CN111424032A - Nucleic acid extraction method, device, system, control equipment and storage medium - Google Patents
Nucleic acid extraction method, device, system, control equipment and storage medium Download PDFInfo
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Abstract
The embodiment of the invention discloses a nucleic acid extraction method, a nucleic acid extraction device, a nucleic acid extraction system, control equipment and a storage medium. The method comprises the steps of obtaining reference point information of nucleic acid extraction equipment, wherein the reference point information comprises the running state and the position information of an operation area of the nucleic acid extraction equipment, determining a current detection task, and obtaining detection parameters of the current detection task and a running script corresponding to the detection parameters, wherein the running script comprises a nucleic acid extraction step, receiving running starting operation, and controlling the nucleic acid extraction equipment to execute the nucleic acid extraction step based on the running starting operation to obtain a nucleic acid extraction result.
Description
Technical Field
The embodiment of the invention relates to a nucleic acid extraction technology, in particular to a nucleic acid extraction method, a nucleic acid extraction device, a nucleic acid extraction system, control equipment and a storage medium.
Background
Nucleic acids are carriers of genetic information and are the primary subjects of most important bioinformatic molecules and biological research. Thus, the extraction of nucleic acids is the most important and basic procedure in molecular biology experiments.
At present, nucleic acid is extracted by adopting a nucleic acid extraction device through a manual experiment mode, but the mode needs multiple devices for matching use, and the nucleic acid extraction step is complicated, the time consumption is long, and the improvement is needed.
Disclosure of Invention
The embodiment of the invention provides a nucleic acid extraction method, a nucleic acid extraction device, a nucleic acid extraction system, control equipment and a storage medium, and aims to improve the efficiency of nucleic acid extraction.
In a first aspect, an embodiment of the present invention provides a nucleic acid extraction method, including:
acquiring reference point information of a nucleic acid extraction device, wherein the reference point information includes an operation state and position information of an operation area of the nucleic acid extraction device;
determining a current detection task, and acquiring detection parameters of the current detection task and an operation script corresponding to the detection parameters, wherein the operation script comprises a nucleic acid extraction step;
receiving a running start operation, and controlling the nucleic acid extraction equipment to execute the nucleic acid extraction step based on the running start operation so as to obtain a nucleic acid extraction result.
In a second aspect, an embodiment of the present invention further provides a nucleic acid extraction apparatus, including:
a reference point information acquisition module for acquiring reference point information of a nucleic acid extraction device, wherein the reference point information includes an operation state and position information of an operation area of the nucleic acid extraction device;
the running script acquisition module is used for determining the current detection task and acquiring the detection parameters of the current detection task and the running script corresponding to the detection parameters, wherein the running script comprises a nucleic acid extraction step;
and the nucleic acid extraction module is used for receiving operation starting operation and controlling the nucleic acid extraction equipment to execute the nucleic acid extraction step based on the operation starting operation so as to obtain a nucleic acid extraction result.
In a third aspect, an embodiment of the present invention further provides a nucleic acid extraction system, including: a control device and a nucleic acid extraction device; wherein the content of the first and second substances,
the control device is used for acquiring reference point information of the nucleic acid extraction device, wherein the reference point information comprises an operation state and position information of an operation area of the nucleic acid extraction device, determining a current detection task, and acquiring a detection parameter of the current detection task and an operation script corresponding to the detection parameter, wherein the operation script comprises a nucleic acid extraction step, receives an operation starting operation, and controls the nucleic acid extraction device to execute the nucleic acid extraction step based on the operation starting operation so as to obtain a nucleic acid extraction result;
the nucleic acid extraction device is used for sending the reference point information, determining detection parameters and extracting nucleic acid to the control device under the control of the control device, and sending the nucleic acid extraction result to the control device.
In a fourth aspect, an embodiment of the present invention further provides a control apparatus, including a memory, a processor, and a computer program stored on the memory and executable on the processor, wherein the processor executes the computer program to implement a method for extracting nucleic acid according to any one of the first aspect.
In a fifth aspect, embodiments of the present invention also provide a storage medium containing computer-executable instructions, wherein the computer-executable instructions, when executed by a computer processor, implement a method for nucleic acid extraction as described in any one of the first aspect.
According to the technical scheme provided by the embodiment of the invention, the reference point information of the nucleic acid extraction equipment is obtained, wherein the reference point information comprises the running state and the position information of the operating area of the nucleic acid extraction equipment, the current detection task is determined, the detection parameters of the current detection task and the running script corresponding to the detection parameters are obtained, the running script comprises a nucleic acid extraction step, the running starting operation is received, the nucleic acid extraction equipment is controlled to execute the nucleic acid extraction step based on the running starting operation, so that the nucleic acid extraction result is obtained, the problems that the nucleic acid extraction step is complicated and the time consumption is long in the manual experiment mode in the prior art are solved, the purposes of scripting the complex flow of the nucleic acid extraction and automatically controlling the nucleic acid extraction equipment to extract the nucleic acid are achieved, and the effect of improving the nucleic acid.
Drawings
FIG. 1 is a schematic flow chart of a method for extracting nucleic acid according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a main interface according to an embodiment of the present invention;
fig. 3 is a schematic diagram of a detection parameter obtaining interface according to an embodiment of the present invention;
FIG. 4 is a schematic flow chart of a method for extracting nucleic acid according to the second embodiment of the present invention;
fig. 5 is a schematic diagram of an operation interface according to a second embodiment of the present invention;
fig. 6 is a schematic diagram of a report display interface according to a second embodiment of the present invention;
FIG. 7 is a schematic structural diagram of a nucleic acid isolation apparatus according to a third embodiment of the present invention;
FIG. 8 is a schematic structural diagram of a nucleic acid extraction system according to a fourth embodiment of the present invention;
fig. 9 is a schematic structural diagram of a control device according to a fifth embodiment of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not limiting of the invention. It should be further noted that, for the convenience of description, only some of the structures related to the present invention are shown in the drawings, not all of the structures.
Example one
FIG. 1 is a schematic flow chart of a nucleic acid extraction method according to an embodiment of the present invention, which can be applied to a case where a nucleic acid extraction device is automatically controlled to extract nucleic acids, and the method can be performed by a nucleic acid extraction apparatus, wherein the apparatus can be implemented by software and/or hardware, and is generally integrated in the control device. Referring specifically to fig. 1, the method may include the steps of:
s110, reference point information of the nucleic acid extraction device is acquired.
Wherein the reference point information may be initialization information of each operation region of the nucleic acid extracting apparatus, including operation state and position information of the operation region of the nucleic acid extracting apparatus. The operation area can comprise a sample feeding area, a waiting area, a purifying area, a detection area, a centrifuge area, a plate rack area and the like.
Alternatively, the initialization of the nucleic acid extraction device may be controlled in accordance with the reset operation and the zero-return operation by receiving the reset operation and the zero-return operation that are externally input, and the reference point information transmitted from the nucleic acid extraction device after the initialization is received.
Explaining the reference point information obtaining process specifically with reference to fig. 2, the control device in this embodiment configures nucleic acid extraction software, the control device receives operation through the nucleic acid extraction software and controls the nucleic acid extraction device to extract nucleic acid, obtains click operation corresponding to a reset button and a return-to-zero button in a main interface of the nucleic acid extraction software, determines to receive corresponding reset operation and return-to-zero operation, and sends a control instruction corresponding to the reset operation and return-to-zero operation to the nucleic acid extraction device, controls initialization of each operation region and obtains operation state and position information of each operation region through a main control board of the nucleic acid extraction device, the main control board of the nucleic acid extraction device controls to send the operation state and position information of each operation region as reference point information to the control device, the control device receives the reference point information, and the main interface pops up feedback information of "reference point information found", the user is reminded that the nucleic acid extraction device has completed initialization so that the user can perform subsequent operations.
And S120, determining the current detection task, and acquiring the detection parameters of the current detection task and the running script corresponding to the detection parameters.
The current detection task may be a nucleic acid extraction project, and the detection parameters of the current detection task may include at least one of a detection name, a subtitle, a sample number, a selection module, a centrifuge rotation speed, and a centrifuge time. Specifically, the control device obtains a click operation corresponding to a newly-built detection button on a main interface of the nucleic acid extraction software, determines a detection task creation instruction corresponding to the click operation, and determines a current detection task according to the detection task creation instruction. Further, the control device pops up a detection parameter acquisition interface shown in fig. 3 according to the determined current detection task, receives externally input detection parameters through the detection parameter acquisition interface, stores the detection parameters, and sends the detection parameters to the nucleic acid extraction device, so that the nucleic acid extraction device can extract nucleic acid according to the detection parameters. Optionally, the test parameter acquisition interface shown in fig. 3 may include one or more test parameter providing options, for example, the selection module may select "centrifuge", "workstation", or "semiconductor cooling", and the sample number may select "24", "48", "72", or "96".
In this embodiment, the control device may determine an execution script based on the detection parameter, and the execution script may include a nucleic acid extraction step. Specifically, after the control device obtains the detection parameters, the control device may determine the operation flows corresponding to the detection parameters, determine the operation sequence of each operation flow, determine the operation sequence as a nucleic acid extraction step, and then correspondingly combine each nucleic acid extraction step with each operation flow to obtain the operation script. And combining the sub-scripts according to the nucleic acid extraction step to obtain the running script of the current nucleic acid extraction project.
S130, receiving the operation starting operation, and controlling the nucleic acid extraction equipment to execute the nucleic acid extraction step based on the operation starting operation to obtain a nucleic acid extraction result.
In this embodiment, after the control device determines the running script, the running script may be sent to the nucleic acid extraction device, the control device obtains a click operation corresponding to the start of running on the main interface, determines the click operation corresponding to the start of running as a running start operation, and sends the running start operation to the control device, and the control device controls the nucleic acid extraction device to execute the nucleic acid extraction step according to the running start operation, so as to extract nucleic acid by the nucleic acid extraction device, and receive a nucleic acid extraction result fed back by the nucleic acid extraction device.
It can be understood that, in the present embodiment, the nucleic acid extraction device is controlled to automatically execute the nucleic acid extraction step of the running script, so that the complex flow of nucleic acid extraction is scripted, and the nucleic acid extraction efficiency is improved. And the control equipment can set a plurality of samples, determine a plurality of running scripts and control the nucleic acid detection equipment to simultaneously or sequentially execute the nucleic acid extraction steps of the running scripts so as to extract nucleic acid, so that human misoperation can be reduced and pollution among the samples can be reduced.
According to the technical scheme provided by the embodiment of the invention, the reference point information of the nucleic acid extraction equipment is obtained, wherein the reference point information comprises the running state and the position information of the operating area of the nucleic acid extraction equipment, the current detection task is determined, the detection parameters of the current detection task and the running script corresponding to the detection parameters are obtained, the running script comprises a nucleic acid extraction step, the running starting operation is received, the nucleic acid extraction equipment is controlled to execute the nucleic acid extraction step based on the running starting operation, so that the nucleic acid extraction result is obtained, the problems that the nucleic acid extraction step is complicated and the time consumption is long in the manual experiment mode in the prior art are solved, the purposes of scripting the complex flow of the nucleic acid extraction and automatically controlling the nucleic acid extraction equipment to extract the nucleic acid are achieved, and the effect of improving the nucleic acid.
Example two
FIG. 4 is a schematic flow chart of a nucleic acid extraction method according to the second embodiment of the present invention. In the technical solution of this embodiment, a new step is added on the basis of the above embodiment, and optionally, the method further includes: if the detection parameter comprises a defined parameter of the fluorescence detection, generating a nucleic acid quantification report based on the defined parameter of the fluorescence detection. In the method, reference is made to the above-described embodiments for those parts which are not described in detail. Referring specifically to fig. 4, the method may include the steps of:
s210, reference point information of the nucleic acid extraction device is acquired.
S220, determining the current detection task, and acquiring the detection parameters of the current detection task and the running script corresponding to the detection parameters.
S230, receiving the operation starting operation, and controlling the nucleic acid extraction equipment to execute the nucleic acid extraction step based on the operation starting operation to obtain a nucleic acid extraction result.
Optionally, the information corresponding to the operation start operation, the information corresponding to the reset operation, the information corresponding to the zeroing operation, and the script file are all sent to the nucleic acid extraction device in a protocol packet form, and the reference point information and the nucleic acid extraction result are sent by the nucleic acid extraction device in the protocol packet form. Specifically, the control device may send information corresponding to the operation start operation, information corresponding to the reset operation, information corresponding to the zeroing operation, the script file, and the like to the nucleic acid extraction device in the form of a protocol packet, so that the nucleic acid extraction device analyzes the protocol packet, controls each operation region to initialize and execute operations such as a nucleic acid extraction step according to the analysis result, and feeds back reference point information and the nucleic acid extraction result to the control device in the form of the protocol packet.
It is understood that, in the course of the control device controlling the nucleic acid extraction device to perform the nucleic acid extraction step, the operation information fed back by the nucleic acid extraction device may be acquired and displayed. In this embodiment, the control device may display the operation information in the following manner: the method comprises the steps of obtaining operation information of the nucleic acid extraction equipment, determining a currently-operated operation area of the nucleic acid extraction equipment according to the operation information, controlling icons corresponding to the currently-operated operation area on an operation page to be displayed in a distinguishing mode, and displaying the operation information, wherein the operation page comprises icons corresponding to the positions of the operation areas in the nucleic acid extraction equipment.
For convenience of understanding, the principle of displaying the operation information is specifically explained with reference to the operation page shown in fig. 5, the interface shown in fig. 5 includes icons corresponding to positions of the operation areas of the nucleic acid extraction device, for example, operation areas such as the well plate area, the purification detection area, the liquid tank and the standby area, and the centrifuge, after the control device obtains the operation information, the operation information may be analyzed, the currently-operating operation area is determined according to the position information in the analysis result, the icon corresponding to the currently-operating operation area is determined on the operation page shown in fig. 5 according to the position corresponding relationship between each operation area and each icon, and the operation information of the currently-operating operation area is displayed through the icon corresponding to the currently-operating operation area. Optionally, the running page may show the running information by flashing different colors on an icon corresponding to the currently running operation area.
S240, if the detection parameters comprise the determination parameters of the fluorescence detection, generating a nucleic acid quantitative report according to the determination parameters of the fluorescence detection.
Specifically, the control device controls the nucleic acid extraction device to perform fluorescence detection during the nucleic acid extraction process according to the determination parameters of the fluorescence detection, obtains the nucleic acid extraction result subjected to the fluorescence detection, generates a nucleic acid quantitative report corresponding to the nucleic acid extraction result subjected to the fluorescence detection according to the determination parameters of the fluorescence detection, and displays the nucleic acid quantitative report through a report display interface shown in fig. 6. Optionally, if the detection parameters do not include the determination parameters of the fluorescence detection, the nucleic acid extraction device does not perform the fluorescence detection during the nucleic acid extraction process, and the control device does not generate a nucleic acid quantitative report after obtaining the nucleic acid extraction result.
Optionally, the control device may draw a graph according to the nucleic acid extraction result, calculate the nucleic acid concentration, and display the graph and the nucleic acid concentration, so as to achieve the purpose of displaying the nucleic acid extraction result through multiple expression modes.
According to the technical scheme provided by the embodiment of the invention, if the detection parameters comprise the determination parameters of the fluorescence detection, the nucleic acid quantitative report is generated according to the determination parameters of the fluorescence detection, so that the purpose of automatically generating the nucleic acid quantitative report according to the nucleic acid extraction result is achieved; and drawing a curve graph according to the nucleic acid extraction result, calculating the nucleic acid concentration, displaying the curve graph and the nucleic acid concentration, and visually displaying the curve graph and the nucleic acid concentration to a user so as to facilitate the subsequent analysis of the user.
EXAMPLE III
FIG. 7 is a schematic structural diagram of a nucleic acid isolation apparatus according to a third embodiment of the present invention. Referring to fig. 7, the apparatus includes: a reference point information acquisition module 31, a running script acquisition module 32, and a nucleic acid extraction module 33.
The reference point information acquiring module 31 is configured to acquire reference point information of a nucleic acid extracting apparatus, where the reference point information includes an operation state and position information of an operation area of the nucleic acid extracting apparatus; the running script obtaining module 32 is configured to determine a current detection task, and obtain a detection parameter of the current detection task and a running script corresponding to the detection parameter, where the running script includes a nucleic acid extraction step; a nucleic acid extraction module 33, configured to receive a run start operation, and control the nucleic acid extraction apparatus to perform the nucleic acid extraction step based on the run start operation, so as to obtain a nucleic acid extraction result.
On the basis of the above technical solutions, the apparatus further includes: a display module; the display module is used for acquiring the operation information of the nucleic acid extraction equipment and determining the current operation area of the nucleic acid extraction equipment according to the operation information;
and controlling icons corresponding to the currently running operation areas on an operation page to be displayed in a distinguishing manner, and displaying the operation information, wherein the operation page comprises icons corresponding to the positions of the operation areas in the nucleic acid extraction equipment.
On the basis of the above technical solutions, the reference point information obtaining module 31 is further configured to receive a reset operation and a zero resetting operation that are input from the outside, control the initialization of the nucleic acid extracting apparatus according to the reset operation and the zero resetting operation, and receive the reference point information sent by the nucleic acid extracting apparatus after the initialization.
On the basis of the above technical solutions, the detection parameters include: at least one of a detection name, a sub-title, a sample number, a selection module, a centrifuge rotation speed, and a centrifugation time.
On the basis of the technical schemes, the reference point information and the nucleic acid extraction result are sent by the nucleic acid extraction equipment in a protocol packet form;
and the information corresponding to the running starting operation, the information corresponding to the resetting operation, the information corresponding to the zeroing operation and the script file are all sent to the nucleic acid extraction equipment in a protocol packet mode.
On the basis of the above technical solutions, the apparatus further includes: a nucleic acid quantitative report generation module; and the nucleic acid quantitative report generating module is used for generating a nucleic acid quantitative report according to the determination parameter of the fluorescence detection if the detection parameter comprises the determination parameter of the fluorescence detection.
On the basis of the above technical solutions, the apparatus further includes: a display module; the display module is used for drawing a curve graph according to the nucleic acid extraction result, calculating the concentration of the nucleic acid and displaying the curve graph and the concentration of the nucleic acid.
According to the technical scheme provided by the embodiment of the invention, the reference point information of the nucleic acid extraction equipment is obtained, wherein the reference point information comprises the running state and the position information of the operating area of the nucleic acid extraction equipment, the current detection task is determined, the detection parameters of the current detection task and the running script corresponding to the detection parameters are obtained, the running script comprises a nucleic acid extraction step, the running starting operation is received, the nucleic acid extraction equipment is controlled to execute the nucleic acid extraction step based on the running starting operation, so that the nucleic acid extraction result is obtained, the problems that the nucleic acid extraction step is complicated and the time consumption is long in the manual experiment mode in the prior art are solved, the purposes of scripting the complex flow of the nucleic acid extraction and automatically controlling the nucleic acid extraction equipment to extract the nucleic acid are achieved, and the effect of improving the nucleic acid.
Example four
FIG. 8 is a schematic structural diagram of a nucleic acid extraction system according to a fourth embodiment of the present invention. Referring to fig. 8, the system includes: a control apparatus 1 and a nucleic acid extraction apparatus 2;
the control device 1 is configured to obtain reference point information of a nucleic acid extraction device, where the reference point information includes an operation state and position information of an operation area of the nucleic acid extraction device, determine a current detection task, and obtain a detection parameter of the current detection task and an operation script corresponding to the detection parameter, where the operation script includes a nucleic acid extraction step, receives an operation start operation, and controls the nucleic acid extraction device to execute the nucleic acid extraction step based on the operation start operation to obtain a nucleic acid extraction result; and the nucleic acid extraction device 2 is used for sending the reference point information, the determined detection parameters and the extracted nucleic acid to the control device under the control of the control device and sending the nucleic acid extraction result to the control device.
On the basis of the above technical solutions, the control device 1 is further configured to obtain operation information of the nucleic acid extraction device, and determine a currently operating operation region of the nucleic acid extraction device according to the operation information;
and controlling icons corresponding to the currently running operation areas on an operation page to be displayed in a distinguishing manner, and displaying the operation information, wherein the operation page comprises icons corresponding to the positions of the operation areas in the nucleic acid extraction equipment.
On the basis of the above technical solutions, the control device 1 is further configured to receive an externally input reset operation and a zero-resetting operation, control the initialization of the nucleic acid extraction device according to the reset operation and the zero-resetting operation, and receive the reference point information sent by the initialized nucleic acid extraction device.
On the basis of the above technical solutions, the detection parameters include: at least one of a detection name, a sub-title, a sample number, a selection module, a centrifuge rotation speed, and a centrifugation time.
On the basis of the technical schemes, the reference point information and the nucleic acid extraction result are sent by the nucleic acid extraction equipment in a protocol packet form;
and the information corresponding to the running starting operation, the information corresponding to the resetting operation, the information corresponding to the zeroing operation and the script file are all sent to the nucleic acid extraction equipment in a protocol packet mode.
On the basis of the above technical solutions, the control device 1 is further configured to generate a nucleic acid quantitative report according to the determination parameter of the fluorescence detection if the detection parameter includes the determination parameter of the fluorescence detection.
On the basis of the above-described respective technical solutions, the control apparatus 1 is further configured to draw a graph according to the nucleic acid extraction result, calculate a nucleic acid concentration, and display the graph and the nucleic acid concentration.
EXAMPLE five
Fig. 9 is a schematic structural diagram of a control device according to a fifth embodiment of the present invention. Referring to FIG. 9, FIG. 9 illustrates a block diagram of an exemplary control device 12 suitable for use in implementing embodiments of the present invention. The control device 12 shown in fig. 9 is only an example, and should not bring any limitation to the functions and the scope of use of the embodiment of the present invention.
As shown in fig. 9, the control device 12 is in the form of a general purpose computing device. The components of the control device 12 may include, but are not limited to: one or more processors or processing units 16, a system memory 28, and a bus 18 that couples various system components including the system memory 28 and the processing unit 16.
The system memory 28 may include computer system readable media in the form of volatile memory, such as Random Access Memory (RAM)30 and/or cache memory 32. The control device 12 may further include other removable/non-removable, volatile/nonvolatile computer system storage media. By way of example only, storage system 34 may be used to read from and write to non-removable, nonvolatile magnetic media (not shown in FIG. 9, and commonly referred to as a "hard drive"). Although not shown in FIG. 9, a magnetic disk drive for reading from and writing to a removable, nonvolatile magnetic disk (e.g., a "floppy disk") and an optical disk drive for reading from or writing to a removable, nonvolatile optical disk (e.g., a CD-ROM, DVD-ROM, or other optical media) may be provided. In these cases, each drive may be connected to bus 18 by one or more data media interfaces. The memory 28 may include at least one program product having a set of program modules (e.g., a reference point information acquisition module 31, a running script acquisition module 32, and a nucleic acid extraction module 33 of a nucleic acid extraction device) configured to perform the functions of embodiments of the present invention.
A program/utility 44 having a set of program modules 46 (e.g., reference point information acquisition module 31, run script acquisition module 32, and nucleic acid extraction module 33 of a nucleic acid extraction device) may be stored, for example, in memory 28, such program modules 46 including, but not limited to, an operating system, one or more application programs, other program modules, and program data, each of which examples or some combination thereof may include an implementation of a network environment. Program modules 46 generally carry out the functions and/or methodologies of the described embodiments of the invention.
The control device 12 may also communicate with one or more external devices 14 (e.g., keyboard, pointing device, display 24, etc.), and may also communicate with one or more devices that enable a user to interact with the control device 12, and/or with any devices (e.g., network card, modem, etc.) that enable the control device 12 to communicate with one or more other computing devices, such communication may occur via input/output (I/O) interfaces 22. moreover, the control device 12 may also communicate with one or more networks (e.g., local area network (L AN), Wide Area Network (WAN) and/or a public network, such as the Internet) via a network adapter 20. As shown, the network adapter 20 communicates with other modules of the control device 12 via the bus 18. it should be appreciated that, although not shown, other hardware and/or software modules may be used in conjunction with the control device 12, including, but not limited to, microcode, device drivers, redundant processing units, external disk drive arrays, RAID systems, tape drives, data backup storage systems, and the like.
The processing unit 16 executes various functional applications and data processing by executing programs stored in the system memory 28, for example, to implement a nucleic acid extraction method provided by an embodiment of the present invention, the method including:
acquiring reference point information of a nucleic acid extraction device, wherein the reference point information includes an operation state and position information of an operation area of the nucleic acid extraction device;
determining a current detection task, and acquiring detection parameters of the current detection task and an operation script corresponding to the detection parameters, wherein the operation script comprises a nucleic acid extraction step;
receiving a running start operation, and controlling the nucleic acid extraction equipment to execute the nucleic acid extraction step based on the running start operation so as to obtain a nucleic acid extraction result.
The processing unit 16 executes various functional applications and data processing by executing programs stored in the system memory 28, for example, to implement a nucleic acid extraction method provided by the embodiment of the present invention.
Of course, those skilled in the art will understand that the processor may also implement the technical solution of the method for extracting nucleic acid provided in any embodiment of the present invention.
EXAMPLE six
An embodiment of the present invention further provides a computer-readable storage medium, on which a computer program is stored, where the computer program, when executed by a processor, implements a method for extracting nucleic acid according to an embodiment of the present invention, the method including:
acquiring reference point information of a nucleic acid extraction device, wherein the reference point information includes an operation state and position information of an operation area of the nucleic acid extraction device;
determining a current detection task, and acquiring detection parameters of the current detection task and an operation script corresponding to the detection parameters, wherein the operation script comprises a nucleic acid extraction step;
receiving a running start operation, and controlling the nucleic acid extraction equipment to execute the nucleic acid extraction step based on the running start operation so as to obtain a nucleic acid extraction result.
Of course, the computer program stored on the computer-readable storage medium provided by the embodiments of the present invention is not limited to the above method operations, and may also perform related operations in a nucleic acid extraction method provided by any embodiments of the present invention.
Computer storage media for embodiments of the invention may employ any combination of one or more computer-readable media. The computer readable medium may be a computer readable signal medium or a computer readable storage medium. A computer readable storage medium may be, for example, but not limited to, an electronic, magnetic, optical, electromagnetic, infrared, or semiconductor system, or device, or any combination of the foregoing. More specific examples (a non-exhaustive list) of the computer readable storage medium would include the following: an electrical connection having one or more wires, a portable computer diskette, a hard disk, a Random Access Memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or flash memory), an optical fiber, a portable compact disc read-only memory (CD-ROM), an optical storage device, a magnetic storage device, or any suitable combination of the foregoing. In the context of this document, a computer readable storage medium may be any tangible medium that can contain, or store a program for use by or in connection with an instruction execution system, or device.
A computer readable signal medium may include reference point information, a running script, etc., having computer readable program code embodied therein. Such propagated reference point information, run script, etc. A computer readable signal medium may also be any computer readable medium that is not a computer readable storage medium and that can communicate, propagate, or transport a program for use by or in connection with an instruction execution system, or device.
Program code embodied on a computer readable medium may be transmitted using any appropriate medium, including but not limited to wireless, wireline, optical fiber cable, RF, etc., or any suitable combination of the foregoing.
Computer program code for carrying out operations for aspects of the present invention may be written in any combination of one or more programming languages, including AN object oriented programming language such as Java, Smalltalk, C + +, and conventional procedural programming languages, such as the "C" programming language or similar programming languages.
It should be noted that, in the embodiment of the nucleic acid extracting apparatus, the modules included are merely divided according to the functional logic, but are not limited to the above division as long as the corresponding functions can be realized; in addition, specific names of the functional units are only for convenience of distinguishing from each other, and are not used for limiting the protection scope of the present invention.
It is to be noted that the foregoing is only illustrative of the preferred embodiments of the present invention and the technical principles employed. It will be understood by those skilled in the art that the present invention is not limited to the particular embodiments described herein, but is capable of various obvious changes, rearrangements and substitutions as will now become apparent to those skilled in the art without departing from the scope of the invention. Therefore, although the present invention has been described in greater detail by the above embodiments, the present invention is not limited to the above embodiments, and may include other equivalent embodiments without departing from the spirit of the present invention, and the scope of the present invention is determined by the scope of the appended claims.
Claims (11)
1. A method for extracting nucleic acid, comprising:
acquiring reference point information of a nucleic acid extraction device, wherein the reference point information includes an operation state and position information of an operation area of the nucleic acid extraction device;
determining a current detection task, and acquiring detection parameters of the current detection task and an operation script corresponding to the detection parameters, wherein the operation script comprises a nucleic acid extraction step;
receiving a running start operation, and controlling the nucleic acid extraction equipment to execute the nucleic acid extraction step based on the running start operation so as to obtain a nucleic acid extraction result.
2. The method of claim 1, further comprising:
acquiring operation information of the nucleic acid extraction equipment, and determining a currently operated operation area of the nucleic acid extraction equipment according to the operation information;
and controlling icons corresponding to the currently running operation areas on an operation page to be displayed in a distinguishing manner, and displaying the operation information, wherein the operation page comprises icons corresponding to the positions of the operation areas in the nucleic acid extraction equipment.
3. The method of claim 1, wherein the obtaining reference point information for the nucleic acid extraction device comprises:
receiving reset operation and zero operation input from outside, controlling the nucleic acid extraction device to initialize according to the reset operation and the zero operation, and receiving the reference point information sent by the initialized nucleic acid extraction device.
4. The method of claim 1, wherein detecting the parameter comprises: at least one of a detection name, a sub-title, a sample number, a selection module, a centrifuge rotation speed, and a centrifugation time.
5. The method according to claim 1, wherein the reference point information and the nucleic acid extraction result are transmitted by the nucleic acid extraction device in a protocol packet form;
and the information corresponding to the running starting operation, the information corresponding to the resetting operation, the information corresponding to the zeroing operation and the script file are all sent to the nucleic acid extraction equipment in a protocol packet mode.
6. The method of claim 1, further comprising:
if the detection parameter comprises a defined parameter of the fluorescence detection, generating a nucleic acid quantification report based on the defined parameter of the fluorescence detection.
7. The method of claim 1, further comprising:
drawing a graph according to the nucleic acid extraction result, calculating the concentration of the nucleic acid, and displaying the graph and the concentration of the nucleic acid.
8. A nucleic acid extraction device, comprising:
a reference point information acquisition module for acquiring reference point information of a nucleic acid extraction device, wherein the reference point information includes an operation state and position information of an operation area of the nucleic acid extraction device;
the running script acquisition module is used for determining the current detection task and acquiring the detection parameters of the current detection task and the running script corresponding to the detection parameters, wherein the running script comprises a nucleic acid extraction step;
and the nucleic acid extraction module is used for receiving operation starting operation and controlling the nucleic acid extraction equipment to execute the nucleic acid extraction step based on the operation starting operation so as to obtain a nucleic acid extraction result.
9. A nucleic acid extraction system, comprising: a control device and a nucleic acid extraction device; wherein the content of the first and second substances,
the control device is used for acquiring reference point information of the nucleic acid extraction device, wherein the reference point information comprises an operation state and position information of an operation area of the nucleic acid extraction device, determining a current detection task, and acquiring a detection parameter of the current detection task and an operation script corresponding to the detection parameter, wherein the operation script comprises a nucleic acid extraction step, receives an operation starting operation, and controls the nucleic acid extraction device to execute the nucleic acid extraction step based on the operation starting operation so as to obtain a nucleic acid extraction result;
the nucleic acid extraction device is used for sending the reference point information, determining detection parameters and extracting nucleic acid to the control device under the control of the control device, and sending the nucleic acid extraction result to the control device.
10. A control apparatus comprising a memory, a processor and a computer program stored on the memory and executable on the processor, wherein the processor implements a method of nucleic acid extraction as claimed in any one of claims 1 to 7 when executing the computer program.
11. A storage medium containing computer-executable instructions which, when executed by a computer processor, implement a method of nucleic acid extraction as claimed in any one of claims 1 to 7.
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