CN113466476B - Sample pretreatment method of clinical automatic analyzer - Google Patents
Sample pretreatment method of clinical automatic analyzer Download PDFInfo
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- CN113466476B CN113466476B CN202110775565.7A CN202110775565A CN113466476B CN 113466476 B CN113466476 B CN 113466476B CN 202110775565 A CN202110775565 A CN 202110775565A CN 113466476 B CN113466476 B CN 113466476B
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- 238000002203 pretreatment Methods 0.000 title claims abstract description 15
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 171
- 239000011324 bead Substances 0.000 claims abstract description 70
- 230000007246 mechanism Effects 0.000 claims abstract description 40
- 238000011534 incubation Methods 0.000 claims abstract description 34
- 238000006243 chemical reaction Methods 0.000 claims abstract description 26
- 238000001514 detection method Methods 0.000 claims abstract description 26
- 239000003480 eluent Substances 0.000 claims abstract description 11
- 238000005406 washing Methods 0.000 claims abstract description 8
- 238000004140 cleaning Methods 0.000 claims description 30
- 238000011049 filling Methods 0.000 claims description 30
- 238000012546 transfer Methods 0.000 claims description 29
- 230000010355 oscillation Effects 0.000 claims description 28
- 238000000926 separation method Methods 0.000 claims description 26
- 238000004321 preservation Methods 0.000 claims description 22
- 239000007788 liquid Substances 0.000 claims description 17
- 239000006148 magnetic separator Substances 0.000 claims description 17
- 238000002156 mixing Methods 0.000 claims description 16
- 239000006228 supernatant Substances 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 10
- 238000011084 recovery Methods 0.000 claims description 10
- 238000001294 liquid chromatography-tandem mass spectrometry Methods 0.000 claims description 9
- 238000003805 vibration mixing Methods 0.000 claims description 6
- 238000002347 injection Methods 0.000 claims description 5
- 239000007924 injection Substances 0.000 claims description 5
- 238000007781 pre-processing Methods 0.000 claims description 5
- 238000012742 biochemical analysis Methods 0.000 claims description 3
- 238000003018 immunoassay Methods 0.000 claims description 3
- 238000001179 sorption measurement Methods 0.000 claims description 3
- 239000011521 glass Substances 0.000 claims description 2
- 238000004064 recycling Methods 0.000 claims description 2
- 238000007792 addition Methods 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 4
- 230000004308 accommodation Effects 0.000 description 2
- 238000012864 cross contamination Methods 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000000622 liquid--liquid extraction Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000002414 normal-phase solid-phase extraction Methods 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 239000012716 precipitator Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000006920 protein precipitation Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00722—Communications; Identification
- G01N35/00732—Identification of carriers, materials or components in automatic analysers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/0092—Scheduling
- G01N35/0095—Scheduling introducing urgent samples with priority, e.g. Short Turn Around Time Samples [STATS]
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/025—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/067—Preparation by reaction, e.g. derivatising the sample
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
- G01N2035/00356—Holding samples at elevated temperature (incubation)
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
- G01N35/00722—Communications; Identification
- G01N35/00732—Identification of carriers, materials or components in automatic analysers
- G01N2035/00742—Type of codes
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Abstract
The invention discloses a sample pretreatment method of a clinical automatic analyzer, which comprises the following steps of: the first reaction component consists of a first accommodating disc, a first incubation belt, a first oscillator and a first magnetic attraction separator, the second reaction component consists of a second accommodating disc, a second incubation belt, a second oscillator, a second magnetic attraction separator and a magnetic washing separator, and a reagent storage mechanism, a plurality of manipulators and a pipetting needle, wherein n common reagents can be added to (1), n common reagents can be added to (2) containing precipitants and magnetic bead reagents, two groups of reagents can be added to (3) containing the samples of precipitants and the magnetic bead reagents, the first group of reagents is m common reagents, the samples containing the precipitants, the second group of reagents is n common reagents, the samples of the magnetic bead reagents and the eluents are subjected to pretreatment, and the reagent storage mechanism can ensure a high-flux automatic mode and improve the target detection efficiency.
Description
Technical Field
The invention relates to the technical field of mass spectrum detection equipment, in particular to a sample pretreatment method of a clinical automatic analyzer.
Background
The liquid chromatography tandem mass spectrometry (LC-MS/MS) can add additional analysis capability, and can accurately identify and quantify micro-compounds in complex sample matrixes such as cell and tissue lysate, blood, plasma, urine, oral liquid and the like, so that the liquid chromatography tandem mass spectrometry (LC-MS/MS) has more and more application in clinical application.
However, existing LC-MS/MS perform sample pretreatment mainly by manual operation of a professional laboratory staff. The requirements on the professional skills of operators are high, time and labor are consumed, and risks of poor consistency, cross contamination, biological safety and the like exist. Secondly, different targets are often matched with different reagents and pretreatment modes, including a protein precipitation method, a liquid-liquid extraction method, a solid-phase extraction method and the like. Different processing methods require different functional modules and different workflows, and it is difficult to achieve high throughput automated pretreatment of samples. Particularly for pre-processing where there is a different item interleave, it is more difficult to achieve high throughput automation. The above factors have prevented LC-MS/MS to some extent from developing and applying clinically.
Disclosure of Invention
In order to solve the problems, the invention provides a safe, efficient and widely-used sample pretreatment method of a clinical automatic analyzer, which concretely adopts the following technical scheme:
the invention relates to a sample pretreatment method of a clinical automatic analyzer, which comprises a sample conveying unit, a pretreatment unit and a detection unit which are sequentially connected, wherein the pretreatment unit comprises the following components:
a first reaction assembly, comprising:
the first accommodating disc is an annular disc rotating around the center, and a plurality of sample cup placing positions are arranged on the first accommodating disc;
a first incubation zone disposed below the first receiving tray for maintaining a sample located on the first receiving tray in a constant temperature state;
the first oscillator is arranged below the first accommodating disc or outside the first accommodating disc and is used for uniformly mixing the sample and the reagent; and
the first magnetic separator is arranged below the first accommodating disc and is used for adsorbing magnetic beads in the reagent to separate the magnetic beads from the sample and the reagent;
the second reaction subassembly sets up in first reaction subassembly one side, the second reaction subassembly includes:
the second accommodating disc is an annular disc rotating around the center, and a plurality of sample cup placing positions are arranged on the second accommodating disc;
a second incubation zone disposed below the second holding tray for maintaining the samples on the second holding tray in a constant temperature state;
the second oscillator is arranged below the second accommodating disc or outside the second accommodating disc and is used for uniformly mixing the sample and the reagent;
the second magnetic separator is arranged below the second accommodating disc and is used for adsorbing magnetic beads in the reagent to separate the magnetic beads from the sample and the reagent; and
the magnetic washing separator is arranged at one side of the second accommodating disc and comprises an adsorption mechanism for adsorbing magnetic beads in the reagent, a liquid injection mechanism for washing the magnetic beads and a liquid discharge mechanism;
the reagent storage mechanism comprises a rotating disc, wherein a plurality of groups of reagent placement positions are arranged on the rotating disc, and each group of reagent placement positions comprises a plurality of containing holes which are sequentially arranged along the radius of the rotating disc;
the first manipulator is arranged on one side of the first accommodating disc and is used for feeding empty sample cups into a cup placing position of the first accommodating disc and transferring the sample cups between the cup placing position and the first oscillator;
the second manipulator is arranged between the first accommodating disc and the second accommodating disc and is used for transferring the sample cup from the transfer position of the first accommodating disc to the transfer receiving position of the second accommodating disc;
the first pipetting needle is arranged between the first accommodating disc and the sample conveying unit, and the overlapping part of the first pipetting needle and the first accommodating disc forms a sample adding position;
the second pipetting needle is arranged between the rotating disc and the first accommodating disc, and the overlapping part of the second pipetting needle and the first accommodating disc forms a reagent adding position;
the third pipetting needle is arranged between the rotating disc and the second accommodating disc, and the overlapping part of the third pipetting needle and the second accommodating disc forms another reagent adding position;
the fourth pipetting needle is arranged between the first accommodating disc, the second accommodating disc and the detection unit, the overlapping part of the fourth pipetting needle and the first accommodating disc forms a separation position, the overlapping part of the fourth pipetting needle and the second accommodating disc forms a sample outlet position, and the fourth pipetting needle is used for transferring samples from the first accommodating disc or the second accommodating disc to the detection unit;
the fifth pipetting needle is arranged on one side of the first accommodating disc and overlapped with the first accommodating disc in the cup placing position;
the first magnetic separator is arranged corresponding to the separation position, and the second magnetic separator is arranged corresponding to the sample outlet position;
for the case of adding n (n is more than or equal to 1) common reagents and containing precipitants and magnetic bead reagents into a sample, the sample pretreatment method comprises the following steps:
s1: taking an empty sample cup by a first manipulator and placing the empty sample cup at a cup placing position of a first accommodating disc;
s2: rotating the first accommodating disc to enable the sample cup to be rotated from a cup placing position to a sample adding position, and adding one sample on the sample conveying belt into the sample cup through the first pipetting needle;
s3: rotating the first accommodating disc to enable the sample cup to be transferred from the sample adding position to the reagent adding position, and filling the first common reagent on the rotating disc into the sample cup through the second pipetting needle;
s4: rotating the first accommodating disc to enable the sample cup to be transferred from the reagent adding position to the cup placing position, and transferring the sample cup into the first oscillator through the first manipulator to perform full oscillation;
s5: the sample cup is moved back to the cup position by a first manipulator, and heat preservation cultivation is carried out by a first incubation belt;
s6: sequentially carrying out S3-S5, repeating n times to finish filling, shaking and mixing and heat preservation cultivation of other common reagents and reagents containing precipitants and magnetic beads;
s7: rotating the first accommodating disc to enable the sample cup to be rotated from a cup placing position to a separating position, separating the sample from the magnetic beads under the action of the first magnetic attraction separator, and transferring supernatant to the detection unit through the fourth pipetting needle to finish sample pretreatment;
for the case of adding n (n is more than or equal to 1) common reagents, magnetic bead reagents and eluents into a sample, the sample pretreatment method comprises the following steps:
s1: taking an empty sample cup by a first manipulator and placing the empty sample cup at a cup placing position of a first accommodating disc;
s2: rotating the first accommodating disc to enable the sample cup to be rotated from a cup placing position to a sample adding position, and adding one sample on the sample conveying belt into the sample cup through the first pipetting needle;
s3: rotating the first accommodating disc to enable the sample cup to be transferred from the sample adding position to the reagent adding position, and filling the first common reagent on the rotating disc into the sample cup through the second pipetting needle;
s4: rotating the first accommodating disc to enable the sample cup to be transferred from the reagent adding position to the cup placing position, and transferring the sample cup into the first oscillator through the first manipulator to perform full oscillation;
s5: the sample cup is moved back to the cup position by a first manipulator, and heat preservation cultivation is carried out by a first incubation belt;
s6: sequentially carrying out S3-S5, repeating n times, and completing filling, vibration mixing and heat preservation cultivation of other common reagents and magnetic bead reagents;
s7: rotating the first accommodating disc to enable the sample cup to be transferred from the cup placing position to the transferring position, and transferring the sample cup to the transferring receiving position of the second accommodating disc through the second manipulator;
s8: rotating the second accommodating disc to enable the sample cup to enter the cleaning separation position from the transfer receiving position, and adsorbing magnetic beads mixed into the sample through the magnetic cleaning separator, and cleaning at the same time;
s9: rotating the second accommodating disc to enable the sample cup to be transferred from the cleaning separation position to the reagent adding position, and adding the eluent on the rotating disc into the sample cup through the third pipetting needle;
s10: rotating the second accommodating disc to enable the sample cup to be rotated from the reagent adding position to the oscillation position for full oscillation;
s11: rotating the second accommodating disc to enable the sample cup to rotate out of the oscillation position, and carrying out heat preservation cultivation through the second incubation belt;
s12: rotating the second containing disc to enable the sample cup to rotate to a sample outlet position, layering the sample and magnetic beads mixed in the sample under the action of the second magnetic separator, and transferring supernatant to the detection unit through the fourth pipetting needle to finish sample pretreatment;
aiming at the condition that two groups of reagents are added into a sample, wherein the first group of reagents are m (m is more than or equal to 1) common reagents and contain precipitants, and the second group of reagents are n (n is more than or equal to 1) common reagents, magnetic bead reagents and eluents, the sample pretreatment method comprises the following steps:
s1: taking an empty sample cup X by a first manipulator and placing the empty sample cup X in a cup placing position of a first accommodating disc;
s2: rotating the first accommodating disc to enable the sample cup X to be transferred from the cup placing position to the sample adding position, and adding one sample on the sample conveying belt into the sample cup X through the first pipetting needle;
s3: rotating the first accommodating disc to enable the sample cup X to be transferred from the sample adding position to the reagent adding position, and filling a first common reagent of a first group of reagents on the rotating disc into the sample cup X through the second pipetting needle;
s4: rotating the first accommodating disc to enable the sample cup X to be transferred from the reagent adding position to the cup placing position, and transferring the sample cup X into the first oscillator through the first manipulator to perform full oscillation;
s5: moving the sample cup X back to the cup placing position by a first manipulator, and carrying out heat preservation cultivation by a first incubation belt;
s6: sequentially performing S3-S5, repeating m times to finish the filling, shaking and mixing and heat preservation cultivation of the rest common reagents of the first group and the reagents containing the precipitant and the magnetic beads;
s7: rotating the first accommodating disc to enable the sample cup X to be rotated from a cup placing position to a separating position, separating the sample from the magnetic beads under the action of the first magnetic attraction separator, and then sucking all supernatant through the fifth pipetting needle;
s8: rotating the first accommodating disc, transferring the sample cup X with the magnetic beads from the separation position to the rotation position, and moving the sample cup X and the magnetic beads in the sample cup X out of the first accommodating disc through the second manipulator;
s9: repeating S1, and placing a new empty sample cup Y in the sample cup placing position of the first accommodating disc;
s10: sequentially performing S3-S5, repeating (n+1) times, and completing filling, vibration mixing and incubation of the common reagent and the magnetic bead reagent in the second group of reagents;
s11: rotating the first accommodating disc to enable the sample cup Y to be transferred from the cup placing position to the transferring position, and transferring the sample cup Y to the transferring receiving position of the second accommodating disc through the second manipulator;
s12: rotating the second accommodating disc to enable the sample cup Y to enter a cleaning separation position from a transfer receiving position, and adsorbing magnetic beads mixed into a sample through a magnetic cleaning separator and cleaning at the same time;
s13: rotating the second accommodating disc to enable the sample cup Y to be transferred from the cleaning separation position to the reagent adding position, and adding the eluent on the rotating disc into the sample cup Y through the third pipetting needle;
s14: rotating the second accommodating disc to enable the sample cup Y to be rotated from the reagent adding position to the oscillation position for full oscillation;
s15: rotating the second accommodating disc to enable the sample cup to rotate out of the oscillation position, and carrying out heat preservation cultivation through the second incubation belt;
s16: and rotating the second accommodating disc to enable the sample cup Y to rotate to a sample outlet position, layering the sample and magnetic beads mixed in the sample under the action of the second magnetic separator, and transferring supernatant to the detection unit through the fourth pipetting needle to finish sample pretreatment.
One side of the first reaction component is provided with a sample cup recycling mechanism.
One sides of the first pipetting needle, the second pipetting needle, the third pipetting needle, the fourth pipetting needle and the fifth pipetting needle are provided with cleaning mechanisms.
One side of sample conveyer belt is provided with sample classification unit, sample classification unit is including manual advance appearance passageway, the categorised storage box of sample, manual recovery passageway that set up in order, manual advance appearance passageway, the categorised storage box of sample, manual one end of retrieving the passageway is provided with sample frame conveying passageway, the both sides of sample frame conveying passageway are provided with glass stand transport manipulator and sample cup transport manipulator respectively.
The detection unit is a liquid chromatography tandem mass spectrometry device, an immunoassay device or a biochemical analysis device.
According to the sample pretreatment method of the clinical automatic analyzer, the pretreatment unit supported by the method adopts a modularized design, and all modules are mutually independent and convenient to debug; the consistent pretreatment module is matched with the magnetic bead reagent containing the separable target objects, so that different pretreatment flows can be freely selected according to different target objects; the incubation reaction disc adopts a circle of reaction cup positions, so that the reaction cup can be transferred to any position through forward rotation/reverse rotation under the setting of a program, the reaction time is set freely, and more pretreatment flows can be applied under the condition that hardware is not changed; the reaction plate is integrated with the cleaning function, and can be connected with a liquid phase sample injection mechanism. The invention has wide application, is flexible and convenient, can ensure a high-flux automatic mode and improves the detection efficiency of the target object.
Drawings
Fig. 1 is a schematic structural view of a clinical automatic analyzer according to the present invention.
Detailed Description
The following describes embodiments of the present invention in detail with reference to the accompanying drawings, and the embodiments and specific working procedures of the present invention are given by implementing the present embodiment on the premise of the technical solution of the present invention, but the protection scope of the present invention is not limited to the following embodiments.
Example 1:
as shown in fig. 1, the clinical automatic analyzer according to the present invention is composed of three modules of a sample delivery unit 100, a pre-processing unit 200, and a detection unit 300.
Wherein the sample conveying unit 100 is used for conveying samples, and conveying sample tubes containing samples to be tested through the sample conveying belt 101; the sample conveying unit 100 can be abutted to an automatic pipeline, and a sample classifying unit 400 is further arranged on one side of the sample conveying belt 101 for processing emergency samples needing manual sample injection.
The sample classification unit 400 comprises a manual sample introduction channel 401, a sample classification storage box 402 and a manual recovery channel 403 which are sequentially arranged, wherein a sample rack conveying channel 404 is arranged at one end of the manual sample introduction channel 401, one end of the sample classification storage box 402 and one end of the manual recovery channel 403, a cup holder conveying manipulator 405 is arranged between the sample rack conveying channel 404 and the sample conveying belt 101, and a sample cup conveying manipulator 406 positioned between the manual sample introduction channel 401 and the sample classification storage box 402 is arranged at the other side of the sample rack conveying channel 404. The manual sample feeding channel 401 is used for storing a sample tube rack, a sample tube of the sample tube rack is filled with samples to be tested, the sample tube rack can be manually pushed to one end close to the sample rack conveying channel 404, then the samples tube rack is transferred to the sample rack conveying channel 404 through the cup holder conveying manipulator 405, then the sample tube rack is moved forward to the sample classification storage box 402 along the sample rack conveying channel 404, at the moment, the sample tube filled with the samples to be tested is transferred to the sample classification storage box 402 for classification storage through the sample cup conveying manipulator 406, the empty sample tube rack is still moved forward along the sample rack conveying channel 404 to the manual recovery channel 403, and is transferred to the manual recovery channel 403 through the cup holder conveying manipulator 405, and then is pulled out to the other end manually; the sample tubes in the sorting cassette 402 are transferred to the sample conveyor 101 by the cup holder transfer robot 405 at a proper time.
The pretreatment unit 200 is used for pretreating a sample, and can treat the required sample sequentially, in parallel and alternatively according to the purpose of experiments, and comprises a sample cup storage mechanism, a reagent storage mechanism, a reaction mechanism, a liquid transfer mechanism, a sample cup transfer mechanism and the like.
Specifically, the sample cup storage mechanism is a storage box 210 capable of storing a large amount of empty sample cups and automatically feeding, the reagent storage mechanism is a rotating disc 221 capable of being matched with the liquid transfer mechanism, a plurality of groups of reagent storage positions 222 are arranged on the rotating disc, each group of reagent storage positions 222 comprises a plurality of containing holes which are sequentially arranged along the radius of the rotating disc 221, common reagents and/or reagents containing magnetic beads can be placed according to experimental requirements, and the number and the positions of the common reagents and the reagents containing the magnetic beads are arranged according to practical conditions.
The reaction mechanism generally comprises a first reaction component and a second reaction component which are used in combination, wherein the first reaction component comprises a first accommodating disc 231 which is an annular disc rotating around the center, and a plurality of sample cup placing positions are uniformly distributed on the disc body; in order to achieve the purposes of cup placement, sample addition, reagent addition, mixing, separation and the like, a cup placement position P1, a sample addition position P2, a reagent addition position P3, a separation position P4 and a transfer position P5 are arranged on the running track of the first accommodating disc 231, and a first magnetic separator 232 (which can adopt a magnet and the like) is arranged at the separation position P4 and is arranged below the first accommodating disc 231 and used for adsorbing magnetic beads in a sample cup to separate the magnetic beads from liquid. A separate first oscillator 233 is installed near the first receiving tray 231, and is sufficiently oscillated to uniformly mix the sample and the reagent when the sample cup is transferred thereto. Meanwhile, in order to make all the sample cup placement positions have adjustable constant temperature environments, the samples therein are always in an incubation state, and a fixedly arranged first incubation belt 234 is correspondingly arranged below the first accommodating tray 231.
The second reaction component is similar to the first reaction component in structure, and comprises an annular second accommodating disc 241 uniformly provided with a plurality of sample cup accommodating positions, the second accommodating disc 241 rotates around the center, and a first incubation belt 242 capable of keeping a constant temperature environment is fixedly arranged below the second accommodating disc 241; in order to achieve the purposes of adding reagent, mixing, separating and the like, a transfer receiving position C1, a reagent adding position C2, an oscillating position C3, a cleaning separating position C4 and a sample outputting position C5 are arranged on the moving track of the second accommodating disc 241. A second oscillator 243 is installed at the oscillation position C3 for uniformly mixing the sample and the reagent. A magnetic washing separator 244 is installed at the washing separation position C4, and is installed at one side of the second accommodating tray 241, and includes a magnetic adsorption mechanism for adsorbing magnetic beads in a reagent, a liquid injection mechanism for washing the magnetic beads, and a liquid discharge mechanism. The sample outlet position C5 is provided with a second magnetic separator 245 (such as a magnet) which is arranged below the second accommodating disc 241 and is used for adsorbing magnetic beads in the reagent to separate the magnetic beads from the sample and the reagent.
In the detection process, a sample cup is required to be used for containing a sample and a reagent, so that a sample cup transfer mechanism is required to be provided for transferring the sample cup. Specifically, the sample cup transfer mechanism includes a first robot 251 disposed near the cup placement position P1 of the storage cassette 210, the first oscillator 233, and the first accommodating tray 231, and a second robot 252 between the transfer position P5 of the first accommodating tray 231 and the transfer receiving position C1 of the second accommodating tray 241.
In order to fill a sample or a reagent, it is necessary to provide a liquid transfer mechanism including a first pipetting needle 261 mounted between the sample filling position P2 of the first housing tray 231 and the sample conveyer 101, a second pipetting needle 262 mounted between the rotating tray 221 and the reagent filling position P3 of the first housing tray 231, a third pipetting needle 263 mounted between the rotating tray 221 and the reagent filling position C2 of the second housing tray 241, and a fourth pipetting needle 264 mounted between the sample filling position P4 of the detecting unit 300 and the separating position P4 of the first housing tray 231 and the sample discharging position C5 of the second housing tray 241. In the case of replacing the sample cup, a liquid temporary storage mechanism and a sample cup recovery mechanism are further provided on one side of the first accommodating tray 231, wherein the liquid temporary storage mechanism is a fifth pipetting needle 271 located near the separation position P4, and the sample cup recovery mechanism is a recovery box 281 installed at any point on the movement track of the second manipulator 252. In order to avoid cross contamination, a cleaning mechanism is arranged on one side of a pipetting needle used by the liquid transferring mechanism and the liquid temporary storage mechanism, and a cleaning operation is performed once every time the pipetting needle is used.
The detection unit 300 is used for detecting the sample processed by the preprocessing unit 200, and is a liquid chromatography tandem mass spectrometry device, an immunoassay device, a biochemical analysis device, etc., for realizing diversified analyses.
When in use, there are two typical cases:
1. the sample is pretreated by using reagents R1, R2, R3, R4 and R5, wherein R1, R2 and R3 are common reagents, R4 is a reagent containing magnetic beads, and R5 is an eluent. The sample is incubated with the addition of reagents R1, R2, R3, and R4, and the resulting target is attached to magnetic beads, requiring detection analysis after elution and separation.
The above-mentioned reagents R1, R2, R3, R4, R5 are placed on the reagent placement sites 222 of one group of the rotating disk 221, and when the number of reagents is large, the reagents may be placed in two or more groups. The specific pretreatment steps are as follows:
s1: the first manipulator 251 takes an empty sample cup X from the storage box 210 and places it in the sample cup placement position of the first accommodating tray 231, and at this time, the sample cup X is located in the cup placement position P1;
s2: rotating the first containing plate 231 to rotate the sample cup X from the cup placing position P1 to the sample adding position P2, and injecting one sample on the sample conveying belt 101 into the sample cup X through the first pipetting needle 261;
s3: rotating the first accommodating disc 231 to enable the sample cup X to be transferred from the sample adding position P2 to the reagent adding position P3, and filling the reagent R1 on the rotating disc 221 into the sample cup X through the second pipetting needle 262;
s4: rotating the first accommodating disc 231 to enable the sample cup X to be transferred from the reagent adding position P3 to the cup placing position P1, and transferring the sample cup X into the first oscillator 233 through the first manipulator 251 to perform full oscillation;
s5: the first manipulator 251 moves the sample cup X back to the cup placing position P1, and the first incubation belt 234 is adjusted to 37 ℃ for incubation;
s6: repeating S3-S5, filling a reagent R2 into the sample cup X, and carrying out shaking mixing and heat preservation cultivation;
s7: repeating the step S6 for two times to finish filling, shaking and mixing and heat-preserving cultivation of the reagent R3 and the reagent R4;
s8: rotating the first accommodating tray 231 to transfer the sample cup X from the cup accommodating position P1 to the transfer position P5, and transferring the sample cup X into the sample cup accommodating position of the second accommodating tray 241 by the second manipulator 252, wherein the sample cup X is located at the transfer receiving position C1;
s9: rotating the second accommodating tray 241 to enable the sample cup X to enter the cleaning separation position C4 from the transfer receiving position C1, and adsorbing the magnetic beads mixed into the sample by the magnetic cleaning separator 244 and cleaning the same;
s10: rotating the second accommodating disc 241 to enable the sample cup X to be transferred from the cleaning separation position C4 to the reagent adding position C2, and filling the reagent R5 on the rotating disc 221 into the sample cup X through the third pipetting needle 263;
s11: rotating the second accommodating disc 241 to enable the sample cup X to be rotated from the reagent adding position C2 to the oscillation position C3 for full oscillation;
s12: rotating the second accommodating plate 241 to enable the sample cup X to rotate out of the oscillation position C3, and then adjusting the second incubation belt 242 to 37 ℃ for incubation;
s13: the second accommodation plate 241 is rotated to rotate the sample cup X to the sample outlet position C5, and the sample and the magnetic beads mixed therein are layered by the second magnetic separator 245, and the supernatant is transferred to the detection unit 300 through the fourth pipetting needle 264.
2. Pretreating a sample by using two groups of reagents, wherein the first group comprises reagents R1, R2 and R4, wherein R1 and R2 are common reagents, and R4 is a reagent comprising a precipitator and magnetic beads; the second group comprises reagents R33, R44 and R55, wherein R33 is a common reagent, R44 is a reagent comprising magnetic beads, and R55 is an eluent. Two sets of reagents are correspondingly placed on two adjacent sets of reagent placement sites 222 on the rotating disc 221.
When pretreatment is carried out, firstly, a sample is injected into a sample cup X, reagents R1, R2 and R4 are added for incubation culture, then the sample and magnetic beads are separated, a supernatant containing a target object is transferred into a new sample cup Y, the reagents R33 and R44 are continuously added for incubation culture, and the separated target object is attached to the magnetic beads and needs to be detected and analyzed after elution and separation. The specific pretreatment steps are as follows:
s1: the first manipulator 251 takes an empty sample cup X from the storage box 210 and places it in the sample cup placement position of the first accommodating tray 231, and at this time, the sample cup X is located in the cup placement position P1;
s2: rotating the first containing plate 231 to rotate the sample cup X from the cup placing position P1 to the sample adding position P2, and injecting one sample on the sample conveying belt 101 into the sample cup X through the first pipetting needle 261;
s3: rotating the first accommodating disc 231 to enable the sample cup X to be transferred from the sample adding position P2 to the reagent adding position P3, and filling the reagent R1 on the rotating disc 221 into the sample cup X through the second pipetting needle 262;
s4: rotating the first accommodating disc 231 to enable the sample cup X to be transferred from the reagent adding position P3 to the cup placing position P1, and transferring the sample cup X into the first oscillator 233 through the first manipulator 251 to perform full oscillation;
s5: the first manipulator 251 moves the sample cup X back to the cup placing position P1, and the first incubation belt 234 is adjusted to 37 ℃ for incubation;
s6: repeating S3-S5, filling a reagent R2 into the sample cup X, and carrying out shaking mixing and heat preservation cultivation;
s7: repeating the step S6, and finishing filling, vibration mixing and heat preservation cultivation of the reagent R4;
s8: rotating the first accommodating disk 231 to enable the sample cup X to be rotated from a cup placing position P1 to a separating position P4, separating the sample from the magnetic beads under the action of the first magnetic attraction separator 232, and sucking all supernatant liquid through the fifth pipetting needle 271;
s9: rotating the first accommodating tray 231 to transfer the sample cup X with the magnetic beads from the separation position P4 to the transfer position P5, and transferring the sample cup X and the magnetic beads therein into the recovery box 281 by the second manipulator 252;
s10: repeating S1, placing a new empty sample cup Y in the sample cup placement position of the first receiving tray 231 at the cup placement position P1;
s11: repeating S6 for two times, and sequentially completing filling, shaking and mixing and heat preservation cultivation of the reagent R33 and the reagent R44;
s12: rotating the first accommodating tray 231 to transfer the sample cup Y from the cup accommodating position P1 to the transfer position P5, and transferring the sample cup Y into the sample cup accommodating position of the second accommodating tray 241 by the second manipulator 252, wherein the sample cup Y is located at the transfer receiving position C1;
s13: rotating the second accommodating tray 241 to enable the sample cup Y to enter a cleaning and separating position C4 from a transferring and receiving position C1, and adsorbing magnetic beads mixed into a sample through a magnetic cleaning and separating device 244 and cleaning at the same time;
s14: rotating the second accommodating disc 241 to enable the sample cup Y to be transferred from the cleaning separation position C4 to the reagent adding position C2, and filling the reagent R55 on the rotating disc 221 into the sample cup Y through the third pipetting needle 263;
s15: rotating the second accommodating disc 241 to enable the sample cup Y to be rotated from the reagent adding position C2 to the oscillation position C3 for full oscillation;
s16: rotating the second accommodating plate 241 to enable the sample cup X to rotate out of the oscillation position C3, and then adjusting the second incubation belt 242 to 37 ℃ for incubation;
s17: the second accommodation plate 241 is rotated to make the sample cup Y rotate to the sample outlet position C5, and the sample and the magnetic beads mixed therein are layered under the action of the second magnetic separator 245, and the supernatant is transferred to the detection unit 300 through the fourth pipetting needle 264.
Example 2:
the sample pretreatment method of the clinical automatic analyzer comprises three modules of a sample conveying unit 100, a pretreatment unit 200 and a detection unit 300. Compared to example 1, only the liquid temporary storage mechanism, the second reaction module, and the engagement mechanism between the second reaction module and the first reaction module and the sample cup storage mechanism in the pretreatment unit 200 are reduced, and the rest is the same as in example 1. It is mainly used in the case that after separating magnetic beads from a sample, detection and analysis are required for the supernatant containing the target object.
Specifically, a sample is subjected to pretreatment by using a group of reagents R1, R2 and R4, wherein R1 and R2 are common reagents, and R4 is a reagent containing a precipitant and magnetic beads. The operation steps are as follows:
s1: the first manipulator 251 takes an empty sample cup X from the storage box 210 and places it in the sample cup placement position of the first accommodating tray 231, and at this time, the sample cup X is located in the cup placement position P1;
s2: rotating the first containing plate 231 to rotate the sample cup X from the cup placing position P1 to the sample adding position P2, and injecting one sample on the sample conveying belt 101 into the sample cup X through the first pipetting needle 261;
s3: rotating the first accommodating disc 231 to enable the sample cup X to be transferred from the sample adding position P2 to the reagent adding position P3, and filling the reagent R1 on the rotating disc 221 into the sample cup X through the second pipetting needle 262;
s4: rotating the first accommodating disc 231 to enable the sample cup X to be transferred from the reagent adding position P3 to the cup placing position P1, and transferring the sample cup X into the first oscillator 233 through the first manipulator 251 to perform full oscillation;
s5: the first manipulator 251 moves the sample cup X back to the cup placing position P1, and the first incubation belt 234 is adjusted to 37 ℃ for incubation;
s6: repeating S3-S5, filling a reagent R2 into the sample cup X, and carrying out shaking mixing and heat preservation cultivation;
s7: repeating the step S6, and finishing filling, vibration mixing and heat preservation cultivation of the reagent R4;
s8: the first receiving tray 231 is rotated to transfer the sample cup X from the cup placement position P1 to the separation position P4, the sample and the magnetic beads are separated by the first magnetic separator 232, and the supernatant is transferred to the detection unit 300 by the fourth pipetting needle 264.
It should be noted that, in the description of the present invention, terms such as "front", "rear", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element to be referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
Claims (5)
1. A sample pretreatment method of a clinical automatic analyzer is characterized in that:
the clinical automatic analyzer comprises a sample conveying unit, a preprocessing unit and a detection unit which are sequentially connected, wherein the preprocessing unit comprises:
a first reaction assembly, comprising:
the first accommodating disc is an annular disc rotating around the center, and a plurality of sample cup placing positions are arranged on the first accommodating disc;
a first incubation zone disposed below the first receiving tray for maintaining a sample located on the first receiving tray in a constant temperature state;
the first oscillator is arranged below the first accommodating disc or outside the first accommodating disc and is used for uniformly mixing the sample and the reagent; and
the first magnetic separator is arranged below the first accommodating disc and is used for adsorbing magnetic beads in the reagent to separate the magnetic beads from the sample and the reagent;
the second reaction subassembly sets up in first reaction subassembly one side, the second reaction subassembly includes:
the second accommodating disc is an annular disc rotating around the center, and a plurality of sample cup placing positions are arranged on the second accommodating disc;
a second incubation zone disposed below the second holding tray for maintaining the samples on the second holding tray in a constant temperature state;
the second oscillator is arranged below the second accommodating disc or outside the second accommodating disc and is used for uniformly mixing the sample and the reagent;
the second magnetic separator is arranged below the second accommodating disc and is used for adsorbing magnetic beads in the reagent to separate the magnetic beads from the sample and the reagent; and
the magnetic washing separator is arranged at one side of the second accommodating disc and comprises an adsorption mechanism for adsorbing magnetic beads in the reagent, a liquid injection mechanism for washing the magnetic beads and a liquid discharge mechanism;
the reagent storage mechanism comprises a rotating disc, wherein a plurality of groups of reagent placement positions are arranged on the rotating disc, and each group of reagent placement positions comprises a plurality of containing holes which are sequentially arranged along the radius of the rotating disc;
the first manipulator is arranged on one side of the first accommodating disc and is used for feeding empty sample cups into a cup placing position of the first accommodating disc and transferring the sample cups between the cup placing position and the first oscillator;
the second manipulator is arranged between the first accommodating disc and the second accommodating disc and is used for transferring the sample cup from the transfer position of the first accommodating disc to the transfer receiving position of the second accommodating disc;
the first pipetting needle is arranged between the first accommodating disc and the sample conveying unit, and the overlapping part of the first pipetting needle and the first accommodating disc forms a sample adding position;
the second pipetting needle is arranged between the rotating disc and the first accommodating disc, and the overlapping part of the second pipetting needle and the first accommodating disc forms a reagent adding position;
the third pipetting needle is arranged between the rotating disc and the second accommodating disc, and the overlapping part of the third pipetting needle and the second accommodating disc forms another reagent adding position;
the fourth pipetting needle is arranged between the first accommodating disc, the second accommodating disc and the detection unit, the overlapping part of the fourth pipetting needle and the first accommodating disc forms a separation position, the overlapping part of the fourth pipetting needle and the second accommodating disc forms a sample outlet position, and the fourth pipetting needle is used for transferring samples from the first accommodating disc or the second accommodating disc to the detection unit;
the fifth pipetting needle is arranged on one side of the first accommodating disc and overlapped with the first accommodating disc in the cup placing position;
the first magnetic separator is arranged corresponding to the separation position, and the second magnetic separator is arranged corresponding to the sample outlet position;
for the case of adding n (n is more than or equal to 1) common reagents and containing precipitants and magnetic bead reagents into a sample, the sample pretreatment method comprises the following steps:
s1: taking an empty sample cup by a first manipulator and placing the empty sample cup at a cup placing position of a first accommodating disc;
s2: rotating the first accommodating disc to enable the sample cup to be rotated from a cup placing position to a sample adding position, and adding one sample on the sample conveying belt into the sample cup through the first pipetting needle;
s3: rotating the first accommodating disc to enable the sample cup to be transferred from the sample adding position to the reagent adding position, and filling the first common reagent on the rotating disc into the sample cup through the second pipetting needle;
s4: rotating the first accommodating disc to enable the sample cup to be transferred from the reagent adding position to the cup placing position, and transferring the sample cup into the first oscillator through the first manipulator to perform full oscillation;
s5: the sample cup is moved back to the cup position by a first manipulator, and heat preservation cultivation is carried out by a first incubation belt;
s6: sequentially carrying out S3-S5, repeating n times to finish filling, shaking and mixing and heat preservation cultivation of other common reagents and reagents containing precipitants and magnetic beads;
s7: rotating the first accommodating disc to enable the sample cup to be rotated from a cup placing position to a separating position, separating the sample from the magnetic beads under the action of the first magnetic attraction separator, and transferring supernatant to the detection unit through the fourth pipetting needle to finish sample pretreatment;
for the case of adding n (n is more than or equal to 1) common reagents, magnetic bead reagents and eluents into a sample, the sample pretreatment method comprises the following steps:
s1: taking an empty sample cup by a first manipulator and placing the empty sample cup at a cup placing position of a first accommodating disc;
s2: rotating the first accommodating disc to enable the sample cup to be rotated from a cup placing position to a sample adding position, and adding one sample on the sample conveying belt into the sample cup through the first pipetting needle;
s3: rotating the first accommodating disc to enable the sample cup to be transferred from the sample adding position to the reagent adding position, and filling the first common reagent on the rotating disc into the sample cup through the second pipetting needle;
s4: rotating the first accommodating disc to enable the sample cup to be transferred from the reagent adding position to the cup placing position, and transferring the sample cup into the first oscillator through the first manipulator to perform full oscillation;
s5: the sample cup is moved back to the cup position by a first manipulator, and heat preservation cultivation is carried out by a first incubation belt;
s6: sequentially carrying out S3-S5, repeating n times, and completing filling, vibration mixing and heat preservation cultivation of other common reagents and magnetic bead reagents;
s7: rotating the first accommodating disc to enable the sample cup to be transferred from the cup placing position to the transferring position, and transferring the sample cup to the transferring receiving position of the second accommodating disc through the second manipulator;
s8: rotating the second accommodating disc to enable the sample cup to enter the cleaning separation position from the transfer receiving position, and adsorbing magnetic beads mixed into the sample through the magnetic cleaning separator, and cleaning at the same time;
s9: rotating the second accommodating disc to enable the sample cup to be transferred from the cleaning separation position to the reagent adding position, and adding the eluent on the rotating disc into the sample cup through the third pipetting needle;
s10: rotating the second accommodating disc to enable the sample cup to be rotated from the reagent adding position to the oscillation position for full oscillation;
s11: rotating the second accommodating disc to enable the sample cup to rotate out of the oscillation position, and carrying out heat preservation cultivation through the second incubation belt;
s12: rotating the second containing disc to enable the sample cup to rotate to a sample outlet position, layering the sample and magnetic beads mixed in the sample under the action of the second magnetic separator, and transferring supernatant to the detection unit through the fourth pipetting needle to finish sample pretreatment;
aiming at the condition that two groups of reagents are added into a sample, wherein the first group of reagents are m (m is more than or equal to 1) common reagents and contain precipitants, and the second group of reagents are n (n is more than or equal to 1) common reagents, magnetic bead reagents and eluents, the sample pretreatment method comprises the following steps:
s1: taking an empty sample cup X by a first manipulator and placing the empty sample cup X in a cup placing position of a first accommodating disc;
s2: rotating the first accommodating disc to enable the sample cup X to be transferred from the cup placing position to the sample adding position, and adding one sample on the sample conveying belt into the sample cup X through the first pipetting needle;
s3: rotating the first accommodating disc to enable the sample cup X to be transferred from the sample adding position to the reagent adding position, and filling a first common reagent of a first group of reagents on the rotating disc into the sample cup X through the second pipetting needle;
s4: rotating the first accommodating disc to enable the sample cup X to be transferred from the reagent adding position to the cup placing position, and transferring the sample cup X into the first oscillator through the first manipulator to perform full oscillation;
s5: moving the sample cup X back to the cup placing position by a first manipulator, and carrying out heat preservation cultivation by a first incubation belt;
s6: sequentially performing S3-S5, repeating m times to finish the filling, shaking and mixing and heat preservation cultivation of the rest common reagents of the first group and the reagents containing the precipitant and the magnetic beads;
s7: rotating the first accommodating disc to enable the sample cup X to be rotated from a cup placing position to a separating position, separating the sample from the magnetic beads under the action of the first magnetic attraction separator, and then sucking all supernatant through the fifth pipetting needle;
s8: rotating the first accommodating disc, transferring the sample cup X with the magnetic beads from the separation position to the rotation position, and moving the sample cup X and the magnetic beads in the sample cup X out of the first accommodating disc through the second manipulator;
s9: repeating S1, and placing a new empty sample cup Y in the sample cup placing position of the first accommodating disc;
s10: sequentially performing S3-S5, repeating (n+1) times, and completing filling, vibration mixing and incubation of the common reagent and the magnetic bead reagent in the second group of reagents;
s11: rotating the first accommodating disc to enable the sample cup Y to be transferred from the cup placing position to the transferring position, and transferring the sample cup Y to the transferring receiving position of the second accommodating disc through the second manipulator;
s12: rotating the second accommodating disc to enable the sample cup Y to enter a cleaning separation position from a transfer receiving position, and adsorbing magnetic beads mixed into a sample through a magnetic cleaning separator and cleaning at the same time;
s13: rotating the second accommodating disc to enable the sample cup Y to be transferred from the cleaning separation position to the reagent adding position, and adding the eluent on the rotating disc into the sample cup Y through the third pipetting needle;
s14: rotating the second accommodating disc to enable the sample cup Y to be rotated from the reagent adding position to the oscillation position for full oscillation;
s15: rotating the second accommodating disc to enable the sample cup to rotate out of the oscillation position, and carrying out heat preservation cultivation through the second incubation belt;
s16: and rotating the second accommodating disc to enable the sample cup Y to rotate to a sample outlet position, layering the sample and magnetic beads mixed in the sample under the action of the second magnetic separator, and transferring supernatant to the detection unit through the fourth pipetting needle to finish sample pretreatment.
2. The method for sample pretreatment of a clinical automatic analyzer according to claim 1, wherein: one side of the first reaction component is provided with a sample cup recycling mechanism.
3. The method for sample pretreatment of a clinical automatic analyzer according to claim 1, wherein: one sides of the first pipetting needle, the second pipetting needle, the third pipetting needle, the fourth pipetting needle and the fifth pipetting needle are provided with cleaning mechanisms.
4. The method for sample pretreatment of a clinical automatic analyzer according to claim 1, wherein: one side of sample conveyer belt is provided with sample classification unit, sample classification unit is including manual advance appearance passageway, the categorised storage box of sample, manual recovery passageway that set up in order, manual advance appearance passageway, the categorised storage box of sample, manual one end of retrieving the passageway is provided with sample frame conveying passageway, the both sides of sample frame conveying passageway are provided with glass stand transport manipulator and sample cup transport manipulator respectively.
5. The method for sample pretreatment of a clinical automatic analyzer according to claim 1, wherein: the detection unit is a liquid chromatography tandem mass spectrometry device, an immunoassay device or a biochemical analysis device.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104714042A (en) * | 2013-12-16 | 2015-06-17 | 深圳市亚辉龙生物科技有限公司 | Full-automatic chemiluminescence immune analyzer and use method thereof |
| WO2017184244A1 (en) * | 2016-04-22 | 2017-10-26 | Becton. Dickinson And Company | Automated diagnostic analyzer and method for its operation |
| CN112946133A (en) * | 2021-02-04 | 2021-06-11 | 科诺美(北京)科技有限公司 | Application method of biological sample pretreatment system |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104714042A (en) * | 2013-12-16 | 2015-06-17 | 深圳市亚辉龙生物科技有限公司 | Full-automatic chemiluminescence immune analyzer and use method thereof |
| WO2017184244A1 (en) * | 2016-04-22 | 2017-10-26 | Becton. Dickinson And Company | Automated diagnostic analyzer and method for its operation |
| CN112946133A (en) * | 2021-02-04 | 2021-06-11 | 科诺美(北京)科技有限公司 | Application method of biological sample pretreatment system |
Non-Patent Citations (1)
| Title |
|---|
| 全自动生化分析仪交叉污染的测试与评估;王志伟;蔡永梅;毛东英;刘京;;中国医学装备(第06期);45-48 * |
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