CN112947224A - Based on crooked formula bioreactor intelligence control system - Google Patents
Based on crooked formula bioreactor intelligence control system Download PDFInfo
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- CN112947224A CN112947224A CN202110238954.6A CN202110238954A CN112947224A CN 112947224 A CN112947224 A CN 112947224A CN 202110238954 A CN202110238954 A CN 202110238954A CN 112947224 A CN112947224 A CN 112947224A
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- 238000006243 chemical reaction Methods 0.000 claims abstract description 133
- 238000005452 bending Methods 0.000 claims abstract description 60
- 238000007599 discharging Methods 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 102
- 239000007788 liquid Substances 0.000 claims description 57
- 239000000203 mixture Substances 0.000 claims description 50
- 239000003513 alkali Substances 0.000 claims description 34
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 30
- 229910052760 oxygen Inorganic materials 0.000 claims description 30
- 239000001301 oxygen Substances 0.000 claims description 30
- 239000002253 acid Substances 0.000 claims description 29
- 230000002572 peristaltic effect Effects 0.000 claims description 24
- 238000006073 displacement reaction Methods 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 16
- 239000000243 solution Substances 0.000 claims description 16
- 239000007789 gas Substances 0.000 claims description 14
- 238000004811 liquid chromatography Methods 0.000 claims description 13
- 230000001502 supplementing effect Effects 0.000 claims description 11
- 239000001963 growth medium Substances 0.000 claims description 9
- 238000004321 preservation Methods 0.000 claims description 9
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 5
- 230000005856 abnormality Effects 0.000 claims description 4
- 238000010521 absorption reaction Methods 0.000 claims description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 3
- 239000001569 carbon dioxide Substances 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 230000008021 deposition Effects 0.000 claims description 3
- 230000000249 desinfective effect Effects 0.000 claims description 3
- 238000002347 injection Methods 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 239000000575 pesticide Substances 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 238000005086 pumping Methods 0.000 claims description 3
- 238000004064 recycling Methods 0.000 claims description 3
- 238000005070 sampling Methods 0.000 claims description 3
- 239000010865 sewage Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 2
- 238000013480 data collection Methods 0.000 claims 1
- 238000004891 communication Methods 0.000 abstract description 2
- 238000010276 construction Methods 0.000 abstract description 2
- 238000013461 design Methods 0.000 abstract description 2
- 238000004904 shortening Methods 0.000 abstract description 2
- 238000003860 storage Methods 0.000 abstract description 2
- 230000004069 differentiation Effects 0.000 abstract 1
- 230000010354 integration Effects 0.000 abstract 1
- 238000003756 stirring Methods 0.000 description 7
- 238000010586 diagram Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000012544 monitoring process Methods 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000000151 deposition Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000012670 alkaline solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000003062 neural network model Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
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- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05B—CONTROL OR REGULATING SYSTEMS IN GENERAL; FUNCTIONAL ELEMENTS OF SUCH SYSTEMS; MONITORING OR TESTING ARRANGEMENTS FOR SUCH SYSTEMS OR ELEMENTS
- G05B19/00—Programme-control systems
- G05B19/02—Programme-control systems electric
- G05B19/04—Programme control other than numerical control, i.e. in sequence controllers or logic controllers
- G05B19/042—Programme control other than numerical control, i.e. in sequence controllers or logic controllers using digital processors
- G05B19/0423—Input/output
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- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05B—CONTROL OR REGULATING SYSTEMS IN GENERAL; FUNCTIONAL ELEMENTS OF SUCH SYSTEMS; MONITORING OR TESTING ARRANGEMENTS FOR SUCH SYSTEMS OR ELEMENTS
- G05B2219/00—Program-control systems
- G05B2219/20—Pc systems
- G05B2219/25—Pc structure of the system
- G05B2219/25257—Microcontroller
Abstract
The invention discloses an intelligent control system based on a bending bioreactor, which comprises a bending reaction tube, a jacket tube, a PID (proportion integration differentiation) controller, an A/D (analog/digital) conversion module and an instant data collector, wherein a temperature control device is arranged on the bending reaction tube, an air inlet is formed in the bending reaction tube, a first discharging valve is arranged on a discharging port, a second discharging valve is arranged on the discharging port, a third discharging valve is arranged on the discharging port, and a fourth discharging valve is arranged on the discharging port. The intelligent control system based on the bending bioreactor is provided with the PLC, the PLC is large in control scale, has perfect data operation capacity, is strong in communication capacity, is easy to interface, and is easy to accept by engineering technicians. The PLC uses the storage logic to replace the wiring logic, thereby greatly reducing the external wiring of the control equipment, greatly shortening the design and construction period of the control system and simultaneously being easy to maintain.
Description
Technical Field
The invention relates to the technical field of a bending bioreactor, in particular to an intelligent control system based on the bending bioreactor.
Background
With the development of process sensing technology and computer technology, new concept reaction systems for multi-scale research of biological reaction processes have become a trend of industry development. The method has the advantages of having more than ten or even more on-line parameter detection or control, being suitable for various reaction characteristics, fusing various process theories and control theories, being convenient for the process analysis and optimization operation of the reaction process, realizing the process optimization and software control, and being suitable for the improvement of the reactor body structure of the control system, and becoming the technology which is important breakthrough in the industry.
The biological reaction systems most used at home and abroad are a stirring type biological reactor and a control system thereof, a drum type biological reactor and a control system thereof, wherein the stirring type biological reactor is provided with a stirring device in a reaction tank, the stirring type biological reaction can generate shear stress, the cooling process is slow, and the heat in the middle of the material can not be transferred out, so that the inoculation efficiency is influenced. The drum-type biological reaction can not control the on-line liquid supply and is difficult to realize industrial scale. We propose an intelligent control system based on a curved bioreactor in order to solve the problems set forth above.
Disclosure of Invention
The invention aims to provide an intelligent control system based on a bending bioreactor, which aims to solve the problems that the stirring bioreactor provided by the background technology is provided with a stirring device in a reaction tank, the stirring bioreactor can generate shear stress, the cooling process is slow, and the heat in the middle of the material cannot be transferred, so that the inoculation efficiency is influenced. The drum-type biological reaction can not control the on-line liquid supply and is difficult to realize the industrial scale.
In order to achieve the purpose, the invention provides the following technical scheme: an intelligent control system based on a bending bioreactor comprises a bending reaction tube, a jacketed tube, a PID controller, an A/D conversion module and an instant data acquisition device, wherein a temperature control device is arranged on the bending reaction tube, an air inlet is formed in the bending reaction tube, a discharge port is formed in the bending reaction tube, an acid and alkali supplementing port is formed in the bending reaction tube, an air outlet is formed in the bending reaction tube, a temperature measuring device is installed on the bending reaction tube, a PH value measuring device is installed on the bending reaction tube, a dissolved oxygen measuring device is arranged on the bending reaction tube, a feed inlet is formed in the bending reaction tube, a first air inlet valve is installed on the air inlet, a second air inlet valve is arranged on the air inlet, a third air inlet valve is formed in the air inlet, and a first discharge valve is installed on the discharge port, a second discharge valve is arranged on the discharge port, a third discharge valve is arranged on the discharge port, a fourth discharge valve is arranged on the discharge port, a first exhaust valve is arranged on the exhaust port, a second exhaust valve is arranged on the exhaust port, a third exhaust valve is arranged on the exhaust port, a water seal container is connected onto the third exhaust valve, an acid adding peristaltic pump is arranged on the acid and alkali supplementing port, an alkali adding peristaltic pump is arranged on the acid and alkali supplementing port, an acid solution bottle is connected onto the acid adding peristaltic pump, an alkali solution bottle is arranged on the alkali adding peristaltic pump, a heat preservation hot water tank is connected onto the jacket pipe, a cold water tank is arranged on the jacket pipe, a hot water inlet electromagnetic valve is arranged on the heat preservation hot water tank, a cold water inlet electromagnetic valve is arranged on the cold water tank, a hot water circulating pump is arranged on the hot water tank, and a cold water circulating pump is arranged on the cold water tank, the device comprises a bending reaction tube, a jacket tube, a communicating water pipe, a displacement pump, a liquid chromatography device, a turbidity sensing device, a bent tube part and a water outlet valve, wherein the jacket tube is wrapped on the bending reaction tube, the communicating water pipe is connected between the jacket tubes, the bending reaction tube is connected with the vertical tube part, the displacement pump is installed on the vertical tube part, the liquid chromatography device is arranged on the vertical tube part, the turbidity sensing device is installed on the vertical tube part, the bent tube part is connected between the bending reaction tube, and the water outlet valve is.
Preferably, the instant data collector is connected with a gas sensor, a temperature sensor, a pH value sensor, a dissolved oxygen sensor, a liquid chromatography sensor and a turbidity sensor, and is connected with the PID controller through an A/D conversion module.
Preferably, the PID controller is connected with the human-computer interface and the industrial personal computer, and the human-computer interface and the industrial personal computer are both connected with the PLC.
Preferably, the temperature control device, the air inlet, the discharge hole, the volumetric pump, the acid and alkali supplementing hole and the exhaust hole are controlled by a PLC (programmable logic controller).
The invention provides an intelligent control system based on a bending bioreactor, which comprises the following steps:
the method comprises the following steps: sterilizing the bent reaction tube; opening the first air inlet valve and the second air inlet valve, and introducing high-temperature steam to perform high-temperature sterilization and disinfection on the pipe wall of the bent reaction pipe and air; opening a first exhaust valve and a second exhaust valve to exhaust high-temperature steam into the atmosphere; opening the first discharging valve and the second discharging valve to discharge condensed water condensed in the closed high-temperature steam; meanwhile, the pipe wall is cleaned for the second time by using condensed water, and the condensed water or sewage is discharged through an outlet of the second discharge valve; closing the second air inlet valve and the second exhaust valve, and stopping inputting and exhausting high-temperature steam;
step two: cooling the bent reaction tube; opening a third air inlet valve and a third air exhaust valve, introducing sterile air through the third air inlet valve, and allowing redundant air to enter a water seal container through the third air exhaust valve to be exhausted so as to ensure the sealing property of the bent reaction tube; the high-temperature bending reaction tube is cooled to room temperature by utilizing the sterile air at the room temperature, when no liquid is discharged from the outlet of the second discharge valve, the first discharge valve and the second discharge valve are closed, and the sterile air is continuously introduced to carry out drying treatment on the tube wall; introducing room temperature water into the jacket pipe, and ensuring the temperature range of the water to be within 25-30 ℃;
step three: injecting a reaction solution; opening the feed inlet, uniformly mixing the sterile culture medium and the strains, and injecting the mixture into a bent reaction tube; the volume of the culture medium is not more than 90% of the total volume of the bent reaction tube, and the strains account for 1-5% of the total volume of the bent reaction tube; closing the feed inlet after the injection is finished;
step four: carrying out a shape-advancing biological reaction; opening a volumetric pump switch, slowly pumping the mixture liquid of the culture medium and the strains and the filled sterile air into the top of the bent reaction tube from the vertical tube part from the bottom of the bent reaction tube through the volumetric pump, and enabling the mixture liquid to flow from top to bottom through the bent tube part for circulation; the flow velocity of the mixture liquid in the bent reaction tube is less than or equal to 0.1 m/s by adjusting the displacement pump, so that the sterile air and the mixture liquid are fully combined, the absorption rate of oxygen is improved, and the problems of deposition and precipitation of the mixture liquid are prevented;
step five: sampling; opening the first discharge valve and the third discharge valve every 3-6 hours to sample the mixture liquid according to the biological reaction period, sending the mixture liquid to a relevant department to detect the reaction degree of the mixture liquid, and closing the first discharge valve and the third discharge valve;
step six: discharging; opening the first discharge valve, the fourth discharge valve and the second exhaust valve, taking out the mixed liquid in the bent reaction tube, and closing the third exhaust valve to prevent the water in the water seal container from flowing back into the bent reaction tube to pollute the mixed liquid;
step seven: completing the biological reaction; and after the mixture liquid is emptied, closing the first air inlet valve, the third air inlet valve, the first discharge valve, the fourth discharge valve, the first exhaust valve and the second exhaust valve, and cleaning and disinfecting the bent reaction tube.
Preferably, in the fourth step, the pH value of the mixture liquid in the pipe is monitored in real time through a pH value sensor arranged on a pH value measuring device, and acid and alkali are slowly supplemented through an acid adding peristaltic pump or an alkali adding peristaltic pump; in the process of biological reaction, the pH value is generally required to be 7.0, and when the mixture liquid is pesticide, the pH value is required to be 6.8; when the pH value of the mixture liquid is detected to be acid, the alkali-adding peristaltic pump is started, and an alkali solution is injected; and vice versa.
Preferably, in the fourth step, the mixture liquid is monitored in real time through a liquid chromatography device and a turbidity sensing device, and an alarm is given when an abnormality occurs.
Preferably, in the fourth step, a dissolved oxygen sensor is arranged on the dissolved oxygen measuring device to monitor the dissolved oxygen value of the mixture liquid in the tube in real time, and the oxygen content in the bent reaction tube is adjusted by increasing the flow rate of the displacement pump and/or accelerating the flow of the input sterile air; meanwhile, a gas component sensor is arranged on the exhaust port to judge gas component data of the exhaust gas, and when the proportion of the carbon dioxide is too high, the oxygen content in the bent reaction tube is adjusted by increasing the flow rate of the displacement pump and/or accelerating the flow of the input sterile air; similarly, when the oxygen content is too high, the flow rate of the volumetric pump is reduced and/or the input of sterile air is slowed.
Preferably, the temperature of the mixture liquid in the pipe and the water in the jacket pipe is monitored in real time through a temperature sensor arranged on the temperature measuring device in the fourth step, when the temperature is higher than the reaction temperature, a cold water inlet electromagnetic valve and a cold water circulating pump are opened, the water in the cold water tank is pumped into the jacket pipe, a water outlet valve is opened to discharge redundant water, and vice versa, and the discharged water can be injected into the cold water tank for water resource saving and recycling.
Compared with the prior art, the invention has the beneficial effects that: this based on crooked formula bioreactor intelligence control system:
(1) the PLC is arranged, the control scale of the PLC is large, the PLC has perfect data operation capacity, the communication capacity is strong, the interface is easy, and the programming language is easy to accept by engineering technicians. The PLC uses the storage logic to replace the wiring logic, thereby greatly reducing the external wiring of the control equipment, greatly shortening the design and construction period of the control system and simultaneously facilitating the maintenance;
(2) a multivariable self-adaptive fuzzy neural network model is adopted to learn, identify and process biological reaction parameter sets of acquired PH value, dissolved oxygen degree, temperature, gas composition, turbidity and the like, so that the dynamic characteristics of the complex and changeable field are adapted, and a displacement pump, a peristaltic pump and each valve are intelligently controlled.
Drawings
FIG. 1 is a schematic diagram of the PID control flow of an intelligent control system based on a bending bioreactor according to the present invention;
FIG. 2 is a schematic view of the parameter optimization process of an intelligent control system based on a bending bioreactor according to the present invention;
FIG. 3 is a schematic diagram of a parameter comparison analysis process of an intelligent control system based on a bending bioreactor according to the present invention;
FIG. 4 is a schematic process flow diagram of a control system based on an intelligent control system of a bending bioreactor according to the present invention;
FIG. 5 is a schematic diagram of a curved reaction tube structure based on an intelligent control system of a curved bioreactor.
In the figure: 1. a curved reaction tube; 2. a temperature control device; 3. an air inlet; 4. a discharge port; 5. a positive displacement pump; 6. acid and alkali supplement; 7. an exhaust port; 8. a temperature measuring device; 9. a pH value measuring device; 10. a dissolved oxygen measuring device; 11. a water seal container; 12. a feed inlet; 13. a first intake valve; 14. a second intake valve; 15. a third intake valve; 16. a first discharge valve; 17. a second discharge valve; 18. a third discharge valve; 19. a fourth discharge valve; 20. a first exhaust valve; 21. a second exhaust valve; 22. a third exhaust valve; 23. adding an acid peristaltic pump; 24. adding an alkali peristaltic pump; 25. an acid solution bottle; 26. an alkaline solution bottle; 27. a heat preservation hot water tank; 28. a cold water tank; 29. a hot water inlet solenoid valve; 30. a cold water inlet solenoid valve; 31. a liquid chromatography device; 32. a turbidity sensing device; 33. a hot water circulation pump; 34. a cold water circulation pump; 35. a jacket pipe; 36. is communicated with a water pipe; 37. a vertical pipe portion; 38. a bent tube portion; 39. a water outlet valve; 40. a gas sensor; 41. a temperature sensor; 42. a pH value sensor; 43. a dissolved oxygen sensor; 44. a liquid chromatography sensor; 45. a turbidity sensor; 46. an industrial personal computer; 47. a human-machine interface; 48. a PLC controller; 49. a PID controller; 50. an A/D conversion module; 51. an instant data collector.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example one
Referring to fig. 1-5, the present invention provides a technical solution: an intelligent control system based on a bending bioreactor comprises a bending reaction tube 1, a jacketed pipe 35, a PID controller 49, an A/D conversion module 50 and an instant data collector 51, wherein a temperature control device 2 is arranged on the bending reaction tube 1, an air inlet 3 is arranged on the bending reaction tube 1, a discharge port 4 is arranged on the bending reaction tube 1, an acid and alkali supplementing port 6 is arranged on the bending reaction tube 1, an exhaust port 7 is arranged on the bending reaction tube 1, a temperature measuring device 8 is arranged on the bending reaction tube 1, a PH value measuring device 9 is arranged on the bending reaction tube 1, a dissolved oxygen measuring device 10 is arranged on the bending reaction tube 1, a feed port 12 is arranged on the bending reaction tube 1, a first air inlet valve 13 is arranged on the air inlet 3, a second air inlet valve 14 is arranged on the air inlet 3, and a third air inlet valve 15 is arranged on the air inlet 3, a first discharge valve 16 is arranged on the discharge port 4, a second discharge valve 17 is arranged on the discharge port 4, a third discharge valve 18 is arranged on the discharge port 4, a fourth discharge valve 19 is arranged on the discharge port 4, a first exhaust valve 20 is arranged on the exhaust port 7, a second exhaust valve 21 is arranged on the exhaust port 7, a third exhaust valve 22 is arranged on the exhaust port 7, a water seal container 11 is connected on the third exhaust valve 22, an acid and alkali adding peristaltic pump 23 is arranged on the acid and alkali supplementing port 6, an alkali adding peristaltic pump 24 is arranged on the acid and alkali supplementing port 6, an acid solution bottle 25 is connected on the acid and alkali adding peristaltic pump 23, an alkali solution bottle 26 is arranged on the alkali adding peristaltic pump 24, a heat preservation hot water tank 27 is connected on the jacket 35, a cold water tank 28 is arranged on the jacket 35, a hot water inlet electromagnetic valve 29 is arranged on the heat preservation hot water tank 27, and a cold water inlet electromagnetic valve 30 is arranged on the cold, install hot water circulating pump 33 on the heat preservation hot-water tank 27, and be provided with cold water circulating pump 34 on the cold-water tank 28, the parcel has jacket pipe 35 on the crooked formula reaction tube 1, and be connected with UNICOM's water pipe 36 between the jacket pipe 35, be connected with vertical pipe portion 37 on the crooked formula reaction tube 1, and install displacement pump 5 on the vertical pipe portion 37, be provided with liquid chromatography device 31 on the vertical pipe portion 37, and install turbidity sensing device 32 on the vertical pipe portion 37, be connected with crooked pipe portion 38 between the crooked formula reaction tube 1, and set up outlet valve 39 on the jacket pipe 35.
The instant data collector 51 is connected with a gas sensor 40, a temperature sensor 41, a pH value sensor 42, a dissolved oxygen sensor 43, a liquid chromatography sensor 44 and a turbidity sensor 45, and the instant data collector 51 is connected with a PID controller 49 through an A/D conversion module 50.
The PID controller 49 is connected with the human-computer interface 47 and the industrial personal computer 46, and the human-computer interface 47 and the industrial personal computer 46 are both connected with the PLC controller 48.
The temperature control device 2, the air inlet 3, the discharge port 4, the displacement pump 5, the acid and alkali supplementing port 6 and the exhaust port 7 are controlled by a PLC 48.
Example two
Referring to fig. 1-5, the present invention provides a technical solution: an intelligent control system based on a bending bioreactor comprises the following steps:
the method comprises the following steps: sterilizing the bent reaction tube 1; opening a first air inlet valve 13 and a second air inlet valve 14, and introducing high-temperature steam to perform high-temperature sterilization and disinfection on the tube wall of the bent reaction tube 1 and air; opening a first exhaust valve 20 and a second exhaust valve 21 to discharge high-temperature steam into the atmosphere; opening a first discharge valve 16 and a second discharge valve 17 to discharge condensed water condensed in the closed high-temperature steam; meanwhile, the pipe wall is cleaned for the second time by using condensed water, and the condensed water or sewage is discharged through an outlet of the second discharge valve 17; the second intake valve 14 and the second exhaust valve 21 are closed, and the input of the high-temperature steam and the exhaust thereof are stopped;
step two: cooling the bent reaction tube 1; opening a third air inlet valve 15 and a third air outlet valve 22, introducing sterile air through the third air inlet valve 15, and discharging redundant air into the water seal container 11 through the third air outlet valve 22 to ensure the tightness of the bent reaction tube 1; the high-temperature bending reaction tube 1 is cooled to room temperature by utilizing the sterile air at the room temperature, when no liquid is discharged from the outlet of the second discharge valve 17, the first discharge valve 16 and the second discharge valve 17 are closed, and the sterile air is continuously introduced to dry the tube wall; introducing room temperature water into the jacketed pipe 35 to ensure that the temperature range of the water is within 25-30 ℃;
step three: injecting a reaction solution; opening the feed inlet 12, uniformly mixing the sterile culture medium and the strains, and injecting the mixture into the bent reaction tube 1; the volume of the culture medium is not more than 90% of the total volume of the bent reaction tube 1, and the strains account for 1-5% of the total volume of the bent reaction tube 1; closing the feed inlet 12 after the injection is finished;
step four: carrying out a shape-advancing biological reaction; opening a switch of the volumetric pump 5, slowly pumping the mixture liquid of the culture medium and the strains and the filled sterile air into the top of the curved reaction tube 1 from the vertical tube part 37 from the bottom of the curved reaction tube 1 through the volumetric pump 5, and enabling the mixture liquid to flow from top to bottom through the curved tube part 38 for circulation; adjusting the displacement pump 5 to ensure that the flow rate of the mixture liquid in the bent reaction tube 1 is less than or equal to 0.1 m/s, ensuring that the sterile air is fully combined with the mixture liquid, improving the absorption rate of oxygen, preventing the mixture liquid from deposition and precipitation, monitoring the pH value of the mixture liquid in the tube in real time through a pH value sensor 42 arranged on a pH value measuring device 9, and slowly supplementing acid and alkali through an acid adding peristaltic pump 23 or an alkali adding peristaltic pump 24; in the process of biological reaction, the pH value is generally required to be 7.0, and when the mixture liquid is pesticide, the pH value is required to be 6.8; when the pH value of the mixture liquid is detected to be acid, the alkali adding peristaltic pump 24 is started, and alkali solution is injected; and vice versa. The liquid chromatography device 31 and the turbidity sensing device 32 are used for monitoring the mixture liquid in real time and giving an alarm when an abnormality occurs. The liquid chromatography device 31 and the turbidity sensing device 32 are used for monitoring the mixture liquid in real time and giving an alarm when an abnormality occurs. The dissolved oxygen sensor 43 is arranged on the dissolved oxygen measuring device 10 to monitor the dissolved oxygen value of the mixture liquid in the pipe in real time, and the oxygen content in the bent reaction pipe 1 is adjusted by improving the flow rate of the displacement pump 5 and/or accelerating the flow of the input sterile air; meanwhile, a gas component sensor is arranged on the exhaust port 7 to judge gas component data of the exhaust gas, and when the proportion of the carbon dioxide is too high, the oxygen content in the bent reaction tube 1 is adjusted by increasing the flow rate of the displacement pump 5 and/or accelerating the flow of the input sterile air; similarly, when the oxygen content is too high, the flow rate of the volumetric pump 5 is reduced and/or the input of sterile air is slowed. The temperature of the mixture liquid in the pipe and the water in the jacket 35 are monitored in real time through a temperature sensor 41 arranged on the temperature measuring device 8, when the temperature is higher than the reaction temperature, a cold water inlet electromagnetic valve and a cold water circulating pump 34 are opened, the water in the cold water tank 28 is injected into the jacket 35, meanwhile, a water outlet valve 39 is opened to discharge the redundant water, and vice versa, and meanwhile, in order to save water resources, the discharged water can be injected into the cold water tank 28 for recycling;
step five: sampling; according to the biological reaction period, opening the first discharge valve 16 and the third discharge valve 18 every 3-6 hours to sample the mixture liquid, sending the mixture liquid to a relevant department to detect the reaction degree of the mixture liquid, and closing the first discharge valve 16 and the third discharge valve 18;
step six: discharging; opening a first discharge valve 16, a fourth discharge valve 19 and a second exhaust valve 21, taking out the mixed liquid in the bent reaction tube 1, and closing a third exhaust valve 22 to prevent water in the water seal container 11 from flowing back into the bent reaction tube 1 to pollute the mixed liquid;
step seven: completing the biological reaction; and after the mixture liquid is emptied, closing the first air inlet valve 13, the third air inlet valve 15, the first discharge valve 16, the fourth discharge valve 19, the first exhaust valve 20 and the second exhaust valve 21, and cleaning and disinfecting the bent reaction tube 1.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.
Claims (9)
1. The utility model provides a based on crooked formula bioreactor intelligence control system, includes crooked formula reaction tube (1), jacketed pipe (35), PID controller (49), AD conversion module (50) and instant data collection station (51), its characterized in that: the device is characterized in that a temperature control device (2) is arranged on the bending reaction tube (1), an air inlet (3) is formed in the bending reaction tube (1), a discharge port (4) is formed in the bending reaction tube (1), an acid and alkali supplementing port (6) is formed in the bending reaction tube (1), an exhaust port (7) is formed in the bending reaction tube (1), a temperature measuring device (8) is installed on the bending reaction tube (1), a PH value measuring device (9) is installed on the bending reaction tube (1), a dissolved oxygen measuring device (10) is arranged on the bending reaction tube (1), a feed inlet (12) is formed in the bending reaction tube (1), a first air inlet valve (13) is installed on the air inlet (3), a second air inlet valve (14) is arranged on the air inlet (3), and a third air inlet valve (15) is formed in the air inlet (3), a first discharge valve (16) is installed on the discharge port (4), a second discharge valve (17) is arranged on the discharge port (4), a third discharge valve (18) is arranged on the discharge port (4), a fourth discharge valve (19) is installed on the discharge port (4), a first exhaust valve (20) is arranged on the exhaust port (7), a second exhaust valve (21) is installed on the exhaust port (7), a third exhaust valve (22) is arranged on the exhaust port (7), a water seal container (11) is connected onto the third exhaust valve (22), an acid-adding peristaltic pump (23) is installed on the acid-adding alkali port (6), an alkali-adding peristaltic pump (24) is arranged on the acid-adding alkali port (6), an acid solution bottle (25) is connected onto the acid-adding peristaltic pump (23), and an alkali solution bottle (26) is arranged on the alkali-adding peristaltic pump (24), the device is characterized in that a heat-preservation hot water tank (27) is connected onto the jacketed pipe (35), a cold water tank (28) is arranged on the jacketed pipe (35), a hot water inlet electromagnetic valve (29) is installed on the heat-preservation hot water tank (27), a cold water inlet electromagnetic valve (30) is arranged on the cold water tank (28), a hot water circulating pump (33) is installed on the heat-preservation hot water tank (27), a cold water circulating pump (34) is arranged on the cold water tank (28), the curved reaction pipe (1) is wrapped by the jacketed pipe (35), a communicated water pipe (36) is connected between the jacketed pipes (35), the curved reaction pipe (1) is connected with a vertical pipe part (37), a displacement pump (5) is installed on the vertical pipe part (37), a liquid chromatography device (31) is arranged on the vertical pipe part (37), and a turbidity sensing device (32) is installed on the vertical pipe part (37), a bent pipe part (38) is connected between the bent reaction pipes (1), and a water outlet valve (39) is arranged on the jacketed pipe (35).
2. The intelligent control system based on the bending bioreactor as claimed in claim 1, wherein: the real-time data acquisition unit (51) is connected with a gas sensor (40), a temperature sensor (41), a PH value sensor (42), a dissolved oxygen sensor (43), a liquid chromatography sensor (44) and a turbidity sensor (45), and the real-time data acquisition unit (51) is connected with a PID controller (49) through an A/D conversion module (50).
3. The intelligent control system based on the bending bioreactor as claimed in claim 1, wherein: and the PID controller (49) is connected with the human-computer interface (47) and the industrial personal computer (46), and the human-computer interface (47) and the industrial personal computer (46) are both connected with the PLC controller (48).
4. The intelligent control system based on the bending bioreactor as claimed in claim 1, wherein: the temperature control device (2), the air inlet (3), the discharge hole (4), the displacement pump (5), the acid and alkali supplementing hole (6) and the exhaust hole (7) are controlled by a PLC (programmable logic controller) 48.
5. The intelligent control system based on the bending bioreactor according to claim 1, wherein the steps comprise:
the method comprises the following steps: sterilizing the bent reaction tube (1); opening a first air inlet valve (13) and a second air inlet valve (14), and introducing high-temperature steam to perform high-temperature sterilization and disinfection on the tube wall of the bent reaction tube (1) and air; opening a first exhaust valve (20) and a second exhaust valve (21) to exhaust high-temperature steam into the atmosphere; opening a first discharge valve (16) and a second discharge valve (17) to discharge condensed water condensed in the closed high-temperature steam; meanwhile, the pipe wall is cleaned for the second time by using condensed water, and the condensed water or sewage is discharged through an outlet of a second discharge valve (17); closing the second air inlet valve (14) and the second exhaust valve (21), stopping the input of the high-temperature steam and the exhaust thereof;
step two: cooling the bent reaction tube (1); opening a third air inlet valve (15) and a third air exhaust valve (22), introducing sterile air through the third air inlet valve (15), and allowing redundant air to enter a water seal container (11) through the third air exhaust valve (22) to be exhausted, so that the sealing performance of the bent reaction tube (1) is ensured; the high-temperature bending reaction tube (1) is cooled to room temperature by utilizing the sterile air at room temperature, when no liquid is discharged from the outlet of the second discharge valve (17), the first discharge valve (16) and the second discharge valve (17) are closed, and the sterile air is continuously introduced to dry the tube wall; introducing room temperature water into the jacketed pipe (35) to ensure that the temperature range of the water is within 25-30 ℃;
step three: injecting a reaction solution; opening the feed inlet (12), uniformly mixing the sterile culture medium and the strains, and injecting the mixture into the bent reaction tube (1); the volume of the culture medium is not more than 90% of the total volume of the bent reaction tube (1), and the strains account for 1-5% of the total volume of the bent reaction tube (1); closing the feed inlet (12) after the injection is finished;
step four: carrying out a shape-advancing biological reaction; opening a switch of the volumetric pump (5), slowly pumping the mixture liquid of the culture medium and the strains and the flushed sterile air into the top of the curved reaction tube (1) from the bottom of the curved reaction tube (1) from the vertical tube part (37) through the volumetric pump (5), and enabling the mixture liquid to flow from top to bottom through the curved tube part (38) for circulation; the flow speed of the mixture liquid in the bent reaction tube (1) is less than or equal to 0.1 m/s by adjusting the displacement pump (5), so that the sterile air and the mixture liquid are fully combined, the absorption rate of oxygen is improved, and the problems of deposition and precipitation of the mixture liquid are prevented;
step five: sampling; according to the biological reaction period, opening a first discharge valve (16) and a third discharge valve (18) every 3-6 hours to sample the mixture liquid, sending the mixture liquid to a relevant department to detect the reaction degree of the mixture liquid, and closing the first discharge valve (16) and the third discharge valve (18);
step six: discharging; opening a first discharge valve (16), a fourth discharge valve (19) and a second exhaust valve (21), taking out the mixed liquid in the bent reaction tube (1), and closing a third exhaust valve (22) to prevent water in the water seal container (11) from flowing back into the bent reaction tube (1) to pollute the mixed liquid;
step seven: completing the biological reaction; and after the mixture liquid is emptied, closing the first air inlet valve (13), the third air inlet valve (15), the first discharge valve (16), the fourth discharge valve (19), the first exhaust valve (20) and the second exhaust valve (21), and cleaning and disinfecting the bent reaction tube (1).
6. The intelligent control system based on the bending bioreactor as claimed in claim 5, wherein: in the fourth step, the PH value of the mixture liquid in the pipe is monitored in real time through a PH value sensor (42) arranged on a PH value measuring device (9), and acid and alkali are slowly supplemented through an acid adding peristaltic pump (23) or an alkali adding peristaltic pump (24); in the process of biological reaction, the pH value is generally required to be 7.0, and when the mixture liquid is pesticide, the pH value is required to be 6.8; when the PH value of the mixture liquid is detected to be acid, the alkali adding peristaltic pump (24) is started, and alkali solution is injected; and vice versa.
7. The intelligent control system based on the bending bioreactor as claimed in claim 5, wherein: and in the fourth step, the mixture liquid is monitored in real time through a liquid chromatography device (31) and a turbidity sensing device (32), and an alarm is given when abnormality occurs.
8. The intelligent control system based on the bending bioreactor as claimed in claim 5, wherein: in the fourth step, a dissolved oxygen sensor (43) is arranged on the dissolved oxygen measuring device (10) to monitor the dissolved oxygen value of the mixture liquid in the tube in real time, and the oxygen content in the bent reaction tube (1) is adjusted by increasing the flow speed of the displacement pump (5) and/or accelerating the flow of the input sterile air; meanwhile, a gas component sensor is arranged on the exhaust port (7) to judge gas component data of the exhaust gas, and when the proportion of the carbon dioxide is too high, the oxygen content in the bent reaction tube (1) is adjusted by increasing the flow rate of the volumetric pump (5) and/or accelerating the flow of the input sterile air; similarly, when the oxygen content is too high, the flow rate of the volumetric pump (5) is reduced and/or the input of sterile air is slowed down.
9. The intelligent control system based on the bending bioreactor as claimed in claim 5, wherein: in the fourth step, the temperature of the mixture liquid in the pipe and the water in the jacket pipe (35) are monitored in real time through the temperature sensor (41) arranged on the temperature measuring device (8), when the temperature is higher than the reaction temperature, the cold water inlet electromagnetic valve and the cold water circulating pump (34) are opened, the water in the cold water tank (28) is pumped into the jacket pipe (35), the water outlet valve (39) is opened to discharge redundant water, and vice versa, and meanwhile, in order to save water resources, the discharged water can be injected into the cold water tank (28) for recycling.
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