CN111751536A - Device and method for rapidly detecting residual quantity of abamectin B2a in soil - Google Patents
Device and method for rapidly detecting residual quantity of abamectin B2a in soil Download PDFInfo
- Publication number
- CN111751536A CN111751536A CN202010686584.8A CN202010686584A CN111751536A CN 111751536 A CN111751536 A CN 111751536A CN 202010686584 A CN202010686584 A CN 202010686584A CN 111751536 A CN111751536 A CN 111751536A
- Authority
- CN
- China
- Prior art keywords
- detection
- soil
- abamectin
- beaker
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2430/00—Assays, e.g. immunoassays or enzyme assays, involving synthetic organic compounds as analytes
- G01N2430/10—Insecticides
Abstract
The invention discloses a device and a method for rapidly detecting the residual quantity of abamectin B2a in soil, and the device comprises a pretreatment unit, a detection unit and a control unit, wherein the pretreatment unit comprises a pretreatment box body, a roller shaft for crushing a sample in a sampling bag and a beaker for extraction, the detection unit comprises a detection box body, a detection area which is positioned on one side of the detection box body and is connected with the beaker through a sample inlet pipe, a detection test paper assembly is placed in the detection area, and a collection bottle for collecting waste liquid is arranged below the detection area. The test paper is adopted to detect the sample liquid, and the plurality of detection belts are arranged, so that the quick qualitative detection and semi-quantitative detection of the residual quantity of the abamectin B2a can be realized, the detection range is large, detection personnel can be assisted to judge the polluted condition of the soil in the area, and the detection method has the advantages of convenience in detection, high detection accuracy and the like.
Description
Technical Field
The invention belongs to the technical field of soil detection, and particularly relates to a device and a method for quickly detecting residual quantity of abamectin B2a in soil.
Background
The Abamectin pesticide (Abamectin) is light yellow to white crystal powder, is odorless, has a melting point of 155-160 ℃, and a vapor pressure of 2 × 10-7Pa, relative density 1.16(21 ℃ C.). Slightly soluble in water; the solubility is very high in organic solvents such as toluene, methanol, ethanol, DMF, acetone and the like at the temperature of more than 15 ℃. The stability in aqueous solution (25 ℃) with pH5.5-7.0 is good; sensitive to strong acid and strong base; under sunlight, the structure is firstly transformed, and then the structure is yellowed and degraded. The avermectin B2 is a nerve toxin and has a unique action mechanism: the gamma-aminobutyric acid system acting on insect neuron synapses or neuromuscular synapses stimulates nerve endings to release GABA serving as a nerve transmission inhibitor, so that the GABA-gated C1-channel is prolonged to be opened, and a large amount of C1-is gushed to cause the potential of a nerve membrane to be super-increased, so that the nerve membrane is in an inhibition state, and therefore nerve impulse conduction is blocked, and insects are paralyzed, refused and killed. According to the Chinese pesticide toxicity classification standard, the abamectin belongs to a high-toxicity pesticide.
The abamectin B2a pesticide is easy to deposit in soil to pollute the environment, so that the detection of the residual abamectin B2a pesticide in the soil is very important, but most of the abamectin B2a pesticide in the prior art is detected by a chromatography instrument, the operation is completed in a laboratory, the field investigation is not facilitated, and the detection efficiency is greatly reduced.
Disclosure of Invention
Aiming at the technical problems, the invention provides a device and a method for rapidly detecting the residual quantity of abamectin B2a in soil.
The technical scheme of the invention is as follows: a device for rapidly detecting the residual quantity of abamectin B2a in soil, which comprises a pretreatment unit, a detection unit and a control unit,
the pretreatment unit comprises a pretreatment box body, a feed inlet is formed in the top of the pretreatment box body, a pair of roller shafts driven by a forward and reverse rotating motor are arranged below the feed inlet and used for extruding and crushing a sampling bag filled with a soil sample, a beaker is positioned below the roller shafts, an electric heating sleeve is sleeved outside the beaker, a liquid outlet and a liquid inlet which penetrate through the side wall of the pretreatment box body are respectively formed in two sides of the bottom of the beaker, a first electromagnetic valve and a second electromagnetic valve are respectively arranged on the liquid outlet and the liquid inlet, and a bidirectional electromagnetic stirrer is arranged below the beaker and used for driving a magnetic rotor in the beaker to rotate;
the detection unit comprises a detection box body connected below the pretreatment box body, a detection area positioned above the right side of the detection box body, a detection test paper assembly is placed in the detection area, a sample inlet pipe with a first micro metering pump is arranged at the top of the detection area, the upper end of the sample inlet pipe is connected with a liquid outlet, the lower end of the sample inlet pipe is positioned above the detection test paper assembly, a collection bottle which is positioned below the detection area and used for collecting waste liquid, and a reagent storage bottle which is positioned on the left side of the detection box body, wherein the reagent storage bottle is connected with the liquid inlet through a liquid feeding pipe with a second micro metering pump and used for quantitatively conveying a reagent for leaching abamectin B;
the control unit comprises a control box and a storage battery, wherein the control box is positioned in the middle of the detection box body, and a controller, a display screen and an operation button are arranged on the outer wall of the control box, and the controller is electrically connected with a forward and reverse rotating motor, an electric heating sleeve, a first electromagnetic valve, a second electromagnetic valve, a bidirectional electromagnetic stirrer, a first miniature metering pump and a second miniature metering pump.
