CN112816279B - Sample extraction and separation device for corn flour biotoxin detection - Google Patents
Sample extraction and separation device for corn flour biotoxin detection Download PDFInfo
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- CN112816279B CN112816279B CN202011518952.4A CN202011518952A CN112816279B CN 112816279 B CN112816279 B CN 112816279B CN 202011518952 A CN202011518952 A CN 202011518952A CN 112816279 B CN112816279 B CN 112816279B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B11/00—Feeding, charging, or discharging bowls
- B04B11/04—Periodical feeding or discharging; Control arrangements therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
Abstract
The invention discloses a sample extraction and separation device for corn meal biotoxin detection, which comprises a separation box and a box cover positioned on one side of the top of the separation box. This a sample extraction separator for maize flour biotoxin detects, through when reinforced maize flour, carry out the abrasive material to maize flour in advance and handle, guarantee to get into the inside maize flour thickness of centrifuging tube unanimous, improve the extraction separation effect of sample, in addition through the mechanism that draws that sets up, use centrifugal force to carry out centrifugal treatment to every centrifuging tube inside mixture, until the inside upper and lower layering of centrifuging tube, close driving motor, the driving motor output shaft drives the centrifuge shelf and rotates under a plurality of centrifuging tube is located a plurality of catheter, extract through the inside supernatant of liquid suction pipe with a plurality of centrifuging tube at last, obtain virus detection sample, the separation efficiency of drawing to the sample has effectively been improved, and the phenomenon of sample emulsification has been avoided appearing.
Description
Technical Field
The invention relates to the technical field of sample detection, in particular to a sample extraction and separation device for corn meal biotoxin detection.
Background
The corn flour has different thicknesses, contains rich nutrients, is divided into yellow corn flour and white corn flour according to colors, is also divided into waxy corn flour according to types, has a plurality of corn flour foods, contains a large amount of lecithin, linoleic acid, grain alcohol, vitamin E, cellulose and the like, has various health-care effects of reducing blood pressure, reducing blood fat, resisting arteriosclerosis, preventing intestinal cancer, maintaining beauty and keeping young, delaying senility and the like, is also a suitable good product for diabetics, completely retains the nutritional ingredients and conditioning functions of the corn, and overcomes the defects of poor mouthfeel and difficult digestion of coarse grain flour foods.
In traditional testing process, biotoxin among the maize flour sample detects extraction efficiency and is lower, because maize flour is grain cereal, maize flour's water content is lower, smash after easily form the turbid liquid with the extract, the extract that finally obtains is the solid-liquid suspending agent, difficult filtration through filter paper, sample emulsification phenomenon often takes place, the layering that stews needs to consume a large amount of time, and then influence subsequent filtration and solid-phase extraction step, for solving above problem, the technical personnel in the field have provided a sample extraction separator for maize flour biotoxin detects.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a sample extraction and separation device for corn meal biotoxin detection, wherein an abrasive material component is arranged in the sample extraction and separation device, the abrasive material component comprises an abrasive material motor, an abrasive material plate, a fixing frame, a filter screen, a scraping rod and a scraping plate, the abrasive material motor is fixedly connected above the interior of the feeding frame, one end of an output shaft of the abrasive material motor penetrates through the feeding frame and extends into the feeding frame, the fixing frame is fixedly connected inside the feeding frame, the filter screen is fixedly connected inside the fixing frame, the abrasive material plate is fixedly connected at one end of the output shaft of the abrasive material motor, which extends into the interior of the feeding frame, a blanking hole is formed in the interior of the abrasive material plate, an abrasive material bulge is formed in the bottom surface of the abrasive material plate, the bottom surface of the abrasive material plate is in contact with the upper surface of the filter screen, when corn meal is fed, the method comprises the steps of carrying out abrasive treatment on corn flour in advance, ensuring that the corn flour entering the centrifuge tubes are consistent in thickness, improving the extraction and separation effects of samples, carrying out centrifugal treatment on a mixture in each centrifuge tube by using a centrifugal force through an arranged extraction mechanism until the mixture in each centrifuge tube is layered up and down, turning off a driving motor, driving a centrifugal frame to rotate to a position under a plurality of centrifuge tubes by an output shaft of the driving motor, and finally extracting supernate in the centrifuge tubes through an extraction tube to obtain virus detection samples, effectively improving the extraction and separation efficiency of the samples, avoiding the phenomenon of sample emulsification, solving the problems that in the traditional detection process, the biotoxin detection and extraction efficiency in the corn flour samples is low, the corn flour is grain grains and has low water content, and the corn flour is easy to form a suspension with an extracting solution after being crushed, the finally obtained extracting solution is a solid-liquid suspending agent, is difficult to filter through filter paper, and often generates a sample emulsification phenomenon, and a large amount of time is consumed for standing and layering, so that the problems of subsequent filtering and solid-phase extraction steps are influenced.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: a sample extraction and separation device for corn flour biotoxin detection comprises a separation box and a box cover located on one side of the top of the separation box, wherein one side of the top of the separation box is hinged with one side of the box cover through a hinge, a fixed cylinder is fixedly connected inside the separation box, a feeding mechanism and an extraction mechanism are respectively arranged inside the fixed cylinder, the feeding mechanism is located above the inside of the fixed cylinder, the extraction mechanism is located below the inside of the fixed cylinder, and a driving assembly is arranged below the fixed cylinder;
the feeding mechanism comprises a feeding frame, a feeding pipe, an abrasive component, discharging pipes, a liquid pumping pipe and a liquid guide pipe, the surface of the feeding frame is movably connected with the inner wall of the fixed cylinder, the left side of the top of the feeding frame is communicated with the feeding pipe, one end of the feeding pipe penetrates through the feeding frame and extends into the feeding frame, the abrasive component is arranged in the feeding frame, the bottom of the feeding frame is communicated with the plurality of discharging pipes which are distributed in an annular and equidistant mode, and electromagnetic valves are arranged in the plurality of discharging pipes;
draw mechanism including centrifugal frame, centrifuging tube, dwang and spacing hole, the dwang is located the bottom rotation of feeding frame, and the fixed surface of dwang is connected with the centrifugal frame, the spacing hole of a plurality of has been seted up to the inside equidistance of centrifugal frame, and the inside in the spacing hole of a plurality of all is provided with the centrifuging tube.
Preferably, the bottom of the feeding frame is provided with a plurality of liquid guide pipes in an annular and equidistant mode, the right side of the top of the feeding frame is provided with a liquid suction pipe, and one ends of the liquid guide pipes are communicated with the bottom end of the liquid suction pipe.
Preferably, the abrasive material subassembly includes abrasive material motor, abrasive material board, mount, filter screen, scrapes the material pole and scrapes the flitch, the inside top fixedly connected with abrasive material motor of feeding frame, and the one end of abrasive material motor output shaft runs through the feeding frame and extend to the inside of feeding frame, the inside fixedly connected with mount of feeding frame, and the inside fixedly connected with filter screen of mount, abrasive material motor output shaft extends to the inside one end fixedly connected with abrasive material board of feeding frame, and the blanking hole has been seted up to the inside of abrasive material board, the abrasive material arch has been seted up to the bottom surface of abrasive material board, and the upper surface contact of the bottom surface of abrasive material board and filter screen.
Preferably, the middle part fixedly connected with scraper bar of abrasive material board bottom, and the bottom of scraper bar runs through the filter screen and extends to the below of filter screen, the below fixedly connected with scraper plate on scraper bar surface, and the bottom surface of scraper plate and the bottom contact of feeding frame inner wall.
Preferably, drive assembly includes fixing base, link and driving motor, the bottom fixedly connected with link of solid fixed cylinder, and the bottom fixedly connected with fixing base of link, the middle part fixedly connected with driving motor of link bottom, and the one end through connection frame of driving motor output shaft and solid fixed cylinder and extend to the inside of solid fixed cylinder, the bottom fixed connection of shaft coupling and dwang is passed through to the inside one end of driving motor output shaft.
Preferably, two servo electric cylinders are symmetrically arranged on two sides inside the fixed cylinder, and one ends of driving shafts of the two servo electric cylinders are fixedly connected with two sides of the bottom of the feeding frame respectively.
