CN113564046B - Auxiliary dismounting mechanism and dismounting system of cell phenotype control device - Google Patents

Auxiliary dismounting mechanism and dismounting system of cell phenotype control device Download PDF

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Publication number
CN113564046B
CN113564046B CN202110829508.2A CN202110829508A CN113564046B CN 113564046 B CN113564046 B CN 113564046B CN 202110829508 A CN202110829508 A CN 202110829508A CN 113564046 B CN113564046 B CN 113564046B
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arm
light source
control box
source control
trigger
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CN113564046A (en
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王雷
郦野
金帆
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Shenzhen Institute of Advanced Technology of CAS
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Shenzhen Institute of Advanced Technology of CAS
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Priority to PCT/CN2021/138002 priority patent/WO2023000590A1/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/12Well or multiwell plates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/46Means for fastening
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/50Means for positioning or orientating the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/52Mobile; Means for transporting the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/06Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48Automatic or computerized control

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Abstract

The invention provides an auxiliary dismounting mechanism and dismounting system of a cell phenotype control device, comprising: the support seat is transversely arranged; the placing table is connected to the supporting seat through a longitudinally telescopic elastic piece and is used for placing the cell phenotype control device; at least one lever assembly pivotably connected to the support base to form a fulcrum, the lever assembly clamping or unclamping the light source control box on the placement table by pivoting about the fulcrum; a trigger configured to drive the lever assembly to pivot toward the chucking light source control box when pressed down by the placement table; and a locking member connected to the support base and configured to releasably lock the trigger member when the lever assembly clamps the light source control box, the lever assembly releasing the light source control box after the locking member releases the trigger member. The invention realizes the automatic disassembly of the cell culture plate of the cell phenotype control device, does not need manual operation of an experimenter, and improves the experimental efficiency.

Description

Auxiliary dismounting mechanism and dismounting system of cell phenotype control device
Technical Field
The invention relates to the technical field of incubators, in particular to an auxiliary dismounting mechanism and dismounting system of a cell phenotype control device.
Background
A cell phenotype control device, such as the cell phenotype control device disclosed in Chinese patent application No. CN202011476070.6, mainly comprises a cell culture plate and a light source control box, wherein the cell culture plate is sleeved outside the top of the light source control box. During actual use, it is often necessary to remove the cell culture plates separately to allow for additional treatment of the cells in the cell culture plates. At present, the disassembly of the cell culture plate is completed by manual operation of an experimenter, so that automatic disassembly is difficult to realize, and the requirement of an automatic biological experiment flow cannot be met.
Disclosure of Invention
The invention aims to provide an auxiliary dismounting mechanism and dismounting system of a cell phenotype control device, so as to solve the problem that a cell culture plate is difficult to automatically dismount.
In order to achieve the above object, the present invention provides an auxiliary dismounting mechanism of a cell phenotype control device, the cell phenotype control device includes a cell culture plate and a light source control box which are clamped, the auxiliary dismounting mechanism defines a transverse direction and a longitudinal direction, and the auxiliary dismounting mechanism includes: the support seat is arranged along the transverse direction; a placement stage connected to the support base through a longitudinally stretchable elastic member for placing the cell phenotype control device; at least one lever assembly pivotably connected to the support base to form a fulcrum, the lever assembly clamping or unclamping a light source control box on the placement table by pivoting about the fulcrum; a trigger configured to drive the lever assembly to pivot in a direction of chucking the light source control box when being pressed down by the placement table; a locking member connected to the support base and configured to releasably lock the trigger member when the lever assembly clamps the light source control box, the lever assembly releasing the light source control box after the locking member releases the trigger member.
The auxiliary dismounting mechanism as described above, wherein the locking member is configured to lock or release the trigger member when pressed by the trigger member, the pressing being from the pressing down of the trigger member by the placement table.
The auxiliary dismounting mechanism is characterized in that the locking piece is a pressing lock catch.
The auxiliary dismounting mechanism comprises a lying arm and a standing arm, wherein one end of the lying arm is a pressed end, the other end of the lying arm is connected with the standing arm, a part between two ends of the lying arm is pivotably connected to the supporting seat to form the supporting point, the triggering piece is longitudinally movably arranged between the pressed end and the placing table, the standing arm is located in the lateral direction of the placing table, and the triggering piece drives the standing arm to pivot towards the direction of clamping the light source control box by pressing down the pressed end.
The auxiliary dismounting mechanism is characterized in that the trigger piece limits the lying arm in a state that the trigger piece is locked by the locking piece, so that the standing arm is kept in a state of clamping the light source control box; after the locking piece releases the triggering piece, the triggering piece releases the limit of the lying arm, and the vertical arm pivots to the supporting seat by means of self gravity so as to automatically loosen the light source control box.
The auxiliary dismounting mechanism is characterized in that the supporting seat is fixedly provided with the guide post for guiding the placing table and the triggering piece to longitudinally move, the placing table and the triggering piece are respectively sleeved outside the guide post in a longitudinally sliding manner, and the placing table, the triggering piece and the lever assembly are sequentially propped against each other from top to bottom in the longitudinal direction.
