CN111272639B - Fluorescent particle detection device and method - Google Patents
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- 238000001514 detection method Methods 0.000 title claims abstract description 111
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- 238000012360 testing method Methods 0.000 claims abstract description 36
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G01N2015/1022—
Abstract
The invention provides a fluorescent particle detection device and a method, wherein the device comprises: a laser source for emitting laser light; the wavelength selection unit is connected with the laser source and used for dynamically adjusting the wavelength of the laser emitted by the laser source; the focusing unit is connected with the laser source and is used for focusing the laser into sheet light; the polarizing unit is connected with the focusing unit and is used for modulating the polarization state of the sheet light; the particle testing area is arranged behind the polarizing unit and is used for the air sample to be tested to pass through; the detection unit comprises detection subunits which are symmetrically arranged on the periphery of the particulate matter test area and are used for detecting the polarized scattered light intensity information and the fluorescence intensity information of different angles of the particulate matters in the air sample to be detected flowing through the particulate matter test area after the particulate matters scatter the laser; the calculation processing unit and the spectrum demodulation unit are connected with the detection unit and are respectively used for analyzing the fluorescence characteristics and the polarization characteristics of the particles and demodulating the spectrum to obtain spectrum information. The preliminary fluorescence classification information and more detailed spectral information can be obtained.
Description
Technical Field
The invention relates to the technical field of particle detection, in particular to a fluorescent particle detection device and method.
Background
Atmospheric particulates have increasingly severe influence on the environment, are complex in components and diverse in source, and researchers need to perform discriminant analysis on the source of the particulates according to the physical and chemical characteristics of the particulates. Most of the atmospheric particulates capable of generating fluorescence show high toxicity, and the identification of the fluorescent particulates can provide clues for researching the types and sources of the fluorescent particulates and is of great help for researching the characteristics of the particulates. As shown in fig. 1, when a substance having a fluorescent functional group is irradiated with an excitation light source, according to the explanation of energy level transition, when the fluorescent substance is excited, the substance molecule absorbs photons of a characteristic frequency, and transitions from the original ground state energy level to each of different vibration energy levels of an excited electronic state. The molecules in the excited state are unstable, and after vibrational relaxation, the molecules return to the lowest vibrational level of the first excited electronic state, and then the electrons transition to any vibrational level of the ground state, releasing energy and emitting light with a wavelength longer than the wavelength of the incident light, i.e., fluorescence.
The fluorescence analysis method generally adopts a standard working curve method, takes various known amounts of fluorescent substances to prepare a series of standard solutions, measures the fluorescence intensity of the standard solutions, and then gives a working curve of the fluorescence intensity to the concentration of the standard solutions. Under the same instrument condition, the fluorescence intensity of the unknown sample is measured, and then the concentration (content) of the unknown sample is checked from the standard working curve. However, in the case of a sample, the concentration of an unknown sample in a solution is detected, the fluorescence property of a single particle is difficult to determine, and due to the limitation of a chemical method, the detection speed is slow, and the method has the defects of non-real-time and ex-situ measurement. The number of parts that need to be manually operated is high and the cost is high. The method has difficulty in realizing a rapid dynamic single-particle detection process for the fluorescence of the atmospheric particulates.
The above background disclosure is only provided to assist understanding of the concept and technical solution of the present invention, which does not necessarily belong to the prior art of the present patent application, and should not be used to evaluate the novelty and inventive step of the present application in the case that there is no clear evidence that the above content is disclosed at the filing date of the present patent application.
Disclosure of Invention
The invention provides a fluorescent particle detection device and method, aiming at solving the problems of slow detection, non-dynamic detection and non-single particle detection of a chemical fluorescence analysis method in the prior art.
In order to solve the above problems, the technical solution adopted by the present invention is as follows:
a fluorescent particle detection device comprises a laser source, a wavelength selection unit, a focusing unit, a polarizing unit, a particle test area, a detection unit, a calculation processing unit and a spectrum demodulation unit; the laser source is used for emitting laser; the wavelength selection unit is connected with the laser source and used for dynamically adjusting the wavelength of the laser emitted by the laser source; the focusing unit is connected with the laser source and is used for focusing the laser into sheet light; the polarizing unit is connected with the focusing unit and is used for modulating the polarization state of the sheet light; the particle testing area is arranged behind the polarizing unit and is used for the air sample to be tested to pass through; the detection unit comprises detection subunits which are symmetrically arranged on the periphery of the particulate matter test area and used for detecting the polarized scattered light intensity information of the particulate matter in the air sample to be detected flowing through the particulate matter test area at different angles after the particulate matter scatters the laser and the fluorescence intensity information of the particulate matter in the air sample to be detected at different angles after the particulate matter is excited by the laser; the calculation processing unit is connected with the detection unit and is used for analyzing the fluorescence characteristics and polarization characteristics of the particles; the spectrum demodulation unit is connected with the detection unit and used for spectrum demodulation to obtain spectrum information, and different air samples to be detected are distinguished by using different spectrum information.
Preferably, the laser source is a multi-wavelength laser; the focusing unit is a cylindrical mirror; the polarizing unit is a polarizing plate; the detection unit uses an optical fiber to conduct signals to the photomultiplier tube to detect optical signals.
Preferably, the cross section of the detection subunit is square, and comprises 4 polarization information detection areas and 1 fluorescence information detection area.
Preferably, the fluorescence information detection region is located in the middle of the square, the polarization information detection regions are uniformly arranged on the periphery, and the polarization detection angles of the polarization information detection regions are different.
Preferably, the polarization information detection area corresponds to a scattering detection channel, and the scattering exhibited by the fluorescent particles and the non-fluorescent particles of the particulate matter accords with the pulse intensity of the effective particles;
the fluorescence information detection area corresponds to the fluorescence detection channel, and fluorescence passes through the optical filter when the fluorescence information detection area conforms to the cut-off wavelength range.
Preferably, the particle testing area is circular, and the air sample to be tested vertically passes through the center of the particle testing area.
Preferably, the spectrum demodulation unit includes a spectrometer, and the spectrum information includes intensity information and wavelength information of the optical signal.
The invention also provides a fluorescent particle detection method, which comprises the following steps: s1: controlling the air sample to be tested to pass through the particulate matter testing area at a constant flow rate; s2: controlling laser with determined emission wavelength to irradiate the air sample to be detected after focusing and polarizing; s3: controlling and detecting the polarized scattered light intensity information of different angles of the particles in the air sample to be detected after the particles scatter laser and the fluorescence intensity information of different angles of the particles after the particles excite the laser; s4: analyzing the fluorescence characteristics and polarization characteristics of the particles; s5: and controlling spectrum demodulation to obtain spectrum information, and distinguishing different air samples to be detected by utilizing different spectrum information.
Preferably, the particle testing area is circular, and the air sample to be tested vertically passes through the center of the particle testing area.
Preferably, analyzing the fluorescence and polarization characteristics of the particulate matter comprises:
and calculating the ratio of the light intensity signals of the scattered light and the light intensity signals of the fluorescence at different angles to obtain the fluorescence characteristics of the particles, and judging whether the particles are particles capable of generating fluorescence or not.
The invention has the beneficial effects that: the utility model provides a fluorescent particle detection device and method, the sample that awaits measuring is shone through the laser after polarization treatment, survey its polarization scattered light intensity of different angles and the excitation fluorescence light intensity of different angles again and obtain preliminary fluorescence classified information through asking the fluorescence light intensity and the polarization scattered light intensity ratio of different angles, and realize current spectrum demodulation through spectrum demodulation unit, thereby obtain more careful spectral information, utilize preliminary fluorescence classified information can judge whether the particulate matter is sent fluorescence, and more careful spectral information can obtain the more careful differentiation of fluorescent particle.
Further, fluorescent detection arms with different cut-off wavelengths are selected so as to obtain more detailed fluorescent information; the device is purely automatically operated by instruments after selecting the basic components, the identification and classification work of the fluorescent particles is not required to be manually realized, the device is easy to modify, the cost is well controlled, and the dynamic and rapid single particle detection of the fluorescent particles is realized.
Drawings
Fig. 1 is a schematic diagram of energy level transitions in an embodiment of the present invention.
FIG. 2 is a schematic diagram of a fluorescent particle detection device according to an embodiment of the present invention.
FIG. 3 is a schematic cross-sectional view of a probe subunit in an embodiment of the invention.
FIG. 4 is a schematic diagram of a fluorescent particle detection method according to an embodiment of the present invention.
FIG. 5 is a comparison of fluorescent particles in a scattering channel and a fluorescent channel in an embodiment of the invention.
FIG. 6 is a comparison of non-fluorescent particles in a scattering channel and a fluorescent channel in an embodiment of the invention.
FIG. 7 is a representation of an orange fluorescent sphere (emission wavelength 580 nm) and a red fluorescent sphere (emission wavelength 610 nm) mixed with a non-fluorescent PSL bead in a 10 degree forward scattering, 115 degree 550nm long-wavelength pass detection fluorescence channel and 115 degree 600nm long-wavelength pass detection fluorescence channel in an embodiment of the invention.
Detailed Description
In order to make the technical problems, technical solutions and advantageous effects to be solved by the embodiments of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and the embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element. In addition, the connection may be for either a fixing or a circuit communication.
It is to be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in an orientation or positional relationship indicated in the drawings for convenience in describing the embodiments of the present invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed in a particular orientation, and be in any way limiting of the present invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the embodiments of the present invention, "a plurality" means two or more unless specifically limited otherwise.
As shown in fig. 2, the present invention provides a fluorescent particle detection device, which includes a laser source, a wavelength selection unit, a focusing unit, a polarizing unit, a particle testing area, a detection unit, a calculation processing unit and a spectrum demodulation unit;
a laser source 1 for emitting laser light;
the wavelength selection unit 2 is connected with the laser source 1 and used for dynamically adjusting the wavelength of the laser emitted by the laser source 1;
the focusing unit 3 is connected with the laser source 1 and used for focusing the laser into sheet light;
the polarizing unit 4 is connected with the focusing unit 3 and is used for modulating the polarization state of the sheet light;
the particle testing area 5 is arranged behind the polarizing unit 4 and is used for the air sample to be tested to pass through;
the detection unit comprises detection subunits 6 symmetrically arranged around the particulate matter test area and is used for detecting the polarized scattered light intensity information of different angles of the particulate matter in the air sample to be tested flowing through the particulate matter test area 5 after the particulate matter scatters the laser and the fluorescence intensity information of different angles after the particulate matter is excited by the laser;
the calculation processing unit 7 is connected with the detection unit and is used for analyzing the fluorescence characteristics and the polarization characteristics of the particles;
the spectrum demodulation unit is located at the same position as the calculation processing unit and is not shown in the figure. And the detection unit is connected with the detection unit and used for spectrum demodulation to obtain spectrum information, and different air samples to be detected are distinguished by using different spectrum information.
Through the device, an air sample to be tested flows through the particulate matter test area 5 at a constant speed, laser emitted by the laser source 1 sequentially passes through the wavelength selection unit 2 to determine the emergent wavelength, the focusing unit 3 focuses the laser, and the polarizing unit 4 polarizes the laser to irradiate the particulate matter test area 5; the detection unit is used for detecting the scattered light intensity and the fluorescence intensity of the particles in the air sample to be detected flowing through the particle test area after scattering the laser; the calculation processing unit 7 calculates to obtain preliminary fluorescence characteristic information according to the ratio of the polarized scattered light and the excited fluorescence detected by the detection unit, analyzes the preliminary fluorescence characteristic information to obtain the preliminary fluorescence characteristic of the current particulate matter, and judges whether the particulate matter is the particulate matter capable of generating fluorescence; the spectrum demodulation unit can realize current spectrum demodulation by means of the spectrometer, so that more detailed spectrum information can be obtained, and different samples can be distinguished by using different spectrum information. The angle and the number of the detection subunits can be adjusted according to the experimental requirements.
In one embodiment of the invention, the laser source is a multi-wavelength laser; the focusing unit is a cylindrical mirror; the polarizing unit is a polarizing plate; the detection unit uses optical fiber to transmit signals to the photomultiplier to detect optical signals, detection units with various angles can be additionally used, and filters with different wavelengths can be selected to detect fluorescent signals with different wavelengths. The wavelength selection unit is connected with the laser source to select the wavelength, and the laser source emits laser with given wavelength, the laser is focused by the focusing unit and then is irradiated to the particle passing point in the particle testing area after being polarized by the polarizing unit
As shown in fig. 3, the detection subunit has a square cross section and includes 4 polarization information detection regions 8 and 1 fluorescence information detection region 9.
The fluorescence information detection area 9 is located in the middle of the square, the polarization information detection areas 8 are uniformly arranged on the periphery, and the polarization detection angles of the polarization information detection areas are different. The polarization information detection area 8 corresponds to a scattering detection channel, and the scattering shown by fluorescent particles and non-fluorescent particles of the particulate matters accords with the pulse intensity of effective particles; the fluorescence information detection area corresponds to the fluorescence detection channel, and fluorescence passes through the optical filter when the fluorescence information detection area conforms to the cut-off wavelength range.
In one embodiment of the invention, the particle testing area is circular, and the air sample to be tested vertically passes through the center of the particle testing area, and is detected by the detection unit through scattering signals generated by the laser.
As shown in fig. 4, the present invention provides a fluorescent particle detection method, comprising the following steps:
s1: controlling the air sample to be tested to pass through the particulate matter testing area at a constant flow rate;
s2: controlling laser with determined emission wavelength to irradiate the air sample to be detected after focusing and polarizing;
s3: controlling and detecting the polarized scattered light intensity information of different angles of the particles in the air sample to be detected after the particles scatter laser and the fluorescence intensity information of different angles of the particles after the particles excite the laser;
s4: analyzing the fluorescence characteristics and polarization characteristics of the particles;
s5: and controlling spectrum demodulation to obtain spectrum information, and distinguishing different air samples to be detected by utilizing different spectrum information.
In one embodiment of the present invention, analyzing the fluorescence and polarization characteristics of the particulate matter comprises:
and calculating the ratio of the light intensity signals of the scattered light and the light intensity signals of the fluorescence at different angles to obtain the fluorescence characteristics of the particles, and judging whether the particles are particles capable of generating fluorescence or not.
After spectrum demodulation, the fluorescence information can be expanded into spectrum information containing wavelength information only from the intensity information, and different samples can be distinguished by using different spectrum information.
The method comprises the steps of enabling a sample to be detected to flow through a particle testing area at a constant flow rate, enabling laser to irradiate the particle testing area after the emission wavelength is determined by a wavelength selection unit and is subjected to focusing polarization treatment, detecting polarized scattered light intensity information of different angles of the current particles after the current particles scatter laser light and fluorescence intensity information of different angles of the current particles after the current particles are excited by the laser light, and measuring a light intensity signal of scattered light emitted at a certain scattering angle and a light intensity signal of fluorescence, so that the fluorescence property of the particles which cannot be measured by a traditional light scattering method can be obtained, and preliminarily extracting the fluorescence characteristic of the particles according to the ratio of the light intensity signal of the fluorescence to the light intensity signal of the scattered light.
Firstly, selecting the simplest fluorescence detection mode, enabling an air sample to be detected to flow through a test area at a constant speed, and irradiating the test area after laser is subjected to focusing and polarization treatment. By utilizing the difference and the same point of the fluorescent particles and the non-fluorescent particles, in the scattering detection channel, the scattering shown by the fluorescent particles and the non-fluorescent particles accords with the pulse intensity of effective particles, and in the fluorescent detection channel, because of the limitation of the cut-off wavelength of the detection channel, the scattered light can not pass through the fluorescent detection channel, and the fluorescent light can pass through the optical filter when the fluorescent light accords with the cut-off wavelength range, so that the difference between the fluorescent particles and the non-fluorescent particles can be obviously seen in the fluorescent channel. To simplify the experiment, in a feasibility experiment for exploring fluorescence detection, all polarizers and wave plates in front of the detection channel were removed, and only the light intensity information was considered and the polarization information was not considered for the moment.
Fig. 5 and fig. 6 show that the fluorescent particles and the non-fluorescent particles respectively have intensity differences in a 115-degree scattering channel and a 115-degree fluorescence channel, which are similar in scattering channel, but have significant differences in fluorescence detection channel, which proves that it is feasible to distinguish whether the particles have fluorescence property by using fluorescence scattering, and provides feasibility for multiple property analysis of the particles.
On the basis of distinguishing single fluorescence particulate matter and non-fluorescence particulate matter respectively, carry out three sets of semi-quantitative experiments with two kinds of material mixing experiments, the contrast, A experiment is 100% non-fluorescence particulate matter, and the B experiment is for mixing some fluorescence particulate matter, and the C experiment is 100% fluorescence particulate matter, contrasts the intensity of scattering channel and fluorescence channel respectively. Because the scattered signal intensities of the fluorescent particles and the non-fluorescent particles are similar, whether the particles are fluorescent particles or not is judged according to the ratio of the fluorescent signals to the non-fluorescent signals. Two different fluorescent spheres behave similarly in the fluorescent channel under detection at the respective appropriate detection channel cut-off wavelength. Two fluorescent spheres with different emission wavelengths and non-fluorescent PSL spheres are mixed, the cutoff wavelength of one 115-degree detection unit is set to be 550nm, the cutoff wavelength of the other 115-degree detection unit which is symmetrical to the detection unit is set to be 600nm, and mixed detection of double fluorescent channels and scattering channels is achieved.
FIG. 7 is a representation of a mixture of orange fluorescent spheres (emission wavelength 580 nm) and red fluorescent spheres (610 nm) with non-fluorescent PSL beads in a 10 degree forward scatter, 115 degree 550nm long pass detection fluorescence channel and 115 degree 600nm long pass detection fluorescence channel.
The left y-axis of FIG. 7 corresponds to the 10 degree forward scatter values, the right y-axis corresponds to the 115 degree 550nm and 600nm fluorescence detection channels, the dashed line corresponds to the 10 degree forward scatter, the light gray corresponds to the 550nm fluorescence detection channel, and the black corresponds to the 600nm fluorescence detection channel. Comparing the three images, it is clear that the difference of the dual fluorescence channels can be seen, and then different particles can be distinguished. For the first box on the left, PSL beads were identified, which have very weak values in both fluorescence channels; the middle frame identifies an orange fluorescent sphere, the values of the two fluorescent channels differ significantly because only one filter matches the emission wavelength; the rightmost box identifies the red fluorescent sphere, which is relatively strong in both fluorescent channels. Thus, the device can identify approximate fluorescence intervals of two fluorescent spheres and distinguish another non-fluorescent sphere.
Up to this point, the distinction between fluorescent particles and non-fluorescent particles, two different fluorescent particles, has been accomplished. And when they are mixed two by two, different fluorescent particles can be identified from the mixed pulse in a semi-quantitative manner.
According to the fluorescent particle detection device, the laser after polarization treatment is used for irradiating a sample to be detected, then the polarized scattered light intensities of different angles and the excited fluorescent light intensities of different angles of the sample are detected, the ratio of the fluorescent light intensities of different angles to the polarized scattered light intensities is obtained to obtain preliminary fluorescent classification information, the current spectrum demodulation is realized through the spectrum demodulation unit, so that more detailed spectrum information is obtained, whether the particles emit fluorescence or not can be judged by using the preliminary fluorescent classification information, and the more detailed spectrum information can be used for more finely distinguishing the fluorescent particles. The device can select the fluorescent detection arms with different cut-off wavelengths so as to obtain more detailed fluorescent information; the device is purely automatically operated by instruments after selecting the basic components, the identification and classification work of the fluorescent particles is not required to be manually realized, the device is easy to modify, the cost is well controlled, and the dynamic and rapid single particle detection of the fluorescent particles is realized.
The foregoing is a further detailed description of the invention in connection with specific preferred embodiments and it is not intended to limit the invention to the specific embodiments described. For those skilled in the art to which the invention pertains, several equivalent substitutions or obvious modifications can be made without departing from the spirit of the invention, and all the properties or uses are considered to be within the scope of the invention.
Claims (10)
1. A fluorescent particle detection device is characterized by comprising a laser source, a wavelength selection unit, a focusing unit, a polarizing unit, a particle test area, a detection unit, a calculation processing unit and a spectrum demodulation unit;
the laser source is used for emitting laser;
the wavelength selection unit is connected with the laser source and used for dynamically adjusting the wavelength of the laser emitted by the laser source;
the focusing unit is connected with the laser source and is used for focusing the laser into sheet light;
the polarizing unit is connected with the focusing unit and is used for modulating the polarization state of the sheet light;
the particle testing area is arranged behind the polarizing unit and is used for the air sample to be tested to pass through;
the detection unit comprises detection subunits symmetrically arranged around the particulate matter test area, each detection subunit comprises a polarization information detection area and a fluorescence information detection area, and polarization detection angles of the polarization information detection areas are different and are used for detecting polarized scattered light intensity information of particulate matters in the air sample to be detected flowing through the particulate matter test area, which are scattered by laser light at different angles, and fluorescence intensity information of the particulate matters after being excited by the laser light at different angles;
the calculation processing unit is connected with the detection unit and is used for analyzing the fluorescence characteristics and the polarization characteristics of the particles and judging whether the particles are particles capable of generating fluorescence or not;
the spectrum demodulation unit is connected with the detection unit and used for spectrum demodulation to obtain spectrum information, and different air samples to be detected are distinguished by using different spectrum information;
thereby can use the laser irradiation sample that awaits measuring after the polarization is handled, detect its polarization scattered light intensity of different angles and the excitation fluorescence light intensity of different angles again, obtain preliminary fluorescence classification information through the fluorescence light intensity of asking different angles and polarization scattered light intensity ratio, and realize current spectrum demodulation through spectrum demodulation unit, thereby obtain more careful spectral information, whether utilize preliminary fluorescence classification information to judge the particulate matter and sent fluorescence, utilize more careful spectral information to obtain the more careful differentiation of fluorescent particulate matter, utilize the different samples that distinguish of spectral information, realize the mixed detection of two fluorescence channels and scattering channel, multiple attribute analysis for the particulate matter provides the feasibility.
2. The fluorescent particle detection device of claim 1,
the laser source is a multi-wavelength laser;
the focusing unit is a cylindrical mirror;
the polarizing unit is a polarizing plate;
the detection unit uses an optical fiber to conduct signals to the photomultiplier tube to detect optical signals.
3. The fluorescent particle detection device of claim 1, wherein the detection subunit is square in cross section and includes 4 polarized information detection zones and 1 fluorescent information detection zone.
4. The fluorescent particle detector of claim 3, wherein the fluorescent information detection region is located in the middle of the square and the polarized information detection region is uniformly arranged around the square.
5. The fluorescent particle detector of claim 4, wherein the polarized information detection region corresponds to a scatter detection channel, and the fluorescent particles and non-fluorescent particles of the particles exhibit scatter consistent with the pulse intensity of the effective particles;
the fluorescence information detection area corresponds to the fluorescence detection channel, and fluorescence passes through the optical filter when the fluorescence information detection area conforms to the cut-off wavelength range.
6. The fluorescent particulate detection device of claim 1, wherein the particulate test zone is circular and the air sample to be tested passes vertically through the center of the particulate test zone.
7. The fluorescent particle detection device of claim 1, wherein the spectrum demodulation unit comprises a spectrometer, and the spectrum information comprises intensity information and wavelength information of the optical signal.
8. A fluorescent particle detection method, using the fluorescent particle detection device according to any one of claims 1 to 7, comprising the steps of:
s1: controlling the air sample to be tested to pass through the particulate matter testing area at a constant flow rate;
s2: controlling laser with determined emission wavelength to irradiate the air sample to be detected after focusing and polarizing;
s3: controlling and detecting the polarized scattered light intensity information of different angles of the particles in the air sample to be detected after the particles scatter laser and the fluorescence intensity information of different angles of the particles after the particles excite the laser;
s4: analyzing the fluorescence characteristics and polarization characteristics of the particles;
s5: and controlling spectrum demodulation to obtain spectrum information, and distinguishing different air samples to be detected by utilizing different spectrum information.
9. The fluorescent particle detection method of claim 8, wherein the particle test area is circular and the air sample to be tested passes vertically through the center of the particle test area.
10. The fluorescent particle detection method of claim 8, wherein analyzing the fluorescent and polarization characteristics of the particles comprises:
and calculating the ratio of the light intensity signals of the scattered light and the light intensity signals of the fluorescence at different angles to obtain the fluorescence characteristics of the particles, and judging whether the particles are particles capable of generating fluorescence or not.
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| CN102135492A (en) * | 2010-01-25 | 2011-07-27 | 北京汇丰隆生物科技发展有限公司 | Continuous laser particle analyzer |
| CN103630523A (en) * | 2012-08-21 | 2014-03-12 | 杭州希玛诺光电技术有限公司 | Laser induction spectrum generating device used for water quality optical analyzer |
| US9291503B2 (en) * | 2012-11-08 | 2016-03-22 | Hitachi, Ltd. | Flow type single-particle spectrometer |
| CN104089855B (en) * | 2014-07-17 | 2016-08-24 | 清华大学深圳研究生院 | A kind of polarized light scatter measures the method and device of particulate matter |
| CN105675498B (en) * | 2016-03-22 | 2018-08-28 | 上海理工大学 | Fluorescence Raman synchronizes blocky article inspection device |
| US9851291B2 (en) * | 2016-05-02 | 2017-12-26 | Hamilton Associates, Inc. | Realtime optical method and system for detecting and classifying biological and non-biological particles |
| CN108287129A (en) * | 2018-03-22 | 2018-07-17 | 中国计量大学 | The detection device of multichannel fluorescence Spectra bioaerosol particle |
| CN108375530A (en) * | 2018-03-28 | 2018-08-07 | 南京工业大学 | A kind of bioaerosol real-time detection method and device based on laser induced fluorescence |
| CN108844865A (en) * | 2018-04-20 | 2018-11-20 | 清华大学深圳研究生院 | A kind of method and device of dual wavelength polarized light scatter measurement particulate matter |
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