CN111443195A

CN111443195A - Immunoassay reagent card

Info

Publication number: CN111443195A
Application number: CN202010254558.8A
Authority: CN
Inventors: 严义勇; 马红圳; 蔡振庆; 陈奕耀; 金虹
Original assignee: SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD
Current assignee: SHENZHEN BIOEASY BIOTECHNOLOGY CO LTD
Priority date: 2020-04-02
Filing date: 2020-04-02
Publication date: 2020-07-24

Abstract

The application provides an immunoassay reagent card, including casing, test paper strip, sealing plug and kit structure, the test paper strip has been seted up sample addition port and visual window in being fixed in the airtight cavity of casing on the casing, and the kit structure includes that one end is fixed in sleeve and the reagent cup of slidable mounting on the casing in the sleeve, is equipped with the puncture piece that is used for puncturing the blind end of reagent cup in the cavity. The application provides an immunoassay reagent card, when carrying out immunodetection, the sample utilizes the sealing plug to seal the sample addition port on releasing the test paper strip through the sample addition port, avoids taking place the aerosol exchange, when improving the accuracy of testing and analyzing, can effectively stop to cause the infection to the operator, and can not cause the pollution to the environment. When the reagent is released to the test strip, the reagent cup in the sleeve is pressed towards the cavity of the shell by using external force, the closed end of the reagent cup can be punctured by the puncturing piece, and the reagent in the reagent cup can be quickly released to the test strip.

Description

Immunoassay reagent card

Technical Field

The application belongs to the technical field of immunochromatography, and particularly relates to an immunoassay reagent card.

Background

The immunochromatography technology has the advantages of high detection speed, simple operation, low cost and the like, and becomes a common technical means in detection of food safety, medical treatment and the like. When the current immunoassay reagent card is used for carrying out biochemical immunoassay on a sample, a liquid transferring instrument such as a liquid transferring gun is generally adopted to respectively transfer and release the sample and the reagent to a sample adding port, and the sample and the reagent in the sample adding port are uniformly mixed and then carry out chromatography reaction through a chromatography paper strip.

However, since the open sample addition port is directly communicated with the external environment, particularly in the process of carrying out high-sensitivity biochemical immunoassay, not only is aerosol exchange easily generated, but also interference of external pollutants on detection and analysis is generated, so that the detection result is inaccurate; moreover, volatile harmful substances in the reagent (such as biochemical reagents which are liable to cause biochemical damage) can cause pollution to the external environment, and if the sample contains substances (such as highly infectious germs, viruses and the like) which can be transmitted by aerosol, the reagent card in the waiting chromatography process and after use risks infecting operators in the transferring process.

Disclosure of Invention

An object of the embodiment of the application is to provide an immunoassay reagent card to solve the problem that in the process of carrying out biochemical immunoassay, the immunoassay reagent card existing in the prior art is not only easy to generate aerosol exchange and causes inaccurate detection results, but also has the risk of infecting operators and polluting the environment.

In order to achieve the purpose, the technical scheme adopted by the application is as follows: provided is an immunoassay reagent card comprising:

the device comprises a shell, a sample inlet and a sample outlet, wherein the shell is provided with a closed cavity and the sample inlet is communicated with the cavity;

the test strip is placed and fixed in the cavity;

the sealing plug is used for sealing the sample adding port; and

the reagent kit structure is used for sealing and packaging reagents in advance and comprises a sleeve with one end fixed on the shell and a reagent cup slidably arranged in the sleeve;

wherein, the shell is provided with a visual window for observing the test condition of the test strip, the shell is also provided with an opening for the closed end of the reagent cup to pass through, the opening is communicated with the cavity, and the cavity is correspondingly provided with a puncture piece for puncturing the closed end of the reagent cup.

Optionally, a storage pool for accommodating a reagent is arranged in the cavity corresponding to the opening, the opening is communicated with the storage pool, and one end of the test strip is arranged in the storage pool.

Optionally, a bayonet for clamping the test strip is formed in the side wall of the storage pool, and one end of the test strip passes through the bayonet and extends into the storage pool.

Optionally, the bottom of the storage pool is provided with a positioning groove for accommodating and positioning the test strip.

Optionally, the piercing member is a post having one end fixed in the cavity and the other end extending into the opening.

Optionally, the diameter of the column decreases from the end remote from the reagent cup to the end close to the reagent cup.

Optionally, the reagent kit structure further comprises a sliding structure for guiding the reagent cup to move in the sleeve, the sliding structure comprises a sliding block and a sliding groove in sliding fit with the sliding block, the sliding block is convexly arranged on the outer wall of the reagent cup, and the sliding groove is concavely arranged on the inner wall of the sleeve.

Optionally, the casing include the inferior valve and with the inferior valve can be dismantled the upper cover that links to each other, the sample addition mouth the visual window with the opening is located respectively on the upper cover, telescopic one end encircles the opening sets up and is fixed in on the upper cover, the upper cover with inferior valve sealing connection forms inclosed the cavity.

Optionally, the reagent kit structure further comprises a piston structure for pushing the reagent cup towards the cavity, the piston structure is slidably mounted in the sleeve, one end of the reagent cup facing away from the closed end is an open end, and an end of the piston structure is plugged on the open end of the reagent cup.

Optionally, the piston structure includes a piston body and a push rod connected to the piston body, an end portion of the piston body, which is far away from one end of the push rod, is blocked at an open end of the reagent cup, and an end of the push rod, which is far away from the piston body, protrudes out of a port at the other end of the sleeve.

Compared with the prior art, one or more technical solutions in the embodiments of the present application have at least one of the following technical effects:

this application immunodetection reagent card, at the in-process that carries out biochemical immunodetection, release the sample on the test paper strip in the airtight cavity of casing through the sample addition mouth, and utilize the sealing plug to seal the sample addition mouth, avoid the sample addition mouth directly to communicate with external environment and take place the aerosol exchange, and then avoid external pollutant to the interference of detection and analysis, improve the accuracy of detection and analysis, prevent the high infectious germ that contains in the sample simultaneously, virus etc. passes through the aerosol and spreads, can effectively stop to cause the condition emergence of infection to the operator, and the fail safe nature is higher. When the reagent is released to the test strip in the closed cavity of the shell, the reagent cup in the sleeve is pressed towards the cavity of the shell, the closed end of the reagent cup can be punctured by the puncturing piece in the cavity, and the reagent packaged in the reagent cup can be quickly released to the test strip. In addition, the structure of the kit is a closed space, so that the pollution of volatile harmful substances in the reagent to the environment can be avoided.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the embodiments or the prior art descriptions will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise.

FIG. 1 is a schematic perspective view of an immunoassay reagent card provided in an embodiment of the present application;

FIG. 2 is a schematic top view of an immunoassay reagent card according to an embodiment of the present disclosure;

FIG. 3 is a schematic cross-sectional view taken along line A-A of FIG. 2;

FIG. 4 is an enlarged, fragmentary, schematic view of FIG. 3;

FIG. 5 is an exploded view of an immunoassay reagent card provided in an embodiment of the present application;

FIG. 6 is an enlarged partial schematic view of FIG. 5;

FIG. 7 is a schematic perspective view of an assembly sleeve of an upper cover according to an embodiment of the present disclosure;

FIG. 8 is an enlarged, fragmentary, schematic view of FIG. 7;

FIG. 9 is a schematic perspective view of a reagent cup provided in an embodiment of the present application;

fig. 10 is a schematic perspective view of a piston structure according to an embodiment of the present application.

Wherein, in the figures, the respective reference numerals:

1-test paper strip;

2-a shell; 21-a lower shell; 211-a plunger; 22-upper cover; 221-a sample addition port; 222-a viewable window; 223-opening; 224-a jack; 23-a cavity;

3-sealing the plug;

4-kit structure; 41-a sleeve; 411-a chute; 42-reagent cup; 421-open end; 422-closed end; 423-a slide block; 43-piston configuration; 431-a piston body; 432-a push rod; 433-ring groove;

5-a piercing member;

6-sealing ring;

7-a clamping structure; 71-a splint; 72-clamping;

8-a pressing column;

9-a storage pool; 91-bayonet; 92-positioning groove.

Detailed Description

In order to make the technical problems, technical solutions and advantageous effects to be solved by the present application clearer, the present application is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present application and are not intended to limit the present application.

It will be understood that when an element is referred to as being "connected" or "disposed" to another element, it can be directly on the other element or be indirectly connected to the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.

In the description of the present application, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.

It will be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like, as used herein, refer to an orientation or positional relationship indicated in the drawings that is solely for the purpose of facilitating the description and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be considered as limiting the present application.

Referring to fig. 1 to 3 and 4 together, the immunoassay reagent card provided in the embodiment of the present application will now be described. The immunodetection reagent card provided by the embodiment of the application can be suitable for testing the dangers such as pathogenic bacteria with strong infectivity, viruses and chemical reagents with strong volatility, and can avoid causing harm to human bodies in the processes of reagent card transportation and waste treatment. The immunoassay reagent card provided by the embodiment of the application comprises a test strip 1, a shell 2, a sealing plug 3 and a kit structure 4, wherein a closed cavity 23 is arranged inside the shell 2, and the test strip 1 is placed and fixed in the cavity 23. Referring to fig. 4, the casing 2 is provided with a sample port 221 communicated with the cavity 23, and the sealing plug 3 is used for sealing the sample port 221 so as to avoid aerosol exchange during the process of performing biochemical immunoassay. Referring to fig. 2, the casing 2 is provided with a closed visible window 222, so that a user can observe the test condition of the test strip 1 through the visible window 222. The kit structure 4 is used for sealing and encapsulating the reagent in advance, reducing the contact between the internal system of the immunoassay reagent card and the external environment, and preventing the pollution of volatile harmful substances in the reagent to the external environment. With further reference to fig. 4, the reagent cartridge arrangement 4 comprises a sleeve 41 having one end fixed to the housing 2 and a reagent cup 42 slidably mounted in the sleeve 41, the housing 2 further defines an opening 223 for the closed end 422 of the reagent cup 42 to pass through, the opening 223 being in communication with the cavity 23, so that the piston arrangement 43 pushes the closed end 422 of the reagent cup 42 and places it in the cavity 23. With further reference to fig. 4 and 6, a piercing member 5 for piercing the closed end 422 of the reagent cup 42 is correspondingly provided in the cavity 23. In use, the reagent cup 42 is pushed towards the cavity 23 of the housing 2 using an external force, causing the closed end 422 of the reagent cup 42 to move and be placed in the cavity 23, the closed end 422 of the reagent cup 42 being pierced by the piercing member 5 in the cavity 23, causing the reagent sealingly enclosed in the reagent cup 42 to flow into the cavity 23.

The application provides an immunoassay reagent card, compared with the prior art, at the in-process that carries out biochemical immunoassay, release the sample on test paper strip 1 in the airtight cavity 23 of casing 2 through sample addition port 221, and utilize sealing plug 3 to seal sample addition port 221, avoid sample addition port 221 directly to communicate with the external environment and take place the aerosol exchange, and then avoid external pollutant to the interference of detection and analysis, improve the accuracy of detection and analysis, prevent the high infectious germ that contains in the sample simultaneously, virus etc. passes through the aerosol and spreads, can effectively stop to cause the condition emergence of infection to the operator, fail safe nature is higher. When the reagent is released to the test strip 1 in the closed cavity 23 of the housing 2, the reagent cup 42 in the sleeve 41 is pressed towards the cavity of the housing, the puncture piece 5 in the cavity 23 can puncture the closed end 422 of the reagent cup 42, and the reagent packaged in the reagent cup 42 can be quickly released to the test strip 1, so that the operation is simple and convenient, and the detection efficiency is improved. Moreover, the reagent box structure 4 is a closed space, which can prevent volatile harmful substances in the reagent from polluting the environment and influencing the health of human body. In addition, after the detection is finished, the sample and the reagent are stored in the closed cavity 23 of the shell 2 in a sealing manner, so that the difficulty in collecting and treating the subsequent detection waste is reduced, and the biological infection hazard to operators generated in the process of collecting, transferring and treating the waste is avoided.

It will be appreciated that in one embodiment the closed end 422 of the reagent cup 42 may be a plastic part which is integrally formed with the reagent cup 42 and which is easily pierced or punctured by the puncturing element 5. In another embodiment, the closed end 422 of the reagent cup 42 may also be a sealing sheet or membrane, such as an aluminium or plastic film, which is closed onto the respective port of the reagent cup 42 and which is easily pierced or punctured by the puncturing element 5. In another embodiment, the closed end 422 of the reagent cup 42 may also be provided with a soft gel member made of a material such as silicone or rubber which is easily pierced or punctured by the puncturing member 5. In addition, considering that the soft plastic member with a large thickness is generally difficult to be punctured by the puncturing member 5 rapidly, so that the reagent in the reagent cup 42 cannot be released into the cavity 23 of the housing 2 rapidly, it is also possible to provide a liquid outlet hole on the end surface of the closed end 422 of the reagent cup 42, and to provide a sealing sheet in the liquid outlet hole, which can be punctured by the puncturing member 5. Correspondingly, the puncturing part 5 can be a column or a rod with a sharp end at one end, or a knife-edge-shaped puncturing strip at one end, and the like, and the specific structure of the puncturing part 5 is not limited uniquely here, and can be selected and arranged according to actual needs.

In another embodiment of the present application, referring to fig. 5, 7 to 8, the housing 2 includes a lower shell 21 and an upper cover 22 detachably connected to the lower shell 21, the sample adding port 221, the viewing window 222 and the opening 223 are respectively disposed on the upper cover 22, one end of the sleeve 41 is disposed around the opening 223 and fixed on the upper cover 22, and the upper cover 22 and the lower shell 21 are hermetically connected to form the closed cavity 23.

In this embodiment, the casing 2 is configured as a lower casing 21 and an upper cover 22 detachably connected to the lower casing 21, wherein the upper cover 22 and the lower casing 21 are combined together in a sealing and bonding manner, so that the upper cover 22 and the lower casing 21 are hermetically connected to form a sealed cavity 23, and the test strip 1 is conveniently mounted and clamped in and fixed to the sealed cavity 23. In addition, the sample addition port 221, the visual window 222 and the opening 223 are respectively arranged on the upper cover 22, so that one end of the sleeve 41 is arranged around the opening 223 and fixed on the upper cover 22, which is beneficial to the processing and manufacturing of the immunity detection reagent card and reduces the production cost.

Specifically, in another embodiment of the present application, referring to fig. 5, the viewing window 222 is formed by hermetically installing a transparent sheet, such as a transparent glass sheet, a transparent film or a transparent film, in the viewing window of the upper cover 22, so that the cavity 23 of the housing 2 forms a closed space environment. The transparent glass sheet, the transparent film or the transparent film may be integrally formed with the upper cover 22 to facilitate manufacturing and improve sealing performance. Of course, the transparent glass sheet, the transparent film or the transparent film may be sealed and bonded to the viewing window of the upper cover 22 by adhesion.

In another embodiment of the application, referring to fig. 1, 4 and 5 together, the reagent cartridge structure 4 further comprises a piston structure 43 for pushing the reagent cup 42 towards the cavity 23, the piston structure 43 being slidably mounted in the sleeve 41, an end of the reagent cup 42 facing away from the closed end 422 being an open end 421, and an end of the piston structure 43 being closed off the open end 421 of the reagent cup 42.

In this embodiment, the reagent kit structure 4 further includes a piston structure 43 for pushing the reagent cup 42 toward the cavity 23, so that when releasing the reagent onto the test strip 1 in the sealed cavity 23 of the housing 2, the piston structure 43 in the sleeve 41 is only pressed to push the reagent cup 42 to move and place the reagent cup into the cavity 23 of the housing 2, the puncturing part 5 in the cavity 23 can puncture the closed end 422 of the reagent cup 42, and the reagent packaged in the reagent cup 42 can be quickly released onto the test strip 1. Moreover, the end of the reagent cup 42 facing away from the closed end 422 is provided with an open end 421, and the end of the piston structure 43 is plugged on the open end 421 of the reagent cup 42, so that the reagent cup 42 forms a closed space, thereby preventing the volatile harmful substances in the reagent from polluting the environment and affecting the health of human body. In addition, after the detection is finished, because the piston structure 43 is in sealing fit with the sleeve 41, the sample and the reagent are stored in the sealed cavity 23 of the shell 2 in a sealing manner, the difficulty in collecting and treating the subsequent detection waste is reduced, and the biological infection harm to an operator in the process of collecting, transferring and treating the waste is avoided.

In another embodiment of the present application, referring to fig. 4 and 10, the piston structure 43 includes a piston body 431 movably installed in the sleeve 41 and a push rod 432 connected to the piston body 431, an end of the piston body 431 is sealed to the open end 421 of the reagent cup 42, and an end of the push rod 432 away from the piston body 431 protrudes from a port at the other end of the sleeve 41.

In this embodiment, the piston structure 43 comprises a piston body 431 and a push rod 432 connected to the piston body 431, and one end of the push rod 432 away from the piston body 431 is protruded out of the port of the other end of the sleeve 41, so that the user can press the push rod 432 to quickly push the reagent cup 42 to move toward the closed cavity 23 of the housing 2, thereby facilitating the column to quickly puncture the closed end 422 of the reagent cup 42 and improving the detection efficiency.

In another embodiment of the present application, to avoid the leakage or volatilization of the reagents in the reagent cup 42, the open end 421 of the reagent cup 42 is sealed and closed by an aluminum foil or plastic film after the reagents are added, and the end of the piston body 431 is pressed against the open end 421 of the reagent cup 42, so as to enhance the sealing performance of the aluminum foil or plastic film on the reagent cup 42 and avoid the pollution of the environment by the volatile harmful substances in the reagents.

In another embodiment of the present application, referring to fig. 4 and 10, a ring groove 433 is formed at a connection portion of the push rod 432 and the piston body 431, and a sealing ring 6 is installed in the ring groove 433.

In this embodiment, the annular groove 433 is disposed at the connection position between the push rod 432 and the piston body 431, and the sealing ring 6 is installed in the annular groove 433, so that the sealing performance between the piston structure 43 and the inner wall of the sleeve 41 is enhanced, the leakage of the reagent during the transportation or detection using process is prevented, and the pollution of volatile harmful substances in the reagent to the environment and the influence on the health of the human body can be further avoided. In addition, after the detection is finished, the sample and the reagent are stored in the closed cavity 23 of the shell 2 in a sealing manner, so that the difficulty in collecting and treating the subsequent detection waste is reduced, and the biological infection hazard to operators generated in the process of collecting, transferring and treating the waste is avoided.

In another embodiment of the present application, referring to fig. 3 and fig. 5, a plurality of insertion rods 211 are disposed on the lower case 21, and insertion holes 224 into which the insertion rods 211 are inserted are correspondingly disposed on the upper cover 22, respectively.

In this embodiment, a plurality of insertion rods 211 are disposed on the housing, and the insertion holes 224 for inserting the insertion rods 211 are correspondingly disposed on the upper cover 22, so that the upper cover 22 can be quickly connected to the lower housing 21 only by inserting the insertion rods 211 into the corresponding insertion holes 224, respectively, with simple operation and easy assembly and disassembly. Of course, the upper cover 22 and the lower cover 21 can also be connected by a fastening structure known to those skilled in the art, and the specific structural form of the fastening structure can be selected and arranged according to actual needs, which is not described herein again.

In another embodiment of the present application, please refer to fig. 5, a position-locking structure 7 for positioning one end of the test strip 1 away from the storage pool 9 is disposed on the inner bottom surface of the lower casing 21.

In this embodiment, through be equipped with clamping structure 7 on the interior bottom surface of inferior valve 21, keep away from reservoir 9 one end with test paper strip 1 and arrange clamping structure 7 in to keep away from reservoir 9 one end to test paper strip 1 and play the positioning action, can improve the stability of imbibition pad of test paper strip 1, be favorable to the higher reagent of viscosity or sample to flow along the length direction unblocked of test paper strip 1, improve chromatography detection effect.

Specifically, in another embodiment of the present application, please refer to fig. 5, the clamping structure 7 includes two parallel clamping plates 71 disposed at an interval, and a clamping portion 72 is formed between the two clamping plates 71 for accommodating and positioning one end of the test strip 1 away from the storage tank 9. Of course, the clamping structure 7 may also be a clamping groove directly formed on the fixing block fixed in the lower case 21, and the end of the test strip 1 far from the storage tank 9 is placed in the clamping groove, so that the end of the test strip 1 far from the storage tank 9 can be positioned. Furthermore, the inner bottom surface of the lower shell 21 is also provided with a reinforcing rib for supporting the test strip 1, so that the stability of the liquid absorption pad of the test strip 1 is enhanced, a reagent or a sample with high viscosity can smoothly flow along the length direction of the test strip 1, and the chromatography detection effect is improved.

In another embodiment of the present application, referring to fig. 3 and fig. 7, a plurality of pressing pillars 8 for pressing the test strip 1 are further disposed on the upper cover 22 at intervals, so that the test strip 1 can be pressed and fixed in the lower casing 21 by the plurality of pressing pillars 8, and since the contact area between each pressing pillar 8 and the test strip 1 is small, the sample reagent or product with high viscosity flows more smoothly along the length direction of the test strip 1, thereby improving the detection efficiency.

In this embodiment, a plurality of pressing columns 8 for pressing against the test strip 1 are further arranged on the upper cover 22 at intervals, the test strip 1 is pressed and fixed in the lower shell 21 through the plurality of pressing columns 8, and because the contact area between each pressing column 8 and the test strip 1 is small, the phenomenon that the reagent and the sample are blocked transversely to generate multi-directional overflow is avoided, so that the reagent and the sample can flow along the length direction of the test strip 1 smoothly.

In another embodiment of the present application, referring to fig. 3 and fig. 7, each of the pressing pillars 8 is a cylinder structure, and the diameter of each of the pressing pillars 8 is smaller than the width of the test strip 1.

In this embodiment, each support the compression column 8 and set up to the cylinder structure, strengthen each support the compression column 8 and support the dynamics that compresses tightly test paper strip 1, make test paper strip 1 clamp tightly between upper cover 22 and inferior valve 21, make test paper strip 1 difficult to remove. Moreover, the diameter of each pressing column 8 is set to be smaller than the width of the test strip 1, so that the contact area between each pressing column 8 and the test strip 1 is reduced, the phenomenon that the reagent and the sample are blocked by longitudinal (parallel to the thickness direction of the test strip 1) extrusion to generate multi-directional flow is avoided, the smooth flow of the reagent and the sample along the length direction of the test strip 1 is facilitated, and the detection efficiency is improved.

In another embodiment of the present application, please refer to fig. 3 and fig. 7, the plurality of pressing pillars 8 are distributed at intervals along the length direction of the test strip 1, the axial direction of each pressing pillar 8 is parallel to the thickness direction of the test strip 1, and one end of each pressing pillar 8 away from the upper cover 22 is pressed against the test strip 1.

In this embodiment, through a plurality of support compression posts 8 along the length direction interval distribution of test paper strip 1, and each support compression post 8 axial is on a parallel with the thickness direction of test paper strip 1, and each support compression post 8 is kept away from the one end of upper cover 22 and is sticiss to support on test paper strip 1, makes test paper strip 1 receive balanced support and compresses tightly the dynamics, when reinforcing test paper strip 1 stability, is favorable to reagent and sample to a certain extent to flow along the length direction smoothness of test paper strip 1, and then is favorable to improving detection efficiency.

In another embodiment of the present application, referring to fig. 3 and fig. 6, a storage pool 9 for containing a reagent is disposed in the cavity 23 at a position corresponding to the opening 223, the opening 223 is communicated with the storage pool 9, and one end of the test strip 1 is disposed in the storage pool 9.

In this embodiment, by providing the reservoir 9 in the cavity 23 and the position of the reservoir 9 corresponding to the position of the opening 223, the reagent released from the reagent cup 42 may be stored in the reservoir 9 after the closed end 422 of the reagent cup 42 has been pierced, thereby avoiding the occurrence of a flooding of the reagent in the cavity 23 of the housing 2. In addition, one end of the test strip 1 is placed in the storage pool 9, and the reagent in the storage pool 9 can be uniformly mixed with the sample in a lateral flow (flowing along the length direction of the test strip 1) mode through the test strip 1, which is beneficial to improving the detection accuracy.

In another embodiment of the present application, please refer to fig. 6, a side wall of the storage pool 9 is provided with a bayonet 91 for holding the test strip 1, and one end of the test strip 1 passes through the bayonet 91 and extends into the storage pool 9.

In this embodiment, the side wall of the storage pool 9 is provided with the bayonet 91, and the bayonet 91 is utilized to clamp and position the part of the test strip 1 placed in the storage pool 9, so that the stability of the test strip 1 is enhanced, smooth flowing of a reagent along the length direction of the test strip 1 is facilitated, and the detection efficiency and the detection effect are improved.

In another embodiment of the present application, referring to fig. 6, the bottom of the storage tank 9 is provided with a positioning groove 92 for accommodating and positioning the test strip 1.

In this embodiment, through being equipped with constant head tank 92 in the bottom of reservoir 9, the one end holding that places test paper strip 1 in reservoir 9 is in constant head tank 92 for test paper strip 1 has great area of contact with the reagent, strengthens test paper strip 1's stability, is favorable to the unblocked flow of reagent along the length direction of test paper strip 1, improves detection efficiency and detection effect.

In another embodiment of the present application, referring to fig. 4, the test strip 1 includes a liquid absorption pad and a bottom plate supporting the liquid absorption pad, and the bottom plate is supported on the inner bottom surface of the lower case 21.

In this embodiment, the liquid absorption pad is supported in the lower case 21 by the bottom plate, so that the stability of the liquid absorption pad can be improved, and a sample with high viscosity can smoothly flow along the length direction of the test strip 1. Through being equipped with constant head tank 92 in the bottom of reservoir 9, with the bottom plate holding in constant head tank 92, can avoid the reagent contact in bottom plate and the reservoir 9, only make the imbibition pad that supports on the bottom plate directly contact with the reagent in the reservoir 9 for test paper strip 1 has great area of contact with the reagent, and then is favorable to the homogeneous mixing of reagent and sample, improves detection efficiency.

In another embodiment of the present application, referring to fig. 4 and 6, the piercing member 5 is a cylinder with one end fixed in the cavity 23 and the other end extending into the opening 223.

In this embodiment, the puncturing part 5 is a cylinder, one end of the cylinder is fixed in the cavity 23, and the other end of the cylinder extends into the opening 223, so that when the piston structure 43 pushes the reagent cup 42 to move towards the cavity 23 to the opening 223, the closed end 422 of the reagent cup 42 can be punctured by the cylinder, the reagent packaged in the reagent cup 42 can be released onto the test strip 1 quickly, the operation is simple and convenient, and the detection efficiency is improved.

In another embodiment of the present application, and referring also to fig. 6, the diameter of the column decreases from the end remote from the reagent cup 42 to the end close to the reagent cup 42.

In this embodiment, the diameter of the column is gradually reduced from the end far away from the reagent cup 42 to the end near the reagent cup 42, so that the end near the reagent cup 42 is pointed, the contact area between the column and the reagent cup 42 is reduced, and the closed end 422 of the reagent cup 42 is rapidly punctured by the column, so that the reagent packaged in the reagent cup 42 is rapidly released onto the test strip 1, the operation is simple and convenient, and the detection efficiency is improved.

In another embodiment of the present application, please refer to fig. 4 and fig. 6 together, the cylinder is located in the storage pool 9, and the cylinder is axially parallel to the axial direction of the sleeve 41, so as to ensure that the cylinder can puncture the closed end 422 of the reagent cup 42, and simultaneously, the cylinder can be used to stop the end of the test strip 1 placed in the storage pool 9, so as to limit the length of the test strip 1 extending into the storage pool 9, thereby achieving the purpose of rapid chromatographic detection, and also preventing the test strip 1 from moving along the length direction thereof, thereby improving the stability of the test strip 1, facilitating the smooth flow of the reagent in the storage pool 9 along the length direction of the test strip 1, and improving the chromatographic detection effect.

In another embodiment of the present application, please refer to fig. 4 and 9 together, the reagent kit structure 4 further includes a sliding structure for guiding the reagent cup 42 to move in the sleeve 41, the sliding structure includes a sliding block 423 and a sliding slot 411 in sliding fit with the sliding block 423, the sliding block 423 is disposed on the outer wall of the reagent cup 42 in a protruding manner, and the sliding slot 411 is disposed on the inner wall of the sleeve 41 in a recessed manner.

In this embodiment, by providing the sliding structure for guiding the reagent cup 42 to move in the sleeve 41, the sliding structure includes the sliding block 423 and the sliding slot 411 in sliding fit with the sliding block 423, the sliding block 423 is protruded on the outer wall of the reagent cup 42, and the sliding slot 411 is recessed on the inner wall of the sleeve 41, so that in the process that the piston structure 43 pushes the reagent cup 42 to move toward the cavity 23 of the housing 2, under the sliding fit action of the sliding slot 411 and the sliding block 423, not only the stability and reliability of the movement of the reagent cup 42 can be enhanced, but also the friction between the outer wall of the reagent cup 42 and the inner wall of the sleeve 41 can be reduced, thereby facilitating the rapid movement of the reagent cup 42, facilitating the rapid puncture of the closed end 422 of the reagent cup 42 by the column, and improving the detection efficiency.

The above description is only exemplary of the present application and should not be taken as limiting the present application, as any modification, equivalent replacement, or improvement made within the spirit and principle of the present application should be included in the protection scope of the present application.

Claims

1. An immunoassay reagent card, comprising:

the test strip is placed and fixed in the cavity;

the sealing plug is used for sealing the sample adding port; and

2. The immunoassay reagent card of claim 1, wherein a reservoir for holding a reagent is provided in the cavity at a position corresponding to the opening, the opening is in communication with the reservoir, and one end of the strip is disposed in the reservoir.

3. The immunoassay reagent card of claim 2, wherein a bayonet for holding the strip is formed on a side wall of the reservoir, and one end of the strip extends into the reservoir through the bayonet.

4. The immunoassay reagent card of claim 3, wherein the reservoir has a positioning groove at the bottom thereof for receiving and positioning the strip.

5. The immunoassay reagent card of claim 1, wherein said piercing member is a post secured at one end in said cavity, the other end of said post extending into said opening.

6. The immunoassay reagent card of claim 5, wherein the diameter of said column decreases from an end distal to said reagent cup to an end proximal to said reagent cup.

7. The immunoassay reagent card of claim 1, wherein said reagent cartridge structure further comprises a slide structure for guiding said reagent cup to move in said sleeve, said slide structure comprising a slide block and a slide groove slidably engaged with said slide block, said slide block being disposed convexly on an outer wall of said reagent cup, said slide groove being disposed concavely on an inner wall of said sleeve.

8. The immunoassay reagent card of claim 1, wherein the housing comprises a lower shell and an upper cover detachably connected to the lower shell, the sample addition port, the viewing window and the opening are respectively provided on the upper cover, one end of the sleeve is disposed around the opening and fixed on the upper cover, and the upper cover and the lower shell are hermetically connected to form the closed cavity.

9. The immunoassay reagent card of any of claims 1 to 8, wherein the reagent cartridge structure further comprises a piston structure for pushing the reagent cup toward the cavity, the piston structure being slidably mounted in the sleeve, an end of the reagent cup facing away from the closed end being open, an end of the piston structure being blocked on the open end of the reagent cup.

10. The immunoassay reagent card of claim 9, wherein the piston structure comprises a piston body and a push rod connected to the piston body, wherein an end of the piston body distal from an end of the push rod is blocked on the open end of the reagent cup, and an end of the push rod distal from the piston body protrudes from a port of the other end of the sleeve.