CN112899153A - Leukemia cell separation and extraction device - Google Patents
Leukemia cell separation and extraction device Download PDFInfo
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- CN112899153A CN112899153A CN202110429269.1A CN202110429269A CN112899153A CN 112899153 A CN112899153 A CN 112899153A CN 202110429269 A CN202110429269 A CN 202110429269A CN 112899153 A CN112899153 A CN 112899153A
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- barrel body
- leukemia cell
- extraction device
- driving motor
- filter screen
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- 238000000926 separation method Methods 0.000 title claims abstract description 29
- 208000032839 leukemia Diseases 0.000 title claims abstract description 19
- 238000000605 extraction Methods 0.000 title claims abstract description 13
- 238000007789 sealing Methods 0.000 claims abstract description 20
- 239000007788 liquid Substances 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000003365 glass fiber Substances 0.000 claims abstract description 12
- 239000004065 semiconductor Substances 0.000 claims abstract description 10
- 239000007924 injection Substances 0.000 claims abstract description 9
- 238000002347 injection Methods 0.000 claims abstract description 9
- 238000005057 refrigeration Methods 0.000 claims abstract description 7
- 210000004027 cell Anatomy 0.000 claims description 23
- 210000005087 mononuclear cell Anatomy 0.000 claims description 6
- 210000003743 erythrocyte Anatomy 0.000 claims description 5
- 230000005855 radiation Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 210000004698 lymphocyte Anatomy 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000001772 blood platelet Anatomy 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000003480 eluent Substances 0.000 description 2
- 230000017525 heat dissipation Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 101150114468 TUB1 gene Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000023715 cellular developmental process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000000366 juvenile effect Effects 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/20—Heating or cooling
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- Engineering & Computer Science (AREA)
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- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Clinical Laboratory Science (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a leukemia cell separating and extracting device, which comprises a barrel body, wherein a machine case is arranged at the bottom of the barrel body, a grab handle is arranged at the bottom of the machine case, a liquid injection port and a liquid discharge port are respectively arranged at the upper end and the lower end of one side wall of the barrel body, a sealing cover is arranged at the top of the barrel body, a control panel is arranged at the other side wall of the barrel body, a discharge port is arranged at the bottom of the barrel body, a detachable battery is arranged inside the grab handle, a driving motor is arranged inside the driving motor, a water tank, a secondary separation cavity and a filter screen are arranged on one side of the inside of the barrel body from outside to inside, a semiconductor refrigeration sheet is arranged at the bottom of an inner cavity of the water tank, a glass fiber column layer is connected to the bottom of the sealing cover through a connecting rod, a temperature sensor is arranged on, and a temperature-controllable water tank is arranged outside the separation cavity, so that the activity of the cells after separation and extraction is ensured when the cells are separated and extracted.
Description
Technical Field
The invention relates to the technical field of cell extraction, in particular to a leukemia cell separating and extracting device.
Background
Leukemia is a malignant clonal disease of hematopoietic stem cells, leading to a series of syndromes caused by the cessation of cellular development in primitive and juvenile stages, and is caused by: one such chemical is benzene, which induces leukemia. In the case of newly decorated houses in homes and schools, and in the case of desks and chemicals, leukemia may be induced. The most common thing in life is to utilize horizontal centrifuge to carry out this operation, however, the device structure is complicated, is difficult for carrying, and is not very convenient during the use, and traditional cell separation extraction element is when carrying out blood separation, and blood separation is not enough, defects such as be not convenient for operate.
Based on the above, the invention designs a leukemia cell separating and extracting device to solve the above mentioned problems.
Disclosure of Invention
The invention aims to provide a leukemia cell separating and extracting device to solve the problems mentioned above.
In order to achieve the purpose, the invention provides the following technical scheme: a leukemia cell separating and extracting device comprises a barrel body, a machine case is installed at the bottom of the barrel body, a grab handle is installed at the bottom of the machine case, a liquid injection port and a liquid discharge port are respectively arranged at the upper end and the lower end of one side wall of the barrel body, a sealing cover is arranged at the top of the barrel body, a control panel is installed on the other side wall of the barrel body, a discharge port is arranged at the bottom of the barrel body, a detachable battery is installed inside the grab handle, a driving motor is installed inside the driving motor, a water tank, a secondary separation cavity and a filter screen are arranged inside the barrel body from outside to inside, the liquid injection port and the liquid discharge port are respectively communicated with the water tank, a semiconductor refrigeration sheet is installed at the bottom of an inner cavity of the water tank, the output end of the driving motor is connected with, the bottom of sealed lid is connected with the glass fiber column layer through the connecting rod, the glass fiber column layer is located the inside of filter screen, temperature sensor is installed to the outer wall of staving, the battery is connected with control panel, driving motor, temperature sensor and semiconductor refrigeration piece respectively.
Preferably, a heat dissipation window is formed on the outer wall of the case.
Preferably, the outer wall of the grab handle is provided with anti-skid grains.
Preferably, the both ends of sealed lid be equipped with the joint piece that the staving top is connected, the bottom of sealed lid is equipped with the sealing washer, the bottom ring channel of sealing washer, filter screen sliding connection is in the ring channel.
Preferably, the top of the sealing cover is provided with a pull ring.
Preferably, the diameter of the filter holes of the filter screen is smaller than the minimum diameter of the mononuclear cells and larger than the maximum diameter of the red blood cells.
Compared with the prior art, the invention has the beneficial effects that: this kind of leukemia cell separation extraction element novel in design, the structure is small and exquisite, and the portability is strong, can carry out the multiform separation to the leukemia cell and draw, and common EP pipe or heparin tube are collected the cell, and it is very convenient to use the operation, sets up the controllable basin of temperature in the separation chamber outside moreover for guarantee the activity after the cell separation draws when carrying out the separation to the cell and drawing.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the internal structure of the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
1. a barrel body; 2. a chassis; 3. a handle; 4. a liquid injection port; 5. a liquid discharge port; 6. a sealing cover; 7. a control panel; 8. a discharge outlet; 9. a heat dissipation window; 10. a battery; 11. a drive motor; 12. a water tank; 13. a clamping block; 14. a seal ring; 15. a secondary separation chamber; 16. a filter screen; 17. a drive shaft; 18. a connecting rod; 19. a glass fiber column layer; 20. a temperature sensor; 21. semiconductor refrigeration piece.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-2, the present invention provides a technical solution: a leukemia cell separating and extracting device comprises a barrel body 1, a case 2 is installed at the bottom of the barrel body 1, a grab handle 3 is installed at the bottom of the case 2, a liquid injection port 4 and a liquid discharge port 5 are respectively arranged at the upper end and the lower end of a side wall of the barrel body 1, a sealing cover 6 is arranged at the top of the barrel body 1, a control panel 7 is installed on another side wall of the barrel body 1, a discharge port 8 is arranged at the bottom of the barrel body 1, a detachable battery 10 is installed inside the grab handle 3, the driving motor 11 is installed inside a driving motor 11, a water tank 12, a secondary separation cavity 15 and a filter screen 16 are arranged inside the barrel body 1 from outside to inside, the liquid injection port 4 and the liquid discharge port 5 are respectively communicated with the water tank 12, a semiconductor refrigerating sheet 21 is installed at the bottom of an inner cavity of the water tank 12, and an output end of the, the bin outlet 8 with the secondary separation chamber 15 is linked together, the bottom of sealed lid 6 is connected with glass fiber column layer 19 through connecting rod 18, glass fiber column layer 19 is located the inside of filter screen 16, temperature sensor 20 is installed to the outer wall of staving 1, battery 10 is connected with control panel 7, driving motor 11, temperature sensor 20 and semiconductor refrigeration piece 21 respectively.
The outer wall of the case 2 is provided with a heat radiation window 9, so that the service life of a drive motor 11 in the case 2 is prolonged.
The anti-slip lines are arranged on the outer wall of the grab handle 3, friction force between the anti-slip lines and the hand is increased, the device is convenient to manually hold, and portability is high.
Wherein, the both ends of sealed lid 6 be equipped with the joint piece 13 that the staving 1 top is connected for this sealed lid 6 installation and dismantlement are convenient, the bottom of sealed lid 6 is equipped with sealing washer 14, the bottom ring channel of sealing washer 14, filter screen 16 sliding connection is in the ring channel, and this sealed lid 6 can realize sealing when using staving 1, does not influence the rotation of filter screen 16 moreover. The top of the sealing cover 6 is provided with a pull ring, so that the sealing cover 6 can be conveniently detached.
Wherein, the filtering hole diameter of the filtering screen 16 is smaller than the minimum diameter of the mononuclear cell and larger than the maximum diameter of the red blood cell, and is used for retaining the mononuclear cell in the filtering screen 16, and the red blood cell and the cell with smaller platelet diameter are collected in the secondary separation cavity 15.
The specific working principle is as follows:
when in use, the sealing cover 6 is opened, the cell sap to be separated and extracted is poured into the barrel body 1, then the sealing cover is closed, the operation is carried out through the control panel 7, the driving motor 11 is started, the cell sap is centrifugally separated by the driving shaft 17 through the driving motor 11, the centrifugal speed is controlled at 1000rpm, red blood cells and platelet cells passing through the filter screen 16 are collected through the discharge port 8, the lymphocytes are adsorbed on the glass fiber column layer 19, when the lymphocytes are adsorbed on the glass fiber column layer 19, the lymphocytes adsorbed on the glass fiber column layer 19 can be separated after the sealing cover 6 is opened, then unfiltered mononuclear cells in the separation cavity are poured out, the mononuclear cells are extracted, observed and researched, the multiple separation and extraction of the cells are realized, the operation is convenient, and by adding physiological saline into the barrel body 1, cleaning the column, adding eluent after cleaning, dissolving the lymphocyte adsorbed on the glass fiber column layer 19 in the eluent, and the use and replacement are convenient, in addition, the water tank 12 is arranged outside the separation cavity, the water change of the water tank 12 can be realized through the liquid injection port 4 and the liquid discharge port 5, in addition, the temperature sensor 20 monitors the environmental temperature in the water tank 12, the data is transmitted to the microprocessor (the single chip microcomputer stm32) of the control panel 7, the microprocessor determines whether to start the semiconductor refrigerating sheet 21 through the controller according to the preset temperature after analysis, if the temperature in the barrel body 1 exceeds a preset value, the controller starts the semiconductor refrigerating sheet 21 to cool the water tank 12, when the temperature drops to the preset value, the cooling is stopped, the temperature sensor 20 keeps real-time monitoring of the temperature inside the tub 1 during centrifugation to adjust the temperature, typically to about 37 °. After the temperature reaches the preset value, the experimenter can set the rotating speed and time of the driving motor 11 on the control panel 7 according to the experiment requirement and start the driving motor,
in the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (6)
1. A leukemia cell separation and extraction device is characterized in that: comprises a barrel body (1), a case (2) is installed at the bottom of the barrel body (1), a grab handle (3) is installed at the bottom of the case (2), a liquid injection port (4) and a liquid discharge port (5) are respectively arranged at the upper end and the lower end of a side wall of the barrel body (1), a sealing cover (6) is arranged at the top of the barrel body (1), a control panel (7) is installed on another side wall of the barrel body (1), a discharge port (8) is arranged at the bottom of the barrel body (1), a detachable battery (10) is installed inside the grab handle (3), a driving motor (11) is installed inside the driving motor (11), a water tank (12), a secondary separation chamber (15) and a filter screen (16) are arranged inside the barrel body (1) from outside to inside, the liquid injection port (4) and the liquid discharge port (5) are respectively communicated with the water tank (12), and a semiconductor refrigeration sheet (21) is installed at, the output of driving motor (11) pass through drive shaft (17) connect in the bottom of filter screen (16), bin outlet (8) with secondary separation chamber (15) are linked together, the bottom of sealed lid (6) is connected with glass fiber column layer (19) through connecting rod (18), glass fiber column layer (19) are located the inside of filter screen (16), temperature sensor (20) are installed to the outer wall of staving (1), battery (10) are connected with control panel (7), driving motor (11), temperature sensor (20) and semiconductor refrigeration piece (21) respectively.
2. The leukemia cell separation and extraction device according to claim 1, wherein: and a heat radiation window (9) is arranged on the outer wall of the case (2).
3. The leukemia cell separation and extraction device according to claim 1, wherein: the outer wall of the grab handle (3) is provided with anti-skid grains.
4. The leukemia cell separation and extraction device according to claim 1, wherein: the both ends of sealed lid (6) be equipped with joint piece (13) that staving (1) top is connected, the bottom of sealed lid (6) is equipped with sealing washer (14), the bottom ring channel of sealing washer (14), filter screen (16) sliding connection is in the ring channel.
5. The leukemia cell separation and extraction device according to claim 1, wherein: and a pull ring is arranged at the top of the sealing cover (6).
6. The leukemia cell separation and extraction device according to claim 1, wherein: the diameter of the filter holes of the filter screen (16) is smaller than the minimum diameter of the mononuclear cells and larger than the maximum diameter of the red blood cells.
Priority Applications (1)
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CN202110429269.1A CN112899153A (en) | 2021-04-21 | 2021-04-21 | Leukemia cell separation and extraction device |
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CN202110429269.1A CN112899153A (en) | 2021-04-21 | 2021-04-21 | Leukemia cell separation and extraction device |
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CN202110429269.1A Pending CN112899153A (en) | 2021-04-21 | 2021-04-21 | Leukemia cell separation and extraction device |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113174361A (en) * | 2021-06-10 | 2021-07-27 | 南通市第一老年病医院(上海大学附属南通医院、南通市第六人民医院、南通市肺科医院) | Pathological cell separation adsorption type extraction method |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
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US20020182209A1 (en) * | 1999-11-30 | 2002-12-05 | Osamu Nakamura | Leukemic cell-adsorbing column and adsorbent material therefor |
WO2006093205A1 (en) * | 2005-03-03 | 2006-09-08 | Kaneka Corporation | Method and device for cell preparation |
US20150068959A1 (en) * | 2012-12-14 | 2015-03-12 | Chong Zheng | Centrifugal filtration device and cell separation system with the same |
CN109439526A (en) * | 2018-11-19 | 2019-03-08 | 黎庆有 | A kind of cell engineering cell high-efficient screening plant |
CN209010514U (en) * | 2018-10-23 | 2019-06-21 | 王志刚 | A kind of tumour cell separator that internal medicine uses |
CN111182951A (en) * | 2017-08-01 | 2020-05-19 | 指南针制药有限责任公司 | Filtration and chromatography pods and methods of using the same |
CN211678270U (en) * | 2020-02-25 | 2020-10-16 | 中南大学湘雅三医院 | Leukemia lymphocyte separation and extraction device |
CN211734350U (en) * | 2019-12-11 | 2020-10-23 | 河南侨创生命科技有限公司 | Peripheral blood mononuclear cell rapid separation device |
CN212476721U (en) * | 2020-06-20 | 2021-02-05 | 徐州市中心医院 | Bone marrow mononuclear cell and platelet horizontal centrifugation enrichment device for tissue engineering |
-
2021
- 2021-04-21 CN CN202110429269.1A patent/CN112899153A/en active Pending
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020182209A1 (en) * | 1999-11-30 | 2002-12-05 | Osamu Nakamura | Leukemic cell-adsorbing column and adsorbent material therefor |
WO2006093205A1 (en) * | 2005-03-03 | 2006-09-08 | Kaneka Corporation | Method and device for cell preparation |
US20150068959A1 (en) * | 2012-12-14 | 2015-03-12 | Chong Zheng | Centrifugal filtration device and cell separation system with the same |
CN111182951A (en) * | 2017-08-01 | 2020-05-19 | 指南针制药有限责任公司 | Filtration and chromatography pods and methods of using the same |
CN209010514U (en) * | 2018-10-23 | 2019-06-21 | 王志刚 | A kind of tumour cell separator that internal medicine uses |
CN109439526A (en) * | 2018-11-19 | 2019-03-08 | 黎庆有 | A kind of cell engineering cell high-efficient screening plant |
CN211734350U (en) * | 2019-12-11 | 2020-10-23 | 河南侨创生命科技有限公司 | Peripheral blood mononuclear cell rapid separation device |
CN211678270U (en) * | 2020-02-25 | 2020-10-16 | 中南大学湘雅三医院 | Leukemia lymphocyte separation and extraction device |
CN212476721U (en) * | 2020-06-20 | 2021-02-05 | 徐州市中心医院 | Bone marrow mononuclear cell and platelet horizontal centrifugation enrichment device for tissue engineering |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113174361A (en) * | 2021-06-10 | 2021-07-27 | 南通市第一老年病医院(上海大学附属南通医院、南通市第六人民医院、南通市肺科医院) | Pathological cell separation adsorption type extraction method |
CN113174361B (en) * | 2021-06-10 | 2023-11-14 | 南通市第一老年病医院(上海大学附属南通医院、南通市第六人民医院、南通市肺科医院) | Pathological cell separation adsorption type extraction method |
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Application publication date: 20210604 |