CN111466387B - Nutrient solution for improving fructification rate, and preparation method and application thereof - Google Patents
Nutrient solution for improving fructification rate, and preparation method and application thereof Download PDFInfo
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- CN111466387B CN111466387B CN202010492978.XA CN202010492978A CN111466387B CN 111466387 B CN111466387 B CN 111466387B CN 202010492978 A CN202010492978 A CN 202010492978A CN 111466387 B CN111466387 B CN 111466387B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/12—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group, wherein Cn means a carbon skeleton not containing a ring; Thio analogues thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/36—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< directly attached to at least one heterocyclic ring; Thio analogues thereof
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Abstract
The invention relates to the field of agriculture, in particular to a nutrient solution for improving maturing rate, a preparation method and application thereof. The nutrient solution for improving the fructification rate comprises the following components in parts by weight: 0.1-2 parts of brassinolide; 0.1-2 parts of diethyl aminoethyl hexanoate; 0.1-3 parts of indole-3-acetic acid; 0.1-2 parts of naphthylacetic acid; 0.1-2 parts of forchlorfenuron; 10-50 parts of C2-C5 alcohol; 50000-120000 parts of water. The nutrient solution is applied to the pollination period of distant hybridization crops of Malvaceae, so that the maturing rate of distant hybridization young bolls is effectively improved, and the shedding rate of the distant hybridization young bolls is reduced.
Description
Technical Field
The invention relates to the field of agriculture, in particular to a nutrient solution for improving maturing rate, a preparation method and application thereof.
Background
Upland cotton is a commercial crop with abundant genetic and phenotypic traits, malvaceae, cotton. Through artificial domestication and directional culture for many years, the genetic basis of upland cotton becomes narrow day by day, and the difficulty of improving the quality and the yield is more and more increased. Distant hybridization is one of the effective ways to achieve the above-mentioned problems.
Abelmoschus manihot, also called Hibiscus manihot and Abelmoschus manihot, is an annual herbaceous plant belonging to the genus Abelmoschus and the family Malvaceae, and has the same unlimited flowering and fruiting habit as cotton. It has natural resistance to aphids and red spiders, can secrete a salt crystal-like particulate matter on the leaf stalk and leaf back, but has no resistance to cotton bollworms. Abelmoschus manihot and upland cotton belong to the same genus of Malvaceae, have close relationship and have similar floral organs and structures. The fruits of Abelmoschus manihot are premature compared to upland cotton. Therefore, the method for introducing the aphid and red spider resistance genes of the abelmoschus manihot into the upland cotton by utilizing the distant hybridization technology is an effective way for breeding new varieties of early-maturing upland cotton with aphid and red spider resistance. However, the distant hybridization has technical and genetic obstacles, so that the seed set rate of the distant hybridization is extremely low, and a positive F1 hybrid is difficult to obtain.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: the nutrient solution is used for improving the maturing rate of distant hybridization young bolls of malvaceae crops and reducing the shedding rate of the distant hybridization young bolls.
The invention provides a nutrient solution for improving fructification rate, which comprises the following components in parts by weight:
0.1-2 parts of brassinolide;
0.1-2 parts of diethyl aminoethyl hexanoate;
0.1-3 parts of indole-3-acetic acid;
0.1-2 parts of naphthylacetic acid;
0.1-2 parts of forchlorfenuron;
10-50 parts of C2-C5 alcohol;
50000-120000 parts of water.
Preferably, the alcohol is ethanol.
Preferably, the method comprises the following steps:
0.5-1.5 parts of brassinolide;
0.5-1.5 parts of diethyl aminoethyl hexanoate;
0.5-2.5 parts of indole-3-acetic acid;
0.5-1.5 parts of naphthylacetic acid;
0.5-1.5 parts of forchlorfenuron;
10-30 parts of ethanol;
80000-100000 parts of water.
Preferably, the method comprises the following steps:
0.7-1.2 parts of brassinolide;
0.5-1.2 parts of diethyl aminoethyl hexanoate;
0.8-2 parts of indole-3-acetic acid;
0.8-1.3 parts of naphthylacetic acid;
0.8-1.4 parts of forchlorfenuron;
10-20 parts of ethanol;
85000-95000 parts of water.
The invention provides a preparation method of nutrient solution for improving fructification rate, which comprises the following steps:
uniformly mixing 0.1-2 parts of brassinolide, 0.1-2 parts of diethyl aminoethyl hexanoate, 0.1-3 parts of indole-3-acetic acid, 0.1-2 parts of naphthylacetic acid, 0.1-2 parts of forchlorfenuron, 10-50 parts of C2-C5 alcohol and 50000-120000 parts of water to obtain the nutrient solution for improving the setting rate.
Preferably, the method specifically comprises the following steps:
adding 0.1-3 parts of indole-3-acetic acid, 0.1-2 parts of naphthylacetic acid and 0.1-2 parts of forchlorfenuron into 10-50 parts of C2-C5 alcohol, and adding 0.1-2 parts of brassinolide, 0.1-2 parts of diethyl aminoethyl hexanoate and 50000-120000 parts of water after complete dissolution.
The invention also provides application of the nutrient solution in the technical scheme in distant hybridization of malvaceae crops.
Preferably, the nutrient solution is applied to the hybridized crop.
Compared with the prior art, the nutrient solution for improving the fructification rate comprises the following components in parts by weight: 0.1-2 parts of brassinolide; 0.1-2 parts of diethyl aminoethyl hexanoate; 0.1-3 parts of indole-3-acetic acid; 0.1-2 parts of naphthylacetic acid; 0.1-2 parts of forchlorfenuron; 10-50 parts of C2-C5 alcohol; 50000-120000 parts of water. The nutrient solution is applied to the pollination period of distant hybridization crops of Malvaceae, so that the maturing rate of distant hybridization young bolls is effectively improved, and the shedding rate of the distant hybridization young bolls is reduced.
Drawings
FIG. 1 shows a photograph of a hybrid bell developed after the grafted seedlings of upland cotton and Abelmoschus manihot are hybridized and injected with a nutrient solution;
FIG. 2 is a photograph showing a cross-bell normally developed but aborted after hybridization of upland cotton and Abelmoschus manihot grafted seedlings;
FIG. 3 is a photograph showing the ringing of a naturally growing Abelmoschus manihot that has developed after self-pollination;
FIG. 4 shows photographs of hybrid plants obtained after the development of hybrid seeds of the invention.
Detailed Description
For a further understanding of the invention, embodiments of the invention are described below in conjunction with the examples, but it should be understood that these descriptions are included merely to further illustrate features and advantages of the invention, and are not intended to limit the invention.
The embodiment of the invention discloses a nutrient solution for improving fructification rate, which comprises the following components in parts by weight:
0.1-2 parts of brassinolide;
0.1-2 parts of diethyl aminoethyl hexanoate;
0.1-3 parts of indole-3-acetic acid;
0.1-2 parts of naphthylacetic acid;
0.1-2 parts of forchlorfenuron;
10-50 parts of C2-C5 alcohol;
50000-120000 parts of water.
The nutrient solution is used in the pollination period of malvaceae distant hybridization. The nutrient solution relates to a plurality of growth promoting hormone active growth regulators, and a plurality of components have synergistic effect so as to improve the young boll setting rate of distant hybridization and reduce the young boll shedding rate.
Wherein, the brassinolide is a high-efficiency plant hormone, and can remarkably increase the growth of the vegetative mass of the plant and promote the fertilization. The nutrient solution comprises 0.1-2 parts of brassinolide; preferably 0.5-1.5 parts of brassinolide; more preferably, the brassinolide content of the brassinolide injection composition is 0.7-1.2 parts.
Diethyl aminoethyl hexanoate can be used to promote cell division and elongation. The nutrient solution comprises 0.1-2 parts of diethyl aminoethyl hexanoate, preferably 0.5-1.5 parts of diethyl aminoethyl hexanoate; more preferably, the diethyl aminoethyl hexanoate is contained in an amount of 0.5-1.2 parts.
Indole-3-acetic acid has positive effects on pollen germination, promotion of pollen tube elongation, and formation of lateral root or flower bud, and can cause parthenocarpy and post-fertilization fruit development. The nutrient solution comprises 0.1-3 parts of indole-3-acetic acid, preferably 0.5-2.5 parts of indole-3-acetic acid; more preferably, the indole-3-acetic acid comprises 0.8-2 parts.
Naphthylacetic acid is a broad-spectrum plant growth regulator, and can promote cell division and enlargement, prevent fruit drop, and change the ratio of female flowers and male flowers. The nutrient solution comprises 0.1-2 parts of naphthylacetic acid, preferably 0.5-1.5 parts of naphthylacetic acid; more preferably, 0.8 to 1.3 parts of naphthylacetic acid is included.
Forchlorfenuron has effects of influencing plant bud development, accelerating cell mitosis, promoting cell enlargement and differentiation, and preventing shedding of fruits and flowers. The nutrient solution comprises 0.1-2 parts of forchlorfenuron, preferably 0.5-1.5 parts of forchlorfenuron; more preferably, 0.8-1.4 parts of forchlorfenuron is included.
According to the invention, the components are dissolved by using C2-C5 alcohol and water as solvents. The C2-C5 alcohol is preferably ethanol.
According to the present invention, preferably, the nutrient solution comprises:
0.5-1.5 parts of brassinolide;
0.5-1.5 parts of diethyl aminoethyl hexanoate;
0.5-2.5 parts of indole-3-acetic acid;
0.5-1.5 parts of naphthylacetic acid;
0.5-1.5 parts of forchlorfenuron;
10-30 parts of ethanol;
80000-100000 parts of water.
According to the present invention, preferably, the nutrient solution comprises:
0.7-1.2 parts of brassinolide;
0.5-1.2 parts of diethyl aminoethyl hexanoate;
0.8-2 parts of indole-3-acetic acid;
0.8-1.3 parts of naphthylacetic acid;
0.8-1.4 parts of forchlorfenuron;
10-20 parts of ethanol;
85000-95000 parts of water.
Embodiments of the invention also include a method of preparing a nutrient solution for enhancing seed set yield, comprising the steps of:
uniformly mixing 0.1-2 parts of brassinolide, 0.1-2 parts of diethyl aminoethyl hexanoate, 0.1-3 parts of indole-3-acetic acid, 0.1-2 parts of naphthylacetic acid, 0.1-2 parts of forchlorfenuron, 10-50 parts of C2-C5 alcohol and 50000-120000 parts of water to obtain the nutrient solution for improving the setting rate.
According to the test and considering the solubility of indole-3-acetic acid, naphthylacetic acid and forchlorfenuron, preferably, 0.1-3 parts of indole-3-acetic acid, 0.1-2 parts of naphthylacetic acid and 0.1-2 parts of forchlorfenuron are firstly added into 10-50 parts of C2-C5 alcohol, and after complete dissolution, 0.1-2 parts of brassinolide, 0.1-2 parts of diethyl aminoethyl hexanoate and 50000-120000 parts of water are added.
The nutrient solution obtained by the method has good uniformity.
The embodiment of the invention also discloses the application of the nutrient solution in the technical scheme in distant hybridization of malvaceae crops. The preferred usage is: the nutrient solution is applied to the hybridized crops.
For further understanding of the present invention, the nutrient solution for increasing the maturing rate, the preparation method thereof and the application thereof provided by the present invention are described in detail with reference to the following examples, and the scope of the present invention is not limited by the following examples.
Example 1
Adding 0.1 part of indole-3-acetic acid, 0.1 part of naphthylacetic acid and 0.1 part of forchlorfenuron into 10 parts of ethanol, and adding 0.1 part of brassinolide, 0.1 part of diethyl aminoethyl hexanoate and 50000 parts of water after complete dissolution to obtain the nutrient solution.
Example 2
Adding 3 parts of indole-3-acetic acid, 2 parts of naphthylacetic acid and 2 parts of forchlorfenuron into 50 parts of ethanol, and adding 2 parts of brassinolide, 2 parts of diethyl aminoethyl hexanoate and 120000 parts of water after complete dissolution to obtain the nutrient solution.
Example 3
Adding 0.7 part of indole-3-acetic acid, 1 part of naphthylacetic acid and 0.8 part of forchlorfenuron into 10 parts of ethanol, and adding 0.5 part of brassinolide, 0.5 part of diethyl aminoethyl hexanoate and 80000 parts of water after complete dissolution to obtain the nutrient solution.
Example 4
Adding 2.5 parts of indole-3-acetic acid, 1.5 parts of naphthylacetic acid and 1.5 parts of forchlorfenuron into 15 parts of ethanol, and adding 1 part of brassinolide, 1.5 parts of diethyl aminoethyl hexanoate and 90000 parts of water after complete dissolution to obtain the nutrient solution.
Example 5
Adding 1.5 parts of indole-3-acetic acid, 1.2 parts of naphthylacetic acid and 1 part of forchlorfenuron into 20 parts of ethanol, and adding 1 part of brassinolide, 1 part of diethyl aminoethyl hexanoate and 100000 parts of water after complete dissolution to obtain the nutrient solution.
Example 6
And (3) taking down the flowers after the upland cotton pollen is scattered the next day after emasculation, tearing off the corolla and the calyx, uniformly coating the pollen on the stigma of the flower bud of the emasculated abelmoschus manihot grafted seedling, and applying different nutrient solutions to different grafted seedlings, wherein the nutrient solutions are prepared in the embodiments 1-5. The nutrient solution prepared in each example is applied to the grafted seedlings respectively, and the number of emasculation and pollination hybrid bolls is ensured to be more than 200.
Injecting 5 points of the nutrient solution prepared in the embodiment into the base part of the pistil by using a micro injector, wherein each point is 5-8 ul, cutting the cotton crowns of the upland cotton into a square block with the size of 2cm by using scissors, uniformly coating the pollen of the upland cotton on the square block, finally wrapping the stigma of the grafted seedling by using the square block, and bundling the stigma, the coating square block and the calyx by using a red rope, so that the isolated pollen is prevented from being directly exposed in solar ultraviolet rays and losing vigor prematurely.
The growth and development of the hybrid bolls, including the normal development and the number of shed hybrid bolls, are recorded daily.
After about 35 days, before the grafted seedling hybrid bells are changed from green to black and are cracked, each hybrid bell and the hanging tag are independently put into a net sand bag, the net sand bag is placed under sunlight for airing until the hybrid bells are naturally cracked, the shell is removed, and the hybrid bell seeds are collected. The number of seeds recorded is the average number of seeds obtained from the hybrid.
Although the hybrid boll obtained by the method (shown in figure 1) does not reach the hybrid seed obtained by self-pollination of the abelmoschus manihot (shown in figure 3), the hybrid seed obtained by the method is successful and can meet the requirement of the test.
After the upland cotton and the abelmoschus manihot are subjected to distant hybridization, the nutrient solution is not injected, and finally the obtained hybrid boll is shown in figure 2 and basically has no developed and formed seeds.
The method is characterized in that nutrient solution is not applied after the upland cotton and the abelmoschus manihot are subjected to distant hybridization, the nutrient solution is applied after the upland cotton and the abelmoschus manihot are subjected to distant hybridization, the conditions of hybrid seeds obtained in the three conditions of self-pollination of the abelmoschus manihot are counted, and the statistical results are shown in table 1.
TABLE 1 comparison of ring-up rates for different treatment methods
And (3) performing single-seed sowing on the hybrid seeds obtained in the examples 1-5, placing the hybrid seeds in an illumination incubator, alternating illumination and dark environment for 12 hours respectively at the temperature of 25-35 ℃, and recording the emergence date and the germination rate of the hybrid seeds. After the hybrid seeds germinate, the hybrid seeds Abelmoschus manihot seeds and upland cotton seeds are simultaneously sown in the field in a single-seed mode, and the emergence date and the emergence rate of each species are recorded. And observing and recording the hybrid seedlings every day, wherein the hybrid seedlings have the surface characteristics of Abelmoschus manihot and upland cotton. After the hybrid seeds, the abelmoschus manihot and the upland cotton grow true leaves, extracting DNA of the leaves, screening primers, and detecting the hybrid seeds to be positive.
The hybrid plants have the parental trait, see in particular fig. 4. As can be seen from FIG. 4, the hybrid seeds obtained by the present invention have fertility and can be used for screening to obtain new homozygous plant resources. Comparative example 1
1.5 parts of naphthylacetic acid and 1.5 parts of forchlorfenuron are added into 15 parts of ethanol, and after complete dissolution, 1 part of brassinolide, 1.5 parts of diethyl aminoethyl hexanoate and 90000 parts of water are added to obtain a mixed solution 1.
Comparative example 2
Adding 2.5 parts of indole-3-acetic acid and 1.5 parts of forchlorfenuron into 15 parts of ethanol, completely dissolving, and then adding 1 part of brassinolide, 1.5 parts of diethyl aminoethyl hexanoate and 90000 parts of water to obtain a mixed solution 2.
Comparative example 3
Adding 2.5 parts of indole-3-acetic acid, 1.5 parts of naphthylacetic acid and 1.5 parts of forchlorfenuron into 15 parts of ethanol, and adding 1.5 parts of diethyl aminoethyl hexanoate and 90000 parts of water after complete dissolution to obtain a mixed solution 3.
Comparative example 4
2.5 parts of indole-3-acetic acid and 1.5 parts of naphthylacetic acid are added into 15 parts of ethanol, and after complete dissolution, 1.5 parts of diethyl aminoethyl hexanoate and 90000 parts of water are added to obtain a mixed solution 4.
And (3) taking down flowers after the upland cotton pollen is scattered the next day after emasculation, tearing off corolla and calyx, uniformly coating the pollen on the stigma of the flower bud of the emasculated abelmoschus manihot grafted seedling, and applying different nutrient solutions to different grafted seedlings, wherein the mixed solution is prepared according to the comparative examples 1-4. The mixed solution prepared in each example is applied to grafted seedlings respectively, and the number of emasculation and pollination hybrid bolls is ensured to be more than 200.
Injecting 5 points of the mixed liquid prepared in the comparative examples 1-4 into the base part of the pistil by a micro injector, wherein each point is 5-8 ul, cutting the cotton crowns of the upland cotton into a square block with the size of 2cm by using scissors, uniformly coating the pollen of the upland cotton on the square block, finally wrapping the stigma of the grafted seedling by using the square block, and bundling the stigma, the coating square block and the calyx by using a red rope, so as to avoid that the isolated pollen directly exposes in solar ultraviolet rays and loses activity prematurely.
The growth and development of the hybrid bolls, including the normal development and the number of shed hybrid bolls, are recorded daily.
After about 35 days, before the grafted seedling hybrid bells are changed from green to black and are cracked, each hybrid bell and the hanging tag are independently put into a net sand bag, the net sand bag is placed under sunlight for airing until the hybrid bells are naturally cracked, the shell is removed, and the hybrid bell seeds are collected.
And (3) counting the conditions of the hybrid seeds obtained after the mixed liquids prepared in the comparative examples 1-4 are applied respectively, and the statistical results are shown in table 2.
TABLE 2 comparison of Bell formation rates of comparative examples 1 to 4 in different treatment methods
| Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | |
| Pollination bell number | 210 | 210 | 216 | 205 |
| Developmental bell number (number) | 14 | 12 | 11 | 13 |
| Percentage of finished bell (%) | 6.7 | 5.7 | 5.1 | 6.3 |
| Hybrid seed (granule) | 40 | 42 | 38 | 46 |
| Single bell for getting seed number | 2.86 | 3.5 | 3.45 | 3.5 |
According to experimental results of examples and comparative examples, the nutrient solution prepared by the invention can realize synergistic effect among components, effectively improve the seed setting rate of young bolls obtained by distant hybridization of upland cotton and abelmoschus manihot, and reduce the shedding rate of young bolls obtained by distant hybridization.
The above description of the embodiments is only intended to facilitate the understanding of the method of the invention and its core idea. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (8)
1. The nutrient solution for improving the fructification rate is characterized by comprising the following components in parts by weight:
0.1-2 parts of brassinolide;
0.1-2 parts of diethyl aminoethyl hexanoate;
0.1-3 parts of indole-3-acetic acid;
0.1-2 parts of naphthylacetic acid;
0.1-2 parts of forchlorfenuron;
10-50 parts of C2-C5 alcohol;
50000-120000 parts of water.
2. The nutritional solution according to claim 1, wherein the C2-C5 alcohol is ethanol.
3. The nutrient solution as claimed in claim 2, comprising:
0.5-1.5 parts of brassinolide;
0.5-1.5 parts of diethyl aminoethyl hexanoate;
0.5-2.5 parts of indole-3-acetic acid;
0.5-1.5 parts of naphthylacetic acid;
0.5-1.5 parts of forchlorfenuron;
10-30 parts of ethanol;
80000-100000 parts of water.
4. The nutrient solution as claimed in claim 2, comprising:
0.7-1.2 parts of brassinolide;
0.5-1.2 parts of diethyl aminoethyl hexanoate;
0.8-2 parts of indole-3-acetic acid;
0.8-1.3 parts of naphthylacetic acid;
0.8-1.4 parts of forchlorfenuron;
10-20 parts of ethanol;
85000-95000 parts of water.
5. A preparation method of nutrient solution for improving fructification rate is characterized by comprising the following steps:
uniformly mixing 0.1-2 parts of brassinolide, 0.1-2 parts of diethyl aminoethyl hexanoate, 0.1-3 parts of indole-3-acetic acid, 0.1-2 parts of naphthylacetic acid, 0.1-2 parts of forchlorfenuron, 10-50 parts of C2-C5 alcohol and 50000-120000 parts of water to obtain the nutrient solution for improving the setting rate.
6. The method according to claim 5, characterized in that it comprises in particular the steps of:
adding 0.1-3 parts of indole-3-acetic acid, 0.1-2 parts of naphthylacetic acid and 0.1-2 parts of forchlorfenuron into 10-50 parts of C2-C5 alcohol, and adding 0.1-2 parts of brassinolide, 0.1-2 parts of diethyl aminoethyl hexanoate and 50000-120000 parts of water after complete dissolution.
7. Use of the nutrient solution according to any one of claims 1 to 4 for distant hybridization of malvaceae crops.
8. Use according to claim 7, wherein the nutrient solution is applied to the hybridized crop.
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