CN112916057A - Liquid sample processing container - Google Patents
Liquid sample processing container Download PDFInfo
- Publication number
- CN112916057A CN112916057A CN201911240529.XA CN201911240529A CN112916057A CN 112916057 A CN112916057 A CN 112916057A CN 201911240529 A CN201911240529 A CN 201911240529A CN 112916057 A CN112916057 A CN 112916057A
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- Prior art keywords
- functional
- liquid
- cabin
- fluid valve
- compartment
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/52—Containers specially adapted for storing or dispensing a reagent
- B01L3/527—Containers specially adapted for storing or dispensing a reagent for a plurality of reagents
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4022—Concentrating samples by thermal techniques; Phase changes
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Clinical Laboratory Science (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a liquid sample processing container, which belongs to the technical field of life science, medical inspection and quarantine. Comprises a functional cabin, a sealing cover and a fluid valve; the functional cabin consists of at least two cabin bodies and contains different types of liquid; a liquid channel is arranged between the functional bins; the sealing cover is used for preventing liquid in the functional compartment from volatilizing and overflowing; the fluid valve is used for controlling the on-off state of the liquid channel between the functional chambers. All reagents and samples used in the experimental process are added into the functional cabins, and after the functional cabins are covered by the sealing covers, the liquid samples move among the different functional cabins in the sealing state by adjusting the opening and closing states of the fluid valves and utilizing the centrifugal force of the centrifugal machine. When improving experiment operating efficiency, effectively avoid letting the liquid sample expose in the air in the experimentation and lead to the risk of polluting.
Description
Technical Field
The invention relates to a liquid sample processing container, which belongs to the technical field of life science, medical inspection and quarantine.
Background
In extraction experiments of DNA, RNA, protein, and the like, a series of operations such as lysis of tissue cells, separation, washing, and purification of a target substance are involved. Whether operated manually or by means of automated equipment, it is necessary to transfer liquid samples and various reagents between multiple test tubes. Need switch the test tube lid repeatedly to a plurality of test tubes in the experimentation, not only seriously influence work efficiency, still can lead to cross contamination between the detected object sample because the volatilizing of liquid, or the pollution of sample to experimental environment.
Disclosure of Invention
The present invention addresses the above-described deficiencies and provides a liquid sample processing container.
The technical scheme for solving the technical problems is as follows:
a liquid sample processing container comprises a functional cabin, a sealing cover and a fluid valve; the functional cabin consists of at least two cabin bodies and contains different types of liquid; a liquid channel is arranged between the functional bins; the sealing cover is used for preventing liquid in the functional compartment from volatilizing and overflowing; the fluid valve is used for controlling the on-off state of the liquid channel between the functional chambers. The centrifugal force generated by the centrifuge drives the liquid to be transferred among the plurality of functional compartments.
The invention has the beneficial effects that: all reagents and samples used in the experimental process are added into the functional cabins, and after the functional cabins are covered by the sealing covers, the liquid samples move among the different functional cabins in the sealing state by adjusting the opening and closing states of the fluid valves and utilizing the centrifugal force of the centrifugal machine. When improving experiment operating efficiency, effectively avoid letting the liquid sample expose in the air in the experimentation and lead to the risk of polluting.
On the basis of the technical scheme, the invention can be further improved as follows.
And furthermore, a sealing ring is arranged on the fluid valve, and a groove is formed in the corresponding position on the functional cabin.
The beneficial effect of adopting the further scheme is that: the sealing ring can prevent liquid from overflowing from the fluid valve.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a cross-sectional view of the present invention.
In the figure, 1, a fluid valve a; 2. a functional cabin A; 3. a functional cabin B; 4. a functional cabin C; 5. a sealing cover; 6. a functional cabin D; 7. a functional compartment E; 8. a functional compartment F; 9. a fluid valve A; 10. a liquid channel A; 11. and a liquid channel B.
Detailed Description
The material of the container of the present invention comprises at least one material, including but not limited to polyethylene, polyoxymethylene, polyamide, polypropylene, polycarbonate, polystyrene, polymethacrylate.
The functional compartments may be arranged in different shapes, such as cylindrical, elliptic cylindrical, prismatic or other irregular shapes. The liquid channels communicated between the functional compartments can also be arranged in different shapes, such as a linear shape, a circular arc shape or an irregular shape; in certain embodiments, the cross-sectional area of the flow path is tapered.
The principles and features of this invention are described below in conjunction with the following drawings, which are set forth by way of illustration only and are not intended to limit the scope of the invention.
A liquid sample processing container as shown in FIG. 1 is used for processing a liquid sample to perform purification and extraction of a nucleic acid sample.
The container comprises a functional cabin D (6) and a functional cabin F (8) for adding cleaning fluid; a functional compartment E (7) for adding an eluent; the functional cabins are respectively communicated with a functional cabin C (4) for biochemical reaction through a liquid channel A (10).
The functional cabin C (4) is respectively communicated with a functional cabin B (3) for storing waste liquid after biochemical reaction and a functional cabin A (2) for storing purified nucleic acid through a liquid channel B (11).
A fluid valve A (1) for controlling the blocking or opening of the liquid channel A (10) is arranged between the functional cabin C (4) and the functional cabin D (6), between the functional cabin F (8) and between the functional cabin E (7);
a fluid valve B (9) for controlling the blocking or opening of the liquid channel B (11) is arranged between the functional cabin C (4) and the functional cabin B (3) and between the functional cabin A (2);
the sealing cover (5) can be opened and closed and is used for sealing the liquid added into each functional cabin.
The working principle of the invention is as follows:
step 1, adding all liquids involved in the nucleic acid extraction experiment
Confirming that the fluid valve A (1) and the fluid valve B (9) are in a closed state; cleaning liquid is added into the functional cabin D (6) and the functional cabin F (8); adding eluent into the functional compartment E (7); adding lysis solution, magnetic beads and samples into the functional cabin C (4) respectively; and all the functional cabins are tightly covered by a sealing cover (5).
And (3) putting the container into a water bath kettle or a metal bath for experiment, incubating for a period of time, completing biochemical reaction in the functional cabin C (4), cracking the sample, and adsorbing corresponding target nucleic acid onto magnetic beads.
An external magnetic field is applied to the bottom of the functional cabin C (4) for adsorbing the magnetic beads fixedly attached with the nucleic acid. The fluid valve B (9) is rotated by applying a mechanical external force to open the liquid passage B (11) between the functional compartment B (3) and the functional compartment C (4). The container of the present invention is placed in a centrifuge, and by applying a centrifugal force in the horizontal direction, the liquid in the functional compartment C (4) is driven to move entirely into the functional compartment B (3). The magnetic beads with the nucleic acids attached thereto are retained in the functional compartment C (4) by the magnetic force.
The liquid passage B (11) between the functional compartment B (3) and the functional compartment C (4) is closed by rotating the fluid valve B (9) by applying a mechanical external force. The external magnetic field applied at the bottom of the functional compartment C (4) is removed to release the magnetic beads. The fluid valve A (1) is rotated by applying a mechanical external force to open the liquid passage A (10) between the functional compartment D (6) and the functional compartment C (4). The container of the present invention is placed in a centrifuge, and by applying a centrifugal force in the horizontal direction, the cleaning solution in the functional compartment D (6) is driven to move all over to the functional compartment C (4), and the magnetic beads to which nucleic acids are attached are washed and cleaned.
And (3) repeating the step (3) after the cleaning is finished, and discharging waste liquid.
The liquid passage B (11) between the functional compartment B (3) and the functional compartment C (4) is closed by rotating the fluid valve B (9) by applying a mechanical external force. The external magnetic field applied at the bottom of the functional compartment C (4) is removed to release the magnetic beads. The fluid valve a (1) is rotated by applying a mechanical external force to open the liquid passage a (10) between the functional compartment F (8) and the functional compartment C (4). The container of the present invention is placed in a centrifuge, and by applying a centrifugal force in the horizontal direction, the whole of the cleaning solution in the functional compartment F (8) is driven to move into the functional compartment C (4), and the magnetic beads to which nucleic acids are attached are washed and cleaned.
And (3) repeating the step (3) after the cleaning is finished, and discharging waste liquid.
The liquid passage B (11) between the functional compartment B (3) and the functional compartment C (4) is closed by rotating the fluid valve B (9) by applying a mechanical external force. The external magnetic field applied at the bottom of the functional compartment C (4) is removed to release the magnetic beads. The fluid valve A (1) is rotated by applying a mechanical external force to open the liquid passage A (10) between the functional compartment E (7) and the functional compartment C (4). The container of the present invention is placed in a centrifuge, and by applying a centrifugal force in the horizontal direction, the whole of the eluate in the functional compartment E (7) is driven to move into the functional compartment C (4), and the magnetic beads to which nucleic acids are attached are washed and eluted. After the centrifugation is finished, the container is placed into a water bath or a metal bath, and the elution effect is enhanced by incubation and heating, so that the nucleic acid is thoroughly separated from the magnetic beads.
The liquid passage B (11) between the functional compartment a (2) and the functional compartment C (4) is opened by rotating the fluid valve B (9) by applying a mechanical external force. An external magnetic field is applied to the bottom of the functional cabin C (4) and is used for adsorbing the magnetic beads after the nucleic acid is fixed and eluted. The container is placed in a centrifuge, and the purified nucleic acid solution separated from the magnetic beads in the functional compartment C (4) is driven to move into the functional compartment A (2) by applying a centrifugal force in the horizontal direction. The magnetic beads after nucleic acid elution are retained in the functional compartment C (4) under the action of magnetic force.
The sealing cover (5) on the functional compartment A (2) is opened, and the purified nucleic acid is extracted for other experiments.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (2)
1. A liquid sample processing container, comprising: comprises a functional cabin, a sealing cover and a fluid valve; the functional cabin consists of at least two cabin bodies and contains different types of liquid; a liquid channel is arranged between the functional bins; the sealing cover is used for preventing liquid in the functional compartment from volatilizing and overflowing; the fluid valve is used for controlling the on-off state of the liquid channel between the functional chambers; and the centrifugal force of the centrifugal machine is utilized to realize that the liquid sample moves among different functional cabins in a sealed state.
2. A liquid sample processing container according to claim 1, wherein: and a sealing ring is arranged on the fluid valve, and a groove is arranged at a corresponding position on the functional cabin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911240529.XA CN112916057A (en) | 2019-12-06 | 2019-12-06 | Liquid sample processing container |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911240529.XA CN112916057A (en) | 2019-12-06 | 2019-12-06 | Liquid sample processing container |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112916057A true CN112916057A (en) | 2021-06-08 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911240529.XA Pending CN112916057A (en) | 2019-12-06 | 2019-12-06 | Liquid sample processing container |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112916057A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3595086A (en) * | 1968-11-21 | 1971-07-27 | Hoffmann La Roche | Extraction and storage arrangement |
| US20170153264A1 (en) * | 2013-03-14 | 2017-06-01 | Gen-Probe Incorporated | Method of using multi-well tray |
| CN211514563U (en) * | 2019-12-06 | 2020-09-18 | 青岛益柏生物科技有限公司 | Liquid sample processing container |
-
2019
- 2019-12-06 CN CN201911240529.XA patent/CN112916057A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3595086A (en) * | 1968-11-21 | 1971-07-27 | Hoffmann La Roche | Extraction and storage arrangement |
| US20170153264A1 (en) * | 2013-03-14 | 2017-06-01 | Gen-Probe Incorporated | Method of using multi-well tray |
| CN211514563U (en) * | 2019-12-06 | 2020-09-18 | 青岛益柏生物科技有限公司 | Liquid sample processing container |
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