CN112674097A - Chitosan oligosaccharide and S-abscisic acid compound sterilization regulator and preparation method thereof - Google Patents
Chitosan oligosaccharide and S-abscisic acid compound sterilization regulator and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a compound sterilization regulator of chitosan oligosaccharide and S-abscisic acid and a preparation method thereof, which comprises the following substances, by weight, 20-30 parts of chitosan oligosaccharide, 25-35 parts of S-abscisic acid, 10-30 parts of amino acid stock solution, 10-20 parts of urea, 15-25 parts of glutamic acid, 20-30 parts of auxiliary agent, 50-80 parts of water and 40-60 parts of ethanol solution. The invention has the advantages that the chitosan oligosaccharide is added into the sterilization regulator, has higher solubility, is completely dissolved in water and is easily absorbed and utilized by organisms, so that the absorption and utilization of the sterilization regulator by crops are improved, the S-abscisic acid can start the stress-resistant gene of the plant, induce and activate the stress-resistant immune system in the plant body, improve the resistance of the plant to cold, drought, plant diseases and insect pests and saline alkali, reduce the using amount of chemical pesticides by applying the S-abscisic acid, and improve the quality of agricultural products.
Description
Technical Field
The invention relates to the technical field of sterilization regulators, in particular to a compound sterilization regulator of chitosan oligosaccharide and S-abscisic acid and a preparation method thereof.
Background
In recent years, in order to obtain high-yield crops, vegetables and fruits, pesticides and chemical fertilizers are used in an overproof manner, so that chemical fertilizer and pesticide residues in agricultural products are overproof, soil and water body pollution is caused, and a series of environmental damage and ecological disasters are caused. Seriously threatens human survival and development. In order to solve the problems, a plurality of biological organic fertilizers and pesticides are developed at home and abroad. The organic mixture formed during the dry distillation of plant material has a variety of biological effects. It was used in japan as a feed additive, a soil deodorant and an organic fertilizer starter at the end of the last century. And compared with a systematic research, the influence of the substances on the germination of vegetable seeds and the growth and development of seedlings is researched. The research of China shows that the seedling damping off and other diseases can be effectively prevented. The organic mixture is used for treating seeds, cuttings and root systems of the Holland chrysanthemum and the marigold, and the germination rate of the seeds, the rooting rate of the cuttings and the root system number of seedlings can be improved. It is also used as a starter culture for biofertilizers. However, the use effect varies greatly from place to place, and even the opposite is reported. The reason is that the prior formulations or fertilizers have the following disadvantages: the plant distillate is not produced according to specific processes such as standard temperature, pressure, processing time and the like, the obtained total mixture is obtained, and interaction among some mixtures weakens the effect on plants; the total nutrient content is not strengthened and can not meet the nutritional requirement of plants; the plant nutrient solution cannot be produced according to the classification of raw materials, and different plant raw materials have different components and different effects on plants. Although some products exist at home and abroad and have a long application history, the method is difficult to adapt to the needs of economic development and ecological environment construction in China.
The plant growth regulator is used in agricultural production to regulate the growth process of crop effectively and to reach the aims of raising yield, improving quality, strengthening crop resistance, etc.
Disclosure of Invention
The invention aims to provide a compound sterilization regulator of chitosan oligosaccharide and S-abscisic acid and a preparation method thereof.
The above object of the present invention is achieved by the following technical solutions:
a compound sterilization regulator of chitosan oligosaccharide and S-abscisic acid comprises, by weight, 20-30 parts of chitosan oligosaccharide, 25-35 parts of S-abscisic acid, 10-30 parts of amino acid stock solution, 10-20 parts of urea, 15-25 parts of glutamic acid, 20-30 parts of an auxiliary agent, 50-80 parts of water and 40-60 parts of ethanol solution.
The invention is further configured to: the compound sterilization regulator is one of suspending agent, oil suspending agent, water dispersible granule and wettable powder.
The invention is further configured to: the compound sterilization regulator comprises, by weight, 30 parts of chitosan oligosaccharide, 35 parts of S-abscisic acid, 30 parts of amino acid stock solution, 20 parts of urea, 25 parts of glutamic acid, 30 parts of auxiliary agent, 80 parts of water and 60 parts of ethanol solution.
The invention is further configured to: the compound sterilization regulator comprises, by weight, 20 parts of chitosan oligosaccharide, 25 parts of S-abscisic acid, 10 parts of amino acid stock solution, 10 parts of urea, 15 parts of glutamic acid, 20 parts of auxiliary agent, 50 parts of water and 40 parts of ethanol solution.
The invention is further configured to: the compound sterilization regulator comprises, by weight, 25 parts of chitosan oligosaccharide, 30 parts of S-abscisic acid, 20 parts of amino acid stock solution, 15 parts of urea, 20 parts of glutamic acid, 25 parts of auxiliary agent, 65 parts of water and 50 parts of ethanol solution.
A preparation method of a compound sterilization regulator of chitosan oligosaccharide and S-abscisic acid comprises the following steps:
(1) preparing S-abscisic acid: performing seed culture and fermentation culture on a strain capable of fermenting to produce S-abscisic acid to obtain fermentation liquor containing the S-abscisic acid, performing plate-frame filtration, membrane filtration, acid precipitation and extraction on the fermentation liquor containing the S-abscisic acid to obtain extract liquor, and performing concentration, crystallization, recrystallization and drying on the extract liquor to obtain an S-abscisic acid finished product with the purity of more than 95%;
(2) adding chitosan oligosaccharide, S-abscisic acid, amino acid stock solution, urea, glutamic acid and an auxiliary agent into an ethanol solution for dissolving;
(3) and (3) adding the solution obtained after the dissolution in the step (2) and the S-abscisic acid prepared in the step (1) into water, and then uniformly stirring to obtain the compound sterilization regulator.
In conclusion, the beneficial technical effects of the invention are as follows:
1. the chitosan oligosaccharide is added into the sterilization regulator, has higher solubility, is completely dissolved in water and is easily absorbed and utilized by organisms, so that the absorption and utilization of crops on the sterilization regulator are improved;
s-abscisic acid can start the resistance gene of plant, induce and activate the immune system in plant body, raise the resistance of plant to cold, drought, pest and alkali, reduce the consumption of chemical pesticide and raise the quality of agricultural product.
Detailed Description
The present invention will be described in further detail with reference to examples.
In the case of the example 1, the following examples are given,
the invention discloses a compound sterilization regulator of chitosan oligosaccharide and S-abscisic acid, which comprises, by weight, 30 parts of chitosan oligosaccharide, 35 parts of S-abscisic acid, 30 parts of amino acid stock solution, 20 parts of urea, 25 parts of glutamic acid, 30 parts of auxiliary agent, 80 parts of water and 60 parts of ethanol solution.
The invention is further configured to: the compound sterilization regulator is one of suspending agent, oil suspending agent, water dispersible granule and wettable powder.
A preparation method of a compound sterilization regulator of chitosan oligosaccharide and S-abscisic acid comprises the following steps:
(1) preparing S-abscisic acid: performing seed culture and fermentation culture on a strain capable of fermenting to produce S-abscisic acid to obtain fermentation liquor containing the S-abscisic acid, performing plate-frame filtration, membrane filtration, acid precipitation and extraction on the fermentation liquor containing the S-abscisic acid to obtain extract liquor, and performing concentration, crystallization, recrystallization and drying on the extract liquor to obtain an S-abscisic acid finished product with the purity of more than 95%;
(2) adding chitosan oligosaccharide, S-abscisic acid, amino acid stock solution, urea, glutamic acid and an auxiliary agent into an ethanol solution for dissolving;
(3) and (3) adding the solution obtained after the dissolution in the step (2) and the S-abscisic acid prepared in the step (1) into water, and then uniformly stirring to obtain the compound sterilization regulator.
Example 2
The invention discloses a compound sterilization regulator of chitosan oligosaccharide and S-abscisic acid, which comprises, by weight, 20 parts of chitosan oligosaccharide, 25 parts of S-abscisic acid, 10 parts of amino acid stock solution, 10 parts of urea, 15 parts of glutamic acid, 20 parts of auxiliary agent, 50 parts of water and 40 parts of ethanol solution.
The invention is further configured to: the compound sterilization regulator is one of suspending agent, oil suspending agent, water dispersible granule and wettable powder.
A preparation method of a compound sterilization regulator of chitosan oligosaccharide and S-abscisic acid comprises the following steps:
(1) preparing S-abscisic acid: performing seed culture and fermentation culture on a strain capable of fermenting to produce S-abscisic acid to obtain fermentation liquor containing the S-abscisic acid, performing plate-frame filtration, membrane filtration, acid precipitation and extraction on the fermentation liquor containing the S-abscisic acid to obtain extract liquor, and performing concentration, crystallization, recrystallization and drying on the extract liquor to obtain an S-abscisic acid finished product with the purity of more than 95%;
(2) adding chitosan oligosaccharide, S-abscisic acid, amino acid stock solution, urea, glutamic acid and an auxiliary agent into an ethanol solution for dissolving;
(3) and (3) adding the solution obtained after the dissolution in the step (2) and the S-abscisic acid prepared in the step (1) into water, and then uniformly stirring to obtain the compound sterilization regulator.
Example 3
The invention discloses a compound sterilization regulator of chitosan oligosaccharide and S-abscisic acid, which comprises, by weight, 25 parts of chitosan oligosaccharide, 30 parts of S-abscisic acid, 20 parts of amino acid stock solution, 15 parts of urea, 20 parts of glutamic acid, 25 parts of an auxiliary agent, 65 parts of water and 50 parts of ethanol solution.
The invention is further configured to: the compound sterilization regulator is one of suspending agent, oil suspending agent, water dispersible granule and wettable powder.
A preparation method of a compound sterilization regulator of chitosan oligosaccharide and S-abscisic acid comprises the following steps:
(1) preparing S-abscisic acid: performing seed culture and fermentation culture on a strain capable of fermenting to produce S-abscisic acid to obtain fermentation liquor containing the S-abscisic acid, performing plate-frame filtration, membrane filtration, acid precipitation and extraction on the fermentation liquor containing the S-abscisic acid to obtain extract liquor, and performing concentration, crystallization, recrystallization and drying on the extract liquor to obtain an S-abscisic acid finished product with the purity of more than 95%;
(2) adding chitosan oligosaccharide, S-abscisic acid, amino acid stock solution, urea, glutamic acid and an auxiliary agent into an ethanol solution for dissolving;
(3) and (3) adding the solution obtained after the dissolution in the step (2) and the S-abscisic acid prepared in the step (1) into water, and then uniformly stirring to obtain the compound sterilization regulator.
The chitosan oligosaccharide is added into the sterilization regulator, has higher solubility, is completely dissolved in water and is easily absorbed and utilized by organisms, so that the absorption and utilization of crops on the sterilization regulator are improved; the S-abscisic acid can start the stress-resistant gene of the plant, induce and activate the stress-resistant immune system in the plant body, improve the resistance of the plant to cold, drought, plant diseases and insect pests and salt and alkali, reduce the use amount of chemical pesticides and improve the quality of agricultural products by applying the S-abscisic acid.
The embodiments of the present invention are preferred embodiments of the present invention, and the scope of the present invention is not limited by these embodiments, so: all equivalent changes made according to the structure, shape and principle of the invention are covered by the protection scope of the invention.
Claims (6)
1. A compound sterilization regulator of chitosan oligosaccharide and S-abscisic acid is characterized in that: the chitosan oligosaccharide-beta-cyclodextrin compound fertilizer is composed of, by weight, 20-30 parts of chitosan oligosaccharide, 25-35 parts of S-abscisic acid, 10-30 parts of amino acid stock solution, 10-20 parts of urea, 15-25 parts of glutamic acid, 20-30 parts of an auxiliary agent, 50-80 parts of water and 40-60 parts of ethanol solution.
2. The compound sterilization regulator of chitosan oligosaccharide and S-abscisic acid as claimed in claim 1, which is characterized in that: the compound sterilization regulator is one of suspending agent, oil suspending agent, water dispersible granule and wettable powder.
3. The compound sterilization regulator of chitosan oligosaccharide and S-abscisic acid as claimed in claim 1, which comprises (by weight parts) chitosan oligosaccharide 30, S-abscisic acid 35, amino acid stock solution 30, urea 20, glutamic acid 25, adjuvant 30, water 80, and ethanol solution 60.
4. The compound sterilization regulator of chitosan oligosaccharide and S-abscisic acid as claimed in claim 1, which comprises 20 parts by weight of chitosan oligosaccharide, 25 parts by weight of S-abscisic acid, 10 parts by weight of amino acid stock solution, 10 parts by weight of urea, 15 parts by weight of glutamic acid, 20 parts by weight of auxiliary agent, 50 parts by weight of water and 40 parts by weight of ethanol solution.
5. The compound sterilization regulator of chitosan oligosaccharide and S-abscisic acid as claimed in claim 1, which comprises 25 parts by weight of chitosan oligosaccharide, 30 parts by weight of S-abscisic acid, 20 parts by weight of amino acid stock solution, 15 parts by weight of urea, 20 parts by weight of glutamic acid, 25 parts by weight of auxiliary agent, 65 parts by weight of water and 50 parts by weight of ethanol solution.
6. The preparation method of the compound sterilization regulator of chitosan oligosaccharide and S-abscisic acid as claimed in claim 1, which is characterized by comprising the following steps:
(1) preparing S-abscisic acid: performing seed culture and fermentation culture on a strain capable of fermenting to produce S-abscisic acid to obtain fermentation liquor containing the S-abscisic acid, performing plate-frame filtration, membrane filtration, acid precipitation and extraction on the fermentation liquor containing the S-abscisic acid to obtain extract liquor, and performing concentration, crystallization, recrystallization and drying on the extract liquor to obtain an S-abscisic acid finished product with the purity of more than 95%;
(2) adding chitosan oligosaccharide, S-abscisic acid, amino acid stock solution, urea, glutamic acid and an auxiliary agent into an ethanol solution for dissolving;
(3) and (3) adding the solution obtained after the dissolution in the step (2) and the S-abscisic acid prepared in the step (1) into water, and then uniformly stirring to obtain the compound sterilization regulator.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110091419.2A CN112674097A (en) | 2021-01-23 | 2021-01-23 | Chitosan oligosaccharide and S-abscisic acid compound sterilization regulator and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116058368A (en) * | 2023-03-01 | 2023-05-05 | 华植河北生物科技有限公司 | Pesticide composition containing carvacrol, preparation and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103548829A (en) * | 2013-11-15 | 2014-02-05 | 四川国光农化股份有限公司 | Plant growth regulating composition, preparation and application |
| CN107058410A (en) * | 2017-04-18 | 2017-08-18 | 四川龙蟒福生科技有限责任公司 | A kind of preparation method of S abscisic acids |
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- 2021-01-23 CN CN202110091419.2A patent/CN112674097A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103548829A (en) * | 2013-11-15 | 2014-02-05 | 四川国光农化股份有限公司 | Plant growth regulating composition, preparation and application |
| CN107058410A (en) * | 2017-04-18 | 2017-08-18 | 四川龙蟒福生科技有限责任公司 | A kind of preparation method of S abscisic acids |
Non-Patent Citations (1)
| Title |
|---|
| 郑先福: "《植物生长调节剂应用技术》", 30 June 2013, 中国农业大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116058368A (en) * | 2023-03-01 | 2023-05-05 | 华植河北生物科技有限公司 | Pesticide composition containing carvacrol, preparation and application thereof |
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Application publication date: 20210420 |