CN112410216A - Method for dynamically adjusting dissolved oxygen in reactor by using nitrogen and oxygen - Google Patents
Method for dynamically adjusting dissolved oxygen in reactor by using nitrogen and oxygen Download PDFInfo
- Publication number
- CN112410216A CN112410216A CN202011128144.7A CN202011128144A CN112410216A CN 112410216 A CN112410216 A CN 112410216A CN 202011128144 A CN202011128144 A CN 202011128144A CN 112410216 A CN112410216 A CN 112410216A
- Authority
- CN
- China
- Prior art keywords
- dissolved oxygen
- reactor
- mass flow
- flow controller
- pneumatic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
Abstract
The invention relates to the technical field of cell culture, in particular to a method for dynamically adjusting the dissolved oxygen in a reactor by nitrogen and oxygen, which comprises a reactor glass tank, a peristaltic pump, a dissolved oxygen sensor fixing block, a dissolved oxygen sensor, an oxygenator, an N2 mass flow controller, an N2 pneumatic quadruple, a 02 mass flow controller, a 02 pneumatic quadruple and a silicone tube, wherein the invention designs a method for dynamically adjusting the dissolved oxygen in the reactor by nitrogen and oxygen, thereby achieving more efficient dissolved gas of an oxygenator module and quickly adjusting the dissolved oxygen value; high-precision PID closed-loop control, and the dissolved oxygen value can reach +/-1%; the stability and the safety are high, the dissolved oxygen is detected in a non-contact way, and the pollution risk is avoided; the vortex stirring of no stirring rake, the more even effect of culture solution dissolved oxygen in the glass jar to realized whole dissolved oxygen adjusting device full automatization, improved cell culture's efficiency greatly, practiced thrift the time cost.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a method for dynamically adjusting the dissolved oxygen in a reactor by using nitrogen and oxygen.
Background
In the bioreactor production process, the dissolved oxygen has a great influence on the growth of cells. Dissolved oxygen in the cell culture solution is not adjusted well, can lead to cell growth to slow down or direct massive death, and traditional bioreactor has air distribution inhomogeneous, has the stirring dead angle during stirring rake stirring culture solution for oxygen can not fully be absorbed by the culture solution, causes the defect that dissolved oxygen is not enough.
In conclusion, the invention solves the existing problems by designing a method for dynamically regulating the dissolved oxygen amount in a reactor by using nitrogen and oxygen.
Disclosure of Invention
The invention aims to provide a method for dynamically regulating the dissolved oxygen in a reactor by using nitrogen and oxygen, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a method for dynamically adjusting the dissolved oxygen in a reactor by nitrogen and oxygen comprises a reactor glass tank, a peristaltic pump, a dissolved oxygen sensor fixing block, a dissolved oxygen sensor, an oxygenator, an N2 mass flow controller, an N2 pneumatic quadruple, a 02 mass flow controller, a 02 pneumatic quadruple and a silicone tube, and comprises the following specific steps:
s1, setting the dissolved oxygen value detected by the dissolved oxygen sensor; meanwhile, the flow of N2 of the mass flow controller N2 is controlled to be constant through a PID closed loop;
s2, pumping the culture solution out of the bottom of the reactor glass tank by a peristaltic pump, passing through a dissolved oxygen sensor fixing block, passing through a liquid inlet of an oxygenator, and enabling a liquid outlet of the oxygenator to flow to the top of the reactor glass tank, so that a vortex is formed in the reactor glass tank, namely the whole loop is kept in a circulating state all the time;
s3, when the dissolved oxygen sensor detects that the dissolved oxygen value of the culture solution is higher, the mass flow controller 02 is controlled in a PID closed loop mode, and the flow is reduced by the mass flow controller 02 so as to reduce the dissolved oxygen;
and S4, when the dissolved oxygen sensor detects that the dissolved oxygen value of the culture solution is low, the mass flow controller 02 is controlled by a PID closed loop, so that the flow of the mass flow controller 02 is increased, and the dissolved oxygen is increased.
Preferably, a port of the peristaltic pump is connected with a liquid outlet of the reactor glass tank through a silicone tube, another port of the peristaltic pump is connected with a dissolved oxygen sensor fixing block through a silicone tube, and the dissolved oxygen sensor fixing block is respectively connected with the peristaltic pump and the dissolved oxygen sensor fixing block through silicone tubes.
Preferably, the oxygenator is connected to the N2 mass flow controller and the 02 mass flow controller through silicone tubes, respectively.
Preferably, the N2 mass flow controller is connected to the N2 pneumatic quadruplet through a silicone tube, and the N2 pneumatic quadruplet is connected to the N2 gas source end through a silicone tube.
