CN113337419A

CN113337419A - Preparation method of microbial agent for soil acidification area

Info

Publication number: CN113337419A
Application number: CN202110453802.8A
Authority: CN
Inventors: 李雪琛
Original assignee: Individual
Current assignee: Shenyang Huaqingyuan Agricultural Development Co ltd
Priority date: 2021-04-26
Filing date: 2021-04-26
Publication date: 2021-09-03
Anticipated expiration: 2041-04-26
Also published as: CN113337419B

Abstract

The invention discloses a method for preparing a microbial inoculum for a soil acidification area, which belongs to the technical field of microbial inoculum, and creatively adds a process of screening and culturing strains by using soil of a target area, before preparing the microbial inoculum, the target area is sampled, the soil sample is subjected to impurity removal, sterilization and water adding mixing to prepare a screening solution, then the screening solution is screened to screen the strains, and high-quality strains which can survive in the screening solution, namely the strains which can adapt to the soil environment of the target area, are screened, so that when the prepared microbial inoculum is used in the target area, the survival rate of microorganisms in the microbial inoculum can be greatly improved, the effect of the microbial inoculum is effectively improved, the practicability is strong, and a microbial collection ball for collecting the high-quality strains is arranged, can be based on the chemotaxis of the microorganisms, and can be used for quickly collecting the strains, Effectively collect the screened high-quality strains, thereby effectively improving the preparation efficiency.

Description

Preparation method of microbial agent for soil acidification area

Technical Field

The invention relates to the technical field of microbial agents, and particularly relates to a preparation method of a microbial agent for a soil acidification area.

Background

The microbial agent is a viable bacteria preparation prepared by processing fermentation liquor for adsorbing bacteria by using porous substances as adsorbents (such as turf and vermiculite) after industrial production and propagation of target microorganisms (effective bacteria). The microbial inoculum is used for seed dressing or root dipping, has the effects of directly or indirectly improving soil, restoring land capability, preventing soil-borne diseases, maintaining rhizosphere microbial community balance, degrading toxic substances and the like, and can improve the yield of agricultural products, improve the quality of the agricultural products, reduce the using amount of fertilizers, reduce the cost, improve the soil and protect the ecological environment by properly using the agricultural microbial inoculum.

When the microbial agent in the prior art is used in areas with serious soil acidification, because the soil is acidic, microorganisms in the microbial agent easily die in a large scale due to the fact that the microorganisms cannot adapt to the severe soil environment, and the effect and the function of the microbial agent can be greatly reduced. Therefore, a preparation method of the microbial agent for the soil acidification area is provided.

Disclosure of Invention

1. Technical problem to be solved

Aiming at the problems in the prior art, the invention aims to provide a preparation method of a microbial agent for a soil acidification area, which can creatively increase the process of screening and culturing strains by using soil of a target area, wherein before the microbial agent is prepared, sampling is carried out in the target area, impurity removal and sterilization are carried out on the soil sample, water is added for mixing to prepare a screening solution, then the screening solution is screened to screen the strains, and high-quality strains which can survive in the screening solution, namely the strains which can adapt to the soil environment of the target area, so that when the prepared microbial agent is used in the target area, the survival rate of microorganisms in the microbial agent can be greatly improved, the effect of the microbial agent is effectively improved, the practicability is strong, and a microbial collection ball for collecting the high-quality strains is arranged and can be based on the chemotaxis of the microorganisms, can quickly and effectively collect the screened high-quality strains, thereby effectively improving the preparation efficiency.

2. Technical scheme

In order to solve the above problems, the present invention adopts the following technical solutions.

A preparation method of a microbial agent for a soil acidification area comprises the following steps:

s1, taking a proper amount of in-situ soil of the target area as a soil sample, removing impurities from the soil sample, and removing impurities and garbage in the soil sample to obtain an impurity-removed soil sample;

s2, sterilizing the rogue soil sample at high temperature to obtain a sterilized rogue soil sample;

s3, mixing and stirring the sterilized soil sample and water fully, and taking supernate as a screening solution after natural precipitation;

s4, pouring the screening liquid into a screening box, then putting beneficial microorganism strains and a liquid culture medium into the screening box, mixing to form a strain screening culture liquid, and carrying out directional screening on the strains for 2-3 d;

s5, placing the microorganism collecting balls into a screening box, and collecting beneficial microorganism strains surviving in the screening box;

s6, taking out the microbial strains collected by the microbial collecting ball to obtain high-quality strains, and performing propagation on the high-quality strains;

s7, fermenting the high-quality strain propagated in the S6 to obtain a bacterial liquid;

s8, uniformly mixing the bacterial liquid in the S7 with the fermentation material, and then putting the mixture into an oven to be dried to obtain the microbial agent.

Further, the temperature of high-temperature sterilization in the S2 is 90-130 ℃, the sterilization time is 20-40min, and the mass ratio of the sterilization soil removal sample to the water in the S3 is 1: 1-2.

Further, the beneficial microorganism strain in the S4 is one or more of lactic acid bacteria, rhizobia, bacillus and yeast, and the volume ratio of the beneficial microorganism, the culture medium and the screening solution is 1: 20-30: 50-100.

Further, the fermentation material in the S8 is formed by uniformly mixing bean flour and bran according to the volume ratio of 1:4, and the volume ratio of the fermentation material to the bacterial liquid in the S8 is 1: 2-3.

Further, the ball is collected to the microorganism includes hollow spherical shell, the pellicle is installed in the bottom of hollow spherical shell is inlayed, the chemotaxis ball that two symmetries of fixedly connected with set up on the inner wall of hollow spherical shell, the middle part of hollow spherical shell is provided with the elasticity gasbag, equal fixedly connected with gangbar on the outer wall of elasticity gasbag both sides, the through-hole has all been seted up on the outer wall of hollow spherical shell both sides, two the gangbar is kept away from the equal fixedly connected with of one end and through-hole assorted hole stopper of elasticity gasbag, two the hole stopper is pegged graft with two through-holes respectively.

Further, chemotaxis ball intussuseption is filled with glucose solution, a plurality of fluff of adhering to of fixedly connected with on the outer wall of chemotaxis ball, chemotaxis ball adopts the pellicle material to make, and the fenestra on the chemotaxis ball is less than the glucose molecule, and the microorganism is collected the ball and is put into the screening case after, the microbial srain that still survives after the screening fluid screening, also be exactly the high-quality bacterial that can right amount soil acidification environment, can be based on glucose solution to the attractiveness of microorganism, move to chemotaxis ball and be close to in the through-hole gets into hollow spherical shell, and then the completion is collected high-quality bacterial.

Further, elasticity gasbag's inner wall is provided with the design net, the discharge hole has been seted up on the top of hollow spherical shell, the middle part of discharge hole runs through and is provided with the air duct, the air duct runs through the top outer wall of elasticity gasbag, design net in proper order and extends to in the design ball net, equal fixedly connected with bracing piece on the outer wall of air duct both sides, the one end that the air duct was kept away from to the bracing piece and the inner wall fixed connection of hollow spherical shell, the top of air duct is provided with elasticity and beats the gasbag, the top intercommunication that the gasbag was beaten to elasticity has the check valve of breathing in, the bottom intercommunication that the gasbag was beaten to elasticity has the check valve of giving vent to anger, the check valve of giving vent to anger keeps away from elasticity and beats the one end of gasbag and be linked together with the air duct, beat gasbag, gangbar, hole stopper, air duct, elasticity through elasticity gasbag, elasticity, The air suction one-way valve and the air outlet one-way valve are arranged, after the microorganism collecting ball is placed in the screening box, the air bag can be squeezed repeatedly to pump air into the shaping ball net, so that the shaping ball net expands to drive the plug to move outside the hollow ball shell until the plug is separated from the through hole, the through hole is communicated, high-quality bacteria can move close to the chemotactic ball, after collection is finished, the air release valve can be opened to prevent air from the shaping ball net, so that the plug blocks the through hole again, then the microorganism collecting ball is lifted up to control water, so that water in the hollow ball shell flows out through the water control semipermeable membrane, then a proper amount of liquid culture medium is poured into the hollow ball shell, the microorganism collecting ball is inverted, the high-quality bacteria can be poured out through the discharge hole, and the high-quality bacteria can be taken out.

Furthermore, the outer wall of the air duct is fixedly sleeved with the flow guide hopper, so that the elastic inflating bag can be prevented from being dirtied when high-quality strains are poured out, and the outer wall of the hollow spherical shell is fixedly connected with two symmetrically-arranged holding handles, so that the microorganism collecting ball can be conveniently lifted, held and inverted.

Furthermore, all be provided with on the inner wall of screening case both sides with hole stopper assorted locating piece, when extrusion elasticity was beaten the gasbag and was made the hole stopper move to the clean shot shell outward, can make the hole stopper move and stop extrusion elasticity again after offsetting with the locating piece and beat the gasbag, on the one hand, can play a location effect, on the other hand can prevent that the microorganism from collecting the ball and toppling over, after putting into the screening case with microorganism collection ball in S5, the liquid level height of bacterial screening culture solution is not less than the bottom of hole stopper, and liquid level height is low excessively can lead to high-quality bacterial can't get into in the hollow spherical shell through the through-hole.

3. Advantageous effects

Compared with the prior art, the invention has the advantages that:

(1) the method creatively increases the process of utilizing the soil of the target area to perform screening culture on strains, samples are taken in the target area before the microbial inoculum is prepared, impurities are removed from the soil sample, sterilization is performed, water is added for mixing to prepare a screening solution, the strains are screened by the screening solution, high-quality strains which can survive in the screening solution are screened, namely strains which can adapt to the soil environment of the target area are screened, so that the survival rate of microorganisms in the microbial inoculum can be greatly improved when the prepared microbial inoculum is used in the target area, the effect of the microbial inoculum is effectively improved, the practicability is high, a microorganism collecting ball for collecting the high-quality strains is arranged, the microorganism collecting ball can be based on the chemotaxis of the microorganisms, the screened high-quality strains can be quickly and effectively collected, and the preparation efficiency can be effectively improved.

(2) Chemotaxis ball intussuseption is filled with glucose solution, a plurality of fluff of adhering to of fixedly connected with on the outer wall of chemotaxis ball, chemotaxis ball adopts the pellicle material to make, and the membrane hole on the chemotaxis ball is less than the glucose molecule, behind the microorganism collection ball put into the screening box, the microbial srain that still survives after the screening fluid screening, also be exactly the high-quality bacterial that can right amount soil acidification environment, can be based on glucose solution to the attractiveness of microorganism, move near to chemotaxis ball, and in the through-hole gets into hollow spherical shell, and then the completion is collected high-quality bacterial.

(3) After the microorganism collecting balls are placed in the screening box through the arrangement of the elastic air bag, the linkage rod, the hole plug, the air guide pipe, the elastic inflating bag, the air suction one-way valve and the air outlet one-way valve, air can be pumped into the shaping net by repeatedly squeezing the elastic inflating bag to expand the shaping net, thereby driving the hole plug to move towards the outside of the hollow ball shell until the hole plug is separated from the through hole to conduct the through hole, so that the high-quality strains can move close to the chemotactic ball, and after the collection is finished, the air escape valve can be opened to prevent air of the shaping net, so that the hole plug can block the through hole again, then lifting the microorganism collecting ball to control water, making the water in the hollow ball shell flow out through the water control semipermeable membrane, then pouring a proper amount of liquid culture medium into the hollow ball shell, after the microorganism collecting ball is inverted, the high-quality strains can be poured out through the discharge hole, and then the high-quality strains are taken out.

(4) The outer wall of the air duct is fixedly sleeved with the flow guide hopper, so that the elastic inflating bag can be prevented from being dirtied when high-quality strains are poured out, and the outer wall of the hollow spherical shell is fixedly connected with two symmetrically-arranged holding handles, so that the microorganism collecting ball can be conveniently lifted and inverted.

(5) All be provided with on the inner wall of screening case both sides with stopple assorted locating piece, when extrusion elasticity was beaten the gasbag and was made the stopple move outside the clean shot shell, can make the stopple move to and stop extrusion elasticity again and beat the gasbag after offsetting with the locating piece, on the one hand, can play a location effect, on the other hand, can prevent that microorganism from collecting the ball and topple over, after placing microorganism collection ball into the screening case in S5, the liquid level height of bacterial screening culture solution is not less than the bottom of stopple, liquid level height is low can lead to high-quality bacterial can't get into in the clean shot shell through the through-hole.

Drawings

FIG. 1 is a flow chart of the preparation of the present invention;

FIG. 2 is a schematic sectional view of a microorganism collecting ball according to the present invention;

FIG. 3 is a schematic cross-sectional view of the elastic bladder of the present invention;

FIG. 4 is a schematic cross-sectional view of the diversion hopper according to the present invention;

FIG. 5 is a schematic cross-sectional view of a chemotactic sphere of the present invention;

FIG. 6 is a schematic view showing the structure of the microorganism collecting beads of the present invention when they are placed in a screening chamber.

The reference numbers in the figures illustrate:

101. a hollow spherical shell; 102. a water-controlling semipermeable membrane; 103. a chemotactic sphere; 104. an elastic air bag; 105. shaping the net; 106. a linkage rod; 107. a hole plug; 108. a discharge hole; 109. an air duct; 110. a support bar; 111. elastically inflating the air bag; 112. an air suction check valve; 113. an air outlet one-way valve; 114. a gas release valve; 115. a flow guide hopper; 116. holding a handle; 117. a glucose solution; 118. attaching fluff; 002. a screening box; 201. and (5) positioning the blocks.

Detailed Description

The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention; it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by those skilled in the art without any inventive work are within the scope of the present invention.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top/bottom", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "sleeved/connected," "connected," and the like are to be construed broadly, e.g., "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Example 1:

referring to fig. 1 and 6, a method for preparing a microbial agent for a soil acidification area includes the following steps:

s2, sterilizing the rogue soil sample at high temperature for 20-40min at 90-130 deg.C to obtain sterilized rogue soil sample;

s3, fully mixing and stirring the sterilized impurity-removed soil sample and water, wherein the mass ratio of the sterilized impurity-removed soil sample to the water is 1: 1-2, taking supernatant as screening liquid after natural precipitation;

s4, pouring the screening liquid into the screening box 002, then adding beneficial microorganism strains and a liquid culture medium into the screening box 002, mixing to form a strain screening culture solution, and carrying out directional screening on strains, wherein the beneficial microorganism strains are one or more of lactic acid bacteria, rhizobia, bacillus and saccharomycetes, and the volume ratio of the beneficial microorganism, the culture medium and the screening liquid is 1: 20-30: 50-100, and the screening time is 2-3 d;

s5, placing the microorganism collecting balls into a screening box 002, and collecting beneficial microorganism strains surviving in the screening box 002;

s8, uniformly mixing the bacterial liquid in the step S7 with a fermentation material, wherein the fermentation material is formed by uniformly mixing bean flour and bran according to the volume ratio of 1:4, and the volume ratio of the fermentation material to the bacterial liquid is 1: 2-3, and then drying in an oven to obtain the microbial agent.

Referring to fig. 2 and 5, the microorganism collecting ball includes a hollow spherical shell 101, a water-controlling semipermeable membrane 102 is mounted at the bottom end of the hollow spherical shell 101, two symmetrically arranged chemotactic balls 103 are fixedly connected to the inner wall of the hollow spherical shell 101, an elastic air bag 104 is arranged at the middle part of the hollow spherical shell 101, linkage rods 106 are fixedly connected to the outer walls of both sides of the elastic air bag 104, through holes are formed in the outer walls of both sides of the hollow spherical shell 101, hole plugs 107 matched with the through holes are fixedly connected to one ends of the two linkage rods 106 far away from the elastic air bag 104, the two hole plugs 107 are respectively inserted into the two through holes, a glucose solution 117 is filled in the chemotactic balls 103, a plurality of attached villi 118 are fixedly connected to the outer walls of the chemotactic balls 103, the chemotactic balls 103 are made of a semipermeable membrane material, membrane pores on the chemotactic balls 103 are smaller than glucose molecules, after the microorganism collecting balls are placed in a screening box 002, microorganism strains survive after being screened by a screening liquid, that is, the high-quality strains in the appropriate soil acidification environment move toward the chemotactic ball 103 and enter the hollow spherical shell 101 through the through hole based on the attractiveness of the glucose solution 117 to the microorganisms, thereby completing the collection of the high-quality strains.

Referring to fig. 2-4, a shaped ball net 105 is disposed on an inner wall of the elastic air bag 104, an exhaust hole 108 is disposed at a top end of the hollow ball shell 101, an air duct 109 penetrates through a middle portion of the exhaust hole 108, the air duct 109 sequentially penetrates through the elastic air bag 104 and an outer wall of a top end of the shaped ball net 105 and extends into the shaped ball net 105, support rods 110 are fixedly connected to outer walls of two sides of the air duct 109, one end of each support rod 110, which is far away from the air duct 109, is fixedly connected to an inner wall of the hollow ball shell 101, an elastic inflating bag 111 is disposed above the air duct 109, a suction check valve 112 is communicated with a top end of the elastic inflating bag 111, a discharge check valve 113 is communicated with a bottom end of the elastic inflating bag 111, one end of the discharge check valve 113, which is far away from the elastic inflating bag 111, is communicated with the air duct 109, a discharge valve 114 is disposed on an outer wall of one side of the air duct 109, and the discharge check valve 114 is disposed on an outer wall of the side of the air duct 109 and extends through the elastic air bag 104 and the linkage rod 106, The setting of the plug 107, the air duct 109, the elastic inflating bag 111, the air suction check valve 112 and the air outlet check valve 113, after the microorganism collecting ball is placed in the screening box 002, the elastic inflating bag 111 can be repeatedly squeezed to inject air into the shaped ball net 105 to expand the shaped ball net 105, so as to drive the plug 107 to move towards the outside of the hollow ball shell 101 until the plug 107 is separated from the through hole, so that the through hole is communicated, so that the high-quality strains can move towards the chemotactic ball 103, and after the collection is finished, the air release valve 114 can be opened to prevent air from the shaped ball net 105, so that the plug 107 blocks the through hole again, then the microorganism collecting ball is lifted up to control water, so that the water in the hollow ball shell 101 flows out through the water control semipermeable membrane 102, then a proper amount of liquid culture medium is poured into the hollow ball shell 101, the microorganism collecting ball is inverted, the high-quality strains can be poured out through the discharge hole 108, so as to take out the high-quality strains, the outer wall of the air duct 109 is fixedly sleeved with a flow guide hopper 115 which can prevent the elastic inflating bag 111 from being dirtied when high-quality strains are poured out, and the outer wall of the hollow spherical shell 101 is fixedly connected with two symmetrically arranged holding handles 116 which can facilitate the carrying and the inversion of the microorganism collecting ball.

Referring to fig. 6, the inner walls of the two sides of the screening box 002 are provided with positioning blocks 201 matched with the hole plugs 107, when the elastic inflating bag 111 is squeezed to move the hole plugs 107 out of the hollow spherical shell 101, the hole plugs 107 can be moved to abut against the positioning blocks 201 and then the elastic inflating bag 111 is stopped being squeezed, on one hand, a positioning effect can be achieved, on the other hand, the microorganism collecting balls can be prevented from toppling over, after the microorganism collecting balls are placed into the screening box 002 in S5, the liquid level of the strain screening culture solution is not lower than the bottom ends of the hole plugs 107, and high-quality strains cannot enter the hollow spherical shell 101 through the through holes due to too low liquid level.

The invention creatively increases the process of utilizing the soil of a target area to perform screening culture on strains, samples are taken in the target area before preparing the microbial inoculum, impurities are removed from the soil sample, sterilization and water mixing are performed to obtain a screening solution, the strains are screened by the screening solution to screen out high-quality strains which can survive in the screening solution, namely strains which can adapt to the soil environment of the target area, so that the survival rate of microorganisms in the microbial inoculum can be greatly improved when the prepared microbial inoculum is used in the target area, the effect of the microbial inoculum is effectively improved, the practicability is strong, and the microbial collection ball for collecting the high-quality strains is arranged, can quickly and effectively collect the screened high-quality strains based on the chemotaxis of the microorganisms, thereby effectively improving the preparation efficiency.

The foregoing is only a preferred embodiment of the present invention; the scope of the invention is not limited thereto. Any person skilled in the art should be able to cover the technical scope of the present invention by equivalent or modified solutions and modifications within the technical scope of the present invention.

Claims

1. A preparation method of a microbial agent for a soil acidification area is characterized by comprising the following steps: the method comprises the following steps:

s4, pouring the screening liquid into a screening box (002), then adding beneficial microbial strains and a liquid culture medium into the screening box (002), mixing to form a strain screening culture liquid, and carrying out directional screening on the strains for 2-3 d;

s5, putting the microorganism collecting balls into a screening box (002), and collecting beneficial microorganism strains living in the screening box (002);

2. The method for preparing the microbial agent for the soil acidification area according to claim 1, wherein the method comprises the following steps: the temperature of high-temperature sterilization in the S2 is 90-130 ℃, the sterilization time is 20-40min, and the mass ratio of the sterilization and impurity removal soil sample to the water in the S3 is 1: 1-2.

3. The method for preparing the microbial agent for the soil acidification area according to claim 1, wherein the method comprises the following steps: the beneficial microorganism strains in the S4 are one or more of lactic acid bacteria, rhizobia, bacillus and yeast, and the volume ratio of the beneficial microorganism, the culture medium and the screening solution is 1: 20-30: 50-100.

4. The method for preparing the microbial agent for the soil acidification area according to claim 1, wherein the method comprises the following steps: the fermented material in the S8 is prepared by uniformly mixing bean flour and bran according to the volume ratio of 1:4, and the volume ratio of the fermented material to the bacterial liquid in the S8 is 1: 2-3.

5. The method for preparing the microbial agent for the soil acidification area according to claim 1, wherein the method comprises the following steps: microorganism collection ball includes hollow spherical shell (101), the bottom of hollow spherical shell (101) is inlayed and is installed accuse water pellicle (102), chemotaxis ball (103) that two symmetries of fixedly connected with set up on the inner wall of hollow spherical shell (101), the middle part of hollow spherical shell (101) is provided with elasticity gasbag (104), equal fixedly connected with gangbar (106) on the outer wall of elasticity gasbag (104) both sides, the through-hole has all been seted up on the outer wall of hollow spherical shell (101) both sides, two the equal fixedly connected with of one end and through-hole assorted hole stopper (107), two of elasticity gasbag (104) are kept away from in gangbar (106) hole stopper (107) are pegged graft with two through-holes respectively.

6. The method for preparing the microbial agent for the soil acidification area according to claim 5, wherein the method comprises the following steps: the inner wall of elasticity gasbag (104) is provided with design net (105), discharge hole (108) have been seted up on the top of hollow spherical shell (101), the middle part of discharge hole (108) runs through and is provided with air duct (109), air duct (109) run through the top outer wall of elasticity gasbag (104), design net (105) in proper order and extend to design net (105), equal fixedly connected with bracing piece (110) on the outer wall of air duct (109) both sides, the inner wall fixed connection of one end and hollow spherical shell (101) of air duct (109) are kept away from in bracing piece (110).

7. The method for preparing the microbial agent for the soil acidification area according to claim 6, wherein the method comprises the following steps: an elastic inflating bag (111) is arranged above the air guide tube (109), the top end of the elastic inflating bag (111) is communicated with an air suction one-way valve (112), the bottom end of the elastic inflating bag (111) is communicated with an air outlet one-way valve (113), and one end, far away from the elastic inflating bag (111), of the air outlet one-way valve (113) is communicated with the air guide tube (109).

8. The method for preparing the microbial agent for the soil acidification area according to claim 7, wherein the method comprises the following steps: be provided with air escape valve (114) on the outer wall of air duct (109) one side, fixed cover is equipped with water conservancy diversion fill (115) on the outer wall of air duct (109), handle (116) are held to two symmetry settings of fixedly connected with on the outer wall of hollow spherical shell (101).

9. The method for preparing the microbial agent for the soil acidification area according to claim 8, wherein the method comprises the following steps: the chemotaxis ball (103) is filled with glucose solution (117), a plurality of villi (118) of adhering to of fixedly connected with on the outer wall of chemotaxis ball (103), chemotaxis ball (103) adopt the semi-permeable membrane material to make, and the membrane pore on chemotaxis ball (103) is less than the glucose molecule.

10. The method for preparing the microbial agent for the soil acidification area according to claim 5, wherein the method comprises the following steps: all be provided with on the inner wall of screening case (002) both sides with hole stopper (107) assorted locating piece (201), after putting microorganism collection ball into screening case (002) in S5, the liquid level height of bacterial screening culture solution is not less than the bottom of hole stopper (107).