Preferably, the working method of the sample extraction and separation device for corn meal biotoxin detection is as follows:
step one, opening a box cover and simultaneously opening two servo electric cylinder switches, wherein two servo electric cylinder driving shafts push a feeding frame to move upwards in a fixed cylinder, adding equal amount of extracting solution into a plurality of centrifuge tubes, controlling the two servo electric cylinder driving shafts to reset, and moving the feeding frame to the upper part of a centrifuge frame;
step two, corn flour is added into the feeding frame through the feeding pipe, the corn flour falls above the grinding plate, the corn flour falls above the filter screen through a blanking hole in the grinding plate, the grinding motor is started, an output shaft of the grinding motor drives the grinding plate to rotate above the filter screen, grinding material protrusions on the bottom surface of the grinding plate further crush the corn flour on the filter screen, the corn flour penetrates through the filter screen and falls inside the feeding frame, meanwhile, the scraping plate is driven by the scraping rod to rotate, the corn flour in the feeding frame is scraped into the discharging pipes through the scraping plate until the same amount of corn flour is filled into each discharging pipe, and the rest corn flour is scraped to one side to be placed;
step three, starting a driving assembly, driving an output shaft of a driving motor to drive the whole centrifugal frame to rotate, driving a centrifugal tube to move to the position under each discharging tube through the centrifugal frame, synchronously opening electromagnetic valves inside the discharging tubes, enabling corn flour inside the discharging tubes to fall into the centrifugal tubes under the action of gravity, closing the electromagnetic valves, completing charging of the centrifugal tubes, starting two servo electric cylinder switches, continuously pushing the feeding frame to lift upwards through two servo electric cylinder driving shafts, and blocking a tube opening of each centrifugal tube;
fourthly, the output shaft of the driving motor drives the centrifugal frames to rotate at the rotating speed of 12000-16000r/min, the mixture in each centrifugal tube is subjected to centrifugal treatment until the upper part and the lower part of the centrifugal tube are layered, the driving motor is turned off, and the output shaft of the driving motor drives the centrifugal frames to rotate until a plurality of centrifugal tubes are positioned under a plurality of liquid guide tubes;
step five, open two servo electric jar switches, let the feeding frame rebound at first, open the mouth of pipe of every centrifuging tube, take the feeding frame rebound through two servo electric jars after that, until the inside of every catheter bottom entering centrifuging tube of feeding frame bottom, extract the inside supernatant of a plurality of centrifuging tube through the liquid suction pipe, obtain virus detection sample, two servo electric jar drive shafts stretch out, take out every centrifuging tube at last, collect the processing to the inside material of centrifuging tube.
(III) advantageous effects
The invention provides a sample extraction and separation device for corn meal biotoxin detection. Compared with the prior art, the method has the following beneficial effects:
this a sample extraction separator for maize meal biotoxin detects, through set up the abrasive material subassembly in sample extraction separator's inside, the abrasive material subassembly includes abrasive material motor, abrasive material board, mount, filter screen, scrapes the material pole and scrapes the flitch, the inside top fixedly connected with abrasive material motor of feeding frame, and the inside that the one end of abrasive material motor output shaft runs through the feeding frame and extends to the feeding frame, the inside fixedly connected with mount of feeding frame, and the inside fixedly connected with filter screen of mount, the one end fixedly connected with abrasive material board that the abrasive material motor output shaft extends to the feeding frame inside, and the inside blanking hole of having seted up of abrasive material board, the abrasive material arch has been seted up to the bottom surface of abrasive material board, and the upper surface contact of bottom surface and filter screen of abrasive material board, when feeding in advance maize meal, carries out the abrasive material processing to maize in advance, guarantees that the maize meal that gets into the inside of centrifuging tube is thick and thin unanimous, improve the extraction separation effect of sample, in addition through the mechanism that draws that sets up, use centrifugal force to carry out centrifugal treatment to every inside mixture of centrifuge tube, until the inside upper and lower layering of centrifuge tube, close driving motor, the driving motor output shaft drives the centrifugation frame and rotates under a plurality of centrifuging tube is located a plurality of catheter, extract the inside supernatant of a plurality of centrifuge tube through the liquid suction pipe at last, obtain virus detection sample, effectively improved the extraction separation efficiency to the sample, and avoided the phenomenon of sample emulsification to appear.
