CN111534427A - Full-automatic detection instrument - Google Patents
Full-automatic detection instrument Download PDFInfo
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- CN111534427A CN111534427A CN202010402603.XA CN202010402603A CN111534427A CN 111534427 A CN111534427 A CN 111534427A CN 202010402603 A CN202010402603 A CN 202010402603A CN 111534427 A CN111534427 A CN 111534427A
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- 238000001514 detection method Methods 0.000 title claims abstract description 47
- 238000003756 stirring Methods 0.000 claims abstract description 35
- 238000005336 cracking Methods 0.000 claims abstract description 23
- 238000010438 heat treatment Methods 0.000 claims description 47
- 230000033001 locomotion Effects 0.000 claims description 15
- 230000009089 cytolysis Effects 0.000 claims description 9
- 230000003287 optical effect Effects 0.000 claims description 8
- 230000005540 biological transmission Effects 0.000 claims description 5
- 150000007523 nucleic acids Chemical class 0.000 abstract description 27
- 102000039446 nucleic acids Human genes 0.000 abstract description 27
- 108020004707 nucleic acids Proteins 0.000 abstract description 27
- 238000012864 cross contamination Methods 0.000 abstract description 7
- 230000003321 amplification Effects 0.000 description 20
- 238000003199 nucleic acid amplification method Methods 0.000 description 20
- 239000011324 bead Substances 0.000 description 10
- 239000003153 chemical reaction reagent Substances 0.000 description 7
- 230000000694 effects Effects 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 238000010586 diagram Methods 0.000 description 6
- 238000000605 extraction Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 3
- 238000000197 pyrolysis Methods 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
Abstract
The invention is suitable for the technical field of nucleic acid detection equipment, and provides a full-automatic detection instrument, which comprises: the device comprises an instrument shell, a mechanical framework, a power supply module, a main control module, an in-out bin card box fixing module, a cracking cavity stirring module, a pump module, a magnet module, a valve driving module, a temperature control module and a fluorescent signal acquisition module; mechanical skeleton, the fixed setting of power module is in instrument housing, host system, the fixed module of discrepancy storehouse card box, schizolysis chamber stirring module, pump module, magnet module, valve drive module, temperature control module and fluorescence signal acquisition module are fixed to be set up on mechanical skeleton, host system with go out the fixed module of storehouse card box, schizolysis chamber stirring module, pump module, magnet module, valve drive module, temperature control module and fluorescence signal acquisition module, power module are connected. The invention has the advantages of high automation degree, no need of depending on a laboratory and manual operation, convenient carrying, simple operation, quick detection and no cross contamination.
Description
Technical Field
The invention belongs to the technical field of nucleic acid detection equipment, and particularly relates to a full-automatic detection instrument.
Background
The traditional infectious disease pathogen detection based on real-time fluorescent polymerase chain reaction needs manual operation and a professional laboratory, and the full automation of nucleic acid detection cannot be realized. The current nucleic acid detection instrument generally has the condition that false positive detection results are caused by cross contamination of amplification products. The traditional nucleic acid amplification detection is also easy to cause template cross contamination and influence the judgment of a sample detection result. This in turn leads to the following disadvantages: reagent preparation, nucleic acid extraction, nucleic acid amplification and nucleic acid signal reading are separately operated in different laboratories, and basic medical units do not have such laboratories, so that the detection of such pathogen items cannot be carried out due to large investment in the construction of such laboratories; secondly, the manual operation of a professional is relied on, the operation is complicated, the time consumption is long, the detection efficiency is low, and a detection report cannot be obtained on site; thirdly, too much manual operation increases cross contamination and the risk of infection of operators; fourth, extraction reagents for detection, nucleic acid amplification reagents, and the like need to rely on cold chain transportation and cryopreservation, which is high in cost.
Disclosure of Invention
The invention provides a full-automatic detection instrument, aiming at solving the problems.
The invention is realized in this way, a full automatic detection instrument, comprising: the device comprises an instrument shell, a mechanical framework, a power supply module, a main control module, an in-out bin card box fixing module, a cracking cavity stirring module, a pump module, a magnet module, a valve driving module, a temperature control module and a fluorescent signal acquisition module;
mechanical skeleton the power module is fixed to be set up in the instrument shell, host system the storehouse card box fixed module of cominging in and going out the schizolysis chamber stirring module the pump module the magnet module the valve drive module the temperature control module and fluorescence signal acquisition module is fixed to be set up mechanical skeleton is last, host system with go in and go out storehouse card box fixed module the schizolysis chamber stirring module the pump module the magnet module the valve drive module the temperature control module and fluorescence signal acquisition module the power module is connected.
Furthermore, be equipped with a display screen, switch and data interface on the instrument shell, the display screen the switch, data interface is fixed to be set up on the instrument shell, the switch with power module electric connection.
Furthermore, the data interface is a USB interface.
Still further, the lysis chamber stirring module is located above the lysis chamber of the microfluidic cartridge, and the lysis chamber stirring module comprises: the heating cavity and the stirring rotor are connected with the guide rail through a motor and used for controlling the heating cavity to move up and down.
Still further, the pump module includes: the device comprises a first stepping motor, a push rod in transmission connection with the first stepping motor, and a first guide rail.
Still further, the magnet module includes: the second stepping motor, the magnet cavity, the magnet fixedly arranged in the magnet cavity and the second guide rail.
Still further, the temperature control module includes: the device comprises a plurality of constant temperature heating tanks, a horizontally fixed third stepping motor, a vertically fixed fourth stepping motor, a third guide rail and a fourth guide rail, wherein the third stepping motor and the third guide rail are used for controlling the front and back movement of the constant temperature heating tanks, and the fourth stepping motor and the fourth guide rail are used for controlling the up and down movement of the constant temperature heating tanks.
Still further, the plurality of thermostatic heating baths includes: first constant temperature heating bath, second constant temperature heating bath and third constant temperature heating bath, first constant temperature heating bath second constant temperature heating bath and third constant temperature heating bath sets up side by side.
Still further, the fluorescence signal acquisition module includes: the device comprises 6 groups of signal acquisition units and a fifth stepping motor, wherein the fifth stepping motor is in transmission connection with the 6 groups of signal acquisition units.
Furthermore, the 6 groups of signal acquisition units are sequentially provided with an LED, a first optical filter, a half-transmitting and half-reflecting mirror, a second optical filter and a photodiode.
Compared with the prior art, the invention has the following beneficial effects: the power module, the main control module, the inlet and outlet bin card box fixing module, the cracking cavity stirring module, the pump module, the magnet module, the valve driving module, the temperature control module and the fluorescence signal acquisition module are fixedly installed in the mechanical framework, so that the power module, the main control module, the inlet and outlet bin card box fixing module, the cracking cavity stirring module, the pump module, the magnet module, the valve driving module, the temperature control module and the fluorescence signal acquisition module are good in fixing effect, not easy to loosen or shift during working, and high in safety; the mechanical framework is arranged in the instrument shell, so that the instrument shell has good protectiveness; the invention can automatically complete sample cracking, nucleic acid extraction, nucleic acid amplification and signal detection, and finally present a detection report; the detection instrument has the advantages of high automation degree, no need of depending on a laboratory and manual operation, convenience in carrying, simplicity in operation, rapidness in detection and no cross contamination.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the fully automatic detection instrument provided by the present invention;
FIG. 2 is a schematic diagram of the internal structure of the fully automatic detection apparatus provided by the present invention;
FIG. 3 is a schematic diagram of the internal structure of the fully automatic detection apparatus according to the present invention;
FIG. 4 is a schematic structural diagram of a temperature control module provided in the present invention;
FIG. 5 is a schematic structural diagram of a stirring module of a cracking chamber provided by the present invention;
FIG. 6 is a schematic structural view of a pump module provided by the present invention;
fig. 7 is a schematic structural view of a magnet module provided by the present invention;
FIG. 8 is a schematic structural diagram of a fluorescence signal acquisition module provided by the present invention;
fig. 9 is a signal acquisition unit provided by the present invention.
Wherein, 1, the instrument shell; 11. a display screen; 12. a switch; 13. a USB interface; 2. a mechanical skeleton; 3. a main control module; 4. the inlet and outlet cartridge fixing module; 5. a pyrolysis chamber stirring module; 51. a heating cavity; 52. a stirring rotor; 53. a motor; 54. a guide rail; 6. a pump module; 61. a first stepper motor; 62. a push rod; 63. a first guide rail; 64. a fixing plate; 65. a rack plate; 66. a gear; 7. a magnet module; 71. a second stepping motor; 72. a magnet cavity 73, a magnet 74, a second guide rail 8, a valve driving module; 9. a temperature control module; 91. a first constant temperature heating tank; 92. a second constant temperature heating tank; 93. a third constant temperature heating tank; 94. a third step motor; 95. a fourth stepping motor; 96. a third guide rail; 97. a fourth guide rail; 10. a fluorescence signal acquisition module; 101. an LED; 102. a first optical filter; 103. a semi-transparent semi-reflective mirror; 104. a second optical filter; 105. a photodiode; 106. a signal acquisition unit; 107. and a fifth stepper motor.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
According to the embodiment of the invention, the power supply module, the main control module, the in-out bin card box fixing module, the cracking cavity stirring module, the pump module, the magnet module, the valve driving module, the temperature control module and the fluorescence signal acquisition module are fixedly arranged in the mechanical framework, so that the power supply module, the main control module, the in-out bin card box fixing module, the cracking cavity stirring module, the pump module, the magnet module, the valve driving module, the temperature control module and the fluorescence signal acquisition module are good in fixing effect, are not easy to loosen or shift during working and are high in safety; the mechanical framework is arranged in the instrument shell, so that the instrument shell has good protectiveness; the invention can automatically complete sample cracking, nucleic acid extraction, nucleic acid amplification and signal detection, and finally present a detection report; the detection instrument has the advantages of high automation degree, no need of depending on a laboratory and manual operation, convenience in carrying, simplicity in operation, rapidness in detection and no cross contamination.
Example one
The invention provides a full-automatic detection instrument, as shown in figures 1-3, comprising: the device comprises an instrument shell 1, a mechanical framework 2, a power supply module, a main control module 3, an in-out bin card box fixing module 4, a cracking cavity stirring module 5, a pump module 6, a magnet module 7, a valve driving module 8, a temperature control module 9 and a fluorescence signal acquisition module 10;
Wherein, instrument case 1 is used for protecting mechanical skeleton 2 and the fixed power module, host system 3, the fixed module 4 of discrepancy storehouse card box, schizolysis chamber stirring module 5, pump module 6, magnet module 7, valve drive module 8, temperature control module 9 and the fluorescence signal acquisition module 10 that sets up in mechanical skeleton 2, avoids the inner space to receive external environment's interference, and the security is high.
The power module is used for supplying power to each module of the automatic detection instrument. The main control module 3 is a circuit control system of the whole system and is used for controlling the modules to work in order and coordinately, so that the efficiency of automatic detection is improved. Go out the fixed module 4 of income storehouse card box and be used for carrying out the action of going out the storehouse or going into the storehouse, be convenient for fix the card box or take out the card box, it is automatic respond well. The cracking cavity stirring module 5 is used for providing a temperature environment for sample cracking and rotating at a high speed to scatter magnetic beads again, and the magnetic bead scattering effect is good. The pump module 6 can move up and down to further push the movement of the piston in the microfluidic card box, so that power is provided for fluid flow, power for suction is provided for upward movement, and thrust for liquid drainage is provided for downward movement. When the magnet module 7 is used for separating magnetic beads from liquid reagents, the magnet cavity is close to the cracking cavity of the microfluidic card box, the magnetic beads are separated from the liquid reagents, and when the magnetic beads need to be scattered again, the magnet cavity is far away from the cracking cavity of the microfluidic card box. The valve driving module 8 can provide a function of rotating the valve shaft on the microfluidic cartridge, when the valve needs to be opened, the valve driving module 8 rotates the valve shaft in a forward direction, and when the valve needs to be closed, the valve driving module 8 rotates the valve shaft in a reverse direction. The temperature control module 9 is used for switching under the nucleic acid amplification cavity of the microfluidic card box continuously, can provide a temperature environment required during nucleic acid amplification, and has good temperature control effect and high temperature control precision. The fluorescence signal acquisition module 10 is used for acquiring fluorescence signals during microfluidic nucleic acid amplification.
In a specific embodiment, the power module, the main control module 3, the in-and-out bin card box fixing module 4, the cracking chamber stirring module 5, the pump module 6, the magnet module 7, the valve driving module 8, the temperature control module 9 and the fluorescence signal acquisition module 10 are fixedly installed in the mechanical framework 2, so that the power module, the main control module 3, the in-and-out bin card box fixing module 4, the cracking chamber stirring module 5, the pump module 6, the magnet module 7, the valve driving module 8, the temperature control module 9 and the fluorescence signal acquisition module 10 are good in fixing effect, are not easy to loosen or deviate during working and are high in safety; the mechanical framework 2 is arranged in the instrument shell 1, so that the instrument shell is good in protection; the invention can realize the full-automatic nucleic acid detection of 'sample in and result out', has high automation degree and less manual operation of operators; the temperature control module 9 has the advantages of good temperature control effect, high temperature switching speed and the like; the signal acquisition module has the characteristics of strong multiple detection capability and high detection speed; the detection instrument has the advantages of small overall volume, light weight, portability, simple operation, quick detection, no cross contamination and high safety.
In this embodiment, as shown in fig. 1, a display screen 11, a switch 12 and a data interface are disposed on the instrument housing 1, the display screen 11, the switch 12 and the data interface are fixedly disposed on the instrument housing 1, and the switch 12 is electrically connected to the power module. The mechanical framework 2, the power supply module, the display screen 11, the switch 12 and the data interface are fixed through the instrument shell 1. The mechanical framework 2 is used for fixing other modules of the instrument; the display interface, the operator sets the parameter, analyzes the data, prints the report and controls on the display screen 11; the switch 12 is used for turning on or starting the instrument power supply; the data interface may make a copy of the data.
In this embodiment, as shown in fig. 1, the data interface is a USB interface 13, the USB interface 13 (universal serial Bus) is a serial Bus standard, and is also a technical specification of an input/output interface, and the processor and the terminal are connected by a USB data line, so that the use is convenient.
In this embodiment, as shown in fig. 2 and 5, the lysis chamber stirring module 5 is located above the lysis chamber of the microfluidic cartridge, and the lysis chamber stirring module 5 includes: a heating cavity 51 and a stirring rotor 52, wherein the heating cavity 51 and the stirring rotor 52 are connected with a guide rail 54 through a motor 53 for controlling the heating cavity 51 to move up and down. When the sample is cracked, the heating chamber moves downwards to provide a temperature environment for sample cracking, and after the cracking is completed, the heating chamber 51 moves upwards, and the heating chamber 51 is far away from the cracking chamber. When the magnetic beads need to be dispersed, the stirring rotor 52 rotates at a high speed to re-disperse the magnetic beads.
In the present embodiment, as shown in fig. 2 and 6, the pump module 6 includes: a first stepping motor 61, a push rod 62 provided on the first stepping motor 61, and a first guide rail 63. The push rod 62 can be butted with a piston in the cartridge by controlling the telescopic motion of the push rod 62 through the first stepping motor 61. The push rod 62 moves up and down to push the piston in the microfluidic card box to move, so that power is provided for fluid flow, power for suction is provided for upward movement, and thrust for liquid drainage is provided for downward movement.
Further, first step motor 61 is connected through a sliding block with first guide rail 63, the mutual spacing setting of sliding block and first guide rail 63 avoids the sliding block to pop out on first guide rail 63, and the security is high. Through fixing first guide rail 63 on a fixed plate 64 to with fixed plate 64 fixed connection in the machinery skeleton, still be equipped with a rack plate 65 on the fixed plate 64 relative first guide rail 63, be equipped with gear 66 on the output shaft of first step motor 61, gear 66 with rack plate 65 meshes the setting to first step motor 61 can utilize the effort between rack plate 65 and the gear 66 to realize the effect of up-and-down motion at the during operation. The push rod 62 is fixed below the first stepping motor 61 and is arranged parallel to the first guide rail 63, and can push the piston in the microfluidic card box to move through up-and-down movement, so that power is provided for fluid flow, power for suction is provided for upward movement, and thrust for liquid discharge is provided for downward movement.
In the present embodiment, as shown in fig. 2 and 7, the magnet module 7 includes: a second stepping motor 71, a magnet chamber 72, a magnet 73 fixedly disposed in the magnet chamber 72, and a second guide rail 74. The output end of the second stepping motor 71 is connected with the magnet cavity 72, the magnet 73 is arranged in the magnet cavity 72, the magnet cavity 72 is arranged on the second guide rail 74, and the magnet cavity 72 can be driven to move back and forth on the second guide rail 74 when the second stepping motor 71 works, so that the magnet 73 in the magnet cavity 72 is convenient to get close to or get away from the cracking cavity of the microfluidic cartridge. When the magnetic beads need to be separated from the liquid reagent, the magnet cavity is close to the cracking cavity of the microfluidic card box, the magnetic beads are separated from the liquid reagent, and when the magnetic beads need to be scattered again, the magnet cavity is far away from the cracking cavity of the microfluidic card box.
In this embodiment, as shown in fig. 3 to 4, the temperature control module 9 includes: the device comprises a plurality of constant temperature heating tanks, a horizontally fixed third stepping motor 94, a vertically fixed fourth stepping motor 95, a third guide rail 96 and a fourth guide rail 97, wherein the third stepping motor 94 and the third guide rail 96 are used for controlling the plurality of constant temperature heating tanks to move back and forth, and the fourth stepping motor 95 and the fourth guide rail 97 are used for controlling the plurality of constant temperature heating tanks to move up and down.
In the present embodiment, as shown in fig. 3 to 4, the plurality of constant temperature heating baths includes: first constant temperature heating bath 91, second constant temperature heating bath 92 and third constant temperature heating bath 93, first constant temperature heating bath 91 second constant temperature heating bath 92 and third constant temperature heating bath 93 sets up side by side. The fourth stepping motor 95 and the fourth guide rail 97 control the three constant temperature heating tanks to move up and down, so that the heating tanks are attached to the nucleic acid amplification cavity of the microfluidic card box upwards and are far away from the nucleic acid amplification cavity downwards. The third stepper motor 94 and the third guide rail 96 control the three constant temperature heating tanks to move back and forth, and the three different constant temperature tanks are switched to the lower part of the nucleic acid amplification cavity of the microfluidic card box. The temperature control module 9 can control the three constant temperature tanks to be switched continuously below the nucleic acid amplification cavity of the microfluidic card box, so as to provide a temperature environment required by nucleic acid amplification.
In this embodiment, as shown in fig. 3 and 8, the fluorescence signal collecting module 10 includes: 6 groups of signal acquisition units 106, and fifth step motor 107, fifth step motor 107 with 6 groups of signal acquisition units 106 transmission are connected. And the fifth stepping motor 107 is used for controlling 6 groups of signal acquisition units 106 to acquire fluorescence signals of the microfluidic nucleic acid amplification chamber.
Furthermore, the 6 groups of signal acquisition units 106 are uniformly distributed on the output shaft of the fifth stepping motor 107, and the positions of the 6 groups of signal acquisition units 106 are adjusted by the movement of the fifth stepping motor 107, so that the efficiency of fluorescent signal acquisition of the microfluidic nucleic acid amplification chamber is improved.
In this embodiment, as shown in fig. 3 and fig. 8 to 9, the 6 groups of signal collecting units 106 are sequentially provided with an LED101, a first optical filter 102, a half-mirror 103, a second optical filter 104, and a photodiode 105. At the node of primer extension for nucleic acid amplification, the LED excites the amplification product, and the photodiode collects the excited fluorescent signal. The 6 groups of signal acquisition units 106 are provided with LEDs with different excitation wavelengths, and the corresponding relation between the LEDs and the microfluidic nucleic acid amplification cavity can be switched by the rotation of the fifth stepping motor 107, so that fluorescent signals with different wavelengths can be acquired in a time-sharing segmented manner during nucleic acid amplification.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. A fully automatic detection instrument, comprising: the device comprises an instrument shell, a mechanical framework, a power supply module, a main control module, an in-out bin card box fixing module, a cracking cavity stirring module, a pump module, a magnet module, a valve driving module, a temperature control module and a fluorescent signal acquisition module;
mechanical skeleton the power module is fixed to be set up in the instrument shell, host system the storehouse card box fixed module of cominging in and going out the schizolysis chamber stirring module the pump module the magnet module the valve drive module the temperature control module and fluorescence signal acquisition module is fixed to be set up mechanical skeleton is last, host system with go in and go out storehouse card box fixed module the schizolysis chamber stirring module the pump module the magnet module the valve drive module the temperature control module and fluorescence signal acquisition module the power module is connected.
2. The automatic detection instrument according to claim 1, wherein a display screen, a switch and a data interface are disposed on the instrument housing, the display screen, the switch and the data interface are fixedly disposed on the instrument housing, and the switch is electrically connected to the power module.
3. The fully automatic detection instrument according to claim 2, wherein the data interface is a USB interface.
4. The fully automatic detection instrument according to claim 1, wherein the lysis chamber stirring module is located above the lysis chamber of the microfluidic cartridge, and the lysis chamber stirring module comprises: the heating cavity and the stirring rotor are connected with the guide rail through a motor and used for controlling the heating cavity to move up and down.
5. The fully automatic detection instrument of claim 1, wherein the pump module comprises: the device comprises a first stepping motor, a push rod in transmission connection with the first stepping motor, and a first guide rail.
6. The fully automatic detection instrument of claim 1, wherein the magnet module comprises: the second stepping motor, the magnet cavity, the magnet fixedly arranged in the magnet cavity and the second guide rail.
7. The fully automatic detection instrument of claim 1, wherein the temperature control module comprises: the device comprises a plurality of constant temperature heating tanks, a horizontally fixed third stepping motor, a vertically fixed fourth stepping motor, a third guide rail and a fourth guide rail, wherein the third stepping motor and the third guide rail are used for controlling the front and back movement of the constant temperature heating tanks, and the fourth stepping motor and the fourth guide rail are used for controlling the up and down movement of the constant temperature heating tanks.
8. The fully automatic detection instrument of claim 7, wherein the plurality of constant temperature heating baths comprises: first constant temperature heating bath, second constant temperature heating bath and third constant temperature heating bath, first constant temperature heating bath second constant temperature heating bath and third constant temperature heating bath sets up side by side.
9. The fully automatic detection instrument according to claim 1, wherein the fluorescence signal acquisition module comprises: the device comprises 6 groups of signal acquisition units and a fifth stepping motor, wherein the fifth stepping motor is in transmission connection with the 6 groups of signal acquisition units.
10. The automatic detecting instrument according to claim 9, wherein the 6 groups of signal collecting units are sequentially provided with an LED, a first optical filter, a half-mirror, a second optical filter, and a photodiode.
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| CN202010402603.XA CN111534427A (en) | 2020-05-13 | 2020-05-13 | Full-automatic detection instrument |
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| CN202010402603.XA CN111534427A (en) | 2020-05-13 | 2020-05-13 | Full-automatic detection instrument |
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| CN111534427A true CN111534427A (en) | 2020-08-14 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113652350A (en) * | 2021-08-30 | 2021-11-16 | 成都微康生物科技有限公司 | Full-automatic PCR analysis system for molecular diagnosis by using microfluidic chip |
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| CN103881896A (en) * | 2013-11-01 | 2014-06-25 | 艾康生物技术(杭州)有限公司 | Nucleic acid extractor |
| US20150037803A1 (en) * | 2012-02-10 | 2015-02-05 | Bioneer Corporation | Apparatus and method for automatically analyzing biological samples |
| US20190160468A1 (en) * | 2016-09-12 | 2019-05-30 | Delta Electronics Int'l (Singapore) Pte Ltd | Nucleic acid analysis apparatus |
| CN110184167A (en) * | 2019-07-11 | 2019-08-30 | 北京普创民检生物科技有限公司 | Nucleic acid amplification detection device and system |
| KR20200032639A (en) * | 2018-09-17 | 2020-03-26 | 주식회사 유진셀 | Automated nucleic acid extraction system |
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2020
- 2020-05-13 CN CN202010402603.XA patent/CN111534427A/en active Pending
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| US20150037803A1 (en) * | 2012-02-10 | 2015-02-05 | Bioneer Corporation | Apparatus and method for automatically analyzing biological samples |
| CN103881896A (en) * | 2013-11-01 | 2014-06-25 | 艾康生物技术(杭州)有限公司 | Nucleic acid extractor |
| US20190160468A1 (en) * | 2016-09-12 | 2019-05-30 | Delta Electronics Int'l (Singapore) Pte Ltd | Nucleic acid analysis apparatus |
| KR20200032639A (en) * | 2018-09-17 | 2020-03-26 | 주식회사 유진셀 | Automated nucleic acid extraction system |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113652350A (en) * | 2021-08-30 | 2021-11-16 | 成都微康生物科技有限公司 | Full-automatic PCR analysis system for molecular diagnosis by using microfluidic chip |
| CN113652350B (en) * | 2021-08-30 | 2024-04-26 | 成都微康生物科技有限公司 | Full-automatic PCR analysis system for molecular diagnosis by using microfluidic chip |
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