CN112501232A - Preparation method of yak bone peptide powder - Google Patents
Preparation method of yak bone peptide powder Download PDFInfo
- Publication number
- CN112501232A CN112501232A CN202011530841.5A CN202011530841A CN112501232A CN 112501232 A CN112501232 A CN 112501232A CN 202011530841 A CN202011530841 A CN 202011530841A CN 112501232 A CN112501232 A CN 112501232A
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- Prior art keywords
- peptide powder
- bone peptide
- preparation
- yak bone
- environmental protection
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Water Supply & Treatment (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses a preparation method of yak bone peptide powder, which comprises the steps of selecting fresh yak bones, cleaning, removing impurities, crushing, cooking, hydrolyzing, deoiling, performing enzymolysis on an obtained primary product by using a special complex enzyme preparation for customized food, then performing enzyme deactivation, performing solid-liquid separation, decoloring and deodorizing filtrate by using activated carbon, filtering, concentrating the filtrate, sterilizing a concentrated product, performing high-pressure spray drying, weighing, packaging, removing noise, and treating a waste packaging material to meet the requirement of environmental protection to obtain the yak bone peptide powder; the method has no noise and pollution in the whole process, just caters to the national industrial policy from the aspect of environmental protection, simultaneously improves the yield and reduces the production cost of the whole enterprise.
Description
Technical Field
The invention belongs to the technical field of health care products, and particularly relates to a preparation method of yak bone peptide powder.
Background
The skeleton and joints of a human body become more fragile with the increase of age and the loss of collagen, the yak bone peptide has high nutritional characteristics, can be used as a metabolism agent, promotes the biosynthesis of organism collagen, and can achieve the effect of preventing osteoporosis by eating the yak bone peptide.
The existing extraction process of the yak bone peptide powder is long in time consumption, the batches are difficult to unify, and the quality of the obtained product needs to be improved.
Disclosure of Invention
The invention aims to provide a preparation method of yak bone peptide powder aiming at the defects in the prior art.
The technical scheme adopted by the invention for solving the technical problems is as follows: a method for preparing yak bone peptide powder comprises the following steps
(1) Pretreatment: selecting fresh yak bones, cleaning, removing impurities, crushing, removing noise to reduce noise pollution, and then cooking and hydrolyzing;
(2) enzymolysis: after deoiling, cooling to 50-70 ℃, performing low-temperature enzymolysis on the obtained primary product for 4 hours by adopting one or more mixtures of papain, trypsin and neutral protease, then heating to the temperature of more than 100 ℃ to keep the enzymolysis liquid for 10 minutes for enzyme deactivation, and performing solid-liquid separation;
(3) concentration: decoloring the filtrate by using activated carbon, removing odor, removing excessive moisture, treating the waste activated carbon to meet the requirement of environmental protection, filtering, and concentrating the filtrate to 1/3-1/5 of the original volume;
(4) and (3) post-treatment: and sterilizing the concentrated product, performing high-pressure spray drying, removing noise and dust simultaneously to meet the environmental protection requirement, weighing, packaging, removing noise simultaneously, and treating the waste packaging material to meet the environmental protection requirement to obtain the yak bone peptide powder.
According to the preparation method of the yak bone peptide powder, the beef tallow removed in the step (2) is decolored and deodorized by using active carbon, and the beef tallow is obtained for further use after dehydration.
The preparation method of the yak bone peptide powder comprises the following steps of (2) drying the filter residue after solid-liquid separation, carrying out superfine grinding, weighing and packaging, and using the filter residue as a fertilizer for another use.
The invention has the beneficial effects that: the invention adopts the special customized compound enzyme preparation for enzymolysis, the obtained product is colorless, the dissolution is fast, the natural yak bone fragrance is not lost, the product molecular weight is smaller, the yield and the cost are obviously reduced, the social benefit is higher, the popularization is worthy, and the leftovers can be used as the fertilizer, the whole process is free from noise and pollution, and the method is consistent with the national industrial policy.
Drawings
FIG. 1 is a schematic flow diagram of the process of the present invention.
Detailed Description
The technical solution in the embodiments of the present invention is clearly and completely described below with reference to the drawings of the embodiments of the present invention.
Referring to fig. 1, the preparation method of the yak bone peptide powder disclosed by the invention comprises the following steps
(1) Pretreatment: selecting fresh yak bones, cleaning, removing impurities, crushing (in the step, noise needs to be removed, and noise pollution is reduced), and then cooking and hydrolyzing.
(2) Enzymolysis: removing oil, decolorizing and deodorizing filtrate by using activated carbon for removed beef tallow (the step needs to treat waste activated carbon to meet the requirement of environmental protection), performing enzymolysis on the obtained primary product by using a special complex enzyme preparation for customized food, cooling to 50-70 ℃, performing low-temperature enzymolysis for 4 hours by using one or a mixture of papain, trypsin and neutral protease, raising the temperature to above 100 ℃ to keep the enzymolysis liquid for 10 minutes for enzyme deactivation, performing solid-liquid separation, drying and performing superfine grinding on filter residues after the solid-liquid separation (the step needs to remove noise and reduce noise pollution), weighing and packaging, and using the filter residues as a fertilizer for additional use.
(3) Concentration: and (3) decoloring and deodorizing the filtrate by using activated carbon (the step needs to treat the waste activated carbon to meet the environmental protection requirement), so that the product has a particularly good color, filtering, concentrating the filtrate, and concentrating the filtrate to 1/3-1/5 of the original volume.
(4) And (3) post-treatment: and (3) sterilizing the concentrated product, performing high-pressure spray drying (noise and dust are removed in the step to meet the environmental protection requirement), weighing and packaging (noise is removed in the step, and the waste packaging material is treated to meet the environmental protection requirement), and thus obtaining the yak bone peptide powder.
The product obtained by the invention has smaller molecular weight, obviously improved product activity, quicker absorption by human body, no color, no loss of natural beef bone flavor, improved product yield, reduced production cost of the whole enterprise, quicker absorption of the product, obvious effect on gout people, no rebound after good treatment, and complete inclusion of the fifth item detected by an authority mechanism, and is deeply favored by consumers to obtain the product.
The above-described embodiments are merely illustrative of the principles and effects of the present invention, and some embodiments may be applied, and it will be apparent to those skilled in the art that various changes and modifications may be made without departing from the inventive concept of the present invention, and these embodiments are within the scope of the present invention.
Claims (3)
1. A preparation method of yak bone peptide powder is characterized by comprising the following steps: comprises the following steps
(1) Pretreatment: selecting fresh yak bones, cleaning, removing impurities, crushing, removing noise to reduce noise pollution, and then cooking and hydrolyzing;
(2) enzymolysis: after deoiling, cooling to 50-70 ℃, carrying out enzymolysis on the obtained primary product for 4 hours by adopting one or a mixture of papain, trypsin and neutral protease, then raising the temperature to more than 100 ℃ to keep the enzymolysis liquid for 10 minutes for enzyme deactivation, and then carrying out solid-liquid separation;
(3) concentration: decoloring and deodorizing the filtrate by using activated carbon, treating the waste activated carbon to meet the environmental protection requirement, filtering, and concentrating the filtrate to 1/3-1/5 of the original volume;
(4) and (3) post-treatment: and sterilizing the concentrated product, performing high-pressure spray drying, removing noise and dust simultaneously to meet the environmental protection requirement, weighing, packaging, removing noise simultaneously, and treating the waste packaging material to meet the environmental protection requirement to obtain the yak bone peptide powder.
2. The preparation method of the yak bone peptide powder as claimed in claim 1, wherein the beef tallow removed in the step (2) is decolorized and deodorized () by using activated carbon, and the beef tallow is obtained for further use after dehydration.
3. The preparation method of the yak bone peptide powder as claimed in claim 1, wherein the filter residue obtained after the solid-liquid separation in the step (2) is dried, subjected to superfine grinding () and then weighed and packaged for use as a fertilizer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011530841.5A CN112501232A (en) | 2020-12-22 | 2020-12-22 | Preparation method of yak bone peptide powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011530841.5A CN112501232A (en) | 2020-12-22 | 2020-12-22 | Preparation method of yak bone peptide powder |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112501232A true CN112501232A (en) | 2021-03-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011530841.5A Withdrawn CN112501232A (en) | 2020-12-22 | 2020-12-22 | Preparation method of yak bone peptide powder |
Country Status (1)
| Country | Link |
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| CN (1) | CN112501232A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114032276A (en) * | 2021-12-03 | 2022-02-11 | 神话(辽宁)新能源科技股份有限公司 | Yak bone collagen peptide powder technology |
-
2020
- 2020-12-22 CN CN202011530841.5A patent/CN112501232A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114032276A (en) * | 2021-12-03 | 2022-02-11 | 神话(辽宁)新能源科技股份有限公司 | Yak bone collagen peptide powder technology |
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| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20210316 |