Drawings
FIG. 1 is a schematic diagram of the structure of a sample extraction and separation device for corn meal biotoxin detection according to the present invention;
FIG. 2 is a cross-sectional view of a stationary barrel construction of the present invention;
FIG. 3 is a cross-sectional view of the feed carriage structure of the present invention;
FIG. 4 is a bottom view of the feeder frame structure of the present invention;
FIG. 5 is a schematic view of the driving assembly structure of the present invention.
In the figure, 1, a feeding mechanism; 11. a feeding frame; 12. a feed pipe; 13. an abrasive component; 131. an abrasive motor; 132. a sheet of abrasive material; 133. a fixed mount; 134. filtering with a screen; 135. a scraping rod; 136. a scraping plate; 14. a discharge pipe; 15. a liquid pumping pipe; 16. a catheter; 2. an extraction mechanism; 21. a centrifugal rack; 22. centrifuging the tube; 23. rotating the rod; 24. a limiting hole; 3. a drive assembly; 31. a fixed seat; 32. a connecting frame; 33. a drive motor; 4. a separation box; 5. a box cover; 6. a fixed cylinder; 7. and a servo electric cylinder.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Referring to fig. 1-5, a sample extraction and separation device for corn meal biotoxin detection comprises a separation box 4 and a box cover 5 located on one side of the top of the separation box 4, wherein one side of the top of the separation box 4 is hinged to one side of the box cover 5 through a hinge, a fixed cylinder 6 is fixedly connected inside the separation box 4, a feeding mechanism 1 and an extraction mechanism 2 are respectively arranged inside the fixed cylinder 6, the feeding mechanism 1 is located above the inside of the fixed cylinder 6, the extraction mechanism 2 is located below the inside of the fixed cylinder 6, and a driving assembly 3 is arranged below the fixed cylinder 6;
the feeding mechanism 1 comprises a feeding frame 11, a feeding pipe 12, an abrasive component 13, a discharging pipe 14, a liquid pumping pipe 15 and a liquid guide pipe 16, the surface of the feeding frame 11 is movably connected with the inner wall of the fixed cylinder 6, the left side of the top of the feeding frame 11 is communicated with the feeding pipe 12, one end of the feeding pipe 12 penetrates through the feeding frame 11 and extends into the feeding frame 11, the abrasive component 13 is arranged in the feeding frame 11, the bottom of the feeding frame 11 is communicated with a plurality of discharging pipes 14 which are annularly and equidistantly distributed, and electromagnetic valves are arranged in the discharging pipes 14;
The bottom of the feeding frame 11 is annularly and equidistantly provided with a plurality of liquid guide pipes 16, the right side of the top of the feeding frame 11 is provided with a liquid extracting pipe 15, and one end of each of the plurality of liquid guide pipes 16 is communicated with the bottom end of the liquid extracting pipe 15.
The middle part of the bottom of the abrasive plate 132 is fixedly connected with a scraping rod 135, the bottom end of the scraping rod 135 penetrates through the filter screen 134 and extends to the lower part of the filter screen 134, the lower part of the surface of the scraping rod 135 is fixedly connected with a scraping plate 136, and the bottom surface of the scraping plate 136 is in contact with the bottom of the inner wall of the feeding frame 11.
Drive assembly 3 includes fixing base 31, link 32 and driving motor 33, the bottom fixedly connected with link 32 of solid fixed cylinder 6, and the bottom fixedly connected with fixing base 31 of link 32, the middle part fixedly connected with driving motor 33 of link 32 bottom, and the one end through connection frame 32 of driving motor 33 output shaft and solid fixed cylinder 6 and extend to the inside of solid fixed cylinder 6, the bottom fixed connection of shaft coupling and dwang 23 is passed through to the inside one end of driving motor 33 output shaft, the inside bilateral symmetry of solid fixed cylinder 6 is provided with two servo electric jar 7, and the one end of two servo electric jar 7 drive shafts respectively with the both sides fixed connection of feeding frame 11 bottoms.
The working method of the sample extraction and separation device for corn meal biotoxin detection is as follows:
step one, opening a box cover 5 and opening two servo electric cylinders 7, driving shafts of the two servo electric cylinders 7 push a feeding frame 11 to move upwards in a fixed cylinder 6, adding equal amount of extracting solution into a plurality of centrifugal tubes 22, controlling the driving shafts of the two servo electric cylinders 7 to reset, and moving the feeding frame 11 to the upper part of a centrifugal frame 21;
step two, corn flour is added into the feeding frame 11 through the feeding pipe 12, the corn flour falls on the grinding material plate 132 and falls on the filter screen 134 through a blanking hole in the grinding material plate 132, the grinding material motor 131 is started, an output shaft of the grinding material motor 131 drives the grinding material plate 132 to rotate above the filter screen 134, grinding protrusions on the bottom surface of the grinding material plate 132 further grind the corn flour on the filter screen 134, the corn flour penetrates through the filter screen 134 and falls in the feeding frame 11, meanwhile, the scraper plate 136 is driven by the scraper rod 135 to rotate, the corn flour in the feeding frame 11 is scraped into the discharging pipes 14 through the scraper plate 136 until the same amount of corn flour is filled into each discharging pipe 14, and the rest corn flour is scraped to one side to be placed;
step three, starting the driving assembly 3, driving the whole centrifugal frame 21 to rotate by an output shaft of the driving motor 33, driving the centrifugal tubes 22 to move to the position under each discharging tube 14 through the centrifugal frame 21, synchronously opening electromagnetic valves inside the discharging tubes 14, enabling corn flour inside the discharging tubes 14 to fall into the centrifugal tubes 22 under the action of gravity, closing the electromagnetic valves, completing charging of the centrifugal tubes 22, starting the switches of the two servo electric cylinders 7, continuously pushing the feeding frame 11 to be lifted upwards by the driving shafts of the two servo electric cylinders 7, and blocking the tube openings of the centrifugal tubes 22;
fourthly, the output shaft of the driving motor 33 drives the centrifugal frames 21 to rotate at the rotating speed of 12000r/min, the mixture in each centrifugal tube 22 is subjected to centrifugal treatment until the centrifugal tubes 22 are layered up and down, the driving motor 33 is turned off, and the output shaft of the driving motor 33 drives the centrifugal frames 21 to rotate until the centrifugal tubes 22 are positioned under the liquid guide tubes 16;
step five, open two servo electric jar 7 switches, at first let feeding frame 11 upwards move, open the mouth of pipe of every centrifuging tube 22, take feeding frame 11 downstream through two servo electric jars 7, until the inside that every catheter 16 bottom of feeding frame 11 bottom got into centrifuging tube 22, extract the inside supernatant of a plurality of centrifuging tube 22 through liquid suction pipe 15, obtain the virus detection sample, two servo electric jar 7 drive shafts stretch out, take out every centrifuging tube 22 at last, collect the processing to the inside material of centrifuging tube 22.
Example 2
The present embodiment is different from embodiment 1 in that:
the working method of the sample extraction and separation device for corn meal biotoxin detection is as follows:
step one, opening a box cover 5 and opening two servo electric cylinders 7, driving shafts of the two servo electric cylinders 7 push a feeding frame 11 to move upwards in a fixed cylinder 6, adding equal amount of extracting solution into a plurality of centrifugal tubes 22, controlling the driving shafts of the two servo electric cylinders 7 to reset, and moving the feeding frame 11 to the upper part of a centrifugal frame 21;
step two, corn flour is added into the feeding frame 11 through the feeding pipe 12, the corn flour falls on the grinding material plate 132 and falls on the filter screen 134 through a blanking hole in the grinding material plate 132, the grinding material motor 131 is started, an output shaft of the grinding material motor 131 drives the grinding material plate 132 to rotate above the filter screen 134, grinding protrusions on the bottom surface of the grinding material plate 132 further grind the corn flour on the filter screen 134, the corn flour penetrates through the filter screen 134 and falls in the feeding frame 11, meanwhile, the scraper plate 136 is driven by the scraper rod 135 to rotate, the corn flour in the feeding frame 11 is scraped into the discharging pipes 14 through the scraper plate 136 until the same amount of corn flour is filled into each discharging pipe 14, and the rest corn flour is scraped to one side to be placed;
step three, starting the driving assembly 3, driving an output shaft of the driving motor 33 to drive the whole centrifugal frame 21 to rotate, driving the centrifugal tubes 22 to move to the position under each discharging tube 14 through the centrifugal frame 21, synchronously opening electromagnetic valves inside the plurality of discharging tubes 14, enabling corn flour inside the plurality of discharging tubes 14 to fall into the plurality of centrifugal tubes 22 under the action of gravity, closing the electromagnetic valves, completing charging of the centrifugal tubes 22, starting switches of the two servo electric cylinders 7, continuously pushing the feeding frame 11 to be lifted upwards through driving shafts of the two servo electric cylinders 7, and blocking the tube openings of the centrifugal tubes 22;
fourthly, the output shaft of the driving motor 33 drives the centrifugal frames 21 to rotate at the rotating speed of 16000r/min, the mixture in each centrifugal tube 22 is subjected to centrifugal processing until the centrifugal tubes 22 are layered up and down, the driving motor 33 is turned off, and the output shaft of the driving motor 33 drives the centrifugal frames 21 to rotate until the centrifugal tubes 22 are positioned under the liquid guide tubes 16;
step five, opening two servo electric jar 7 switches, at first let feed frame 11 move upwards, open the mouth of pipe of every centrifuging tube 22, take feed frame 11 downstream through two servo electric jars 7, until the inside that gets into centrifuging tube 22 of every catheter 16 bottom of feed frame 11 bottom, extract the supernatant of the inside of a plurality of centrifuging tube 22 through liquid suction pipe 15, obtain virus detection sample, two servo electric jar 7 drive shafts stretch out, take out every centrifuging tube 22 at last, collect the material of the inside of centrifuging tube 22 and handle.
And those not described in detail in this specification are well within the skill of the art.
According to the working principle, when the device is used, the box cover 5 is opened, the two servo electric cylinders 7 are opened, the two servo electric cylinders 7 drive shafts push the feeding frame 11 to move upwards in the fixed barrel 6, the same amount of extracting solution is added into the centrifugal tubes 22, the two servo electric cylinders 7 drive shafts are controlled to reset, and the feeding frame 11 is displaced to the upper side of the centrifugal frame 21;
corn flour is added into the feeding frame 11 through the feeding pipe 12, the corn flour falls on the grinding material plate 132 and falls on the filter screen 134 through a blanking hole in the grinding material plate 132, the grinding material motor 131 is started, an output shaft of the grinding material motor 131 drives the grinding material plate 132 to rotate above the filter screen 134, grinding protrusions on the bottom surface of the grinding material plate 132 further grind the corn flour on the filter screen 134, the corn flour penetrates through the filter screen 134 and falls into the feeding frame 11, meanwhile, the scraper plate 136 is driven by the scraper rod 135 to rotate, the corn flour in the feeding frame 11 is scraped into the discharging pipes 14 through the scraper plate 136 until the same amount of corn flour is filled into each discharging pipe 14, and the rest corn flour is scraped to one side for placement;
starting the driving assembly 3, driving an output shaft of the driving motor 33 to drive the whole centrifugal frame 21 to rotate, driving the centrifugal tubes 22 to move to the position under each discharging tube 14 through the centrifugal frame 21, synchronously opening electromagnetic valves inside the discharging tubes 14, enabling corn flour inside the discharging tubes 14 to fall into the centrifugal tubes 22 under the action of gravity, closing the electromagnetic valves, completing charging of the centrifugal tubes 22, starting the switches of the two servo electric cylinders 7, continuously pushing the feeding frame 11 to be lifted upwards through the driving shafts of the two servo electric cylinders 7, and blocking the tube openings of the centrifugal tubes 22;
the output shaft of the driving motor 33 drives the centrifugal frame 21 to rotate at the rotating speed of 12000-16000r/min, the mixture in each centrifugal tube 22 is centrifuged until the centrifugal tubes 22 are layered up and down, the driving motor 33 is turned off, and the output shaft of the driving motor 33 drives the centrifugal frame 21 to rotate until the centrifugal tubes 22 are positioned under the liquid guide tubes 16;
open two servo electric jar 7 switches, at first let feeding frame 11 upward movement, open the mouth of pipe of every centrifuging tube 22, take feeding frame 11 downstream through two servo electric jars 7 after that, until the inside of 16 bottoms entering centrifuging tubes 22 of every catheter of feeding frame 11 bottoms, extract the inside supernatant of a plurality of centrifuging tube 22 through liquid suction pipe 15, obtain virus detection sample, two servo electric jar 7 drive shafts stretch out, take out every centrifuging tube 22 at last, collect the processing to the inside material of centrifuging tube 22.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. The utility model provides a sample extraction separator for maize meal biotoxin detects, includes separator box (4) and case lid (5) that are located separator box (4) top one side, one side at separator box (4) top is articulated through the hinge with one side of case lid (5), and the inside fixedly connected with solid fixed cylinder (6) of separator box (4), the inside of solid fixed cylinder (6) is provided with feed mechanism (1) respectively and draws mechanism (2), its characterized in that: the feeding mechanism (1) is positioned above the inside of the fixed cylinder (6), the extracting mechanism (2) is positioned below the inside of the fixed cylinder (6), and the driving assembly (3) is arranged below the fixed cylinder (6);
the feeding mechanism (1) comprises a feeding frame (11), a feeding pipe (12), an abrasive component (13), a discharging pipe (14), a liquid pumping pipe (15) and a liquid guide pipe (16), the surface of the feeding frame (11) is movably connected with the inner wall of the fixed cylinder (6), the left side of the top of the feeding frame (11) is communicated with the feeding pipe (12), one end of the feeding pipe (12) penetrates through the feeding frame (11) and extends into the feeding frame (11), the abrasive component (13) is arranged inside the feeding frame (11), the bottom of the feeding frame (11) is communicated with a plurality of discharging pipes (14) which are distributed in an annular and equidistant mode, and electromagnetic valves are arranged inside the plurality of discharging pipes (14);
the extraction mechanism (2) comprises a centrifugal frame (21), centrifugal tubes (22), a rotating rod (23) and limiting holes (24), the rotating rod (23) is positioned at the bottom of the feeding frame (11) and rotates, the surface of the rotating rod (23) is fixedly connected with the centrifugal frame (21), a plurality of limiting holes (24) are formed in the centrifugal frame (21) at equal intervals, and the centrifugal tubes (22) are arranged in the limiting holes (24);
the abrasive component (13) comprises an abrasive motor (131), an abrasive plate (132), a fixing frame (133), a filter screen (134), a scraping rod (135) and a scraping plate (136), an abrasive motor (131) is fixedly connected above the interior of the feeding frame (11), one end of an output shaft of the abrasive motor (131) penetrates through the feeding frame (11) and extends into the feeding frame (11), a fixing frame (133) is fixedly connected inside the feeding frame (11), a filter screen (134) is fixedly connected inside the fixed frame (133), one end of the output shaft of the grinding material motor (131) extending into the feeding frame (11) is fixedly connected with a grinding material plate (132), a blanking hole is formed in the grinding plate (132), a grinding bulge is formed in the bottom surface of the grinding plate (132), and the bottom surface of the grinding plate (132) is in contact with the upper surface of the filter screen (134);
the middle part fixedly connected with of abrasive material board (132) bottom scrapes material pole (135), and scrapes the bottom of material pole (135) and run through filter screen (134) and extend to the below of filter screen (134), scrape the below fixedly connected with on material pole (135) surface and scrape flitch (136), and scrape the bottom contact of the bottom surface of flitch (136) and the bottom of feeding frame (11) inner wall.
2. The sample extraction and separation device for corn meal biotoxin detection as claimed in claim 1, wherein the sample extraction and separation device comprises: the bottom of feeding frame (11) annular equidistance is provided with a plurality of catheter (16), the right side at feeding frame (11) top is provided with liquid suction pipe (15), and the one end of a plurality of catheter (16) all communicates with the bottom of liquid suction pipe (15).
3. The sample extraction and separation device for corn meal biotoxin detection as claimed in claim 2, wherein the sample extraction and separation device comprises: drive assembly (3) are including fixing base (31), link (32) and driving motor (33), the bottom fixedly connected with link (32) of solid fixed cylinder (6), and the bottom fixedly connected with fixing base (31) of link (32), the middle part fixedly connected with driving motor (33) of link (32) bottom, and the one end of driving motor (33) output shaft runs through link (32) and solid fixed cylinder (6) and extends to the inside of solid fixed cylinder (6), the bottom fixed connection of shaft coupling and dwang (23) is passed through to the inside one end of driving motor (33) output shaft.
4. The sample extraction and separation device for corn meal biotoxin detection as claimed in claim 3, wherein the sample extraction and separation device comprises: two servo electric cylinders (7) are symmetrically arranged on two sides inside the fixed cylinder (6), and one ends of driving shafts of the two servo electric cylinders (7) are fixedly connected with two sides of the bottom of the feeding frame (11) respectively.
5. The sample extraction and separation device for corn meal biotoxin detection as claimed in claim 4, is characterized in that: the working method of the sample extraction and separation device for corn meal biotoxin detection is as follows:
opening a box cover (5) and opening switches of two servo electric cylinders (7) at the same time, driving shafts of the two servo electric cylinders (7) push a feeding frame (11) to move upwards in a fixed cylinder (6), adding equal amount of extracting solution into a plurality of centrifugal tubes (22), controlling the driving shafts of the two servo electric cylinders (7) to reset, and moving the feeding frame (11) to the position above a centrifugal frame (21);
secondly, corn flour is fed into the feeding frame (11) through a feeding pipe (12), the corn flour falls on the upper part of the grinding material plate (132), the corn flour is further crushed on the filter screen (134) by the abrasive protrusions on the bottom surface of the abrasive plate (132) after passing through the filter screen (134) and falling into the feeding frame (11), meanwhile, the scraping plates (136) are driven by the scraping rods (135) to rotate, corn flour in the feeding frame (11) is scraped into the discharging pipes (14) through the scraping plates (136) until the same amount of corn flour is filled into each discharging pipe (14), and the rest corn flour is scraped to one side for placement;
step three, starting a driving assembly (3), driving an output shaft of a driving motor (33) to drive the whole centrifugal frame (21) to rotate, driving centrifugal tubes (22) to move to positions under each discharging tube (14) through the centrifugal frame (21), synchronously opening electromagnetic valves inside a plurality of discharging tubes (14), enabling corn flour inside the discharging tubes (14) to fall into the centrifugal tubes (22) under the action of gravity, closing the electromagnetic valves, completing charging of the centrifugal tubes (22), starting two servo electric cylinder (7) switches, continuously pushing the feeding frame (11) to be lifted upwards by driving shafts of the two servo electric cylinders (7), and blocking the tube openings of the centrifugal tubes (22);
fourthly, an output shaft of the driving motor (33) drives the centrifugal frames (21) to rotate at the rotating speed of 12000-16000r/min, the mixture in each centrifugal tube (22) is subjected to centrifugal treatment until the mixture in the centrifugal tubes (22) is layered up and down, the driving motor (33) is turned off, and the output shaft of the driving motor (33) drives the centrifugal frames (21) to rotate until the centrifugal tubes (22) are positioned under the liquid guide pipes (16);
step five, open two servo electric jar (7) switches, at first let feed frame (11) upward movement, open the mouth of pipe of every centrifuging tube (22), take feed frame (11) downward movement through two servo electric jar (7) next, until the inside of entering centrifuging tube (22) of every catheter (16) bottom of feed frame (11) bottom, extract the inside supernatant of a plurality of centrifuging tube (22) through liquid suction pipe (15), obtain virus detection sample, two servo electric jar (7) drive shafts stretch out, take out every centrifuging tube (22) at last, collect the processing to the inside material of centrifuging tube (22).
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| US20140273138A1 (en) * | 2013-03-12 | 2014-09-18 | Leon H. Langhauser | Simultaneous Food And Fuel Corn Refining |
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| EP3174970A1 (en) * | 2014-07-30 | 2017-06-07 | F. Hoffmann-La Roche AG | Automated system for processing particles |
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