Preferably, the 02 mass flow controller is connected with the 02 pneumatic quadruple through a silicone tube, and the 02 pneumatic quadruple is connected with the 02 gas source end through a silicone tube.
Preferably, the dissolved oxygen sensor is mounted on a dissolved oxygen sensor fixing block, and the peristaltic pump, the oxygenator, the N2 mass flow controller, the N2 pneumatic quadruple, the 02 mass flow controller, the 02 pneumatic quadruple and the dissolved oxygen sensor are respectively and electrically connected to the PID closed-loop controller through leads.
Compared with the prior art, the invention has the beneficial effects that:
1. in the invention, a method for dynamically adjusting the dissolved oxygen in the reactor by nitrogen and oxygen is designed, so that more efficient dissolved gas of the oxygenator module is added, and the dissolved oxygen value is quickly adjusted; high-precision PID closed-loop control, and the dissolved oxygen value can reach +/-1%; the stability and the safety are high, the dissolved oxygen is detected in a non-contact way, and the pollution risk is avoided; the vortex stirring of no stirring rake, the more even effect of culture solution dissolved oxygen in the glass jar to realized whole dissolved oxygen adjusting device full automatization, improved cell culture's efficiency greatly, practiced thrift the time cost, solved traditional bioreactor and had air distribution inhomogeneous, there is the stirring dead angle when stirring rake stirring culture solution, make oxygen can not fully be absorbed by the culture solution, cause the defect that dissolved oxygen is not enough.
Drawings
FIG. 1 is a schematic diagram of the system of the present invention.
In the figure: 1-a reactor glass tank, 2-a peristaltic pump, 3-a dissolved oxygen sensor fixing block, 4-a dissolved oxygen sensor, 5-an oxygenator, 6-N2 mass flow controller, 7-N2 pneumatic quadruple, 8-02 mass flow controller, 9-02 pneumatic quadruple and 10-a silicone tube.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
Referring to fig. 1, the present invention provides a technical solution:
a method for dynamically adjusting the dissolved oxygen in a reactor by nitrogen and oxygen comprises a reactor glass tank 1, a peristaltic pump 2, a dissolved oxygen sensor fixing block 3, a dissolved oxygen sensor 4, an oxygenator 5, an N2 mass flow controller 6, an N2 pneumatic quadruplet 7, a 02 mass flow controller 8, a 02 pneumatic quadruplet 9 and a silicone tube 10, and comprises the following specific steps:
s1, setting the dissolved oxygen value detected by the dissolved oxygen sensor 4; meanwhile, the flow of N2 of the N2 mass flow controller 6 is controlled to be constant through a PID closed loop;
s2, pumping out the culture solution from the bottom of the reactor glass tank 1 by the peristaltic pump 2, passing through the dissolved oxygen sensor fixing block 3, passing through the liquid inlet of the oxygenator 5, and flowing the liquid outlet of the oxygenator 5 to the top of the reactor glass tank 1, so that a vortex is formed in the reactor glass tank 1, namely the whole loop is kept in a circulating state all the time;
s3, when the dissolved oxygen sensor 3 detects that the dissolved oxygen value of the culture solution is higher, the mass flow controller 02 is controlled by a PID closed loop, and the flow is reduced by 02 so as to reduce the dissolved oxygen;
s4, when the dissolved oxygen sensor 3 detects that the dissolved oxygen value of the culture solution is low, the mass flow controller 02 is controlled by the PID closed loop, so that the flow of the mass flow controller 02 is increased, and the dissolved oxygen is increased.
The specific implementation case is as follows:
referring to fig. 1, a method for dynamically adjusting the dissolved oxygen in a reactor by nitrogen and oxygen comprises a reactor glass tank 1, a peristaltic pump 2, a dissolved oxygen sensor fixing block 3, a dissolved oxygen sensor 4, an oxygenator 5, an N2 mass flow controller 6, an N2 pneumatic quadruple 7, a 02 mass flow controller 8, a 02 pneumatic quadruple 9 and a silicone tube 10, preferably, one port of the peristaltic pump 2 is connected to a liquid outlet of the reactor glass tank 1 through the silicone tube 10, the other port of the peristaltic pump 2 is connected to the dissolved oxygen sensor fixing block 3 through the silicone tube 10, the dissolved oxygen sensor fixing block 3 is respectively connected to the peristaltic pump 2 and the dissolved oxygen sensor fixing block 3 through the silicone tube 10, the oxygenator 5 is respectively connected to the N2 mass flow controller 6 and the 02 mass flow controller 8 through the silicone tube 10, the N2 mass flow controller 6 is connected to the N2 pneumatic quadruple 7 through the silicone tube 10, the pneumatic quadruple 7 of N2 passes through silicone tube 10 and connects the gas source end at N2, 02 pneumatic mass flow controller 8 passes through silicone tube 10 and connects at 02 pneumatic quadruple 9, 02 pneumatic quadruple 9 passes through silicone tube 10 and connects the gas source end at 02, dissolved oxygen sensor 4 installs on dissolved oxygen sensor fixed block 3, peristaltic pump 2, oxygenator 5, N2 mass flow controller 6, the pneumatic quadruple 7 of N2, 02 mass flow controller 8, the pneumatic quadruple 9 of 02 and dissolved oxygen sensor 4 pass through wire electric connection respectively on PID closed-loop controller, its concrete step is as follows:
step 1, setting a dissolved oxygen value detected by a dissolved oxygen sensor 4; meanwhile, the flow of N2 of the N2 mass flow controller 6 is controlled to be constant through a PID closed loop;
step 2, pumping out the culture solution from the bottom of the reactor glass tank 1 by a peristaltic pump 2, passing through a dissolved oxygen sensor fixing block 3, passing through a liquid inlet of an oxygenator 5, and flowing a liquid outlet of the oxygenator 5 to the top of the reactor glass tank 1, so that a vortex is formed in the reactor glass tank 1, namely the whole loop is kept in a circulating state all the time;
step 4, when the dissolved oxygen sensor 3 detects that the dissolved oxygen value of the culture solution is low, the mass flow controller 02 is controlled in a PID closed loop mode, the flow is increased by 02, so that the dissolved oxygen is increased, the dissolved oxygen of the cell culture solution of the reactor is detected in real time by the dissolved oxygen sensor in the cell culture process, and when the dissolved oxygen is larger than a set value, the flow of N2 is unchanged, and the flow of 02 is reduced to reduce the dissolved oxygen value; when the dissolved oxygen is less than the set value, adopting the constant flow of N2, improving the flow of 02 to improve the dissolved oxygen value, and adopting the method for dynamically adjusting the dissolved oxygen in the reactor by nitrogen and oxygen as follows:
1) the PID closed loop circuit ensures reliable flow control, and the dissolved oxygen value is adjusted through the flow of 02 and N2;
2) a pneumatic quadruple is added to filter gas, so that dust impurities and bacterial pollution in the gas are avoided;
3) the dissolved oxygen value is measured in a non-direct contact manner, a gas loop and a liquid loop do not need to be cut off, pollution is avoided, and the efficiency is improved;
4) the oxygenator module is added, so that the efficiency of gas dissolved in liquid is higher, and the dissolved oxygen value is quickly adjusted;
5) the peristaltic pump drives the circulation of the culture solution to form a vortex in the glass tank of the reactor, the vortex stirring without the stirring paddle ensures that the dissolved oxygen of the culture solution in the glass tank is more uniform, so that a method for dynamically regulating the dissolved oxygen in the reactor by nitrogen and oxygen is designed in the process, thereby achieving more efficient dissolved gas addition of the oxygenator module and fast regulation of the dissolved oxygen value; high-precision PID closed-loop control, and the dissolved oxygen value can reach +/-1%; the stability and the safety are high, the dissolved oxygen is detected in a non-contact way, and the pollution risk is avoided; the vortex stirring of no stirring rake, the more even effect of culture solution dissolved oxygen in the glass jar to realized whole dissolved oxygen adjusting device full automatization, improved cell culture's efficiency greatly, practiced thrift the time cost.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. The method for dynamically adjusting the dissolved oxygen in the reactor by using nitrogen and oxygen comprises a reactor glass tank (1), a peristaltic pump (2), a dissolved oxygen sensor fixing block (3), a dissolved oxygen sensor (4), an oxygenator (5), an N2 mass flow controller (6), an N2 pneumatic quadruple (7), a 02 mass flow controller (8), a 02 pneumatic quadruple (9) and a silicone tube (10), and specifically comprises the following steps:
s1, setting the dissolved oxygen value detected by the dissolved oxygen sensor (4); meanwhile, the flow of N2 of the N2 mass flow controller (6) is controlled to be constant through PID closed loop;
s2, pumping out the culture solution from the bottom of the reactor glass tank (1) by the peristaltic pump (2), passing through the dissolved oxygen sensor fixing block (3), passing through the liquid inlet of the oxygenator (5), and then flowing the liquid outlet of the oxygenator (5) to the top of the reactor glass tank (1), so that a vortex is formed in the reactor glass tank (1), namely the whole loop is kept in a circulating state all the time;
s3, when the dissolved oxygen sensor (3) detects that the dissolved oxygen value of the culture solution is higher, the mass flow controller (8) of 02 is controlled by a PID closed loop, so that the flow rate is reduced, and the dissolved oxygen is reduced;
and S4, when the dissolved oxygen sensor (3) detects that the dissolved oxygen value of the culture solution is lower, the mass flow controller 02 is closed-loop controlled by PID to increase the 02 flow so as to increase the dissolved oxygen.
2. The method for dynamically regulating the dissolved oxygen in a reactor by nitrogen and oxygen as claimed in claim 1, wherein: a port of the peristaltic pump (2) is connected with a liquid outlet of the reactor glass tank (1) through a silicone tube (10), another port of the peristaltic pump (2) is connected with a dissolved oxygen sensor fixing block (3) through the silicone tube (10), and the dissolved oxygen sensor fixing block (3) is connected with the peristaltic pump (2) and the dissolved oxygen sensor fixing block (3) through the silicone tube (10) respectively.
3. The method for dynamically regulating the dissolved oxygen in a reactor by nitrogen and oxygen as claimed in claim 1, wherein: the oxygenator (5) is respectively connected with the N2 mass flow controller (6) and the 02 mass flow controller (8) through a silicone tube (10).
4. The method for dynamically regulating the dissolved oxygen in a reactor by nitrogen and oxygen as claimed in claim 1, wherein: the N2 mass flow controller (6) is connected with the N2 pneumatic quadruple (7) through a silicone tube (10), and the N2 pneumatic quadruple (7) is connected with the gas source end of the N2 through the silicone tube (10).
5. The method for dynamically regulating the dissolved oxygen in a reactor by nitrogen and oxygen as claimed in claim 1, wherein: the 02 mass flow controller (8) is connected to the 02 pneumatic quadruple (9) through a silicone tube (10), and the 02 pneumatic quadruple (9) is connected to the 02 gas source end through the silicone tube (10).
6. The method for dynamically regulating the dissolved oxygen in a reactor by nitrogen and oxygen as claimed in claim 1, wherein: the dissolved oxygen sensor (4) is arranged on the dissolved oxygen sensor fixing block (3), and the peristaltic pump (2), the oxygenator (5), the N2 mass flow controller (6), the N2 pneumatic quadruple (7), the 02 mass flow controller (8), the 02 pneumatic quadruple (9) and the dissolved oxygen sensor (4) are respectively and electrically connected to the PID closed-loop controller through leads.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011128144.7A CN112410216A (en) | 2020-10-21 | 2020-10-21 | Method for dynamically adjusting dissolved oxygen in reactor by using nitrogen and oxygen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011128144.7A CN112410216A (en) | 2020-10-21 | 2020-10-21 | Method for dynamically adjusting dissolved oxygen in reactor by using nitrogen and oxygen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112410216A true CN112410216A (en) | 2021-02-26 |
Family
ID=74840375
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011128144.7A Pending CN112410216A (en) | 2020-10-21 | 2020-10-21 | Method for dynamically adjusting dissolved oxygen in reactor by using nitrogen and oxygen |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112410216A (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4824563A (en) * | 1985-12-04 | 1989-04-25 | Kabushiki Kaisha Meidensha | Equipment for treating waste water |
| US20090269812A1 (en) * | 2006-02-24 | 2009-10-29 | Toray Industries, Inc , A Corporation Of Japan | Method of producing chemical product and continuous fermentation apparatus |
| CN101985600A (en) * | 2010-11-09 | 2011-03-16 | 北京理工大学 | Turnover membrane bioreactor for use in micro-gravity environment |
| CN203274716U (en) * | 2013-03-14 | 2013-11-06 | 重庆绿色智能技术研究院 | Device for measuring thickness of aerobic layer and anaerobic layer of biological film |
| US20140311416A1 (en) * | 2013-03-15 | 2014-10-23 | Robert W. Stiles, Jr. | Dissolved Oxygen Control System for Aquaculture |
| CN105695321A (en) * | 2016-01-29 | 2016-06-22 | 北京交通大学 | Device and method for cultivating and domesticating anaerobic bacteria by virtue of dissolved oxygen intelligent control |
| CN105786059A (en) * | 2016-04-07 | 2016-07-20 | 西安石油大学 | Method for quantitative control of oxygen content in water and flow |
| CN205990403U (en) * | 2016-07-26 | 2017-03-01 | 平山县润众生态农业综合开发有限公司 | A kind of novel edible bacteria liquid spawn fermentation tank |
| CN109444218A (en) * | 2018-12-17 | 2019-03-08 | 西南科技大学 | Improved ambient condition simulator and application method in a kind of reaction of oxidation kinetics |
| CN110358683A (en) * | 2019-07-25 | 2019-10-22 | 中国科学院电工研究所 | A kind of bioreactor automatic control device |
-
2020
- 2020-10-21 CN CN202011128144.7A patent/CN112410216A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4824563A (en) * | 1985-12-04 | 1989-04-25 | Kabushiki Kaisha Meidensha | Equipment for treating waste water |
| US20090269812A1 (en) * | 2006-02-24 | 2009-10-29 | Toray Industries, Inc , A Corporation Of Japan | Method of producing chemical product and continuous fermentation apparatus |
| CN101985600A (en) * | 2010-11-09 | 2011-03-16 | 北京理工大学 | Turnover membrane bioreactor for use in micro-gravity environment |
| CN203274716U (en) * | 2013-03-14 | 2013-11-06 | 重庆绿色智能技术研究院 | Device for measuring thickness of aerobic layer and anaerobic layer of biological film |
| US20140311416A1 (en) * | 2013-03-15 | 2014-10-23 | Robert W. Stiles, Jr. | Dissolved Oxygen Control System for Aquaculture |
| CN105695321A (en) * | 2016-01-29 | 2016-06-22 | 北京交通大学 | Device and method for cultivating and domesticating anaerobic bacteria by virtue of dissolved oxygen intelligent control |
| CN105786059A (en) * | 2016-04-07 | 2016-07-20 | 西安石油大学 | Method for quantitative control of oxygen content in water and flow |
| CN205990403U (en) * | 2016-07-26 | 2017-03-01 | 平山县润众生态农业综合开发有限公司 | A kind of novel edible bacteria liquid spawn fermentation tank |
| CN109444218A (en) * | 2018-12-17 | 2019-03-08 | 西南科技大学 | Improved ambient condition simulator and application method in a kind of reaction of oxidation kinetics |
| CN110358683A (en) * | 2019-07-25 | 2019-10-22 | 中国科学院电工研究所 | A kind of bioreactor automatic control device |
Non-Patent Citations (1)
| Title |
|---|
| 董宇玮等: "纯氧曝气用于污泥高温好氧消化的研究", 《中国给水排水》, vol. 25, no. 09, pages 37 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2021213102A1 (en) | Multi-channel alkaline hydrogen production system | |
| CN102491446B (en) | Processing technology and device of optical fiber plasma liquid | |
| CN112410216A (en) | Method for dynamically adjusting dissolved oxygen in reactor by using nitrogen and oxygen | |
| CN112941540B (en) | Vanadium electrolyte production system and production method | |
| CN114606523B (en) | Variable-power hydrogen production system and automatic control system and method thereof | |
| CN103088091A (en) | High-efficiency and energy-saving validamycin fermenting method | |
| CN205690081U (en) | Use some voltage stabilizing feedwaies more | |
| CN112410215A (en) | Method for dynamically adjusting pH value in reactor by using nitrogen and carbon dioxide | |
| CN107021543A (en) | A kind of Solar use method for integrating concentrating photovoltaic power generation and photocatalysis Water warfare | |
| CN205275589U (en) | Photosynthetic response ware | |
| CN101574837A (en) | Device for controlling temperature of water tank of extruding machine | |
| CN114230002A (en) | Intelligent SBR sewage treatment device and method capable of automatically operating | |
| CN215713029U (en) | Air compressor machine for bacterial fermentation jar | |
| CN115692785B (en) | Stable hydrogen supply device for fuel cell and pressure stability control method for buffer tank | |
| CN112430540A (en) | Compact reactor capable of being placed in biological incubator | |
| CN218202323U (en) | Intelligent industrial wastewater treatment device | |
| CN205152025U (en) | Gas circulation formula of ventilating a little methane fermentation device | |
| CN220918656U (en) | Production system for producing hydrochloric acid by chlorinated paraffin byproduct hydrogen chloride | |
| CN202201904U (en) | Dissolved oxygen control system in high cell density fermentation process | |
| CN214360506U (en) | Denitrification filter tank capable of realizing oxygen-carrying-free water inlet automatic control balance control | |
| CN213977724U (en) | Photosynthetic biological reaction device and photosynthetic biological reaction system | |
| CN218917359U (en) | Closed circulating water multidimensional water quality monitoring device of particle accelerator | |
| CN116874077B (en) | Integrated short-cut nitrification-anaerobic ammoxidation reaction system and reaction process | |
| CN217265568U (en) | Crude oil stabilizer control system | |
| CN220926461U (en) | Novel anaerobic ammonia oxidation granular sludge device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |