CN112871000A

CN112871000A - Dye mixed liquid configuration method and computer storage medium

Info

Publication number: CN112871000A
Application number: CN201911212867.2A
Authority: CN
Inventors: 刘隐明; 滕锦
Original assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Current assignee: Shenzhen Mindray Bio Medical Electronics Co Ltd
Priority date: 2019-11-29
Filing date: 2019-11-29
Publication date: 2021-06-01

Abstract

The invention provides a preparation method of a dye mixed solution, which is applied to a push piece dyeing machine, wherein the push piece dyeing machine comprises a quantitative pump assembly, and the method comprises the following steps: determining the target volume ratio of a dye solution and a buffer solution in a target dye mixed solution; controlling the constant delivery pump assembly to respectively suck dye liquor and buffer liquor; determining target times respectively corresponding to the dye liquor and the buffer liquor output by the quantitative pump assembly according to the target volume proportion; controlling the quantitative pump assembly to stop discharging liquor after the times of outputting the dye liquor and the buffer liquor are respectively corresponding target times; and mixing the dye solution and the buffer solution to form the target dye mixed solution. The invention also provides a computer storage medium for executing the configuration method of the dye mixed liquor. The preparation method of the dye mixed liquid provided by the invention can increase the preparation concentration types of the dye mixed liquid.

Description

Dye mixed liquid configuration method and computer storage medium

Technical Field

The invention relates to the field of medical equipment, in particular to a preparation method of dye mixed liquid and a computer storage medium.

Background

In the application of the slide dyeing machine to the dyeing analysis of biological tissues, the dyeing of different chroma can be realized on the sample through dye mixed liquor with different concentrations. And because the liquid outlet range of the dye liquor and the buffer liquor output by the quantitative pump is fixed, the concentration types of the prepared dye mixed liquor of the dye liquor and the buffer liquor are very few, and the requirement of configuring the dye mixed liquor with various percentage concentrations by a user cannot be met. Therefore, how to increase the concentration types of the dye mixture solution, the dye mixture solution with more concentration is a technical problem to be solved.

Disclosure of Invention

The invention provides a method for preparing a dye mixed solution and a computer storage medium, which can increase the preparation concentration types of the dye mixed solution.

In a first aspect, the present invention provides a method for preparing a dye mixture, which is applied to a push-piece dyeing machine, where the push-piece dyeing machine includes a dosing pump assembly, and the method includes:

determining the target volume ratio of a dye solution and a buffer solution in a target dye mixed solution;

controlling the constant delivery pump assembly to respectively suck dye liquor and buffer liquor;

determining target times respectively corresponding to the dye liquor and the buffer liquor output by the quantitative pump assembly according to the target volume proportion;

controlling the quantitative pump assembly to stop discharging liquor after the times of outputting the dye liquor and the buffer liquor are respectively corresponding target times;

and mixing the dye solution and the buffer solution to form the target dye mixed solution.

In a second aspect, the present invention provides a method for preparing a dye mixture, which is applied to a push-piece dyeing machine, where the push-piece dyeing machine includes a dosing pump assembly, and the method includes:

determining the target concentration of the target dye mixed liquor;

determining target times respectively corresponding to the dye liquor and the buffer liquor output by the quantitative pump assembly according to the target concentration;

In a third aspect, the present invention provides a computer storage medium storing a computer program comprising program instructions that, when executed by a processor, perform the method according to any one of the above embodiments.

According to the embodiment of the invention, the target volume proportion of the target dye mixed liquor is determined, the quantitative pump assembly is controlled to respectively suck the dye liquor and the buffer liquor according to the target dye mixed liquor, the target times of the quantitative pump assembly for outputting the dye liquor and the buffer liquor respectively are determined according to the target volume proportion, the quantitative pump assembly is controlled to stop discharging liquor after the times of outputting the dye liquor and the buffer liquor are respectively the corresponding target times, and the dye liquor and the buffer liquor are mixed to form the target dye mixed liquor, so that the concentration types of the dye mixed liquor configured by the push sheet dyeing machine are increased, the dye mixed liquor with various concentrations is configured, the difference of the dyeing depth of a cell sample is realized, and different clinical requirements are met.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.

Fig. 1 is a flowchart of a method for preparing a dye mixture according to an embodiment of the present invention;

FIG. 2 is a flow chart of a method for preparing a dye mixture according to a second embodiment of the present invention;

fig. 3 is a schematic structural diagram of a push piece dyeing machine according to a second embodiment of the present invention;

fig. 4 is a schematic structural diagram of another push piece dyeing machine provided in the second embodiment of the present invention;

FIG. 5 is a flow chart of a method for preparing a dye mixture according to a third embodiment of the present invention;

fig. 6 is a schematic structural diagram of a push piece dyeing machine according to a third embodiment of the present invention;

fig. 7 is a flowchart of a method for configuring a dye mixture according to a fourth embodiment of the present invention.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The embodiments of the present invention can be combined with each other as appropriate.

The slide-pushing dyeing machine is used for pushing a cell sample in biological tissues or blood on a glass slide, spreading the cell sample on the glass slide, and then dyeing the cell sample, so that the detailed structure of the cell sample can be observed more clearly under a microscope. The dye mixture for staining the cell sample generally includes a staining solution capable of staining the cell sample and a buffer solution for diluting the staining solution.

In the dyeing step, a quantitative pump assembly is adopted to add a dye solution and a buffer solution into a container (also called a dyeing box) filled with a cell sample, and because the liquid outlet ranges of the quantitative pump filled with the dye solution and the quantitative pump filled with the buffer solution are fixed, the volumes of the dye solution and the buffer solution output into the container filled with the cell sample by the quantitative pump assembly are fixed. Therefore, only a fixed ratio of mixed liquor can be formed by the fixed displacement pump assembly. For example, the push piece dyeing machine comprises two fixed pumps, wherein one fixed pump is filled with dye liquor, and the liquor discharging range of the fixed pump is 0.6 ml. The other quantitative pump is filled with buffer solution, and the liquid output range of the quantitative pump is 5.4 ml. The concentration of the dye mixture solution thus obtained was 10%. The dye mixed solution with fixed concentration can not meet the requirement of carrying out various staining on the cell sample.

If dye mixed liquid with more concentration proportion is obtained, the push piece dyeing machine needs to be provided with a plurality of quantitative pumps with different liquid outlet measuring ranges, so that the structure of the push piece dyeing machine is complex, the cost is high, the volume is large, and the structural improvement and the cost reduction of the push piece dyeing machine are not facilitated.

As shown in fig. 1, a method for preparing a dye mixture is provided in an embodiment of the present invention, and is applied to a slide-dyeing machine, a cell-dyeing machine, and the like. The present invention will be described by taking an example in which the method for preparing a dye mixture is applied to a slide dyeing machine. The push piece dyeing machine comprises a quantitative pump assembly. The method for preparing the dye mixed liquid provided by the embodiment increases the concentration types of the dye mixed liquid prepared by the push piece dyeing machine so as to prepare the dye mixed liquid with various concentrations, realizes the difference of dyeing depth of the cell sample, and meets different clinical requirements. The above configuration method includes the following steps.

110: and determining the target volume ratio of the dye solution and the buffer solution in the target dye mixed solution.

In one embodiment, in the slide dyeing machine, a plurality of volume ratios of the dye solution and the buffer solution in the target dye mixture may be set in advance. It will be appreciated that the dye and buffer may be pre-set. The type of the dye solution can be one or more, and the type of the buffer solution can be one or more. The volume ratio of dye to buffer includes, but is not limited to, 1:18, 1:9, 1:6, 2:9, etc. When the types of the dye liquors are two, the first dye liquor is: a second dye solution: the volume ratio of the buffer includes, but is not limited to, 1:1:18, 1:2:18, and the like.

The operator selects the target volume proportion of the dye solution and the buffer solution which are required to be configured currently from the preset multiple volume proportions. For example, a target volume ratio of dye to buffer to be currently dispensed is selected to be 1: 9.

In another embodiment, the operator inputs the target volume ratio of the dye liquor and the buffer liquor to be configured currently in the push-piece dyeing machine, for example, the target volume ratio of the dye liquor to be configured currently is 1: 9.

120: and controlling a quantitative pump assembly to respectively suck the dye liquor and the buffer liquor.

Specifically, the push piece dyeing machine comprises a quantitative pump assembly, wherein the quantitative pump assembly comprises at least one liquid inlet, at least one quantitative pump and at least one liquid outlet. Each quantitative pump is communicated with at least one liquid inlet and forms a liquid inlet passage. Each fixed displacement pump is communicated with one liquid outlet and forms a liquid outlet passage. One liquid inlet is communicated with a storage container for storing dye liquor or buffer solution. The liquid outlet is communicated with a container filled with a cell sample.

In one embodiment, the target dye mixture may be a plurality of types, for example, the combination of the target dye mixture may be a first dye solution and a first buffer solution, a second dye solution and a second buffer solution, a first dye solution and a second buffer solution, a second dye solution and a first buffer solution, and so on. Wherein the first dye liquor and the second dye liquor are different kinds of dye liquor. The first buffer solution and the second buffer solution are the same or different buffer solutions.

And determining the type of the dye liquor and the type of the buffer liquor according to the target dye mixed liquor input or selected by an operator in the push-piece dyeing machine. For example, a first dye solution and a first buffer solution.

In one embodiment, the target dye mixture is preset as a first dye solution + a first buffer solution, and the quantitative pump assembly is controlled to respectively absorb the first dye solution and the first buffer solution. Wherein, first dye liquor and first buffer solution can be drawn and output by same dosing pump, and is specific, and a dosing pump in the dosing pump subassembly draws many times and discharges first dye liquor many times, and this dosing pump still draws many times and discharges first buffer solution. The first dye solution and the first buffer solution can be sucked and output by different quantitative pumps, and specifically, the first dye solution and the first buffer solution can be respectively sucked and output by two different quantitative pumps. The first dye solution can be sucked and output by two or more quantitative pumps, and the first buffer solution can also be sucked and output by two or more quantitative pumps.

It can be understood that the process of the dosing pump sucking up the dye liquor is to suck up the dye liquor in the storage container storing the dye liquor through the liquor inlet passage until the liquor chamber of the dosing pump is filled up. The process of the quantitative pump absorbing the buffer solution is to absorb the buffer solution in a storage container storing the buffer solution through a liquid inlet passage until a liquid cavity of the quantitative pump is filled.

It should be understood that the order between the steps in this application is not specifically limited, that is, the order between the steps in the embodiment of this application may be exchanged, and the embodiments after the exchange are also within the protection scope of this application.

130: and determining the target times respectively corresponding to the dye liquor and the buffer liquor output by the quantitative pump assembly according to the target volume proportion.

Specifically, the liquor discharging range of the quantitative pump for sucking the dye liquor and the liquor discharging range of the quantitative pump for sucking the buffer liquor are determined, a mapping relation between target volume ratios of the dye liquor and the buffer liquor and target times corresponding to the dye liquor and the buffer liquor output by the quantitative pump assembly is established according to the liquor discharging range of the quantitative pump for sucking the dye liquor and the liquor discharging range of the quantitative pump for sucking the buffer liquor, and the target times corresponding to the dye liquor and the buffer liquor output by the quantitative pump assembly are controlled according to the mapping relation.

In this embodiment, a quantitative pump with a liquid discharge range of 0.3ml is used to suck the dye solution, and a quantitative pump with a liquid discharge range of 5.4ml is used to suck the buffer solution for illustration, which will not be described again in the following. The mapping relation is that the target volume ratio of the dye solution to the buffer solution in the target dye mixed solution is V1: v2 ═ 0.3 × n: (0.3 × n +5.4 × m), where n is the target number of times that the dosing pump assembly outputs the dye solution, and m is the target number of times that the dosing pump assembly outputs the buffer solution. And determining a plurality of possible values of n and a plurality of possible values of m according to the mapping relation, the target volume proportion of the dye liquor and the buffer liquor. In one embodiment, in the case that the total volume (0.3 × n +5.4 × m) of the target dye mixture is equal to or greater than the preset volume, the minimum value is taken from the plurality of possible values of n and the minimum value is taken from the plurality of possible values of m, so as to save the usage amount of the dye solution and the buffer solution on the basis of ensuring the dyeing effect on the cell sample. In this embodiment, a minimum value is taken from a plurality of available values of n and a minimum value is taken from a plurality of available values of m for example when the total volume of the target dye mixed solution is greater than or equal to 5ml, and details are not repeated in the following. Of course, the preset volume is not limited in particular, and in other embodiments, the preset volume may be 6ml, 7ml, or 10ml, etc.

When the target volume ratio of the dye liquor to the buffer liquor is 1:9, the target times corresponding to the dye liquor and the buffer liquor can be determined to be 2 times and 1 time respectively. When the target volume ratio of the target dye mixed liquor is 1:18, the target times corresponding to the dye liquor and the buffer liquor can be determined to be 1 time and 1 time respectively. When the target volume ratio of the target dye mixed liquor is 1:6, the target times corresponding to the dye liquor and the buffer liquor can be determined to be 3 times and 1 time respectively. When the target volume ratio of the target dye mixed liquor is 2:9, the target times corresponding to the dye liquor and the buffer liquor can be determined to be 4 times and 1 time respectively.

Of course, in other embodiments, n may not take the minimum value among a plurality of possible values, and m may not take the minimum value among a plurality of possible values. For example, when the target volume ratio of the dye liquor and the buffer liquor is 1:9, the target times for the dye liquor and the buffer liquor are 4 times and 2 times, respectively, or the target times for the dye liquor and the buffer liquor are 8 times and 4 times, respectively, and so on. The same is true for other target volume ratios of dye liquor and buffer liquor, which are not further enumerated here.

140: and controlling the quantitative pump assembly to stop discharging the liquor after the times of outputting the dye liquor and the buffer liquor are respectively the corresponding target times.

Specifically, the times of controlling the quantitative pump assembly to output the dye solution and the buffer solution are respectively the target times corresponding to the dye solution and the buffer solution. For example, according to step 130, the target times corresponding to the dye solution and the buffer solution are determined to be 2 times and 1 time, and the discharging of the dye solution is stopped after the quantitative pump assembly is controlled to output the dye solution and the buffer solution for 2 times and 1 time, respectively.

It can be understood that the process of one-time dye liquor output by the quantitative pump is that the dye liquor in the liquor chamber of the quantitative pump is completely output to the container filled with the cell sample through the liquor outlet passage. The process of outputting the buffer solution by the quantitative pump at one time is to output the buffer solution in the liquid chamber of the quantitative pump to the container filled with the cell sample through the liquid outlet passage.

150: and mixing the dye solution and the buffer solution to form a target dye mixed solution.

Specifically, the dye solution output by the corresponding target times and the buffer solution output by the corresponding target times are mixed in a container filled with the cell sample to form a target dye mixed solution.

For example, the target volume ratio of the dye solution to the buffer solution is 1:9, the target times corresponding to the dye solution and the buffer solution are determined to be 2 times and 1 time respectively according to the step 130, the dye solution and the buffer solution are stopped to discharge after the times of outputting the dye solution and the buffer solution by the quantitative pump assembly are 2 times and 1 time respectively, and the dye solution output for 2 times and the buffer solution output for 1 time are mixed in a container filled with the cell sample to form the target dye mixed solution with the target volume ratio of the dye solution to the buffer solution being 1: 9.

According to the embodiment provided by the invention, the target volume proportion of the dye solution and the buffer solution is determined, the quantitative pump assembly is controlled to respectively suck the dye solution and the buffer solution according to the target dye mixed solution, the target times respectively corresponding to the dye solution and the buffer solution output by the quantitative pump assembly are determined according to the target volume proportion, the quantitative pump assembly is controlled to stop discharging the dye solution after the times of outputting the dye solution and the buffer solution are respectively the corresponding target times, and the dye solution and the buffer solution are mixed to form the target dye mixed solution.

As shown in fig. 2, a second embodiment of the present invention provides a method for preparing a dye mixture, which is applied to a slide-dyeing machine, a cell-dyeing machine, and the like. The present invention will be described by taking an example in which the method for preparing a dye mixture is applied to a slide dyeing machine. As shown in fig. 3, the push tab dying machine 100 includes a dosing pump assembly 10. The method for preparing the dye mixed liquid provided by the embodiment increases the concentration types of the dye mixed liquid prepared by the push piece dyeing machine 100 so as to prepare the dye mixed liquid with various concentrations, thereby realizing the difference of dyeing depth of the cell sample and meeting different clinical requirements. The above configuration method includes the following steps.

210: and determining the target volume ratio of the dye solution and the buffer solution in the target dye mixed solution.

The content of step 110 may be referred to in this step, and is not described herein again.

220: as shown in FIG. 3, the first quantitative pump 1 and the second quantitative pump 2 are controlled to suck the dye solution, and the third quantitative pump 3 is controlled to suck the buffer solution.

Specifically, as shown in fig. 3, the fixed displacement pump assembly 10 includes a first fixed displacement pump 1, a second fixed displacement pump 2, and a third fixed displacement pump 3. The first quantitative pump 1 is connected to a storage container for storing a staining solution through a first liquid inlet 11, and is connected to a container 20 for containing a cell sample through a first liquid outlet 12. The second quantitative pump 2 is connected to a storage container for storing a staining solution through a second liquid inlet 21, and is connected to a container 20 for containing a cell sample through a second liquid outlet 22. The third quantitative pump 3 is connected to a storage container for storing a buffer solution through a third liquid inlet 31, and is connected to the container 20 containing the cell sample through a third liquid outlet 32. The container 20 containing the cell sample is placed in the mixed solution staining station.

As shown in fig. 3, a two-position three-way valve 13 is disposed between the first fixed displacement pump 1 and the first liquid inlet 11, and the two-position three-way valve 13 is further disposed between the first fixed displacement pump 1 and the first liquid outlet 12. The first dosing pump 1 comprises a gas chamber 14, a liquid chamber 15 and an elastic diaphragm 16 separating the gas chamber 14 from the liquid chamber 15. The dosing pump assembly 10 further comprises a first air valve 17 which controls the air pressure within the air chamber 14. The gas in the gas chamber 14 is caused to exert a positive or negative pressure on the liquid chamber 15 of the first volumetric pump 1 by controlling the first gas valve 17 to inflate or deflate the gas chamber 14.

In the process of sucking the dye liquor by the first quantitative pump 1, communicating the first liquid inlet 11 with the liquid chamber 15 of the first quantitative pump 1 by controlling the two-position three-way valve 13 (at the moment, the first liquid outlet 12 is disconnected with the liquid chamber 15 of the first quantitative pump 1), and controlling the first air valve 17 to apply negative pressure to the air chamber 14 so as to enable the liquid chamber 15 of the first quantitative pump 1 to be full of the dye liquor; in the process of outputting the dye liquor by the first quantitative pump 1, the first liquid outlet 12 is communicated with the liquid chamber 15 of the first quantitative pump 1 by controlling the two-position three-way valve 13 (at this time, the first liquid inlet 11 is disconnected from the liquid chamber 15 of the first quantitative pump 1), and the first air valve 17 is controlled to make the air pressure in the air chamber 14 reach the preset air pressure (the preset air pressure is positive pressure and is greater than the hydraulic pressure in the liquid chamber 15), so that the dye liquor in the liquid chamber 15 of the first quantitative pump 1 is output through the first liquid outlet 12.

In other embodiments, the two-position three-way valve 13 may be replaced by two-way valves. The two-way valves are respectively arranged between the first fixed displacement pump 1 and the first liquid inlet 11 and between the first fixed displacement pump 1 and the first liquid outlet 12.

In other embodiments, the first air valve 17 may be replaced by a solenoid valve, and the solenoid valve is controlled to push the elastic diaphragm 16 to apply positive or negative pressure to the liquid chamber 15 of the fixed displacement pump by opening the solenoid valve.

The second fixed displacement pump 2 and the third fixed displacement pump 3 have the same structure as the first fixed displacement pump 1, and are not described again. A two-position three-way valve 23 is arranged between the second quantitative pump 2 and the second liquid inlet 21, and the two-position three-way valve 23 is also arranged between the second quantitative pump 2 and the second liquid outlet 22. The valve controlling the air pressure in the air chamber of the second fixed displacement pump 2 is a second air valve 24. A first liquid valve 33 is arranged between the third fixed displacement pump 3 and the third liquid inlet 31, and a second liquid valve 34 is arranged between the third fixed displacement pump 3 and the second liquid outlet 22. The valve controlling the air pressure in the air chamber of the third fixed displacement pump 3 is a third air valve 35.

The process of sucking or outputting the dye liquor by the second quantitative pump 2 can refer to the process of sucking or outputting the dye liquor by the first quantitative pump 1, and the process of sucking or outputting the buffer solution by the third quantitative pump 3 can refer to the process of sucking or outputting the dye liquor by the first quantitative pump 1.

In the above embodiment, the first quantitative pump 1 and the second quantitative pump 2 both suck the dye solution, and the third quantitative pump 3 sucks the buffer solution. In other embodiments, the dye liquor may be drawn by a dosing pump. As shown in fig. 4, the fixed displacement pump assembly 10 includes a first fixed displacement pump 1 and a second fixed displacement pump 2. The first quantitative pump 1 is connected to a storage container for storing a staining solution through a first liquid inlet 11, and is connected to a container 20 for containing a cell sample through a first liquid outlet 12. The second quantitative pump 2 is connected to a storage container for storing a buffer solution through a second liquid inlet 21, and is connected to a container 20 for containing a cell sample through a second liquid outlet 22. Compared with the constant delivery pump assembly 10 in the second embodiment, the constant delivery pump assembly 10 in the present embodiment has one less constant delivery pump, so that the overall structure of the push piece dyeing machine 100 is simplified and the cost is saved.

230: and determining a first target number of times of outputting the dye liquor and a second target number of times of outputting the buffer liquor by the quantitative pump assembly 10 according to the target volume proportion, the volume of the liquor chamber for storing the dye liquor and the volume of the liquor chamber for storing the buffer liquor.

Specifically, the steps of determining a first target number of times that the fixed displacement pump assembly 10 outputs the dye solution and a second target number of times that the fixed displacement pump assembly outputs the buffer solution according to the target volume ratio, the volume of the solution chamber storing the dye solution, and the volume of the solution chamber storing the buffer solution include:

and determining a first target volume of the dye liquor output by the quantitative pump assembly 10 and a second target volume of the buffer liquor according to the target volume proportion, the volume of the liquor chamber for storing the dye liquor and the volume of the liquor chamber for storing the buffer liquor. Wherein, the ratio of the first target volume of the dye solution to the second target volume of the buffer solution is a target volume ratio. The first target volume is a first multiple of a volume of a liquor chamber storing the liquor, the first multiple being an integer greater than or equal to 0. The second target volume is a second multiplier of the volume of the liquid chamber storing the buffer, the second multiplier is an integer greater than or equal to 0, and the sum of the first multiplier and the second multiplier is an integer greater than or equal to 1. The first multiplier value is determined to be a first target number of times that the dosing pump assembly 10 outputs the dye liquor. The second multiplier value is determined to be a second target number of times the dosing pump assembly 10 outputs buffer.

In this embodiment, the liquid discharging ranges of the first quantitative pump 1 and the second quantitative pump 2 are 0.3ml, the liquid discharging range of the third quantitative pump 3 is 5.4ml, the first quantitative pump 1 and the second quantitative pump 2 are used for outputting a dye solution, and the third quantitative pump 3 is used for outputting a buffer solution, and under the condition that the total volume of the target dye mixed solution is ensured to be greater than 5ml, the smallest first target volume and the smallest second target volume among a plurality of available values are exemplified according to a mapping relationship, and will not be described again in the following.

For example, the target volume ratio of the dye solution to the buffer solution to be prepared is 1:9, the volume of the dye solution output by the quantitative pump assembly 10 can be obtained as 0.6ml in the first target volume which is the smallest among the plurality of possible values, and the volume of the buffer solution output by the quantitative pump assembly 10 is 5.4ml in the second target volume which is the smallest among the plurality of possible values. When the liquid discharging range of the quantitative pump for outputting the dye liquid is 0.3ml and the liquid discharging range of the quantitative pump for outputting the buffer liquid is 5.4ml, it can be determined that the first target number of times that the quantitative pump assembly 10 outputs the dye liquid is 2 times and the second target number of times that the quantitative pump assembly 10 outputs the buffer liquid is 1 time. In one case, the first quantitative pump 1 and the second quantitative pump 2 output the dye solution, the number of times that the first quantitative pump 1 and the second quantitative pump 2 output the dye solution may be determined to be 1, respectively, and the number of times that the third quantitative pump 3 outputs the buffer solution may be determined to be 1. In another case, the first quantitative pump 1 outputs the dye solution and the second quantitative pump 2 does not output the dye solution, and it can be determined that the first quantitative pump 1 outputs the dye solution 2 times, the second quantitative pump 2 outputs the dye solution 0 times, and the third quantitative pump 3 outputs the buffer solution 1 times.

For example, the target volume ratio of the dye solution to the buffer solution to be prepared is 1:18, the volume of the dye solution output by the quantitative pump assembly 10 can be obtained as 0.3ml in the first target volume which is the smallest among the plurality of possible values, and the volume of the buffer solution output by the quantitative pump assembly 10 is 5.4ml in the second target volume which is the smallest among the plurality of possible values. When the liquid discharging range of the quantitative pump for outputting the dye liquid is 0.3ml and the liquid discharging range of the quantitative pump for outputting the buffer liquid is 5.4ml, the first target times of outputting the dye liquid by the quantitative pump assembly 10 can be determined to be 1 time, and the second target times of outputting the buffer liquid by the quantitative pump assembly 10 can be determined to be 1 time. In one case, the first constant delivery pump 1 delivers the dye solution and the second constant delivery pump 2 does not deliver the dye solution, and it can be determined that the first constant delivery pump 1 delivers the dye solution 1 times, the second constant delivery pump 2 delivers the dye solution 0 times, and the third constant delivery pump 3 delivers the buffer solution 1 times. In another case, the second quantitative pump 2 outputs the dye solution and the first quantitative pump 1 does not output the dye solution, and it can be determined that the number of times that the first quantitative pump 1 outputs the dye solution is 0, the number of times that the second quantitative pump 2 outputs the dye solution is 1, and the number of times that the third quantitative pump 3 outputs the buffer solution is 1.

For example, the target volume ratio of the dye solution to the buffer solution to be prepared is 1:6, the volume of the dye solution output by the quantitative pump assembly 10 can be obtained as 0.9ml in the first target volume which is the smallest among the plurality of possible values, and the volume of the buffer solution output by the quantitative pump assembly 10 is 5.4ml in the second target volume which is the smallest among the plurality of possible values. When the liquid discharging range of the quantitative pump for outputting the dye liquid is 0.3ml and the liquid discharging range of the quantitative pump for outputting the buffer liquid is 5.4ml, it can be determined that the first target number of times that the quantitative pump assembly 10 outputs the dye liquid is 3 times and the second target number of times that the quantitative pump assembly 10 outputs the buffer liquid is 1 time. The mode that the quantitative pump assembly 10 outputs the dye liquor for 3 times comprises the following steps: the first fixed displacement pump 1 outputs 0 times, and the second fixed displacement pump 2 outputs 3 times; or the first fixed displacement pump 1 outputs 1 time, and the second fixed displacement pump 2 outputs 2 times; or the first fixed displacement pump 1 outputs 2 times, and the second fixed displacement pump 2 outputs 1 time; alternatively, the first fixed displacement pump 1 outputs 3 times, and the second fixed displacement pump 2 outputs 0 time.

For example, the target volume ratio of the dye solution to the buffer solution to be prepared is 2:9, the volume of the dye solution output by the quantitative pump assembly 10 can be 1.2ml by the smallest first target volume among the plurality of values, and the volume of the buffer solution output by the quantitative pump assembly 10 is 5.4ml by the smallest second target volume among the plurality of values. When the liquid discharging range of the quantitative pump for outputting the dye liquid is 0.3ml and the liquid discharging range of the quantitative pump for outputting the buffer liquid is 5.4ml, it can be determined that the first target number of times that the quantitative pump assembly 10 outputs the dye liquid is 4 times and the second target number of times that the quantitative pump assembly 10 outputs the buffer liquid is 1 time. The way that the quantitative pump assembly 10 outputs the dye liquor 4 times includes: the first fixed displacement pump 1 outputs 0 times, and the second fixed displacement pump 2 outputs 4 times; or the first fixed displacement pump 1 outputs 1 time, and the second fixed displacement pump 2 outputs 3 times; or the first fixed displacement pump 1 outputs 2 times, and the second fixed displacement pump 2 outputs 2 times; or the first fixed displacement pump 1 outputs 3 times, and the second fixed displacement pump 2 outputs 1 time; alternatively, the first fixed displacement pump 1 outputs 4 times, and the second fixed displacement pump 2 outputs 0 time.

In addition, the target volume ratio of the dye solution to be prepared to the buffer solution can be 0:5.4, the volume of the dye solution output by the quantitative pump assembly 10 can be 0ml by the smallest first target volume in a plurality of available values, and the volume of the buffer solution output by the quantitative pump assembly 10 is 5.4ml by the smallest second target volume in the plurality of available values. It can be determined that the first target number of times that the quantitative pump assembly 10 outputs the dye solution is 0 times and the second target number of times that the quantitative pump assembly 10 outputs the buffer solution is 1 time. Since a small amount of dye solution remains in the dyeing box during the last dyeing process, the dyeing can be performed without adding the dye solution in the embodiment.

It can be understood that the liquid discharge method of the present embodiment may also be used to form a target dye mixture solution with other concentration. The concentration of the mixed solution of the target dye is not particularly limited in the present invention.

It can be understood that the output ranges of the first quantitative pump 1 and the second quantitative pump 2 can be different, so as to combine the target dye mixed liquor in more proportion. The ranges of the discharge of the first quantitative pump 1 and the second quantitative pump 2 are not limited to 0.3ml, and may be 0.4ml, 0.2ml, 0.5ml, or the like. The range of the third quantitative pump 3 is not limited to 5.4ml, and may be 2.7ml, 5.2ml, 5.6ml, 5.0ml, or the like. Thus, target dye mixed liquor with more concentration types is realized.

It can be understood that, when the target volume ratio of the dye solution to the buffer solution to be prepared is 1:9, the present invention does not limit the volume of the dye solution output by the quantitative pump assembly 10 to be 0.6ml and the volume of the buffer solution output by the quantitative pump assembly 10 to be 5.4ml, and may also limit the volume of the dye solution output by the quantitative pump assembly 10 to be 1.2ml and the volume of the buffer solution output by the quantitative pump assembly 10 to be 10.8ml, etc. Similarly, the same applies to other ratios, and the total volume of the dye mixture is not particularly limited in the present invention.

240: and controlling the quantitative pump assembly 10 to stop discharging the liquor after the times of outputting the dye liquor and the buffer liquor are respectively the corresponding target times.

In the first embodiment, when the second quantitative pump 2 adds the buffer solution with the first volume into the container, the first quantitative pump 1 is controlled to stop the liquor discharging after the dye solution with the corresponding target times is added into the container, and the second quantitative pump 2 is controlled to stop the liquor discharging after the buffer solution with the second volume is added into the container, wherein the sum of the first volume and the second volume is the sum of the volumes of the buffer solution with the corresponding target times output by the second quantitative pump 2.

For example, when the first quantitative pump 1 and the second quantitative pump 2 need to output 0.3ml of the dye solution and the third quantitative pump 3 needs to output 5.4ml of the buffer solution, the third quantitative pump 3 is controlled to output the buffer solution into the container 20 containing the cell sample. When the output buffer solution is about 1ml, the first quantitative pump 1 and the second quantitative pump 2 are controlled to simultaneously output 0.3ml of the dye solution into the container 20 filled with the cell sample, and after the dye solution is completely output, the buffer solution is also completely output, so that the dye solution and the buffer solution can be uniformly mixed in the container 20 filled with the cell sample. Certainly, in this embodiment, the output of the dye solution is not limited to be started when the buffer solution is output by about 1ml, and the dye solution can also be started to be output when the buffer solution is output by about 2ml or 3ml, which will not be described in detail later.

For another example, when the first quantitative pump 1 needs to output 0.3ml of the dye solution and the third quantitative pump 3 needs to output 5.4ml of the buffer solution, the third quantitative pump 3 outputs the buffer solution into the container 20 containing the cell sample, when the volume of the buffer solution is about 1ml, the first quantitative pump 1 outputs 0.3ml of the dye solution into the container 20 containing the cell sample, and after the dye solution is output, the buffer solution is discharged.

By adding the dye solution in the process of discharging the buffer solution into the dyeing box, the buffer solution and the dye solution can be uniformly mixed when discharging the buffer solution into the dyeing box.

In one embodiment, the control flow of the liquid output of the first quantitative pump 1 is as follows: after the liquid valve (two-position three-way valve 13) is opened, the first air valve 17 is opened to increase the air pressure in the air chamber 14 to a predetermined air pressure, for example, 70 Kpa. At this time, the air pressure in the air chamber 14 is greater than the hydraulic pressure in the liquid chamber 15, so that the dye liquid in the liquid chamber 15 is output through the first liquid outlet 12. After the liquid outlet is finished, the liquid valve is closed, and then the first air valve 17 is closed. Of course, the liquid valve and the first gas valve 17 may be opened and closed at the same time.

In other embodiments, the control flow of the liquid output of the first quantitative pump 1 is as follows: after the liquid valve is opened, the electromagnetic valve is opened to enable the electromagnetic valve to push the elastic diaphragm, so that the pressure of the electromagnetic valve on the elastic diaphragm 16 is greater than the hydraulic pressure in the liquid chamber 15, and the dye liquid in the liquid chamber 15 is output through the first liquid outlet 12. And after liquid outlet is finished, closing the liquid valve and then closing the electromagnetic valve. Of course, the liquid valve and the solenoid valve may be opened and closed simultaneously.

In a second embodiment, the method for controlling the quantitative pump assembly 10 to stop discharging the dye solution after the dye solution and the buffer solution are respectively discharged for the corresponding target times comprises the following steps:

in the process of controlling the second quantitative pump 2 to add the buffer solution with the first volume into the container, the first quantitative pump 1 is controlled to stop liquor discharging after the dye liquor with the first time is added into the container.

And in the process of controlling the second quantitative pump 2 to add a second volume of buffer solution into the container, controlling the first quantitative pump 1 to stop liquor discharging after adding the dye liquor into the container for a second time, wherein the sum of the first time and the second time is the target time corresponding to the dye liquor output by the first quantitative pump 1, and the sum of the first volume and the second volume is the sum of the volumes of the buffer solution output by the second quantitative pump 2 for the target time.

For example, when the first quantitative pump 1 needs to output 0.6ml of the dye solution, the second quantitative pump 2 needs to output 0.3ml of the dye solution, and the third quantitative pump 3 needs to output 5.4ml of the buffer solution, the third quantitative pump 3 divides the buffer solution into two times (the second target number is still 1 time according to the buffer solution that has been completely output, which will not be described in detail later), the amount of the buffer solution that is output by the third quantitative pump 3 to the container 20 containing the cell sample for the first time is about 2.7ml by controlling the liquid output time of the third quantitative pump 3, and the first quantitative pump 1 outputs 0.3ml of the dye solution to the container 20 containing the cell sample during the first liquid output process of the buffer solution. After the first time of the buffer solution and the dye solution are both completely delivered, the buffer solution is delivered for the second time, 2.7ml of the buffer solution left in the solution chamber of the third quantitative pump 3 is completely delivered, and 0.3ml of the dye solution is delivered into the container 20 filled with the cell sample by the first quantitative pump 1 and the second quantitative pump 2 simultaneously in the process of delivering the buffer solution for the second time. The method for outputting the buffer solution of the liquid chamber of the third constant delivery pump 3 in two times comprises the steps of controlling the liquid valve of the third constant delivery pump to open firstly, then opening the air valve of the third constant delivery pump, closing the liquid valve when the liquid output volume is about 2.7ml, controlling the liquid valve to open secondly, closing the liquid valve after the residual liquid volume in the liquid chamber of the third constant delivery pump 3 is completely output, and finally closing the air valve of the third constant delivery pump. The liquid valve of the third fixed displacement pump and the gas valve of the third fixed displacement pump may be operated simultaneously.

For example, when the first quantitative pump 1 needs to output 0.6ml of the dye solution, the second quantitative pump 2 needs to output 0.6ml of the dye solution, and the third quantitative pump 3 needs to output 5.4ml of the buffer solution, the third quantitative pump 3 outputs the buffer solution for the first time, the amount of the buffer solution for the first time is about 2.7ml by controlling the liquid output time of the third quantitative pump 3, and the first quantitative pump 1 and the second quantitative pump 2 are controlled to output 0.3ml of the dye solution into the container 20 containing the cell sample during the first liquid output process of the buffer solution. And after the first time of the dye solution and the buffer solution are completely output, outputting the buffer solution for the second time, completely outputting 2.7ml of the buffer solution left in the liquid chamber of the third quantitative pump 3, and controlling the first quantitative pump 1 and the second quantitative pump 2 to simultaneously output 0.3ml of the dye solution into the container 20 filled with the cell sample in the process of outputting the buffer solution for the second time.

By adding the staining solution during the process of draining the buffer solution into the container 20 containing the cell sample, it can be ensured that the buffer solution and the staining solution can be uniformly mixed when draining into the staining box.

In a third embodiment, the step of controlling the quantitative pump assembly 10 to stop discharging the dye solution after the number of times of discharging the dye solution and the buffer solution is the corresponding target number of times includes:

when a first quantitative pump 1 adds a first volume of dye liquor into a container, controlling a second quantitative pump 2 to stop liquor discharge after a second target number of times of buffer liquor is added into the container, and controlling a first quantitative pump 1 to stop liquor discharge after a second volume of dye liquor is added into the container, wherein the sum of the first volume and the second volume is the sum of the volumes of the dye liquor output by the first quantitative pump 1 for the corresponding target number of times.

In the embodiment, the dye solution is output firstly, and the buffer solution is output in the process of outputting the dye solution, because the volume of the buffer solution is relatively small, the liquid outlet speed of the buffer solution is larger than that of the dye solution by controlling the pressure of the air chamber in the third quantitative pump 3 to be larger than that of the air chambers of the first quantitative pump 1 and the second quantitative pump 2, so that the buffer solution and the dye solution in the dyeing box are mixed uniformly.

250: and mixing the dye solution and the buffer solution to form a target dye mixed solution.

Specifically, the first liquid outlet 12, the second liquid outlet 22 and the third liquid outlet 32 are all aligned to the container 20 (also called a staining box) containing the cell sample, and the liquid outlet time of the staining solution output from the first liquid outlet 12 and the second liquid outlet 22 and the liquid outlet time of the buffer solution output from the third liquid outlet 32 are controlled to coincide, that is, the buffer solution is also output when the staining solution is output, so that the staining solution and the buffer solution are uniformly mixed in the container 20 containing the cell sample.

The embodiment of the invention determines the target volume proportion of the dye liquor and the buffer liquor, controls the first quantitative pump 1 and the second quantitative pump 2 to absorb the dye liquor according to the target dye mixed liquor, controls the third quantitative pump 3 to absorb the buffer liquor, determines the first target times of the quantitative pump assembly 10 to output the dye liquor and the second target times of the buffer liquor according to the target volume proportion, the volume of a liquor chamber for storing the dye liquor and the volume of a liquor chamber for storing the buffer liquor, controls the quantitative pump assembly 10 to stop liquor discharge after the times of outputting the dye liquor and the buffer liquor are respectively corresponding target times, mixes the dye liquor and the buffer liquor to form the target dye mixed liquor, thus, by controlling the first target times of outputting the dye liquor by the first quantitative pump 1 and the second quantitative pump 2 and the second target times of outputting the buffer liquor by the third quantitative pump 3, mixes the dye mixed liquor with different concentrations, increases the concentration types of the dye mixed liquor configured by the slide dyeing machine 100, dye mixed liquor with various concentrations is prepared, so that differentiation of dyeing depth of cell samples is realized, and different clinical requirements are met; meanwhile, the time coincidence of the output dye liquor and the output buffer liquor is controlled to ensure that the buffer liquor and the dye liquor can be uniformly mixed when flowing out of the dyeing box.

As shown in fig. 5, a third embodiment of the present invention provides a method for preparing a dye mixture, which is applied to the push-piece dyeing machine 100, the cell dyeing machine, and the like shown in fig. 6. The present invention will be described by taking an example in which the method for preparing a dye mixture is applied to a slide dyeing machine 100. The push-piece dying machine 100 includes a dosing pump assembly 10. The method for preparing the dye mixed liquid provided by the embodiment increases the concentration types of the dye mixed liquid prepared by the push piece dyeing machine 100 so as to prepare the dye mixed liquid with various concentrations, thereby realizing the difference of dyeing depth of the cell sample and meeting different clinical requirements.

As shown in fig. 6, the structure of the constant quantity set pump assembly in this embodiment is the same as that in the second embodiment, so the structure of the constant quantity set pump assembly in this embodiment is not described again. The main difference is that the first fixed displacement pump 1 and the second fixed displacement pump 2 in the second embodiment both output the same dye solution, while the first fixed displacement pump 1 and the second fixed displacement pump 2 in the second embodiment output different kinds of dye solutions respectively.

The above configuration method includes the following steps.

310: and determining the target volume ratio of the dye liquor and the buffer liquor in the target dye liquor mixed liquor. The target dye solution mixed liquor comprises a first dye solution, a second dye solution and a buffer solution. Of course, in other embodiments, the target dye solution mixture may further include three or more dye solutions and multiple buffers, and this embodiment takes the example that the target dye solution mixture includes the first dye solution, the second dye solution and the buffer solution, and will not be described in detail later.

Specifically, the target volume ratio of the first dye solution, the second dye solution and the buffer solution may be 1:1:18, 1:0:18, 0:1:18, 0:2:18, 1:2:18, 0:0:18, 2:1:18, 2:2:18, and the like.

320: controlling the first quantitative pump 1 to suck the first dye solution, controlling the second quantitative pump 2 to suck the second dye solution, and controlling the third quantitative pump 3 to suck the buffer solution.

The content of step 220 can be referred to in this step, and is not described herein again.

330: and determining the third target times of outputting the first dye liquor, the fourth target times of outputting the second dye liquor and the second target times of outputting the buffer liquor by the quantitative pump assembly 10 according to the target volume proportion, the volume of the liquor chamber for storing the first dye liquor, the volume of the liquor chamber for storing the second dye liquor and the volume of the liquor chamber for storing the buffer liquor.

And determining a third target volume of the first dye solution, a fourth target volume of the second dye solution and a second target volume of the buffer solution output by the quantitative pump assembly 10 according to the target volume proportion, the volume of the solution chamber for storing the first dye solution, the volume of the solution chamber for storing the second dye solution and the volume of the solution chamber for storing the buffer solution. The ratio of the third target volume, the fourth target volume and the second target volume is a target volume ratio.

Wherein the third target volume is a third value of the volume of the liquor chamber storing the first dyeing liquor, the third value being an integer greater than or equal to 0. The fourth target volume is a quadruple value of the volume of the liquor chamber storing the second dyeing liquor, the quadruple value being an integer greater than or equal to 0.

The second target volume is a second multiplier of the volume of the liquid chamber storing the buffer, the second multiplier being an integer greater than or equal to 0, and the sum of the second multiplier and the third and fourth multipliers being an integer greater than or equal to 1. The second multiplier is determined to be a second target number of times the third quantitative pump 3 outputs the buffer solution. And determining the third time value as a third target number of times that the first quantitative pump 1 outputs the first dye liquor. The fourth factor value is determined as a fourth target number of times the second dosing pump 2 outputs the second dye liquor.

In this embodiment, the liquid discharging ranges of the first quantitative pump 1 and the second quantitative pump 2 are 0.3ml, the liquid discharging range of the third quantitative pump 3 is 5.4ml, the first quantitative pump 1 and the second quantitative pump 2 are respectively used for outputting the first dye solution and the second dye solution, the third quantitative pump 3 is used for outputting a buffer solution, and under the condition that the total volume of the target dye mixed solution is ensured to be greater than 5ml, the minimum third target volume, the minimum fourth target volume and the minimum second target volume among a plurality of values according to the mapping relationship are exemplified, and the description is omitted.

When a first dye solution is required to be prepared: a second dye solution: when the ratio of the buffer solution is 1:1:18, the third target frequency of outputting the first dye solution by the first quantitative pump 1 can be determined to be 1 time by the smallest third target volume in the plurality of the possible values, the fourth target frequency of outputting the second dye solution by the second quantitative pump 2 is determined to be 1 time by the smallest fourth target volume in the plurality of the possible values, and the second target frequency of outputting the buffer solution by the third quantitative pump 3 is determined to be 1 time by the smallest second target volume in the plurality of the possible values.

When a first dye solution is required to be prepared: a second dye solution: when the ratio of the buffer solution is 1:0:18, the third target frequency of outputting the first dye solution by the first quantitative pump 1 can be determined to be 1 time by the smallest third target volume in the plurality of the possible values, the fourth target frequency of outputting the second dye solution by the second quantitative pump 2 is determined to be 0 time by the smallest fourth target volume in the plurality of the possible values, and the second target frequency of outputting the buffer solution by the third quantitative pump 3 is determined to be 1 time by the smallest second target volume in the plurality of the possible values.

When a first dye solution is required to be prepared: a second dye solution: when the ratio of the buffer solution is 0:1:18, the third target frequency of outputting the first dye solution by the first quantitative pump 1 can be determined to be 0 times by the smallest third target volume in the plurality of the possible values, the fourth target frequency of outputting the second dye solution by the second quantitative pump 2 is determined to be 1 time by the smallest fourth target volume in the plurality of the possible values, and the second target frequency of outputting the buffer solution by the third quantitative pump 3 is determined to be 1 time by the smallest second target volume in the plurality of the possible values.

When a first dye solution is required to be prepared: a second dye solution: when the ratio of the buffer solution is 0:2:18, it can be determined that the third target number of times that the first quantitative pump 1 outputs the first dye solution is 0, the fourth target number of times that the second quantitative pump 2 outputs the second dye solution is 2, and the second target number of times that the third quantitative pump 3 outputs the buffer solution is 1.

When a first dye solution is required to be prepared: a second dye solution: when the ratio of the buffer solution is 1:2:18, the third target frequency of outputting the first dye solution by the first quantitative pump 1 can be determined to be 1 time by the smallest third target volume in the plurality of the possible values, the fourth target frequency of outputting the second dye solution by the second quantitative pump 2 is determined to be 2 times by the smallest fourth target volume in the plurality of the possible values, and the second target frequency of outputting the buffer solution by the third quantitative pump 3 is determined to be 1 time by the smallest second target volume in the plurality of the possible values.

Other objectives than, for example, the first dye liquor: a second dye solution: the volume ratio of the buffers is 0:0:18, 2:1:18 or 2:2: 18.

340: and controlling the quantitative pump assembly 10 to stop discharging the liquor after the times of outputting the first dye liquor, the second dye liquor and the buffer liquor are respectively the corresponding target times.

When a first dye solution is required to be prepared: a second dye solution: when the ratio of the buffer solution is 1:1:18, controlling the third quantitative pump 3 to output the buffer solution into the container 20 filled with the cell sample, when the output amount of the buffer solution is about 1ml, simultaneously outputting 0.3ml of first dye solution and 0.3ml of second dye solution into the container 20 filled with the cell sample by the first quantitative pump 1 and the second quantitative pump 2, and finishing the output of the buffer solution after the output of the first dye solution and the second dye solution is finished.

When a first dye solution is required to be prepared: a second dye solution: when the ratio of the buffer solution is 1:0:18, the third quantitative pump 3 is controlled to output the buffer solution to the container 20 containing the cell sample, when the output liquid amount of the buffer solution is about 1ml, the first quantitative pump 1 outputs 0.3ml of first dye solution to the container 20 containing the cell sample, and after the output of the first dye solution is finished, the output of the buffer solution is also finished.

When a first dye solution is required to be prepared: a second dye solution: when the ratio of the buffer solution is 0:1:18, the third quantitative pump 3 is controlled to output the buffer solution to the container 20 containing the cell sample, when the output liquid amount of the buffer solution is about 1ml, the second quantitative pump 2 outputs 0.3ml of second dye solution to the container 20 containing the cell sample, and after the output of the second dye solution is finished, the output of the buffer solution is also finished.

When a first dye solution is required to be prepared: a second dye solution: when the ratio of the buffer solution is 0:2:18, the third quantitative pump 3 is controlled to output the buffer solution for the first time, the amount of the buffer solution to the container 20 containing the cell sample for the first time is about 2.7ml by controlling the solution output time of the third quantitative pump 3, and the second quantitative pump 2 outputs 0.3ml of the second dye solution to the container 20 containing the cell sample in the first solution output process of the buffer solution. After the first time of the second dye solution and the buffer solution are both completely transfused, the buffer solution is output for the second time, 2.7ml of the remaining buffer solution in the third quantitative pump 3 is completely transfused, and 0.3ml of the second dye solution is output to the container 20 filled with the cell sample by the second quantitative pump 2 in the process of outputting the buffer solution for the second time.

When a first dye solution is required to be prepared: a second dye solution: when the ratio of the buffer solution is 1:2:18, the third quantitative pump 3 is controlled to output the buffer solution for the first time, the amount of the buffer solution to the container 20 containing the cell sample for the first time is about 2.7ml by controlling the liquid discharging time of the third quantitative pump 3, and the second quantitative pump 2 outputs 0.3ml of the second dye solution to the container 20 containing the cell sample in the first liquid discharging process of the buffer solution. After the first second dye solution and the buffer solution are completely delivered, the buffer solution is delivered for the second time, 2.7ml of the buffer solution left in the third quantitative pump 3 is completely delivered, and the first quantitative pump 1 and the second quantitative pump 2 simultaneously deliver 0.3ml of the first dye solution and the second dye solution to the container 20 filled with the cell sample in the process of delivering the buffer solution for the second time.

Other target proportions can be achieved by draining in a similar way, such as first dye liquor: a second dye solution: the volume ratio of the buffers is 0:0:18, 2:1:18 or 2:2: 18.

350: and mixing the first dye solution, the second dye solution and the buffer solution to form a target dye mixed solution.

The content of step 250 can be referred to in this step, and is not described herein again.

In the embodiment of the invention, the target volume proportion of a first dye solution, a second dye solution and a buffer solution is determined, a first fixed displacement pump 1 is controlled to suck the first dye solution, a second fixed displacement pump 2 is controlled to suck the second dye solution, a third fixed displacement pump 3 is controlled to suck the buffer solution, a third target frequency of outputting the first dye solution, a fourth target frequency of outputting the second dye solution and a second target frequency of outputting the buffer solution by a fixed displacement pump assembly 10 are determined according to the target volume proportion, the volume of a liquid chamber of the first fixed displacement pump 1, the volume of a liquid chamber of the second fixed displacement pump 2 and the volume of a liquid chamber of the third fixed displacement pump 3, the fixed displacement pump assembly 10 is controlled to stop discharging the dye solution after the first dye solution, the second dye solution and the buffer solution are respectively output for the corresponding target frequencies, the first dye solution, the second dye solution and the buffer solution are mixed to form a target dye mixed solution, and thus the third target frequency of outputting the first dye solution by the first fixed displacement pump 1 is controlled, The fourth target times of the second dye solution output by the second quantitative pump 2 and the second target times of the buffer solution output by the third quantitative pump 3 are mixed to form dye mixed liquor with different concentrations, the concentration types of the dye mixed liquor configured by the push piece dyeing machine 100 are increased, so that the dye mixed liquor with various concentrations is configured, the difference of dyeing depth of the cell sample is realized, and different clinical requirements are met; meanwhile, the time coincidence of the output dye liquor and the output buffer liquor is controlled to ensure that the buffer liquor and the dye liquor can be uniformly mixed when flowing out of the dyeing box.

In addition, the configuration methods provided in the second and third embodiments are applied to the same fixed displacement pump assembly 10, so that the push piece dyeing machine 100 having the fixed displacement pump assembly 10 can realize the configuration of the dye mixed liquor for single dyeing in the second embodiment, and can also realize the configuration of the dye mixed liquor for double dyeing in the third embodiment, that is, the same equipment can realize the dye mixed liquor for single dyeing with multiple concentrations and the dye mixed liquor for double dyeing with multiple concentrations.

As shown in fig. 7, a fourth embodiment of the present invention provides a method for preparing a dye mixture, which is applied to a push-piece dyeing machine 100, a cell dyeing machine, and the like shown in fig. 3 and 4. The present invention will be described by taking an example in which the method for preparing a dye mixture is applied to a slide dyeing machine 100. The push-piece dying machine 100 includes a dosing pump assembly 10. The method for preparing the dye mixed liquid provided by the embodiment increases the concentration types of the dye mixed liquid prepared by the push piece dyeing machine 100 so as to prepare the dye mixed liquid with various concentrations, thereby realizing the difference of dyeing depth of the cell sample and meeting different clinical requirements.

The method of the present embodiment is similar to that of the first embodiment, and the main difference is that the target times of outputting the dye liquor and the buffer liquor by the quantitative pump assembly 10 are determined according to the target concentration of the target dye mixed liquor.

410: and determining the target concentration of the target dye mixed liquor.

In one embodiment, the push piece dyeing machine may be configured to set a plurality of concentrations of the target dye mixture in advance. The target dye mixed solution may include a dye solution and a buffer solution. Various concentrations include, but are not limited to, 5.26%, 10%, 14.28%, or 18.18%, among others.

The operator selects a target concentration to be configured currently among a plurality of concentrations set in advance. For example, the target concentration to be currently configured is selected to be 10%.

In another embodiment, the operator inputs the target concentration currently to be configured in the push-piece dyeing machine, for example, 10% in the push-piece dyeing machine.

420: and controlling the quantitative pump assembly to respectively suck the dye liquor and the buffer liquor.

This step can be referred to as step 120 and step 220 and will not be described herein again.

430: and determining the target times respectively corresponding to the dye liquor and the buffer liquor output by the quantitative pump component according to the target concentration.

In one embodiment, a target volume ratio of the dye solution to the buffer solution is determined based on the target concentration; and determining a first target number of times of outputting the dye liquor and a second target number of times of outputting the buffer liquor by the quantitative pump assembly 10 according to the target volume proportion, the volume of the liquor chamber for storing the dye liquor and the volume of the liquor chamber for storing the buffer liquor.

Specifically, the target volume ratio of the dye solution and the buffer solution in the target dye mixed solution is determined according to the target volume ratio of the target dye mixed solution. For example, the target volume ratio of the target dye mixture is 5.26%, and the target volume ratio of the dye solution to the buffer solution is 1: 18. The target volume ratio of the target dye mixed liquor is 10%, and the target volume ratio of the dye liquor to the buffer liquor is 1: 9. The target volume ratio of the target dye mixed liquor is 14.28%, and the target volume ratio of the dye liquor to the buffer liquor is 1: 6. The target volume ratio of the target dye mixed liquor is 18.18%, and the target volume ratio of the dye liquor to the buffer liquor is 2: 9.

For the specific steps of determining the first target number of times of outputting the dye solution and the second target number of times of outputting the buffer solution by the quantitative pump assembly 10 according to the target volume ratio, the volume of the solution chamber storing the dye solution and the volume of the solution chamber storing the buffer solution, reference may be made to step 130 and step 230, which are not described herein again.

In another embodiment, the effluent range of the fixed displacement pump for sucking the dye solution and the effluent range of the fixed displacement pump for sucking the buffer solution are determined, a mapping relation between target concentrations of the dye solution and the buffer solution and target times corresponding to the dye solution and the buffer solution output by the fixed displacement pump assembly is established according to the effluent range of the fixed displacement pump for sucking the dye solution and the effluent range of the fixed displacement pump for sucking the buffer solution, and the target times corresponding to the dye solution and the buffer solution output by the fixed displacement pump assembly are controlled according to the mapping relation.

In this embodiment, a quantitative pump with a liquid discharge range of 0.3ml is used to suck the dye solution, and a quantitative pump with a liquid discharge range of 5.4ml is used to suck the buffer solution for illustration, which will not be described again in the following. The mapping relation between the target concentrations of the dye solution and the buffer solution and the target times corresponding to the dye solution and the buffer solution output by the quantitative pump assembly is that the target concentration C of the target dye mixed solution is (0.3 x n)/(0.3 x n +5.4 x m), wherein n is the target times corresponding to the dye solution output by the quantitative pump assembly, and m is the target times corresponding to the buffer solution output by the quantitative pump assembly. And determining a plurality of possible values of n and a plurality of possible values of m according to the mapping relation and the target concentration. In one embodiment, in the case that the total volume (0.3 × n +5.4 × m) of the target dye mixture is equal to or greater than the preset volume, the minimum value is taken from the plurality of possible values of n and the minimum value is taken from the plurality of possible values of m, so as to save the usage amount of the dye solution and the buffer solution on the basis of ensuring the dyeing effect on the cell sample. In this embodiment, a minimum value is taken from a plurality of available values of n and a minimum value is taken from a plurality of available values of m for example when the total volume of the target dye mixed solution is greater than or equal to 5ml, and details are not repeated in the following. Of course, the preset volume is not limited in particular, and in other embodiments, the preset volume may be 6ml, 7ml, or 10ml, etc.

When the target concentration of the target dye mixture is 10%, the target times corresponding to the first dye solution and the first buffer solution can be determined to be 2 times and 1 time, respectively. When the target concentration of the target dye mixture is 5.26%, the target times corresponding to the first dye solution and the first buffer solution can be determined to be 1 time and 1 time, respectively. When the target concentration of the target dye mixture is 14.28%, the target times corresponding to the first dye solution and the first buffer solution can be determined to be 3 times and 1 time, respectively. When the target concentration of the target dye mixture is 18.18%, the target times corresponding to the first dye solution and the first buffer solution can be determined to be 4 times and 1 time, respectively. Of course, in other embodiments, n may not take the minimum value among the plurality of possible values and m may not take the minimum value among the plurality of possible values. For example, when the target concentration of the target dye mixture is 10%, the target times corresponding to the first dye solution and the first buffer solution are 4 times and 2 times, respectively, or the target times corresponding to the first dye solution and the first buffer solution are 8 times and 4 times, respectively, and so on. The same is true for other concentrations, which are not further enumerated herein.

440: and controlling the quantitative pump assembly to stop discharging the liquor after the times of outputting the dye liquor and the buffer liquor are respectively the corresponding target times.

This step can refer to step 140 and step 240, and will not be described herein.

450: and mixing the dye solution and the buffer solution to form a target dye mixed solution.

This step can refer to step 150 and step 250, which are not described herein.

The embodiment provided by the invention has the advantages that the target concentration of the target dye mixed liquid is determined, the quantitative pump assembly is controlled to respectively suck the dye liquid and the buffer liquid according to the target dye mixed liquid, the target times corresponding to the dye liquid and the buffer liquid output by the quantitative pump assembly are determined according to the target concentration, the quantitative pump assembly is controlled to stop discharging the liquid after the times of outputting the dye liquid and the buffer liquid are respectively the corresponding target times, and the dye liquid and the buffer liquid are mixed to form the target dye mixed liquid.

Further, here, it is to be noted that: an embodiment of the present invention further provides a computer-readable storage medium, where a computer program executed by the aforementioned data processing apparatus is stored in the computer-readable storage medium, and the computer program includes program instructions, and when the processor executes the program instructions, the method described in any one of the first to fourth embodiments can be executed, so that details are not repeated here. In addition, the beneficial effects of the same method are not described in detail. For technical details not disclosed in the embodiments of the computer-readable storage medium according to the present invention, reference is made to the description of the method embodiments of the present invention.

The features mentioned above in the description, the claims and the drawings can be combined with one another in any desired manner, insofar as they are within the scope of the invention. While the foregoing is directed to embodiments of the present invention, it will be appreciated by those skilled in the art that various changes and modifications may be made without departing from the principles of the invention, and it is intended that all such changes and modifications be considered as within the scope of the invention.

Claims

1. A preparation method of dye mixed liquid is applied to a push piece dyeing machine, and is characterized in that the push piece dyeing machine comprises a quantitative pump assembly, and the method comprises the following steps:

controlling the quantitative pump assembly to respectively suck the dye liquor and the buffer solution;

2. The method of claim 1, wherein determining the target number of times that the quantitative pump assembly outputs the dye solution and the buffer solution respectively according to the target volume ratio comprises:

and determining a first target number of times that the quantitative pump assembly outputs the dye solution and a second target number of times that the quantitative pump assembly outputs the buffer solution according to the target volume proportion, the volume of the liquid chamber for storing the dye solution and the volume of the liquid chamber for storing the buffer solution.

3. The method of claim 2, wherein said determining a first target number of times that the fixed displacement pump assembly outputs the dye solution and a second target number of times that the fixed displacement pump assembly outputs the buffer solution based on the target volume ratio, the volume of the solution chamber storing the dye solution, and the volume of the solution chamber storing the buffer solution comprises:

determining a first target volume of the dye solution and a second target volume of the buffer solution output by the quantitative pump assembly according to the target volume proportion, the volume of the solution chamber for storing the dye solution and the volume of the solution chamber for storing the buffer solution; wherein the first target volume is a first multiple of a volume of a liquor chamber storing the liquor, the first multiple being an integer greater than or equal to 0; the second target volume is a second multiplier of the volume of the liquid chamber storing the buffer, the second multiplier is an integer greater than or equal to 0, and the sum of the first multiplier and the second multiplier is an integer greater than or equal to 1;

determining the first time value as a first target number of times that the fixed displacement pump assembly outputs the dye liquor;

determining the second multiplier value as a second target number of times the dosing pump assembly outputs the buffer.

4. The method according to any one of claims 1 to 3, wherein the quantitative pump assembly comprises a first quantitative pump and a second quantitative pump, and the controlling the quantitative pump assembly to respectively suck the dye solution and the buffer solution according to the target dye mixture comprises:

and controlling the first quantitative pump to pump the dye solution and controlling the second quantitative pump to pump the buffer solution according to the target dye mixed solution.

5. The method according to any one of claims 1 to 3, wherein the quantitative pump assembly comprises a first quantitative pump, a second quantitative pump and a third quantitative pump, and the controlling of the quantitative pump assembly to respectively suck the dye solution and the buffer solution according to the target dye mixed solution comprises:

and controlling the first quantitative pump and the second quantitative pump to suck the dye solution and controlling the third quantitative pump to suck the buffer solution according to the target dye mixed solution.

6. The method of any one of claims 1-3, wherein the fixed displacement pump assembly comprises a first fixed displacement pump, a second fixed displacement pump, and a third fixed displacement pump; the target dye mixed solution comprises a first dye solution, a second dye solution and a buffer solution; the control according to the mixed liquid of target dyestuff the ration pump package spare absorbs respectively the dye liquor with the buffer solution, include:

and controlling the first quantitative pump to suck the first dye solution, controlling the second quantitative pump to suck the second dye solution, and controlling the third quantitative pump to suck the buffer solution.

7. The method as claimed in claim 4, wherein said controlling said dosing pump assembly to stop draining after the number of times of outputting said dye liquor and said buffer liquor is respectively the corresponding target number of times comprises:

when the second quantitative pump adds a first volume of the buffer solution into a container, controlling the first quantitative pump to stop discharging the buffer solution after the dye solution is added into the container for the corresponding target times, and controlling the second quantitative pump to stop discharging the buffer solution after a second volume of the buffer solution is added into the container, wherein the sum of the first volume and the second volume is the sum of the volumes of the buffer solution output by the second quantitative pump for the corresponding target times.

8. The method as claimed in claim 4, wherein said controlling said dosing pump assembly to stop draining after the number of times of outputting said dye liquor and said buffer liquor is respectively the corresponding target number of times comprises:

in the process of controlling the second quantitative pump to add the first volume of the buffer solution into the container, controlling the first quantitative pump to stop discharging the solution after the first number of times of adding the dye solution into the container;

and in the process of controlling the second quantitative pump to add a second volume of the buffer solution into the container, controlling the first quantitative pump to stop discharging the buffer solution after the dye solution is added into the container for the second time, wherein the sum of the first time and the second time is the target time corresponding to the dye solution output by the first quantitative pump, and the sum of the first volume and the second volume is the sum of the volumes of the buffer solution output by the second quantitative pump through the corresponding target time.

9. The method as claimed in claim 4, wherein said controlling said dosing pump assembly to stop draining after the number of times of outputting said dye liquor and said buffer liquor is respectively the corresponding target number of times comprises:

when the first quantitative pump adds a first volume of the dye solution into a container, controlling the second quantitative pump to stop discharging the dye solution after the buffer solution with the second target number of times is added into the container, and controlling the first quantitative pump to stop discharging the dye solution after a second volume of the dye solution is added into the container, wherein the sum of the first volume and the second volume is the sum of the volumes of the dye solution output by the first quantitative pump for the corresponding target number of times.

10. The method of claim 1, wherein the quantitative pump assembly comprises at least one quantitative pump, and the controlling of the quantitative pump assembly stops the effluent after the number of times of outputting the dye solution and the buffer solution is respectively the corresponding target number of times, further comprises:

and applying pressure to the liquid chamber of the quantitative pump to output the reagent liquid in the liquid chamber.

11. The method of claim 10, wherein the fixed displacement pump comprises a gas chamber, a liquid chamber, and a flexible diaphragm separating the gas chamber from the liquid chamber;

the applying pressure to the liquid chamber of the fixed displacement pump includes:

inflating the gas chamber such that gas within the gas chamber exerts pressure on the liquid chamber of the dosing pump;

or opening an electromagnetic valve so that the electromagnetic valve pushes the elastic membrane to apply pressure to the liquid chamber of the fixed displacement pump.

12. A preparation method of dye mixed liquid is applied to a push piece dyeing machine, and is characterized in that the push piece dyeing machine comprises a quantitative pump assembly, and the method comprises the following steps:

determining the target concentration of the target dye mixed liquor;

13. A computer storage medium, characterized in that the computer storage medium stores a computer program comprising program instructions which, when executed by a processor, perform the method of any one of claims 1 to 12.