CN111678750A - Biological detection platform based on centrifugal separation - Google Patents
Biological detection platform based on centrifugal separation Download PDFInfo
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- CN111678750A CN111678750A CN202010331711.2A CN202010331711A CN111678750A CN 111678750 A CN111678750 A CN 111678750A CN 202010331711 A CN202010331711 A CN 202010331711A CN 111678750 A CN111678750 A CN 111678750A
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- test tube
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
- G01N2001/2866—Grinding or homogeneising
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to a biological detection platform based on centrifugal separation, which is used for detecting the growth of plants and comprises: the device comprises a shell, a first inlet, a first transmission pipeline, a grinding device, a centrifugal separation device and a detection device, wherein the first inlet is arranged at the top of the shell, the grinding device comprises a second inlet and a first grinding block, piece and second transmission pipeline are ground to the second, the first transmission pipeline of second entry connection, first grinding piece and second grinding piece are used for grinding the plant sample, centrifugal separation device includes the circulator, first telescopic shaft, the second telescopic shaft, rotary fixture, the test tube, first telescopic shaft is connected to the circulator, the second telescopic shaft sets up in first telescopic shaft top, rotary fixture sets up in the both ends of second telescopic shaft, anchor clamps centre gripping test tube, detection device includes a plurality of fourth entries and detects the chamber, the diameter of fourth entry is unanimous with the length after the second telescopic shaft shrink, the end sets up to the extension export of bifurcation export, the diameter of extension export is less than the diameter of test tube.
Description
Technical Field
The invention relates to the field of biological detection, in particular to a biological detection platform based on centrifugal separation.
Background
The plant grows mainly through accumulating the material continuously, and the material source of the plant is mainly through the transformation of photosynthesis, but the plant is not illuminated the stronger the more, the photosynthesis will be the stronger; different plants have different photosensitivity, some are pleasurable plants, and some are unhygienic plants; the stronger the photosynthetic transformation capacity of the photic plant under the condition of stronger illumination intensity; the non-photic plants are in a dark place, the conversion capacity of photosynthesis is stronger, and the photosynthesis can be carried out by illumination.
The good growth of the plants is not only the problem of sunlight, but also the air, water and the like can have great influence on the plants. At present, plant cultivation often carries out plant adjustment according to illumination, moisture, influences the microorganism etc. of vegetation through fertilizer etc. but, on these basis, need detect the plant just can the final quality that obtains the vegetation situation, now detect the plant, often judge through the outward appearance of vegetation, judge according to the vegetation height promptly, so can't detect the material situation of plant, the macroscopical detection deviation is great, can't fully reflect the vegetation situation.
Disclosure of Invention
The purpose of the invention is as follows:
aiming at the problem that plants need to be detected, the invention provides a biological detection platform based on centrifugal separation.
The technical scheme is as follows:
a centrifugal separation-based biological assay platform for growth assay of plants, comprising: a shell, a first inlet, a first transmission pipeline, a grinding device, a centrifugal separation device and a detection device, the first inlet is arranged at the top of the shell, the grinding device comprises a second inlet, a first grinding block, a second grinding block and a second conveying pipeline, the second inlet is connected with the first transmission pipeline, the first grinding block and the second grinding block are used for grinding plant samples, the centrifugal separation device comprises a rotator, a first telescopic shaft, a second telescopic shaft, a rotary clamp and a test tube, the rotator is connected with the first telescopic shaft, the second telescopic shaft is arranged at the top end of the first telescopic shaft, the rotary clamps are arranged at two ends of the second telescopic shaft and clamp the test tube, the detection device comprises a plurality of fourth inlets and a detection cavity, and the diameter of each fourth inlet is consistent with the contracted length of the second telescopic shaft.
As a preferable mode of the present invention, the second conveying pipeline is provided with a plurality of branched outlets, each branched outlet corresponds to one test tube, and the second conveying pipeline is provided with a plurality of water spraying ports for spraying water mist to the ground plant sample.
In a preferred mode of the present invention, the end of the branched outlet is provided as an extended outlet having a diameter smaller than that of the test tube.
In a preferred aspect of the present invention, the first telescopic shaft is in an extended state in an initial state, the second telescopic shaft is in a contracted state in an initial state, and the test tube is in an initial state in which a test tube port is directed upward.
In a preferred embodiment of the present invention, the test tube port of the test tube is engaged with the extension outlet of the branched outlet in the initial state.
As a preferred aspect of the present invention, when the centrifugal separation apparatus is operated, the first telescopic shaft is contracted once, the second telescopic shaft is extended, and the rotor is rotated; after the centrifugal separation device finishes working, the second telescopic shaft contracts, the first telescopic shaft contracts for the second time, and the rotary clamp rotates the test tube opening of the test tube downwards.
In a preferred aspect of the present invention, the centrifugal separator further includes a control device that controls the first telescopic shaft, the second telescopic shaft, the rotating jig, and the rotator, and the control device sets a reference angular position.
In a preferred embodiment of the present invention, the control device controls the rotator to rotate to a reference angular position when the centrifugal separation device is finished operating.
In a preferred embodiment of the present invention, a position where the rotating jig holds the test tube is a middle portion of the test tube.
As a preferable mode of the present invention, the test tube is provided with a filter screen and a cover sheet, the filter screen is disposed at a third position below a test tube opening of the test tube, and the cover sheet is used for closing the test tube when the rotating clamp rotates the test tube until the test tube opening faces downward.
The invention realizes the following beneficial effects:
plant debris is squeezed through the centrifugal action, so that cell structures in the plant debris are destroyed, and meanwhile, cell substances are separated out when the cell structures are destroyed, so that the cell substances are separated, and the detection is convenient.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the present disclosure and together with the description, serve to explain the principles of the disclosure.
FIG. 1 is a block diagram of the present invention;
FIG. 2 is a schematic view of a centrifugal separation apparatus according to the present invention;
FIG. 3 is a top view of the centrifugal separation apparatus of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
The first embodiment is as follows:
the reference figures are figures 1-3. A centrifugal separation-based biological assay platform for growth assay of plants, comprising: the device comprises a shell 1, a first inlet 2, a first transmission pipeline 3, a grinding device 4, a centrifugal separation device 5 and a detection device, wherein the first inlet 2 is arranged at the top of the shell 1, the grinding device 4 comprises a second inlet 6, a first grinding block 7, a second grinding block 14 and a second transmission pipeline 8, the second inlet 6 is connected with the first transmission pipeline 3, the first grinding block 7 and the second grinding block 14 are used for grinding plant samples, the centrifugal separation device 5 comprises a rotator 9, a first telescopic shaft 10, a second telescopic shaft 11, a rotary clamp 12 and a test tube 13, the rotator 9 is connected with the first telescopic shaft 10, the second telescopic shaft 11 is arranged at the top end of the first telescopic shaft 10, the rotary clamp 12 is arranged at two ends of the second telescopic shaft 11, the clamp clamps clamp the test tube 13, the detection device comprises a plurality of fourth inlets and a detection cavity, the diameter of the fourth inlet is the same as the contracted length of the second telescopic shaft 11.
As a preferred mode of the present invention, the second conveying pipe 8 is provided with a plurality of branched outlets, each branched outlet corresponds to one test tube 13, and the second conveying pipe 8 is provided with a plurality of water spraying ports for spraying water mist to the ground plant sample.
In a preferred mode of the present invention, the end of the branched outlet is provided as an extended outlet having a diameter smaller than that of the test tube 13.
In a preferred embodiment of the present invention, the first telescopic shaft 10 is in an extended state in an initial state, the second telescopic shaft 11 is in a contracted state in an initial state, and the test tube 13 is in an initial state with a test tube port facing upward.
In a preferred embodiment of the present invention, the test tube 13 has a test tube opening that is engaged with the elongated outlet of the branched outlet in the initial state.
As a preferred aspect of the present invention, when the centrifugal separator 5 is operated, the first telescopic shaft 10 is contracted once, the second telescopic shaft 11 is extended, and the rotator 9 is rotated; after the centrifugal separation device 5 finishes working, the second telescopic shaft 11 contracts, the first telescopic shaft 10 contracts for the second time, and the rotating clamp 12 rotates the test tube opening of the test tube 13 downward.
In a preferred embodiment of the present invention, the centrifugal separator 5 further comprises a control device for controlling the first telescopic shaft 10, the second telescopic shaft 11, the rotating jig 12, and the rotator 9, wherein a reference angular position is set.
In a preferred embodiment of the present invention, the control device controls the rotator 9 to rotate to a reference angular position when the centrifugal separator 5 is finished operating.
In a preferred embodiment of the present invention, the position where the rotating clamp 12 clamps the test tube 13 is the middle of the test tube 13.
In a preferred embodiment of the present invention, the test tube 13 is provided with a filter screen disposed at a lower third of the test tube opening of the test tube 13, and a sealing piece for sealing the test tube 13 when the rotating jig 12 rotates the test tube 13 with the test tube opening facing downward.
In the specific implementation process, a part of the leaves of the plant sample is put in from the first inlet 2, transported through the first transporting pipe 3 and enters the grinding device 4 through the second inlet 6, the rotating directions of the first rotating shaft and the second rotating shaft are opposite, and the rotating trends of the first rotating shaft and the second rotating shaft are downward, the grinding device 4 comprises a first rotating shaft, a second rotating shaft, a first grinding block 7 and a second grinding block 14, the first rotating shaft is arranged in the center of the first grinding block 7, the second rotating shaft is arranged in the center of the second grinding block 14, and the first rotating shaft and the second rotating shaft are arranged transversely, i.e. the first grinding block 7 and the second grinding block 14 are rotated transversely, therefore, the first rotating shaft and the second rotating shaft respectively drive the first grinding block 7 and the second grinding block 14 to rotate downward, so that the leaves are in the process of being squeezed and ground, can be downwards along the rotating trend of the first grinding block 7 and the second grinding block 14, so as to facilitate grinding, thereby destroying the plant leaves and the cell structures of the leaves.
The ground plant leaves enter the centrifugal separation device 5 from the grinding device 4 through the second transmission pipeline 8, in the transmission process of the second transmission pipeline 8, water mist can be sprayed at a water spraying port arranged on the second transmission pipeline 8 and is contacted with the leaves of the ground plant sample, and cell substances can be dissolved in water due to damaged cell structures of the ground plant sample, so that the ground plant leaves can have a first water dissolving process. Second transmission pipeline 8 sets up a plurality of branching export, every branching export corresponds a test tube 13, when second transmission pipeline 8 transmits, the first blade of dissolving in water can be random transmits in second transmission pipeline 8, can be random transmits away from each branching export promptly, because branching export end sets up to the extension export, the diameter that the extension exported is less than test tube 13's diameter, first telescopic shaft 10 initial state is the extension state, and the initial state of second telescopic shaft 11 is the shrink state, and test tube 13's initial state is test tube mouth up, and test tube mouth can overlap on the extension export, and consequently, the ground plant sample that transmits out in the branching export can be inside point-to-point entering test tube 13.
Before the start of the operation, the centrifugal separation device 5 is in the initial state as described above, when the operation is started, the first telescopic shaft 10 is contracted to half height, the rotary clamp 12 is rotated to rotate the test tube opening of the test tube 13 to the central position facing the centrifugal separation device 5, the second telescopic shaft 11 is extended from the contracted state to the longest state, after the first telescopic shaft 10 and the second telescopic shaft 11 are adjusted to the complete state, the rotator 9 is rotated, the rotator 9 drives the first telescopic shaft 10 to rotate, the first telescopic shaft 10 rotates to make the clamped test tube 13 influenced by a certain centrifugal effect, because the filter screen is arranged in the test tube 13, when the ground plant leaves enter the test tube 13 from the extended outlet, smaller ones of the leaf debris can be filtered from the filter screen to the bottom of the test tube 13, and meanwhile, some cell substances of the broken cells can also penetrate into the bottom of the test tube 13 from the filter screen, and some great grinding debris can be filtered by the filter screen, consequently, drive the rotatory in-process of first telescopic shaft 10 and second telescopic shaft 11 at circulator 9, great grinding debris can be by the filter screen separation, and some cell matters in the great grinding debris can be struggled away from the atress in the grinding debris under centrifugal action's condition, thereby reach test tube 13 bottom, at the rotatory in-process of test tube 13, because the resistance of centrifugal force and test tube 13 bottom, the less grinding debris of test tube 13 bottom can extrude certain cell matters, and the cell can be extruded, thereby make the cell result destroy a large amount of, thereby separate out more cell matters.
Second telescopic shaft 11 contracts to the shortest after rotatory work, rotatory test tube 13 of rotary fixture 12 is vertical downwards to the test tube mouth, and then first telescopic shaft 10 contracts once more, first telescopic shaft 10 contracts to the shortest, thereby make the test tube 13 the test tube mouth relative with the fourth entry, because the diameter of fourth entry is unanimous with the length after 11 contractions of second telescopic shaft, the circle at test tube mouth place is included in the fourth entry by the bag on the plane, consequently, aqueous solution and the grinding broken leaf in the test tube 13 can get into through the fourth entry and detect the chamber. The detection cavity is used for detecting the electrolyte in the cells by standing the aqueous solution and taking the supernatant, so that the growth condition of the plants is judged according to the content of the electrolyte, and then the environment corresponding to the plant leaves with the optimal growth condition is selected for cultivating a large number of plants.
The above embodiments are merely illustrative of the technical ideas and features of the present invention, and are intended to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the scope of the present invention. All equivalent changes or modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.
Claims (10)
1. A centrifugal separation-based biological testing platform for growth testing of plants, comprising: a shell, a first inlet, a first transmission pipeline, a grinding device, a centrifugal separation device and a detection device, the first inlet is arranged at the top of the shell, the grinding device comprises a second inlet, a first grinding block, a second grinding block and a second conveying pipeline, the second inlet is connected with the first transmission pipeline, the first grinding block and the second grinding block are used for grinding plant samples, the centrifugal separation device comprises a rotator, a first telescopic shaft, a second telescopic shaft, a rotary clamp and a test tube, the rotator is connected with the first telescopic shaft, the second telescopic shaft is arranged at the top end of the first telescopic shaft, the rotary clamps are arranged at two ends of the second telescopic shaft and clamp the test tube, the detection device comprises a plurality of fourth inlets and a detection cavity, and the diameter of each fourth inlet is consistent with the contracted length of the second telescopic shaft.
2. A centrifugal separation-based biological detection platform according to claim 1, wherein: the second conveying pipeline is provided with a plurality of branched outlets, each branched outlet corresponds to one test tube, the second conveying pipeline is provided with a plurality of water spraying ports, and the water spraying ports are used for spraying water mist to the ground plant samples.
3. A centrifugal separation-based biological assay platform according to claim 2, wherein: the tail end of the branched outlet is arranged to be an extended outlet, and the diameter of the extended outlet is smaller than that of the test tube.
4. A centrifugal separation-based biological detection platform according to claim 3, wherein: the initial state of first telescopic shaft is extension state, the initial state of second telescopic shaft is contraction state, the initial state of test tube is that the test tube mouth is up.
5. A centrifugal separation-based biological detection platform according to claim 4, wherein: in an initial state, the test tube opening of the test tube is engaged with the extension outlet of the bifurcated outlet.
6. A centrifugal separation-based biological detection platform according to claim 1, wherein: when the centrifugal separation device works, the first telescopic shaft contracts for one time, the second telescopic shaft extends, and the rotator rotates; after the centrifugal separation device finishes working, the second telescopic shaft contracts, the first telescopic shaft contracts for the second time, and the rotary clamp rotates the test tube opening of the test tube downwards.
7. A centrifugal separation-based biological detection platform according to claim 6, wherein: the centrifugal separation device further comprises a control device, the control device controls the first telescopic shaft, the second telescopic shaft, the rotating clamp and the rotator, and a reference angle position is arranged in the control device.
8. A centrifugal separation-based biological detection platform according to claim 7, wherein: when the centrifugal separation device finishes working, the control device controls the rotator to rotate to a reference angle position.
9. A centrifugal separation-based biological detection platform according to claim 1, wherein: the position of rotary fixture centre gripping the test tube is the middle part of test tube.
10. A centrifugal separation-based biological detection platform according to claim 1, wherein: the test tube is provided with filter screen and cover plate, the filter screen set up in test tube mouth below third department of test tube, the cover plate be used for rotary fixture will the test tube will when rotatory to test tube mouth down the test tube seals.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010331711.2A CN111678750A (en) | 2020-04-24 | 2020-04-24 | Biological detection platform based on centrifugal separation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010331711.2A CN111678750A (en) | 2020-04-24 | 2020-04-24 | Biological detection platform based on centrifugal separation |
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| Publication Number | Publication Date |
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| CN111678750A true CN111678750A (en) | 2020-09-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202010331711.2A Withdrawn CN111678750A (en) | 2020-04-24 | 2020-04-24 | Biological detection platform based on centrifugal separation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114436183A (en) * | 2022-04-07 | 2022-05-06 | 深圳市鹰眼在线电子科技有限公司 | Automatic cover opening and covering method and device operation method and layered identification method and device |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1842589A (en) * | 2003-06-25 | 2006-10-04 | 马克罗珀尔生物外科公司 | Systems and methods for separating and concentrating regenerative cells from tissue |
| CN103954663A (en) * | 2014-04-08 | 2014-07-30 | 同济大学 | Method for detecting cell proliferation activity by adopting cell electrode electrochemistry |
| CN105072889A (en) * | 2013-02-08 | 2015-11-18 | 罗克伍尔国际公司 | Device and method for measuring plant growth conditions |
-
2020
- 2020-04-24 CN CN202010331711.2A patent/CN111678750A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1842589A (en) * | 2003-06-25 | 2006-10-04 | 马克罗珀尔生物外科公司 | Systems and methods for separating and concentrating regenerative cells from tissue |
| CN105072889A (en) * | 2013-02-08 | 2015-11-18 | 罗克伍尔国际公司 | Device and method for measuring plant growth conditions |
| CN103954663A (en) * | 2014-04-08 | 2014-07-30 | 同济大学 | Method for detecting cell proliferation activity by adopting cell electrode electrochemistry |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114436183A (en) * | 2022-04-07 | 2022-05-06 | 深圳市鹰眼在线电子科技有限公司 | Automatic cover opening and covering method and device operation method and layered identification method and device |
| CN114436183B (en) * | 2022-04-07 | 2022-06-14 | 深圳市鹰眼在线电子科技有限公司 | Automatic cover opening and covering method and device operation method and layered identification method and device |
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Application publication date: 20200918 |
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