CN113355243B - Processing method for microwave-assisted extraction of microalgae active components - Google Patents

Processing method for microwave-assisted extraction of microalgae active components Download PDF

Info

Publication number
CN113355243B
CN113355243B CN202110627098.3A CN202110627098A CN113355243B CN 113355243 B CN113355243 B CN 113355243B CN 202110627098 A CN202110627098 A CN 202110627098A CN 113355243 B CN113355243 B CN 113355243B
Authority
CN
China
Prior art keywords
microalgae
feed liquid
microwave
extraction
raw material
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202110627098.3A
Other languages
Chinese (zh)
Other versions
CN113355243A (en
Inventor
苗钧魁
冷凯良
刘小芳
于源
张雅婷
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
Original Assignee
Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences filed Critical Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
Priority to CN202110627098.3A priority Critical patent/CN113355243B/en
Publication of CN113355243A publication Critical patent/CN113355243A/en
Application granted granted Critical
Publication of CN113355243B publication Critical patent/CN113355243B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids

Abstract

The invention relates to a processing method for microwave-assisted extraction of microalgae active components, belonging to the field of biotechnology, and the method specifically comprises the following steps: the microalgae raw material is subjected to swelling and wall breaking treatment, microwave-assisted extraction and material separation, the feed liquid is used as a microalgae aqueous extract raw material, and the algae residue 1 is used as a subsequent protein peptide extraction raw material; removing impurities from the extracting solution, refining to prepare a microalgae aqueous extract, and performing composite enzymolysis on algae residues to prepare the protein peptide. The method replaces the traditional heating extraction by the degradation effect of microwaves on the cell walls of the microalgae raw materials under the catalysis of transition metals, realizes the full release of water-soluble active components in the raw materials, improves the extraction rate of products by more than 10 percent, greatly reduces the extraction time and improves the extraction efficiency.

Description

Processing method for microwave-assisted extraction of microalgae active components
Technical Field
The invention relates to a method for extracting and processing water-soluble active components (growth factors, phycocyanin, water-soluble polysaccharide and the like) and protein peptides in microalgae (including chlorella, spirulina and the like) by microwave-assisted extraction, belonging to the technical field of biology.
Background
The microalgae industry starts from the 50-60 years of the last century and develops for more than 60 years, the microalgae industry represented by spirulina and chlorella is continuously developed and developed, the global spirulina yield in China is the first, about 9000 tons of spirulina is produced every year, about 3000 tons of chlorella products in China are produced, and the global microalgae culture yield in China is about two thirds. The microalgae products are rich in various nutrient elements such as protein, polysaccharide, vitamins and the like, are widely applied to functional foods and health-care foods, and have higher and higher acceptance degree along with the continuous improvement of health requirements of people, wherein the spirulina is the best food for people in the 21 st century recommended by grain and agriculture organization and world health organization of the United nations. However, the main product forms in the microalgae industry are still the common product forms such as the algae tablet, although the application of the extraction and comprehensive utilization technology of the active components in the microalgae is available, such as: the extraction and preparation of the growth factor (CGF) in Chlorella, and the extraction and preparation of phycocyanin and protein peptide in spirulina are still not achieved, but the full comprehensive utilization of microalgae resources is still not achieved overall, and the development and utilization level of active components in the microalgae resources is still to be improved.
At present, aiming at the existing technology of comprehensively utilizing active components in microalgae, a method for extracting various active substances from spirulina and application thereof provide a method for extracting various active substances from spirulina, but the operation steps are complicated and are not suitable for industrial application; a spirulina bioactive substance grading separation method provides a spirulina active substance grading separation method, which firstly extracts phycocyanin peptide, then obtains glycolipid through supercritical carbon dioxide extraction, and obtains spirulina protein and polysaccharide crude products after drying residual algae residue, thereby realizing the grading utilization of spirulina raw materials; a chlorella growth factor (chlorella essence, CGF) extraction method provides an enzymolysis auxiliary extraction method for chlorella raw materials, and a method for breaking the wall and extracting the chlorella raw materials at low temperature by using pectinase; a Chlorella Growth Factor (CGF) extraction process provides an extraction method combining ultrasound with wall breaking enzyme, and filtration and refining of chlorella are realized through membrane treatment to obtain CGF with low impurity content; the processing and utilizing methods are all traditional extraction methods, and the product yield and the processing efficiency are still improved.
Disclosure of Invention
The invention aims to provide a processing method for improving the comprehensive utilization effect of microalgae resources by microwave degradation. The method replaces the traditional heating extraction by the degradation effect of microwaves on the cell walls of the microalgae raw materials under the catalysis of transition metals, realizes the full release of water-soluble active components in the raw materials, improves the extraction rate of products by more than 10%, greatly reduces the extraction time, improves the extraction efficiency, and provides a simple and efficient processing method for the extraction of the active components in the microalgae raw materials.
In order to solve the technical problems, the invention adopts the technical scheme that:
a processing method for microwave-assisted extraction of microalgae active components specifically comprises the following steps:
(1) swelling and homogenizing wall breaking of raw materials:
firstly, adding 8-10 times of water into microalgae raw material powder, soaking for 2-4h, absorbing water, and swelling to facilitate subsequent extraction reaction, and homogenizing and crushing the swollen material at high pressure of 10-20MPa for 2-3 times;
(2) microwave-assisted extraction and material separation:
transferring the treated material obtained in the step (1) to a storage tank with stirring, and adding a transition metal catalyst FeSO into the material under the stirring state 4 、FeCl 3 Or CuSO 4 Adding 1 of the above materials in an amount of 0.03-0.05% of the water, stirring for 15-20min to dissolve and disperse completely; then, transferring the materials into a microwave reactor, wherein the microwave power is 400-; filtering the material subjected to microwave treatment to realize solid-liquid separation, wherein the feed liquid is used as a microalgae water extract raw material, and the algae residue 1 is used as a subsequent protein peptide extraction raw material;
(3) removing impurities from the extracting solution, refining and preparing a microalgae aqueous extract:
the feed liquid obtained by separation in the step (2) needs to be subjected to catalyst removal and macromolecular impurity removal, so that a microalgae aqueous extract product is obtained; transferring the feed liquid to a storage tank with stirring, and adding Ca (OH) into the feed liquid under stirring 2 Adjusting the pH value to 9.0, standing for 20min, and filtering with diatomite to remove impurities; after filtering, adding HCl into the feed liquid to adjust the pH value to 7.0-8.0, and removing macromolecular impurities in the feed liquid through a ceramic membrane or a hollow fiber membrane; sterilizing the feed liquid after removing impurities to obtain a microalgae aqueous extract;
(4) preparing protein peptide from algae residue by composite enzymolysis:
transferring the algae residue 1 obtained by separation in the step (2) to an enzymolysis tank, adding about the same amount of purified water, adding 1-2 of alkaline protease, neutral protease and papain, and carrying out enzymolysis for 3h at 60 ℃; after enzymolysis, the material is centrifugally separated to obtain enzymolysis liquid and algae residue 2, and the algae residue 2 is dried to be used as a feed raw material; decolorizing the enzymolysis solution with active carbon, microfiltering to remove visible impurities, concentrating the treated feed liquid by nanofiltration to solid content of 10-12%, adding purified water, desalting until the conductivity of the permeate reaches 0.2mS cm -1 Then, the desalting is stopped and the concentration is continuedThe solid content of the concentrated liquid is 10 to 12 percent; carrying out spray drying on the concentrated feed liquid to obtain microalgae protein peptide;
compared with the prior art, the invention has the beneficial effects that:
1. compared with the traditional heating extraction, the microwave-assisted extraction has the following advantages: firstly, the full release of water-soluble active components is realized through the microwave degradation effect on the cell wall tissues of the microalgae raw materials under the catalysis of transition metals, and the extraction rate of products is improved by more than 10%; secondly, the original macromolecular substances are degraded into micromolecular substances with better activity, so that the activity of the product is improved, and the development of subsequent products is facilitated; thirdly, the extraction efficiency is greatly improved by microwave-assisted extraction, and the original extraction time of more than 1h is shortened to 3-5 min;
2. the novel process technology realizes the comprehensive utilization of microalgae raw material resources by extracting the water extract and the protein peptide step by step, has simple steps and strong operability, is convenient to realize industrial transformation, and provides a feasible technical scheme for the high-valued comprehensive utilization of microalgae resources.
Detailed Description
The technical solution of the present invention will be described in detail with reference to the following examples
Example 1
The processing method for microwave-assisted extraction of the active components of the microalgae specifically comprises the following steps:
(1) swelling and homogenizing wall breaking of raw materials:
the microalgae raw materials need swelling and wall breaking treatment, firstly, 8-10 times of water is added into microalgae raw material algae powder for soaking, water absorption and swelling for 2-4 hours, in order to be more beneficial to subsequent extraction reaction, the swelled materials are subjected to high-pressure homogenization and crushing, the homogenization pressure is 10-20MPa, and the homogenization times are 2-3 times;
(2) microwave-assisted extraction and material separation:
transferring the treated material obtained in the step (1) to a storage tank with stirring, and adding a transition metal catalyst FeSO into the material under the stirring state 4 Or CuSO 4 Adding 1 of the above materials in an amount of 0.03-0.05% of the water, stirring for 15-20min to dissolve and disperse completely; then, willTransferring the material into a microwave reactor, wherein the microwave power is 400-800w, and the microwave time is 3-5 min; filtering the material subjected to microwave treatment to realize solid-liquid separation, wherein the feed liquid is used as a chlorella water extract raw material, and the chlorella residue 1 is used as a subsequent protein peptide extraction raw material;
(3) removing impurities from the extracting solution, refining and preparing a chlorella water extract:
the feed liquid obtained by separation in the step (2) needs to be subjected to catalyst removal and macromolecular impurity removal, so that a microalgae aqueous extract product is obtained; transferring the feed liquid to a storage tank with stirring, and adding Ca (OH) into the feed liquid under stirring 2 Adjusting the pH value to 9.0, standing for 20min, and filtering with diatomite to remove impurities; after filtering, adding HCl into the feed liquid to adjust the pH value to 7.0-8.0, and removing macromolecular impurities in the feed liquid through a ceramic membrane or a hollow fiber membrane; removing impurities to obtain extractive solution, i.e. Chlorella water extract;
(4) preparing protein peptide from algae residue by composite enzymolysis:
transferring the algae residue 1 obtained by separation in the step (2) to an enzymolysis tank, adding purified water with the same amount, adding 1-2 of alkaline protease, neutral protease and papain, and carrying out enzymolysis for 3h at 60 ℃; after enzymolysis, the material is centrifugally separated to obtain enzymolysis liquid and algae residue 2, and the algae residue 2 is dried to be used as a feed raw material; decolorizing the enzymolysis solution with active carbon, microfiltering to remove visible impurities, nanofiltering and concentrating the treated material liquid to solid content of 10-12%, adding purified water, desalting until the permeability of the permeate reaches 0.2mS cm -1 Stopping desalting, and continuously concentrating the solid content of the feed liquid to 10-12%; and (4) carrying out spray drying on the concentrated feed liquid to obtain the microalgae protein peptide.
Example 1: the microalgae raw material is chlorella powder, and the consumption of the raw material is 10 kg; adding purified water with the weight 8 times that of the chlorella powder raw material in the step (1), soaking for 2 hours, homogenizing under the pressure of 20MPa, and homogenizing for 2 times; adding a transition metal catalyst CuSO according to the step (2) 4 The adding amount is 0.05 percent of the adding water amount, the microwave power is 800w, the microwave time is 3min, and the solid-liquid separation of the materials is realized by plate-frame filtration; adjusting the pH of the feed liquid to 8.0 after the filtration in the step (3), and removing macromolecular impurities in the feed liquid by selecting a ceramic membrane; adding a base for step (4)Sexual protease, the addition amount is 0.6% of the weight of chlorella raw material respectively, nanofiltration is concentrated to the solid content of 12%;
(1) swelling and homogenizing wall breaking of raw materials:
adding 80kg purified water into a stainless steel enzymolysis tank, adding 10kg chlorella powder raw material while stirring, soaking for 4h under stirring at normal temperature to swell, and breaking cell wall for 2 times by passing through a high-pressure homogenizer at homogenizing pressure of 20 MPa;
(2) microwave-assisted extraction and material separation:
transferring the treated material in the step (1) to a storage tank with stirring, and adding CuSO into the material under the stirring state 4 The adding amount is 50g, and the mixture is stirred for 20min after being added, so that the mixture is fully dissolved and uniformly dispersed; then, transferring the materials into a microwave reactor, wherein the microwave power is 800w, and the microwave time is 3 min; filtering the material subjected to microwave treatment to realize solid-liquid separation to obtain 65.3kg of feed liquid serving as a chlorella water extract raw material, and using the chlorella residue as a subsequent protein peptide extraction raw material;
(3) removing impurities from the extracting solution, refining and preparing a chlorella water extract:
transferring the feed liquid in the step (2) to a storage tank with a stirrer, and adding Ca (OH) into the feed liquid under the stirring state 2 Adjusting pH to 9.0, standing for 20min, and filtering with diatomite filter to remove impurities; after filtering, adding HCl into the feed liquid to adjust the pH value to 8.0, and removing macromolecular impurities in the feed liquid through a ceramic membrane; the feed liquid after impurity removal is subjected to heating sterilization treatment at 80 ℃ to obtain a chlorella water extract, the total amount of a final product is 72.5kg, the solid content is 4.05 percent, the extraction rate is 29.36 percent, and the molecular weight of the product is less than 5000 percent and 95 percent;
(4) preparing protein peptide from algae residue through composite enzymolysis:
transferring the algae residue 1 obtained by separation in the step (2) to an enzymolysis tank, adding 40kg of purified water, adding 0.06kg of alkaline protease, and carrying out enzymolysis for 3h at 60 ℃; centrifuging and separating the material after enzymolysis by using a horizontal screw to obtain enzymolysis liquid and algae residues 2, and drying the algae residues 2 to be used as a feed raw material; decolorizing the enzymolysis liquid with active carbon, and treating with diatomite filter to remove visible impurities; the microfiltration feed liquid is nanofiltered and concentrated until the solid content is 12 percent, and then pure is addedDesalting with purified water until the electric conductivity of the permeate reaches 0.2mS cm -1 Stopping desalting, and continuously concentrating the feed liquid to 16.5kg with solid content of 11.8%; concentrating, spray drying to obtain Chlorella protein peptide 1.65 kg.
Example 2: the microalgae raw material is spirulina powder, and the consumption of the raw material is 20 kg; adding purified water with the weight 10 times that of the chlorella powder raw material in the step (1), soaking for 4 hours, homogenizing under the homogenizing pressure of 10MPa, and homogenizing for 3 times; adding a transition metal catalyst FeSO according to the step (2) 4 The adding amount is 0.03 percent of the adding water amount, the microwave power is 400w, the microwave time is 2min, and the solid-liquid separation of the materials is realized by plate-frame filtration; aiming at the filtering in the step (3), adjusting the pH of the feed liquid to 7.0, and selecting a hollow fiber membrane to remove macromolecular impurities in the feed liquid; adding neutral protease and papain in the step (4), wherein the addition amounts of the neutral protease and the papain are respectively 0.6 percent and 0.3 percent of the weight of the chlorella raw material, and performing nanofiltration concentration until the solid content is 10 percent;
(1) swelling and homogenizing wall breaking of raw materials:
adding 200kg purified water into a stainless steel enzymolysis tank, adding 20kg chlorella powder raw material while stirring, soaking for 4h under stirring at normal temperature to swell, and breaking cell wall for 3 times by passing through a high-pressure homogenizer at homogenizing pressure of 10 MPa;
(2) microwave-assisted extraction and material separation:
transferring the treated material obtained in the step (1) to a storage tank with stirring, and adding FeSO into the material under the stirring state 4 The addition amount is 60g, and the mixture is stirred for 20min after the addition to be fully dissolved and uniformly dispersed; then, transferring the materials into a microwave reactor, wherein the microwave power is 400w, and the microwave time is 2 min; filtering the material subjected to microwave treatment to realize solid-liquid separation to obtain 165.5kg of feed liquid serving as a raw material of a spirulina water extract, and taking the algae residue 1 as a raw material for extracting subsequent protein peptides;
(3) removing impurities from the extracting solution, refining and preparing the spirulina water extract:
transferring the feed liquid of the step (2) to a storage tank with stirring, and adding Ca (OH) into the feed liquid under the stirring state 2 Adjusting pH to 9.0, standing for 20min, and filtering with diatomite filter to remove impurities; after filtration, toAdding HCl into the feed liquid to adjust the pH value to 7.0, and removing macromolecular impurities in the feed liquid through a hollow fiber membrane; after the impurities are removed, the feed liquid is heated and sterilized at 80 ℃ to obtain the spirulina water extract, the total amount of the final product is 162.3kg, the solid content is 5.05 percent, the extraction rate is 40.98 percent, and the molecular weight of the product is less than 5000 percent to 95 percent;
(4) preparing protein peptide from algae residue by composite enzymolysis:
transferring the algae residue 1 obtained by separation in the step (2) into an enzymolysis tank, adding 70kg of purified water, adding 12g of sexual protease and 6g of papain respectively, and carrying out enzymolysis for 3h at 60 ℃; after enzymolysis, centrifugally separating the material by using a horizontal screw to obtain enzymolysis liquid and algae residues 2, and drying the algae residues 2 to be used as feed raw materials; decolorizing the enzymolysis liquid with active carbon, and treating with diatomite filter to remove visible impurities; concentrating the microfiltered feed liquid by nanofiltration until the solid content is 10 percent, adding purified water for desalination until the electric conductivity of the permeate is 0.2 mS.cm -1 Stopping desalting, and continuing to concentrate the feed liquid to 52.8kg and the solid content to 10.2%; the concentrated feed liquid is sprayed and dried to obtain 5.21kg of spirulina protein peptide.
Example 3: the microalgae raw material is chlorella powder, and the consumption of the raw material is 10 kg; adding purified water which is 8 times of the weight of the chlorella powder raw material in the step (1), soaking for 2 hours, homogenizing under 20MPa, and homogenizing for 2 times; no catalyst is added, the microwave power is 800w, the microwave time is 3min, and the solid-liquid separation of the materials is realized by plate-and-frame filtration; adding alkaline protease in the step (4), wherein the addition amount of the alkaline protease is 0.6 percent of the weight of the chlorella raw material respectively, and performing nanofiltration concentration until the solid content is 12 percent;
(1) swelling and homogenizing wall breaking of raw materials:
adding 80kg purified water into a stainless steel enzymolysis tank, adding 10kg chlorella powder raw material while stirring, soaking for 4h under stirring at normal temperature to swell, and breaking cell wall for 2 times by passing through a high-pressure homogenizer at homogenizing pressure of 20 MPa;
(2) microwave-assisted extraction and material separation:
transferring the materials into a microwave reactor, wherein the microwave power is 800w, and the microwave time is 3 min; filtering the material subjected to microwave treatment to realize solid-liquid separation to obtain 60.5kg of feed liquid serving as a chlorella water extract raw material, and taking the chlorella residue 1 as a subsequent protein peptide extraction raw material;
(3) removing impurities from the extracting solution, refining and preparing a chlorella water extract:
transferring the feed liquid obtained in the step (2) to a storage tank with a stirrer, filtering the feed liquid by using a diatomite filter to remove impurities, and removing macromolecular impurities in the feed liquid by using a ceramic membrane; the feed liquid after impurity removal is heated and sterilized at 80 ℃ to obtain the chlorella aqueous extract, the total amount of the final product is 65.5kg, the solid content is 3.65 percent, the extraction rate is 23.91 percent, and the molecular weight of the product is less than 5000 percent and accounts for 60.3 percent;
(4) preparing protein peptide from algae residue by composite enzymolysis:
transferring the algae residue 1 separated in the step (2) into an enzymolysis tank, adding 40kg of purified water, adding 0.06kg of alkaline protease, and carrying out enzymolysis at 60 ℃ for 3 h; after enzymolysis, centrifugally separating the material by using a horizontal screw to obtain enzymolysis liquid and algae residues 2, and drying the algae residues 2 to be used as feed raw materials; decolorizing the enzymolysis liquid with active carbon, and treating with diatomite filter to remove visible impurities; concentrating the microfiltered feed liquid by nanofiltration until the solid content is 12 percent, adding purified water for desalination until the electric conductivity of the permeate is 0.2 mS.cm -1 Stopping desalting, and continuing to concentrate the feed liquid to 18.5kg and the solid content to 11.8%; the concentrated feed liquid is sprayed and dried to obtain 1.87kg of chlorella protein peptide.
Example 4: the microalgae raw material is spirulina powder, and the consumption of the raw material is 20 kg; adding purified water with the weight 10 times that of the chlorella powder raw material in the step (1), soaking for 4 hours, homogenizing under the homogenizing pressure of 10MPa, and homogenizing for 3 times; no catalyst is added, the microwave power is 400w, the microwave time is 2min, and the solid-liquid separation of the materials is realized by plate-and-frame filtration; selecting a hollow fiber membrane to remove macromolecular impurities in the feed liquid; adding neutral protease and papain in the step (4), wherein the addition amounts of the neutral protease and the papain are respectively 0.6 percent and 0.3 percent of the weight of the chlorella raw material, and performing nanofiltration concentration until the solid content is 10 percent;
(1) swelling and homogenizing wall breaking of raw materials:
adding 200kg purified water into a stainless steel enzymolysis tank, adding 20kg Chlorella powder under stirring, soaking for 4 hr under stirring at room temperature for swelling, breaking cell wall by high pressure homogenizer for 3 times, and homogenizing under 10 MPa;
(2) microwave-assisted extraction and material separation:
transferring the material into a microwave reactor with the microwave power of 400w and the microwave time of 2 min; filtering the material subjected to microwave treatment to realize solid-liquid separation to obtain feed liquid 155.5kg serving as a raw material of a spirulina water extract, and using the algae residue as a raw material for extracting subsequent protein peptides;
(3) removing impurities from the extracting solution, refining and preparing the spirulina water extract:
transferring the feed liquid obtained in the step (2) to a storage tank with stirring, filtering by a diatomite filter to remove impurities, and removing macromolecular impurities in the feed liquid through a hollow fiber membrane; the feed liquid after impurity removal is subjected to heating sterilization treatment at 80 ℃ to obtain the spirulina water extract, the total amount of the final product is 172.3kg, the solid content is 4.15 percent, the extraction rate is 35.75 percent, and the molecular weight of the product is less than 5000 percent and 65.1 percent;
(4) preparing protein peptide from algae residue by composite enzymolysis:
transferring the algae residue 1 obtained by separation in the step (2) into an enzymolysis tank, adding 70kg of purified water, adding 12g of sexual protease and 6g of papain respectively, and carrying out enzymolysis for 3h at 60 ℃; after enzymolysis, centrifugally separating the material by using a horizontal screw to obtain enzymolysis liquid and algae residues 2, and drying the algae residues 2 to be used as feed raw materials; decolorizing the enzymolysis liquid with active carbon, and treating with diatomite filter to remove visible impurities; the microfiltration feed liquid is subjected to nanofiltration concentration until the solid content is 10 percent, and then purified water is added for desalination until the electric conductivity of the permeation liquid is 0.2mS cm -1 Stopping desalting, and continuing to concentrate the feed liquid to 58.2kg and the solid content to 11.2%; the concentrated feed liquid is sprayed and dried to obtain 5.63kg of spirulina protein peptide.

Claims (1)

1. A processing method for extracting microalgae active components by microwave assistance is characterized by specifically comprising the following steps:
(1) swelling and homogenizing wall breaking of raw materials:
the microalgae raw material needs swelling and wall breaking treatment, firstly, 8-10 times of water is added into microalgae raw material algae powder for soaking, absorbing water and swelling for 2-4h, and the swelled material is subjected to high-pressure homogenization and crushing, wherein the homogenization pressure is 10-20MPa, and the homogenization times are 2-3 times;
(2) microwave-assisted extraction and material separation:
transferring the treated material obtained in the step (1) to a storage tank with stirring, and adding a transition metal catalyst CuSO into the material under the stirring state 4 The adding amount is 0.03-0.05% of the adding water amount, and stirring for 15-20min after adding to make it fully dissolved and uniformly dispersed; then, transferring the materials into a microwave reactor, wherein the microwave power is 400-; filtering the material subjected to microwave treatment to realize solid-liquid separation, wherein the feed liquid is used as a microalgae water extract raw material, and the algae residue 1 is used as a subsequent protein peptide extraction raw material;
(3) removing impurities from the extracting solution, refining and preparing a microalgae aqueous extract:
the feed liquid obtained by separation in the step (2) needs to be subjected to catalyst removal and macromolecular impurity removal, so that a microalgae aqueous extract product is obtained; transferring the feed liquid to a storage tank with stirring, and adding Ca (OH) into the feed liquid under stirring 2 Adjusting the pH value to 9.0, standing for 20min, and filtering with diatomite to remove impurities; after filtering, adding HCl into the feed liquid to adjust the pH value to 7.0-8.0, and removing macromolecular impurities in the feed liquid through a ceramic membrane or a hollow fiber membrane; sterilizing the feed liquid after removing impurities to obtain a microalgae aqueous extract;
(4) preparing protein peptide from algae residue by composite enzymolysis:
transferring the algae residue 1 obtained by separation in the step (2) to an enzymolysis tank, adding purified water with the same amount, adding 1-2 of alkaline protease, neutral protease or papain, and carrying out enzymolysis for 3h at 60 ℃; centrifuging the material after enzymolysis to obtain enzymolysis liquid and algae residue 2, and drying the algae residue 2 to be used as a feed raw material; decolorizing the enzymolysis solution with active carbon, microfiltering to remove visible impurities, concentrating the treated feed liquid by nanofiltration to solid content of 10-12%, adding purified water, desalting until the conductivity of the permeate reaches 0.2mS cm -1 Stopping desalting, and continuously concentrating the solid content of the feed liquid to 10-12%; and (4) carrying out spray drying on the concentrated feed liquid to obtain the microalgae protein peptide.
CN202110627098.3A 2021-06-04 2021-06-04 Processing method for microwave-assisted extraction of microalgae active components Active CN113355243B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202110627098.3A CN113355243B (en) 2021-06-04 2021-06-04 Processing method for microwave-assisted extraction of microalgae active components

