and a first carrying shifting handle belt 9034, the first carrying shifting handle belt 9034 is sleeved between the first carrying shifting handle driving wheel 9032 and the first carrying shifting handle driven wheel 9033, the first carrying shifting handle driving wheel 9032 is connected with a first carrying shifting handle motor 9031, and the first carrying shifting handle slider component 9036 is sleeved on the first carrying shifting handle belt 9034; the second carrying shifting hand movement component comprises a second carrying shifting hand driving wheel 9042, a second carrying shifting hand driven wheel 9043 and a second carrying shifting hand belt 9044, the second carrying shifting hand belt 9044 is sleeved between the second carrying shifting hand driving wheel 9042 and the second carrying shifting hand driven wheel 9043, the second carrying shifting hand driving wheel 9042 is connected with a second carrying shifting hand motor 9041, and the second carrying shifting hand slider component 9046 is sleeved on the second carrying shifting hand belt 9044.

The second carrying shifting hand 9045 and the reagent card shifting hand 7047 have the same structure and comprise shifting hand heads, and elastic pieces are arranged below the shifting hand heads; the reagent card shifting hand is also provided with a shifting hand tail part, the shifting hand tail part is movably connected with a shifting hand sliding block component (a reagent card shifting hand connecting block and a carrying shifting hand two-sliding block component), and the shifting hand head part is connected with the shifting hand tail part through an inclined part.

As shown in fig. 19 to 21, the detecting assembly 8 includes a reagent card detecting tray 801 and an optical detecting module 802, the optical detecting module 802 is mounted on a supporting plate 804 through a detecting bracket 803, and a light collecting head 8021 of the optical detecting module 802 is connected to the detecting bracket 803 through a connecting arm 8022, so that the light collecting head 8021 is disposed above the reagent card detecting tray 801 and corresponds to a position of a reagent card detecting window on the reagent card detecting tray 801, thereby realizing that the light collecting head 8021 collects optical signals for a reagent card detecting window located below the light collecting head 8021; and a shielding cover 8023 is also arranged at the top of the lighting head 8021. The bottom of the reagent card detection tray 801 is provided with a constant temperature heating module for heating the reagent card 7011 and keeping the reagent card 7011 at a constant temperature.

As shown in fig. 21, a reagent card detection tray rotary motor 8012 is connected to the reagent card detection tray 801 for driving the reagent card detection tray 801 to rotate; a plurality of reagent screens 8011 are uniformly distributed on the reagent card detection tray 801 and are used for placing reagent cards 7011. The reagent card detection tray rotating motor 8012 is connected with the reagent card detection tray driving wheel 8013, the reagent card detection tray 801 is connected with the reagent card detection tray driven wheel 8014, and the reagent card detection tray belt 8015 is sleeved between the reagent card detection tray driving wheel 8013 and the reagent card detection tray driven wheel 8014, so that the reagent card detection tray 801 is driven to rotate by the reagent card detection tray rotating motor 8012.

As shown in fig. 19, the reagent card discarding hand-shifting device 805 is disposed at a position corresponding to the lighting head 8021 and outside the reagent card detecting tray 801, the reagent card discarding hand-shifting device 805 includes a reagent card discarding hand-shifting 8051, a reagent card discarding hand-shifting motor 8052, a reagent card discarding hand-shifting slider component 8053, a reagent card discarding hand-shifting slide rail 8054 and a reagent card discarding hand-shifting moving component, the reagent card discarding hand-shifting 8051 is connected to the reagent card discarding hand-shifting slider component 8053, the reagent card discarding hand-shifting motor 8052 is connected to the reagent card discarding hand-shifting moving component, the reagent card discarding hand-shifting slider component 8053 is sleeved on the reagent card discarding hand-shifting component and the reagent card discarding hand-shifting slide rail 8054, the reagent card discarding hand-shifting motor 8052 drives the reagent card discarding hand-shifting moving component to drive the reagent card discarding hand-shifting slider component 8053 to move, thereby driving the reagent card disposal hand lever 8051 connected to the reagent card disposal hand lever slider assembly 8053 to reciprocate along the reagent card disposal hand lever slide rail 8054; by the arrangement, the reagent card 7011 after detection is finished is moved to the waste receiving barrel 17 by the abandoned moving hand 8051, so that the detection efficiency of the reagent card 7011 is greatly improved; the reagent card disposal hand-shifting motion assembly comprises a reagent card disposal hand-shifting driving wheel 8055, a reagent card disposal hand-shifting driven wheel 8056 and a reagent card disposal hand-shifting belt 8057, wherein the reagent card disposal hand-shifting belt 8057 is sleeved between the reagent card disposal hand-shifting driving wheel 8055 and the reagent card disposal hand-shifting driven wheel 8056, the reagent card disposal hand-shifting driving wheel 8055 is connected with the reagent card disposal hand-shifting motor 8052, and the reagent card disposal hand-shifting block assembly 8053 is sleeved on the reagent card disposal hand-shifting belt 8057.

As shown in fig. 20, both sides of the reagent card position 8011 are provided with reagent card clamping pieces 8016 for clamping both sides of the reagent card 7011, so that the reagent card 7011 is fixed in the reagent card detection tray 801.

A reagent card code scanner 13 (shown in fig. 16) is arranged in the bracket 1, and the reagent card code scanner 13 is arranged above the reagent card transfer bin; a cleaning assembly 14 is arranged above the first carrying shifting handle, and a plurality of cleaning liquid bottles 1401 are placed on the cleaning assembly through a cleaning liquid bottle support 1402; when the sampling needle cleaning assembly 14 is used for cleaning the sampling needle 601, the inner wall of the needle enters the sampling needle 601 from a cleaning system to be washed, the outer wall of the needle enters cleaning liquid from a side inlet to be washed, and waste liquid is discharged from a lower discharge port through a liquid discharge system. The full-automatic fluorescence immunoassay analyzer of the embodiment further comprises an emergency sample placing position, wherein the emergency sample placing position 10 is used for placing the sample tube 4, as shown in fig. 4-5; and is provided with an emergency treatment detection position 11 and an emergency treatment sample tube detection sensor; the emergency sample placement site 10 is located in the horizontal movement direction of the sampling needle 601. A cleaning pool 1403 is arranged on one side of the emergency detection position 11 and used for cleaning the sampling needle 601; and a sample tube code scanner assembly 23 is arranged between the sample introduction platform 304 and the emergency detection position 11 and is used for scanning the information of the sample tube for sample introduction.

The emergency detection process comprises the following steps:

(2) after the project is imported, a reagent card is put in, a diluent bottle is put in, and the patient information, the sample information and the project to be detected are recorded;

(3) opening an emergency sample placing position, inserting an emergency sample tube into an emergency sample detection position, and starting a detection process after detecting that the emergency sample is inserted; the reagent card is pulled out from the reagent card bin and carried to a reagent card detection plate, a sampling needle dilution needle module is started to sample the sample to a reagent card sample adding port, a heating module is started to keep the reagent card in a set temperature for incubation; after the incubation reaction is finished, rotating the reagent card to be detected to the lower part of a lighting head of the optical detection module through the rotation of the reagent card detection disc, and collecting optical signals;

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. A full-automatic fluorescence immunoassay analyzer comprises a bracket, wherein the bracket is arranged on a base, and is characterized in that a sample feeding device and a sample discharging device are arranged on the base;

a sample tube blending assembly is arranged on the side surface of the sample feeding and discharging device and used for blending samples in a sample tube, wherein the sample tube is pushed in and out by the sample feeding and discharging device to perform sample discharging;

the device also comprises a sampling needle and dilution needle module which is used for extracting a sample in a sample tube, adding the sample into a mixing reaction tank, adding a diluent into the mixing reaction tank for dilution and mixing, wherein the sampling needle and dilution needle module comprises a sampling needle and a dilution needle; the sampling needle adds the diluted and uniformly mixed sample into a sample adding port of the reagent card;

2. The full-automatic fluoroimmunoassay analyzer according to claim 1, wherein the sample tube mixing assembly comprises a sample tube up-and-down movement slider assembly, a sample tube up-and-down movement motor, a sample tube up-and-down movement slide rail, a sample tube gripper movement assembly, a sample tube gripper movement motor, a sample tube swinging movement motor and a sample tube swinging fixing block, the sample tube gripper movement motor is connected with the sample tube gripper movement assembly, and the sample tube gripper movement assembly is connected with the sample tube gripper; the sample tube clamping jaw moving assembly is connected with the sample tube swinging fixing block and the sample tube up-and-down moving sliding block assembly, and the sample tube swinging moving motor and the sample tube up-and-down moving sliding block assembly are connected with the sample tube swinging fixing block; the sample tube up-and-down movement motor and the sample tube up-and-down movement sliding rail are connected with the sample tube up-and-down movement sliding block component.

