CN113214982A

CN113214982A - PCR chamber

Info

Publication number: CN113214982A
Application number: CN202110642134.3A
Authority: CN
Inventors: 唐清泰; 杨海英; 王雷; 张志强
Original assignee: Beijing Applied Biological Technologies Co ltd
Current assignee: Beijing Applied Biological Technologies Co ltd
Priority date: 2021-06-09
Filing date: 2021-06-09
Publication date: 2021-08-06
Anticipated expiration: 2041-06-09
Also published as: CN113214982B

Abstract

The invention provides a PCR chamber, which comprises a lifting door assembly, an upper plate, a side plate and a bottom plate, wherein the lifting door assembly, the upper plate, the bottom plate and the side plate enclose a cavity; the cavity is internally provided with an amplification detection assembly, the amplification detection assembly comprises a PCR container fixing plate, a temperature control plate and a linear guide rail, the PCR container fixing plate is arranged on the upper side of the temperature control plate, the temperature control plate is in sliding connection with the linear guide rail, and the PCR container fixing plate and the temperature control plate are configured to penetrate through the hollow part to the outer side of the cavity. The invention has the characteristics of reagent pollution prevention and convenient operation.

Description

PCR chamber

Technical Field

The invention relates to the technical field of polymerase chain reaction, in particular to a PCR chamber.

Background

Document CN112812959A discloses a nucleic acid extraction, amplification and detection integrated device, including the card box and all set up in the schizolysis liquid chamber of card box, the washing liquid chamber, the eluent chamber, the magnetic bead chamber, combine the liquid chamber, the waste liquid chamber, freeze-drying bead chamber, PCR chamber and sample processing chamber, wherein, combine the liquid chamber can communicate in the magnetic bead chamber, sample processing chamber can be selective intercommunication schizolysis liquid chamber, washing liquid chamber, the eluent chamber, the magnetic bead chamber, one in waste liquid chamber and the freeze-drying bead chamber, freeze-drying bead chamber can communicate in the PCR chamber. Through above-mentioned each cavity, can realize extracting, amplifying, the automated processing who detects nucleic acid, the testing result is more accurate, and only needs a small amount of manual operation steps, easy operation, and is safe convenient. However, the above-mentioned technical solutions install each chamber on the cartridge, and cannot prevent cross contamination inside the device.

Document CN110331090A discloses a nucleic acid extraction, amplification and detection device, which includes a base, an extraction component and an amplification detection component, wherein the extraction component includes a reagent rack detachably mounted on the base, and a liquid suction mechanism for sucking a reagent, the reagent rack includes a first body, and a reaction cavity and a plurality of reagent cavities arranged on the first body, the reagent cavities respectively contain an extraction reagent and a reaction reagent, and the liquid suction mechanism respectively sucks a sample, the extraction reagent and the reaction reagent and transfers the sucked sample and reagent into the reaction cavity; the amplification detection assembly comprises an amplification detection chamber, and the amplification detection chamber is arranged on the base; the amplification detection chamber contains a reaction cavity for amplifying, detecting and analyzing the extracted sample. The reagent rack of the device can be detached and replaced, and different samples can be analyzed quickly; the operation process of nucleic acid extraction and the structure of the extraction assembly are simplified, and the cost of the device and the operation is reduced; the integrated arrangement is adopted, the structure is compact, the volume is small, and the carrying is convenient. However, the above technical solution still requires to manually place the reaction cavity in the accommodating portion of the amplification detection chamber, which easily causes reagent contamination and affects the PCR experimental result.

Disclosure of Invention

Aiming at the problem that reagents in the prior art are easy to be polluted, the invention provides a PCR chamber.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows: a PCR chamber comprises a lifting door assembly, an upper plate, a side plate and a bottom plate, wherein the lifting door assembly, the upper plate, the bottom plate and the side plate enclose a cavity;

the cavity is internally provided with an amplification detection assembly, the amplification detection assembly comprises a PCR container fixing plate, a temperature control plate and a linear guide rail, the PCR container fixing plate is arranged on the upper side of the temperature control plate, the temperature control plate is in sliding connection with the linear guide rail, and the PCR container fixing plate and the temperature control plate are configured to penetrate through the hollow part to the outer side of the cavity.

The amplification detection assembly further comprises a scanning module for collecting real-time fluorescence signal data, and the scanning module is arranged on the lower side of the temperature control plate.

One side, close to the hollow-out part, of the temperature control panel is provided with a connecting hole, and a buckle is arranged on the inner side of the connecting hole.

The amplification detection assembly further comprises an electromagnet module, the electromagnet module is connected with the buckle, and the buckle is made of a magnetic conductive material.

The temperature control board comprises Peltier modules which are uniformly distributed on the temperature control board.

The PCR chamber further comprises a filter assembly disposed on one side of the cavity.

The lifting door assembly further comprises a driving motor, a transmission rod, a gear and a rack, wherein the driving motor and the transmission rod are assembled on one side of the cavity of the fixed door plate, the rack is fixed on one side of the cavity of the movable door plate along the vertical direction, the driving motor is in transmission connection with the transmission rod, the transmission rod is fixedly connected with a gear shaft of the gear, and the gear is meshed with the rack.

The lifting door assembly further comprises fixing plates and limiting plates, the fixing plates are fixed on the side, far away from the cavity, of the fixed door plate, the two fixing plates are arranged on two sides of the movable door plate, the limiting plates are connected to the two fixing plates, and a gap for accommodating the movable door plate is reserved between the limiting plates and the fixed door plate.

The lifting door assembly further comprises an air curtain machine, the air curtain machine is fixedly connected with the fixed door plate and is far away from the cavity one side, the air curtain machine is arranged on the upper edge of the hollow part, and the air curtain direction of the air curtain machine is from top to bottom and the width of the air curtain machine is not smaller than the width of the hollow part.

And the air outlet of the air curtain machine deviates to one side far away from the hollow part.

The invention has the following beneficial effects: enclose into the cavity through lift gate subassembly, upper plate, bottom plate and curb plate to set up the amplification detection subassembly inside the cavity, prevent that external operation from polluting the reagent in the amplification or the testing operation in the amplification detection step process. The PCR container fixing plate and the temperature control plate slide on the linear guide rail and then penetrate through the hollow part to the outer side or the inner side of the cavity, so that the operation is convenient.

Drawings

FIG. 1 is a schematic diagram of the overall structure of one embodiment of a PCR chamber.

FIG. 2 is a schematic view showing the overall structure of another state of the PCR chamber shown in FIG. 1.

FIG. 3 is an exploded view of the PCR chamber shown in FIG. 1.

FIG. 4 is a schematic view of the internal structure of the PCR chamber shown in FIG. 1.

FIG. 5 is a schematic diagram of the construction of the lift gate assembly of the PCR chamber shown in FIG. 1.

FIG. 6 is a schematic diagram of the overall structure of another embodiment of the PCR chamber.

In the figure: 1. a lift gate assembly; 101. a movable door panel; 102. fixing the door plate; 103. a fixing plate; 104. a limiting plate; 105. A drive motor; 106. a transmission belt; 107. a transmission rod; 108. a gear; 109. a rack 109; 110. accommodating grooves; 201. Fixing a PCR container; 202. a temperature control panel; 203. connecting holes; 204. an electromagnet module; 205. a linear guide rail; 206. a guide rail fixing member; 3. an upper plate; 301. a power connection module; 302. a power supply module; 4. a side plate; 5. a filter assembly; 501. a filter screen; 6. a base plate; 7. an air curtain machine; 701. an air inlet; 702. and (7) air outlet.

Detailed Description

The invention will be further described with reference to the following detailed description of the drawings.

It should be noted that, without conflict, any combination of the various embodiments or technical features described below may form a new embodiment.

It is to be understood that the terms "center," "upper," "lower," "horizontal," "left," "right," "lateral," "longitudinal," and the like are used in the orientations and positional relationships indicated in the drawings for the convenience of describing the present invention and for the simplicity of description, and do not indicate or imply that the referenced devices or elements must be in a particular orientation, constructed and operated in a particular orientation, and thus are not to be considered as limiting the present invention.

The first embodiment is as follows:

as shown in fig. 1 to 5, a PCR chamber includes a lifting door assembly 1, an upper plate 3, a side plate 4, and a bottom plate 6, where the lifting door assembly 1, the upper plate 3, the bottom plate 6, and the side plate 4 enclose a cavity, and is characterized in that the lifting door assembly 1 is disposed at a side of the cavity, the lifting door assembly 1 includes a movable door plate 101 and a fixed door plate 102, the movable door plate 101 is configured to be capable of moving up and down, a hollow portion is disposed on the fixed door plate 102, and a size of the movable door plate 101 is larger than a size of the hollow portion;

the cavity is internally provided with an amplification detection assembly, the amplification detection assembly comprises a PCR container fixing plate 201, a temperature control plate 202 and a linear guide rail 205, the PCR container fixing plate 201 is arranged on the upper side of the temperature control plate 202, the temperature control plate 202 is connected with the linear guide rail 205 in a sliding mode, and the PCR container fixing plate 201 and the temperature control plate 202 are configured to be capable of penetrating through the hollow part to the outer side of the cavity.

In this embodiment, the amplification detecting assembly further comprises a rail fixing member 206, and the rail fixing member 206 is disposed between the linear rail 205 and the temperature control plate 202 to prevent friction caused by sliding between the linear rail 205 and the temperature control plate 202 from affecting the temperature of the temperature control plate 202 and prevent abrasion of the temperature control plate 202.

Description of the principles of the invention: a cavity is defined by the lifting door assembly 1, the upper plate 3, the bottom plate 6 and the side plate 4, and the amplification detection assembly is arranged in the cavity, so that the amplification or detection operation reagent is prevented from being polluted by external operation in the amplification detection step; meanwhile, when the nucleic acid extraction operation is required, the movable door plate 101 is controlled to move so as to open the hollow part, so that the PCR container fixing plate 201 and the temperature control plate 202 slide on the linear guide rail 205 and further penetrate through the hollow part to the outer side of the cavity, so that the nucleic acid extraction operation is performed; after finishing nucleic acid extraction, make PCR container fixed plate 201, temperature control board 202 pass the fretwork portion and get into inside the cavity, control movable door panel 101 afterwards and remove in order to seal the fretwork portion, make the cavity seal to prevent that operations such as nucleic acid extraction outside the cavity from causing the pollution to the reagent of inboard amplification detection operation involving, convenient operation can reduce the working strength of PCR in-process.

The amplification detection assembly further comprises a scanning module for real-time fluorescence signal data acquisition, the scanning module being disposed under the temperature control plate 202.

As shown in fig. 1 and 3, a connection hole 203 is formed on one side of the temperature control plate 202 close to the hollow portion, and a buckle is arranged on the inner side of the connection hole 203. When the temperature control plate 202 and the PCR vessel fixing plate 201 need to be moved out of the cavity, the connecting piece is clamped into the buckle, so that the temperature control plate 202 and the PCR vessel fixing plate 201 can be conveniently moved out of the cavity. In addition, based on the concept of the present invention, an exposed detachable connection structure, such as a snap, a hook, etc., may be further provided to facilitate the connection and control of the temperature control board 202.

As shown in fig. 1 and 3, the amplification detection assembly further includes an electromagnet module 204, the electromagnet module 204 is connected to the buckle, and the buckle is made of a magnetic material. When the connecting piece stretches into the connecting hole 203 and is clamped with the fastener, the electromagnet module 204 is electrified to generate a magnetic field and transmit the magnetic field to the fastener, and when the connecting piece is made of metal or other magnetizers, the connecting piece is adsorbed on the fastener through the magnetic field of the fastener, so that the connection reliability is further improved. Based on the conception of the invention, the buckle can also be directly arranged as an electromagnet, or the connecting piece is arranged as an electromagnet, and the buckle is arranged as a magnetizer, so as to improve the reliability of connection.

The temperature control plate 202 includes peltier modules that are evenly distributed across the temperature control plate 202. The temperature of the temperature control plate 202 is controlled by the peltier module, thereby controlling the temperature of the PCR reagents therein.

As shown in fig. 4, the PCR chamber further comprises a filter assembly 5, wherein the filter assembly 5 is disposed on one side of the cavity. By the filtering component 5, harmful substances in the gas in the cavity are prevented from being dissipated to the external environment, and the pollution of the external environment is prevented.

As shown in fig. 4, a filter screen 501 is further disposed on a side of the filter assembly 5 away from the cavity, so as to filter the gas discharged from the filter assembly 5.

As shown in fig. 5, the lift door assembly 1 further includes a driving motor 105, a transmission rod 107, a gear 108, and a rack 109, wherein the driving motor 105 and the transmission rod 107 are assembled on one side of the cavity of the fixed door panel 102, the rack 109 is fixed on one side of the cavity of the movable door panel 101 along a vertical direction, the driving motor 105 is in transmission connection with the transmission rod 107, the transmission rod 107 is fixedly connected with a gear shaft of the gear 108, and the gear 108 is engaged with the rack 109. In this embodiment, in order to ensure that the rack 109 can operate effectively and avoid friction between the rack 109 and the fixed door plate 102, an accommodating groove 110 is provided at a position of the fixed door plate 102 corresponding to the rack 109. In this embodiment, the driving connection between the driving motor 105 and the transmission rod 107 is performed by a belt driving manner, as shown in fig. 5, a pulley is fixed on the rotating shaft of the driving motor 105 and the transmission rod 107 respectively, and a driving belt 106 is sleeved outside the pulley.

As shown in fig. 5, the lifting door assembly 1 further includes a fixing plate 103 and a limiting plate 104, the fixing plate 103 is fixed to one side of the fixing door panel 102, which is far away from the cavity, two fixing plates 103 are disposed on two sides of the movable door panel 101, the two fixing plates 103 are connected with the limiting plate 104, and a gap for accommodating the movable door panel 101 is reserved between the limiting plate 104 and the fixing door panel 102.

Example two:

as shown in fig. 6, on the basis of the first embodiment, the lifting door assembly 1 further includes an air curtain machine 7, the air curtain machine 7 is fixedly connected to the fixed door plate 102 far away from one side of the cavity, the air curtain machine 7 is disposed at the upper edge of the hollow portion, and the air curtain direction of the air curtain machine 7 is from top to bottom and the width is not smaller than the width of the hollow portion. Because the temperature control plate 202 is arranged in the cavity, the gas temperature in the cavity and the gas temperature outside the wall body are different, and further the difference of the gas pressure is caused. When there is a difference in air pressure between the inside and the outside of the cavity, the movable door plate 101 moves and opens the cavity, and at this time, due to the difference in air pressure between the inside and the outside of the cavity, the gas inside and the outside of the cavity will flow and exchange rapidly, thereby greatly increasing the probability of reagent contamination. Through setting up air curtain machine 7 and producing the air curtain for movable door panel 101 removes and when making the cavity open, the gas exchange inside and outside the cavity that significantly reduces, thereby prevents that reagent from receiving the pollution.

As shown in fig. 6, the air outlet 702 of the air curtain machine 7 is shifted to a side away from the hollow portion, so that when the cavity is opened, the air curtain does not blow into the cavity, thereby preventing the reagent in the cavity from being polluted.

Finally, it should be noted that the above-mentioned contents are only used for illustrating the technical solutions of the present invention, and not for limiting the protection scope of the present invention, and that the simple modifications or equivalent substitutions of the technical solutions of the present invention by those of ordinary skill in the art can be made without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. The utility model provides a PCR chamber, includes lift door subassembly, upper plate, curb plate, bottom plate, lift door subassembly, upper plate, bottom plate and curb plate enclose into cavity, its characterized in that: the lifting door assembly is arranged on the side face of the cavity and comprises a movable door plate and a fixed door plate, the movable door plate is configured to be capable of moving up and down, a hollow part is arranged on the fixed door plate, and the size of the movable door plate is larger than that of the hollow part;

2. The PCR chamber of claim 1, wherein: the amplification detection assembly further comprises a scanning module for collecting real-time fluorescence signal data, and the scanning module is arranged on the lower side of the temperature control plate.

3. The PCR chamber of claim 1, wherein: one side, close to the hollow-out part, of the temperature control panel is provided with a connecting hole, and a buckle is arranged on the inner side of the connecting hole.

4. The PCR chamber of claim 3, wherein: the amplification detection assembly further comprises an electromagnet module, the electromagnet module is connected with the buckle, and the buckle is made of a magnetic conductive material.

5. The PCR chamber of claim 1, wherein: the temperature control board comprises Peltier modules which are uniformly distributed on the temperature control board.

6. The PCR chamber of claim 1, wherein: the PCR chamber further comprises a filter assembly disposed on one side of the cavity.

7. The PCR chamber of claim 1, wherein: the lifting door assembly further comprises a driving motor, a transmission rod, a gear and a rack, wherein the driving motor and the transmission rod are assembled on one side of the cavity of the fixed door plate, the rack is fixed on one side of the cavity of the movable door plate along the vertical direction, the driving motor is in transmission connection with the transmission rod, the transmission rod is fixedly connected with a gear shaft of the gear, and the gear is meshed with the rack.

8. The PCR chamber of claim 1, wherein: the lifting door assembly further comprises fixing plates and limiting plates, the fixing plates are fixed on the side, far away from the cavity, of the fixed door plate, the two fixing plates are arranged on two sides of the movable door plate, the limiting plates are connected to the two fixing plates, and a gap for accommodating the movable door plate is reserved between the limiting plates and the fixed door plate.

9. The PCR chamber of claim 1, wherein: the lifting door assembly further comprises an air curtain machine, the air curtain machine is fixedly connected with the fixed door plate and is far away from the cavity one side, the air curtain machine is arranged on the upper edge of the hollow part, and the air curtain direction of the air curtain machine is from top to bottom and the width of the air curtain machine is not smaller than the width of the hollow part.

10. The PCR chamber of claim 9, wherein: and the air outlet of the air curtain machine deviates to one side far away from the hollow part.