CN112335654B - Composition and attractant for attracting red-neck common-room hornets, and preparation and application thereof - Google Patents
Composition and attractant for attracting red-neck common-room hornets, and preparation and application thereof Download PDFInfo
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- CN112335654B CN112335654B CN201910729888.5A CN201910729888A CN112335654B CN 112335654 B CN112335654 B CN 112335654B CN 201910729888 A CN201910729888 A CN 201910729888A CN 112335654 B CN112335654 B CN 112335654B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Insects & Arthropods (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of biological control, and particularly discloses a composition for attracting red-neck normal-room braconid bees, an attractant, and preparation and application thereof. The invention discloses a composition for inducing red-neck common-room braconid bees, which comprises: (E) - β -ocimene and nerol in a volume ratio of (E) - β -ocimene to nerol of (3-5): 1. the invention also discloses a red-neck common-room cocoon bee attractant which comprises the composition for attracting the red-neck common-room cocoon bee and a slow-release agent, wherein the volume ratio of the composition to the slow-release agent is 1 (10-50). The invention also discloses a preparation method of the red-neck common-room hornet attractant and application of the red-neck common-room hornet attractant in breeding. The method reduces the cost and improves the luring and nursing effects on the natural enemy parasitic wasp of lygus lucorum, namely the red-neck ever-room braconid wasp.
Description
Technical Field
The invention relates to the technical field of biological control. In particular to a composition and an attractant for attracting red-neck common-room braconid bees, and preparation and application thereof.
Background
Apolygus lucorum is an important pest on various economic crops such as cotton, jujube trees, grapes and the like. The degree of danger of cotton in Yangtze river basin, yellow river basin and northwest inland cotton area is increased year by year. The adult and nymphs of the green plant bugs can prick and damage the top buds, tender leaves, buds and flowers of host plants, fruits (or bolls) and other tissues, and cause damage symptoms such as rotten leaves, side branch clusters, drop or atrophy of young buds, deformation of flowers and fruits and the like. Has great influence on the yield and the quality of crops and causes great economic loss. The traditional chemical control can cause the problems of improvement of the resistance level of field pests, pesticide residue harm, environmental pollution and the like.
The red neck common room braconid Peristenus spretus is an important parasitic natural enemy of the lygus lucorum nymphs, prefers to parasitize the 2-4-year-old lygus lucorum nymphs, and has strong parasitism capability and good prevention and control effect. The method for controlling the damage of the lygus lucorum by using the natural enemy is the most economic, simple and effective treatment method at present. In recent years, a new technology for developing green prevention and control of target pests by using an insect behavior regulator is more widely applied. Compared with the traditional chemical pesticide, the technology is an environment-friendly plant protection strategy. Particularly, the natural enemy attracting and breeding agent can effectively attract natural enemy insects to effectively prevent and control target pests, and forms a 'push-pull' technical strategy by combining with measures such as pest repellents and the like, and is used for comprehensive treatment of agricultural pests.
At present, no design and application of the red-neck normal-room braconid wasp luring and breeding agent exists, so that the field application of the parasitic wasp is severely limited, and the prevention and control effect is influenced. In view of the above, it is an urgent need to solve the above problems by designing a composition for attracting red-neck normal-room braconid bees and an attracting and maintaining agent for red-neck normal-room braconid bees, so as to improve the attracting and maintaining effects on natural enemies.
Disclosure of Invention
In order to solve the problems in the prior art, the invention aims to provide a composition for attracting red-neck common-room braconid bees, which has simple components and remarkable effect.
The invention also aims to provide the luring (nursing) agent for the red-neck normal-room braconid bees, which is simple and convenient to prepare, is cheap and has long lasting period, and the preparation method thereof.
Still another object of the present invention is to provide a use of the attractant for red-neck common-room hornet in nursing.
In order to realize the purpose of the invention, the technical scheme of the invention is as follows:
a composition for attracting red-necked normal-room braconid bees, the composition comprising: (E) - β -ocimene and nerol in a volume ratio of (E) - β -ocimene to nerol of (3-5): 1, preferably 5: 1.
the invention finds that a certain attracting effect can be realized by singly adopting (E) -beta-ocimene or singly adopting nerol, but when the (E) -beta-ocimene and the nerol are compounded in a specific proportion, a synergistic effect can be realized, and an unexpected attracting effect on the red-neck normal-room calluses is obtained.
The inventor finds that the compound composition with the larger addition proportion of the (E) -beta-ocimene has better attraction effect, and when the volume ratio of the (E) -beta-ocimene to nerol reaches 10:1, the ideal attraction effect cannot be generated.
The invention also provides an attractant (a composite attracting and breeding agent) for the red-neck common-room braconid bees, wherein the attractant comprises the composition for attracting the red-neck common-room braconid bees and a slow-release agent, and the volume ratio of the composition to the slow-release agent is 1 (10-50).
The invention enables the effective components of the attractant to achieve good slow release effect by constructing a proper slow release system, and is beneficial to practical application.
In one embodiment of the invention, the volume ratio of the composition to the sustained release agent is 1: 10.
In one embodiment of the present invention, the slow release agent is liquid paraffin oil.
The invention also provides a method for preparing the attractant for the red-neck common-room braconid bees, which comprises the steps of adding the composition for attracting the red-neck common-room braconid bees into the slow-release agent, and fully and uniformly mixing.
The invention further provides application of the attractant for the red-neck common-room hornet in breeding.
In one embodiment of the invention, the application comprises adding the red-neck common-room braconid bee attractant into an attractant core as a slow-release device.
In an embodiment of the present invention, the material of the lure is one of natural rubber, synthetic rubber, polyethylene, polyvinyl chloride, paper material, resin or adhesive material.
According to the invention, the attractant core for attracting and nursing the red-neck common-room braconid bees is added into the attractant core, the attractant core is utilized as a slow-release device and slowly releases an active compound, and any natural rubber, synthetic rubber, polyethylene, polyvinyl chloride, paper materials, resin or adhesive materials can be made into the attractant core.
In one embodiment of the invention, a 2mL black polyethylene slow release vial was used as the wicking. The complex attracting and nursing agent is filled into a 2mL polyethylene slow-release bottle. Each bottle contains 1mL of the inducing and breeding agent stock solution, the bottle mouth is sealed, and the bottle is placed in a refrigerator at the temperature of minus 20 ℃ for standby.
The invention has the beneficial effects that:
according to the invention, by reasonably proportioning the (E) -beta-ocimene and nerol, the inducing and nursing effect on the natural enemy parasitic wasp of lygus lucorum, namely red neck common room braconid wasp, is greatly improved while the cost is reduced. The preparation and use methods of the composite attracting and conservation agent for red-neck evergreen braconid bees are simple and convenient, low in cost, obvious in effect and long in lasting period, the attracting and conservation efficiency of the red-neck evergreen braconid bees is improved, and the application effect of biologically preventing and controlling lygus lucorum is further improved.
Drawings
Fig. 1 is a graph showing the change of the volatilization amounts of (E) - β -ocimene and nerol in the attractant component of experimental example 2 in an outdoor natural environment.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. The examples are given without reference to the particular techniques or conditions, according to the techniques or conditions described in the literature of the art, or according to the product specifications. The reagents or instruments used are conventional products available from regular distributors, not indicated by the manufacturer.
The starting materials used in the present invention are commercially available or may be prepared by methods conventional in the art.
On the basis of the common knowledge in the field, the preferred conditions can be combined with each other, and the obtained preferred mixture ratio is within the scope of the invention as claimed.
Wherein, the red-neck normal-room braconid bees to be tested are provided by a scientific observation and experiment station of crop pests in a corridor of agricultural department, the indoor feeding conditions are that the temperature is 25 +/-2 ℃, the humidity is 60 +/-5%, the L: D is 14:10(h), and the eclosion adult bees are fed with 10% of honey water.
2mL of polyethylene slow release vials, (E) -beta-ocimene, nerol, methyl salicylate, linalool, butyl propionate, butyl butyrate, butyl acrylate and liquid paraffin oil were purchased from commercial regular research reagents companies, and were conventional products available from regular vendors.
Example 1
Mixing (E) -beta-ocimene and nerol in a volume ratio of 3:1 to prepare the composition (composite attractant composition) for attracting the red-neck common-room braconid bees.
The composition is added into liquid paraffin oil to prepare the attractant for the red-neck common-room horned beetles, and the volume ratio of the composition to the liquid paraffin oil is 1: 10.
Example 2
Mixing (E) -beta-ocimene and nerol in a volume ratio of 5:1 to prepare the composition (composite attractant composition) for attracting the red-neck common-room braconid bees.
The composition is added into liquid paraffin oil to prepare the attractant for the red-neck common-room horned beetles, and the volume ratio of the composition to the liquid paraffin oil is 1: 10.
Sucking 1.0mL of the attractant, placing the attractant into a polyethylene slow-release small bottle (lure core) and sealing to prepare the slow-release device.
Example 3
The composition ((E) -beta-ocimene and nerol) for attracting the red-neck-common-room braconid wasps prepared in the example 2 is diluted by liquid paraffin oil according to the volume ratio of 5:1 and the volume ratio of the liquid paraffin oil is 1:25 (namely, the composition is added into the liquid paraffin oil to be uniformly mixed) to prepare the red-neck-common-room braconid wasp attractant, 1.0mL of the attractant is sucked and placed in a polyethylene slow-release bottle (a lure core) to be sealed, and the slow-release device is prepared.
Example 4
The composition for attracting the red-neck and common-room braconid wasps prepared in the example 2 is diluted by liquid paraffin oil according to the volume ratio of 1:50 to prepare the red-neck and common-room braconid wasp attractant, 1.0mL of the attractant is absorbed and placed in a polyethylene slow-release bottle (attracting core) to be sealed, and the slow-release device is prepared.
Comparative examples 1 to 5
Respectively mixing (E) -beta-ocimene and nerol in a volume ratio of: mixing the components at a ratio of 1:1, 10:1, 1:3, 1:5 and 1:10 to obtain a composition (composite attraction composition), and mixing the prepared composition and liquid paraffin oil at a volume ratio of 1:10 to obtain the attractant.
Comparative examples 6 to 12
The attractant is prepared by respectively proportioning (E) -beta-ocimene, nerol, methyl salicylate, linalool, butyl propionate, butyl butyrate and butyl acrylate with liquid paraffin oil according to the volume ratio of 1: 10.
Comparative example 13
The composition for attracting the red-neck and common-room braconid wasps prepared in the example 2 is diluted by liquid paraffin oil according to the volume ratio of 1:75 to prepare the red-neck and common-room braconid wasp attractant, 1.0mL of the attractant is absorbed and placed in a polyethylene slow-release bottle (attracting core) to be sealed, and the slow-release device is prepared.
Experimental example 1
The attractants prepared in examples 1 to 2 and comparative examples 1 to 12 were respectively subjected to an indoor attracting effect test to test the attracting effect on red-neck common-room hornets.
The indoor attraction effect test (compound indoor screening) was performed using a Y-type insect olfactometer detection device, with different attractants (examples 1-2, comparative examples 1-12) as the odor source and liquid paraffin as the control, respectively. The inner diameter of the Y-shaped insect olfactometer is 2cm, the length of the main arm is 20cm, the lengths of the two arms are 15cm, and the included angle is 75 degrees. The atmosphere sampling instrument, the active carbon, the distilled water, the odor source device, the flowmeter and the glass Y-shaped pipe are connected in sequence by a Teflon pipe. A Pasteur dropper is inserted between two arms of the Y-shaped pipe and a Teflon rubber pipe connected with the flowmeter, and a filter paper strip (0.5cm multiplied by 4cm) dropwise added with 10 mu L of the compound is placed in the Pasteur dropper to serve as an odor source. The air flow rate was set at 300 mL/min.
Single 1-day-old adult Braconidae (parasitic wasps) with red neck and common room are put in from the opening of the main arm, and the timing is started when the parasitic wasps to be tested are positioned at the main arm 2/3. The selection time of each test is 5min, and if the test is not selected for more than 5min, the test is regarded as no response reaction; when the test parasitic wasp passes over any arm 1/3 and stays in this area for more than 5 seconds, it is recorded as a response. And (3) changing the position of the odor source after 5 parasitic wasps are tested, updating the odor source, and changing the Y-shaped pipe after 10 parasitic wasps are tested. Each insect tested was tested only once, 60 per group, and 3 groups were repeated. During testing, the indoor environment temperature and humidity are relatively constant, the temperature is 25 +/-2 ℃, and the humidity is 60 +/-5%.
The test results are shown in tables 1 and 2.
TABLE 1 trend reaction test results of red-neck common-room braconid wasps to (E) -beta-ocimene and nerol compound attractants with different proportions
Marked difference P<0.05, marked P of difference<0.01，χ2Examination of
TABLE 2 Trend response test results of red-neck common-room braconid bees to different attractants
Marked difference P<0.05，χ2Examination of
As can be seen from tables 1 and 2, the compositions of (E) - β -ocimene and nerol at a volume ratio of 3:1, 5:1, 1:1, 10:1, 1:3, 1:5, and 1:10 in combination all had an attracting effect on the red-neck normobiotic bombesia, and were significantly different from the control (P < 0.05) at a volume ratio of 3:1, 5:1, 1:1, and 10:1, compared to the control liquid paraffin oil. Wherein, when the volume ratio of the (E) -beta-ocimene to the nerol is (3-5): 1, the attractant has obvious synergistic effect, and when the volume ratio of the (E) -beta-ocimene to the nerol is 5:1 (P is less than 0.01), the attractant has the best attraction effect.
(E) The attraction effect of the-beta-ocimene and nerol on the red neck common room hornet in the room is obviously different from that of a control (P is less than 0.05). The attraction effect of methyl salicylate, linalool, butyl propionate, butyl butyrate and butyl acrylate on the red-neck normobia bombycis indoors is not remarkably different from that of a control (P is more than 0.2). The attraction effects of the (E) -beta-ocimene, nerol, methyl salicylate, linalool, butyl propionate, butyl butyrate and butyl acrylate are poorer than the attraction effects of the (E) -beta-ocimene and nerol when the (E) -beta-ocimene and the nerol are compounded in a volume ratio of (3-5): 1.
Experimental example 2
The sustained release effects of the sustained release devices prepared in examples 2 to 4 and comparative example 13 were respectively tested.
3 of each of the sealed vials (sustained release devices) prepared in examples 2 to 4 and comparative example 13 were taken (i.e., each treatment was repeated 3 times), placed in an outdoor natural environment, and after the placement, on days 1, 15, 30, 45 and 60, volatiles were collected by a dynamic headspace method for 3 hours, respectively, the adsorption column was rinsed with 300 μ L n-hexane, and the eluate was stored in a 1.5mL sample bottle in a freezer at-20 ℃ for further use, and the volatile amounts of (E) - β -ocimene and nerol were quantitatively analyzed by a gas chromatography-mass spectrometry (GC-MS) combination device, respectively, to reflect the natural degradation of the attractant.
The GC-MS test procedure was as follows:
the column was Rxi-5Sil MS (30 m.times.0.25 mm.times.0.25 μm, RESTEK, USA). Helium is used as carrier gas, the temperature of a sample inlet is 250 ℃, no flow splitting is performed, the flow rate of a column is 1mL/min, the flow rate of the sample inlet is 10mL/min, and the sample injection amount is 1 mu L.
Temperature programming: the initial temperature is 40 ℃, and the temperature is kept for 1 min; heating to 130 deg.C at 4 deg.C/min, and maintaining for 5 min; the temperature is raised to 250 ℃ at a speed of 10 ℃/min and kept for 5 min. MS working conditions: EI 70ev, the flow rate of carrier gas is 1mL/min, the temperature of an ion source is 250 ℃, the temperature of a transmission line is 250 ℃, the scanning range is 50-650m/z, and the scanning speed is 2500 amu/s.
The results of the tests (average of the test results of 3 sealed vials in each treatment group) of examples 2 to 4 and comparative example 13 are shown in fig. 1, and it can be seen from fig. 1 that the slow-release effect and the duration of the incubation agent are better than those of 1:75 when the volume ratio of the effective components ((E) -beta-ocimene and nerol) to the liquid paraffin is 1:10, 1:25 and 1: 50. When the two are mixed according to the volume ratio of 1:10, the contents of (E) -beta-ocimene and nerol detected after the two are filled into a polyethylene bottle are higher than those of other combinations, the detection value of the nerol still exceeds 50ng/ul after 45 days, and the detection value of the (E) -beta-ocimene still approaches 200ng/ul after 60 days, so that the effective components of the attractant reach good slow release effect under the proportion, and the outdoor lasting period is longer than 45 days.
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (8)
1. A composition for attracting red-necked normal-room braconid bees, the composition comprising: (E) - β -ocimene and nerol in a volume ratio of (E) - β -ocimene to nerol of 5: 1.
2. the attractant for the red-neck common-room braconid bees is characterized by comprising the composition for attracting the red-neck common-room braconid bees and the slow-release agent according to claim 1, wherein the volume ratio of the composition to the slow-release agent is 1 (10-50).
3. An attractant as claimed in claim 2 wherein the volume ratio of said composition to said slow release agent is 1: 10.
4. An attractant as claimed in claim 2 or claim 3 wherein said slow release agent is liquid paraffin oil.
5. A method for preparing the attractant for the red-neck frequent-room hornet according to any one of claims 2 to 4, wherein the method comprises the following steps: adding the composition for inducing the red-neck common-room braconid bees into the slow-release agent, and fully and uniformly mixing.
6. Use of the hornet attractant of any one of claims 2 to 4 or the hornet attractant prepared by claim 5 for breeding.
7. The use of claim 6, wherein the use comprises adding the red-neck hornworm attractant to a lure as a slow release.
8. The use of claim 7, wherein the lure is made of one of natural rubber, synthetic rubber, polyethylene, polyvinyl chloride, paper material, resin or adhesive material.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101617649A (en) * | 2009-08-11 | 2010-01-06 | 中国农业科学院植物保护研究所 | Pest-attracting plate, preparation method and application thereof |
| CN103141499A (en) * | 2013-03-27 | 2013-06-12 | 中国热带农业科学院环境与植物保护研究所 | Application of nerol in preparing banana flower thrip inducing agent |
| CN103766324A (en) * | 2014-02-21 | 2014-05-07 | 中国农业科学院植物保护研究所 | Method for biologically preventing green plant bugs by peristenus spretus in winter jujube orchard |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10774288B2 (en) * | 2013-09-18 | 2020-09-15 | The Werc Shop, LLC | Terpene-based compositions, processes, methodologies for creation and products thereby |
-
2019
- 2019-08-08 CN CN201910729888.5A patent/CN112335654B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101617649A (en) * | 2009-08-11 | 2010-01-06 | 中国农业科学院植物保护研究所 | Pest-attracting plate, preparation method and application thereof |
| CN103141499A (en) * | 2013-03-27 | 2013-06-12 | 中国热带农业科学院环境与植物保护研究所 | Application of nerol in preparing banana flower thrip inducing agent |
| CN103766324A (en) * | 2014-02-21 | 2014-05-07 | 中国农业科学院植物保护研究所 | Method for biologically preventing green plant bugs by peristenus spretus in winter jujube orchard |
Non-Patent Citations (5)
| Title |
|---|
| Chemical composition of peel and leaf essential oils of Citrus medica L. and C. limonimedica Lush.;Marie-Laure Lota et al;《Flavour Fragr. J.》;19991231;第14卷;161-166 * |
| Headspace Volatiles of Three Turkish Plants;M. Kürkcüoglu et al;《Journal of Essential Oil Research》;20101231;第22卷(第5期);389-392 * |
| Secondary metabolites profiling of Dracocephalum kotschyi Boiss atthree phenological stages using uni- and multivariate methods;Mohammad Fattahi et al;《Journal of Applied Research on Medicinal and Aromatic Plants》;20160429;177-185 * |
| The terpene synthase gene family in Gossypium hirsutum harbors a linalool synthase GhTPS12 implicated in direct defence responses against herbivores;Xin-Zheng Huang et al;《Plant Cell Environ.》;20181231;第41卷;261-274 * |
| 茶梢信息物引诱叶蝉三棒缨小蜂效应的检测;潘铖等;《生态学报》;20160630;第36卷(第12期);3785-3795 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NL2033791B1 (en) * | 2022-06-10 | 2023-12-18 | Univ Tarim | Host plant volatile of l.pratensis |
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