3. The automatic fluoroimmunoassay analyzer of claim 2, wherein the sample tube up-and-down moving slider assembly comprises a sample tube up-and-down slider assembly and a sample tube up-and-down fixing block, the sample tube up-and-down slider assembly is connected with the sample tube up-and-down moving slide rail and moves up and down along the sample tube up-and-down moving slide rail, and the sample tube up-and-down fixing block is connected with the sample tube up-and-down moving motor through a sample tube up-and-down moving screw rod.

4. The fully automatic fluoroimmunoassay analyzer of claim 2, wherein the sample tube jaw movement assembly comprises a jaw fixing plate, a jaw chute, a jaw slider, a jaw connecting rod and a sample tube jaw motor shaft fixing block, the sample tube jaw motor shaft fixing block is connected with the sample tube jaw movement motor, the sample tube jaw is connected with the jaw fixing plate, the jaw fixing plate is connected with the sample tube jaw motor shaft fixing block through the jaw connecting rod, and the jaw fixing plate is movably connected with the jaw connecting rod; the clamping jaw sliding groove is connected with a sample tube clamping jaw motor shaft fixing block, and the clamping jaw fixing block is connected with the clamping jaw sliding groove through the clamping jaw sliding block and moves horizontally in the clamping jaw sliding groove, so that a sample tube is clamped and loosened by a sample tube clamping jaw.

5. The full-automatic fluoroimmunoassay analyzer according to claim 4, wherein the sample tube clamping jaw comprises a first clamping jaw and a second clamping jaw, correspondingly, the clamping jaw connecting rod comprises a first clamping jaw connecting rod and a second clamping jaw connecting rod, the clamping jaw fixing plate comprises a first clamping jaw fixing plate and a second clamping jaw fixing plate, the clamping jaw sliding block comprises a first clamping jaw sliding block and a second clamping jaw sliding block, one side of the first clamping jaw is connected with the first clamping jaw connecting rod through the first clamping jaw fixing plate, and the first clamping jaw fixing plate is fixedly connected with the first clamping jaw sliding block; one side of the clamping jaw II is connected with the clamping jaw connecting rod II through the clamping jaw fixing piece II, and the clamping jaw fixing piece II is fixedly connected to the clamping jaw sliding block II; the first clamping jaw sliding block and the second clamping jaw sliding block are connected with the clamping jaw sliding groove and move horizontally in the clamping jaw sliding groove, so that the first clamping jaw and the second clamping jaw simultaneously move inwards or outwards horizontally, and a sample tube is clamped and loosened.

6. The fully automatic fluoroimmunoassay analyzer of claim 1, wherein the sampling needle and dilution needle module comprises a dilution needle up-and-down movement assembly, a sampling needle up-and-down movement assembly and a sampling needle and dilution needle horizontal movement assembly;

7. The full-automatic fluoroimmunoassay analyzer according to claim 6, wherein the sampling needle and diluent needle module further comprises a diluent bin assembly, the diluent bin assembly comprises a diluent bin, a diluent bin moving motor, a diluent bin slider, a diluent bin slide rail and a diluent bin moving assembly, the diluent bin moving motor is connected with the diluent bin moving assembly, and the diluent bin slider is respectively connected with the diluent bin and the diluent bin slide rail, so that the diluent bin moving motor drives the diluent bin to reciprocate along the diluent bin slide rail; the diluent bin is positioned below the diluent needle and is used for placing a plurality of diluent bottles; and a blending reaction tank is arranged on the side surface of the diluent bin.

8. The full-automatic fluoroimmunoassay analyzer of claim 6, wherein the sampling needle up-and-down movement motor and the dilution needle up-and-down movement motor are both screw rod motors, and the sampling needle up-and-down movement slider assembly is sleeved on a sampling needle up-and-down movement motor screw rod connected with the sampling needle up-and-down movement motor; the diluent needle up-and-down movement sliding block assembly is sleeved on a diluent needle up-and-down movement motor screw rod connected with the diluent needle up-and-down movement motor; the sampling needle is step motor with dilution needle horizontal motion motor, the sampling needle is connected with sampling needle and dilution needle horizontal motion action wheel with dilution needle horizontal motion motor, sampling needle and dilution needle horizontal motion action wheel are connected with sampling needle and dilution needle horizontal motion from the driving wheel through sampling needle and dilution needle horizontal motion belt, sampling needle and dilution needle horizontal motion sliding block assembly cup joint on sampling needle and the dilution needle horizontal motion belt, just sampling needle and dilution needle horizontal sliding block assembly with sampling needle and dilution needle horizontal slide rail sliding connection.

9. The automatic fluoroimmunoassay analyzer of claim 7, wherein the diluent bin moving assembly comprises a diluent bin driving wheel, a diluent bin driven wheel and a diluent bin belt, the diluent bin moving motor is a stepping motor, the diluent bin moving motor is connected with the diluent bin driving wheel, the diluent bin belt is sleeved on the diluent bin driving wheel and the diluent bin driven wheel, and the diluent bin sliding block is sleeved on the diluent bin belt.

10. The full-automatic fluoroimmunoassay analyzer of claim 1, wherein the reagent cartridge compartment assembly comprises a reagent cartridge compartment, wherein a plurality of reagent cartridge compartment partitions are disposed in the reagent cartridge compartment to make the reagent cartridge compartment have a plurality of independent reagent cartridge slots for accommodating the reagent cartridges; the reagent card bin partition is fixed through the bottom fixing block and the top fixing block.

11. The full-automatic fluoroimmunoassay analyzer of claim 10, wherein the reagent cartridge magazine assembly further comprises a plurality of reagent cartridge paddle devices having reagent cartridge paddle motors that drive reagent cartridge paddle connecting blocks fixedly connected to the reagent cartridge paddle belts to reciprocate along reagent cartridge paddle slide rails via reagent cartridge paddle driving wheels, reagent cartridge paddle driven wheels, and reagent cartridge paddle belts; a reagent card shifting hand is arranged on the reagent card shifting hand connecting block; the reagent card hand-dialing device also comprises a reagent card hand-dialing motion plate, the reagent card hand-dialing motion plate is positioned at the bottom of the reagent card bin, reagent card hand-dialing motion tracks with the number corresponding to that of the reagent card cassette slots penetrate through the reagent card hand-dialing motion plate, and the reagent card hand-dialing passes through the reagent card hand-dialing motion tracks to dial reagent cards in the reagent card cassettes to move to the reagent card carrying assembly along the reagent card hand-dialing motion tracks; reagent card motion is set up the spacing wall of reagent card motion on the reagent card thumb wheel motion track, reagent card motion is spacing to be located the below of the top fixed block riser in reagent card storehouse.

12. The automated fluoroimmunoassay analyzer of claim 11, wherein the reagent card carrying assembly comprises a reagent card transferring mechanism and a carrying track, wherein the reagent card in the reagent cassette in the reagent card compartment is firstly moved to the carrying track by the reagent card moving hand, then moved to the reagent card transferring mechanism by the first carrying moving hand device, and then moved to the detection assembly of the reagent card by the second carrying moving hand device.

13. The automatic fluoroimmunoassay analyzer of claim 12, wherein the reagent card transferring mechanism comprises a transferring motor and a reagent card transferring chamber, the reagent card transferring chamber is connected with the transferring motor, and the reagent card transferring chamber is driven by the transferring motor to rotate horizontally; the carrying shifting hand device comprises a carrying shifting hand I, a carrying shifting hand I motor, a carrying shifting hand I motion assembly, a carrying shifting hand I slide block assembly and a carrying shifting hand I slide rail, wherein the carrying shifting hand I is connected with the carrying shifting hand I slide block assembly, the carrying shifting hand I slide block assembly is sleeved on the carrying shifting hand I slide rail and the carrying shifting hand I motion assembly, the carrying shifting hand I motor is connected with the carrying shifting hand I motion assembly, and the carrying shifting hand I motion assembly is driven by the carrying shifting hand I motor to drive the carrying shifting hand I slide block assembly to move so as to drive the carrying shifting hand I connected with the carrying shifting hand I slide block assembly to reciprocate along the carrying shifting hand I slide rail; the second carrying shifting hand device comprises a second carrying shifting hand, a second motor for the carrying shifting hand, a second moving assembly for the carrying shifting hand, a second sliding block assembly for the carrying shifting hand and a second sliding rail for the carrying shifting hand, the second carrying shifting hand is connected with the second sliding block assembly for the carrying shifting hand, the second sliding block assembly for the carrying shifting hand is sleeved on the second sliding rail for the carrying shifting hand and the second moving assembly for the carrying shifting hand, the second motor for the carrying shifting hand is connected with the second moving assembly for the carrying shifting hand, and the second moving assembly for the carrying shifting hand is driven by the second motor for the carrying shifting hand to drive the second carrying shifting hand connected with the second sliding block assembly for the carrying shifting hand to reciprocate along the second sliding rail for the carrying shifting hand.

14. The full-automatic fluoroimmunoassay analyzer according to claim 1, wherein the detecting assembly comprises a reagent card detecting tray and an optical detecting module, the optical detecting module is mounted on a supporting plate through a detecting bracket, and a light collecting head of the optical detecting module is connected with the detecting bracket through a connecting arm, so that the light collecting head is arranged above the reagent card detecting tray and corresponds to the position of a reagent card detecting window on the reagent card detecting tray, thereby realizing that the light collecting head collects optical signals for the reagent card detecting window positioned below the light collecting head; and the top of the light collection head is also provided with a shielding cover.

15. The fully automatic fluoroimmunoassay analyzer of claim 14, wherein a reagent card detection tray rotating motor is connected to the reagent card detection tray for driving the reagent card detection tray to rotate; a plurality of reagent screens are uniformly distributed on the reagent card detection plate and used for placing reagent cards.

16. The automatic fluoroimmunoassay analyzer of claim 14, wherein the reagent card disposal paddle unit is disposed outside the reagent card detection tray and at a position corresponding to the lighting head, and comprises a reagent card disposal paddle, a reagent card disposal paddle motor, a reagent card disposal paddle block assembly, a reagent card disposal paddle slide rail, and a reagent card disposal paddle motion assembly, wherein the reagent card disposal paddle is connected to the reagent card disposal paddle block assembly, the reagent card disposal paddle motor is connected to the reagent card disposal paddle block assembly, the reagent card disposal paddle block assembly is sleeved on the reagent card disposal paddle motion assembly and the reagent card disposal paddle slide rail, and the reagent card disposal paddle motor drives the reagent card disposal paddle motion assembly to drive the reagent card disposal paddle block assembly to move, thereby driving the reagent card discarding hand shifting block connected with the reagent card discarding hand shifting block component to reciprocate along the reagent card discarding hand shifting slide rail.

17. The automated fluoroimmunoassay analyzer of claim 15, wherein reagent card clips are disposed on both sides of the reagent card holder for clipping both sides of the reagent card to fix the reagent card in the reagent card detection tray.

18. The fully automatic fluoroimmunoassay analyzer of claim 13, wherein a reagent card scanner is disposed above the reagent card transfer chamber; and a cleaning assembly is arranged above the first carrying shifting hand, and a plurality of cleaning liquid bottles are placed on the cleaning assembly through a cleaning liquid bottle support.

19. The full-automatic fluoroimmunoassay analyzer according to claim 1, wherein the sample feeding and discharging device comprises a sample feeding pushing handle structure, a sample feeding transverse pushing and poking handle structure and a sample discharging pushing handle structure; the sample pushing handle structure is used for pushing a sample tube placed on a sample platform of the sample inlet and outlet platform into a sample along the longitudinal direction; the sample introduction transverse pushing and poking hand structure is used for pushing the sample tube into a sample introduction along the transverse direction; the sample outlet pushing hand structure is used for pushing the detected sample tube out of the sample outlet platform of the sample inlet and outlet platform to carry out sample outlet.

20. The fully automatic fluoroimmunoassay analyzer according to claim 1, further comprising an emergency sample placement site for placing a sample tube and provided with an emergency detection site and an emergency sample tube detection sensor; the emergency sample placing position is located in the horizontal movement direction of the sampling needle.

21. The full-automatic fluoroimmunoassay analyzer of claim 1, wherein the support is further coated with a shell, and the shell is provided with a display screen; the side surface of the shell is also provided with a cleaning liquid bottle inlet, a reagent cassette, a diluting liquid bottle inlet and a waste receiving barrel, the full-automatic fluorescence immunoassay analyzer is also provided with a built-in printer, and a paper outlet of the built-in printer is arranged on the shell; the shell is also provided with an observation window and an item code scanner.

22. A sample detection method is characterized by comprising a normal detection process, wherein the normal detection process comprises the following steps:

(3) opening an emergency sample placing position, inserting an emergency sample tube into an emergency sample detection position, and starting a detection process after detecting that the emergency sample is inserted; the reagent card is pulled out from the reagent card bin and carried to a reagent card detection plate, a sampling needle dilution needle module is started to sample the sample to a reagent card sample adding port, a heating module is started to keep the reagent card in a set temperature for incubation; after the incubation reaction is finished, rotating the reagent card to be detected to the lower part of a lighting head of the optical detection module through the rotation of the reagent card detection disc, and collecting optical signals; or in the process of normal detection process, executing the step (3) in the emergency detection process.