CN113391439A - Color-related microscope imaging system and control method thereof - Google Patents
Color-related microscope imaging system and control method thereof Download PDFInfo
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Abstract
The invention discloses a color dependent microscope imaging system. The carrying platform is used for placing a target object; the color-variable luminous source is used for providing color-variable light to the target object; the user input interface provides a user to input three color ranges and three color adjustment values of the variable color light; the control unit adjusts the variable color light of the variable color light source according to the three color ranges and the three color adjustment values input by the user; and the imaging unit is used for shooting the target object; when the control module adjusts the color-changeable light of the color-changeable light source according to the three color ranges and the three color adjustment values, the imaging unit captures a plurality of images of the color-changeable light under different color values. The invention has the advantages that: the clearest images of the variable color light with different color values are displayed on a screen after being spliced, so that interested areas can be conveniently researched.
Description
Technical Field
The invention relates to the technical field of picture imaging, in particular to an imaging system based on a microscope shooting picture and a control method thereof.
Background
The statements in this section merely provide background information related to the present disclosure and may not constitute prior art.
The microscope is used for researching images of various organisms or cells in a micro state, has wide application range, and is the most important instrument in the fields of biology and medicine. The microscope is a tool for observing microscopic objects, the field of view of the microscope is very limited, only a small area can be observed at the same time, and for a relatively large object or a slightly large area, the whole appearance of the object cannot be directly obtained from the microscope, so that the microscope needs to be continuously adjusted to obtain images of different areas, but the panoramic observation in one field of view cannot be usually realized.
In addition, since the microscope is a technique that requires light to see the inside of an object, and the variable color light suitable for each object is different in practice, it is difficult to directly obtain a clear photograph due to the structure of the conventional microscope itself. Without a clear figure, all subsequent studies became meaningless.
It should be noted that the above background description is only for the sake of clarity and complete description of the technical solutions of the present invention and for the understanding of those skilled in the art. Such solutions are not considered to be known to the person skilled in the art merely because they have been set forth in the background section of the invention.
Disclosure of Invention
In order to overcome the defects in the prior art, embodiments of the present invention provide a color-dependent microscope imaging system and a control method thereof, where the color-dependent microscope imaging system is capable of viewing the texture and the overall view of different regions of an object under study in a panoramic manner under variable color lights of different colors.
The embodiment of the application discloses: a color-dependent microscope imaging system includes a stage, a variable color light source, a user input interface, a control unit, and an imaging unit. The object carrying platform is used for placing a target object to be observed; the color-changeable luminous source is positioned above the object stage and used for providing color-changeable light for the object; the user input interface is used for providing a user to input three color ranges and three color adjusting values of the variable color light; the control unit is coupled to the variable color light source and the user input interface, and is configured to adjust the variable color light of the variable color light source according to the three color ranges and the three color adjustment values input by the user; the imaging unit is used for shooting the target object so as to generate a plurality of images in each second; when the control module adjusts the color-changeable light of the color-changeable light source according to the three color ranges and the three color adjustment values, the imaging unit captures a plurality of images of the color-changeable light under different color values.
Further, the three color ranges include three maximum color values and three minimum color values, and the control unit adjusts the variable color light of the variable color light emitting source to the three maximum color values first, and adjusts the variable color light of the variable color light emitting source in a decreasing manner according to one of the three color adjustment values after every predetermined time interval until the variable color light of the variable color light emitting source is adjusted to the three minimum color values.
Further, the color-dependent microscope imaging system further comprises: a calculating unit, coupled to the imaging unit, for calculating the sharpness of the plurality of images; and a determining unit, coupled to the calculating unit, for determining a clearest image from the plurality of images.
Further, the imaging unit divides the target object into mxn regions, and takes p block images for each region, wherein the p block images for each region are captured under the same color value of the variable color light.
Further, the determining unit firstly defaults a first image as a main image to be finally output, then calculates the definition of p block images for each region, and if the definition of a specific block image is greater than the highest definition of the region on the main image, updates the highest definition value, and simultaneously replaces the default block image of the region on the main image with the specific block image.
Further, the calculating unit calculates the sharpness of the plurality of images using a first function, which is expressed by:
D(f)＝∑y∑x|f(x+2，y)-f(x，y)|2 (1)；
in the formula: f (x, y) represents the gray value of the pixel point (x, y) corresponding to the image f, and D (f) is the image definition calculation result.
Further, the calculating unit calculates the sharpness of the plurality of images using a second function, which is expressed by:
D(f)＝∑y∑x(|f(x+1，y)-f(x，y)|2+|f(x，y+1)-f(x，y)|2) (2)；
in the formula: f (x, y) represents the gray value of the pixel point (x, y) corresponding to the image f, and D (f) is the image definition calculation result.
Further, the calculating unit calculates the sharpness of the plurality of images using a third function, which is expressed by:
D(f)＝∑y∑x(|f(x，y)-f(x，y-1)|+|f(x，y)-f(x+1，y)|) (3)；
in the formula: f (x, y) represents the gray value of the pixel point (x, y) corresponding to the image f, and D (f) is the image definition calculation result.
Further, the calculation unit calculates the sharpness of the block image corresponding to a first group of regions using the first function, calculates the sharpness of the block image corresponding to a second group of regions using the second function, and calculates the sharpness of the block image corresponding to a third group of regions using the third function.
The embodiment of the application discloses: a method of controlling a color-dependent microscopy imaging system including a variable color light source for providing a variable color light to an object and an imaging unit for capturing the object to produce a plurality of images per second. The method comprises the following steps: providing a user input interface for inputting three color ranges and three color adjustment values of the variable color light to a user; adjusting the variable color light of the variable color light source according to the three color ranges and the three color adjustment values input by the user; and controlling the imaging unit to capture a plurality of images of the variable color light under different color values when the variable color light of the variable color light source is adjusted according to the three color ranges and the three color adjustment values.
By means of the technical scheme, the invention has the following beneficial effects: the clearest images of different variable color lights shot in the microscope visual field are displayed on a screen after being spliced, so that the overall view of a researched object in the microscopic visual field can be conveniently checked, and an interested area can be conveniently researched.
In order to make the aforementioned and other objects, features and advantages of the invention comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view of a color-correlated microscopy imaging system in a first embodiment of the invention.
FIG. 2 is a block diagram of a color-correlated microscopy imaging system according to a first embodiment of the present invention.
FIG. 3 is a block diagram of a color-correlated microscopy imaging system according to a second embodiment of the present invention.
Fig. 4 is a schematic view of an image taken by the imaging unit.
Fig. 5 is a flow chart of a method of controlling a color dependent microscope imaging system in an embodiment of the invention.
Reference numerals of the above figures: 10. 30, a color-dependent microscope imaging system; 110. a carrier platform; 120. a variable color light emitting source; 130. a user input interface; 140. a control unit; 150. an imaging unit; 360. a calculation unit; 370. a determination unit; OB1, target; r _ RANGE, G _ RANGE, B _ RANGE, three color RANGEs; Δ R, Δ G, Δ B, three color adjustment values; r _ MAX, G _ MAX, B _ MAX, three maximum color values; r _ MIN, G _ MIN, B _ MIN, three minimum color values; AREA 11-AREA 35, AREA; s510, S520, S530 and step.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that, in the description of the present invention, the terms "first", "second", and the like are used for descriptive purposes only and for distinguishing similar objects, and no precedence between the two is considered as indicating or implying relative importance. In addition, in the description of the present invention, "a plurality" means two or more unless otherwise specified.
Please refer to fig. 1 and fig. 2. Fig. 1 is a schematic diagram of a color-dependent microscopy imaging system 10 according to a first embodiment of the present invention, and fig. 2 is a block diagram of a color-dependent microscopy imaging system 10 according to a first embodiment of the present invention. As shown in fig. 1 and 2, a color-dependent microscope imaging system 10 includes a stage 110, a color-variable light source 120, a user input interface 130, a control unit 140, and an imaging unit 150. The object stage 110 is used for placing an object OB1 to be observed; the color-changeable light-emitting source 120 is located above the object stage 110 and is used for providing a color-changeable light to the object OB 1. The user input interface 130 is used for providing a user with three color RANGEs R _ RANGE, G _ RANGE, B _ RANGE and three color adjustment values Δ R, Δ G, Δ B for inputting the variable color light, wherein the three color RANGEs R _ RANGE, G _ RANGE, B _ RANGE respectively include three maximum color values R _ MAX, G _ MAX, B _ MAX and three minimum color values R _ MIN, G _ MIN, B _ MIN. The control unit 140 is coupled to the variable color light emitting sources 120 and the user input interface 130, and configured to adjust the variable color light emitting sources 120 according to the three color RANGEs R _ RANGE, G _ RANGE, and B _ RANGE input by the user and the three color adjustment values Δ R, Δ G, and Δ B. The imaging unit 150 is used to capture the object OB1 to generate a plurality of images per second. When the control unit 140 adjusts the variable color light of the variable color light source 120 according to the three color RANGEs R _ RANGE, G _ RANGE, B _ RANGE and the three color adjustment values Δ R, Δ G, Δ B, the imaging unit 150 captures a plurality of images of the variable color light at different color values, which is only an example and is not a limitation of the present invention.
In one possible embodiment, the variable color light emitting source 120 may comprise a multi-color LED light source, and the multi-color LED light source comprises at least R, G, B LED lamps, and the modulation frequency of the variable color light emitting source 120 is 40 kHz.
In one possible embodiment, the control unit 140 first adjusts the color-changeable light of the light-emitting source 120 into three maximum color values R _ MAX, G _ MAX, and B _ MAX, and then gradually decreases the color-changeable light of the light-emitting source 120 according to one of the three color adjustment values after every predetermined time interval until the color-changeable light of the light-emitting source 120 is adjusted into three minimum color values R _ MIN, G _ MIN, and B _ MIN. For example, the user can set all three maximum color values of the three color RANGEs R _ RANGE, G _ RANGE, and B _ RANGE of the variable color light to 255, all three minimum color values to 0, and all three color adjustment values Δ R, Δ G, and Δ B to 255, so that the control unit 140 first adjusts the color values (R, G, B) of the variable color light source 120 to (255 ), and at this time, the imaging unit 150 captures a plurality of images of the target object OB1 corresponding to the color values (R, G, B) of the variable color light to (255 ); then, after every predetermined time interval (for example, every 30 seconds or after the imaging unit 150 has captured all the images of the object OB 1), the control unit 140 first decrements one color value of the color-changeable light source 120 by 255 (three color adjustment values Δ R, Δ G, Δ B) each time, and sequentially decrements and adjusts the color value to (0,255,255), (255,0,255), (255, 0), (0,0,255), (0,255,0), (255,0,0, 0), (0,0,0) until all three color values of the color-changeable light source 120 are adjusted to 0 (three minimum color values R _ MIN, G _ MIN, B _ MIN). At this time, the imaging unit 150 also captures a plurality of images of the object OB1 corresponding to the variable color light (0,255,255), (255,0,255), (255, 0), (0,0,255), (0,255,0), (255,0,0), (0,0, 0). The three maximum color values, the three minimum color values, the three color adjustment values and the predetermined time interval are only exemplary and are not limitations of the present invention. The setting values can be designed into different values according to actual requirements, and the scope of the invention is also covered by the invention.
It is to be noted that the color adjustment values Δ R, Δ G, Δ B of only one of the color values can be decremented at a time when the color change is performed. Generally, when the color value (R, G, B) of the variable color light is (255,255,255), the corresponding color is white, and all three LED lamps (R _ LED, G _ LED, B _ LED) emit light together; when the color value (R, G, B) of the variable color light is (0,255,255), the corresponding color is cyan, and the two LED lamps (G _ LED and B _ LED) emit light together; when the color value (R, G, B) of the variable color light is (255,0,255), the corresponding color is magenta, and the two LED lamps (R _ LED, B _ LED) emit light together; when the color value (R, G, B) of the variable color light is (255, 0), the corresponding color is yellow, and the two LED lamps (R _ LED, G _ LED) emit light together; when the color value (R, G, B) of the variable color light is (0, 255), the corresponding color is blue, and only one LED lamp (B _ LED) emits light together; when the color value (R, G, B) of the variable color light is (0,255,0), the corresponding color is green, and only one LED lamp (G _ LED) emits light together; when the color value (R, G, B) of the variable color light is (255,0,0), the corresponding color is red, and only one LED lamp (R _ LED) emits light together; when the color value (R, G, B) of the variable color light is (0,0,0), the corresponding color is black, and all the LED lamps do not emit light at this time.
Referring to fig. 3, fig. 3 is a block diagram of a color-correlated microscope imaging system 30 according to a second embodiment of the present invention. The color-correlated microscopy imaging system 30 of fig. 3 is similar to the color-correlated microscopy imaging system 10 of fig. 2, except that the color-correlated microscopy imaging system 30 further comprises a calculating unit 360 and a determining unit 370. A calculating unit 360 is coupled to the imaging unit 150 for calculating the sharpness of the plurality of images. The determining unit 370 is coupled to the calculating unit 360 for determining a clearest image from the plurality of images.
Referring to fig. 4, fig. 4 is a schematic diagram of an image captured by the imaging unit 150. As shown in fig. 3, the imaging unit 150 divides the object OB1 into mxn regions (e.g., 3x5 regions AREA 11-AREA 35, each region size is 16 × 16), and takes p block images for each region (e.g., the first region AREA11), wherein the p block images of each region are captured under the same color value of the variable color light. In the above example, when all three maximum color values R _ MAX, G _ MAX, and B _ MAX are 255, all three minimum color values R _ MIN, G _ MIN, and B _ MIN are 0, and all three color adjustment values Δ R, Δ G, and Δ B are 255 (8 color values), 1600(8X200) block images of the first AREA11 are captured, and the 1600 block images correspond to different color values (255 ), (0,255,255), (255,0,255, 0,0), (0,0,255), (0,255,0, 0). By analogy, 1600 block images of a second AREA12 are captured for the second AREA12 until all the AREAs (including 3x5 AREAs 11-AREA 35) are completely captured for 1600 block images. That is, a total of mxnxp block images are obtained at each color value, and in the above example, a total of 5 × 3 × 200 block images are captured at each color value.
It should be noted that m, n, and p are only exemplary and not limiting. The setting values can be designed into different values according to actual requirements, and the scope of the invention is also covered by the invention.
It should be noted that, in the above embodiment, each of the mxn regions uses the same three color RANGEs R _ RANGE, G _ RANGE, B _ RANGE and the same three color adjustment values Δ R, Δ G, Δ B to capture the block image, which is only an example and is not a limitation of the present invention. In other embodiments, the different regions may use different three color RANGEs R _ RANGE, G _ RANGE, B _ RANGE (including three maximum color values R _ MAX, G _ MAX, B _ MAX and three minimum color values R _ MIN, G _ MIN, B _ MIN) or different three color adjustment values Δ R, Δ G, Δ B to capture the block image. In this case, different three color RANGEs R _ RANGE, G _ RANGE, B _ RANGE (including three maximum color values R _ MAX, G _ MAX, B _ MAX and three minimum color values R _ MIN, G _ MIN, B _ MIN) and/or different three color adjustment values Δ R, Δ G, Δ B are input for the respective regions. For example, the three maximum color values R _ MAX, G _ MAX, and B _ MAX of the AREA11 may be set to 255, and 255, the three minimum color values R _ MIN, G _ MIN, and B _ MIN may be set to 0, and 0, the three color adjustment values Δ R, Δ G, and Δ B may be set to 255, and 255, the three maximum color values R _ MAX, G _ MAX, and B _ MAX of the AREA12 may be set to 255,0, and 210, the three minimum color values R _ MIN, G _ MIN, and B _ MIN may be set to 51, 0, and 180, the three color adjustment values Δ R, Δ G, and Δ B may be set to 255,0, and 15, the three maximum color values R _ MAX, G _ MAX, and B _ MAX of the AREA13 may be set to 150, 180, and 0, the three minimum color adjustment values R _ MIN, G, and Δ B may be set to 75, 150, and 0, the three color adjustment values Δ R, Δ G, and Δ B may be set to 15, respectively, 5. 0, and so on, this can accomplish three different color RANGEs R _ RANGE, G _ RANGE, and B _ RANGE (three different maximum color values R _ MAX, G _ MAX, and B _ MAX, and three different minimum color values R _ MIN, G _ MIN, and B _ MIN) for different regions.
In one possible embodiment, the three color RANGEs R _ RANGE, G _ RANGE, and B _ RANGE of the color-changeable light may include three maximum color values R _ MAX, G _ MAX, and B _ MAX and three corresponding decrementing times N _ R, N _ G, N _ B. For example, the user may set the three maximum color values of the three color RANGEs R _ RANGE, G _ RANGE, and B _ RANGE of the variable color light to 255, and 255 respectively, set the three decrement times N _ R, N _ G, N _ B to 3, 0, and 2, and set the three color adjustment values Δ R, Δ G, and Δ B to 15, 0, and 51 respectively, so that the control unit 140 first adjusts the variable color light of the variable color light source 120 to (255, and 255), and at this time, the imaging unit 150 captures a plurality of images of the object OB1 corresponding to the variable color light 255; then, after every predetermined time interval (for example, every 30 seconds or after the imaging unit 150 has captured all the images of the object OB 1), the control unit 140 first decrements the color-changeable light source 120 by one of the color adjustment values Δ R, Δ G, Δ B each time, and sequentially decrements and adjusts the color-changeable light source to (240,255,255), (225,255,255), (210,255,255), (255,255,204), (255,255,153), (240,255,255), (240,255,204), (240,255,153), (225,255,255), (225,255,204), (225,255,153), (210,255,255), (210,255,204), (210,255,153) until the color value of the color-changeable light source 120 is adjusted to (210,255,153). In this process, the color value R is set to 255, 240, 225, 210 in order (3 decrements in total), the color value G is set to 255 (0 decrements in total), and the color value B is set to 255,204, 153 in order (2 decrements in total).
In another possible embodiment, the three color ranges of the variable color light include the three minimum color values and the corresponding three increasing times, which also falls within the scope of the present invention.
In the determination process, firstly, the determining unit 370 defaults a first image as a main image to be finally output, then calculates the sharpness of p block images for each region calculating unit 360, and if the sharpness of a specific block image is greater than the highest sharpness of the region on the main image, updates the highest sharpness value, and simultaneously replaces the default block image of the region on the main image with the specific block image. For example, if the color value is (255 ), the determining unit 370 first defaults the first block images of all the AREAs AREA 11-AREA 35 to be the final output main image, and the first block images of all the AREAs correspond to the color value (255 ). Then, for the first AREA11, the calculating unit 360 calculates the sharpness of 200 block images, and if the sharpness of a specific block image is greater than the highest sharpness of the first AREA11 on the main image, the highest sharpness value is updated, and the determining unit 370 replaces the default block image (i.e., the first block image) of the first AREA11 on the main image with the specific block image. When the sharpness of all the 200 block images of the first AREA11 is compared, the determining unit 370 determines a sharpest block image. By analogy, for all the AREAs AREA11 to AREA35, the calculating unit 360 calculates the sharpness of 200 block images, and the final determining unit 370 determines the clearest block images of each of the AREAs AREA11 to AREA35, and combines the clearest block images into the final output main image. Thus, there is a clearest main image for each color value, and in the first example, the invention generates 8 clearest main images (8 color values). Each main image is spliced by the clearest AREAs AREA 11-AREA 35.
In one possible embodiment, the invention calculates the square of the difference between two adjacent pixels to calculate the sharpness of the image, that is, the calculating unit 360 may calculate the sharpness of the plurality of images using a first function, where the first function is:
D(f)＝∑y∑x|f(x+2，y)-f(x，y)|2 (1)；
in the formula: f (x, y) represents the gray value of the pixel point (x, y) corresponding to the image f, and D (f) is the image definition calculation result.
In another possible embodiment, the invention calculates the sharpness of the image by calculating the energy gradient, that is, the calculating unit 360 may calculate the sharpness of the plurality of images by using a second function, where the second function is:
D(f)＝∑y∑x(|f(x+1，y)-f(x，y)|2+|f(x，y+1)-f(x，y)|2) (2)；
in the formula: f (x, y) represents the gray value of the pixel point (x, y) corresponding to the image f, and D (f) is the image definition calculation result.
In another possible embodiment, the invention calculates the sharpness of the image by multiplying two gray differences in each pixel region and then accumulating the multiplied differences one by one, that is, the calculating unit 360 may calculate the sharpness of the plurality of images by using a third function, where the third function is:
D(f)＝∑y∑x(|f(x，y)-f(x，y-1)|+|f(x，y)-f(x+1，y)|) (3)；
in the formula: f (x, y) represents the gray value of the pixel point (x, y) corresponding to the image f, and D (f) is the image definition calculation result.
It should be understood by those skilled in the art that the above-mentioned first function, second function and third function can be used individually or simultaneously, and are within the scope of the present invention. In a possible embodiment of the present invention, the calculating unit calculates the sharpness of the block image corresponding to a first group of regions using the first function, calculates the sharpness of the block image corresponding to a second group of regions using the second function, and calculates the sharpness of the block image corresponding to a third group of regions using the third function. For example, the first function may be used to calculate the plurality of block images corresponding to the AREAs AREA 11-AREA 15, the second function may be used to calculate the plurality of block images corresponding to the AREAs AREA 21-AREA 25, and the third function may be used to calculate the plurality of block images corresponding to the AREAs AREA 31-AREA 35, which are only exemplary and not limitations of the present invention.
Please refer to the following Table _1, where Table _1 represents the highest definition obtained by calculating the plurality of block images in different areas by using the first function, the second function, and the third function, respectively. As can be seen from Table _1, the AREAs AREA11 to AREA15 have the highest sharpness (880) obtained by calculating the plurality of block images by using the first function, the AREAs AREA21 to AREA25 have the highest sharpness (822) obtained by calculating the plurality of block images by using the second function, and the AREAs AREA31 to AREA35 have the highest sharpness (937) obtained by calculating the plurality of block images by using the third function.
Table_1
Referring to fig. 5, fig. 5 is a flowchart illustrating a method for controlling a color-dependent microscope imaging system according to an embodiment of the invention. The color-dependent microscope imaging system comprises a variable color light source for providing variable color light to an object and an imaging unit for photographing the object to generate a plurality of images per second. The method comprises the following steps:
s510: providing a user input interface for inputting three color ranges and three color adjustment values of the variable color light to a user;
s520: adjusting the variable color light of the variable color light source according to the three color ranges and the three color adjustment values input by the user; and
s530: when the color-changeable light of the color-changeable light source is adjusted according to the three color ranges and the three color adjustment values, the imaging unit is controlled to capture a plurality of images of the color-changeable light under different color values.
By means of the technical scheme, the invention has the following beneficial effects: the clearest images of different variable color lights shot in the microscope visual field are displayed on a screen after being spliced, so that the overall view of a researched object in the microscopic visual field can be conveniently checked, and an interested area can be conveniently researched.
The principle and the implementation mode of the invention are explained by applying specific embodiments in the invention, and the description of the embodiments is only used for helping to understand the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, there may be variations in the specific embodiments and the application scope, and in summary, the content of the present specification should not be construed as a limitation to the present invention.
Claims (10)
1. A color correlated microscopy imaging system comprising:
the object carrying platform is used for placing a target object to be observed;
the color-variable light source is positioned above the object platform and used for providing color-variable light for the object;
the user input interface is used for providing a user to input three color ranges and three color adjusting values of the variable color light;
a control unit, coupled to the variable color light source and the user input interface, for adjusting the variable color light of the variable color light source according to the three color ranges and the three color adjustment values input by the user; and
an imaging unit for photographing the target object to generate a plurality of images per second;
when the control unit adjusts the color-changeable light of the color-changeable light source according to the three color ranges and the three color adjustment values, the imaging unit captures a plurality of images of the color-changeable light under different color values.
2. The color-dependent microscopy imaging system of claim 1, wherein the three color ranges comprise three maximum color values and three minimum color values, the control unit first adjusts the variable color light of the variable color light source to the three maximum color values and then decrements the variable color light of the variable color light source according to one of the three color adjustment values after every predetermined time interval until the variable color light of the variable color light source is adjusted to the three minimum color values.
3. The color correlated microscopy imaging system of claim 1, further comprising:
a calculating unit, coupled to the imaging unit, for calculating the sharpness of the plurality of images; and
a determining unit, coupled to the calculating unit, for determining a clearest image from the plurality of images.
4. The color correlated microscopy imaging system of claim 3, wherein the imaging unit divides the object into mxn regions, and takes p block images for each region, wherein the p block images for each region are captured at the same color value of the color variable light.
5. The color-dependent microscopy imaging system of claim 4, wherein the determining unit defaults a first image as a final output main image, and then for each region, the calculating unit calculates the sharpness of p block images, and if the sharpness of a particular block image is greater than the highest sharpness of the region on the main image, updates the highest sharpness value, while the determining unit replaces the default block image of the region on the main image with the particular block image.
6. The color correlated microscopy imaging system of claim 3, wherein the calculation unit calculates the sharpness of the plurality of images using a first function, the first function being formulated as:
D(f)＝∑y∑x|f(x+2，y)-f(x，y)|2 (1)；
in the formula: f (x, y) represents the gray value of the pixel point (x, y) corresponding to the image f, and D (f) is the image definition calculation result.
7. The color correlated microscopy imaging system of claim 3, wherein said calculation unit calculates the sharpness of said plurality of images using a second function, said second function being formulated as:
D(f)＝∑y∑x(|f(x+1，y)-f(x，y)|2+|f(x，y+1)-f(x，y)|2) (2)；
in the formula: f (x, y) represents the gray value of the pixel point (x, y) corresponding to the image f, and D (f) is the image definition calculation result.
8. The color correlated microscopy imaging system of claim 3, wherein the calculating unit calculates the sharpness of the plurality of images using a third function, the third function being formulated as:
D(f)＝∑y∑x(|f(x，y)-f(x，y-1)|+|f(x，y)-f(x+1，y)|) (3)；
in the formula: f (x, y) represents the gray value of the pixel point (x, y) corresponding to the image f, and D (f) is the image definition calculation result.
9. The color correlated microscopy imaging system of any one of claims 6 to 8, wherein the calculation unit uses the first function to calculate the sharpness of block images corresponding to a first set of regions, uses the second function to calculate the sharpness of block images corresponding to a second set of regions, and uses the third function to calculate the sharpness of block images corresponding to a third set of regions.
10. A method of controlling a color-dependent microscopy imaging system, the color-dependent microscopy imaging system including a variable color light source for providing a variable color light to an object and an imaging unit for capturing the object to produce a plurality of images per second, the method comprising the steps of:
providing a user input interface for inputting three color ranges and three color adjustment values of the variable color light to a user;
adjusting the variable color light of the variable color light source according to the three color ranges and the three color adjustment values input by the user; and
when the color-changeable light of the color-changeable light source is adjusted according to the three color ranges and the three color adjustment values, the imaging unit is controlled to capture a plurality of images of the color-changeable light under different color values.
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