CN113150947A - Vibration device for exosome extraction - Google Patents
Vibration device for exosome extraction Download PDFInfo
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- CN113150947A CN113150947A CN202110532774.9A CN202110532774A CN113150947A CN 113150947 A CN113150947 A CN 113150947A CN 202110532774 A CN202110532774 A CN 202110532774A CN 113150947 A CN113150947 A CN 113150947A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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Abstract
The invention relates to the technical field of exosomes, in particular to an oscillating device for exosome extraction, wherein built-in grooves are symmetrically arranged on two sides of the inner end of a shell of the oscillating device in a penetrating way, wherein a rectangular sliding block is arranged in the built-in groove, a bottom plate connected with a screw is arranged at the lower side of the inner end of the device shell, wherein one side of the bottom plate is provided with a first motor shaft connected with a rotating shaft in a penetrating way, the upper end of the first motor shaft is provided with a fixed rotating disc, wherein one side of the upper end of the rotating disc is provided with a linkage rod which is hinged, the middle end of the end cover is provided with a first holding groove in a penetrating way, wherein the upper side of the middle end of the end cover is provided with a circular cylinder which is welded and connected, the middle end of the circular cylinder is provided with a second motor shaft which is connected with a rotating shaft in a penetrating way, the upper end of the second motor shaft is provided with a fixedly connected motor, the side of the end cover is uniformly penetrated and provided with a second accommodating groove, and an elastic plate is placed on one side of the inner end of the second accommodating groove.
Description
Technical Field
The invention relates to the technical field of exosomes, in particular to an oscillating device for exosome extraction.
Background
Exosomes refer to small membrane vesicles (30-150nm) containing complex RNAs and proteins, which today refer specifically to discoidal vesicles with diameters between 40-100 nm. In 1983, exosomes were first found in sheep reticulocytes, which were named "exosomes" by Johnstone in 1987. Many cells secrete exosomes under both normal and pathological conditions. It is mainly derived from multivesicular bodies formed by invagination of intracellular lysosome particles, and is released into extracellular matrix after fusion of multivesicular body outer membranes and cell membranes, all cultured cell types can secrete exosomes, and the exosomes naturally exist in body fluid, including blood, saliva, urine, cerebrospinal fluid and milk. The precise molecular mechanisms involved in their secretion and uptake and their composition, "cargo" and corresponding functions have just begun to be studied. Exosomes are currently considered to be specifically secreted membrane vesicles, involved in intercellular communication, and increasingly interested in exosome research, whether for studying their function or for understanding how they are used in the development of minimally invasive diagnostics.
At present, need the staff to carry out manual shock to it at the exosome extraction in-process, and then this kind of mode can increase staff's fatigue, and manual shock exosome exists to vibrate not thoroughly simultaneously, the time spent overlength with can not vibrate the operation scheduling problem to many exosomes simultaneously, if set up the vibration device that an exosome extracted the usefulness this moment, then can effectively solve the problem that above-mentioned in-process mentioned.
Disclosure of Invention
The present invention is directed to an oscillation device for exosome extraction to solve the above-mentioned problems.
In order to achieve the purpose, the invention provides the following technical scheme: an oscillating device for extracting exosome, wherein built-in grooves are symmetrically arranged on two sides of the inner end of a device shell in a penetrating way, a rectangular sliding block is arranged in each built-in groove, a bottom plate connected with a screw is arranged on the lower side of the inner end of the device shell, a first motor shaft connected with a rotating shaft is arranged on one side of the bottom plate in a penetrating way, a fixed rotating disc is arranged at the upper end of the first motor shaft, a linkage rod connected with the rotating disc in a hinged way is arranged on one side of the upper end of the rotating disc, a first accommodating groove is arranged at the middle end of an end cover in a penetrating way, a circular cylinder connected with the end cover in a welding way is arranged on the upper side of the middle end of the end cover, a second motor shaft connected with the rotating shaft is arranged at the middle end of the circular cylinder in a penetrating way, a motor connected with the second motor is arranged at the upper end of the second motor shaft, a second accommodating groove is uniformly arranged on the side of the end cover in a penetrating way, an elastic plate is arranged on one side of the inner end of the second accommodating groove, and the elastic plate is connected with the end cover by the screw, wherein elastic plate one end sets up the binding plate of screwed connection, fly leaf upper end one side evenly sets up the fixed cylinder of articulated connection, and wherein the cylinder of placing of gluing connection is placed to fixed cylinder inside, place cylinder inner and place the slipmat of gluing connection, the one end of placing the cylinder wherein runs through the inside setting of second holding tank.
Preferably, the inner end of the end cover penetrates through the first accommodating groove to form a limiting clamping groove, wherein the inner end of the limiting clamping groove penetrates through the pushing rod, one end of the pushing rod is provided with a hemispherical cap in welded connection, the pushing rod is sleeved with a reset spring, and one end of the pushing rod is provided with an arc plate in hinged connection.
Preferably, the hemisphere cap is located inside the first holding tank, wherein the radius of the hemisphere cap is large and the maximum width of the reset spring, one end of the reset spring is located inside the limiting clamping groove, the arc-shaped plate is located inside the second holding tank, and the cylinder is placed in the arc-shaped plate in a fitting mode.
Preferably, the circular cylinder is located the positive upper end of first holding tank, and wherein the groove diameter of first holding tank is greater than the external diameter of placing the drum, the drum setting is placed in the laminating of the one end of rigging board, and wherein the maximum length of rigging board is the same with the internal diameter of first holding tank, the shape that the elastic plate set up is semicircle nature shape, and wherein the material that the elastic plate setting adopted is the spring steel.
Preferably, second motor shaft lower extreme sets up screwed connection's increase apart from cylinder, and wherein increase apart from the cylinder and be located the inside of first holding tank, increase apart from the cylindrical outside and evenly set up half cylinder, wherein the quantity of half cylinder is four, and the angle interval between every half cylinder is ninety degrees, increase apart from the cylindrical length of cylinder and the height of end cover the same.
Preferably, the movable plate is connected with the rectangular sliding block in a welding mode, the movable plate is connected with the linkage rod in a hinged mode, one end, fixed to the movable plate, of the linkage rod is located on the lower side of the middle end of the movable plate, and the rotating distance of the linkage rod on the rotating disc is smaller than the height, exceeding the upper surface of the end cover, of the placing cylinder.
Preferably, the non-slip mat is made of rubber, the thickness of the non-slip mat is kept between three and five millimeters, the length of the non-slip mat is the same as the depth of the groove of the placing cylinder, and the material of the placing cylinder is plastic.
Compared with the prior art, the invention has the beneficial effects that:
1. through the arrangement of the vibration control device, a worker can get rid of the phenomenon of manual vibration of the exosome, so that the work convenience can be brought to the worker, meanwhile, the sufficient vibration of the exosome is facilitated, and the time required by the vibration of the exosome can be shortened;
2. through the setting of placing the drum to different quantity, can make the device vibrate the body that secretes many copies, and then help staff that can be better carries out work, and the inner wall of placing the drum simultaneously sets up the rubber pad, then can protect the household utensils of the body that secretes at the oscillation in-process, and then the effectual condition that prevents the body that secretes and appear leaking.
Drawings
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a schematic perspective view of a half-section of the housing of the apparatus of the present invention;
FIG. 3 is a schematic right side view of a half-section of the housing of the apparatus of the present invention;
FIG. 4 is a schematic semi-sectional perspective view of the end cap and the movable plate of the present invention;
FIG. 5 is an enlarged view taken at A in FIG. 3 according to the present invention;
fig. 6 is an enlarged view of the invention at B in fig. 4.
In the figure: the device comprises a shell 1, a built-in groove 11, a rectangular sliding block 12, a bottom plate 13, a first motor shaft 14, a rotating disc 15, a linkage rod 16, an end cover 2, a limiting clamping groove 201, a push rod 202, a hemispherical cap 203, a reset spring 204, an arc-shaped plate 205, a first accommodating groove 21, a circular cylinder 22, a second motor shaft 23, a distance-increasing cylinder 2301, a semi-cylinder 2302, a motor 24, a second accommodating groove 25, an elastic plate 26, a binding plate 27, a movable plate 3, a fixed cylinder 31, a placing cylinder 32 and an anti-skid pad 33.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1 to 6, the present invention provides a technical solution: comprises a device shell 1, an end cover 2 and a movable plate 3, wherein the end cover 2 is fixed on the upper side of the inner end of the shell 1, the movable plate 3 is positioned in the device shell 1, the inner end of the device shell 1 is symmetrically provided with built-in grooves 11 in a penetrating manner at two sides, rectangular sliders 12 are arranged in the built-in grooves 11, a base plate 13 connected with screws is arranged on the lower side of the inner end of the device shell 1, one side of the base plate 13 is provided with a first motor shaft 14 connected with a rotating shaft in a penetrating manner, a fixed rotating disc 15 is arranged at the upper end of the first motor shaft 14, one side of the upper end of the rotating disc 15 is provided with a linkage rod 16 connected with a hinge joint, the middle end of the end cover 2 is provided with a first accommodating groove 21 in a penetrating manner, the angle between the first accommodating grooves 21 is ninety degrees, the upper side of the middle end of the end cover 2 is provided with a circular cylinder 22 connected with a welding manner, the middle end of the circular cylinder 22 is provided with a second motor shaft 23 connected with a rotating shaft, and the upper end of the second motor shaft 23 is provided with a fixed connection motor 24, the second holding tank 25 is seted up evenly to 2 avris of end cover through, wherein elastic plate 26 is placed to the inner one side of second holding tank 25, carry out screw connection between elastic plate 26 and the end cover 2, wherein elastic plate 26 one end sets up the binding plate 27 of screw connection, movable plate 3 upper end one side evenly sets up the fixed cylinder 31 of articulated connection, wherein fix cylinder 31 inside place the cylinder 32 of placing of glued joint, place cylinder 32 the inner antiskid pad 33 of placing the glued joint, wherein the one end of placing cylinder 32 runs through the inside setting of second holding tank 25.
The inner end of the end cover 2 penetrates through the first accommodating groove 21 and is provided with a limiting clamping groove 201, wherein the inner end of the limiting clamping groove 201 penetrates through and is provided with a push rod 202, one end of the push rod 202 is provided with a hemispherical cap 203 which is welded, a reset spring 204 is sleeved on the push rod 202, and one end of the push rod 202 is provided with an arc-shaped plate 205 which is hinged.
The angle between the half-spherical caps 203 is ninety degrees, wherein the half-spherical cap 203 can push the push rod 202 under the action of the half-cylinder 2302, and at the moment, the push rod 202 reciprocates under the action of the return spring 204.
The hemispherical cap 203 is located inside the first receiving groove 21, wherein the radius of the hemispherical cap 203 is larger than the maximum width of the return spring 204, one end of the return spring 204 is located inside the limiting clamping groove 201, the arc-shaped plate 205 is located inside the second receiving groove 25, and the arc-shaped plate 205 is attached to the placing cylinder 32.
Circular section of thick bamboo 22 is located first holding tank 21 and is just gone up end, and wherein the groove diameter of first holding tank 21 is greater than the external diameter of placing drum 32, and drum 32 setting is placed in the laminating of the one end of rigging board 27, and wherein the maximum length of rigging board 27 is the same with the internal diameter of first holding tank 21, and the shape that elastic plate 26 set up is semicircle nature shape, and wherein the material that elastic plate 26 set up the adoption is the spring steel.
The lower end of second motor shaft 23 sets up the range-increasing cylinder 2301 of screw connection, and wherein range-increasing cylinder 2301 is located the inside of first holding tank 21, and the outside of range-increasing cylinder 2301 evenly sets up half cylinder 2302, and wherein the quantity of half cylinder 2302 is four, and the angular separation between each half cylinder 2302 is ninety degrees, and the length of range-increasing cylinder 2301 is the same with the height of end cover 2.
The movable plate 3 is connected with the rectangular sliding block 12 in a welding mode, the movable plate 3 is connected with the linkage rod 16 in a hinged mode, one end, fixed to the movable plate 3, of the linkage rod 16 is located on the lower side of the middle end of the movable plate 3, and the rotating distance of the linkage rod 16 on the rotating disc 15 is smaller than the height of the placing cylinder 32 exceeding the upper surface of the end cover 2.
The rotating disc 15 is arranged to deviate from the center of the bottom plate 13, wherein the linkage rod 16 is arranged to deviate from the circular shape of the rotating disc 15, and the rotating disc 15 drives the movable plate 3 to move up and down through the linkage rod 16 by the rotation of the first motor shaft 14.
The non-slip mat 33 is made of rubber, wherein the thickness of the non-slip mat 33 is kept between three and five millimeters, the non-slip mat 33 is made of plastic with the same length as the depth of the groove of the placing cylinder 32.
The working principle is as follows: the household utensils with exosomes are inserted into the placing cylinder 32, the positions of the household utensils are fixed by friction through the anti-slip pad 33, then the first motor shaft 14 and the second motor shaft 23 can rotate, the rotating disc 15 drives the linkage rod 16 to rotate under the action of the first motor shaft 14, at the moment, under the pulling action of the linkage rod 16, the movable plate 3 reciprocates up and down, at the moment, the rectangular sliding block 12 slides in the inner groove 11, further, under the action of the fixed cylinder 31, the placing cylinder 32 can slide up and down in the first accommodating groove 21, further, under the rotation of the second motor shaft 23, the distance-increasing cylinder 2301 rotates with the semi-cylinder 2302, then, the semi-cylinder 2302 is in contact with the semi-cylinder 203, the semi-cylinder 203 can push the pushing rod 202, at the moment, the reset spring 204 deforms with accumulated force, and the further pushing rod 202 can push the placing cylinder 32 to be in contact with the arc-shaped plate 205, the inside household utensils of placing drum 32 at this moment can vibrate when carrying out different positions to exosomes, simultaneously under the promotion of arc 205, place drum 32 and rigging plate 27 and contact with elastic plate 26, elastic plate 26 can take place to hold power deformation this moment, and when half cylinder 2302 and hemisphere are full 203 and separate, elastic plate 26 makes and places drum 32 normal position, in addition under reset spring 204's effect, catch bar 202 normal position, and wait for next half cylinder 2302 and hemisphere cap 203 to contact, if reciprocating work, can reach the effect of vibrating exosomes, after work is accomplished, it can to stop the rotation of first motor shaft 14 and second motor shaft 23.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The utility model provides an oscillating device of exosome extraction usefulness, includes device shell (1), end cover (2) and fly leaf (3), wherein end cover (2) are fixed at the inner upside of shell (1), fly leaf (3) are located the inside of device shell (1), its characterized in that: the device is characterized in that built-in grooves (11) are symmetrically formed in the two sides of the inner end of a shell (1) of the device in a penetrating mode, rectangular sliding blocks (12) are placed inside the built-in grooves (11), a base plate (13) connected through screws is arranged on the lower side of the inner end of the shell (1), a first motor shaft (14) connected through a rotating shaft is arranged on one side of the base plate (13), a fixed rotating disc (15) is arranged at the upper end of the first motor shaft (14), a linkage rod (16) connected through a hinge mode is arranged on one side of the upper end of the rotating disc (15), a first accommodating groove (21) is formed in the middle end of an end cover (2) in a penetrating mode, a circular cylinder (22) connected through a welding mode is arranged on the upper side of the middle end of the end cover (2), a second motor shaft (23) connected through a rotating shaft is arranged at the middle end of the circular cylinder (22), a motor (24) connected through a fixed mode is arranged at the upper end of the second motor shaft (23), and second accommodating grooves (25) are uniformly formed in a penetrating mode on the side of the end cover (2), wherein elastic plate (26) are placed to second holding tank (25) inner one side, carry out screw connection between elastic plate (26) and end cover (2), wherein elastic plate (26) one end sets up laminating board (27) of screw connection, fly leaf (3) upper end one side evenly sets up fixed cylinder (31) of articulated connection, wherein fixed cylinder (31) inside place glue connection place cylinder (32), place cylinder (32) inner place glue connection's slipmat (33), wherein the one end of placing cylinder (32) runs through the inside setting of second holding tank (25).
2. An oscillating device for exosome extraction according to claim 1, characterized by: the inner end of the end cover (2) penetrates through the first accommodating groove (21) and is provided with a limiting clamping groove (201), wherein the inner end of the limiting clamping groove (201) penetrates through a push rod (202), one end of the push rod (202) is provided with a hemispherical cap (203) which is connected in a welding mode, a reset spring (204) is sleeved on the push rod (202), and one end of the push rod (202) is provided with an arc-shaped plate (205) which is connected in a hinged mode.
3. An oscillating device for exosome extraction according to claim 2, characterised in that: the hemispherical cap (203) is located inside the first accommodating groove (21), the radius of the hemispherical cap (203) is large and the maximum width of the return spring (204), one end of the return spring (204) is located inside the limiting clamping groove (201), the arc-shaped plate (205) is located inside the second accommodating groove (25), and the arc-shaped plate (205) is attached to the cylinder (32).
4. An oscillating device for exosome extraction according to claim 1, characterized by: circular cylinder (22) are located first holding tank (21) and are just up end, and wherein the groove diameter of first holding tank (21) is greater than the external diameter of placing drum (32), drum (32) setting is placed in the laminating of the one end of rigging board (27), and wherein the maximum length of rigging board (27) is the same with the internal diameter of first holding tank (21), the shape that elastic plate (26) set up is semicircle nature shape, and wherein the material that elastic plate (26) set up the adoption is the spring steel.
5. An oscillating device for exosome extraction according to claim 1, characterized by: the lower end of the second motor shaft (23) is provided with a distance-increasing cylinder (2301) connected by a screw, wherein the distance-increasing cylinder (2301) is positioned inside the first accommodating groove (21), the outer side of the distance-increasing cylinder (2301) is uniformly provided with half cylinders (2302), the number of the half cylinders (2302) is four, the angle interval between each half cylinder (2302) is ninety degrees, and the length of the distance-increasing cylinder (2301) is the same as the height of the end cover (2).
6. An oscillating device for exosome extraction according to claim 1, characterized by: the movable plate (3) is connected with the rectangular sliding block (12) in a welding mode, the movable plate (3) is connected with the linkage rod (16) in a hinged mode, one end, fixed to the movable plate (3), of the linkage rod (16) is located on the lower side of the middle end of the movable plate (3), and the rotating distance of the linkage rod (16) on the rotating disc (15) is smaller than the height, exceeding the upper surface of the end cover (2), of the cylinder (32).
7. An oscillating device for exosome extraction according to claim 1, characterized by: the non-slip mat (33) is made of rubber, the thickness of the non-slip mat (33) is kept between three and five millimeters, the length of the non-slip mat (33) is the same as the depth of the groove of the placing cylinder (32), and the material of the placing cylinder (32) is plastic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110532774.9A CN113150947A (en) | 2021-05-17 | 2021-05-17 | Vibration device for exosome extraction |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110532774.9A CN113150947A (en) | 2021-05-17 | 2021-05-17 | Vibration device for exosome extraction |
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| CN113150947A true CN113150947A (en) | 2021-07-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202110532774.9A Pending CN113150947A (en) | 2021-05-17 | 2021-05-17 | Vibration device for exosome extraction |
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| CN (1) | CN113150947A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113621489A (en) * | 2021-08-10 | 2021-11-09 | 安徽惠恩生物科技股份有限公司 | Culture method of Chongzhi stem cell factor essence and oscillation device thereof |
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| US20160230235A1 (en) * | 2013-09-25 | 2016-08-11 | The University Of Tokyo | Fluidic device, exosome analysis method, biomolecule analysis method, and biomolecule detection method |
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Application publication date: 20210723 |
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