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110627098.3A CN113355243B (en) 2021-06-04 2021-06-04 Processing method for microwave-assisted extraction of microalgae active components

Publications (2)

Publication Number Publication Date
CN113355243A CN113355243A (en) 2021-09-07
CN113355243B true CN113355243B (en) 2022-09-30

Family

ID=77532405

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202110627098.3A Active CN113355243B (en) 2021-06-04 2021-06-04 Processing method for microwave-assisted extraction of microalgae active components

Country Status (1)

Country Link
CN (1) CN113355243B (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2794854A1 (en) * 2010-04-06 2011-10-13 Heliae Development, Llc Methods of and systems for producing biofuels
CN105001971B (en) * 2015-06-26 2019-01-29 新奥科技发展有限公司 A kind of method of two-step method production microalgae bio oil
CN107502550A (en) * 2017-10-23 2017-12-22 宁波大红鹰学院 A kind of method of frustule broken wall treatment
CN111154824A (en) * 2020-01-15 2020-05-15 润科生物工程（福建）有限公司 Industrial production method for obtaining spirulina antioxidant oligopeptide through high-concentration two-step enzymolysis

Also Published As

Publication number Publication date
CN113355243A (en) 2021-09-07

Similar Documents

Publication Publication Date Title
CN109810201B (en) Ultrasonic wave composite acidic water extraction method for cordyceps polysaccharide and cordycepin in cordyceps militaris
CN102488713A (en) Method for preparing sheep placenta extract and sheep placenta hydrolyzed collagen concentrated solution
CN106349405A (en) Method for extracting pectin from shaddock peel through enzymolysis and ultrasonic waves
CN109385414B (en) Purification method of bromelain
CN113355243B (en) Processing method for microwave-assisted extraction of microalgae active components
CN102071180A (en) New process for preparing pineapple bromelain
CN105218598B (en) The method that microwave radiation technology hydrolysis chitin prepares D- aminoglucose hydrochlorides
CN107050217A (en) A kind of preparation method of aloe gel
CN109081787B (en) Process for extracting levodopa from mucuna
CN108101980B (en) Preparation method of high-purity phycocyanin
CN102180991B (en) Comprehensive utilization method of pumpkin
CN113980090B (en) Method for recycling biological source protein by using biological medicine fermentation fungus dreg
CN113355383A (en) Comprehensive utilization and processing method of spirulina
CN112680493A (en) Method for extracting macromolecular collagen peptide by fish scale enzymolysis
CN113372467A (en) Method for preparing chitosan by liquefying chitin
CN114250112A (en) Process for synchronously extracting polyphenol tea oil, tea-oil tree polypeptide and tea-oil tree polysaccharide
CN113425754A (en) Method for combined extraction of flavone and pectin from passion fruit peel
CN112940146A (en) Preparation method of tremella polysaccharide
CN107141365B (en) Method for efficiently purifying phellinus igniarius polysaccharides by repeatedly increasing and decreasing pressure
CN106883312A (en) A kind of extracting method of silkworm excrement pectin
EP3253805B1 (en) Process for obtaining inulin from roots of the cardoon plant.
CN1788822A (en) Low-temperature differential pressure type extractive technique
CN113831421B (en) Combined preparation method of grifola frondosa mycelium polypeptide and beta-glucan
CN115645312B (en) Application of sargassum pallidum polyphenol in preparing whitening cosmetics and preparation method thereof
KR101546861B1 (en) The actinidia extracts by Mechanochemical processing and enzyme treatment method

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant