CN113208766B - Motion sickness mouse molding device and molding method - Google Patents
Motion sickness mouse molding device and molding method Download PDFInfo
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Abstract
The invention relates to a motion sickness mouse molding device and a molding method, wherein the molding device comprises a rail, a sensor, a trolley, a mouse cage, a display screen, a camera, an infrared thermometer and a remote control panel, the trolley is arranged on the rail in a sliding manner, the rail comprises a straight part and a bent part, the straight part and the bent part are connected end to form a ring, the sensor is arranged on the outer side of the straight part and used for sensing the passing of the trolley to control the advancing speed of the trolley, the mouse cage is arranged on the trolley, the display screen, the camera and the infrared thermometer are arranged in the mouse cage, and the display screen, the camera and the infrared thermometer are all electrically connected with the remote control panel. The mouse motion sickness modeling device is controlled by matching the track with the sensor to move, the process of accelerating and decelerating vehicles causing motion sickness in daily life is well simulated, the display screen is arranged, the eyes of a mouse can directly view the display screen, animation played by the display screen can further stimulate the mouse to generate motion sickness, the mouse motion sickness modeling device is evaluated through a plurality of indexes, and the verification accuracy is improved.
Description
Technical Field
The invention relates to the technical field of animal experiments, in particular to a motion sickness mouse molding device and a molding method.
Background
Motion sickness (motion sickness), which is a general term for motion sickness, and diseases caused by rolling, jolting, rotating, accelerating movements, etc., due to various causes. Motion sickness occurs because the brain receives conflicting information from the sense organs: the eye is unable to identify the mechanism (relationship) by which the movement of the same control and the movement of the vehicle create a balance in the inner ear. The central nervous system responds to this stress by nausea and central activity in the brain, with motion sickness affecting the efficiency of daily work and the quality of travel.
Establishing a motion sickness model is very important for researching pathogenesis, in recent years, experimenters try to induce motion sickness through animal experiments, and CN202043526U discloses a mouse motion sickness modeling device which comprises a base, a rotating motor arranged in the base, a rotating vertical shaft connected with the rotating motor, a rotating transverse shaft vertically connected with the rotating vertical shaft, and mouse cages respectively connected with two ends of the rotating transverse shaft, wherein the center position of the rotating transverse shaft is vertically connected with the rotating vertical shaft, and the rotating motor is connected with a rotating speed adjusting device. CN201375509Y discloses a rotary speed-regulating type rat motion sickness inducing instrument, which comprises a large disc and a small disc fixed on the large disc; the large disc and the small disc are respectively connected with a driving motor of the large disc and a driving motor shaft of the small disc; the driving motor of the large disk and the driving motor of the small disk are electrically connected with the control circuit. In summary, these devices have complicated structures, cannot simulate the actual environmental scene well, and bring certain obstacles to research, so it is necessary to develop a molding device and a molding method for mice with motion sickness, which are easy and convenient to operate, have good simulation effect, and have high success rate of induction.
Disclosure of Invention
In order to solve the technical problems, the invention provides a motion sickness mouse modeling device which comprises a rail, a sensor, a trolley, a mouse cage, a display screen, a camera, an infrared thermometer and a remote control panel, wherein the trolley is arranged on the rail in a sliding mode, the rail comprises a linear part and a bent part, the linear part and the bent part are connected end to form a ring, the sensor is arranged on the outer side of the linear part and used for sensing the passing of the trolley to control the advancing speed of the trolley, the mouse cage is arranged on the trolley, the display screen, the camera and the infrared thermometer are arranged in the mouse cage, and the display screen, the camera and the infrared thermometer are electrically connected with the remote control panel.
In one embodiment of the present invention, the sensor includes a first sensor and a second sensor, which are respectively disposed at both ends of the linear portion.
In one embodiment of the invention, the first and second sensors are photosensors.
In one embodiment of the invention, the number of the display screens is multiple, and the display screens are arranged on the inner side of the squirrel cage and surround the squirrel cage.
In one embodiment of the invention, the display screen continuously plays animated video.
In one embodiment of the invention, the remote control panel comprises three keys, and the three keys respectively control the trolley, the camera and the infrared thermometer.
In one embodiment of the invention, the remote control panel comprises a liquid crystal screen for displaying real-time images of the mouse in the mouse cage.
In addition, the invention also provides a mouse molding method for motion sickness, which comprises the following steps:
step one, a mouse is placed in a mouse cage;
opening an infrared thermometer, a display screen and a camera through a remote control panel, starting to measure the body temperature of the mouse by the infrared thermometer, continuously playing animation by the display screen, and recording the behavior of the mouse in a mouse cage by the camera;
thirdly, starting the trolley through the remote control panel to enable the trolley to advance on the track, accelerating the trolley when the trolley advances to a first sensor of one straight line part, then starting to decelerate when the trolley advances to a second sensor of the straight line part, slowly passing through one bending part, then advancing to a first sensor of the other straight line part again, accelerating the trolley, then starting to decelerate when the trolley advances to a second sensor of the straight line part, slowly passing through the other bending part, and automatically braking and stopping the trolley after one circle is finished;
and step four, recording the body temperature and the behavior of the mouse in the process of the step three, and analyzing to obtain the motion sickness modeling effect.
In one embodiment of the invention, the behaviors include piloerection behavior and conditional mouth opening behavior.
In one embodiment of the present invention, the mice are 6-week-old healthy male mice, and the breeding environment of the mice is: the temperature is 23 +/-3 ℃ and the humidity is 55 +/-15 percent, and one mouse is fed in each cage.
The invention has the beneficial effects that: the device controls the trolley to advance by matching the annular track with the sensor, better simulates the acceleration and deceleration process of a vehicle causing motion sickness in daily life, and is also provided with the display screen, so that the eyes of a mouse can look directly at the display screen, animation played by the display screen can further stimulate the mouse to generate the motion sickness, and the mouse motion sickness modeling device is evaluated by a plurality of indexes to improve the verification accuracy, so that the device has high success rate of inducing the motion sickness, is simple in structure, convenient and fast to operate, easy to popularize and apply, and can provide a certain thought for the pathogenesis of the motion sickness and the drug research.
Drawings
In order that the present disclosure may be more readily and clearly understood, reference will now be made in detail to the present disclosure, examples of which are illustrated in the accompanying drawings.
Fig. 1 is a schematic structural diagram of a molding apparatus according to a first embodiment of the present invention.
Fig. 2 is a schematic structural diagram of a track according to an embodiment of the present invention.
Fig. 3 is a schematic diagram of the distribution of the display screen in the squirrel cage according to the first embodiment of the invention.
FIG. 4 shows the data of the average body temperature of each group of mice measured by an infrared thermometer.
FIG. 5 is the average number of hair setting and conditional mouth opening behavior data for each group.
The specification reference numbers indicate: 100. a track; 110. a straight portion; 120. a bending section; 210. a first sensor; 220. a second sensor; 300. a trolley; 400. a mouse cage; 500. a display screen; 600. a camera; 700. an infrared thermometer.
Examples
The present invention is further described below in conjunction with the following figures and specific examples so that those skilled in the art may better understand the present invention and practice it, but the examples are not intended to limit the present invention.
The invention comprises a test group and a control group, wherein the test group comprises a test group A, a test group B, a test group C and a control group.
Test animals: each experimental group comprises 50 mice, the mice are 6-week-old healthy male C57BL/6J mice, and the breeding environment of the mice is as follows: the temperature is 23 +/-3 ℃ and the humidity is 55 +/-15 percent, and one mouse is fed in each cage.
Test group a: as shown in fig. 1-3, a molding device for a mouse with motion sickness comprises a track 100, a sensor, a trolley 300, a squirrel cage 400, a display screen 500, a camera 600, an infrared thermometer 700 and a remote control panel. The track 100 is arranged on the ground, the track 100 is composed of two linear portions 110 and two bending portions 120, the linear portions 110 and the bending portions 120 are connected end to form a ring structure, sensors are arranged on the outer sides of the linear portions 110 of the track 100, the sensors include a first sensor 210 and a second sensor 220, the first sensor 210 and the second sensor 220 can be photoelectric sensors, the first sensor 210 and the second sensor 220 are respectively located at two ends of the linear portions 110, and the first sensor 210 and the second sensor 220 sense the passing of the trolley 300 so as to control the traveling speed of the trolley 300. The cart 300 has four wheels at the bottom that mate with the track 100. The squirrel cage 400 is fixedly connected with the trolley 300 and is positioned in the trolley 300, and the squirrel cage 400 is a rectangular cage. The display screen 500 is six in number, the display screen 500 is located on the inner side of the squirrel cage 400 and arranged around the squirrel cage 400, and the display screen 500 continuously plays animation videos. The camera 600 is installed in the squirrel cage 400 to photograph the whole experimental process. The infrared thermometer 700 is arranged in the squirrel cage 400 and is used for measuring the body temperature of the mouse. The remote control panel is electrically connected with the trolley 300, the camera 600 and the infrared thermometer 700, the remote control panel comprises three keys and a liquid crystal screen, the three keys respectively control the trolley 300, the camera 600 and the infrared thermometer 700, and the liquid crystal screen is used for displaying real-time images of a mouse in the squirrel cage 400.
The molding method using the molding device comprises the following steps: step one, placing mice into a mouse cage 400, wherein one mouse is placed in each cage; opening the infrared thermometer 700, the display screen 500 and the camera 600 through the remote control panel, starting to measure the body temperature of the mouse by the infrared thermometer 700, continuously playing animation by the display screen 500, and recording the behavior of the mouse in the squirrel cage by the camera 600; step three, starting the trolley 300 through the remote control panel, enabling the trolley 300 to travel on the track 100, when the trolley 300 travels to the first sensor 210 of one straight part 110, accelerating the trolley 300, then the trolley 300 travels to the second sensor 220 of the straight part 110 to start decelerating and slowly pass through one bending part 120, then the trolley 300 travels to the first sensor 210 of the other straight part 110 again, accelerating the trolley 300, then the trolley 300 travels to the second sensor 220 of the straight part 110 to start decelerating and slowly pass through the other bending part 120, and after one circle is completed, the trolley 300 automatically brakes and stops; and step four, recording the body temperature and the behaviors of the mouse in the process of the step three, and analyzing to obtain the motion sickness modeling effect, wherein the behaviors comprise piloerection behaviors and conditional mouth opening behaviors.
Test group B: the only difference between the same molding apparatus as test group a and the molding method of test group a is that the cart 300 is stationary and has no acceleration/deceleration process. The molding method comprises the following steps: step one, placing mice into a mouse cage 400, wherein one mouse is placed in each cage; step two, the infrared thermometer 700, the display screen 500 and the camera 600 are opened through the remote control panel, the infrared thermometer 700 starts to measure the body temperature of the mouse, the display screen 500 continuously plays animation, and the camera 600 records the behavior of the mouse in the squirrel cage 400; step three, the trolley 300 is stationary, and the stationary time is the same as that of the trolley 300 circling one circle in the test A. And step four, recording the body temperature and the behavior of the mouse in the process of the step three, and analyzing to obtain the motion sickness modeling effect. Behaviors include piloerection behavior and conditional mouth opening behavior.
Test group C: the only difference from the molding apparatus of test group a is that the display screen 500 is not included in the molding apparatus of this group. The molding method comprises the following steps: step one, placing mice into a mouse cage 400, wherein one mouse is placed in each cage; step two, the infrared thermometer 700 and the camera 600 are opened through the remote control panel, the infrared thermometer 700 starts to measure the body temperature of the mouse, and the camera 600 records the behavior of the mouse in the squirrel cage 400; step three, starting the trolley 300 through the remote control panel, enabling the trolley 300 to travel on the track 100, when the trolley 300 travels to the first sensor 210 of one straight part 110, accelerating the trolley 300, then the trolley 300 travels to the second sensor 220 of the straight part 110 to start decelerating and slowly pass through one bending part 120, then the trolley 300 travels to the first sensor 210 of the other straight part 110 again, accelerating the trolley 300, then the trolley 300 travels to the second sensor 220 of the straight part 110 to start decelerating and slowly pass through the other bending part 120, and after one circle is completed, the trolley 300 automatically brakes and stops; and step four, recording the body temperature and the behaviors of the mouse in the process of the step three, and analyzing to obtain a motion sickness modeling effect, wherein the behaviors comprise piloerection behaviors and conditional mouth opening behaviors.
Control group: the only difference from the molding apparatus of test group a is that the display screen 500 is not included in the molding apparatus of this group. The molding method comprises the following steps: step one, placing mice into a mouse cage 400, wherein one mouse is placed in each cage; step two, the infrared thermometer 700 and the camera 600 are opened through the remote control panel, the infrared thermometer 700 starts to measure the body temperature of the mouse, and the camera 600 records the behavior of the mouse in the squirrel cage 400; step three, the vehicle is stationary, and the stationary time is the same as the time when the trolley 300 circles for one circle in the test A; and step four, recording the body temperature and the behaviors of the mouse in the process of the step three, and analyzing to obtain the motion sickness modeling effect, wherein the behaviors comprise piloerection behaviors and conditional mouth opening behaviors.
The temperature and behavior of the mice were recorded by using the molding apparatus and molding method of each group, and the obtained test data are shown in fig. 4 and 5.
As can be seen from fig. 4, the body temperature of the control group, i.e., normal mice, was 36.5 ℃, the average body temperature of the three test groups was less than that of the control group, and the body temperature of test group a was 35.9 ℃, which was the lowest temperature of the three test groups, while the temperatures of test group B and test group C were close, 36.3 ℃ and 36.2 ℃, respectively.
It can be seen from fig. 5 that the hair setting times/hair and the conditional mouth opening times/hair of each test group are significantly higher than those of the control group, wherein the hair setting times/hair and the conditional mouth opening times/hair of the test group A are the highest, and the data of the test group B and the test group C are close.
Various research data show that the mice have the body temperature drop and generate the phenomena of hair erection and conditional mouth opening when the mice generate the motion sickness, and the data in figures 4-5 prove that the three mouse motion sickness modeling devices and methods can stimulate the mice to generate the motion sickness, and the modeling effect of the modeling devices and methods used in the test group A is the best in comparison.
It should be understood that the above examples are only for clarity of illustration and are not intended to limit the embodiments. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications of the invention may be made without departing from the spirit or scope of the invention.
Claims (1)
1. A method for molding a mouse with motion sickness utilizes a device for molding the mouse with motion sickness, and is characterized in that: the molding device comprises a rail, a sensor, a trolley, a mouse cage, a display screen, a camera, an infrared thermometer and a remote control panel, wherein the trolley is arranged on the rail in a sliding manner, the rail comprises a linear part and a bent part, the linear part and the bent part are connected end to form a ring, the sensor is arranged on the outer side of the linear part, the sensor senses the passing of the trolley to control the traveling speed of the trolley, the mouse cage is arranged on the trolley, the display screen, the camera and the infrared thermometer are arranged in the mouse cage, and the display screen, the camera and the infrared thermometer are all electrically connected with the remote control panel; the sensor comprises a first sensor and a second sensor, and the first sensor and the second sensor are respectively arranged at two ends of the straight line part; the first sensor and the second sensor are photoelectric sensors; the mouse cage comprises a mouse cage body, a mouse cage and a plurality of display screens, wherein the mouse cage body is provided with a mouse cage; the display screen continuously plays animation videos; the remote control panel comprises three keys, and the three keys respectively control the trolley, the camera and the infrared thermometer; the remote control panel comprises a liquid crystal screen, and the liquid crystal screen is used for displaying real-time images of the mouse in the mouse cage;
the molding method specifically comprises the following steps:
step one, a mouse is placed in a mouse cage;
opening an infrared thermometer, a display screen and a camera through a remote control panel, starting to measure the body temperature of the mouse by the infrared thermometer, continuously playing animation by the display screen, and recording the behavior of the mouse in a mouse cage by the camera;
thirdly, starting the trolley through the remote control panel to enable the trolley to advance on the track, accelerating the trolley when the trolley advances to a first sensor of one straight line part, then starting to decelerate when the trolley advances to a second sensor of the straight line part, slowly passing through one bending part, then advancing to a first sensor of the other straight line part again, accelerating the trolley, then starting to decelerate when the trolley advances to a second sensor of the straight line part, slowly passing through the other bending part, and automatically braking and stopping the trolley after one circle is finished;
recording the body temperature and the behavior of the mouse in the third step, and analyzing to obtain the motion sickness modeling effect; the behaviors include piloerection behavior and conditional mouth opening behavior; the mice are male mice with 6 weeks of age and healthy bodies, and the breeding environment of the mice is as follows: the temperature is 23 +/-3 ℃ and the humidity is 55 +/-15 percent, and one mouse is fed in each cage.
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