CN113046236B - Culture bag operation equipment and operation system - Google Patents
Culture bag operation equipment and operation system Download PDFInfo
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- CN113046236B CN113046236B CN202110273817.6A CN202110273817A CN113046236B CN 113046236 B CN113046236 B CN 113046236B CN 202110273817 A CN202110273817 A CN 202110273817A CN 113046236 B CN113046236 B CN 113046236B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones
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Abstract
The invention discloses culture bag operation equipment and an operation system, wherein the culture bag operation equipment comprises a base, a manipulator is arranged at the center of the top end of the base, a turntable assembly and a pipetting module are respectively arranged on two sides of the manipulator along the length direction of the base, a shaking table and a pipetting module are respectively arranged at the centers of the two ends of the manipulator along the width direction of the base, a cover opener is arranged on one side, close to the turntable assembly, of the pipetting module, a centrifuge module is arranged at the corner, close to the base, of the top end of the base, between the pipetting module and the pipetting module, and the automation operation is applicable to batch automatic production; the full-automatic operation is carried out, so that the contact with operators is avoided, and the pollution risk is reduced; the full-automatic operation is realized, and the whole system can be placed in a closed clean environment, so that aseptic operation is facilitated; realizing the whole process integrated operation of the culture bag, such as liquid supplementation, waste liquid replacement, sampling detection and centrifugal harvesting.
Description
Technical Field
The invention belongs to the technical field of medicine production, and particularly relates to culture bag operation equipment and an operation system.
Background
In the rapid development period of biological medicines, in the process of biological pharmacy, some processes can use culture bags to culture cells, and the traditional culture bags are generally manually operated, so that the efficiency is low, operators are required to participate, and the pollution risk exists. Therefore, a culture bag operating system is needed, full-automatic operation is realized, personnel participation is reduced or even eliminated, working efficiency is improved, and pollution risk is reduced.
Disclosure of Invention
The invention aims to provide a culture bag operation device and an operation system, which are used for solving the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the utility model provides a cultivate bag operation equipment, includes the base, the center on base top goes out to be equipped with the manipulator, the base is equipped with carousel subassembly and pipetting module respectively along length direction and be located the both sides of manipulator, the base is equipped with shaking table and pump liquid module respectively along width direction and be located the both ends of manipulator and open into the center, one side that the pump liquid module is close to carousel subassembly is equipped with the uncapping machine, the base top is located between pipetting module and the pump liquid module and is close to base corner and is equipped with centrifuge module.
Further, the carousel subassembly includes carousel, joins in marriage liquid and waste container, material frame, the carousel is established on the base top, the material is erect on the top of carousel, join in marriage liquid and waste container and establish in the carousel one side of keeping away from the pipetting module.
Further, the pump liquid module comprises a liquid medicine cabinet, a pump body, a liquid distribution bottle, a waste liquid bottle and a pump liquid needle, wherein the liquid medicine cabinet is arranged below the base, and the pump body, the liquid distribution bottle, the waste liquid bottle and the pump liquid needle are connected with the base through a supporting plate.
Further, the centrifugal machine module comprises a centrifugal machine, a centrifugal machine sealing sleeve and a centrifugal machine cover, wherein the centrifugal machine is arranged at the bottom of the base, the centrifugal machine sealing sleeve is arranged between the centrifugal machine and the base, and the centrifugal machine cover is arranged at the top end of the base and is positioned right above the centrifugal machine.
Further, a concave support with a downward opening is arranged on one side, far away from the manipulator, of the pipetting module, a motor is arranged in the support, and the output end of the motor penetrates through the side wall of the support to be connected with the pipetting module.
Further, the pipetting module comprises a pipetting bottle or a centrifugal bottle, a syringe pulling and pushing mechanism, a syringe, a culture bag clamp, a liquid extruding mechanism and a culture bag assembly, wherein the syringe pulling and pushing mechanism is fixed at the top end of the pipetting module, which is close to one side of the manipulator, the syringe is clamped on the syringe pulling and pushing mechanism, the culture bag assembly is arranged below the syringe, the liquid extruding mechanism is arranged at the center of the culture bag assembly, which is far away from one side of the manipulator, and the culture bag clamp and the pipetting bottle or the centrifugal bottle are both positioned at the top end of the culture bag assembly to be matched with the syringe.
A culture bag handling system characterized by the following steps:
s1: the feeding is placed on the turntable assembly;
s2: the mechanical arm clamps the liquid preparation bottle, conveys the liquid preparation bottle to a cover opening machine for opening a cover, and conveys the liquid preparation bottle to a liquid pumping module for receiving liquid medicine;
s3: the manipulator clamps the culture bag assembly and conveys the culture bag assembly to the pipetting module.
S4: the mechanical arm clamps a liquid preparation bottle filled with liquid medicine and conveys the liquid preparation bottle to the liquid transferring module;
s5: transferring the liquid medicine from the liquid preparation bottle to a culture bag by using a syringe;
s6: placing the culture bag into a shaking table for shaking uniformly;
s7: the manipulator clamps the culture bag from the cradle and puts the culture bag into the turntable assembly for the next operation of the system;
s8: if centrifugation is needed, liquid is extracted from the culture bag by a syringe and is moved to a centrifugal bottle, then the centrifugal bottle is clamped by a manipulator, a centrifugal cover is opened, and the centrifugal bottle is placed in a centrifugal machine.
Compared with the prior art, the invention has the beneficial effects that:
1. the full-automatic operation is suitable for batch automatic production;
2. the full-automatic operation is carried out, so that the contact with operators is avoided, and the pollution risk is reduced;
3. the full-automatic operation is realized, and the whole system can be placed in a closed clean environment, so that aseptic operation is facilitated;
4. realizing the whole process integrated operation of the culture bag, such as liquid supplementation, waste liquid replacement, sampling detection and centrifugal harvesting.
Drawings
FIG. 1 is a perspective view of a bag handling apparatus and handling system according to the present invention;
FIG. 2 is a perspective view of a bag handling apparatus and handling system according to the present invention.
In the figure: 1. a turntable assembly; 11. a turntable; 12. liquid preparation and waste container; 13. a material rack; 2. a cover opening machine; 3. a liquid pumping module; 31. a liquid medicine cabinet; 32. a pump body; 33. a liquid preparation bottle and a waste liquid bottle; 34. a liquid pumping needle; 4. a manipulator; 5. a centrifuge module; 51. a centrifuge; 52. sealing sleeve of centrifugal machine; 53. a centrifuge cover; 6. a pipetting module; 61. pipetting or centrifugation bottles; 62. a syringe pulling mechanism; 63. a syringe; 64. a culture bag clamp; 65. a liquid extrusion mechanism; 66. a culture bag assembly; 7. shaking table; 8. a base; 9. and a motor.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments, and all other embodiments obtained by those skilled in the art without making creative efforts based on the embodiments of the present invention are included in the protection scope of the present invention.
In order that the invention may be readily understood, a more complete description of the invention will be rendered by reference to the appended drawings. Several embodiments of the invention are presented in the figures. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "mounted" on another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items
The invention provides a technical scheme that:
embodiment 1, please refer to fig. 1 and 2, a culture bag operation equipment, which comprises a base 8, the center of base 8 top goes out to be equipped with manipulator 4, base 8 is equipped with carousel subassembly 1 subassembly and pipetting module 6 respectively along length direction and be located the both sides of manipulator 4, base 8 is equipped with shaking table 7 and pump liquid module 3 respectively along width direction and be located the both ends center of opening of manipulator 4, one side that pump liquid module 3 is close to carousel subassembly 1 subassembly is equipped with lid machine 2, base 8 top is located between pipetting module 6 and pump liquid module 3 and is close to base 8 corner and is equipped with centrifuge module 5, and its position of introducing every module and subassembly.
In example 2, referring to fig. 1 and 2, the turntable assembly 1 comprises a turntable 11, a liquid preparation and waste container 12 and a material rack 13, wherein the turntable 11 is disposed at the top end of a base 8, the material rack 13 is disposed at the top end of the turntable 11, the liquid preparation and waste container 12 is disposed at one side of the turntable 11 away from a pipetting module 6, the liquid pumping module 3 comprises a liquid medicine tank 31, a pump body 32, a liquid preparation bottle and waste liquid bottle 33 and a liquid pumping needle 34, the liquid medicine tank 31 is disposed below the base 8, the pump body 32, the liquid preparation bottle and waste liquid bottle 33 and the liquid pumping needle 34 are connected with the base 8 through a support plate, the centrifuge module 5 comprises a centrifuge 51, a centrifuge sealing sleeve 52 and a centrifuge cover 53, the centrifuge 51 is disposed at the bottom of the base 8, the centrifuge sealing sleeve 52 is disposed between the centrifuge 51 and the base 8, the centrifuge cover 53 is disposed at the top end of the base 8 and is located right above the centrifuge 51, a concave bracket with a downward opening is arranged on one side of the pipetting module 6 far away from the manipulator 4, a motor 9 is arranged in the bracket, the output end of the motor 9 penetrates through the side wall of the bracket and is connected with the pipetting module 6, the pipetting module 6 comprises a pipetting bottle or centrifugal bottle 61, a syringe pulling and pushing mechanism 62, a syringe 63, a culture bag clamp 64, a liquid extrusion mechanism 65 and a culture bag assembly 66, the syringe pulling and pushing mechanism 62 is fixed on the top end of the pipetting module 6 near one side of the manipulator 4, the syringe 63 is clamped on the syringe pulling and pushing mechanism 62, the culture bag assembly 66 is arranged below the syringe 63, the liquid extrusion mechanism 65 is arranged at the center of the culture bag assembly 66 far away from one side of the manipulator 4, the culture bag clamp 64 and the pipetting bottle or centrifugal bottle 61 are both positioned on the top end of the culture bag assembly 66 and are matched with the syringe 63, the specific component locations of each assembly and module are described in detail above.
Example 3 referring to fig. 1 and 2, a culture bag handling system is provided, which comprises the following steps:
s1: the feeding is placed on the turntable assembly 1;
s2: the mechanical arm 4 clamps the liquid preparation bottle, conveys the liquid preparation bottle to the cover opener 2 for uncovering, and conveys the liquid preparation bottle to the liquid pumping module 3 for receiving liquid medicine;
s3: the manipulator 4 grips the culture bag assembly 66 for transport to the pipetting module 6.
S4: the mechanical arm 4 clamps a liquid preparation bottle filled with liquid medicine and conveys the liquid preparation bottle to the pipetting module 6;
s5: transferring the liquid medicine from the liquid preparation bottle to the culture bag by using a syringe 63;
s6: putting the culture bag into a shaking table 7 for shaking uniformly;
s7: the manipulator 4 clamps the culture bags from the shaking table 7 and puts the bags into the turntable assembly 1 for the next operation of the system;
s8: if centrifugation is required, the liquid is withdrawn from the culture bag by the syringe 63 and transferred to the centrifuge bottle, and then the centrifuge bottle is gripped by the manipulator 4, the centrifuge cover 53 is opened, and the centrifuge bottle is placed in the centrifuge 51.
Embodiment 4, the corresponding components, assemblies and modules function as follows:
turntable assembly 1: the materials required by the system to run are delivered and placed on the turntable assembly 1, meanwhile, the turntable assembly 1 is also used for storing the materials, and the turntable assembly 1 comprises a turntable 11, a liquid preparation and waste container 12, a material rack 13 and a syringe material rack 14.
Cover opener 2: the lid is opened and closed for the dispensing and waste container 12.
Pump liquid module 3: the pump liquid module 3 is used for preparing liquid medicine required by the system, wherein various liquid medicines required by the system, disinfectant and cleaning liquid are stored in the liquid medicine cabinet 31. The various liquid medicines are pumped into a liquid preparation bottle 33 through a pump 32 and a liquid preparation needle 34, and the required components are prepared according to a certain proportion. After the disinfection and cleaning fluid disinfects and cleans the pipeline, the disinfection fluid and cleaning fluid are pumped into the waste liquid bottle 33.
Manipulator 4: for transporting materials between stations.
Centrifuge module 5: is used for separating effective components. The centrifugal machine comprises a centrifugal machine 51, a centrifugal machine sealing sleeve 52 and a centrifugal machine cover 53, wherein the centrifugal machine cover 53 is driven by a motor and is controlled automatically.
Pipetting module 6: the liquid transferring device is used for transferring liquid, comprising the steps of transferring the liquid medicine prepared by the liquid pumping module into a culture bag, taking out cultured cells from the culture bag and conveying the cells to the next station. The liquid medicine prepared in the liquid preparation bottle 33 of the liquid pumping module 3 is transported to the liquid preparation bottle 61, the liquid transferring module 6 operates to enable the needle of the syringe 63 to be inserted into the liquid preparation bottle 61, then the syringe drawing and pushing mechanism 62 moves to suck liquid from the liquid preparation bottle 61, then the liquid transferring module 6 operates to enable the needle of the syringe 63 to be inserted into the culture bag of the culture bag assembly 66, then the syringe drawing and pushing mechanism 62 moves to enable the liquid in the syringe 63 to be injected into the liquid preparation bag. If it is desired to transfer liquid from the bag to elsewhere, the operation is reversed from that described above. In the pipetting process, the culture bag assembly is fixed and positioned in the pipetting module 6 by the culture bag clamp 64, and when liquid is taken from the culture bag, the liquid extrusion mechanism 65 compresses the culture bag, so that the culture bag is always uniformly filled with liquid, and the difficulty in taking liquid due to the fact that the liquid is deposited on the bottom plate of the culture bag under the action of gravity is avoided.
Cradle 7: the shaking table 7 is used for evenly mixing the liquid in the culture bag and the liquid preparation bottle.
Base 8: the base 8 is the installation foundation of the whole system.
Rotating electric machine 9: the motor 9 can drive the pipetting module 6 to rotate a certain angle, so that if air exists in the syringe 63 or the culture bag 66 during pipetting, the air can be moved to the bottom or the top of the syringe 66 or the culture bag 66, and the influence of the sucked air on pipetting accuracy is avoided.
Example 5, referring to figures 1 and 2,
the standard operational flow-sampling procedure is as follows:
firstly, various materials (culture bags, disposable syringes and sampling tubes) are placed on a rotary material rack 1 in order;
then the culture bags are placed on a shaking table 7 for mechanical arm transportation, and shaking of the culture bags is carried out uniformly;
then the mechanical arm carries the disposable syringe to the syringe pipetting module 6;
the mechanical arm transfers the sampling tube to the cover opener 2 to open the cover of the sampling tube;
the manipulator transfers the uncapped sampling tube to the injector pipetting module 6;
transferring the evenly mixed culture bags to a syringe pipetting module 6 by a manipulator;
the disposable injector performs puncture sampling on the culture bag;
the disposable syringe transfers the sampling liquid into the sampling tube;
the mechanical arm transfers the sampling tube to the cover opener 2, closes the cover of the sampling tube, and transfers the sampling tube to the detection procedure;
the mechanical arm transfers the disposable injector which is used up as waste material out;
finally, transferring the culture bag out of the operation unit.
The standard operation flow-liquid adding steps are as follows:
firstly, various materials (culture bags, disposable syringes and liquid mixing bottles) are placed on a rotary material rack 1 in order;
the manipulator then transfers the disposable syringe to the syringe pipetting module 6;
then the mechanical arm transfers the mixed liquid bottle to the cover opener 2 for opening the cover of the mixed liquid bottle;
transferring the liquid mixing bottle to a liquid pumping module 3 by a mechanical arm for liquid filling;
the mechanical arm is used for filling the liquid and then transferring the liquid-mixing bottle to the injector pipetting module 6;
the disposable syringe absorbs liquid from the liquid mixing bottle;
the disposable injector performs puncture sampling on the culture bag;
a disposable syringe fills the culture bag with liquid;
the mechanical arm transfers the mixed liquid bottle to the cover opener 2 for cover closing operation and to a garbage position;
the mechanical arm transfers the disposable injector which is used up as waste material out;
finally, transferring the culture bag out of the operation unit;
standard procedure-centrifugation enrichment:
firstly, various materials (culture bags, disposable syringes and centrifugal bottles) are placed on a rotary material rack 1 in order;
then the mechanical arm carries the disposable syringe to the syringe pipetting module 6;
transferring the culture bag to a shaking table 7 by a mechanical arm, and uniformly mixing a culture system;
transferring the centrifugal bottle to a cover opener by a mechanical arm to open the cover;
the manipulator conveys the centrifuge bottle after uncovering to the injector pipetting module 6;
the culture bags after being evenly mixed are transported to the injector pipetting module 6 by the mechanical arm;
the disposable injector punctures the culture bag and sucks out the liquid in the culture bag;
the disposable syringe transfers the liquid into a centrifuge bottle;
the mechanical arm transfers the centrifugal bottle to the cover opener 2 to perform cover closing operation;
transferring the centrifugal bottle into a centrifugal machine by a mechanical arm for centrifugal operation;
after the centrifugal operation is completed, the manipulator transfers the centrifugal bottle to the cover opener 2 for uncovering;
the manipulator transfers the centrifuge bottle to the syringe pipetting module 6 again;
the disposable injector sucks the upper waste supernatant of the centrifugal bottle and discharges the supernatant to a waste liquid bag (original culture bag);
after the centrifugal operation is finished, the manipulator transfers the centrifugal bottle to the cover opener 2, closes the cover and waits for the next operation;
the mechanical arm transfers the disposable injector which is used up as waste material out;
finally, transferring the waste liquid (culture) bag out of the operation unit.
In conclusion, the full-automatic operation is suitable for batch automatic production; the full-automatic operation is carried out, so that the contact with operators is avoided, and the pollution risk is reduced; the full-automatic operation is realized, and the whole system can be placed in a closed clean environment, so that aseptic operation is facilitated; realizing the whole process integrated operation of the culture bag, such as liquid supplementation, waste liquid replacement, sampling detection and centrifugal harvesting.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (3)
1. Culture bag handling apparatus comprising a base (8), characterized in that: the center of base (8) top goes out to be equipped with manipulator (4), base (8) are equipped with carousel subassembly (1) and pipetting module (6) respectively along length direction and are located the both sides of manipulator (4), base (8) are equipped with shaking table (7) and pump liquid module (3) respectively along width direction and are located the both ends center department of opening of manipulator (4), one side that pump liquid module (3) are close to carousel subassembly (1) is equipped with lid machine (2), base (8) top is located between pipetting module (6) and pump liquid module (3) and is close to base (8) corner department and is equipped with centrifuge module (5);
the turntable assembly (1) comprises a turntable (11), a liquid preparation and waste container (12) and a material rack (13), wherein the turntable (11) is arranged at the top end of the base (8), the material rack (13) is arranged at the top end of the turntable (11), and the liquid preparation and waste container (12) is arranged at one side of the turntable (11) far away from the liquid transfer module (6);
the liquid pumping module (3) comprises a liquid medicine cabinet (31), a pump body (32), a liquid preparation bottle, a waste liquid bottle (33) and a liquid pumping needle (34), wherein the liquid medicine cabinet (31) is arranged below the base (8), and the pump body (32), the liquid preparation bottle, the waste liquid bottle (33) and the liquid pumping needle (34) are connected with the base (8) through a supporting plate;
the centrifugal machine module (5) comprises a centrifugal machine (51), a centrifugal machine sealing sleeve (52) and a centrifugal machine cover (53), wherein the centrifugal machine (51) is arranged at the bottom of the base (8), the centrifugal machine sealing sleeve (52) is arranged between the centrifugal machine (51) and the base (8), and the centrifugal machine cover (53) is arranged at the top end of the base (8) and is positioned right above the centrifugal machine (51);
the liquid transferring module (6) comprises a liquid transferring bottle or a centrifugal bottle (61), a syringe pulling and pushing mechanism (62), a syringe (63), a culture bag clamp (64), a liquid extruding mechanism (65) and a culture bag assembly (66), wherein the syringe pulling and pushing mechanism (62) is fixed at the top end of one side, close to the manipulator (4), of the liquid transferring module (6), the syringe (63) is clamped on the syringe pulling and pushing mechanism (62), the culture bag assembly (66) is arranged below the syringe (63), the liquid extruding mechanism (65) is arranged at the center of one side, far away from the manipulator (4), of the culture bag assembly (66), and the top end of the culture bag clamp (64) and the syringe (61) are both located on the culture bag assembly (66) to be matched with the syringe (63).
2. The culture bag handling apparatus of claim 1, wherein: a concave support with a downward opening is arranged on one side, far away from the manipulator (4), of the pipetting module (6), a motor (9) is arranged in the support, and the output end of the motor (9) penetrates through the side wall of the support and is connected with the pipetting module (6).
3. A culture bag handling system, characterized in that it is realized by using a culture bag handling apparatus according to any one of claims 1-2, comprising the following steps:
s1: the feeding is arranged on the turntable assembly (1);
s2: the mechanical arm (4) clamps the liquid preparation bottle, conveys the liquid preparation bottle to the cover opening machine (2) for opening the cover, and conveys the liquid preparation bottle to the liquid pumping module (3) for receiving liquid medicine;
s3: the manipulator (4) clamps the culture bag assembly (66) and conveys the culture bag assembly to the pipetting module (6);
s4: the mechanical arm (4) clamps a liquid preparation bottle filled with liquid medicine and conveys the liquid preparation bottle to the liquid transferring module (6);
s5: transferring the liquid medicine from the liquid preparation bottle to the culture bag by using a syringe (63);
s6: putting the culture bag into a shaking table (7) for shaking uniformly;
s7: the manipulator (4) clamps the culture bags from the shaking table (7) and puts the culture bags into the turntable assembly (1) for the next operation of the system;
s8: if centrifugation is required, liquid is extracted from the culture bag by a syringe (63) and moved to a centrifuge bottle, then the centrifuge bottle is clamped by a manipulator (4), a centrifuge cover (53) is opened, and the centrifuge bottle is placed in a centrifuge (51).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110273817.6A CN113046236B (en) | 2021-03-15 | 2021-03-15 | Culture bag operation equipment and operation system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110273817.6A CN113046236B (en) | 2021-03-15 | 2021-03-15 | Culture bag operation equipment and operation system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113046236A CN113046236A (en) | 2021-06-29 |
| CN113046236B true CN113046236B (en) | 2023-10-20 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110273817.6A Active CN113046236B (en) | 2021-03-15 | 2021-03-15 | Culture bag operation equipment and operation system |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105022411A (en) * | 2015-07-06 | 2015-11-04 | 中国人民解放军第三军医大学第二附属医院 | Clinic automatic dispensing system |
| CN210974703U (en) * | 2019-09-29 | 2020-07-10 | 深圳赛动生物自动化有限公司 | Automatic cell batch production equipment |
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| US20160354281A1 (en) * | 2009-03-03 | 2016-12-08 | Bryan M. O'NEILL | Automated transfer of liquid medicament from containers |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105022411A (en) * | 2015-07-06 | 2015-11-04 | 中国人民解放军第三军医大学第二附属医院 | Clinic automatic dispensing system |
| CN210974703U (en) * | 2019-09-29 | 2020-07-10 | 深圳赛动生物自动化有限公司 | Automatic cell batch production equipment |
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