The auxiliary dismounting mechanism comprises a lantern ring, and an inserting part and a triggering part which are fixed on the outer side of the lantern ring, wherein the lantern ring is longitudinally sleeved outside the guide post in a sliding mode, the number of the inserting part and the number of the locking parts are equal and longitudinally correspond, the inserting parts are respectively used for inserting the locking parts, the number of the triggering part and the number of the lever assemblies are equal and longitudinally correspond, and the triggering parts are respectively used for pressing down the lever assemblies so as to drive the lever assemblies to synchronously pivot.
The auxiliary dismounting mechanism comprises a placing plate for placing the cell phenotype control device and a sleeve fixed at the bottom of the placing plate, wherein the placing plate is connected to the supporting seat through the elastic piece, the sleeve is longitudinally sleeved outside the guide column in a sliding manner and abuts against the trigger piece, and the placing table presses down the trigger piece through the sleeve.
The auxiliary dismounting mechanism is characterized in that the flange is arranged on the outer wall of the light source control box, the lever assembly comprises a clamping block, and the clamping block tightly clamps the light source control box by tightly pressing the flange.
The auxiliary dismounting mechanism comprises two lever assemblies which are arranged opposite to each other, and the trigger piece can drive the two lever assemblies to synchronously pivot towards the direction of clamping the light source control box, so that the two lever assemblies can jointly clamp the light source control box from two opposite sides of the light source control box.
The auxiliary disassembly and assembly mechanism comprises the lever assemblies, wherein each lever assembly further comprises a limiting frame, and the limiting frames of the two lever assemblies limit the position of the cell culture plate in the transverse direction under the condition that the two lever assemblies clamp the light source control box together, so that the cell culture plate and the light source control box are aligned in the longitudinal direction.
The auxiliary dismounting mechanism comprises a lying arm and a standing arm which are connected in a pivotable manner, a tensioning spring is connected between the lying arm and the standing arm, the lying arm is connected to the supporting seat in a pivotable manner so as to enable the trigger piece to be pressed down, the standing arm is used for clamping the light source control box, and in a state that the trigger piece is locked by the locking piece, the placing table is used for enabling the trigger piece to drive the lying arm to pivot in a direction away from the standing arm by pressing down the trigger piece, and meanwhile the trigger piece is enabled to press the locking piece until the trigger piece is released by the locking piece.
The auxiliary dismounting mechanism is characterized in that a limiting piece is clamped between the lying arm and the vertical arm, and the limiting piece limits the minimum included angle between the lying arm and the vertical arm.
The auxiliary dismounting mechanism comprises the limiting frame of one lever assembly, wherein the limiting frame of the other lever assembly comprises a third transverse arm, the third transverse arm is opposite to the first transverse arm and is parallel to the first transverse arm, and the two second transverse arms are connected to the opposite ends of the first transverse arm and are parallel to each other.
The invention also provides a disassembly and assembly system which comprises a mechanical arm and the auxiliary disassembly and assembly mechanism, wherein the mechanical arm is provided with clamping jaws, and the clamping jaws can apply pressure to the placing table and clamp the cell culture plate.
The auxiliary dismounting mechanism and dismounting system of the cell phenotype control device have the characteristics and advantages that:
1. the auxiliary dismounting mechanism adopts the pivotable lever assembly to clamp the light source control box, adopts the trigger piece to trigger the lever assembly to pivot, adopts the locking piece to lock the trigger piece so as to position the lever assembly, is convenient for the mechanical arm to dismount the cell culture plate from the light source control box and mount the cell culture plate on the light source control box, realizes automatic dismounting, does not need manual operation of an experimenter, and improves experimental efficiency;
2. according to the invention, the locking piece is set to be locked and unlocked in a pressing mode, and the locking and unlocking of the trigger piece can be synchronously completed by means of the force application of disassembling and installing the cell culture plate, so that the disassembly and assembly are quicker and more efficient, and the experimental efficiency is further improved;
3. according to the invention, the two lever assemblies are arranged to jointly clamp the light source control box from the two opposite sides of the light source control box, so that the clamping stability is improved;
4. according to the invention, the elastic piece is arranged to support the placing table, so that the placing table can be longitudinally moved, the downward pressure of the mechanical arm on the cell phenotype control device can be transmitted to the trigger piece, in addition, the elastic piece is matched with the clamping block of the lever assembly, so that the flange on the outer wall of the light source control box can be clamped, and the lever assembly can clamp the light source control box.
Drawings
The following drawings are only for purposes of illustration and explanation of the present invention and are not intended to limit the scope of the invention. Wherein:
FIG. 1 is a schematic perspective view of an auxiliary assembly and disassembly mechanism according to an embodiment of the present invention;
FIG. 2 is a schematic illustration of a robotic arm used to place a cell phenotype control device on the auxiliary disassembly and assembly mechanism of FIG. 1;
FIG. 3 is a schematic perspective view of the cell phenotype control apparatus of FIG. 2 placed on an auxiliary disassembly and assembly mechanism;
FIG. 4 is a cross-sectional view of FIG. 3;
FIG. 5 is a schematic view of the auxiliary attachment and detachment mechanism of FIG. 4 with the placement table removed;
fig. 6 and 7 are perspective views of the structure of fig. 5;
FIG. 8 is a cross-sectional view of the lever assembly clamping the light source control pod after applying pressure to the light source control pod with the robotic arm;
FIG. 9 is a cross-sectional view of the lever assembly of FIG. 8 clamping a light source control cartridge;
FIG. 10 is a cross-sectional view of the auxiliary attachment and detachment mechanism of FIG. 9;
FIG. 11 is a schematic perspective view of a lever assembly of the present invention;
FIG. 12 is a schematic perspective view of another lever assembly of the present invention;
FIG. 13 is a schematic perspective view of a lying arm of the present invention;
FIG. 14 is a schematic perspective view of a neutral arm according to the present invention;
FIG. 15 is a schematic perspective view of a spacing member according to the present invention;
FIG. 16 is a schematic perspective view of a trigger member of the present invention;
FIG. 17 is a schematic perspective view of a locking element of the present invention;
FIG. 18 is a schematic perspective view of an elastic member according to the present invention;
fig. 19 and 20 are schematic views showing a perspective structure of a placing table in the present invention;
fig. 21 is a schematic perspective view of a support base according to the present invention.
Reference numerals for main elements:
100. an auxiliary dismounting mechanism;
101. a support base; 102. a placement table; 103. a lever assembly; 104. a trigger; 105. a locking member;
106. an elastic member; 107. a fulcrum; 108. a lying arm; 109. a vertical arm; 110. a compression end; 111. a guide post;
112. a collar; 113. an insertion section; 114. a trigger part; 115. placing a plate; 116. a guide slope;
117. a guide rail; 118. a first chute; 119. a second chute; 120. a cylinder; 121. a spring;
122. a top column; 123. a clamping block; 124. a limiting frame; 125. tensioning the spring; 126. a limiting piece;
127. a chamfering structure; 128. a first cross arm; 129. a second cross arm; 130. a third cross arm;
131. a vertical plate; 132. an avoidance groove; 133. a mounting ring; 134. a mounting base; 135. a mounting plate;
136. a sleeve; 200. a cell culture plate; 300. a light source control box; 301. a flange;
400. a mechanical arm; 401. clamping jaw.
Detailed Description
For a clearer understanding of technical features, objects, and effects of the present invention, a specific embodiment of the present invention will be described with reference to the accompanying drawings. Wherein the adjective or adverb modifiers "upper" and "lower," "top" and "bottom," "inner" and "outer," "transverse" and "longitudinal" are used merely to facilitate relative reference between groups of terms, and do not describe any particular directional limitation on the modified term. In the description of the present invention, unless otherwise indicated, the meaning of "a plurality" is two or more. In the description of the present invention, unless otherwise indicated, the term "coupled" is to be interpreted broadly, and for example, may be fixedly coupled, may be detachably coupled, may be directly coupled, and may be indirectly coupled through an intermediate medium, so that it will be apparent to those skilled in the art that the specific meaning of the term in this patent may be understood in light of the specific circumstances.
Embodiment one
As shown in fig. 1, the present invention provides an auxiliary disassembly and assembly mechanism 100 of a cell phenotype control apparatus, the cell phenotype control apparatus comprises a cell culture plate 200 and a light source control box 300 (shown in fig. 2 and 3) which are clamped, the auxiliary disassembly and assembly mechanism 100 is used for disassembling the cell culture plate 200 from the light source control box 300 or mounting the cell culture plate 200 on the light source control box 300, the auxiliary disassembly and assembly mechanism 100 defines a transverse direction and a longitudinal direction, as shown in fig. 1, 6 and 7, the auxiliary disassembly and assembly mechanism 100 comprises a supporting seat 101, a placing table 102, at least one lever assembly 103, a trigger piece 104 and a locking piece 105, the supporting seat 101 is arranged along the transverse direction, the placing table 102 is used for placing the cell phenotype control apparatus, the placing table 102 is connected to the supporting seat 101 through a longitudinally telescopic elastic piece 106, namely the placing table 102 can longitudinally move, the lever assembly 103 is pivotally connected to the support base 101 to form a pivot 107, the lever assembly 103 clamps (as shown in fig. 9) or releases (as shown in fig. 4) the light source control box 300 on the placement table 102 by pivoting about the pivot 107, the trigger 104 is configured to drive the lever assembly 103 to pivot in a direction to clamp the light source control box 300 when pressed down by the placement table 102, such that the lever assembly 103 clamps the light source control box 300 (as shown in fig. 8 and 9), the locking member 105 is connected to the support base 101 and is configured to releasably lock the trigger 104 when the lever assembly 103 clamps the light source control box 300, such that the lever assembly 103 maintains a state of clamping the light source control box 300, and the trigger 104 contacts a limit of the lever assembly 103 after the locking member 105 releases the trigger 104, and the lever assembly 103 releases the light source control box 300.
The auxiliary dismounting mechanism 100 of the invention is used together with a mechanical arm 400, and the mechanical arm 400 is provided with a clamping jaw 401.
The operation of removing the cell culture plate 200 is as follows:
step S11: as shown in fig. 2, the gripping jaw 401 of the mechanical arm 400 grips the light source control box 300, and places the light source control box 300 on the placing table 102;
step S12: as shown in fig. 8, the clamping jaw 401 of the mechanical arm 400 clamps the light source control box 300, applies a downward longitudinal pressure to the light source control box 300, so that the light source control box 300 drives the placement table 102 to move downwards, the placement table 102 presses down the trigger piece 104, the trigger piece 104 drives the lever assembly 103 to pivot towards the direction of clamping the light source control box 300 until the lever assembly 103 clamps the light source control box 300, and meanwhile, the locking piece 105 locks the trigger piece 104, and at the moment, the locking piece 105 limits the trigger piece 104, so that the lever assembly 103 keeps clamping the light source control box 300, and the light source control box 300 is positioned on the placement table 102;
step S13: the clamping jaw 401 of the mechanical arm 400 clamps the cell culture plate 200, and the cell culture plate 200 is removed from the light source control box 300, so that the disassembly is completed.
The operation of mounting the cell culture plate 200 is as follows:
step S21: the clamping jaw 401 of the mechanical arm 400 clamps the cell culture plate 200, and places the cell culture plate 200 on the light source control box 300;
step S22: the clamping jaw 401 of the mechanical arm 400 clamps the cell culture plate 200, and presses down the cell culture plate 200, so that the cell culture plate 200 and the light source control box 300 are clamped, and the installation is completed.
In one embodiment, as shown in fig. 8, the locking member 105 is configured to lock or release (i.e. unlock) the triggering member 104 when pressed by the triggering member 104, and the pressing of the triggering member 104 against the locking member 105 comes from the pressing down of the triggering member 104 by the placement table 102, so that the locking and unlocking of the triggering member 104 are completed synchronously by means of the force applied by the detachment and installation of the cell culture plate 200, which is ingenious in design and makes the detachment faster.
With the auxiliary dismounting mechanism 100 of the present embodiment, in step S12, the placement table 102 presses down the trigger member 104, the trigger member 104 drives the lever assembly 103 to pivot in the direction of clamping the light source control box 300 while pressing the lock member 105, and the trigger member 104 is locked by the lock member 105 while the lever assembly 103 clamps the light source control box 300; in step S22, the clamping jaw 401 of the mechanical arm 400 presses down the cell culture plate 200, the placement table 102 presses down the trigger 104, the trigger 104 presses the locking member 105, and the trigger 104 is released by the locking member 105 while the cell culture plate 200 and the light source control box 300 are clamped, so that the lever assembly 103 releases the light source control box 300, and the assembled cell phenotype control device is separated from the auxiliary dismounting mechanism 100 (see fig. 4).
In an embodiment, as shown in fig. 17, the locking member 105 is a push button, and the structure thereof is the prior art, so that the description is omitted.
In an embodiment, as shown in fig. 1, 11 and 12, the lever assembly 103 includes a horizontal arm 108 and a vertical arm 109, one end of the horizontal arm 108 is a pressed end 110, the other end of the horizontal arm 108 is connected to the vertical arm 109, that is, the lever assembly 103 has a generally L-shaped structure, a portion between two ends of the horizontal arm 108 is pivotally connected to the supporting seat 101 to form a fulcrum 107, the trigger 104 is longitudinally movably disposed between the pressed end 110 and the placement table 102, the vertical arm 109 is located at a lateral side of the placement table 102, and the trigger 104 drives the vertical arm 109 to pivot toward a direction of clamping the light source control box 300 by pressing the pressed end 110 so as to implement clamping of the light source control box 300.
Specifically, in a state in which the trigger 104 is locked by the lock 105, the trigger 104 positions the horizontal arm 108 so that the vertical arm 109 is held in a state of clamping the light source control box 300, and at this time, the horizontal arm 108 is supported by the fulcrum 107 and the trigger 104 together, and the horizontal arm 108 is in a substantially horizontal state (as shown in fig. 9 and 10); after the trigger 104 is released from the locking member 105, the trigger 104 releases the limit of the lying arm 108, and the standing arm 109 pivots onto the supporting seat 101 by its own weight and automatically releases the light source control box 300, at this time, the lying arm 108 is supported by the supporting point 107 and the supporting seat 101 together, and the lying arm 108 is in an inclined state (as shown in fig. 6 and 7).
In an embodiment, as shown in fig. 6, 7 and 21, a guide post 111 for guiding the placement table 102 and the trigger piece 104 to move longitudinally is fixed on the supporting seat 101, the placement table 102 and the trigger piece 104 are respectively sleeved outside the guide post 111 in a longitudinally sliding manner, and the placement table 102, the trigger piece 104 and the lever assembly 103 are propped against each other sequentially from top to bottom in the longitudinal direction.
Further, as shown in fig. 6, 7 and 16, the trigger 104 includes a collar 112, and an insertion portion 113 and a trigger portion 114 fixed on the outer side of the collar 112, where the collar 112 is longitudinally slidably sleeved outside the guide post 111, the number of the insertion portions 113 and the locking members 105 are equal and longitudinally corresponding, each insertion portion 113 is respectively used for being inserted into each locking member 105, the number of the trigger portions 114 and the lever assemblies 103 are equal and longitudinally corresponding, and each trigger portion 114 is respectively used for pressing down each lever assembly 103 so as to drive each lever assembly 103 to pivot synchronously (as shown in fig. 4 and 5).
Further, as shown in fig. 4, 10, 19 and 20, the placement stage 102 includes a placement plate 115 for placing the cell phenotype control device and a sleeve 136 fixed to the bottom of the placement plate 115, the placement plate 115 is connected to the support base 101 through an elastic member 106, the sleeve 136 is longitudinally slidably sleeved outside the guide post 111 and abuts against the trigger 104, and the placement stage 102 presses down the trigger 104 through the sleeve 136.
Further, as shown in fig. 1 and 19, guide inclined surfaces 116 are provided around the top surface of the placement plate 115 to guide the light source control box 300 to the middle region of the placement plate 115 when the robot arm 400 places the light source control box 300 on the placement plate 115, preventing the position of the light source control box 300 from being shifted.
Further, as shown in fig. 16, 20 and 21, a plurality of guide rails 117 are disposed on the outer wall of the guide post 111, the plurality of guide rails 117 are arranged at intervals along the circumferential direction of the guide post 111, each guide rail 117 extends longitudinally, a plurality of first sliding grooves 118 respectively matched with the plurality of guide rails 117 in a sliding manner are disposed on the side wall of the collar 112 of the trigger piece 104, a plurality of second sliding grooves 119 respectively matched with the plurality of guide rails 117 in a sliding manner are disposed on the side wall of the sleeve 136 of the placement table 102, and the extending directions of the first sliding grooves 118 and the second sliding grooves 119 are all longitudinal, so that the guide post 111 can guide the trigger piece 104 and the placement table 102 longitudinally.
In an embodiment, as shown in fig. 4 and 18, the elastic member 106 includes a cylinder 120 fixed on the supporting base 101, a spring 121 disposed in the cylinder 120, and a jack 122 extending into the cylinder 120, one end of the jack 122 abuts against the spring 121, and the other end of the jack 122 contacts the placing plate 115 of the placing table 102 to form an elastic support for the placing table 102, so that the placing table 102 can move longitudinally.
In an embodiment, as shown in fig. 3, 4 and 9, a flange 301 is provided on an outer wall of the light source control box 300, and the lever assembly 103 includes a clamping block 123, where the clamping block 123 tightly clamps the light source control box 300 through the pressing flange 301.
Specifically, in step S12, the position where the clamping jaw 401 of the mechanical arm 400 clamps the light source control box 300 is the position where the bottom of the clamping jaw 401 abuts against the top of the flange 301, so that the clamping jaw 401 of the mechanical arm 400 applies downward pressure to the light source control box 300 by pressing down the flange 301, the light source control box 300 drives the placement table 102 to move downward, when the trigger piece 104 is locked by the locking piece 105, the clamping block 123 just pivots above the flange 301 along with the standing arm 109, then the clamping jaw 401 releases the light source control box 300, and the placement table 102 moves upward under the restoring force of the elastic piece 106 until the flange 301 contacts the clamping block 123 and is pressed by the clamping block 123 (as shown in fig. 9).
Therefore, in the present embodiment, the clamping of the light source control box 300 by the lever assembly 103 is achieved by the clamping block 123 of the lever assembly 103 pressing the light source control box 300 from above the flange 301, and the elastic member 106 pressing the light source control box 300 from the bottom of the placing table 102.
In an embodiment, as shown in fig. 9, the auxiliary dismounting mechanism 100 includes two lever assemblies 103 disposed opposite to each other, and the trigger member 104 can drive the two lever assemblies 103 to pivot synchronously toward the direction of clamping the light source control box 300, so that the two lever assemblies 103 jointly clamp the light source control box 300 from opposite sides of the light source control box 300, thereby improving the clamping stability.
In the example of fig. 6 and 7, the two locking members 105 are arranged at 90 ° intervals around a longitudinal center line where the trigger member 104 is disposed, the trigger member 104 includes two trigger portions 114 and two insertion portions 113 arranged at 90 ° intervals around the longitudinal center line, the two trigger portions 114 and the two insertion portions 113 respectively correspond to the pressed ends 110 of the two lever members 103 in the longitudinal direction, and the two insertion portions 113 respectively correspond to the two locking members 105 in the longitudinal direction, so that the trigger member 104 inserts the two locking members 105 while driving the two lever members 103 to pivot synchronously in the direction of gripping the light source control box 300, and the two locking members 105 lock the trigger member 104 while the two lever members 103 grip the light source control box 300, thereby synchronously spacing the two lever members 103.
In one embodiment, as shown in fig. 6 and 7, each lever assembly 103 further includes a limiting frame 124, and in a state that the two lever assemblies 103 jointly clamp the light source control box 300, the limiting frames 124 of the two lever assemblies 103 jointly limit the position of the cell culture plate 200 in the lateral direction, so that the cell culture plate 200 and the light source control box 300 are aligned in the longitudinal direction.
In the past when installing cell culture plate 200, the rib of cell culture plate 200 below is difficult to accurately insert in the draw-in groove of light source control box 300 top, leads to the unable inseparable laminating of up end of the interior spotlight board of cell culture plate 200 of light source control box 300 after installing, leads to the light source between the micropore to interfere with each other, and this embodiment is through setting up spacing 124, under the state of lever assembly 103 chucking light source control box 300, adopt clamping jaw 401 of arm 400 to place cell culture plate 200 between two spacing 124, two spacing 124 play the effect of rectifying to cell culture plate 200's position, make cell culture plate 200 can closely laminate with the spotlight board of below, avoid the problem emergence of light source interference between the micropore.
When the cell culture plate 200 is aligned with the light source control box 300, the side surfaces of the cell culture plate 200 are attached to the inner side surfaces of the two limiting frames 124, in this case, if the clamping jaw 401 of the mechanical arm 400 presses down the cell culture plate 200, the placing table 102 will be driven to move downward, when the placing table 102 contacts the trigger piece 104, if the placing table is pressed down continuously, the pressed end 110 of the lever assembly 103 will have a tendency to rotate downward, but because the limiting frames 124 of the lever assembly 103 are attached to the cell culture plate 200, the limiting frames 124 and the vertical arms 109 will be blocked by the cell culture plate 200 and cannot rotate along with the pressed end 110.
To avoid the stop 124 blocking downward rotation of the pressed end 110, in one embodiment, as shown in fig. 11 and 12, the inventors design the upright arm 109 and the horizontal arm 108 of the lever assembly 103 to be pivotally connected to allow relative rotation therebetween, and a tension spring 125 is connected between the horizontal arm 108 and the upright arm 109.
When the cell culture plate 200 is installed, the vertical arm 109 is in a state of clamping the light source control box 300, the trigger piece 104 is in a state of being locked by the locking piece 105 (as shown in fig. 9), after the cell culture plate 200 is placed between the two limiting frames 124 by the clamping jaw 401 of the mechanical arm 400, the cell culture plate 200 is pressed down by the clamping jaw 401 of the mechanical arm 400, the cell culture plate 200 is clamped with the light source control box 300, the placing table 102 is driven to move downwards, the placing table 102 presses the trigger piece 104, the trigger piece 104 drives the lying arm 108 to pivot in a direction away from the vertical arm 109, the included angle between the lying arm 108 and the vertical arm 109 is increased, the tensioning spring 125 is stretched, the trigger piece 104 presses the locking piece 105, the trigger piece 104 is released after the locking piece 105 is pressed, the limiting of the lying arm 108 is released by the trigger piece 104, the vertical arm 109 is pivoted onto the supporting seat 101 by means of self gravity, and therefore the clamping of the light source control box 300 is released by the clamping jaw 401 of the mechanical arm 400 (see fig. 4), and the cell phenotype device is taken away from the placing table 102.
Further, as shown in fig. 11, 12 and 15, a stopper 126 is interposed between the lying arm 108 and the standing arm 109, and the stopper 126 defines a minimum angle between the lying arm 108 and the standing arm 109, for example, the minimum angle is 90 °. Specifically, for example, the limiting member 126 is fixed to the end of the lying arm 108 near the standing arm 109, and for example, the limiting member 126 is a limiting plate.
Further, as shown in fig. 11 and 12, each of the stoppers 124 is provided with a chamfer structure 127 for guiding the cell culture plate 200, and when the holding jaw 401 of the mechanical arm 400 places the cell culture plate 200 between the two stoppers 124, the chamfer structure 127 on the stopper 124 guides the cell culture plate 200 to move to a position between the two stoppers 124.
Further, as shown in fig. 1, the limiting frame 124 of one lever assembly 103 includes a first cross arm 128 and two second cross arms 129 (as shown in fig. 12) connected at opposite ends of the first cross arm 128 and parallel to each other, and the limiting frame 124 of the other lever assembly 103 includes a third cross arm 130 (as shown in fig. 11), the third cross arm 130 and the first cross arm 128 are opposite to each other and parallel to each other, the first cross arm 128 and the third cross arm 130 are used for limiting the position of the cell culture plate 200 in the left-right direction, and the two second cross arms 129 are used for limiting the position of the cell culture plate 200 in the front-rear direction. The other lever assembly 103 is not provided with a cross arm for limiting the position of the cell culture plate 200 in the front-rear direction, so as to prevent interference to the clamping jaw 401 of the mechanical arm 400, and facilitate the clamping jaw 401 to clamp the cell culture plate 200 from two ends of the third cross arm 130.
Further, as shown in fig. 13 and 14, the horizontal arm 108 and the vertical arm 109 are each channel steel, so that the limiting frame 124, the clamping block 123, the tension spring 125 and the limiting plate are connected with two side walls of the channel steel through pins.
In an embodiment, as shown in fig. 21, the supporting seat 101 is a square plate, four sides of the square plate are respectively connected with a vertical plate 131 to form a box-shaped structure with an open top, and in order to avoid interference between the vertical plate 131 and the clamping jaw 401 of the mechanical arm 400, the vertical plate 131 is configured to have a structure avoiding the groove 132, so as to provide a movement and operation space for the clamping jaw 401 of the mechanical arm 400.
In one embodiment, as shown in fig. 21, four mounting rings 133, two mounting seats 134 and two sets of mounting plates 135 are provided on the supporting base 101, the four mounting rings 133 are used for fixing the four elastic members 106, the mounting seats 134 are used for fixing the locking members 105, and each set of mounting plates 135 is two for connecting the lying arm 108 through the pivot shaft.
Second embodiment
As shown in fig. 2 and 8, the present invention further provides a disassembling and assembling system, which includes a mechanical arm 400 and the auxiliary disassembling and assembling mechanism 100 according to the first embodiment, the mechanical arm 400 has a clamping jaw 401, and the clamping jaw 401 can apply pressure to the placement table 102 and can clamp the cell culture plate 200.
When the cell culture plate 200 is detached, the holding jaw 401 holds the light source control box 300 and applies pressure to the placement stage 102 by applying pressure to the light source control box 300, and when the cell culture plate 200 is mounted, the holding jaw 401 holds the cell culture plate 200 and applies pressure to the placement stage 102 by applying pressure to the cell culture plate 200.
The structure, the working principle and the beneficial effects of the auxiliary dismounting mechanism 100 in this embodiment are the same as those of the first embodiment, and are not described here again.
The foregoing is illustrative of the present invention and is not to be construed as limiting the scope of the invention. Any equivalent changes and modifications can be made by those skilled in the art without departing from the spirit and principles of this invention, and are intended to be within the scope of this invention. It should be noted that, the components of the present invention are not limited to the above-mentioned overall application, and each technical feature described in the specification of the present invention may be selected to be used singly or in combination according to actual needs, so that other combinations and specific applications related to the present invention are naturally covered by the present invention.

Claims (13)

1. An auxiliary disassembly and assembly mechanism for a cell phenotype control device, the cell phenotype control device comprising a cell culture plate and a light source control box which are clamped, the auxiliary disassembly and assembly mechanism defining a transverse direction and a longitudinal direction, the auxiliary disassembly and assembly mechanism comprising:
the support seat is arranged along the transverse direction;
a placement stage connected to the support base through a longitudinally stretchable elastic member for placing the cell phenotype control device;
two lever assemblies disposed opposite to each other and pivotably connected to the support base to form a fulcrum, the lever assemblies clamping or unclamping the light source control box on the placement table by pivoting about the fulcrum;
a trigger configured to drive the two lever assemblies to pivot synchronously in a direction of clamping the light source control box when being pressed down by the placement table, so that the two lever assemblies jointly clamp the light source control box from opposite sides of the light source control box;
a locking member connected to the support base and configured to releasably lock the trigger member when the lever assembly clamps the light source control box, the lever assembly releasing the light source control box after the locking member releases the trigger member;
the lever assembly comprises a lying arm and a standing arm, one end of the lying arm is a pressed end, the other end of the lying arm is connected with the standing arm, a part between two ends of the lying arm is pivotally connected to the supporting seat to form a supporting point, the triggering piece is longitudinally movably arranged between the pressed end and the placing table, the standing arm is located in the lateral direction of the placing table, and the triggering piece drives the standing arm to pivot towards the direction of clamping the light source control box by pressing down the pressed end.
2. The auxiliary attachment and detachment mechanism of claim 1, wherein the locking member is configured to lock or release the trigger member upon being pressed by the trigger member, the pressing being from a depression of the trigger member by the placement table.
3. The auxiliary dismounting mechanism of claim 2, wherein the locking member is a push lock.
4. The auxiliary dismounting mechanism as claimed in claim 1, wherein said trigger member limits said lying arm in a state where said trigger member is locked by said locking member, so that said standing arm is kept in a state of gripping said light source control box; after the locking piece releases the triggering piece, the triggering piece releases the limit of the lying arm, and the vertical arm pivots to the supporting seat by means of self gravity so as to automatically loosen the light source control box.
5. The auxiliary dismounting mechanism as claimed in claim 1, wherein a guide post for guiding the placement table and the trigger piece to move longitudinally is fixed on the support base, the placement table and the trigger piece are respectively sleeved outside the guide post in a longitudinally sliding manner, and the placement table, the trigger piece and the lever assembly are propped against each other sequentially from top to bottom in the longitudinal direction.
6. The auxiliary dismounting mechanism as set forth in claim 5, wherein the trigger member comprises a collar, and an insertion portion and a trigger portion fixed to an outer side of the collar, the collar is slidably sleeved outside the guide post in a longitudinal direction, the number of the insertion portion and the number of the locking members are equal and longitudinally correspond, the insertion portion is respectively used for inserting the locking members, the number of the trigger portion and the number of the lever assemblies are equal and longitudinally correspond, and the trigger portion is respectively used for pressing down the lever assemblies to drive the lever assemblies to pivot synchronously.
7. The auxiliary disassembling and assembling mechanism according to claim 5, wherein the placing table comprises a placing plate for placing the cell phenotype control device and a sleeve fixed at the bottom of the placing plate, the placing plate is connected to the supporting seat through the elastic piece, the sleeve is longitudinally slidably sleeved outside the guide post and abuts against the triggering piece, and the placing table presses down the triggering piece through the sleeve.
8. The auxiliary dismounting mechanism as claimed in claim 1, wherein a flange is provided on an outer wall of the light source control box, and the lever assembly includes a fixture block, and the fixture block is used for clamping the light source control box by pressing the flange.
9. The auxiliary disassembling and assembling mechanism according to claim 1, wherein each of the lever assemblies further comprises a limiting frame, and in a state that the two lever assemblies clamp the light source control box together, the limiting frames of the two lever assemblies limit the position of the cell culture plate in the transverse direction together so that the cell culture plate and the light source control box are aligned in the longitudinal direction.
10. The auxiliary dismounting mechanism as claimed in claim 9, wherein the lever assembly includes a lying arm and a standing arm pivotally connected, a tension spring is connected between the lying arm and the standing arm, the lying arm is pivotally connected to the support base for pressing down the trigger member, the standing arm is used for clamping the light source control box, and in a state where the standing arm clamps the light source control box and the locking member locks the trigger member, the placement table makes the trigger member drive the lying arm to pivot in a direction away from the standing arm by pressing down the trigger member, and simultaneously makes the trigger member press the locking member until the locking member releases the trigger member.
11. The auxiliary disassembly and assembly mechanism of claim 10, wherein a stop is interposed between the lying arm and the standing arm, the stop defining a minimum included angle between the lying arm and the standing arm.
12. The auxiliary disassembling and assembling mechanism according to claim 9, wherein the limit frame of one lever assembly comprises a first cross arm and two second cross arms which are connected to opposite ends of the first cross arm and are parallel to each other, and the limit frame of the other lever assembly comprises a third cross arm which is opposite to the first cross arm and is parallel to each other.
13. A disassembly and assembly system comprising a robotic arm having a jaw capable of applying pressure to the placement table and capable of gripping the cell culture plate and an auxiliary disassembly and assembly mechanism according to any one of claims 1 to 12.
CN202110829508.2A 2021-07-22 2021-07-22 Auxiliary dismounting mechanism and dismounting system of cell phenotype control device Active CN113564046B (en)

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113564046B (en) * 2021-07-22 2023-08-22 深圳先进技术研究院 Auxiliary dismounting mechanism and dismounting system of cell phenotype control device
LU503075B1 (en) * 2022-11-17 2024-05-17 Univ Du Luxembourg Microphysiological system

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110366591A (en) * 2016-12-01 2019-10-22 伯克利之光生命科技公司 Wellhole plate incubator
CN210314274U (en) * 2019-07-03 2020-04-14 深圳普瑞材料技术有限公司 Cytotoxicity irradiator
CN112457984A (en) * 2020-12-15 2021-03-09 深圳先进技术研究院 Cell phenotype control device
WO2021138963A1 (en) * 2020-01-12 2021-07-15 南京岚煜生物科技有限公司 Fully automatic chemiluminescent immune analyzer

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3924595B2 (en) * 2001-12-07 2007-06-06 株式会社アステム Multiwell plate transfer device
US7670555B2 (en) * 2006-09-08 2010-03-02 Rex A. Hoover Parallel gripper for handling multiwell plate
US8221697B2 (en) * 2007-01-12 2012-07-17 Nichols Michael J Apparatus for lidding or delidding microplate
US8691149B2 (en) * 2007-11-06 2014-04-08 Abbott Laboratories System for automatically loading immunoassay analyzer
DE102011109332A1 (en) * 2011-08-03 2013-02-07 Eppendorf Ag Laboratory apparatus and method for treating laboratory samples
CN104403945B (en) * 2014-12-09 2017-10-17 常州英德生物科技有限公司 The structure of cell culture board clamp
WO2016208526A1 (en) * 2015-06-22 2016-12-29 東洋製罐グループホールディングス株式会社 Cell culture method, jig for cell culture, and cell culture device
CN206858549U (en) * 2017-06-23 2018-01-09 重庆三峡医药高等专科学校 A kind of Tissue Culture Plate clamping device
WO2019186406A1 (en) * 2018-03-27 2019-10-03 Idea Machine Development Design & Production Ltd. Gripping and mobilizing system
CN113278526B (en) * 2021-05-25 2024-01-26 安徽医学高等专科学校 Multifunctional cell incubator for embryo assembling experiment
CN113564046B (en) * 2021-07-22 2023-08-22 深圳先进技术研究院 Auxiliary dismounting mechanism and dismounting system of cell phenotype control device

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110366591A (en) * 2016-12-01 2019-10-22 伯克利之光生命科技公司 Wellhole plate incubator
CN210314274U (en) * 2019-07-03 2020-04-14 深圳普瑞材料技术有限公司 Cytotoxicity irradiator
WO2021138963A1 (en) * 2020-01-12 2021-07-15 南京岚煜生物科技有限公司 Fully automatic chemiluminescent immune analyzer
CN112457984A (en) * 2020-12-15 2021-03-09 深圳先进技术研究院 Cell phenotype control device

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