Furthermore, the sampling bag is made of a nylon filter membrane with the aperture of 0.22 μm, the upper port of the sampling bag is connected with a PVC plastic sheet, the inner side of the PVC plastic sheet is provided with a sealing strip, and the upper end of the PVC plastic sheet is provided with a fixing ring for connecting a traction rope. Utilize the nylon filter membrane can prevent that soil from directly adding to be difficult to the clearance in the beaker for it is more convenient to detect.
Further, the bottom of detection zone is equipped with the funnel that lower extreme and collecting bottle link to each other, is equipped with the limiting plate that is used for holding the test paper subassembly with adding in the top of funnel, and the inspection window that runs through detection box lateral wall is seted up on the detection zone right side, and inspection window is used for the limiting plate business turn over.
Furthermore, the limiting plate is provided with a through hole for liquid leakage, a strip-shaped groove for placing the test paper assembly and communicating with the through hole, and arc-shaped grooves which are arranged on two sides of the strip-shaped groove and are convenient for taking and placing the test paper assembly. Utilize the limiting plate to fix the test paper subassembly supreme at the funnel, can guarantee that the absorbent cotton sliver of test paper subassembly front end just lies in into appearance pipe end below, can ensure to contain target liquid and can accurately drench absorbent cotton sliver, and unnecessary liquid can fall into the final inflow collecting bottle of funnel through the through-hole simultaneously, can guarantee absorbent cotton sliver and adsorb the liquid of capacity, and it is more accurate to obtain the detection.
Furthermore, the test paper assembly comprises a shell with a cover cap, test paper for detecting the abamectin B2a is fixed in the shell, a transparent plastic window convenient for observing the test paper is arranged on the surface of the shell, and index graduated scales are positioned on two sides of the transparent plastic window, and the front end of the test paper is connected with a water absorption cotton sliver extending out of the shell and used for contacting a detection sample and transmitting the detection sample to the test paper for detection.
Furthermore, the test paper is formed by overlapping five detection bands and a cellulose nitrate filter membrane of a quality control band, wherein the interval distance between the five detection bands is 0.5cm, the five detection bands are abamectin B2a monoclonal antibodies with the concentrations of 1.0 mu g/L, 2.0 mu g/L, 5.0 mu g/L, 10.0 mu g/L and 20 mu g/L in sequence, and the quality control band is a mouse anti-chicken ovalbumin antibody. The abamectin B2a monoclonal antibody is synthesized by adopting a conventional medicine antibody synthesis technology, and by arranging a plurality of detection belts, the abamectin B2a residual quantity can be detected semi-quantitatively, the detection range is large, and detection personnel can be assisted to judge the soil pollution condition in the area.
Furthermore, the reagent is composed of acetone and dichloromethane according to the volume ratio of 1:1, the cleaning agent is a mixed solution of acetone and ethanol, and the volume ratio of the acetone to the ethanol is 1: 3-5.
Further, the lower part is equipped with the containing box in the preliminary treatment box for place the detection test paper subassembly of detection usefulness, sample bag, the relevant instrument of soil sample, be used for consolidating the sealed small-size thermoplasticity machine to the sampling bag. Soil sampling related tools include shovels, mesh screens, scales, and the like.
Furthermore, a cleaning agent storage box is arranged at the bottom of the pretreatment box body, a cleaning agent is injected into the cleaning agent storage box from the feeding hole through a flushing pipe with a cleaning pump, the roller shaft, the beaker and the sample inlet pipe are sequentially flushed, and waste liquid flows into the collecting bottle after flushing. The beaker is washed, so that next sample detection can be conveniently carried out, residues are prevented from interfering the detection result, multiple continuous detections can be carried out, and the detection is convenient and fast.
Further, the sample preparation method in the sampling bag comprises the following steps: grinding and crushing the collected soil sample, sieving, and selecting soil particles with the particle size of 20-40 meshes for later use; and then mixing and stirring the soil particles and the cooked talcum powder uniformly according to the weight ratio of 10-15: 1. The calcined talcum powder has less impurities and good chemical corrosion resistance and sliding property, can increase the relative sliding property among soil particles by being mixed with a soil sample, and improves the extraction efficiency of the reagent on the soil by increasing the uniform dispersibility of the soil particles.
The invention also provides a method for detecting the residual quantity of the abamectin B2a in the soil by using the detection device, which comprises the following steps:
s1: grinding and crushing the collected soil sample, sieving, and selecting soil particles with the particle size of 20-40 meshes for later use; mixing and stirring the soil particles and the cooked talcum powder uniformly according to the weight ratio of 10-15:1, then filling the mixture into a sampling bag, sealing the sampling bag by using a sealing strip, and finally reinforcing and sealing the sampling bag by using a small thermoplastic machine;
s2: starting two forward and reverse rotating motors to drive the two roll shafts to rotate in the same phase, feeding the sampling bag along the gap of the two roll shafts, lifting the pulling rope up and down to drive the sampling bag to move between the roll shafts, fully grinding and refining the sample in the sampling bag, closing the forward and reverse rotating motors after grinding is finished, lowering and suspending the sampling bag into a beaker, and fixing the pulling rope on a hook outside a feed port;
s3: opening a second electromagnetic valve and a second micro metering pump, conveying the reagent in a reagent storage bottle into a beaker through a liquid conveying pipe, wherein the reagent accounts for 50-60% of the volume of the beaker, closing the second electromagnetic valve and the second micro metering pump, opening an electric heating sleeve to set the heating temperature to be 40-50 ℃, simultaneously opening a bidirectional electromagnetic stirrer to drive a magnetic rotor to stir the reagent, increasing the contact rate of the reagent and the soil sample, wherein the time is 30min, the target object is extracted into the reagent after being filtered by a sampling bag, opening a forward and reverse rotating motor to drive a roller shaft to rotate every 10 min, lifting the sampling bag upwards, extruding the soil sample in the sampling bag by utilizing the extrusion force of the roller shaft through a gap of the roller shaft, accelerating the dissolution of the target object, and taking out the sampling bag after the extraction is finished;
s4: taking out the detection test paper assembly, pulling out the cap from the head of the shell, installing the cap at the tail of the shell, fixing the shell in the strip-shaped groove of the limiting plate, extending the absorbent cotton sliver leaked from the front end of the shell into the through hole, after the installation is finished, placing the limiting plate above a funnel of a detection area from an inspection window, enabling the absorbent cotton sliver in the through hole to be positioned on the same line with the tail end of the sample inlet pipe, opening the electromagnetic valve I and the micro metering pump II to extract the target-containing liquid in the beaker, wetting the absorbent cotton sliver when the target-containing liquid flows out of the sample inlet pipe, sucking the absorbent cotton sliver to the test paper through the absorbent cotton sliver, combining the test paper with a detection band on the test paper, analyzing the content of the abamectin B2a, carrying out semi-quantitative detection on the soil, taking out the detection component on; the redundant liquid falls into a collecting bottle through a funnel;
s5: after the single detection is finished, the cleaning pump can be started to flush the cleaning agent from the cleaning agent storage box through the roller shaft of the flushing pipe aligned with the feeding hole, then the cleaning agent flows into the beaker, meanwhile, the bidirectional electromagnetic stirrer is started to continue stirring, the flushed water flows into the collecting bottle through the sampling pipe, and after the cleaning is finished, the next detection is carried out.
The invention has the beneficial effects that:
(1) according to the invention, soil is filled into a sampling bag made of a nylon filter membrane material and sealed, a target object in the soil is filtered into a reagent in a beaker through the nylon filter membrane to complete extraction, the temperature of the reagent is raised and preserved through an electric heating sleeve in the extraction process, and the reagent is stirred by magnetic stirring, so that the contact rate of the reagent and the soil is improved.
(2) The pair of roll shafts is arranged in the feeding hole, so that the soil in the sampling bag can be extruded and crushed before the sampling bag is placed in the beaker, the sampling bag can be extruded and filtered in the soaking process, the filtering of target substances in the soil is assisted, and the extraction efficiency is accelerated;
(3) according to the invention, hydrated lime and soil particles are mixed according to a certain proportion and then are placed in a sampling bag to be used as a sample, the calcined hydrated talcum powder has less impurities and good chemical corrosion resistance and sliding property, the relative sliding property among the soil particles can be increased by mixing the hydrated talcum powder with the soil sample, and the extraction efficiency of the reagent on the soil is improved by increasing the uniform dispersibility of the soil particles.
(4) The test paper is adopted to detect the sample liquid, and a plurality of detection belts are arranged, so that the quick qualitative detection and semi-quantitative detection of the residual quantity of the abamectin B2a can be realized, the detection range is large, and detection personnel can be assisted to judge the polluted condition of the soil in the area.
In a word, the detection device can realize rapid detection on the residual quantity of the abamectin B2a in the soil, can also continuously work to effectively detect different soil samples, and has the advantages of convenient detection, high detection accuracy and the like.
Drawings
FIG. 1 is a cross-sectional view of the overall construction of the present invention;
FIG. 2 is a front view of the overall structure of the present invention;
FIG. 3 is a rear elevational view of the overall construction of the present invention;
FIG. 4 is a limiting plate with a test strip assembly according to the present invention;
FIG. 5 is a front cross-sectional view of FIG. 4;
FIG. 6 is a schematic diagram of the construction of the test strip assembly of the present invention;
fig. 7 is a schematic view of the structure of the sampling bag of the present invention.
Wherein, 1-pretreatment unit, 11-pretreatment box body, 12-feed inlet, 13-positive and negative rotating motor, 14-roll shaft, 15-beaker, 16-electric heating jacket, 17-liquid outlet, 18-liquid inlet, 19-first electromagnetic valve, 110-second electromagnetic valve, 111-two-way electromagnetic stirrer, 112-magnetic rotor, 113-containing box, 114-cleaning agent storage box, 115-cleaning pump, 116-flushing pipe, 2-detection unit, 21-detection box body, 22-detection zone, 221-funnel, 222-limiting plate, 223-detection window, 224-through hole, 225-strip groove, 226-arc groove, 23-detection test paper component, 231-cap, 232-shell, 233-groove, 233-arc groove, test paper component, 234-transparent plastic window, 235-index graduated scale, 236-absorbent cotton sliver, 24-first miniature metering pump, 25-sample feeding tube, 26-collecting bottle, 27-reagent storage bottle, 28-second miniature metering pump, 29-liquid feeding tube, 3-control unit, 31-control box, 32-display screen, 33-operation button, 34-storage battery, 4-sampling bag, 41-PVC plastic sheet, 42-sealing strip, 43-pulling rope and 44-fixing ring.
Detailed Description
As shown in figure 1, figure 2 and figure 3, the device for rapidly detecting the residual quantity of the abamectin B2a in the soil comprises a pretreatment unit 1, a detection unit 2 and a control unit 3,
the pretreatment unit 1 comprises a pretreatment box body 11, a feed inlet 12 is arranged at the top of the pretreatment box body 11, a pair of roller shafts 14 driven by a forward and reverse rotating motor 13 are arranged below the feed inlet 12 and used for extruding and crushing a sampling bag 4 filled with a soil sample, a beaker 15 is positioned below the roller shafts 14, an electric heating sleeve 16 is sleeved outside the beaker 15, a liquid outlet 17 and a liquid inlet 18 penetrating through the side wall of the pretreatment box body 11 are respectively arranged at two sides of the bottom of the beaker 15, an electromagnetic valve I19 and an electromagnetic valve II 110 are respectively arranged on the liquid outlet 17 and the liquid inlet 18, and a bidirectional electromagnetic stirrer 111 is arranged below the beaker 15 and used for driving a magnetic rotor 112 in the beaker 15 to rotate; as shown in FIG. 7, the sampling bag 4 is made of a nylon filter membrane with a pore diameter of 0.22 μm, a PVC plastic sheet 41 is connected to the upper port of the sampling bag 4, a sealing strip 42 is arranged on the inner side of the PVC plastic sheet 41, and a fixing ring 44 for connecting a pulling rope 43 is arranged on the upper end of the PVC plastic sheet 41. The nylon filter membrane can prevent soil from being directly added into the beaker 15 and being difficult to clean, so that the detection is more convenient. The method for preparing the sample in the sampling bag 4 comprises the following steps: grinding and crushing the collected soil sample, sieving, and selecting soil particles with the particle size of 30 meshes for later use; and then mixing and stirring the soil particles and the cooked talcum powder uniformly according to the weight ratio of 12: 1. The calcined talcum powder has less impurities and good chemical corrosion resistance and sliding property, can increase the relative sliding property among soil particles by being mixed with a soil sample, and improves the extraction efficiency of the reagent on the soil by increasing the uniform dispersibility of the soil particles.
As shown in fig. 1, the lower middle portion of the pretreatment tank 11 is provided with a storage box 113 for storing a test strip assembly 23 for testing, a sampling bag 4, a tool related to soil sampling, and a small-sized thermoplastic machine for reinforcing and sealing the sampling bag 4. Soil sampling related tools include shovels, mesh screens, scales, and the like. The bottom of the pretreatment tank body 11 is provided with a cleaning agent storage tank 114, cleaning agent is injected from the feed inlet 12 into the cleaning agent storage tank 114 through a flushing pipe 116 with a cleaning pump 115, the roller shaft 14, the beaker 15 and the sampling pipe 25 are sequentially flushed, and waste liquid flows into the collecting bottle 26 after flushing. The cleaning agent is a mixed solution of acetone and ethanol, and the volume ratio of the acetone to the ethanol is 1: 3-5. The beaker 15 is washed, so that next sample detection can be conveniently carried out, residues are prevented from interfering the detection result, multiple continuous detections can be carried out, and convenience and rapidness are realized.
As shown in fig. 1, the detecting unit 2 includes a detecting box 21 connected below the pretreatment box 11, and is located the detecting area 22 above the right side of the detecting box 21, the bottom of the detecting area 22 is provided with a funnel 221 with a lower end connected with the collecting bottle 26, a limiting plate 222 used for holding the detecting test paper assembly 23 is arranged above the funnel 221, an inspection window 223 penetrating through the side wall of the detecting box 21 is arranged on the right side of the detecting area 22, and the inspection window 223 is used for the limiting plate 222 to go in and out. As shown in fig. 4 and 5, the limiting plate 222 is provided with a through hole 224 for leakage, a strip-shaped groove 225 for placing the test paper assembly 23 and communicating with the through hole 224, and arc-shaped grooves 226 disposed on two sides of the strip-shaped groove 225 for facilitating taking and placing of the test paper assembly 23. As shown in fig. 4 and 6, the test strip assembly 23 includes a housing 232 with a cap 231, a test strip 233 fixed in the housing 232 and used for detecting abamectin B2a, a transparent plastic window 234 provided on the surface of the housing 232 and facilitating observation of the test strip 233, and an index scale 235 located on both sides of the transparent plastic window 234, wherein a tampon 236 extending out of the housing 232 is connected to the front end of the test strip 233 and used for contacting a test sample and transferring the test sample to the test strip 233 for detection. Utilize limiting plate 222 to fix test paper subassembly 23 supreme at funnel 221, can guarantee that the absorbent cotton strip 236 of test paper subassembly 23 front end just is located into the terminal below of sample tube 25, can guarantee to contain target liquid and can accurately wet absorbent cotton strip 236, unnecessary liquid can fall into funnel 221 through-hole 224 and finally flow into receiving flask 26 simultaneously, can guarantee that absorbent cotton strip 236 adsorbs the liquid of sufficient, it is more accurate to detect.
The test paper 233 is formed by overlapping nitrocellulose filter membranes provided with five detection bands and a quality control band, wherein the interval distance between the five detection bands is 0.5cm, the five detection bands are abamectin B2a monoclonal antibodies with the concentrations of 1.0 mu g/L, 2.0 mu g/L, 5.0 mu g/L, 10.0 mu g/L and 20 mu g/L in sequence, and the quality control band is a mouse anti-chicken ovalbumin antibody. The abamectin B2a monoclonal antibody is synthesized by adopting a conventional medicine antibody synthesis technology, and by arranging a plurality of detection belts, the abamectin B2a residual quantity can be detected semi-quantitatively, the detection range is large, and detection personnel can be assisted to judge the soil pollution condition in the area.
As shown in fig. 1, a sample inlet pipe 25 with a first micro-metering pump 24 is arranged at the top of the detection area 22, the upper end of the sample inlet pipe 25 is connected with the liquid outlet 17, the lower end of the sample inlet pipe 25 is positioned above the detection test paper assembly 23, a collection bottle 26 for collecting waste liquid is positioned below the detection area 22, a reagent storage bottle 27 is positioned at the left side of the detection box body 21, and the reagent storage bottle 27 is connected with the liquid inlet 18 through a liquid conveying pipe 29 with a second micro-metering pump 28 and is used for conveying a reagent for leaching abamectin B2a in the soil quantitatively in the beaker 15; the reagent consists of acetone and dichloromethane according to the volume ratio of 1: 1.
The control unit 3 comprises a control box 31 and a storage battery 34 which are positioned in the middle of the detection box body 21, and a controller which is electrically connected with the forward and reverse rotation motor 13, the electric heating sleeve 16, the first electromagnetic valve 19, the second electromagnetic valve 110, the bidirectional electromagnetic stirrer 111, the first miniature metering pump 24 and the second miniature metering pump 28, a display screen 32 and an operation button 33 which are arranged on the outer wall of the control box 31 are arranged in the control box 31.
The method for detecting the residual quantity of the abamectin B2a in the soil by using the detection device comprises the following steps:
s1: grinding and crushing the collected soil sample, sieving, and selecting soil particles with the particle size of 30 meshes for later use; mixing and stirring the soil particles and the cooked talcum powder uniformly according to the weight ratio of 12:1, then filling the mixture into a sampling bag 4, sealing the sampling bag by using a sealing strip 42, and finally reinforcing and sealing the sampling bag by using a small thermoplastic machine;
s2: the two forward and reverse rotating motors 13 are started to drive the two roll shafts 14 to rotate in the opposite directions, the sampling bag 4 is fed along the gap of the two roll shafts 14, the sampling bag 4 is driven to move between the roll shafts 14 by lifting the pulling rope 43 up and down, samples in the sampling bag 4 are fully ground and refined, after grinding is finished, the forward and reverse rotating motors 13 are closed, the sampling bag 4 is placed and suspended in the beaker 15, and then the pulling rope 43 is fixed on a hook outside the feeding hole 12;
s3: opening the second electromagnetic valve 110 and the second micro metering pump 28, sending the reagent in the reagent storage bottle 27 into the beaker 15 through the liquid sending pipe 29, wherein the reagent accounts for 55% of the volume of the beaker 15, closing the second electromagnetic valve 110 and the second micro metering pump 28, opening the electric heating sleeve 16, setting the heating temperature to be 45 ℃, simultaneously opening the bidirectional electromagnetic stirrer 111 to drive the magnetic rotor 112 to stir the reagent, increasing the contact rate of the reagent and the soil sample, setting the time to be 30min, extracting the target object into the reagent after being filtered by the sampling bag 4, opening the forward and reverse rotating motor 13 to drive the roller shaft 14 to rotate every 10 min, lifting the sampling bag 4 upwards, extruding the soil sample in the sampling bag 4 through the gap of the roller shaft 14 by utilizing the extrusion force of the roller shaft 14, accelerating the dissolution of the target object, and taking out the sampling bag 4 after the extraction is finished;
s4: the test paper assembly 23 is taken out, the cap 231 is pulled out from the head of the shell 232 and then is installed at the tail of the shell 232, the shell 232 is fixed in the strip-shaped groove 225 of the limit plate 222, and the absorbent tampon 236 leaking out of the front end of the housing 232 extends into the through hole 224, and after the installation, the position-limiting plate 222 is placed over the funnel 221 of the detection zone 22 from the inspection window 223, so that the absorbent cotton strip 236 in the through hole 224 is aligned with the end of the sample inlet pipe 25, the first electromagnetic valve 19 and the second micro metering pump 28 are opened to extract the liquid containing the target substance in the beaker 15, the liquid containing the target substance is firstly wetted by the absorbent cotton strip 236 when flowing out of the sample inlet pipe 25, and is attracted to the test paper 233 by the absorbent cotton strip 236, and combined with a detection band on the test paper 233 to analyze the content of the abamectin B2a, performing semi-quantitative detection on the soil, taking out the detection test paper assembly 23 on the limiting plate 222, and checking; the excess liquid falls into the collection bottle 26 through the funnel 221;
s5: after the single detection is finished, the cleaning pump 115 can be started to flush the cleaning agent from the cleaning agent storage tank 114 through the flushing pipe 116 aligned with the roller shaft 14 of the feeding hole 12, then the cleaning agent flows into the beaker 15, meanwhile, the bidirectional electromagnetic stirrer 111 is started to continue stirring, the flushed water flows into the collecting bottle 26 through the sampling pipe 25, and after the single detection is finished, the next detection is carried out.
Examples of the experiments
The method comprises the following steps of collecting 100 parts of soil samples, mixing the soil samples with abamectin B2a with different contents, simultaneously collecting the soil samples without pollutants, respectively setting 3 experimental groups for comparison, comparing the test result of each experimental group with the laboratory chromatographic test standard, testing the accuracy, and detecting the three experimental groups by using the device disclosed by the invention, wherein the differences and the test results are shown in a table 1:
table 13 test results of the experimental groups
Claims (10)
1. A device for rapidly detecting the residual quantity of abamectin B2a in soil is characterized by comprising a pretreatment unit (1), a detection unit (2) and a control unit (3),
the pretreatment unit (1) comprises a pretreatment box body (11), a feed inlet (12) is formed in the top of the pretreatment box body (11), a pair of roller shafts (14) driven by a forward and reverse motor (13) is arranged below the feed inlet (12) and used for extruding and crushing a sampling bag (4) filled with a soil sample, a beaker (15) is located below the roller shafts (14), an electric heating sleeve (16) is sleeved outside the beaker (15), a liquid outlet (17) and a liquid inlet (18) penetrating through the side wall of the pretreatment box body (11) are respectively arranged on two sides of the bottom of the beaker (15), a first electromagnetic valve (19) and a second electromagnetic valve (110) are respectively arranged on the liquid outlet (17) and the liquid inlet (18), and a bidirectional electromagnetic stirrer (111) is arranged below the beaker (15) and used for driving a magnetic rotor (112) in the beaker (15) to rotate;
the detection unit (2) comprises a detection box body (21) connected below the pretreatment box body (11), a detection area (22) positioned above the right side of the detection box body (21), a detection test paper assembly (23) is arranged in the detection area (22), a sample inlet pipe (25) with a first miniature metering pump (24) is arranged at the top of the detection area (22), the upper end of the sample inlet pipe (25) is connected with the liquid outlet (17), the lower end of the sample inlet pipe is positioned above the detection test paper component (23), a collecting bottle (26) which is positioned below the detection area (22) and used for collecting waste liquid, a reagent storage bottle (27) which is positioned on the left side of the detection box body (21), the reagent storage bottle (27) is connected with the liquid inlet (18) through a liquid sending pipe (29) with a micro metering pump II (28), for dosing the reagent for leaching abamectin B2a in the soil into the beaker (15);
the control unit (3) comprises a control box (31) and a storage battery (34), wherein the control box (31) is located in the middle of the detection box body (21), a controller is arranged in the control box (31) and is electrically connected with the forward and reverse rotation motor (13), the electric heating sleeve (16), the first electromagnetic valve (19), the second electromagnetic valve (110), the bidirectional electromagnetic stirrer (111), the first miniature metering pump (24) and the second miniature metering pump (28), and a display screen (32) and an operation button (33) are arranged on the outer wall of the control box (31).
2. The device for rapidly detecting the residual quantity of the abamectin B2a in the soil according to claim 1, wherein the sampling bag (4) is made of a nylon filter membrane with the aperture of 0.22 μm, the upper port of the sampling bag (4) is connected with a PVC plastic sheet (41), a sealing strip (42) is arranged on the inner side of the PVC plastic sheet (41), and a fixing ring (44) for connecting a pulling rope (43) is arranged at the upper end of the PVC plastic sheet (41).
3. The device for rapidly detecting the residual quantity of abamectin B2a in soil according to claim 1, wherein a funnel (221) with the lower end connected with the collecting bottle (26) is arranged at the bottom of the detection area (22), a limit plate (222) used for holding the test paper assembly (23) is arranged above the funnel (221), an inspection window (223) penetrating through the side wall of the detection box body (21) is formed in the right side of the detection area (22), and the inspection window (223) is used for the limit plate (222) to go in and out.
4. The device for rapidly detecting the residual quantity of the abamectin B2a in the soil according to claim 3, wherein the limiting plate (222) is provided with a through hole (224) for leakage, a strip-shaped groove (225) for placing the test paper assembly (23) and communicating with the through hole (224), and arc-shaped grooves (226) which are arranged on two sides of the strip-shaped groove (225) and are convenient for taking and placing the test paper assembly (23).
5. The device for rapidly detecting the residual quantity of the abamectin B2a in the soil as claimed in claim 1, 3 or 4, wherein the test strip assembly (23) comprises a shell (232) with a cap (231), the test strip (233) for detecting the abamectin B2a is fixed in the shell (232), a transparent plastic window (234) for conveniently observing the test strip (233) is arranged on the surface of the shell (232), index scales (235) are arranged on two sides of the transparent plastic window (234), and the front end of the test strip (233) is connected with a water absorption cotton sliver (236) extending out of the shell (232) and used for contacting a detection sample and transmitting the detection sample to the test strip (233) for detection.
6. The device for rapidly detecting the residual quantity of the abamectin B2a in the soil according to claim 4, wherein the test paper (233) is formed by overlapping a cellulose nitrate filter membrane provided with five detection bands and a quality control band, the interval distance between the five detection bands is 0.5cm, the five detection bands are abamectin B2a monoclonal antibodies with the concentrations of 1.0 mu g/L, 2.0 mu g/L, 5.0 mu g/L, 10.0 mu g/L and 20 mu g/L in sequence, and the quality control band is a mouse anti-chicken ovalbumin antibody.
7. The device for rapidly detecting the residual quantity of the abamectin B2a in the soil as claimed in claim 1, wherein the reagent is composed of acetone and dichloromethane according to a volume ratio of 1:1, the cleaning agent is a mixed solution of acetone and ethanol, and the volume ratio of the acetone to the ethanol is 1: 3-5.
8. The device for rapidly detecting the residual quantity of the abamectin B2a in the soil as claimed in claim 1, wherein a storage box (113) is arranged at the middle lower part of the pretreatment box body (11) and is used for placing a detection test paper component (23) for detection, a sampling bag (4), a soil sampling related tool and a small-sized thermoplastic machine for reinforcing and sealing the sampling bag (4).
9. The device for rapidly detecting the residual quantity of abamectin B2a in soil as claimed in claim 1, wherein a cleaning agent storage tank (114) is arranged at the bottom of the pretreatment tank body (11), the cleaning agent storage tank (114) is filled with cleaning agent from the feeding hole (12) through a flushing pipe (116) with a cleaning pump (115) to sequentially flush the roll shaft (14), the beaker (15) and the sample feeding pipe (25), and waste liquid flows into the collecting bottle (26) after flushing.
10. The method for detecting the residual quantity of the abamectin B2a in the soil by using the rapid detection device of any one of claims 1 to 9 is characterized by comprising the following steps:
step 1: grinding and crushing the collected soil sample, sieving, and selecting soil particles with the particle size of 20-40 meshes for later use; then mixing and stirring the soil particles and the cooked talcum powder uniformly according to the weight ratio of 10-15:1, and then filling the mixture into a sampling bag (4) for sealing to be used as a sample;
step 2: grinding the sampling bag (4) by a roller shaft (14), suspending the sampling bag into a beaker 15, adding a reagent accounting for 55 percent of the volume of the beaker 15, wherein the reagent consists of acetone and dichloromethane according to the volume ratio of 1:1, extracting and extracting for 30min at the temperature of 40-50 ℃ and the rotating speed of 800-;
and step 3: and pumping the target-containing sample liquid to a detection area (22) and contacting the target-containing sample liquid with a detection test paper assembly (23) arranged in the detection area (22), carrying out qualitative and semi-quantitative detection on the residual amount of the abamectin B2a in the soil through test paper, and discharging redundant waste liquid into a collection bottle (26).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010686584.8A CN111751536B (en) | 2020-07-16 | 2020-07-16 | Device and method for rapidly detecting residual quantity of abamectin B2a in soil |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010686584.8A CN111751536B (en) | 2020-07-16 | 2020-07-16 | Device and method for rapidly detecting residual quantity of abamectin B2a in soil |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111751536A true CN111751536A (en) | 2020-10-09 |
| CN111751536B CN111751536B (en) | 2022-04-19 |
Family
ID=72710617
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010686584.8A Active CN111751536B (en) | 2020-07-16 | 2020-07-16 | Device and method for rapidly detecting residual quantity of abamectin B2a in soil |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111751536B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116400062A (en) * | 2023-06-08 | 2023-07-07 | 首都儿科研究所附属儿童医院 | Muscle weakness inspection device |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1187272A (en) * | 1967-03-29 | 1970-04-08 | Canadian Ind | Automatic Soil Extraction Apparatus |
| US5028543A (en) * | 1988-03-18 | 1991-07-02 | Dexsil Corporation | Method for measuring the content of halogenated organic compounds in soil samples |
| CN202383142U (en) * | 2011-11-15 | 2012-08-15 | 黑龙江八一农垦大学 | Colloidal gold test paper for detecting abamectin (AVM) residual |
| CN109342413A (en) * | 2018-11-15 | 2019-02-15 | 广东精科环境科技有限公司 | A kind of detection method and its device of heavy metal content in soil |
| CN209878727U (en) * | 2019-01-29 | 2019-12-31 | 河南省京测检测技术有限公司 | Quick detection device of pesticide residue diebi in food |
-
2020
- 2020-07-16 CN CN202010686584.8A patent/CN111751536B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1187272A (en) * | 1967-03-29 | 1970-04-08 | Canadian Ind | Automatic Soil Extraction Apparatus |
| US5028543A (en) * | 1988-03-18 | 1991-07-02 | Dexsil Corporation | Method for measuring the content of halogenated organic compounds in soil samples |
| CN202383142U (en) * | 2011-11-15 | 2012-08-15 | 黑龙江八一农垦大学 | Colloidal gold test paper for detecting abamectin (AVM) residual |
| CN109342413A (en) * | 2018-11-15 | 2019-02-15 | 广东精科环境科技有限公司 | A kind of detection method and its device of heavy metal content in soil |
| CN209878727U (en) * | 2019-01-29 | 2019-12-31 | 河南省京测检测技术有限公司 | Quick detection device of pesticide residue diebi in food |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116400062A (en) * | 2023-06-08 | 2023-07-07 | 首都儿科研究所附属儿童医院 | Muscle weakness inspection device |
| CN116400062B (en) * | 2023-06-08 | 2023-08-18 | 首都儿科研究所附属儿童医院 | Muscle weakness inspection device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111751536B (en) | 2022-04-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111751536B (en) | Device and method for rapidly detecting residual quantity of abamectin B2a in soil | |
| CN107091875A (en) | A kind of hand-held aspiration-type electrochemical detection device for automatically processing sample | |
| CN107271529A (en) | A kind of micro-total analysis system chip for the integrated detection of heavy metal ion | |
| CN212111327U (en) | Ammonia gas monitoring device | |
| CN106018045A (en) | Pipetting mechanism for sectioning and staining cells | |
| CN206114681U (en) | Flow path device and adopt oily appearance of online survey of this flow path device | |
| CN103512849B (en) | The device detected for dispersive liquid-liquid microextraction spectrum on line and detection method | |
| CN102262089A (en) | Method for quickly and quantitatively detecting pesticide residue of cyanogens-containing synthetic pyrethroids in tea | |
| CN213779983U (en) | Kit for detecting multiple steroid hormones in blood sample | |
| CN205786048U (en) | Anion synthetic detergent extractive analysis device | |
| CN1335500A (en) | Urine sedimentation detecting method | |
| CN110823821B (en) | Device and method for detecting concentration of heavy metal ions in water based on micro-fluidic chip | |
| CN212658498U (en) | Urine sampling mechanism | |
| CN207923891U (en) | A kind of preparation facilities of immune affinity column | |
| CN201133904Y (en) | Negative-pressure liquid phase chromatograph | |
| CN208902739U (en) | A kind of permanganate index analyzer | |
| CN112649577A (en) | Food safety detection method | |
| CN100561218C (en) | Elemental analyser with band stirring apparatus gas bottle | |
| CN219161985U (en) | Online continuous monitoring system for microplastic in water body | |
| CN205562243U (en) | Dispersion liquid - liquid micro -extraction external member | |
| CN209979500U (en) | Detection equipment for detecting soil pesticide residues | |
| CN213813004U (en) | Rapid filtering device for micro-plastic in water sample | |
| CN107817333A (en) | A kind of agricultural environmental protection detection means | |
| CN109633115A (en) | A kind of water quality comprehensive toxicity detection paper chip | |
| CN113092791B (en) | Device and method for rapidly detecting female hormone and progestogen of human body |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |