CN112964638A - Immunoassay integrated system - Google Patents
Immunoassay integrated system Download PDFInfo
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- CN112964638A CN112964638A CN202110150265.XA CN202110150265A CN112964638A CN 112964638 A CN112964638 A CN 112964638A CN 202110150265 A CN202110150265 A CN 202110150265A CN 112964638 A CN112964638 A CN 112964638A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses an immunoassay comprehensive system, which has the following beneficial effects: 1. based on the support body setting that has first inner chamber, the integration has hatching mechanism, fluorescence detection mechanism and process transfer mechanism, carries out fluorescence detection again after having carried out the processing of hatching, transfers by process transfer mechanism at this transfer process in-process, consequently avoids carrying out remote transfer to the test sample, improves efficiency of software testing, test stability. 2. Based on the fact that the incubation grooves are arranged in a straight line in the vertical direction, and the positions of the test ports correspond to the positions of the incubation grooves up and down, the efficiency and the stability of the transfer process are improved through a smaller process transfer distance in the process of transferring the processes; and the miniature design of the incubation mechanism is facilitated, and excessive space is not occupied. 3. Is suitable for incubation of different test items.
Description
Technical Field
The invention belongs to the technical field of testing, and particularly relates to an immunoassay comprehensive system.
Background
At present, incubation and various testing treatments are required to be carried out on a test sample, and a testing mechanism and an incubation mechanism are independent, so that the problem of low testing efficiency of the test sample is caused due to the fact that procedure transfer is required. And there is currently no such integrated system that combines a testing mechanism and an incubation mechanism.
Therefore, the prior art is to be improved.
Disclosure of Invention
The invention mainly aims to provide an immunoassay comprehensive system.
The invention relates to an immunoassay comprehensive system which comprises a bracket body with a first inner cavity, an incubation mechanism arranged in the first inner cavity, a fluorescence detection mechanism arranged at the top of the bracket body, a two-dimensional code scanning module used for scanning a two-dimensional code on a test card, and a procedure transfer mechanism used for transferring procedures of the test card, wherein a test port used for inserting the test card is arranged on the fluorescence detection mechanism, a plurality of incubation grooves used for placing the test card are arranged on the front end surface of the incubation mechanism, and the incubation grooves are linearly arranged in the vertical direction.
Preferably, the device further comprises a substrate, and the bracket body and the process transfer mechanism are arranged on the substrate.
Preferably, the process transfer mechanism comprises a lifting platform, a screw rod connected with the lifting platform, a horizontal guide rail arranged on the lifting platform, a card shifting electromagnetic valve, a horizontal card shifting motor connected with the card shifting electromagnetic valve and a vertical lifting motor connected with the screw rod, wherein the card shifting electromagnetic valve is used for pushing the test card into the incubation groove or the test port from the horizontal guide rail.
Preferably, a sliding groove is formed in the horizontal guide rail, an in-car test card in-place detection mechanism is arranged at one end, close to the incubation groove, of the sliding groove, and a card dialing in-place detection mechanism is arranged at one end, close to the two-dimensional code scanning module, of the sliding groove.
Preferably, the test card reader further comprises an insertion mechanism, an insertion position used for inserting the test card is arranged on the insertion mechanism, and the two-dimensional code scanning module is located above the insertion mechanism.
Preferably, the test card detection mechanism is arranged on the insertion mechanism.
Preferably, the incubation mechanism comprises a main shell provided with a second inner cavity, a radiator used for radiating heat of the second inner cavity, a cooling system used for refrigerating the second inner cavity, and a temperature control plate, wherein the temperature control plate is connected with the cooling system, a plurality of incubation grooves are formed in the front end face of the main shell, and the incubation grooves are communicated with the second inner cavity.
Preferably, the number of heat sinks is at least two.
Preferably, a heating module electrically connected with the temperature control plate is arranged in the second inner cavity.
Preferably, the bottom of the main housing is provided with a condensed water drain outlet.
The immunoassay comprehensive system has the following beneficial effects:
1. based on the support body setting that has first inner chamber, the integration has hatching mechanism, fluorescence detection mechanism and process transfer mechanism, carries out fluorescence detection again after having carried out the processing of hatching, transfers by process transfer mechanism at this transfer process in-process, consequently avoids carrying out remote transfer to the test sample, improves efficiency of software testing, test stability.
2. Based on the fact that the incubation grooves are arranged in a straight line in the vertical direction, and the positions of the test ports correspond to the positions of the incubation grooves up and down, the efficiency and the stability of the transfer process are improved through a smaller process transfer distance in the process of transferring the processes; and the miniature design of the incubation mechanism is facilitated, and excessive space is not occupied.
3. The two-dimensional code scanning module identifies the two-dimensional code on the test card, determines the corresponding test item according to the two-dimensional code, and inserts the test card into a plurality of incubation grooves corresponding to the test item through the process transfer mechanism so as to perform incubation in corresponding time, so that the test card is suitable for incubation of different test items.
Drawings
FIG. 1 is a first three-dimensional schematic diagram of the internal structure of an immunoassay integration system of the present invention;
FIG. 2 is a schematic structural diagram of an incubation mechanism and a fluorescence detection mechanism according to the present invention;
FIG. 3 is a second three-dimensional schematic view of the internal structure of the immunoassay integration system of the present invention;
FIG. 4 is a three-dimensional schematic view of the inner structure of the immunoassay integrated system of the present invention after the upper protective shell is assembled.
The implementation, functional features and advantages of the objects of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. It is noted that relative terms such as "first," "second," and the like may be used to describe various components, but these terms are not intended to limit the components. These terms are only used to distinguish one component from another component. For example, a first element could be termed a second element, and, similarly, a second element could be termed a first element, without departing from the scope of the present invention. The term "and/or" refers to a combination of any one or more of the associated items and the descriptive items.
As shown in fig. 1 and 2, fig. 1 is a first three-dimensional schematic diagram of the internal structure of the immunoassay comprehensive system of the present invention; FIG. 2 is a schematic structural diagram of an incubation mechanism and a fluorescence detection mechanism according to the present invention; the invention relates to an immunoassay comprehensive system, which comprises a bracket body 10 with a first inner cavity 100, an incubation mechanism 200 arranged in the first inner cavity 100, a fluorescence detection mechanism 20 arranged at the top of the bracket body 10, a two-dimensional code scanning module 400 used for scanning a two-dimensional code on a test card 30, and a procedure transfer mechanism used for carrying out procedure transfer on the test card, wherein the fluorescence detection mechanism is provided with a test port 21 used for inserting the test card, the front end surface of the incubation mechanism 200 is provided with a plurality of incubation grooves 41 used for placing the test card, and the incubation grooves 41 are linearly arranged in the vertical direction; wherein, the vertical direction refers to the direction perpendicular to the substrate 666; the procedures comprise a detection procedure of the insertion mechanism, a detection procedure of the test card in the sliding groove, an incubation procedure of the incubation mechanism and a detection procedure of the fluorescence detection mechanism. The immunoassay comprehensive system has the following beneficial effects: 1. based on the support body setting that has first inner chamber, the integration has hatching mechanism, fluorescence detection mechanism and process transfer mechanism, carries out fluorescence detection again after having carried out the processing of hatching, transfers by process transfer mechanism at this transfer process in-process, consequently avoids carrying out remote transfer to the test sample, improves efficiency of software testing, test stability. 2. Based on the fact that the incubation grooves are arranged in a straight line in the vertical direction, and the positions of the test ports correspond to the positions of the incubation grooves up and down, the efficiency and the stability of the transfer process are improved through a smaller process transfer distance in the process of transferring the processes; and the miniature design of the incubation mechanism is facilitated, and excessive space is not occupied. 3. The two-dimensional code scanning module identifies the two-dimensional code on the test card, determines the corresponding test item according to the two-dimensional code, and inserts the test card into a plurality of incubation grooves corresponding to the test item through the process transfer mechanism so as to perform incubation in corresponding time, so that the test card is suitable for incubation of different test items.
The following will specifically describe the integrated design structure: the device also comprises a substrate 666, wherein the bracket body 10 and the process transfer mechanism are arranged on the substrate 666; the substrate 666 is provided to realize the integrated design of the bracket body, the process transfer mechanism, the incubation mechanism and the fluorescence detection mechanism.
And as shown in FIG. 2; the structural design of the bracket body is as follows: the support body 10 includes a first bottom plate 4, a first support column 1, a second support column 3, a third support column 2, a fourth support column (not shown and labeled in fig. 2) and an upper support plate 5, the four support columns realize a support function and can enable a receiving space formed between the first bottom plate 4 and the upper support plate 5, namely, a first inner cavity 100, due to the design of the four columns.
As shown in fig. 1, the process transferring mechanism includes a lifting platform 551, a horizontal guide 552 disposed on the lifting platform 551, a card-moving solenoid valve 109, a horizontal card-moving motor 106 connected to the card-moving solenoid valve 109, a screw 2223 connected to the lifting platform 551, and a vertical lifting motor 105 connected to the screw 2223, wherein the card-moving solenoid valve 109 is used to push the test card from the horizontal guide 552 into the incubation slot 41 or the test port 21. The preferred embodiment defines the specific structural composition and the specific structural connection of the process transfer mechanism; the short-distance transfer of the test card is realized between the incubation process and the fluorescence detection process, the card shifting solenoid valve 109 is provided with a shifting pin 2222, and the shifting pin 2222 can be inserted into a bayonet of the test card, so that the test card can be accurately driven to rapidly move in each process; so that the power provided by the horizontal card-shifting motor can drive the test card to move on the horizontal guide rail to enter the incubation groove 41 or the test port 21; since the position of the incubation groove 41 of the incubation mechanism is below the test port 21 of the fluorescence detection mechanism (the position of the test port corresponds to the position of the incubation groove up and down), the screw 2223 can be driven by the vertical lifting motor 105 to rotate, so that the lifting platform 551 can be lifted, that is, the incubation mechanism can complete the incubation treatment and then transfer the incubation treatment to the test port 21 through the incubation groove 41. Namely, when the screw rod rotates forwards, the lifting platform rises; when the screw rod rotates reversely, the lifting platform descends.
Wherein, vertical guide rails 550 are also included, and the number of the vertical guide rails can be four; the vertical guide rails are vertically disposed on the substrate 666; specifically, the vertical guide rail penetrates through the lifting platform, namely the lifting platform can carry out lifting operation on the vertical guide rail; the guiding function is achieved.
As shown in fig. 1, the two-dimensional code scanning device further comprises an insertion mechanism 502, wherein an insertion position 501 of an insertion position for inserting the test card and an output port 503 for outputting the test card are arranged on the insertion mechanism 502, and the two-dimensional code scanning module 400 is located above the insertion mechanism; after the test card 30 is inserted into the insertion position 501, the two-dimensional code scanning module 400 is configured to identify a two-dimensional code on the test card to determine test items of the test card, where different test items correspond to different incubation slots.
As shown in fig. 1, further includes an actuator motor 102 for displacing the test card 30 from the insertion position 501 to the output opening 503; a limit solenoid valve 103 is also included on the interposer 502 to provide a limit function.
As shown in fig. 1, preferably, a sliding groove 1001 is provided on the horizontal guide rail, an in-car test card in-place detection mechanism 108 is provided at one end of the sliding groove close to the incubation groove, and a card-dialing in-place detection mechanism 107 is provided at one end of the sliding groove close to the two-dimensional code scanning module. The in-place detection mechanism 108 for the test card in the car is used for detecting whether the test card enters the sliding groove 1001; the card-pushing-in-place detection mechanism 107 is used for detecting whether the pushing pin 2222 completely enters the position corresponding to the card opening of the test card; if the test card does not completely enter the sliding groove 1001, the in-place test card detection mechanism 108 in the car will feed back to the control system to prompt; otherwise, the test card is easily extruded and damaged due to the fact that the test card is not completely inserted into the sliding groove 1001 and the vertical lifting motor is driven. The device further comprises a test card detection mechanism 110 disposed on the insertion mechanism for detecting whether a test card is inserted into the insertion position of the insertion mechanism.
As shown in fig. 1, the device further includes an upper protection plate 667, one end of the vertical rail 550 is connected to the substrate 666, and the other end is connected to the upper protection plate 667; the vertical guide rails are straight bars, and the number of the vertical guide rails can be four.
As shown in fig. 1, further comprising an anti-collision switch 300 disposed on the incubation mechanism; the anti-collision effect is achieved.
The incubation mechanism comprises a main shell provided with a second inner cavity, a radiator 11 used for radiating heat of the second inner cavity, a cooling system used for refrigerating the second inner cavity and a temperature control plate 50 connected with the cooling system, wherein a plurality of incubation grooves 41 are arranged on the front end surface of the main shell and are communicated with the second inner cavity; and a heating module electrically connected with the temperature control plate is arranged in the second inner cavity. A heating module electrically connected with the temperature control plate and a cooling system electrically connected with the temperature control plate are arranged in the second inner cavity; the main housing may be a rectangular body shaped housing, and the second inner cavity represents an inner space of the housing.
Specifically, the method comprises the following steps: the heating module is used for heating the second inner cavity; the cooling system is used for refrigerating the second inner cavity; the incubation groove is used for inserting and accommodating the test card 30 so as to allow the test card to stand for a certain time, so that a test sample in the test card reaches a preset temperature; the temperature control plate is used for controlling the heating module and the cooling system.
As shown in fig. 2, the number of heat sinks is at least two; the radiator is a radiating fan.
Wherein, the bottom of the main shell is provided with a condensed water outlet 42; the inside cooling system that is used for carrying out the cooling to the second inner chamber that still is provided with of main casing body, and comdenstion water outlet 42 is cooling system's delivery outlet to carry out liquid discharge.
Wherein, the main shell is provided with a waste card outlet 60; for ejection of waste test cards.
Wherein the number of the incubation grooves is 2-10; the specific number can be adjusted according to actual conditions; the multiple incubation grooves are designed to realize incubation treatment of different test items; for example, the A test item needs to be incubated for 2 minutes; the test item B needs incubation for 10 minutes; because the test items are different, different incubation tanks can be selected to enter; of course, the test item determination is determined according to the two-dimensional code on the test card.
More specifically: the fluorescence detection mechanism is used for carrying out fluorescence detection on the test card 30; the fluorescence detection mechanism is arranged on the upper support plate 5; the upper supporting plate 5 is also provided with a main control plate 61 for controlling the fluorescence detection mechanism.
As shown in fig. 3, the display device further includes a power source 703 disposed on the substrate 666, and a power filter 702 connected to the power source 703; so as to supply power to each motor, the fluorescence detection mechanism, the two-dimensional code scanning module, the incubation mechanism and the like.
As shown in fig. 3, a vertical zero detection mechanism 704 is also included; the vertical zero position detection mechanism 704 is used for detecting the rotation number of the vertical lifting motor so as to send the rotation number to the control module; to determine the height of the lifting platform. For example, the number of rotations is 2, which indicates that the height of the lifting platform is flush with the incubation groove at the second height; the number of rotations is 4, indicating that the height of the lifting platform is flush with the incubation tank 41 at the fourth height; the number of turns is 6, indicating that the elevation platform is at a level flush with the test port 21 at the sixth elevation.
As shown in fig. 3, further comprises a fluorescence detection card-entering motor 700; so as to provide a driving force to drive the test card to enter the fluorescence detection mechanism from the test port 21 for testing.
As shown in fig. 3, the incubation device further comprises a heat-dissipating air duct 701 disposed at the back of the incubation mechanism; to provide some heat sinking functionality to the incubation mechanism.
More specifically: when the protective case 1000 is loaded, a three-dimensional schematic diagram of the immunoassay integrated system is shown in fig. 4.
The above description is only a preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent processes, which are made by using the contents of the present specification and the accompanying drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (10)
1. The utility model provides an immunoassay integrated system, a serial communication port, including the stake body that has first inner chamber, arrange the mechanism of hatching in first inner chamber in, set up in the fluorescence detection mechanism at stake body top, a two-dimensional code scanning module for scanning the two-dimensional code on the test card, a process transfer mechanism for carrying out the process transfer to the test card, be provided with on the fluorescence detection mechanism and be used for test card male test mouth, be provided with a plurality of incubation groove that can be used to the test card to put into on incubation mechanism's the preceding terminal surface, a plurality of incubation groove are the linear arrangement in the vertical direction.
2. The immunoassay integrated system of claim 1, further comprising a substrate, wherein the holder body and the process transfer mechanism are disposed on the substrate.
3. The immunoassay integrated system of claim 1, wherein the process transfer mechanism comprises a lifting platform, a screw rod connected with the lifting platform, a horizontal guide rail arranged on the lifting platform, a card-shifting solenoid valve, a horizontal card-shifting motor connected with the card-shifting solenoid valve, and a vertical lifting motor connected with the screw rod, wherein the card-shifting solenoid valve is used for pushing the test card from the horizontal guide rail into the incubation groove or the test port.
4. The integrated immunoassay system of claim 3, wherein the horizontal guide rail is provided with a sliding groove, one end of the sliding groove near the incubation groove is provided with an in-position detection mechanism for the test card in the cage, and one end of the sliding groove near the two-dimensional code scanning module is provided with a in-position detection mechanism for the dialing card.
5. The immunoassay integrated system of claim 2, further comprising an insertion mechanism provided with an insertion site for insertion of the test card, wherein the two-dimensional code scanning module is located above the insertion mechanism.
6. The immunoassay integration system of claim 5, further comprising a test card detection mechanism disposed on the insertion mechanism.
7. The immunoassay integrated system of claim 1, wherein the incubation mechanism comprises a main housing defining a second cavity, a heat sink for dissipating heat from the second cavity, a cooling system for cooling the second cavity, and a temperature control plate, the temperature control plate is connected to the cooling system, the incubation grooves are disposed on a front end surface of the main housing, and the incubation grooves are communicated with the second cavity.
8. The immunoassay integration system of claim 7, wherein the number of heat sinks is at least two.
9. The immunoassay integrated system of claim 8, wherein the second lumen has a heating module disposed therein that is electrically connected to the thermal control plate.
10. The integrated immunoassay system of claim 9, wherein the bottom of the main housing is provided with a condensed water drain port.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202110150265.XA CN112964638A (en) | 2021-02-03 | 2021-02-03 | Immunoassay integrated system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110150265.XA CN112964638A (en) | 2021-02-03 | 2021-02-03 | Immunoassay integrated system |
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| CN112964638A true CN112964638A (en) | 2021-06-15 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090021728A1 (en) * | 2007-06-21 | 2009-01-22 | Gen-Probe Incorporated | Multi-Channel Optical Measurement Instrument |
| CN108593949A (en) * | 2018-02-11 | 2018-09-28 | 金博特（北京）生物科技有限公司 | A kind of chemical illumination immunity analysis instrument |
| CN110007069A (en) * | 2019-03-05 | 2019-07-12 | 深圳迎凯生物科技有限公司 | Fluorescence immunoassay tester |
| CN209264568U (en) * | 2018-10-08 | 2019-08-16 | 北京智箭光电科技有限公司 | A kind of vertically oriented lift multichannel dry type fluorescence detector |
| CN111781344A (en) * | 2020-07-31 | 2020-10-16 | 重庆中元汇吉生物技术有限公司 | Fluorescence immunoassay appearance |
-
2021
- 2021-02-03 CN CN202110150265.XA patent/CN112964638A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090021728A1 (en) * | 2007-06-21 | 2009-01-22 | Gen-Probe Incorporated | Multi-Channel Optical Measurement Instrument |
| CN108593949A (en) * | 2018-02-11 | 2018-09-28 | 金博特（北京）生物科技有限公司 | A kind of chemical illumination immunity analysis instrument |
| CN209264568U (en) * | 2018-10-08 | 2019-08-16 | 北京智箭光电科技有限公司 | A kind of vertically oriented lift multichannel dry type fluorescence detector |
| CN110007069A (en) * | 2019-03-05 | 2019-07-12 | 深圳迎凯生物科技有限公司 | Fluorescence immunoassay tester |
| CN111781344A (en) * | 2020-07-31 | 2020-10-16 | 重庆中元汇吉生物技术有限公司 | Fluorescence immunoassay appearance |
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